JP2000169320A - Cell activation agent and preparation for external use for skin and skin cleaning agent containing the activation agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cell activation agent having high safety, exhibiting excellent cell activation effect and useful for the improvement of roughened skin, prevention of aging, or the like, by using a specific vegetable extract in combination with yucca extract. SOLUTION: The objective agent contains (A) at least one kind of substance selected from seaweed extract (preferably water extract of the whole body of brown algae), chamomile extract (preferably water extract of chamomile flower), soapberry extract (water and/or ethanol extract of the pericarp of soapberry) and extract of Stephania cepharantha (preferably containing cepharanthine) and (B) yucca extract. A compounding weight ratio of A and B (A:B) is preferably 9:1 to 1:9. A preparation for external use for skin or a cleaning agent effective for the improvement of the roughened skin, prevention of aging, prevention of falling off of hair and promotion of hair growth can be produced by compounding the cell activation agent in an amount of 0.01-50 wt.% based on the total weight of the preparation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a cell activator,
More specifically, aging prevention such as improvement of rough skin and wrinkle formation,
Alternatively, the present invention relates to a cell activator for preventing hair loss in hair and promoting hair growth, and a skin external preparation and a detergent containing the same.

[0002]

2. Description of the Related Art Conventionally, various cell activating components have been used for the purpose of preventing aging such as formation of rough skin and wrinkles, or preventing hair loss or hair growth in hair. The advantage of the cell activator is to promote local cell division, which increases metabolism and improves skin and hair to a healthy state. Specifically, in the skin, keratinocytes and fibroblasts of the epidermis are activated, and at the same time as old keratin is peeled off,
Since the production of collagen and the like that control the elasticity of the skin increases, the skin becomes fresh and firm. On the other hand, in the hair, the hair mother cells and hair papilla cells that control hair growth are activated,
The firm hair that is hard to come off grows. Numerous compounds and natural products are known as cell activating components exhibiting such effects, and in recent years, plant extracts have tended to be used in consideration of safety. However, the activating effects of these cell activating components are relatively moderate, and are still sufficient when the purpose is to prevent aging such as formation of wrinkles for improving rough skin, or to prevent hair loss or promote hair growth in hair. Is not obtained.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cell activator which is highly safe and exhibits excellent cell activating activity. It is still another object of the present invention to provide a skin external preparation and a skin cleansing agent containing such a cell activator and effective for improving rough skin and preventing hair loss in hair.

[0004]

Means for Solving the Problems As a result of intensive studies to achieve the above object, the present inventors have found that at least one selected from the group consisting of a seaweed extract, a chamomile extract, a sapling extract, and a scorpion extract. The present inventors have found that the use of one of these and the yucca extract in combination exhibits an excellent cell activating effect, thereby completing the present invention. Accordingly, the present invention provides a cell activator containing at least one plant extract selected from the group consisting of a seaweed extract, a chamomile extract, a sapling extract, and a Tamasaki diphthora extract, and a yucca extract, and The present invention relates to a formulated external preparation for skin and a skin cleanser. These plant extracts are of very low toxicity.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
First, each plant extract used in the present invention will be described. <Yucca extract> As the raw material Yucca, most of the plants of the genus Yucca can be used, but those which are relatively easy to obtain and are suitable as raw materials are Yucca arizonica, Yucca b
revifolia, Yucca schidigera, Yucca elata, Yucca in
termdia, Yucca recurvifolia Salisb, Yucca smallian
a Fernald, Yucca aloifolia Linn, Yucca gloriosa Li
nn, Yucca mohavensis, Yucca peninsularis, Yucca sc
hottii, Yucca whipplei, etc. One of these may be used, or two or more of them may be used.

[0006] Yucca can be used as an extraction raw material in both underground and above-ground parts (including seeds). The raw material is appropriately pulverized to an appropriate size, and then extracted with water, a hydrophilic organic solvent or a mixture thereof. As the hydrophilic organic solvent for extraction, a lower alcohol such as methanol and ethanol, a polyhydric alcohol such as glycerin, 1,3-butylene glycol and propylene glycol, and acetone are preferable. The extraction method is not particularly limited, and generally, the raw material may be immersed in a solvent maintained at a normal temperature or a temperature near the boiling point. As a method of obtaining a steroid saponin-containing extract without resorting to solvent extraction, there is also a method of squeezing a fresh plant body as a raw material. In addition to using the extract obtained by extraction with a predetermined extraction solvent as it is, the extract may be concentrated or dried to a solid (powder or granule). Further, fractionation is performed using a column filled with an ion exchange resin using water, a hydrophilic organic solvent or a mixture thereof, and an extract containing one or more of saponins, flavonoids, and resveratrol as main components is extracted. May be used. As the extract, those commonly used in foods and the like can be used, and they can be generally obtained on the market. In the present invention, such commercially available products can be used.

<Seaweed extract> Brown algae (Phaeophyta, Lami)
naria digitata, etc.) and red algae (Rhodophyta, Ceramium)
Etc.) and green algae (Chlorophyta), algae and mekabu (spores or adult leaves). The raw material is appropriately pulverized to an appropriate size, and then extracted with water, a hydrophilic organic solvent or a mixture thereof. As the hydrophilic organic solvent for extraction, lower alcohols such as methanol and ethanol, and polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol are preferable. The extraction method is not particularly limited, and generally, the raw material may be immersed in a solvent maintained at a normal temperature or a temperature near the boiling point. In addition to using the extract obtained by extraction with a predetermined extraction solvent as it is, the extract may be concentrated or dried to a solid (powder or granule). Brown algae (Phaeophy
ta, Laminaria digitata, etc.) can preferably be used.

<Chamomile (chamomile) extract> A chamomile (Matricaria chamomilla Linne, Compositae) flower is used as a raw material. Raw materials are appropriately ground to appropriate size,
Extraction treatment is performed with water, a hydrophilic organic solvent or a mixture thereof. As the hydrophilic organic solvent for extraction, lower alcohols such as methanol and ethanol, and polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol are preferable. The extraction method is not particularly limited, and generally, the raw material may be immersed in a solvent maintained at a normal temperature or a temperature near the boiling point. In addition to using the extract obtained by extraction with a predetermined extraction solvent as it is, the extract may be concentrated or dried to a solid (powder or granule). Among them, an aqueous extract of chamomile flowers can be preferably used.

<Mouth extract> Sapindus m
(ukurossi Gaertner, Sapindaceae) as the raw material. The raw material is appropriately pulverized to an appropriate size, and then extracted with water, a hydrophilic organic solvent or a mixture thereof. Suitable hydrophilic organic solvents for extraction include lower alcohols such as methanol and ethanol, and polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol. In particular, water, ethanol, and a mixture of water and ethanol at any ratio can be preferably used. The extraction method is not particularly limited, and generally, the raw material may be immersed in a solvent maintained at a normal temperature or a temperature near the boiling point. In addition to using the extract obtained by extraction with a predetermined extraction solvent as it is, the extract may be concentrated or dried to a solid (powder or granule). As the above-mentioned seaweed extract, chamomile extract and mukurouji extract, those commonly used in cosmetics can be used, and they are generally available on the market.
In the present invention, such commercially available products can be used.

<Tamasaki luffy extract> A tuberculum of Tamasaki luffy (Stcphania cepharantha Hayata) is used as a raw material. The raw material can be extracted according to the above-described extraction method for chamomile or muk roe to obtain an extract. As the T. serrata extract, those containing cepharanthin can be preferably used, and a purified fraction of cepharanthin can be used. In the case where cepharanthin in the contained component is the main extract from T. samurai, the following method can be specifically used. The raw material is cut into a suitable size and dried at 40 ° C. for 24 hours to obtain a coarse powder. This is immersed in methanol for 48 hours, and the obtained immersion liquid is concentrated under reduced pressure to produce a viscous extract. Dilute hydrochloric acid is added to the extract for extraction, and the extract is filtered. The filtrate is adjusted to pH 9 to 10 by adding aqueous ammonia, n-hexane is added, and the mixture is shaken and left. After standing, the n-hexane layer is separated and washed with a 2N sodium hydroxide solution. Next, dilute hydrochloric acid is added to the n-hexane layer and shaken well, and then the aqueous layer is separated. A sodium hydroxide solution is gradually added to the aqueous layer with stirring, and the mixture is allowed to stand at pH 9 to 10. After standing, add ether and shake well. Separate the ether layer,
After washing with water, dehydrate with anhydrous sodium sulfate. The ether layer is evaporated to dryness on a water bath to obtain a dried product. This dried product mainly contains cepharanthin. In the case of the present extract also, those commonly used in hair restorers can be used, and they can be generally obtained on the market.

[0011] The cell activator of the present invention comprises at least one plant extract selected from the group consisting of a seaweed extract, a chamomile extract, a sapling extract, and a Tamasaki diphthora extract.
It contains a seed and a Yucca extract, and each extract can contain one or more. The cell activator referred to in the present invention is not particularly limited, but is preferably applied externally, and its shape is liquid, solid, or semi-solid. The use of the preparation containing the cell activator of the present invention is arbitrary, and is widely used for pharmaceuticals, quasi-drugs, cosmetics, toiletries and the like. For example, skin lotions, creams / emulsions, packs, cosmetic oils, ointments, gels, deodorants, deodorants, hair tonics, hair mist, hair gels, etc. as skin external preparations, and hair shampoos, hair rinses, face wash, body soaps as skin cleansing agents And the like.

The cell activator of the present invention and the external preparation for skin and skin cleansing agent containing the same can be mixed with a base component and a medicinal component generally used for cosmetics and the like according to the purpose. Specifically, mineral oil, animal and vegetable oils, waxes, fatty acids, fatty alcohols, ester oils, surfactants, wetting agents, high molecular compounds, animal and plant extracts, amino acids, solvents, vitamins, anti-inflammatory agents, preservatives, UV absorbers, sequestering agents, antioxidants, pH adjusters, dyes
Examples include pigments and fragrances, which can be blended within a range that does not impair the effects of the present invention. The external preparation for skin and the skin cleansing agent of the present invention can be produced according to a conventional method according to the kind. In addition, each plant extract to be used is, for example, in the form of a concentrated solution or a solid such as a powder or granules, and it may be difficult to handle it in its original form for incorporation into a skin external preparation or a skin cleanser. It can also be blended after being diluted or dissolved with a solvent.

Specific examples of the above various additives are shown below. Mineral oil Liquid paraffin, liquid isoparaffin, etc.Animal and vegetable oils Squalane, olive oil, camellia oil, wheat germ oil, jojoba oil, avocado oil, carrot oil, shea butter, palm oil, hydrogenated oil, horse oil, lanolins, egg yolk oil, clove Oil, rosehip oil, lavender oil, peppermint oil, spearmint oil,
Rosemary oil, etc. Wax Microcrystalline wax, solid paraffin, beeswax, etc. Fatty acids Lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, etc.

Fatty alcohols lauryl alcohol, myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol,
Hexyldecanol, behenyl alcohol, octyldodecanol, lanolin alcohol, etc. Ester oils glyceryl tricabrylate, setell 2-ethylhexanoate, glyceryl tri-2-ethylhexanoate, isocetyl octanoate, isononyl isononanoate, ethylene glycol dioctanoate, tri (caprylic caprin) Acids) glycerin, isopropyl myristate, isopropyl palmitate, isocetyl myristate, cetyl palmitate, dialkyl carbonate, etc.

Surfactants Lauryl sulfate, alkyl sulfate, polyoxyethylene alkyl sulfate, tetradecene sulfon lauryl sulfate, alkyl sulfate, polyoxyethylene alkyl sulfate, tetradecene sulfonate, polyoxyethylene alkyl sulfosuccinate Acid salt, lauroyl sarcosine salt, alkylmethyl-β-alanine salt, polyoxyethylene alkyl ether phosphate, fatty acid soap, N-acylglutamate, lauric acid diethanolamide, coconut oil fatty acid diethanolamide, alkyldimethylamine oxide, alkyl Methyl taurine salt, alkylamino propionate, polyoxyethylene alkyl ether carboxylate, alkyl phosphate, alkyl glucoside,
Polyether-modified silicon, etc. Alkyl trimethyl ammonium chloride, alkyl trimethyl ammonium bromide, amidoamine, dialkyl dimethyl ammonium chloride alkyl dimethyl acetate betaine, alkyl amide propyl betaine, alkyl carboxymethyl hydroxyethyl imidazolinium betaine lecithin (soy or egg yolk) derivative, propylene Glycol fatty acid ester, glycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbit fatty acid ester, polyoxyethylene hardened castor oil, polyethylene glycol fatty acid ester, Polyoxyethylene alkyl ether, poly Alkoxy polyoxypropylene alkyl ethers, polyoxyethylene alkyl nonylphenyl ether, polyoxyethylene alkyl ether phosphoric acid-phosphate

<Wetting agent> Propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, diglycerin, isoprene glycol, polyethylene glycol, sorbit, maltitol, trehalose, xylit, etc. <Polymer compound> Methyl cellulose, ethyl cellulose, Carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, cationized cellulose, cationized guar gum, sodium hyaluronate, sodium polyacrylate, xanthan gum, carrageenan, etc. <amino acids> L-alanine, L -Arginine, L-aspartic acid, L-glutamine, L-cysteine, L-
Serine, L-tyrosine, L-proline, pyrrolidone carboxylate, glycine, etc. <Solvent> Purified water, ordinary water, ethanol, isopropanol, benzyl alcohol, etc.

<Anti-inflammatory agent> Glycyrrhizic acid, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, glycyrrhetinic acid, stearyl glycyrrhetinate,
Guaiazulene, sodium guaiazulene sulfonate, allantoin, ε-aminocaproic acid, etc. <Preservatives> Methylparaben, ethylparaben, propylparaben, butylparaben, isobutylparaben, phenoxyethanol, bisabolol, hinokitiol, benzoic acid, sodium benzoate, salicylic acid, sodium salicylate, Sorbic acid, potassium sorbate, undecylenic acid, pionin, 1-menthol, d-camphor and the like

<Ultraviolet absorber> Paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, 2-ethylhexyl paradimethylaminobenzoate,
Oxybenzone, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, sodium dihydroxydimethoxybenzophenonesulfonate, sodium hydroxymethoxybenzophenonesulfonate, octyl salicylate, etc. <sequestering agents> edetic acid, edetate, trisodium ethylenediaminehydroxyethyltriacetate, diethylenetriaminepentaacetic acid Pentasodium diethylenetriaminepentaacetic acid, ethylenediaminetetrakis (2-hydroxyisopropyl) dioleate, hydroxyethanedisulfonic acid, tetrasodium hydroxyethanedisulfonate, phytic acid, etc. <Antioxidant> dibutylhydroxytoluene, butylhydroxyanisole, erythorbic acid, Propyl gallate, octyl gallate, d-δ-toco Ferrol, etc.

In the cell activator of the present invention, at least one plant extract (A) selected from the group consisting of a seaweed extract, a chamomile extract, a sapling extract, and a scorpion extract, and a yucca extract ( The compounding ratio (A: B) with B) is suitably 9: 1 to 1: 9 by weight,
Preferably it is 3: 7 to 8: 2. When the ratio of the Yucca extract is smaller than 9: 1, almost no cell activating activity is observed, and when it is larger than 1: 9, the cell activating activity is also poor. Also, a skin external preparation of the cell activator of the present invention,
The compounding amount in the skin cleansing agent is 0.01% by weight (hereinafter,%) based on the total weight of the skin external preparation or the skin cleansing agent.
%, More preferably 0.1 to 20%. 0.
If it is less than 01%, the cell activation activity is poor, and if it exceeds 50%, it is disadvantageous in terms of cost.

[0020]

Next, the present invention will be described in more detail by way of examples. The present invention is not limited by this. Using the following extracts, the cell activators of Examples and Comparative Examples were prepared. In addition, each example is abbreviated according to the extract which comprises a cell activator. Example 1: Isoconcentrations (w / v) of Tamasakitsu rafuji extract (Cepharanthin, manufactured by Maruzen Pharmaceutical Co., Ltd.) and Yucca extract (Yucca Sarasaponin 80-M, manufactured by Mitsuba Trading Co., Ltd.)
%) Equal volume mixture, abbreviation [T + Y1] Example 2: Chamomile extract (chamomile extract W, manufactured by Maruzen Pharmaceutical Co., Ltd.) and Yucca extract (Yuca sarasaponin 80)
-M, an equal volume mixture (w / v%) of Mitsuba Trading Co., Ltd., abbreviation [K + Y1] Example 3: Mukuroji extract (mukuroji extract powder,
Equal concentrations (w / v%) of a mixture of Maruzen Pharmaceutical Co., Ltd.) and Yucca extract (Yucca Sarasaponin 80-M, manufactured by Mitsuba Trading Co., Ltd.), equal volume mixture, abbreviation [M + Y1] Example 4: Seaweed Extract (Phycostimulant ARL: Phyco AR
LEDTA, manufactured by codif) and yucca extract (Yucca salsaponin 80-M, manufactured by Mitsuba Trading Co., Ltd.) (w / v%)
Mixture of equal volumes, abbreviation [S1 + Y1]

Example 5: Seaweed extract (Concentre phycos)
timulant: Equal concentration mixture (w / v%) of C.Phyco, manufactured by codif and Yucca extract (Yuca sala saponin 80-M, manufactured by Mitsuba Trading Co., Ltd.), equal volume mixture, abbreviation [S2 + Y1] 6: Mukuroji extract (mukuroji extract powder,
Equal concentration mixture (w / v%) of a Yucca extract (Yukka Sarasaponin 50-M, manufactured by Mitsuba Trading Co., Ltd.) and an abbreviated symbol [M + Y2] abbreviation [M + Y2] Example 7: Chamomile Extract (chamomile extract W, manufactured by Maruzen Pharmaceutical Co., Ltd.) and yucca extract (Yucca salsaponin 50
-M, an equal volume mixture (w / v%) of Mitsuba Trading Co., Ltd., abbreviation [K + Y2] Example 8: Seaweed extract (Phycostimulant ARL: Phyco AR)
LEDTA, manufactured by codif) and Yucca extract (Yucca salsaponin 50-M, manufactured by Mitsuba Trading Co., Ltd.) (w / v%)
Example 9: seaweed extract (Concentre phycostimulant: C.
Equivalent concentrations (w / v%) of Phyco, codif) and Yucca extract (Yucca salsaponin 50-M, manufactured by Mitsuba Trading Co., Ltd.)
Equal volume mixture, abbreviation [S2 + Y2]

Comparative Example 1: Tamasakitsu Lafuji extract (Sepharanthin, manufactured by Maruzen Pharmaceutical Co., Ltd.), abbreviation [T] Comparative Example 2: Chamomile extract (chamomile extract W, manufactured by Maruzen Pharmaceutical Co., Ltd.), abbreviation [ K] Comparative Example 3: Yucca extract (Yucca salsaponin 80-
M, manufactured by Mitsuba Trading Co., Ltd.), abbreviation [Y1] Comparative Example 4: Yucca extract (Yuca sarasaponin 50-
M, manufactured by Mitsuba Trading Co., Ltd.), abbreviation [Y2] Comparative Example 5: Mukuroji extract (mukuroji extract powder,
Abbreviation [M] Comparative Example 6: Phycostimulant ARL (Phyco AR)
LEDTA, manufactured by codif), abbreviation [S1] Comparative Example 7: Seaweed extract (Concentre phycostimulant: C.
Phyco, codif), abbreviation [S2]

Test of Cell Activating Activity Using the cell activating agents of the above Examples and Comparative Examples, two types of cell activating activities were tested. The method and test results are shown below. (1) Normal human skin fibroblasts (NB1RGB)
Of cell activation activity in NB1RGB (purchased from RIKEN) 10% FB in advance
The cells were cultured in an S-supplemented RITC80-7 medium under the conditions of 5% CO ₂ and 37 ° C. for 6 days. The cultured cells were then PBS (-)
And the cells were detached by treatment with 0.02% EDTA disodium and 0.25% trypsin, and RITC80 with 5% FBS.
5 × 10 ⁴ cells / ml cell suspension was prepared in -7 medium. This was dispensed in 0.2 ml portions into a 96-well microplate, and pre-cultured (24 hours) under the same culture conditions. Each sample was dissolved in ultrapure water, PBS (-) or dimethylsulfoxide (purchased from Wako Pure Chemical Industries, Ltd.) as a solvent, depending on the solubility, and the final concentration was 10, 20, 40, 5 μl of each sample was added to 80 ppm, and the cells were cultured for 48 hours. In the background, 5 μl of only the solvent used was added.

(2) Normal human neonatal foreskin epidermal keratinocytes
(Hereinafter referred to as NHEK (F)) Test of cell activation activity NHEK (F) (purchased from Kurashiki Boseki Co., Ltd.)
Clear culture substrate: HuMedia-KG2 (Kurashiki Boseki Co., Ltd.)
5% CO_TwoCultured at 37 ° C for 6 days
did. The cultured cells are then washed with PBS (-),
0.02% EDTA disodium added 0.25% trypsin treatment
Peel off the cells with 5 × 10 5 ^FourCells / ml cells
A suspension was prepared. Add this to a 96-well microplate for 0.2
Pre-culture (24 hours) under the same culture conditions
Was. Each sample is made of ultra pure water, PB
S (-) or dimethyl sulfoxide (Wako Pure Chemical Industries
(Purchased from Co., Ltd.) as a solvent to a final concentration of 10,
Add 5 μl of each sample to give 20, 40, 80 ppm
Cultured for 48 hours. The background is used for each
Only 5 μl of the solvent was added.

The cell activating activities of the above (1) and (2) were determined by adding Tetracolor One (purchased from Seikagaku Corporation), and 2 hours later, using a spectrophotometer at wavelengths of 450 nm and 6 nm.
Measure absorbance at 20 nm and determine absorbance at 450 nm from 620
The value obtained by subtracting the absorbance at nm was compared with Δabs. Δabs
The larger the value, the higher the cell activation activity. In this test, it was judged that there was excellent cell activating activity when Δabs of those mixtures was higher than Δabs of individual extracts at the same concentration. Hereinafter, Tables 1 and 2 summarize the test results (Δabs values).

[0026]

[Table 1] (1) Cell activating activity in NB1RGB (depending on sample composition) Sample concentration (ppm) Abbreviation 10 20 40 80 Example 1 [T + Y1] 0.514 0.688 0.755 0.732 Example 2 [K + Y1] 0.640 0.627 0.676 0.697 ---------------- -------------------------------------------------- -Comparative Example 1 [T] 0.231 0.337 0.423 0.292 Comparative Example 2 [K] 0.288 -0.344 -0.406 -0.412 Comparative Example 3 [Y1] 0.219 0.271 0.289 0.176

[0027]

[Table 2] (2) Cell activation activity in NHEK (F) ───────────────────────────────── Sample Sample concentration (ppm) according to composition Abbreviation 10 20 40 80 Example 1 [T + Y1] 0.998 1.168 1.236 1.216 Example 2 [K + Y1] 1.004 0.904 0.749 0.616 Example 3 [M + Y1] 0.847 0.778 0.758 0.803 Example 4 [S1 + Y1] 0.832 0.709 0.734 0.540 Example 5 [S2 + Y1] 0.828 0.775 0.793 0.772 Example 6 [M + Y2] 0.525 0.490 0.457 0.470 Example 7 [K + Y2] 0.710 0.742 0.729 0.765 Example 8 [ S1 + Y2] 0.760 0.688 0.641 0.548 Example 9 [S2 + Y2] 0.700 0.776 0.749 0.685 ------------------------------ ------------------------------------ Comparative Example 1 [T] 0.578 0.633 0.559 0.509 Comparative Example 2 [ K] 0.227 0.092 0.061 0.100 Comparative Example 3 [Y1] 0.148 0.151 0.171 0.194 Comparative Example 4 [Y2] 0.188 0.183 0.186 0.192 Comparative Example 5 [M] 0.252 0.232 0.181 0.179 Comparative Example 6 [S1] 0.255 0.267 0.175 0.124 Comparative Example 7 [ S2] 0.346 0.253 0.231 0.156

As is clear from the results in the above table, the cell activating agents of the examples exhibited excellent cell activating activities in NB1RGB and NHEK (F). Compared with the effect of each plant extract alone (Comparative Examples 1 to 7), it can be seen that the combined use with the Yucca extract in the Examples exerts a synergistic effect.

Usage test (1) Skin quality and fine wrinkle improvement effect (monitor test) A lotion (Example 10) containing a cell activator comprising a combination of chamomile extract and Yucca extract, and a makeup for comparison Water (Comparative Examples 8 and 9) was prepared with the composition shown in Table 3 (unit is parts by weight), and the following tests were performed. Ten healthy adult women (25 to 35 years old) were used as test subjects, and the lotion of each case was applied to the skin of the face for one day (morning and evening use) for one month, followed by a) the effect of giving firmness to the skin, b) dullness , The effect of improving spots and c) the effect of improving fine wrinkles were investigated. All of these effects were observed by visually observing the skin condition and evaluated according to the following criteria. Table 4 summarizes the number of people who made each determination. <Criteria> A: Very effective. B: There was an effect. C: No change. D: Slightly worse. E: It got worse.

[0030]

Table 3 Composition Example 10 Comparative Example 8 Comparative Example 9 1 ) Chamomile extract 7.0 7.0 --- 2) Yucca extract 3.0 --- 3.0 3) 1,3-butylene glycol 5.0 5.0 5.0 4) Ethanol 15 5.0 15.0 15.0 5) Methylparaben 0.1 0.1 0.1 6) The above-mentioned 1) to 6), whose total amount was 100 in purified water, were uniformly mixed and dissolved to prepare a lotion.

[0031]

[Table 4] Monitor test results ハ リSkin firmness / stain improvement ABCDEABCDEABCDE embodiment of improved fine wrinkles 10 8 1 1 0 0 3 5 2 0 0 9 1 0 0 0 Comparative example 8 2 2 6 0 0 1 2 4 1 0 1 1 8 0 0 Comparative example 9 1 1 7 1 0 2 17 0 0 0 3 7 0 0 The numbers in the table represent the number of people. As is clear from the above table, it can be seen that the use of the lotion of Example 10 improves the firmness of the skin and improves dullness / stain and fine wrinkles. No subject had any abnormal skin during the monitor test.

(2) Effect of improving scalp and hair (monitor test) Hair tonic (Example 11) containing a cell activator comprising a combination of Tamasaki tsuta extract and Yucca extract (Example 11) and a hair tonic for comparison (Comparative Examples 10 and 11) were prepared with compositions (units by weight) shown in Table 5,
The following tests were performed. Healthy adult men (25-45
10 years old) After using the hair tonic of each case on the scalp for 1 month every day (morning and evening use) for 10 months, a) reduction of hair loss, b) improvement of stiffness and gloss, c) dandruff and itch The reduction effect was investigated. All of these effects were evaluated according to the following criteria. Table 6 summarizes the number of people who made each determination. <Evaluation Criteria> A: Very effective. B: There was an effect. C: No change. D: Slightly worse. E: It got worse.

[0033]

Table 5 Example 11 Comparative Example 10 Comparative Example 11 1) Tamasaki Luffy extract 2.0 2.0 ---- 2) Yucca extract 8.0 ------ 8.0 3) Propylene glycol 5.0 5.0 5.0 4) Ethanol 65.0 65 5) Methyl paraben 0.1 0.1 0.1 6) The above-mentioned 1) to 6) having a total amount of 100 with purified water were uniformly mixed and dissolved to obtain a hair tonic.

[0034]

[Table 6] of the monitor test results ─────────────────────────────────── of reduction Cosi luster of hair loss reduction of improving dandruff itching ABCDEABCDEABCDE example 11 8 2 0 0 0 4 4 2 0 0 7 2 1 0 0 Comparative example 10 1 2 6 1 0 1 3 6 0 0 2 2 6 0 0 Comparative example 11 1 1 7 1 0 0 2 7 1 0 1 3 6 0 0 The numbers in the table represent the number of people.

As apparent from Table 6 above, Example 1
It can be seen that the use of Hair Tonic No. 1 reduces hair loss, improves stiffness and gloss of hair, and reduces dandruff and itch. In addition, none of the subjects had abnormalities on the scalp and hair during the monitor test.

Example 12 Preparation of lotion A lotion was prepared according to the following composition (unit: parts by weight).
The total amount was adjusted to 100 with purified water. 1. 1. Polyoxyethylene (60) hydrogenated castor oil 0.8 Glycerin 3.0 3. Dipropylene glycol 2.0 4. Ethanol 5.0 5. 5. Cell activator 0.1 (Mokusuji extract / Yuca extract = 1/9) Methyl paraben 0.2 7. Purified water Remainder Manufacturing method: Components 1 to 7 are uniformly mixed and dissolved to make a lotion.

Example 13 Preparation of Cream A cream was prepared according to the following composition (unit: parts by weight). The total amount was adjusted to 100 with purified water. 1. 1. Sodium polyoxyethylene (10) alkyl ether phosphate 4.5 2. Polyoxyethylene (3) alkyl ether 2.0 3. Lipophilic glyceryl monostearate 2.0 Cetanol 2.05. Squalane 5.0 6. Liquid paraffin 10.0 7.1 1,3-butylene glycol 5.0 8. Hydrolyzed soy protein 0.5 9. Sodium hyaluronate solution (1%) 1.0 10. Methyl paraben 0.2 11. Propylparaben 0.1 12. Cell activator 0.01 (seaweed extract / Yucca extract = 7/3) 13. Purified water Remaining Production method: Components 1 to 7 and 10, 11 are mixed by heating at 80 ° C, and 13 heated to the same temperature is gradually added to emulsify. 40 ℃
And add ingredients 8, 9 and 12 and stir, then cool to near room temperature to make a cream.

Example 14 Preparation of Emulsion An emulsion was prepared according to the following composition (unit: parts by weight). The total amount was adjusted to 100 with purified water. 1. 1. Polyoxyethylene (20) behenyl ether 1.2 2. Polyoxyethylene (60) sorbite tetraoleate 1.2 3. Lipophilic glyceryl monostearate 1.0 Cetostearyl alcohol 0.5 5.1 1,3-butylene glycol 6.0 6. Liquid paraffin 8.0 7. 7. vegetable squalane 3.0 8. Isocetyl myristate 2.0 Phenoxyethanol 0.5 10. Methyl paraben 0.3 11. Propylparaben 0.1 12. Carboxyvinyl polymer 0.1 13. Xanthan gum (1% aqueous solution) 10.0 14. Potassium hydroxide (10% aqueous solution) 0.47 15. Cell activator 20.0 (chamomile extract / Yucca extract = 2/8) Purified water Remaining Production method: Components 1 to 11 are uniformly heated and mixed at 80 ° C, and Component 16 at the same temperature is gradually added to emulsify. After adding and mixing the component 12, the component 14 is added to make the mixture uniform. Next, the mixture is cooled to 50 ° C., and the components 13 and 15 are added.

Example 15 Preparation of Face Wash Foam A face wash foam was prepared according to the following composition (unit: parts by weight). The total amount was adjusted to 100 with purified water. 1. Myristic acid 20.0 2. Palmitic acid 5.0 3. Lauric acid diethanolamide 3.5 4. 4. Polyethylene glycol distearate (140) 1.0 Polyethylene glycol 400 5.0 6. 6. N-lauroylmethyltaurine sodium solution 8.0 Glycerin 20.0 8. Sorvit liquid 3.0 9. Disodium edetate 0.05 10. Dibutylhydroxytoluene 0.05 11. Potassium hydroxide 5.3 12. Isopropyl methylphenol 0.2 13. Fragrance 0.1 14. Cell activator 1.0 (seaweed extract / Yucca extract = 9/1) Purified water Remaining Production method: Components 1 to 10 and 12 are uniformly heated and mixed at 80 ° C.
A solution obtained by dissolving the component 11 in the component 15 is gradually added thereto. After sufficiently stirring, the mixture is cooled to 45 ° C., and the components 13 and 14 are added.

Example 16 Preparation of Hair Shampoo A hair shampoo was prepared according to the following composition (unit: parts by weight). The total amount was adjusted to 100 with purified water. 1. 1. Polyoxyethylene (3) sodium lauryl ether sulfate 20.0 2. Palm oil fatty acid amidopropyl betaine solution 10.0 Lauric acid diethanolamide 3.5 4. 4. Ethylene glycol distearate 1.0 Lauroyl sarcosine sodium solution 8.0 6. Jojoba oil 0.2 7. Glycerin 1.0 8. Methyl paraben 0.29. Disodium edetate 0.1 10. Tocopherol acetate 0.05 11. Citric acid qs. Cationized cellulose (1% aqueous solution) 20.0 13. Fragrance 0.2 14. Cell activator 0.5 (Mokusuji extract / Yucca extract = 5/5) Purified water Remaining Production method: Components 1 to 10 are uniformly mixed at 75 ° C, and Component 15 heated to the same temperature is gradually added thereto. Then component 11 and
After adding 12 and mixing uniformly, cool to 50 ° C.
Add 14 and cool to near room temperature to make shampoo.

Example 17 Preparation of Hair Restorative A hair restorer was prepared according to the following composition (unit: parts by weight).
The total amount was adjusted to 100 with purified water. 1. Polyoxyethylene (60) hydrogenated castor oil 0.3 2. Propylene glycol 3.0 3. Glycyrrhetinic acid 0.2 4. Pantothenyl alcohol 0.55. Carrot extract 2.0 6. Showa tincture 0.57. 7. Tocopherol acetate 0.1 Ethanol 30.0 9. Cell activator 50.0 (Tamasaki tsuta extract / Yucca extract = 1/9) Methyl paraben 0.2 11. Purified water Remainder Manufacturing method: Components 1 to 11 are mixed and dissolved uniformly to prepare a hair restorer.

Each of the skin external preparations and cleaning agents of Examples 12 to 17 is excellent in the effects of improving skin roughness, preventing aging such as wrinkle formation, preventing hair loss in hair and promoting hair growth under ordinary use conditions. I was

[0043]

The active ingredient of the cell activator of the present invention is plant-derived and highly safe, and has excellent cell-activating activity on human-derived fibroblasts and keratinocytes. Furthermore, the skin external preparation and the cleansing agent containing the cell activator are extremely effective against rough skin and wrinkles formed due to disturbance of the activity of the skin including the scalp, or hair loss and poor growth of the hair.

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Claims (7)

[Claims] 1. A cell activator comprising at least one member selected from the group consisting of a seaweed extract, a chamomile extract, a sapling extract, and a Tamasaki diphthora extract, and a yucca extract. 2. The cell activator according to claim 1, wherein the seaweed extract is an aqueous extract of all algae of brown algae. 3. The cell activator according to claim 1, wherein the chamomile extract is a water extract of chamomile flowers. 4. The method of claim 1 wherein the extract is a sapling (Sapindus).
mukurossi Gaertner, Sapindaceae)
Or an ethanol extract.
Item 10. The cell activator according to item 8. 5. The cell activator according to any one of claims 1 to 4, wherein the T. samurai extract contains cepharanthin. 6. An external preparation for skin containing the cell activator according to any one of claims 1 to 5. 7. A skin cleanser containing the cell activator according to any one of claims 1 to 5.