JP2003028993A - Producing method for biochemical analysis data and scanner used therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a producing method for biochemical analysis data, by uniquely combining a material derived from a living body, marked with radioactive marking materials which wakes materials couple, of which the base sequence, base length, composition are known. SOLUTION: In the method for producing biochemical analysis data, after selectively accumulating radiation energy in a plurality of dotted stimulable phosphor layer region 12 formed mutually apart on a support 11 of an accumulable phosphor sheet 10, excitation light is irradiated in turn on the doted stimulable phosphor layer region to excite the stimulable phosphor included in the region, discharged stimulable phosphor 45 is photochemically detected, analog signal is produced and the analog signal is converted to digital signal. The excitation light is irradiated so that the energy of the excitation light irradiated on the dotted stimulable phosphor layer region per unit area is higher than the region other than that of the dotted stimulable phosphor layer region.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for generating biochemical analysis data and a scanner used therefor, and more specifically, it is capable of specifically binding to a substance of biological origin and having a nucleotide sequence or By selectively binding a substance of biological origin labeled with a radiolabeling substance to a specific binding substance whose base length, composition, etc. are known, the selectively labeled spot-shaped region is used as a carrier such as a membrane filter. Even when formed on the surface with high density, the radioactive labeling substance contained in the spot-shaped region scans the exposed photostimulable phosphor layer with excitation light, and is emitted from the photostimulable phosphor layer. The present invention relates to a biochemical analysis data generation method capable of photoelectrically detecting stimulated emission and generating biochemical analysis data with excellent quantitativeness, and a scanner used therefor. That.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Further, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0007] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0008]

However, in the macroarray analysis system using the radiolabeled substance, when the stimulable phosphor layer is exposed by the radiolabeled substance, the surface of the carrier such as a membrane filter is exposed. The radioactive energy of the radio-labeled substance contained in the formed spot-shaped region was very large, so the electron beam emitted from the radio-labeled substance was scattered and released from the radio-labeled substance contained in the spot-shaped region. Electrons emitted from the radiolabeled substance incident on the region of the stimulable phosphor layer other than the region to be exposed by the electron beam or adhering to the surface of the carrier such as the membrane filter between the adjacent spot-like regions. The rays are incident on the photostimulable phosphor layer, which results in
Noise is generated in the data for biochemical analysis generated by photoelectrically detecting photostimulable light, which makes it difficult to separate the data between adjacent spot-like regions, which lowers the resolution and When quantifying the radiation dose in the spot-shaped area and analyzing the substance derived from the living body, there is a problem that the quantitativeness deteriorates. In particular, the resolution is remarkably lowered, and the quantitativeness is remarkably deteriorated.

Further, by using a scanner and exciting light,
Scanning the stimulable phosphor layer, exciting the stimulable phosphor contained in the stimulable phosphor layer, photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer. Then, when generating the data for biochemical analysis, along with the scanning of the excitation light, the adjacent region of the stimulable phosphor layer to be excited next is excited and emits the stimulable light. Since the accumulated radiation energy is released, when the analysis is performed based on the biochemical analysis data generated by the conventional scanner, there is also a problem that the quantitativeness is inevitably deteriorated.

Therefore, according to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like is labeled with a radiolabeling substance. By specifically binding the substance of origin,
The selectively labeled spot-shaped region, on the surface of a carrier such as a membrane filter, even when formed at high density, by the radioactive labeling substance contained in the spot-shaped region, the exposed photostimulable phosphor layer, Generation of biochemical analysis data capable of generating highly quantitative biochemical analysis data by scanning with excitation light and photoelectrically detecting the photostimulated light emitted from the photostimulable phosphor layer. It is an object of the present invention to provide a method and a scanner used therefor.

[0011]

The object of the present invention is to:
A plurality of dot-shaped stimulable phosphor layer regions provided on the support, the plurality of dot-shaped stimulable phosphor sheet regions formed at least one-dimensionally apart from each other on the support. In the exhaustive phosphor layer region, after selectively storing radiation energy, the stimulable phosphor sheet and excitation light,
At least in the main scanning direction, relatively moved, to the plurality of dot-shaped stimulable phosphor layer regions, sequentially irradiated with the excitation light, the plurality of dot-shaped stimulable phosphor layer regions To excite the stimulable phosphor contained in, photoelectrically detect the stimulable light emitted from the stimulable phosphor to generate an analog signal, and convert the analog signal into a digital signal. And a method for generating biochemical analysis data, which is characterized by generating biochemical analysis data.

According to the present invention, the stimulable phosphor sheet is
Since a plurality of dot-shaped photostimulable phosphor layer regions are formed on the support at least one-dimensionally separated from each other, the support can specifically bind to a substance derived from a living body. In addition, a biologically-derived substance labeled with a radioactive labeling substance is specifically bound to a specific binding substance whose base sequence, base length, composition, etc. are known, by hybridization, etc. Even when the spots marked on the carrier surface such as a membrane filter are formed at a high density, a plurality of dot-shaped stimulable phosphor layer regions are the same as the spots formed on the carrier surface such as a membrane filter. A radioactive label contained in each spot when a stimulable phosphor sheet and a membrane filter are superposed by forming them on a support in a pattern and exposed. The electron beam emitted from the dot-shaped stimulable phosphor layer region other than the dot-shaped stimulable phosphor layer region to be exposed by the electron beam emitted from the radioactive labeling substance contained in the spot Can be reliably prevented from being incident on the dot-shaped photostimulable phosphor layer region. By photoelectrically detecting the photostimulable light emitted from the photocatalyst, it becomes possible to generate data for biochemical analysis with high resolution and high quantitativeness.

In a preferred embodiment of the present invention, the energy per unit area of the excitation light with which the plurality of dot-shaped stimulable phosphor layer regions are irradiated is the plurality of dot-shaped stimulable phosphors. The excitation light is irradiated so as to be higher than the region other than the layer region, and the data for biochemical analysis is generated.

According to a preferred embodiment of the present invention, the stimulable phosphor sheet and the excitation light are relatively moved in at least the main scanning direction to form a plurality of dot-shaped stimulable phosphor layer regions. Sequentially, excitation light is irradiated to excite the stimulable phosphor contained in a plurality of dot-shaped stimulable phosphor layer regions, and the stimulable light emitted from the stimulable phosphor is photoelectrically converted. To generate an analog signal, convert the analog signal into a digital signal, and generate data for biochemical analysis. Excitation applied to a plurality of dot-shaped stimulable phosphor layer regions. Energy per unit area of light is higher than that of a region other than a plurality of dot-shaped stimulable phosphor layer regions, and is configured to irradiate excitation light. Adjacent dots of stimulable fireflies to be excited next with scanning It is possible to reliably prevent the body region from being excited and emitting stimulated emission to release the stored radiation energy, and thus, as desired, a highly quantitative biochemical analysis. It becomes possible to generate data for use.

In a preferred embodiment of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are two-dimensionally formed on the support so as to be spaced apart from each other, and the stimulable phosphor sheet is formed. And excitation light are relatively moved in the main scanning direction and the sub-scanning direction orthogonal to the main scanning direction, and the plurality of dot-shaped stimulable phosphor layer regions are sequentially irradiated with the excitation light. Then, the stimulable phosphor contained in the plurality of dot-shaped stimulable phosphor layer regions is excited, and the stimulable light emitted from the stimulable phosphor is photoelectrically detected. , Is configured to generate an analog signal, convert the analog signal into a digital signal, and generate biochemical analysis data.

According to a preferred embodiment of the present invention, dot-shaped photostimulable phosphor layer regions can be formed at high density, and biochemical analysis data can be efficiently generated. Become.

In a preferred embodiment of the present invention, the stimulable phosphor sheet and the excitation light are relatively and intermittently moved in the main scanning direction, and the plurality of dot-shaped photostimulations are carried out. The fluorescent phosphor layer region is configured to be irradiated with the excitation light for a predetermined time.

According to a preferred embodiment of the present invention, the stimulable phosphor sheet and the exciting light are relatively and intermittently moved in the main scanning direction to form a plurality of dot-shaped stimulable fluorescent materials. Since the body layer regions are each configured to be irradiated with excitation light for a predetermined time, adjacent dot-shaped stimulable phosphors to be excited next with the scanning of excitation light. It is possible to reliably prevent the layer regions from being excited and emitting stimulated emission to release the stored radiation energy, and thus, as desired, for quantitative biochemical analysis. It becomes possible to generate data.

[0019] In a further preferred aspect of the present invention, the excitation light is constantly supplied while the storage phosphor sheet and the excitation light are relatively and intermittently moved in the main scanning direction. , Is configured to irradiate the stimulable phosphor sheet.

According to a further preferred aspect of the present invention, the stimulable light is constantly applied to the stimulable phosphor sheet, but the stimulable phosphor sheet and the stimulable light are relatively irradiated in the main scanning direction. And, because it is moved intermittently, the adjacent dot-shaped stimulable phosphor layer regions to be excited next are excited with the scanning of the excitation light and emit the stimulable light. As a result, it is possible to reliably prevent the accumulated radiation energy from being released, and thus it is possible to generate biochemical analysis data with excellent quantitativeness as desired.

In another preferred embodiment of the present invention, only in the plurality of dot-shaped stimulable phosphor layer regions,
The excitation light is irradiated with the excitation light, and the excitation light is controlled to be turned on / off so that the regions other than the plurality of dot-shaped stimulable phosphor layer regions are not irradiated with the excitation light.

According to another preferred embodiment of the invention,
Excitation light is irradiated only to the multiple dot-shaped stimulable phosphor layer regions, and excitation light is turned on so that the excitation light is not irradiated to regions other than the multiple dot-shaped stimulable phosphor layer regions.・
Since it is configured to be off-controlled, only the dot-shaped stimulable phosphor layer region to be excited is irradiated with the excitation light at each time point, and therefore the excitation light is not scanned. It is even more certain that the adjacent dot-shaped photostimulable phosphor layer regions to be excited next are excited and emit photostimulable light to release the accumulated radiation energy. Therefore, it is possible to generate biochemical analysis data with excellent quantitativeness as desired.

[0023] In a further preferred aspect of the present invention, the stimulable phosphor sheet and the excitation light are arranged at a pitch equal to a distance between the dot-shaped stimulable phosphor layer regions adjacent in the main scanning direction. It is configured to move relatively in the main scanning direction and intermittently.

[0024] In a preferred aspect of the present invention, the stimulable phosphor sheet and the excitation light are moved relatively and continuously in the main scanning direction so that the plurality of dot-like dots are substantially formed. Only the stimulable phosphor layer region of the, the excitation light is irradiated, the region other than the plurality of dot-shaped stimulable phosphor layer region, so that the excitation light is not irradiated, the excitation light is turned on. -It is configured to control off.

According to a preferred embodiment of the present invention, the stimulable phosphor sheet and the excitation light are relative to each other in the main scanning direction.
And, while being continuously moved, substantially only a plurality of dot-shaped stimulable phosphor layer regions, the excitation light is irradiated, a plurality of dot-shaped stimulable phosphor layer regions other than The region is configured to control the excitation light to be turned on and off so that it is not irradiated with the excitation light. It can be reliably prevented that the phosphor layer region is excited and emits photostimulable light to release the accumulated radiation energy, and thus, as desired, a highly quantitative biochemistry. It becomes possible to generate data for analysis.

In a preferred aspect of the present invention, the analog signal is integrated, and the integrated value of the analog signal is digitized to generate biochemical analysis data.

According to a preferred embodiment of the present invention, excitation light is irradiated to excite the stimulable phosphor contained in the plurality of dot-shaped stimulable phosphor layer regions, thereby stimulating the stimulable fluorescence. It is configured to photoelectrically detect stimulated light emitted from the body, integrate the generated analog signal, digitize the integrated value of the analog signal, and generate biochemical analysis data. When the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region is excited by the excitation light, the radiation energy accumulated in the dot-shaped stimulable phosphor layer region is small. In addition, even if the intensity of the stimulating light emitted from the dot-shaped stimulable phosphor layer region is small, the sensitivity is high,
It is possible to generate digital data with sufficiently large signal strength.

[0028] In a preferred embodiment of the present invention, the digital signals are added to generate biochemical analysis data.

According to a preferred embodiment of the present invention, excitation light is irradiated to excite the stimulable phosphor contained in the plurality of dot-shaped stimulable phosphor layer regions, thereby stimulating the stimulable fluorescence. It is configured to photoelectrically detect stimulated light emitted from the body, add digital signals obtained by digitizing the generated analog signals, and generate biochemical analysis data. Radiation energy accumulated in the dot-shaped stimulable phosphor layer region is small, by excitation light, when the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region is excited Even if the intensity of the photostimulable light emitted from the dot-shaped photostimulable phosphor layer region is small, it is possible to generate digital data with high sensitivity and sufficiently high signal intensity.

In a preferred embodiment of the present invention, laser light is used as excitation light, and the stimulable phosphor sheet and the laser light are relatively moved in the main scanning direction and the sub scanning direction orthogonal to the main scanning direction. A plurality of dot-shaped stimulable phosphor layer regions, sequentially irradiated with the laser light, the plurality of dot-shaped stimulable phosphor layer regions stimulable Is configured to excite the fluorescent phosphor.

In a preferred aspect of the present invention, the stimulable phosphor sheet is configured to move in the main scanning direction.

In another preferred embodiment of the present invention, the excitation light is configured to move in the main scanning direction.

In a preferred embodiment of the present invention, a plurality of holes are formed in the support of the stimulable phosphor sheet so as to be separated from each other, and the plurality of dot-shaped stimulable phosphor layer regions are formed. The stimulable phosphor is embedded in the plurality of holes to be formed.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is formed with a plurality of through holes spaced apart from each other, and the plurality of dot-shaped stimulable phosphor layers are formed. A region is formed by embedding a stimulable phosphor in the plurality of through holes.

In a preferred embodiment of the present invention, a plurality of recesses are formed in the support of the stimulable phosphor sheet so as to be spaced apart from each other, and the plurality of dot-shaped stimulable phosphor layer regions are formed. The stimulable phosphor is embedded in the plurality of recesses.

In another preferred embodiment of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the surface of the support of the stimulable phosphor sheet.

In a preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet in a regular pattern.

In a preferred embodiment of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is
It is formed in a substantially circular shape.

In a preferred embodiment of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is
It is formed in a substantially rectangular shape.

In a preferred embodiment of the present invention, 10 or more dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.

[0041] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is formed with 100 or more dot-shaped stimulable phosphor layer regions.

[0042] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is provided with 1000
The above-described dot-shaped stimulable phosphor layer region is formed.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet has 1000
0 or more dot-shaped stimulable phosphor layer regions are formed.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet has 1000
00 or more dot-shaped stimulable phosphor layer regions are formed.

In a preferred embodiment of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are respectively
It is formed to a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is formed in a size of less than 1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is formed in a size of less than 0.5 mm 2.

[0048] In a further preferred aspect of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is formed in a size of less than 0.1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is formed in a size of less than 0.05 mm 2.

[0050] In a further preferred aspect of the present invention, each of the plurality of dot-shaped stimulable phosphor layer regions is formed in a size of less than 0.01 mm 2.

In a preferred embodiment of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 10 / cm 2 or more. ing.

In a further preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 50 or more per cm 2. Has been done.

In a further preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 100 or more per cm 2. Has been done.

In a further preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 500 or more per cm 2. Has been done.

In a further preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 1000 pieces / square centimeter or more. Has been done.

In a further preferred aspect of the present invention, the plurality of dot-shaped photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 5000 or more per cm 2. Has been done.

[0057] In a further preferred aspect of the present invention, the plurality of dot-shaped stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet at a density of 10,000 or more per cm 2. Has been done.

In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet is formed of a radiation attenuating material.

According to a preferred embodiment of the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like is treated with a radiolabeling substance. A labeled substance of biological origin is specifically bound by hybridization or the like,
Even when the selectively labeled spots are formed on the carrier surface such as a membrane filter at a high density, a plurality of stimulable phosphor layer regions have the same pattern as the spots formed on the carrier surface such as a membrane filter. Therefore, it is included in each spot because the support is made of a material that attenuates radiation when it is formed on a support, and a stimulable phosphor sheet and a membrane filter are superposed and exposed. Surely prevent the electron beam emitted from the radioactive labeling substance from entering the region of the stimulable phosphor layer other than the region to be exposed by the electron beam emitted from the radioactive labeling substance contained in the spot. Therefore, the exposed photostimulable phosphor layer regions are scanned with the excitation light, and the photostimulable phosphors emitted from the photostimulable phosphor layer regions are scanned. By photoelectrically detected and
It is possible to generate highly quantitative data for biochemical analysis with high resolution.

In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet is such that the radiation supports the radiation by a distance equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions. It is formed of a material having a property of reducing the energy of radiation to 1/5 or less when transmitted through the body.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is irradiated with the radiation by a distance equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions. It is formed of a material having a property of attenuating the energy of radiation to 1/10 or less when transmitted through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is irradiated with the radiation by a distance equal to a distance between adjacent dot-shaped stimulable phosphor layer regions. It is formed of a material having a property of attenuating the energy of radiation to 1/50 or less when transmitted through the support.

[0063] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is irradiated with the radiation by a distance equal to a distance between the adjacent dot-shaped stimulable phosphor layer regions. It is formed of a material having a property of attenuating the energy of radiation to 1/100 or less when transmitted through the support.

[0064] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is irradiated with the radiation by a distance equal to a distance between the dot-shaped stimulable phosphor layer regions adjacent to each other. It is formed of a material having a property of attenuating the energy of radiation to 1/500 or less when transmitted through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is irradiated with the radiation by a distance equal to a distance between adjacent dot-shaped stimulable phosphor layer regions. It is formed of a material having a property of attenuating the energy of radiation to 1/1000 or less when transmitted through the support.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is made of a material selected from the group consisting of metal materials, ceramic materials and plastic materials.

In the present invention, the material for forming the support of the stimulable phosphor sheet is preferably one having a property of attenuating radiation, and the material having a property of attenuating radiation is particularly limited. But not
Both inorganic compound materials and organic compound materials can be used, and metal materials, ceramic materials or plastic materials are particularly preferably used.

In the present invention, as the inorganic compound material which can be preferably used for forming the support of the stimulable phosphor sheet and which can attenuate the radiation, for example, gold, silver, copper, zinc, aluminum, Metals such as titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; oxidation. Metal oxides such as aluminum, magnesium oxide, zirconium oxide;
Inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material which can be preferably used for forming the support of the stimulable phosphor sheet and which can attenuate the radiation, such as polyethylene or Polyolefin such as polypropylene; acrylic resin such as polymethylmethacrylate or butyl acrylate / methylmethacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylons such as nylon 6, nylon 6,6 and nylon 4,10; polyimides; polysulfones;
Polyphenylene sulfide; Silicon resin such as polydiphenyl siloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose Examples include sugars, chitin, chitosan, sumac, polyamide such as gelatin, collagen, keratin, and copolymers of these polymer compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the larger the specific gravity is, the higher the radiation attenuating ability is. Therefore, the support of the stimulable phosphor sheet is preferably formed of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more. , Specific gravity is 1.5g / c
It is particularly preferable that it is formed of a compound material or a composite material of m ³ or more and 23 g / cm ³ or less.

The object of the present invention is also to provide an excitation light source which emits excitation light, and a storage provided with a plurality of dot-shaped stimulable phosphor layer regions formed separately from each other and selectively storing radiation energy. Sample stage on which a fluorescent phosphor sheet can be placed, an irradiation optical system that directs the excitation light emitted from the excitation light source to the sample stage, and the excitation light emitted from the excitation light source, the plurality of Dot-shaped photostimulable phosphor layer region, so as to be sequentially irradiated, the irradiation optical system and the sample stage,
At least in the main scanning direction, a scanning mechanism that moves relatively, and the excitation light emitted from the excitation light source and directed to the sample stage by the irradiation optical system causes the plurality of dot-shaped photostimulable fluorescence. A photodetector that photoelectrically detects emitted photostimulated light when a body layer region is excited and generates an analog signal, and an analog signal generated by the photodetector are digitized to generate a digital signal. And an A / D converter that operates.

According to the present invention, the scanner includes the excitation light source which emits the excitation light and the plurality of dot-shaped stimulable phosphor layer regions which are formed separately from each other and selectively accumulate the radiation energy. A sample stage on which a stimulable phosphor sheet can be placed, an irradiation optical system that directs the excitation light emitted from the excitation light source to the sample stage, and excitation light emitted from the excitation light source causes multiple dots to be stimulated. Fluorescent phosphor layer region, so that the irradiation optical system and the sample stage are sequentially irradiated, at least in the main scanning direction, a scanning mechanism for relatively moving, and emitted from the excitation light source, by the irradiation optical system, A plurality of dot-shaped photostimulable phosphor layer regions are excited by the excitation light directed to the sample stage, and the emitted photostimulable light is photoelectrically detected to generate an analog signal. Since it has a detector and an A / D converter that digitizes the analog signal detected by the photodetector to generate a digital signal, it can specifically bind to a substance of biological origin and has a base sequence. The length of the base, the length of the base, the specific binding substance, etc. to the specific binding substance, a substance of biological origin labeled with a radiolabeling substance is specifically bound by hybridization or the like, and a selectively labeled spot is obtained. Even when formed in high density on the surface of a carrier such as a membrane filter, a plurality of dot-shaped photostimulable phosphor layer regions are formed on the support in the same pattern as the spots formed on the surface of the carrier such as a membrane filter. By forming a membrane filter, etc. and a stimulable phosphor sheet by forming them, when exposing, the radioactive labeling substance contained in each spot Emitted electron beam, in the dot-shaped stimulable phosphor layer region other than the dot-shaped stimulable phosphor layer region to be exposed by the electron beam emitted from the radioactive labeling substance contained in the spot Can be reliably prevented from entering and therefore
By scanning a plurality of dot-shaped photostimulable phosphor layer region selectively exposed by excitation light, by photoelectrically detecting the photostimulable light emitted from the dot-shaped photostimulable phosphor layer region , It is possible to generate data for biochemical analysis with high resolution and high quantitativeness.

In a preferred embodiment of the present invention, the scanner further includes a relative positional relationship in the main scanning direction between the excitation control means for controlling the excitation light source and the scanning mechanism, the irradiation optical system and the sample stage. Position detection means for detecting, the excitation control means, based on the relative positional relationship in the main scanning direction of the irradiation optical system and the sample stage detected by the position detection means, the plurality of Energy per unit area of the excitation light irradiated to the dot-shaped stimulable phosphor layer region, as compared with the region other than the plurality of dot-shaped stimulable phosphor layer region, to be higher, It is configured to control the excitation light source and the scanning mechanism.

According to a preferred embodiment of the present invention, the scanner further detects the relative positional relationship between the excitation control means for controlling the excitation light source and the scanning mechanism, the irradiation optical system and the sample stage in the main scanning direction. Position detection means, the excitation control means, based on the relative positional relationship in the main scanning direction between the irradiation optical system and the sample stage detected by the position detection means, a plurality of dot-shaped stimulable phosphors The excitation light source and the scanning mechanism are controlled so that the energy per unit area of the excitation light with which the layer region is irradiated becomes higher than the regions other than the plurality of dot-shaped stimulable phosphor layer regions. Therefore, with the scanning of the excitation light, the adjacent dot-shaped stimulable phosphor layer regions to be excited next are excited, emit the stimulable light, and are accumulated. To release radiation energy can be reliably prevented, thus, as desired, it is possible to produce biochemical analysis data having an excellent quantitative characteristic.

In a preferred embodiment of the present invention, the scanning mechanism relatively moves the irradiation optical system and the sample stage in the main scanning direction and a sub scanning direction orthogonal to the main scanning direction. Is configured.

According to a preferred embodiment of the present invention, the scanning mechanism is configured to relatively move the irradiation optical system and the sample stage in the main scanning direction and the sub scanning direction orthogonal to the main scanning direction. Therefore, it becomes possible to efficiently generate the biochemical analysis data.

In a preferred embodiment of the present invention, the excitation control means performs the scanning so as to move the irradiation optical system and the sample stage relatively and intermittently in the main scanning direction. While controlling the mechanism, in each of the plurality of dot-shaped stimulable phosphor layer region,
It is configured to control the excitation light source so that the excitation light is emitted for a predetermined time.

According to a preferred embodiment of the present invention, the excitation control means controls the scanning mechanism so as to move the irradiation optical system and the sample stage relatively and intermittently in the main scanning direction. In addition, the plurality of dot-shaped stimulable phosphor layer regions are respectively configured to control the excitation light source so that the excitation light is irradiated for a predetermined time. As a result, the adjacent dot-shaped photostimulable phosphor layer regions to be excited next are surely prevented from being excited and emitting photostimulable light to release accumulated radiation energy. Therefore, it becomes possible to generate biochemical analysis data with excellent quantitativeness as desired.

In a further preferred aspect of the present invention, the excitation control means performs the excitation while the irradiation optical system and the sample stage are relatively and intermittently moved in the main scanning direction. The excitation light source is controlled so that light is constantly applied to the stimulable phosphor sheet.

According to a further preferred embodiment of the present invention, the excitation control means controls the excitation light source so that the excitation light is constantly applied to the stimulable phosphor sheet. Since the scanning mechanism is controlled so as to move the irradiation optical system and the sample stage relatively and intermittently in the main scanning direction, the excitation is performed next with the scanning of the excitation light. It is possible to reliably prevent adjacent dot-shaped stimulable phosphor layer regions that should be excited and emit stimulable light to release the accumulated radiation energy. As described above, it becomes possible to generate biochemical analysis data with excellent quantitativeness.

In another preferred aspect of the present invention, the excitation control means irradiates the excitation light only to the plurality of dot-shaped stimulable phosphor layer regions, and the excitation light of the plurality of dot-shaped phosphors is irradiated. The excitation light source is controlled to be turned on / off so that the region other than the exhaustive phosphor layer region is not irradiated with the excitation light.

According to another preferred embodiment of the invention,
Excitation control means, only in the plurality of dot-shaped stimulable phosphor layer region, the excitation light is irradiated, the region other than the plurality of dot-shaped stimulable phosphor layer region, so that the excitation light is not irradiated Since the excitation light source is configured to be turned on and off, only the dot-shaped stimulable phosphor layer region to be excited is irradiated with the excitation light at each time point. Along with the scanning of the excitation light, the adjacent dot-shaped stimulable phosphor layer regions to be excited next are excited and emit the stimulable light to release the accumulated radiation energy. Can be prevented more reliably,
Therefore, it is possible to generate biochemical analysis data with excellent quantitativeness as desired.

In a further preferred aspect of the present invention, the scanning mechanism sets the distance between the irradiation optical system and the sample stage between the dot-shaped stimulable phosphor layer regions adjacent to each other in the main scanning direction. It is configured to move relatively and intermittently in the main scanning direction at a pitch equal to.

In a preferred aspect of the present invention, the scanning mechanism is configured to move the irradiation optical system and the sample stage relatively and continuously in the main scanning direction. The excitation control means is substantially
Only in the plurality of dot-shaped stimulable phosphor layer regions, the excitation light is irradiated, the region other than the plurality of dot-shaped stimulable phosphor layer regions, so that the excitation light is not irradiated, The excitation light source is configured to be turned on / off.

According to a preferred embodiment of the present invention, the scanning mechanism is configured to move the irradiation optical system and the sample stage relatively and continuously in the main scanning direction. Excitation control means, substantially only a plurality of dot-shaped stimulable phosphor layer region, the excitation light is irradiated,
A region other than a plurality of dot-shaped stimulable phosphor layer regions, so that the excitation light is not irradiated, because it is configured to control the on / off of the excitation light source, along with the scanning of the excitation light, Adjacent dot-shaped stimulable phosphor layer regions to be excited next, excited, emits stimulable light, it is possible to reliably prevent the release of accumulated radiation energy, Therefore, it is possible to generate biochemical analysis data with excellent quantitativeness as desired.

In a preferred embodiment of the invention, the scanner further comprises integrating means for integrating the analog signal produced by the photodetector.

According to a preferred embodiment of the present invention, the scanner further comprises an integrating means for integrating the analog signal generated by the photodetector. Exciting the stimulable phosphor contained in the stimulable phosphor layer region, photoelectrically detecting the stimulable light emitted from the stimulable phosphor, integrating the generated analog signal, By digitizing the integrated value of the analog signal and generating biochemical analysis data, the radiation energy accumulated in the dot-shaped photostimulable phosphor layer region is small, and the dot-shaped photostimulation is generated by the excitation light. When the stimulable phosphor contained in the stimulable phosphor layer region is excited, even if the intensity of the stimulable light emitted from the dot-shaped stimulable phosphor layer region is small, good sensitivity, sufficient Digi with very high signal strength It is possible to generate a Rudeta.

In a preferred embodiment of the present invention, the scanner further comprises addition means for adding the digital signals generated by the A / D converter.

According to a preferred embodiment of the present invention, since the scanner further comprises an adding means for adding the digital signals generated by the A / D converter, the scanner is irradiated with the excitation light to emit a plurality of signals. Exciting the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region, photoelectrically detecting the stimulable light emitted from the stimulable phosphor, the generated analog signal By digitizing and adding the generated digital signals to generate biochemical analysis data, the radiation energy accumulated in the dot-shaped stimulable phosphor layer region is small, and the dot shape is generated by the excitation light. When the stimulable phosphor contained in the stimulable phosphor layer region is excited, even if the intensity of the stimulable light emitted from the dot-shaped stimulable phosphor layer region is small, the sensitivity is high. Well, give a big enough signal strength It becomes possible to generate a digital data.

In a preferred aspect of the present invention, the scanning mechanism is configured to move the sample stage in the main scanning direction.

In a further preferred aspect of the present invention, the position detecting means is constituted by a linear encoder for detecting the position of the sample stage in the main scanning direction.

In a further preferred aspect of the present invention, the scanning mechanism includes a stepping motor which intermittently moves the sample stage in the main scanning direction.

In a further preferred aspect of the present invention, the position detecting means is constituted by a rotary encoder for detecting the rotational position of the stepping motor.

In another preferred aspect of the present invention, the scanning mechanism is configured to move the irradiation optical system in the main scanning direction.

In a further preferred aspect of the present invention, the position detecting means is constituted by a linear encoder for detecting the position of the irradiation optical system in the main scanning direction.

In a further preferred aspect of the present invention, the scanning mechanism includes a stepping motor which intermittently moves the irradiation optical system in the main scanning direction.

In a further preferred aspect of the present invention, the position detecting means is constituted by a rotary encoder for detecting the rotational position of the stepping motor.

The stimulable phosphor contained in the stimulable phosphor layer used in the present invention is capable of accumulating radiation energy, is excited by electromagnetic waves, and accumulates the accumulated energy in the form of light. It is not particularly limited as long as it can be emitted by the above, but it is preferable that it can be excited by light in the visible light wavelength range. Specifically, for example, the alkaline earth metal fluorohalide-based phosphor (B shown in US Pat. No. 4,239,968) is disclosed.
a1-xM ²⁺ x) FX: yA (where M ²⁺ is M
at least one alkaline earth metal element selected from the group consisting of g, Ca, Sr, Zn and Cd, X is Cl,
At least one halogen selected from the group consisting of Br and I, A is Eu, Tb, Ce, Tm, Dy, Pr,
At least one trivalent metal element selected from the group consisting of Ho, Nd, Yb and Er, x is 0 ≦ x ≦ 0.6, y
Is 0 ≦ y ≦ 0.2. ), JP-A-2-276997
Alkaline earth metal fluoride halide phosphor SrFX: Z (where X is at least one halogen selected from the group consisting of Cl, Br and I,
Z is Eu or Ce. ), JP-A-59-5647
Europium-activated composite halogen-based phosphor BaFX · xNaX ′: aEu ²⁺ (disclosed here)
Both X and X ′ are at least one halogen selected from the group consisting of Cl, Br and I, x is 0 <x ≦ 2, and a is 0 <a ≦ 0.2. ), JP-A-5
MOX: xCe (here, M is Pr, Nd, Pm, Sm, Eu, Tb, D) which is a cerium-activated trivalent metal oxyhalogen-based phosphor disclosed in Japanese Unexamined Patent Publication No. 8-69281.
at least one trivalent metal element selected from the group consisting of y, Ho, Er, Tm, Yb and Bi, X is one or both of Br and I, and x is 0 <x <0.1
Is. ), LnOX: xCe, which is a cerium-activated rare earth oxyhalogen-based phosphor disclosed in U.S. Pat. No. 4,539,137 (wherein Ln is at least selected from the group consisting of Y, La, Gd and Lu). One rare earth element, X is at least one halogen selected from the group consisting of Cl, Br and I, x is 0 <x ≦ 0.1) and US Pat. No. 4,962,047. Europium-activated composite halide phosphor M ^II F
X ・ aM ^I X '・ bM' ^II X ^" 2 ・ cM ^III X ^'" 3 ・
xA: yEu ²⁺ (where M ^II is Ba, Sr and C
at least one alkaline earth metal element selected from the group consisting of a, M ^I is Li, Na, K, Rb and Cs
At least one alkali metal element selected from the group consisting of, M ′ ^II is at least one divalent metal element selected from the group consisting of Be and Mg, M ^III is Al, Ga,
At least one trivalent metal element selected from the group consisting of In and Tl, A is at least one metal oxide, X
Is at least one halogen selected from the group consisting of Cl, Br and I, and X ′, X ^″ and X ^{′ ″} are F and C.
is at least one halogen selected from the group consisting of 1, Br and I, a is 0 ≦ a ≦ 2, b is 0 ≦ b
≦ 10 ⁻² , c is 0 ≦ c ≦ 10 ⁻² , and a + b
+ C ≧ 10 ⁻² , x is 0 <x ≦ 0.5, and y
Is 0 <y ≦ 0.2. ) Can be preferably used.

[0099]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit.

As shown in FIG. 1, the biochemical analysis unit 1 has a large number of substantially circular through holes 3 formed of a flexible metal having a property of attenuating radiation.
Is provided with a substrate 2 formed in high density, and a large number of through holes 3 are filled with nylon 6 capable of forming a membrane filter to form a large number of dot-shaped absorptive regions 4. There is.

Although not shown exactly in FIG. 1, in the present embodiment, the through holes 3 having a diameter of about 0.01 square millimeters of about 10,000 are regularly arranged at a density of about 5000 holes / square centimeter. Formed on the substrate 2. The adsorptive region 4 is formed by filling a large number of through holes 3 with a porous material so that the surface thereof is located below the surface of the substrate 2.

FIG. 2 is a schematic front view of the spotting device.

As shown in FIG. 2, the spotting device 5 is provided with an injector 6 and a CCD camera 7, and CC
With the D camera 7, the tip of the injector 6 and the cD
While observing the through hole 3 of the biochemical analysis unit 1 in which the specific binding substance such as NA should be dropped, the tip of the injector 6 and the center of the through hole 3 in which the specific binding substance should be dropped match. Sometimes, the specific binding substance is configured to be dropped from the injector 6, and the specific binding substance is accurately dropped into the large number of dot-shaped absorptive regions 4 of the biochemical analysis unit 1. Is guaranteed to be.

FIG. 3 is a schematic cross-sectional view of the hybridization container.

As shown in FIG. 3, the hybridization container 8 has a cylindrical shape, and a hybridization liquid 9 containing a substance of biological origin labeled with a labeling substance is contained therein.

In this embodiment, a hybridizing liquid 9 containing a substance of biological origin labeled with a radioactive labeling substance is prepared and housed in a hybridization container 8.

On the other hand, with a fluorescent substance such as a fluorescent dye,
In the case of selectively labeling a specific binding substance such as cDNA, a hybridizing liquid 9 containing a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye is prepared and housed in the hybridization container 8. .

During hybridization, the biochemical analysis unit 1 in which a specific binding substance such as cDNA is dripped into a large number of dot-shaped absorptive regions 4 is inserted into the hybridization container 8, but Since 2 is formed of a metal, as shown in FIG. 3, the biochemical analysis unit 1 should be curved and inserted into the hybridization container 8 along the inner wall of the hybridization container 8. You can

As shown in FIG. 3, the hybridization container 8 is configured to be rotatable about its axis by driving means (not shown), and the biochemical analysis unit 1 is in a curved state. Since it is inserted into the hybridizing container 8 along the inner wall of the, the hybridizing container 8 is rotated so that even if the amount of the hybridizing liquid 9 is small, a large number of dot-shaped absorptive regions 4 are formed. The dropped specific binding substance such as cDNA was labeled with a radioactive labeling substance, and was labeled with a biological substance contained in the hybridizing liquid 9 or a fluorescent substance such as a fluorescent dye, and contained in the hybridizing liquid 9. The substance derived from the living body is selectively hybridized.

FIG. 4 is a schematic perspective view of a stimulable phosphor sheet according to a preferred embodiment of the present invention.

As shown in FIG. 4, the stimulable phosphor sheet 10 according to the present embodiment has a support 11 made of oxygen-free copper and a large number of recesses 13 formed on the support 11, which are bright. A large number of dot-shaped stimulable phosphor layer regions 12 are formed by embedding a stimulable phosphor. In this embodiment, the dot-shaped stimulable phosphor layer region 1
The stimulable phosphor is embedded in a large number of recesses 13 so that the surface of 2 is located above the surface of the support 11.

Here, the large number of recesses 13 has the same regular pattern as the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1, and therefore the large number of dot-shaped absorptive regions 4. In addition, the support 11 is formed in a substantially circular shape having the same size.

In FIG. 5, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are exposed by the radioactive labeling substance contained in the large number of dot-shaped absorptive regions 4. It is a schematic sectional drawing which shows the method of doing.

As shown in FIG. 5, upon exposure, a large number of stimulable phosphors are embedded in a large number of recesses 13 formed in the support 11 of the stimulable phosphor sheet 10. The dot-shaped stimulable phosphor layer regions 12 are respectively located in a large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1, and the absorptive regions 4 in the through holes 3 are located.
The stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 1 so as to face the.

Upon exposure, an electron beam is emitted from the radioactive labeling substance contained in the large number of dot-shaped absorptive regions 4, but the substrate 2 of the biochemical analysis unit 1 is formed of a metal that attenuates radiation. Therefore, the electron beam emitted from the radiolabeled substance is reliably prevented from being scattered in the substrate 2, and a large number of dot-shaped stimulable phosphor layer regions 12 are provided in the biochemical analysis unit 1. A large number of through holes 3 formed in the substrate 2, that is, a large number of dot-shaped absorptive regions 4 and the same regular pattern are formed on the support body 11, and the substrates of the biochemical analysis unit 1 are respectively formed. Two
Since the stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 1 so as to be located in the large number of through holes 3 formed in, the adsorptivity in each through hole 3 formed in the substrate 2 The electron beam emitted from the radioactive labeling substance contained in the region 4 is incident only on the dot-shaped stimulable phosphor layer region 12 which is opposed thereto, and the support 11 is formed of stainless steel which attenuates the radiation. Therefore, the electron beam is also prevented from being scattered in the support 11 of the stimulable phosphor sheet 10, and therefore the radioactive radiation contained in the absorptive region 4 in each through hole 3 formed in the substrate 2 is prevented. Only the corresponding dot-shaped stimulable phosphor layer region 12 can be reliably exposed by the labeling substance.

In this way, the biochemical analysis data of the radioactive labeling substance is recorded in the dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10.

FIG. 6 shows the digital data for biochemical analysis by reading the radiation data of the radiolabeled substance recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10. FIG. 8 is a schematic perspective view showing an example of a scanner that generates data, and FIG. 7 is a schematic perspective view showing details of the vicinity of the photomultiplier.

The scanner shown in FIG. 6 is a unit for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10. Of a fluorescent substance such as a fluorescent dye labeling a substance derived from a living body hybridized to a specific binding substance such as cDNA contained in a large number of dot-shaped absorptive regions 4 formed on the substrate 2 of 1 It is configured to read fluorescence data and generate digital data for biochemical analysis. The first laser excitation light source 21 emits laser light 24 having a wavelength of 640 nm, and 532 nm.
Second laser excitation light source 2 for emitting laser light 24 of
2 and a third laser excitation light source 23 that emits laser light 24 having a wavelength of 473 nm.

In this embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source,
Laser excitation light source 22 and third laser excitation light source 23
Is composed of a second harmonic generation element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. First
In the optical path of the laser light 24 emitted from the laser excitation light source 21 and reflected by the mirror 26, the first dichroic mirror 27 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer A second dichroic mirror 28 that transmits light of a wavelength and reflects light of a wavelength of 473 nm is provided.
Laser light 24 generated by the laser excitation light source 21 of
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated from the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, it is reflected by the first dichroic mirror 27, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 28 and enters the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser light 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 or the biochemical analysis unit 1 placed on 41. When setting the biochemical analysis unit 1 on the stage 40, the biochemical analysis unit 1 is set so that the surface of the adsorptive region 4 on which the specific binding substance such as cDNA is dropped faces downward. It is placed on the glass plate 41 of 40.

Laser light 24 is applied to the stimulable phosphor sheet 10.
Is incident, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are excited, stimulable light 45 is emitted, and the biochemical analysis unit is also used. When the laser light 24 is incident on the laser light 1, the fluorescent substance such as the fluorescent dye contained in the absorptive region 4 in the large number of through holes 3 is excited and the fluorescence 45 is emitted.

Photostimulable light 45 emitted from a large number of dot-shaped stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 or absorptive regions 4 in a large number of through holes 3 of the biochemical analysis unit 1. The fluorescent light 45 emitted from the optical head 35
Is focused on a mirror 36 by an aspherical lens 37 provided on the optical axis, and is reflected by the mirror 36 on the same side as the optical path of the laser light 24 to be parallel light.
Incident on.

The photostimulable light 45 or the fluorescent light 45 incident on the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror and enters the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 7, the filter unit 48 includes four filter members 51a, 51b, 51c,
51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 7 by a motor (not shown).

FIG. 8 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 8, the filter member 51
a includes a filter 52a, and the filter 52a uses the first laser excitation light source 21 and uses the biochemical analysis unit 1
Is a filter member that is used when fluorescence is read by exciting a fluorescent substance such as a fluorescent dye contained in 6
It has a property of cutting light having a wavelength of 40 nm and transmitting light having a wavelength longer than 640 nm.

FIG. 9 is a sectional view taken along the line BB of FIG.

As shown in FIG. 9, the filter member 51
b includes a filter 52b, and the filter 52b uses the second laser excitation light source 22 and uses the biochemical analysis unit 1
53 is a filter member used when exciting a fluorescent substance such as a fluorescent dye contained in to read fluorescence.
It has a property of cutting light having a wavelength of 2 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 10 is a sectional view taken along the line CC of FIG.

As shown in FIG. 10, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
Is a filter member used when exciting a fluorescent substance such as a fluorescent dye contained in the biochemical analysis unit 1 by using the laser excitation light source 23 of FIG.
It has a property of cutting light having a wavelength of 73 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 11 is a sectional view taken along the line DD of FIG.

As shown in FIG. 11, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
A large number of dot-shaped stimulable phosphor layer regions 1
2 is a filter used when exciting 2 and reading the stimulable light 45 emitted from the stimulable phosphor layer region 12,
It has a property of transmitting only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 and cutting the light of the wavelength of 640 nm.

Therefore, depending on the laser excitation light source to be used, the filter members 51a, 51b, 51c, 51d.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

Although not shown in FIG. 6, the optical head 35 is configured to be movable in the X direction and the Y direction in FIG. 6 by the scanning mechanism, and the entire surface of the biochemical analysis unit 1 is laser light. It is configured to be scanned by 24.

FIG. 12 is a schematic plan view of the scanning mechanism of the optical head. In FIG. 12, for simplification, the optical system except the optical head 15, the laser light 24, and the fluorescent light 4 are shown.
The optical path of 5 or stimulated emission 45 is omitted.

As shown in FIG. 12, the optical head 35
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
Are fixed, and a substrate 63 which is movable in the sub-scanning direction indicated by Y in FIG. 12 is further provided on the substrate 60.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in this hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63, and the main scanning stepping motor 65 connects the endless belt 66 to the adjacent through holes 3 formed in the biochemical analysis unit 1. That is, it is configured such that it can be driven intermittently at a pitch equal to the distance of the absorptive region 4. The optical head 35 is fixed to the endless belt 66, and the main scanning stepping motor 6
5, when the endless belt 66 is driven, the endless belt 66 is moved in the main scanning direction indicated by X in FIG. In FIG. 12, 67 is a linear encoder that detects the position of the optical head 35 in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is intermittently driven in the main scanning direction, and when scanning of one line is completed, the substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61. The optical head 35 is
In FIG. 12, the laser beam 24 is moved in the XY direction.
Thus, the entire surface of the biochemical analysis unit 1 or the stimulable phosphor sheet 10 is scanned.

FIG. 13 is a block diagram showing the control system, input system, drive system and detection system of the scanner shown in FIG.

As shown in FIG. 13, the control system of the scanner is a control unit 7 for controlling the entire scanner.
0, a data processing device 54, and an input system of the scanner, which is operated by an operator and has a keyboard 71 capable of inputting various instruction signals.

As shown in FIG. 13, the drive system of the scanner has a main scanning stepping motor 65 for intermittently moving the optical head 35 in the main scanning direction and an intermittent movement of the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

Further, as shown in FIG. 13, the detection system of the scanner comprises a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser pumping light source 21, the second laser pumping light source 22 or the third laser pumping light source 21 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The scanner according to the present embodiment having the above-described structure is constructed as follows, and the radioactive label contained in the large number of dot-shaped absorptive regions 4 formed in the biochemical analysis unit 1 is as follows. A large number of dot-shaped photostimulable phosphor layer regions 12 are exposed by the substance, and the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 10 is read to generate digital data for biochemical analysis. To do.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40 so that a large number of dot-shaped photostimulable phosphor layer regions 12 are in contact with the surface of the glass plate 41.

[0157] Next, the user operates the keyboard 7
An instruction signal for scanning a large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 with a laser beam 24 is input to the display device 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
The filter member 51d provided with the filter 52d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is positioned in the optical path of the stimulated emission light 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, When it is confirmed that the optical head 35 has reached one of the dot-shaped stimulable phosphor layer regions 12 at a position where the laser beam 24 can be irradiated, a stop signal is output to the main scanning stepping motor 65. Together with the first laser excitation light source 2
A drive signal is output to 1 to activate the first laser excitation light source 21 to emit laser light 24 having a wavelength of 640 nm.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser beam 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36, and by the aspherical lens 37, the dot-shaped stimulable phosphor layer region 1 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41.
It is focused on one of the two.

As a result, the stimulable phosphor contained in one of the dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 is excited by the laser beam 24 and stimulated. The photostimulable light 45 is emitted from one of the luminescent phosphor layer regions 12.

The photostimulable light 45 emitted from one of the dot-shaped photostimulable phosphor regions 12 is condensed by the aspherical lens 37 provided in the optical head 35, and the laser light 24 of the laser light 24 is reflected by the mirror 36. The light is reflected on the same side as the optical path, becomes parallel light, and enters the perforated mirror 34.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and enters the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected by the photomultiplier 50 and generated is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

After the first laser excitation light source 21 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21, While driving the first laser excitation light source 21 is stopped, a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move to the adjacent dots formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor layer regions 12 are moved by a pitch equal to the distance between the regions.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is confirmed to have been moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12, the control unit 70 outputs a drive signal to the first laser excitation light source 21. Then, the first laser excitation light source 2
1 is turned on, and the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser beam 24.

Similarly, the laser beam 24 is applied to the second dot-shaped stimulable phosphor layer area 12 formed on the stimulable phosphor sheet 10 for a predetermined time, and the second stimulable phosphor layer 12 is irradiated. The stimulated emission 45 emitted from the luminescent phosphor layer region 12 is
When photoelectrically detected by the photomultiplier 50, the control unit 70 outputs an off signal to the first laser excitation light source 21 to turn off the first laser excitation light source 21, and at the same time, to perform the main scanning stepping motor. A drive signal is output to 65 to move the optical head 35 by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12.

In this way, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal of the optical head 35 input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser light 24 is completed, the control unit Reference numeral 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position, and also outputs a drive signal to the sub scanning pulse motor 61 to scan the movable substrate 63 in the sub scanning direction. Move one line in the direction.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line is moved by one line in the sub-scanning direction, the control unit 70 sequentially applies the first laser excitation to the dot-shaped stimulable phosphor layer region 12 of the first line. The laser light emitted from the first laser excitation light source 21 is sequentially applied to the dot-shaped stimulable phosphor layer region 12 of the second line in the same manner as the irradiation of the laser light 24 emitted from the light source 21. 24 to excite the dot-shaped stimulable phosphor layer region 12, and stimulated the photostimulable light 45 emitted from the stimulable phosphor layer region 12 into a photomultiplier 50 sequentially photoelectrically. Let it be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

In this way, all the dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are scanned by the laser light 24 emitted from the first laser excitation light source 21, and a large number of them are scanned. The dot-shaped photostimulable phosphor layer region 12 is excited, and the emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into the A / D converter 53. Due to
When converted into digital data and sent to the data processing device 54, a drive stop signal is output from the control unit 70 to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped. .

On the other hand, a substance derived from a living body which is hybridized with a specific binding substance such as cDNA contained in a large number of dot-shaped absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is labeled. In the case of reading fluorescence data of a fluorescent substance such as a fluorescent dye and generating data for biochemical analysis, a hybridizing liquid 9 containing a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye is prepared. Then, the biochemical analysis unit 1 is accommodated in the hybridization container 8, and the biochemical analysis unit 1 is inserted into the hybridization container 8 along the inner wall of the hybridization container 8 in a curved state. A specific binding substance such as cDNA dropped in a large number of dot-shaped absorptive regions 4 is labeled with a fluorescent substance such as a fluorescent dye and contained in the hybridization liquid 9. Substance derived from a living organism is, selectively,
Hybridized.

After that, the biochemical analysis unit 1 is placed on the glass plate 41 of the stage 40 so that the absorptive region 4 is located on the glass plate 41 side.

[0180] Next, the user operates the keyboard 7
An instruction signal for identifying a fluorescent dye or the like labeling a substance of biological origin is input to the control unit 70.
Thus, from the first laser excitation light source 21, the second laser excitation light source 22 and the third laser excitation light source 23, a wavelength that can efficiently excite the fluorescent substance labeling the substance of biological origin. The laser excitation light source that emits the laser light 24 of
Among a, 51b, 51c, a filter member having a property of cutting light having a wavelength of the laser light 24 used to excite the fluorescent substance and transmitting light having a wavelength longer than the wavelength of the excitation light is selected. Similarly to the case of the stimulable phosphor sheet 10, the laser light 24 scans the biochemical analysis unit 1 and the fluorescence 45 emitted from the fluorescent dye.
Is photoelectrically detected by the photomultiplier 50 to generate analog data, and digitized by the A / D converter to generate digital data for biochemical analysis.

According to this embodiment, when a large number of dot-shaped stimulable phosphor layer regions 12 are exposed by the radioactive labeling substance contained in a large number of dot-shaped absorptive regions 4, a large number of dot-shaped stimulable phosphor layer regions 12 are exposed. A high-energy electron beam is emitted from the radiolabeled substance contained in the absorptive region 4, but since the substrate 2 of the biochemical analysis unit 1 is made of a metal that attenuates radiation, it is emitted from the radiolabeled substance. The scattered electron beam is surely prevented from being scattered in the substrate 2, and a large number of dot-shaped stimulable phosphor layer regions 12 are formed on the substrate 2 of the biochemical analysis unit 1. The through holes 3 and the S-dot-shaped absorptive regions 4 are formed on the support 11 in the same regular pattern, and are formed in the many through holes 3 of the substrate 2 of the biochemical analysis unit 1, respectively. Adsorbable area 4 As opposed, the stimulable because phosphor sheet 10 is superposed on the biochemical analysis unit 1, the absorptive regions 4 of each through-hole 3 formed in the substrate 2
The electron beam emitted from the radiolabeled substance contained in is incident only on the corresponding dot-shaped stimulable phosphor layer region 12, and further, the support 11 is formed by oxygen-free copper that attenuates the radiation. Therefore, the electron beam is also prevented from being scattered in the support 11 of the stimulable phosphor sheet 10, and therefore, the radioactivity contained in the absorptive region 4 in each through hole 3 formed in the substrate 2 is prevented. Since it is possible to reliably expose only the corresponding dot-shaped stimulable phosphor layer region 12 by the labeling substance, the radioactive labeling substance contained in the absorptive region 4 in each through hole 3 is used for the exposure. The region 12 of the stimulable phosphor layer to be exposed is exposed to the electron beam emitted from the radiolabel substance contained in the adsorptive region 4 in the adjacent through hole 3 to generate noise due to exposure. Data for chemical analysis Be generated can be prevented in, it is possible to greatly improve the quantitative characteristic of the biochemical analysis.

Furthermore, according to this embodiment, since the support 11 of the stimulable phosphor sheet 10 is made of oxygen-free copper that attenuates radiation, the dot shape of the stimulable phosphor sheet 10 is exposed at the time of exposure. Stimulable phosphor layer region 12 of
It is possible to reliably prevent external light from entering, and therefore the radioactive label contained in the absorptive region 4 in the through hole 3 facing the substrate 2 of the biochemical analysis unit 1 is included. Radiation energy accumulated in the dot-shaped stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 in response to the irradiation of the electron beam emitted from the substance is released by the incidence of external light. Since it is possible to reliably prevent this, it is possible to significantly improve the quantitativeness of biochemical analysis.

Even if the support 11 is formed of a material that attenuates radiation, when the support emits radiation when the support is irradiated with the electron beam emitted from the radioactive labeling substance, A large number of dot-shaped stimulable phosphor layer regions are exposed by the radiation emitted from the support to generate fog. According to the present embodiment, the support 11 of the stimulable phosphor sheet 10 is Attenuating radiation,
Since it is formed of oxygen-free copper that does not substantially emit radiation even when irradiated with radiation, the support 11 is also supported when the electron beam emitted from the radiolabeled substance is applied to the support 11. No radiation is emitted from 11 and, therefore, when the electron beam emitted from the radio-labeled substance is irradiated, the fogging caused by the emission of radiation from the support 11 is effectively generated. It becomes possible to prevent.

Further, in this embodiment, the optical head 35 is moved in the main scanning direction by the main scanning stepping motor 65, and the dot-shaped dot signal is generated based on the position detection signal of the optical head 35 input from the linear encoder 67. When it is confirmed that the optical head 35 has reached a position where the laser light 24 can be irradiated to one of the stimulable phosphor layer regions 12, the first laser excitation light source 21 is activated, and 64
A laser beam 24 having a wavelength of 0 nm is applied to one of the dot-shaped stimulable phosphor layer regions 12 to excite the stimulable phosphor contained in the dot-shaped stimulable phosphor layer regions 12. Then, the emitted photostimulated light 45 is photoelectrically detected by the photomultiplier 50.

After the first laser excitation light source 21 is driven, a predetermined time, for example, several μ seconds, elapses, the first laser excitation light source 21 is driven.
The driving of the laser excitation light source 21 of the
5 is moved by a pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is confirmed to have been moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12, the first laser excitation light source 21 is activated, and 640n
The second dot-shaped photostimulable phosphor layer region 12 adjacent to the first dot-shaped photostimulable phosphor layer region 12 is irradiated with the laser light 24 having a wavelength of m to obtain the second dot-shaped photostimulable phosphor layer region 12. Of the stimulable phosphor contained in the stimulable phosphor layer region 12 of
The emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50.

When a predetermined time has passed after the first laser excitation light source 21 was turned on, the first laser excitation light source 2
1 is stopped, and the optical head 35 is moved by a pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. .

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 input from the linear encoder 67. , Optical head 35
Is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser light 24 is completed, the optical head 35 Is returned to its original position, and the movable scanning substrate 63 is moved by the sub-scanning pulse motor 61 by one line in the sub-scanning direction, and the dot-shaped stimulable phosphor of the first line is moved. Just as the layer region 12 was successively irradiated with the laser light 24 emitted from the first laser excitation light source 21, the dot-shaped stimulable phosphor layer region 12 of the second line was sequentially irradiated. The laser light 24 emitted from the first laser excitation light source 21 is irradiated to excite the dot-shaped photostimulable phosphor layer region 12, and the emitted photostimulable light 45 is sequentially emitted by the photomultiplier 50. , Photoelectrically detected .

In this way, the large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are all scanned by the laser light 24 emitted from the first laser excitation light source 21, and a large number of them are scanned. The dot-shaped photostimulable phosphor layer region 12 is excited, and the emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into the A / D converter 53. Is converted into digital data by the data processing device 5
4, the driving of the first laser excitation light source 21 is stopped, and the generation of biochemical analysis data is completed.

Therefore, according to this embodiment, the optical head 35 is moved intermittently, and the optical head 35 is moved to a position where only the dot-shaped stimulable phosphor layer region 12 can be irradiated with the laser light 24. When it reaches, the optical head 35 is stopped, and one of the dot-shaped stimulable phosphor layer regions 12 is irradiated with the laser light 24 from the first laser excitation light source 21 for a predetermined time, and the predetermined laser light is emitted. After a lapse of time, the driving of the first laser excitation light source 21 is stopped, and the optical head 35 is positioned at a position where the laser beam 24 can be irradiated only to the adjacent dot-shaped stimulable phosphor layer regions 12. The dot-shaped stimulable phosphor layer regions 12 adjacent to each other are moved from the first laser excitation light source 21 for a predetermined time.
In addition, since it is configured to be irradiated with laser light,
As the laser light 24 is scanned, the laser light 2 is applied to the adjacent dot-shaped photostimulable phosphor layer regions 12 to be excited next.
4 is irradiated, the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 is excited, and the accumulated radiation energy is emitted in the form of stimulable light 45. This can be surely prevented, and therefore, it becomes possible to generate data for biochemical analysis that is highly quantitative.

FIG. 14 is a block diagram around the photomultiplier 50 and the data processor 54 of the scanner according to another preferred embodiment of the present invention.

As shown in FIG. 14, the scanner according to this embodiment includes an integrating amplifier 75 for integrating the analog signal generated by the photomultiplier 50, and the integrated value of the analog signal generated by the integrating amplifier 75. Are digitized by the A / D converter 53 and stored in the data memory 76.

In this embodiment, one of the dot-shaped stimulable phosphor layer regions 12 is irradiated with the laser beam 24,
The stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 was excited, and the emitted stimulable light 45 was photoelectrically detected and generated by the photomultiplier 50. The analog signal is integrated by the integrating amplifier 75.

When a predetermined time, for example, several microseconds has elapsed since the first laser pumping light source 21 was started, the control unit 70 outputs a drive stop signal to the first laser pumping light source 21, The driving of the first laser excitation light source 21 is stopped, the analog signal integrated by the integrating amplifier 75 is output to the A / D converter 53, and the integrated value of the analog signal is digitalized by the A / D converter 53. Then, the data is converted into digital data of the dot-shaped stimulable phosphor layer region 12 and output to the data memory 76 for storage.

At the same time, the control unit 70 outputs a drive signal to the main scanning stepping motor 65,
The optical head 35 is attached to the support 1 of the stimulable phosphor sheet 10.
Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35 is moved by a pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 formed in 1. Is confirmed to have been moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12, the control unit 70 outputs a drive signal to the first laser excitation light source 21. Then, the first laser excitation light source 21 is activated, and the laser light 24 excites the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10.

According to the present embodiment, one of the dot-shaped stimulable phosphor layer regions 12 is irradiated with the laser beam 24, and the luminescent material contained in the dot-shaped stimulable phosphor layer regions 12 is irradiated. The photostimulable phosphor 45 is excited and the emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50. An analog signal generated by the photomultiplier 50 is integrated by an integrating amplifier 75, and an integrated value of the analog signal is calculated. , A / D converter 53 digitizes and produces digital data of each dot-shaped stimulable phosphor layer region 12, and therefore is accumulated in the dot-shaped stimulable phosphor layer region 12. The radiation energy is small and the dot-shaped stimulable phosphor layer region 12
Even if the intensity of the photostimulable light 45 emitted from the device is low, it is possible to generate digital data with high sensitivity and sufficiently high signal intensity.

FIG. 15 is a block diagram around the photomultiplier 50 and the data processor 54 of the scanner according to another preferred embodiment of the present invention.

As shown in FIG. 15, the scanner according to the present embodiment has an adding means 77 for adding the digital signals digitized by the A / D converter 53.
A data memory 78 for storing the digital signals added by the adding means 77 is provided.

In this embodiment, the control unit 70 outputs a drive signal to the first laser excitation light source 21, and at the same time, the addition means 7 of the data processing device 54.
7 is configured to output an addition processing execution signal, and one of the dot-shaped photostimulable phosphor layer regions 12 is irradiated with the laser beam 24 to form a dot-shaped photostimulable phosphor layer region. The stimulable phosphor contained in 12 is excited, and the emitted stimulable light 45 is emitted by the photomultiplier 50.
The digital signals photoelectrically detected and digitized by the A / D converter 53 are added by the adding means 77 of the data processing device 54 and stored in the data memory 78.

When a predetermined time, for example, several microseconds has passed since the first laser pumping light source 21 was started, the control unit 70 outputs a drive stop signal to the first laser pumping light source 21, The driving of the first laser excitation light source 21 is stopped, and an addition processing completion signal is output to the addition means 77 of the data processing device 54.

When the addition processing completion signal is input from the control unit 70, the addition means 77 of the data processing device 54 adds the addition value of the digital signal stored in the data memory up to that point, and outputs the addition result as a dot shape. The digital data of the stimulable phosphor layer region 12 is stored in a predetermined memory region of the data memory 78.

At the same time, the control unit 70 outputs a drive signal to the main scanning stepping motor 65,
The optical head 35 is attached to the support 1 of the stimulable phosphor sheet 10.
Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35 is moved by a pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 formed in 1. Is confirmed to have been moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12, the control unit 70 outputs a drive signal to the first laser excitation light source 21. Then, the first laser excitation light source 21 is activated, and the laser light 24 excites the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10.

According to this embodiment, one of the dot-shaped stimulable phosphor layer regions 12 is irradiated with the laser beam 24, and the luminescent material contained in the dot-shaped stimulable phosphor layer regions 12 is irradiated. The photostimulable phosphor 45 excited and emitted is photoelectrically detected by the photomultiplier 50, and A / D
The converter 53 adds the digital signals generated by digitization to generate the digital data of each dot-shaped stimulable phosphor layer area 12. Therefore, the dot-shaped stimulable phosphor layer area is formed. Even if the radiation energy accumulated in 12 is small and the intensity of the photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 is low, it is possible to obtain digital data which is sensitive and has a sufficiently high signal intensity. It will be possible to generate.

FIG. 16 is a block diagram showing a control system, an input system, a drive system and a detection system of a scanner according to another preferred embodiment of the present invention.

As shown in FIG. 16, the drive system of the scanner according to the present embodiment is replaced with the main scanning stepping motor 65 of the scanner shown in FIG.
5 is fixed to the endless belt 66 in the main scanning direction indicated by an arrow Y in FIG.
A main scanning motor 80 that drives continuously is provided.

Also in this embodiment, the control unit 70 controls the first laser excitation light source 21, the second laser excitation light source 22 or the third laser excitation light source 21 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

[0207] The scanner according to the present embodiment configured as described above uses the radioactive labeling substance contained in the large number of spot-shaped regions 3 formed in the biochemical analysis unit 1 as follows. A large number of dot-shaped stimulable phosphor layer regions 12 are exposed to light and the stimulable phosphor sheet 10 is exposed.
The radiographic data of the radiolabeled substance recorded in the above is read to generate digital data for biochemical analysis.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40 so that a large number of dot-shaped photostimulable phosphor layer regions 12 are in contact with the surface of the glass plate 41.

[0209] Next, the user operates the keyboard 7
An instruction signal for scanning a large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 with a laser beam 24 is input to the display device 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
The filter member 51d provided with the filter 52d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is positioned in the optical path of the stimulated emission light 45.

Then, the control unit 70 outputs a drive signal to the main scanning motor 80 to cause the optical head 35 to move.
In the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, the first dot-shaped stimulable phosphor layer region 12 is irradiated with the laser light 24 at a position where it can be irradiated. When it is confirmed that the optical head 35 has reached, a drive signal is output to the first laser excitation light source 21 to activate the first laser excitation light source 21, and 640n
A laser beam 24 having a wavelength of m is emitted.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29 and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

Laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36, and by the aspherical lens 37, the dot-shaped stimulable phosphor layer region 12 formed on the support 11 on the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41. First of all, laser light 2
4 is focused on the first dot-shaped stimulable phosphor layer region 12 to be irradiated.

As a result, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer region 12 is excited by the laser beam 24 to generate the first dot-shaped stimulable phosphor layer. The stimulated emission 45 is emitted from the region 12.

The photostimulable light 45 emitted from the first dot-shaped photostimulable phosphor region 12 is condensed by the aspherical lens 37 provided in the optical head 35, and the laser light 24 is reflected by the mirror 36. The light is reflected on the same side as the optical path, becomes parallel light, and enters the perforated mirror 34.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and enters the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

The main scanning motor 80 is configured to continuously drive the endless belt 66, to which the optical head 35 is fixed, at a constant speed in the main scanning direction indicated by the arrow Y in FIG. Therefore, the optical head 35 is continuously moved in the main scanning direction at a constant speed by the main scanning motor 80, and as a result, the laser light 24 emitted from the first laser excitation light source 21 While continuously moving on the dot-shaped stimulable phosphor layer region 12, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer region 12 is excited.

The control unit 70, based on the position detection signal of the optical head 35 input from the linear encoder 67, emits the laser light 24 emitted from the first laser excitation light source 21 into a first dot-shaped photostimulation. The drive stop signal is output to the first laser excitation light source 21 to drive the first laser excitation light source 21 immediately before the optical head 35 reaches a position where irradiation cannot be performed on the luminescent phosphor layer region 12. Stop.

Here, the speed at which the main scanning motor 80 drives the endless belt 66 is the first laser excitation light source even when the radiation energy accumulated in the dot-shaped stimulable phosphor layer region 12 is small. The laser light 24 emitted from 21 excites the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 while moving on the dot-shaped stimulable phosphor layer region 12. Then, the photomultiplier 50 photoelectrically detects the photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 to generate a digital signal having a sufficient signal intensity. Is set to.

Further, the optical head 35 is moved in the main scanning direction indicated by arrow X in FIG. 6 by the main scanning motor 80, and is controlled based on the position detection signal of the optical head 35 input from the linear encoder. By the unit 70, in the main scanning direction, the second dot-shaped stimulable phosphor layer region 12 adjacent to the first dot-shaped stimulable phosphor layer region 12 and the position where laser light 24 can be irradiated When it is confirmed that the optical head 35 has reached, a drive signal is output to the first laser excitation light source 21,
The first laser excitation light source 21 is activated to emit the laser light 24 having a wavelength of 640 nm.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, then enters the mirror 26, is reflected, and is reflected by the first dichroic mirror 27 and the first dichroic mirror 27. The light passes through the second dichroic mirror 28 and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by mirror 32
4 passes through the hole 33 of the perforated mirror 34, enters the concave mirror 38, is reflected by the concave mirror 38,
It is incident on the optical head 35.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and the aspherical lens 37
Thus, the light is focused on the second dot-shaped stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41.

As a result, the stimulable phosphor contained in the second dot-shaped stimulable phosphor layer region 12 is excited by the laser beam 24 to generate the second dot-shaped stimulable phosphor layer. The photostimulable light 45 is emitted from the region 12, and the photostimulable light 45 emitted from the first dot-shaped photostimulable phosphor layer region 12 is similarly photoelectrically detected by the photomultiplier 50. .

The analog signal photoelectrically detected by the photomultiplier 50 and generated is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

The optical head 3 is driven by the main scanning motor 80.
5 is continuously moved in the main scanning direction at a constant speed,
As a result, the laser light 24 emitted from the first laser excitation light source 21 emits the second dot-shaped stimulable phosphor layer region 1
While continuously moving on 2, the stimulable phosphor contained in the second dot-shaped stimulable phosphor layer region 12 is excited.

The control unit 70, based on the position detection signal of the optical head 35 input from the linear encoder 67, emits the laser light 24 emitted from the first laser excitation light source 21 into a second dot-shaped photostimulation. The drive stop signal is output to the first laser excitation light source 21 to drive the first laser excitation light source 21 immediately before the optical head 35 reaches a position where irradiation cannot be performed on the luminescent phosphor layer region 12. Stop.

In this way, the optical head 35 is continuously moved in the main scanning direction at a constant speed, and the first laser excitation light source 21 is turned on according to the position detection signal of the optical head 35 input from the linear encoder 67. The off-state is repeated, and the dot-shaped stimulable phosphor layer region 12 located on the first line is sequentially irradiated with the laser light 24,
The photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 is photoelectrically detected by the photomultiplier 50 and digitized by the A / D converter 53 to generate a digital signal. Data processing device 54
Sent to.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser beam 24 is completed, the control unit 70 Outputs a drive signal to the main scanning motor 80 to return the optical head 35 to the original position, and outputs a drive signal to the sub scanning pulse motor 61,
The movable substrate 63 is moved by one line in the sub-scanning direction.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line is moved by one line in the sub-scanning direction, the control unit 70 sequentially applies the first laser excitation to the dot-shaped stimulable phosphor layer region 12 of the first line. In the same manner as when the laser light 24 emitted from the light source 21 is irradiated, the first laser excitation light source 2
While controlling ON / OFF of No. 1, the dot-shaped stimulable phosphor layer region 12 of the second line is sequentially irradiated with laser light 24 emitted from the first laser excitation light source 21 to obtain dot-shaped photostimulable phosphor layer regions 12. The stimulable phosphor layer region 12 is excited, and the stimulable light 45 emitted from the stimulable phosphor layer region 12 is sequentially photoelectrically detected by the photomultiplier 50.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

In this way, all the dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are scanned by the laser light 24 emitted from the first laser excitation light source 21, and a large number of them are scanned. The dot-shaped photostimulable phosphor layer region 12 is excited, and the emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into the A / D converter 53. Due to
When converted into digital data and sent to the data processing device 54, a drive stop signal is output from the control unit 70 to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped. .

According to this embodiment, the optical head 35 is
Laser light 24 emitted from the first laser excitation light source 21
Only when the dot-shaped stimulable phosphor layer region 12 can be irradiated with the first laser excitation light source 21 is activated, and the dot-shaped stimulable phosphor layer region 12 is sequentially irradiated. ,
Since the laser light 24 is configured to be irradiated, the laser light 24 is irradiated to the adjacent dot-shaped stimulable phosphor layer regions 12 to be excited next with the scanning of the laser light 24. To surely prevent the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 from being excited and releasing the accumulated radiation energy in the form of stimulable light 45. Therefore, it becomes possible to generate data for biochemical analysis with excellent quantification.

FIG. 17 is a schematic partial sectional view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.

As shown in FIG. 17, the stimulable phosphor sheet 90 according to this embodiment has a support 91 made of silicon nitride, a regular pattern, and a large number of through holes 3. In size, in a substantially circular shape, the support 9
A large number of dot-shaped stimulable phosphor layer regions 92 are formed by filling the inside of the large number of through holes 93 formed in No. 1 with the stimulable phosphor.

In the present embodiment, the surface of the dot-shaped stimulable phosphor layer region 92 is located above the surface of the support 91, and the stimulable phosphor is provided in the large number of through holes 93. It is filled.

Here, the large number of through holes 93 are supported in the same regular pattern as the large number of through holes 3 and the dot-shaped absorptive regions 4 formed in the substrate 2 of the biochemical analysis unit 1. Each stimulable phosphor layer region 92 is formed on the substrate 2 of the biochemical analysis unit 1 when the stimulable phosphor sheet 90 is superposed on the biochemical analysis unit 1 when formed on the body 91. The stimulable phosphor sheet 90 is configured so as to face only the corresponding absorptive region 4 in the corresponding through hole 3.

FIG. 18 shows a large number of dot-shaped absorptive regions 4
FIG. 6 is a schematic cross-sectional view showing a method of exposing a large number of dot-shaped stimulable phosphor layer regions 92 formed on a stimulable phosphor sheet 90 with a radioactive labeling substance contained in.

As shown in FIG. 18, during exposure, a large number of through holes 3 formed in the support 91 of the stimulable phosphor sheet 90 are filled with stimulable phosphors. The dot-shaped stimulable phosphor layer regions 92 are located in a large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1, respectively, and face the absorptive region 4 in the through holes 3. Then, the stimulable phosphor sheet 90 is overlaid on the biochemical analysis unit 1.

Also in the present embodiment, upon exposure, an electron beam is emitted from the radioactive labeling substance contained in the large number of dot-shaped absorptive regions 4, but the substrate 2 of the biochemical analysis unit 1 attenuates the radiation. Since it is formed of a metal, the electron beam emitted from the radiolabel substance is reliably prevented from being scattered in the substrate 2, and a large number of dot-shaped stimulable phosphor layer regions 92 are biochemical. With a large number of through holes 3 and a large number of dot-shaped absorptive regions 4 formed in the substrate 2 of the analysis unit 1, in the same regular pattern,
The stimulable phosphor sheet 90 is formed on the biochemical analysis unit 1 so as to face the openings of the large number of through holes 3 formed on the substrate 81 of the biochemical analysis unit 1, respectively. Since they are superposed, the electron beam emitted from the radioactive labeling substance contained in the absorptive region 4 in each through hole 3 formed in the substrate 2 has a corresponding dot-shaped stimulable phosphor layer 92. Incident only on the support 91
Is formed of silicon nitride, which has a property of attenuating radiation, so that the electron beam can be stored in the phosphor sheet 90.
Scattering within the support 91 is also prevented, and therefore the corresponding dot-shaped stimulable fluorescence is generated by the radioactive labeling substance contained in the adsorptive region 4 in each through hole 3 formed in the substrate 2. It becomes possible to reliably expose only the body layer 92, and therefore, the stimulable phosphor layer area 92 to be exposed by the radiolabel substance contained in the absorptive area 4 in each through hole 3 is adjacent to the stimulable phosphor layer area 92. It is possible to reliably prevent generation of noise in the biochemical analysis data, which is caused by exposure to the electron beam emitted from the radio-labeled substance contained in the adsorptive region 4 in the through hole 3. It is possible to significantly improve the quantitativeness of biochemical analysis.

FIG. 19 is a schematic partial sectional view of a stimulable phosphor sheet according to still another preferred embodiment of the present invention.

As shown in FIG. 19, the stimulable phosphor sheet 100 according to this embodiment is formed on the surface of the support 101 in a regular pattern with the support 101 formed of polyethylene terephthalate. In addition, a large number of dot-shaped stimulable phosphor layer regions 102 are provided.

Here, a large number of dot-shaped stimulable phosphor layer regions 102, a large number of through holes 3 and dot-shaped absorptive regions 4 formed in the substrate 2 of the biochemical analysis unit 1,
When the stimulable phosphor sheet 100 is superposed on the biochemical analysis unit 1, it is formed on the surface of the support 101 in the same regular pattern and with the same size and in a substantially circular shape. , Each stimulable phosphor layer region 102
However, the stimulable phosphor sheet 100 is configured so as to face only the absorptive region 4 in the corresponding through hole 3 formed on the substrate 2 of the biochemical analysis unit 1.

FIG. 20 shows a large number of dot-shaped stimulable phosphor layer regions 102 formed on the stimulable phosphor sheet 100 by the radioactive labeling substance contained in the absorptive regions 4 in the large number of through holes 3. It is a schematic sectional drawing which shows the method of exposing.

As shown in FIG. 20, upon exposure,
A large number of dot-shaped stimulable phosphor layer regions 102 formed on the surface of the support 101 of the stimulable phosphor sheet 100 are
The stimulable phosphor sheet 100 is superposed on the biochemical analysis unit 1 so as to be located in each of the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1.

Also in this embodiment, during exposure, an electron beam is emitted from the radiolabeled substance contained in the large number of dot-shaped absorptive regions 4, but the substrate 2 of the biochemical analysis unit 1 attenuates the radiation. Since it is formed of a metal having a property, the electron beam emitted from the radioactive labeling substance is surely prevented from being scattered in the substrate 2, and a large number of dot-shaped stimulable phosphor layer regions 102 are formed. , A large number of through holes 3 and a large number of dot-shaped absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are formed on the surface of the support 101 in the same regular pattern, respectively. Since the stimulable phosphor sheet 100 is superposed on the biochemical analysis unit 1 so as to be located in the large number of through holes 3 formed on the substrate 2 of the biochemical analysis unit 1, the stimulable phosphor sheet 100 is formed on the substrate 2. The electron beam emitted from the radioactive labeling substance contained in the adsorptive region 4 in each of the through holes 3 is incident only on the corresponding dot-shaped stimulable phosphor layer 102, and further, the support 101 Is formed of polyethylene terephthalate, which has a property of attenuating radiation, so that the electron beam is also prevented from being scattered within the support 101 of the stimulable phosphor sheet 100, and therefore each through hole formed in the substrate 2 is prevented. The radioactive labeling substance contained in the absorptive region 4 in 3 makes it possible to reliably expose only the corresponding dot-shaped stimulable phosphor layer 102, and therefore the adsorption in each through hole 3 Of the stimulable phosphor layer 102 to be exposed by the radioactive labeling substance contained in the absorptive region 4 is the absorptive region 4 in the adjacent through hole 3.
It is possible to reliably prevent the generation of noise in the data for biochemical analysis, which is caused by exposure to the electron beam emitted from the radiolabeled substance contained in, and the quantification of biochemical analysis is possible. Can be significantly improved.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the embodiment shown in FIGS. 1 to 13, the embodiment shown in FIG. 14 and the embodiment shown in FIG. 15, the control unit 70 controls the intermittent movement of the optical head 35. Synchronously, first
The laser excitation light source 21 is controlled to be turned on and off,
In the main scanning direction, if the moving speed of the optical head 35 in the main scanning direction is determined so that the laser light 24 moves quickly between the adjacent dot-shaped photostimulable phosphor layer regions 12, the first Holding the laser excitation light source 21 of
The optical head 35 is simply moved intermittently, and a large number of dot-shaped photostimulable phosphor layer regions 12 are sequentially scanned by the laser light 24 to form dot-shaped photostimulable phosphor layer regions 12. It is also possible to photoelectrically detect the photostimulable light 45 emitted from the device to generate biochemical analysis data.

Further, in the embodiment shown in FIG. 14, when the optical head 35 is intermittently moved in the main scanning direction and the first laser excitation light source 21 is on / off controlled, an integrating amplifier is used. 75 is used to integrate the analog signal output from the photomultiplier 50.
Also in the embodiment shown in FIG. 16 configured to continuously move the optical head 35 in the main scanning direction at a constant speed, an analog amplifier output from the photomultiplier 50 is used by using an integrating amplifier. It can be configured to integrate the signal.

Further, in the embodiment shown in FIG. 15, when the optical head 35 is intermittently moved in the main scanning direction and the first laser excitation light source 21 is on / off controlled, the adding means is added. The digital signal output from the A / D converter 53 is added by using 77, but the optical head 35 is configured to be continuously moved in the main scanning direction at a constant speed. In the embodiment shown in FIG. 8 also, the adding means 77 can be used to add the digital signals output from the A / D converter 53.

Further, in the above-mentioned embodiment, a plurality of cDNAs having different known base sequences are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to the cDNA. Not hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
All specific binding substances, such as A and RNA, that can be specifically bound to substances of biological origin and whose base sequence, base length, composition, etc. are known, should be used as the specific binding substance of the present invention. You can

Further, in the above-mentioned embodiment, the substance of biological origin labeled with the radioactive labeling substance or the fluorescent substance is hybridized with the specific binding substance, but the substance of biological origin is changed to the specific binding substance. It is not always necessary to hybridize, and instead of using a substance derived from a living body for hybridization, an antigen-antibody reaction,
It is also possible to specifically bind to a specific binding substance by a reaction such as a receptor / ligand.

Further, in the above-mentioned embodiment, the large number of dot-shaped absorptive regions 4 of the biochemical analysis unit 1 are
Nylon 6 capable of forming a membrane filter is filled inside a large number of through holes 3 formed in the substrate 2, but formed in the substrate 2 instead of the large number of through holes 3. However, a porous material may be embedded inside the large number of recesses to form a large number of dot-shaped absorptive regions 4.

Further, in the above-mentioned embodiment, a large number of dot-shaped absorptive regions 4 of the biochemical analysis unit 1 can form a membrane filter inside the large number of through holes 3 formed in the substrate 2. Nylon 6 is filled and formed, but the absorptive region 4 of the biochemical analysis unit 1 is
It is not always necessary to be formed of nylon 6, and instead of nylon 6, a carbon porous material such as activated carbon or nylons such as nylon 6,6 and nylon 4,10; nitrocellulose, cellulose acetate, butyric acid acetic acid Cellulose derivatives such as cellulose; collagen;
Alginic acids such as alginic acid, calcium alginate, alginic acid / polylysine polyion complex, etc .;
Polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polytetrafluoride, and other polyfluorides, and their adsorbability of the biochemical analysis unit 1 by a copolymer or complex thereof. It is also possible to form the region 4 and the adsorptive region 84 of the biochemical analysis unit 80, and further, a metal such as platinum, gold, iron, silver, nickel or aluminum; a metal oxidation such as alumina, silica, titania or zeolite. The substance; the absorptive region 4 of the biochemical analysis unit 1 may be formed of an inorganic porous material such as a metal salt of hydroxyapatite or calcium sulfate, a complex thereof, or a bundle of a plurality of fibers.

Further, in the above-mentioned embodiment, the biochemical analysis unit 1 is constructed such that nylon 6 is filled inside the large number of through-holes 3 formed in the substrate 2, and the absorptive region 4 thus formed is provided with the cDNA. It is formed by dropping a specific binding substance such as, for example, and hybridizing a substance of biological origin labeled with a radioactive labeling substance to the specific binding substance, but it is formed by an adsorptive material such as a porous material. A specific binding substance was dropped in spots on an absorptive substrate such as a porous substrate, and a bio-derived substance labeled with a radioactive labeling substance was hybridized to obtain the obtained biochemical analysis unit 1. By using the stimulable phosphor sheet 10, 9
0,100 dot-shaped stimulable phosphor layer regions 12, 9
2, 102 may be exposed.

Further, in the embodiment shown in FIGS. 1 to 13 and the embodiment shown in FIG. 15, the support 11 of the stimulable phosphor sheet 10 is made of stainless steel, and FIG. And in the embodiment shown in FIG. 17, the support 91 of the stimulable phosphor sheet 90 is
A stimulable phosphor sheet 100 formed of silicon nitride, and in the embodiment shown in FIGS.
The support 101 is made of polyethylene terephthalate, and the stimulable phosphor sheets 10, 90,
It is not always necessary to form the supports 11, 91, 101 of 100 by stainless steel, silicon nitride, or polyethylene terephthalate, and it is also possible to form by other materials. In the present invention, the support 11 of the stimulable phosphor sheet 10 is preferably made of a material having a property of attenuating radiation. Instead of stainless steel, silicon nitride or polyethylene terephthalate, for example, gold, silver, Copper, aluminum, nickel,
Zinc, titanium, tantalum, chromium, iron, cobalt, lead,
Metal materials such as tin and brass or their alloys such as stainless steel, aluminum oxide, magnesium oxide, zirconium oxide, silicon carbide, ceramic materials such as tungsten carbide, polyolefins such as polyethylene and polypropylene, polystyrene, polymethylmethacrylate. Acrylic resin such as, polyvinyl chloride, polyvinylidene chloride, polyvinylidene fluoride, polytetrafluoroethylene, polychlorotrifluoroethylene, polycarbonate, polyester such as polyethylene naphthalate, nylon such as nylon 6, nylon 66, polyimide, polysulfone, Silicon resin such as polyphenylene sulfide and polydiphenyl siloxane, phenol resin such as novolac, epoxy resin,
The support 11, 9 of the stimulable phosphor sheet 10, 90, 100 is made of polyurethane, cellulose such as cellulose acetate or nitrocellulose, or plastic material such as copolymer such as butadiene-styrene copolymer.
1, 101 can be formed.

Further, in the above embodiment, the dot-shaped stimulable phosphor layer regions 12, 92, 102 are all provided with a large number of through holes 3 and holes formed in the substrate 2 of the biochemical analysis unit 1. Although the dot-shaped stimulable phosphor layer regions 12, 92 and 102 are formed in a substantially circular shape having the same size as the dot-shaped absorptive region 4, it is not always necessary to form the dot-shaped stimulable phosphor layer regions 12, 92, 102 in a substantially circular shape. Alternatively, the dot-shaped stimulable phosphor layer regions 12, 92, 102 may be formed in a shape other than a circle, such as a substantially rectangular shape.
It is not always necessary to form the biochemical analysis unit 1 so as to have the same size as the through holes 3 and the dot-shaped absorptive regions 4.

Further, in the above-mentioned embodiment, the dot-shaped stimulable phosphor layer regions 12, 92, 102 are provided with a large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1.
And the dot-shaped stimulable phosphor layer regions 12, 92, 102 are formed on the substrate 2 of the biochemical analysis unit 1 in the same regular pattern as the dot-shaped absorptive region 4. It is sufficient that the through holes 3 and the dot-shaped absorptive regions 4 are formed in the same pattern as the formed through holes 3,
It is also possible to form a large number of through holes 3 and dot-shaped absorptive regions 4 on the substrate 2 of the biochemical analysis unit 1 in a regular pattern, or to form the dot-shaped stimulable phosphor layer regions 12, 9
It is not always necessary to form 2, 102 in a regular pattern.

Further, in the above-mentioned embodiment, the dot-shaped stimulable phosphor layer regions 12, 92, 102 have a large number of through holes 3 and a large number of dots formed in the substrate 2 of the biochemical analysis unit 1. Dot-shaped stimulable phosphor layer regions 12, 92, 102 formed in the same regular pattern as the absorptive region 4 and thus having a diameter of about 0.01 square millimeter of about 10,000. About 5000 /
With a density of square centimeters, the support 11, regularly,
The number and size of the large number of through holes 3 and the dot-shaped absorptive regions 4 formed in 91 and 101 can be arbitrarily selected according to the purpose, and preferably 1
0 or more through-holes 3 and dot-shaped absorptive regions 4 are 1
The density of 0 pieces / square centimeter or more is formed on the substrate 2 of the biochemical analysis unit 1, and the through holes 3 and the absorptive regions 4 are formed to a size of less than 5 square millimeters. Therefore, the stimulable phosphor sheet 10 is formed. , 90, 10
The number and size of the dot-shaped stimulable phosphor layer regions 12, 92, 102 formed on the supports 11, 91, 101 of No. 0 can be arbitrarily selected according to the purpose, but are accumulated. Support 1 for the fluorescent phosphor sheet 10, 90, 100
1, 50, 50 or more dot-shaped stimulable phosphor layer regions 12, 92, 102 are formed in 1, 91, 101 at a density of 50 / cm 2 or more, and their size is less than 5 mm 2. Is preferred.

Furthermore, in the above-mentioned embodiment, a large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1 are used.
Also, the large number of dot-shaped absorptive regions 4 and the dot-shaped stimulable phosphor layer regions 12, 92, 102 are formed two-dimensionally, but they may be formed one-dimensionally.

Furthermore, in the embodiment shown in FIGS. 1 to 13 and the embodiment shown in FIG. 15, a large number of dot-shaped stimulable phosphor layer regions 12 have their surfaces
The support 11 is positioned above the surface of the support 11.
In the embodiment shown in FIGS. 16 and 17, a large number of dot-shaped stimulable phosphors are formed by embedding the stimulable phosphor in the large number of recesses 13 formed in FIG. The layer region 92 is formed by filling a large number of through holes 93 formed in the support 91 with a stimulable phosphor so that the surface thereof is located above the surface of the support 91. In the embodiments shown in FIGS. 18 and 19, a large number of dot-shaped photostimulable phosphor layer regions 102 are formed on the surface of the support 101, and all of them are formed into a large number of dot-shaped bright phosphors. A large number of dot-shaped stimulable phosphor layer regions 12, so that the surfaces of the stimulable phosphor layer regions 12, 92, 101 are located above the surfaces of the supports 11, 91, 101.
Although 92 and 101 are formed, a large number of dot-shaped stimulable phosphor layer regions 12, 92 and 101 are arranged so that their surfaces are located above the surfaces of the supports 11, 91 and 101. It is not always necessary to form it, and even if a large number of dot-shaped stimulable phosphor layer regions 12, 92, 101 are formed so that their surfaces are at the same height as the surfaces of the supports 11, 91, 101. It may be formed so as to be located below the surface of the supports 11, 91, 101.

Further, in the above-mentioned embodiment, the scanner has a large number of dot-shaped stimulable phosphor layer regions 12, 92 formed on the stimulable phosphor sheets 10, 90, 100.
Radioactivity data of the radiolabeled substance recorded in 102 and biochemical analysis unit 1 contained in a dot-shaped absorptive region 4 in a large number of through holes 3 formed in the substrate 2 c
It is configured to read fluorescence data of a fluorescent substance such as a fluorescent dye that labels a substance of biological origin hybridized with a specific binding substance such as DNA, and generate digital data for biochemical analysis, A first laser excitation light source 21 that emits laser light 24 having a wavelength of 640 nm;
The scanner is provided with the second laser excitation light source 22 which emits the laser light 24 having the wavelength of 32 nm and the third laser excitation light source 23 which emits the laser light 24 having the wavelength of 473 nm. , 90, 100 formed into a large number of dot-shaped stimulable phosphor layer regions 12, 92,
It suffices that the radiation data of the radiolabeled substance recorded in 102 be read to generate digital data for biochemical analysis, and a large number of through holes formed in the substrate 2 of the biochemical analysis unit 1 3 reads the fluorescence data of a fluorescent substance such as a fluorescent dye that labels a substance of biological origin that is hybridized with a specific binding substance such as cDNA contained in the dot-shaped adsorptive region 4 It is not always necessary to be able to generate digital data for chemical analysis, and therefore 640 nm
First laser excitation light source 2 for emitting laser light 24 of
The laser light 2 having a wavelength of 532 nm should be provided
Second laser excitation light source 22 which emits 4 and third laser excitation light source 23 which emits laser light 24 having a wavelength of 473 nm.
It is not always necessary to have and.

Further, in the above-described embodiment, the optical head 35 in the scanner has the X direction and the Y direction in FIG.
Although it is configured to be movable in the direction,
It is sufficient if the stimulable phosphor sheets 10, 90, 100 are configured to be movable in the X direction and the Y direction, and the stage 40 is movable in the X direction and the Y direction in FIG. The optical head 35 may be configured to be movable in the X direction or the Y direction, and the stage 40 may be configured to be movable in the Y direction or the X direction. Stage 40 is X
When configured to move in the direction
By providing a linear encoder in the moving mechanism, the relative position of the stage 40 with respect to the optical head 35 is detected, or the rotational position of the motor for moving the stage 40 is detected by the rotary encoder to detect the optical head 35. The relative position of the stage 40 with respect to can be detected.

Further, in the above embodiment, the position of the optical head 35 in the main scanning direction is detected by using the linear encoder 67, but the rotational position of the main scanning stepping motor 65 or the main scanning motor 80 is detected. Thus, the position of the optical head 35 in the main scanning direction can be detected.

Further, in the above embodiment, the scanner is equipped with the semiconductor laser light source which emits the laser light 24 having the wavelength of 640 nm as the first laser excitation light source 21, but emits the laser light 24 having the wavelength of 640 nm. Instead of the semiconductor laser light source, laser light with a wavelength of 635 nm 2
4 may be provided, or a He—Ne laser light source that emits laser light 24 having a wavelength of 633 nm may be provided.

Further, in the above embodiment, the scanner includes the first laser excitation light source 21 which emits the laser light 24 having the wavelength of 640 nm and the laser light 24 having the wavelength of 532 nm.
The second laser excitation light source 22 which emits the laser light and the third laser excitation light source 23 which emits the laser light 24 having a wavelength of 473 nm are provided, but it is not always necessary to use the laser excitation light source as the excitation light source, Instead of the laser excitation light source, an LED light source can be used as the excitation light source,
Further, a halogen lamp may be used as an excitation light source, and a spectral filter may be used to cut off wavelength components that do not contribute to excitation of the stimulable phosphor.

In the above embodiment, the photomultiplier 50 is used as the photodetector, and the photostimulable photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer regions 12, 92, 102 is photoelectrically converted. However, the photodetector used in the present invention is not limited to the photomultiplier 50 and may be another photodetector such as a CCD as long as it can photoelectrically detect the stimulated emission 45. Can be used.

[0275]

INDUSTRIAL APPLICABILITY According to the present invention, a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. is labeled with a radiolabeling substance. The radiolabeled substance contained in the spot-shaped region is included even when the spot-shaped region selectively labeled with a substance derived from a living body is formed at high density on the surface of a carrier such as a membrane filter. The exposed photostimulable phosphor layer is scanned with excitation light, and photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected,
It is possible to provide a biochemical analysis data generation method capable of generating biochemical analysis data excellent in quantification and a scanner used therefor.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic cross-sectional view of a hybridization container.

FIG. 4 is a schematic perspective view of a stimulable phosphor sheet according to a preferred embodiment of the present invention.

FIG. 5 is a method for exposing a large number of dot-shaped stimulable phosphor layer regions formed on a stimulable phosphor sheet by using a radioactive labeling substance contained in a large number of dot-shaped porous regions. FIG.

FIG. 6 is a schematic perspective view showing an example of a scanner.

FIG. 7 is a schematic perspective view showing details of the vicinity of the photomultiplier.

8 is a schematic cross-sectional view taken along the line AA of FIG.

9 is a schematic cross-sectional view taken along the line BB of FIG.

10 is a schematic cross-sectional view taken along the line CC of FIG.

11 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 12 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 13 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner according to the preferred embodiment of the present invention.

FIG. 14 is a block diagram of the periphery of a photomultiplier and data processing device of a scanner according to another preferred embodiment of the present invention.

FIG. 15 is a block diagram around a photomultiplier and data processing device of a scanner according to another preferred embodiment of the present invention.

FIG. 16 is a block diagram showing a control system, an input system, a drive system and a detection system of a scanner according to another preferred embodiment of the present invention.

FIG. 17 is a schematic partial cross-sectional view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.

FIG. 18 shows a method of exposing a large number of dot-shaped stimulable phosphor layer regions formed on a stimulable phosphor sheet with a radioactive labeling substance contained in a large number of dot-shaped porous regions. It is a schematic sectional drawing shown.

FIG. 19 is a schematic partial cross-sectional view of a stimulable phosphor sheet according to still another preferred embodiment of the present invention.

FIG. 20 shows a method of exposing a large number of dot-shaped stimulable phosphor layer regions formed on a stimulable phosphor sheet with a radioactive labeling substance contained in a large number of dot-shaped porous regions. It is a schematic sectional drawing shown.

[Explanation of symbols]

1 Biochemical analysis unit 2 substrates 3 through holes 4 Dot-shaped porous area 5 Spotting device 6 injectors 7 CCD camera 8 Hybridization container 9 Hybridization liquid 10 Storage phosphor sheet 11 Support 12-dot stimulable phosphor layer area 13 recess 21 First laser excitation light source 22 Second laser excitation light source 23 Third Laser Excitation Light Source 24 laser light 25 Collimator lens 26 mirror 27 First Dichroic Mirror 28 Second dichroic mirror 29 mirror 30 collimator lens 31 Collimator lens 32 mirror 33 holes for the perforated mirror 34 perforated mirror 35 Optical head 36 mirror 37 Aspherical lens 38 concave mirror 40 stages 41 glass plate 45 Bright or fluorescent 48 filter units 50 Photomultiplier 51a, 51b, 51c, 51d filter member 52a, 52b, 52c, 52d filters 53 A / D converter 54 Data processing device 60 substrates 61 Sub-scanning pulse motor 62 a pair of rails 63 Movable substrate 64 rod 65 Main scanning stepping motor 66 endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 keyboard 72 Filter unit motor 75 integrating amplifier 76 data memory 77 Addition means 78 data memory 80 main scanning motor 90 Storage phosphor sheet 91 Support 92 Dot-shaped stimulable phosphor layer region 93 through hole 100 Phosphor sheet 101 support 102 Dot-shaped stimulable phosphor layer region

Front page continuation (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 37/00 102 G01T 1/00 B 2H013 G01T 1/00 1/29 D 4B063 1/29 G03B 42/02 B G03B 42 / 02 G01N 35/06 A (72) Inventor Nobuhiko Ogura No.798 Miyadai, Kaisei-cho, Ashigaragami-gun, Kanagawa Fuji Photo F Co., Ltd. F-term (reference) 2G045 AA35 DA12 DA13 DA14 FA12 FB02 FB07 FB08 FB12 GC15 JA01 2G054 AB07 CA22 CE02 EA03 FA50 FB01 GA04 GB02 GE05 2G058 AA09 CC02 EA11 ED11 2G083 AA03 BB04 CC02 CC04 DD01 DD12 DD20 EE02 2G088 EE27 JJ05 JJ30 2H013 AC01 AC04 AC06 4B063 QA01 QA13 QA18 QQ42 Q02 QR52

Claims (41)

[Claims] 1. A plurality of stimulable phosphor sheets each comprising a support, and a plurality of dot-shaped photostimulable phosphor layer regions formed on the support at least one-dimensionally separated from each other. In the dot-shaped stimulable phosphor layer region, after selectively storing radiation energy, the stimulable phosphor sheet and excitation light are relatively moved at least in the main scanning direction, In the dot-shaped stimulable phosphor layer area of,
Sequentially, by irradiating the excitation light, to excite the stimulable phosphor contained in the plurality of dot-shaped stimulable phosphor layer region, the stimulable light emitted from the stimulable phosphor Is detected photoelectrically to generate an analog signal, and the analog signal is converted into a digital signal to generate biochemical analysis data, and a biochemical analysis data generation method. 2. The energy per unit area of the excitation light with which the plurality of dot-shaped stimulable phosphor layer regions is irradiated is higher than that of the regions other than the plurality of dot-shaped stimulable phosphor layer regions. The method for generating biochemical analysis data according to claim 1, further comprising irradiating the excitation light so as to increase the temperature. 3. The plurality of dot-shaped stimulable phosphor layer regions are two-dimensionally formed on the support so as to be separated from each other, and the stimulable phosphor sheet and the excitation light are mainly formed. In the sub-scanning direction orthogonal to the scanning direction and the main scanning direction, relatively moved, to the plurality of dot-shaped stimulable phosphor layer regions, sequentially irradiating the excitation light, the plurality of dots Exciting stimulable phosphor contained in the stimulable phosphor layer region of the shape, photoelectrically detecting the stimulable light emitted from the stimulable phosphor, to generate an analog signal, The method for generating biochemical analysis data according to claim 1, wherein the analog signal is converted into a digital signal to generate biochemical analysis data. 4. The stimulable phosphor sheet and the excitation light are relatively and intermittently moved in the main scanning direction to form a plurality of dot-shaped stimulable phosphor layer regions, The method for generating biochemical analysis data according to any one of claims 1 to 3, wherein the excitation light is irradiated for a predetermined time. 5. The excitation light is constantly applied to the storage phosphor sheet while the storage phosphor sheet and the excitation light are relatively and intermittently moved in the main scanning direction. The method for generating biochemical analysis data according to claim 4, wherein irradiation is performed. 6. The excitation light is irradiated only to the plurality of dot-shaped photostimulable phosphor layer regions, and the excitation light is irradiated to regions other than the plurality of dot-shaped photostimulable phosphor layer regions. The method for generating biochemical analysis data according to claim 4, wherein the excitation light is controlled to be turned on / off so as not to be irradiated. 7. The stimulable phosphor sheet and the excitation light are relative to each other in the main scanning direction at a pitch equal to the distance between the dot-shaped stimulable phosphor layer regions adjacent to each other in the main scanning direction. The method for generating biochemical analysis data according to any one of claims 4 to 6, characterized in that it is intermittently moved. 8. The stimulable phosphor sheet and the excitation light are relatively and continuously moved in the main scanning direction,
Substantially, only the plurality of dot-shaped stimulable phosphor layer regions are irradiated with the excitation light, the region other than the plurality of dot-shaped stimulable phosphor layer regions, the excitation light is irradiated. The method for generating biochemical analysis data according to any one of claims 1 to 3, wherein the excitation light is controlled to be turned on / off so as not to be performed. 9. The raw material according to claim 1, wherein the analog signal is integrated, and the integrated value of the analog signal is digitized to generate biochemical analysis data. Method for generating data for chemical analysis. 10. The biochemical analysis data generating method according to claim 1, wherein the digital signals are added to generate biochemical analysis data. 11. The biochemical analysis data generation method according to claim 1, wherein the stimulable phosphor sheet is moved in the main scanning direction. 12. The method for generating biochemical analysis data according to claim 1, wherein the excitation light is moved in the main scanning direction. 13. A plurality of holes are formed in the support of the stimulable phosphor sheet so as to be spaced apart from each other, and the plurality of dot-shaped stimulable phosphor layer regions are formed in the plurality of holes. 13. The biochemical analysis data generation method according to claim 1, wherein the biodegradable phosphor is embedded and formed. 14. The dot-shaped stimulable phosphor layer region is formed on the support of the stimulable phosphor sheet, as claimed in any one of claims 1 to 12. The method for generating biochemical analysis data described in. 15. The stimulable phosphor layer region having a plurality of dots is formed in a regular pattern on the support of the stimulable phosphor sheet.
15. The method for generating biochemical analysis data according to any one of 1 to 14. 16. The stimulable phosphor layer region in the form of 10 or more dots is formed on the support of the stimulable phosphor sheet, as described in any one of claims 1 to 15. The method for generating biochemical analysis data described in. 17. The stimulable phosphor layer region in the form of dots is formed in a size of less than 5 square millimeters, respectively. Method for generating data for biochemical analysis. 18. The plurality of dot-shaped stimulable phosphor layer regions are provided on the support of the stimulable phosphor sheet in an amount of 10 times.
18. The method for generating biochemical analysis data according to claim 1, wherein the biochemical analysis data is formed at a density of not less than one piece / square centimeter. 19. The biochemical analysis data according to claim 1, wherein the support of the stimulable phosphor sheet is formed of a material that attenuates radiation. Generation method. 20. When radiation is transmitted through the support by a distance equal to the distance between adjacent dot-shaped stimulable phosphor layer regions of the support of the stimulable phosphor sheet. The biochemical analysis data generation method according to claim 19, wherein the biochemical analysis data is formed of a material having a property of reducing the energy of radiation to 1/5 or less. 21. The raw material according to claim 19, wherein the support of the stimulable phosphor sheet is made of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. Method for generating data for chemical analysis. 22. Laser light is used as excitation light, and the stimulable phosphor sheet and the laser light are relatively moved in a main scanning direction and a sub-scanning direction orthogonal to the main scanning direction, and the plurality of the plurality of sheets are stored. The dot-shaped stimulable phosphor layer region is sequentially irradiated with the laser light to excite the stimulable phosphor contained in the plurality of dot-shaped stimulable phosphor layer regions. 22. The method for generating biochemical analysis data according to any one of claims 2 to 21. 23. An excitation light source that emits excitation light, and a stimulable phosphor sheet having a plurality of dot-shaped stimulable phosphor layer regions that are formed separately from each other and selectively accumulate radiation energy are mounted. A sample stage that can be placed, an irradiation optical system that directs the excitation light emitted from the excitation light source to the sample stage, and the excitation light emitted from the excitation light source causes the plurality of dot-shaped photostimulability. A scanning mechanism that relatively moves the irradiation optical system and the sample stage in at least the main scanning direction so that the phosphor layer region is sequentially irradiated, and the irradiation light emitted from the excitation light source. With the excitation light directed to the sample stage by the system, the plurality of dot-shaped stimulable phosphor layer regions are excited, and the emitted stimulable light is photoelectrically detected. And a photodetector for generating an analog signal, and an A / D converter for digitizing the analog signal generated by the photodetector to generate a digital signal. 24. Excitation control means for controlling the excitation light source and the scanning mechanism, and position detection means for detecting a relative positional relationship between the irradiation optical system and the sample stage in the main scanning direction, The excitation control means, based on the relative positional relationship in the main scanning direction between the irradiation optical system and the sample stage detected by the position detection means, the plurality of dot-shaped stimulable phosphor layer regions The energy per unit area of the excitation light irradiated to the region is controlled to be higher than the regions other than the plurality of dot-shaped stimulable phosphor layer regions, and the excitation light source and the scanning mechanism are controlled. 24. The scanner according to claim 23, which is configured as described above. 25. The scanning mechanism is configured to relatively move the irradiation optical system and the sample stage in the main scanning direction and a sub scanning direction orthogonal to the main scanning direction. Claim 23 or 2 characterized by the above-mentioned.
The scanner according to item 4. 26. The excitation control means controls the scanning mechanism so as to relatively and intermittently move the irradiation optical system and the sample stage in the main scanning direction, and 24. The excitation light source is controlled so that the plurality of dot-shaped stimulable phosphor layer regions are respectively irradiated with the excitation light for a predetermined time. The scanner according to any one of items 1 to 25. 27. While the excitation control means moves the irradiation optical system and the sample stage relatively and intermittently in the main scanning direction, the excitation light is always stored in the storage property. 27. The scanner of claim 26, configured to control the excitation light source to illuminate a phosphor sheet. 28. The excitation control means irradiates the excitation light only to the plurality of dot-shaped stimulable phosphor layer regions, and forms a region other than the plurality of dot-shaped stimulable phosphor layer regions. 27. The scanner according to claim 26, which is configured to control ON / OFF of the excitation light source so that the excitation light is not irradiated. 29. The scanning mechanism comprises the irradiation optical system and the sample stage at a pitch equal to a distance between the dot-shaped stimulable phosphor layer regions adjacent to each other in the main scanning direction. 29. The scanner according to claim 26, wherein the scanner is configured to be moved relatively and intermittently. 30. The scanning mechanism is configured to move the irradiation optical system and the sample stage relatively and continuously in the main scanning direction, and the excitation control means is substantially In, only the plurality of dot-shaped stimulable phosphor layer regions, the excitation light is irradiated, the region other than the plurality of dot-shaped stimulable phosphor layer regions, the excitation light is not irradiated 26. The scanner according to claim 23, wherein the scanner is configured to control the excitation light source to be turned on and off. 31. The scanner according to claim 23, further comprising an integrating unit that integrates an analog signal generated by the photodetector. 32. The A / D converter further comprises:
31. The scanner according to claim 23, further comprising adding means for adding the generated digital signals. 33. The scanner according to claim 23, wherein the scanning mechanism is configured to move the sample stage in a main scanning direction. 34. The scanner according to claim 33, wherein the position detecting means is composed of a linear encoder that detects the position of the sample stage in the main scanning direction. 35. The scanning mechanism comprises a stepping motor for intermittently moving the sample stage in the main scanning direction.
Scanner as described in. 36. The scanner according to claim 35, wherein the position detecting means is constituted by a rotary encoder that detects a rotational position of the stepping motor. 37. The scanning mechanism controls the irradiation optical system,
33. The scanner according to claim 23, wherein the scanner is configured to move in the main scanning direction. 38. The scanner according to claim 37, wherein the position detecting means is composed of a linear encoder that detects a position of the irradiation optical system in the main scanning direction. 39. The scanning mechanism controls the irradiation optical system,
39. The scanner according to claim 37, further comprising a stepping motor that intermittently moves in the main scanning direction. 40. The scanner according to claim 39, wherein the position detecting means is a rotary encoder that detects a rotational position of the stepping motor. 41. The scanner according to claim 23, wherein the excitation light source is a laser excitation light source that emits laser light.