JP2003295365A - Scanner - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a scanner which can generate data with high sensitivity by converging light emitted non-directionally as a stimulable phosphor, a fluorescent substance, etc., included in a sample are excited with exciting light. <P>SOLUTION: The scanner is equipped with exciting light sources 21, 22, and 23 which emit exciting lights, a sample stage 40 where the sample is mounted, a convergence optical system 37 which converges light emitted as the sample is scanned with the exciting light 24, a photodetector 50 which photoelectrically detects the converged light, a scanning means which relatively moves the optical path of the exciting light and the convergence optical system in horizontal and vertical scanning directions, and reflection optical systems 39a and 39c which are provided nearby the sample and reflect the light emitted from the sample to the sample, the reflection optical system being moved relatively to the sample stage. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a scanner, and more specifically, it has a simple structure and excites a stimulable phosphor or a fluorescent substance contained in a sample by excitation light. The present invention relates to a scanner that can collect light emitted omnidirectionally efficiently and generate data with high sensitivity.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave in a specific wavelength range. The photostimulable phosphor layer provided on the stimulable phosphor sheet is used for the energy of the radiation transmitted through the object by using the photostimulable phosphor having a characteristic of emitting a stimulating amount of light as a radiation detection material. In the stimulable phosphor contained in, was accumulated and recorded, after which, by electromagnetic waves, the stimulable phosphor layer was scanned to excite the stimulable phosphor, which was emitted from the stimulable phosphor. Radiation that is configured to photoelectrically detect stimulated light, generate a digital image signal, perform image processing, and reproduce a radiation image on a display material such as a CRT or a recording material such as a photographic film. Image diagnostic system knows To have (for example, JP-A-55-
No. 12429, No. 55-116340, No. 5
No. 5-163472, No. 56-11395,
No. 56-104645, etc. ).

Further, a similar stimulable phosphor is used as a radiation detecting material, a substance having a radioactive label is administered to an organism, and then the organism or a part of the tissue of the organism is sampled. And, this sample, by accumulating a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time, the radiation energy is stored in the stimulable phosphor, recorded, and then, by electromagnetic waves, Scan the photostimulable phosphor layer to excite the photostimulable phosphor, photoelectrically detect the photostimulable light emitted from the photostimulable phosphor, generate a digital image signal, and perform image processing. There is known an autoradiography analysis system configured to reproduce an image on a display means such as a CRT or a recording material such as a photographic film (for example, Japanese Patent Publication No. 1-70884).
Japanese Patent Publication, Japanese Patent Publication No. 1-70882, Japanese Patent Publication No. 4-39
62 publication).

The autoradiography analysis system using the stimulable phosphor sheet as a radiation detecting material is not only required to be chemically treated as a developing treatment, unlike the case where a photographic film is used, but also obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

[0006] Similarly, a specific binding substance such as protein or nucleic acid is immobilized on a biochemical analysis unit such as a membrane filter and is labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A substance derived from a living body is specifically bound to a specific binding substance,
Visible light generated by contact between the chemiluminescent substrate and the labeling substance by bringing the chemiluminescent substrate into contact with the binding substance of the specific binding substance selectively labeled with the labeling substance and the substance of biological origin The chemiluminescence in the wavelength range is photoelectrically detected, a digital signal is generated, image processing is performed, and C
A chemiluminescence analysis system is also known in which a chemiluminescence image is displayed on a display means such as RT or a recording material such as a photographic film to obtain information on a substance of biological origin such as gene information.

Further, in recent years, cells, etc. have been
Viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA
A specific binding substance, such as A, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Forming a large number of independent spots, then cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c
A substance derived from a living body, such as DNA, DNA, or mRNA, which is collected from the living body by extraction or isolation, or which is further subjected to a chemical treatment, a chemical modification, or the like, such as a fluorescent substance or a dye. A substance labeled with a labeling substance is bound to a specific binding substance by hybridization or the like, to a microarray that is specifically bound,
A microarray analysis system has been developed which irradiates excitation light and photoelectrically detects light such as fluorescence emitted from a labeling substance such as a fluorescent substance or a dye to analyze a substance derived from a living body. According to this microarray analysis system,
A large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and the substance of biological origin labeled with the labeling substance is hybridized, thus In time, there is an advantage that it is possible to analyze a substance of biological origin.

In addition, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, RNAs, etc., derived from living organisms are located at different positions on the surface of a carrier such as a membrane filter. The specific binding substance that can specifically bind to the substance of which the base sequence, base length, composition, etc. are known is dropped using a spotter device to form a large number of independent spot-shaped regions. Formed and then cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs
For example, a substance derived from a living body that has been collected from a living body by extraction, isolation, or the like, or that has been further subjected to a chemical treatment, a chemical modification, etc. The macroarray specifically bound to the specific binding substance by hybridization or the like is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, thereby stimulating the stimulability. Exposing the phosphor layer to light, and then irradiating the stimulable phosphor layer with excitation light, photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to obtain data for biochemical analysis. A macroarray analysis system that uses a radiolabeled substance that generates and analyzes a substance derived from a living body has also been developed.

[0009]

The radiation image diagnostic system, the autoradiography analysis system, the fluorescence analysis system, the microarray analysis system and the macroarray analysis system all irradiate a sample with excitation light to stimulate photostimulation. Image data and light emission of the labeling substance by exciting the labeling substance such as the fluorescent substance or the fluorescent substance and photoelectrically detecting the photostimulated light emitted from the stimulable fluorescent substance and the fluorescence emitted from the fluorescent substance. Data generating devices for generating biochemical analysis data such as quantitative data are roughly divided into those using a scanner and those using a two-dimensional sensor. , In high resolution,
A scanner is used to generate the data.

In a radiation image diagnostic system, an autoradiography analysis system and a fluorescence analysis system,
In order to generate image data with high sensitivity using a scanner, it is irradiated with excitation light, and a stimulable phosphor sheet or
It is required to collect the photostimulable light or fluorescence emitted from the sample such as gel support or transfer support efficiently and photoelectrically, and in microarray analysis system and macroarray analysis system. In order to generate data for biochemical analysis with high sensitivity using a scanner, fluorescence emitted from samples such as a slide glass, a membrane filter, and a stimulable phosphor sheet is irradiated with excitation light. It is required to efficiently collect and stimulate photostimulable light and photoelectrically detect it.Therefore, the light omnidirectionally emitted from the sample is collected near the light emitting region of the sample. A scanner provided with an optical system that leads to an optical optical system has been proposed.

However, in these systems, the light emitted from the sample is omnidirectionally emitted by exciting the stimulable phosphor, fluorescent substance, etc. by the exciting light, and thus the exciting light is emitted. While securing the optical path of
In order to efficiently guide the light emitted from the irradiation region of the excitation light to the condensing optical system by using the optical system,
There is a problem that the structure of the optical system is inevitably complicated, which causes a cost increase.

Therefore, the present invention has a simple structure and efficiently excites omnidirectionally emitted light by exciting the stimulable phosphor or fluorescent substance contained in the sample by the excitation light. An object of the present invention is to provide a scanner that can collect light, generate high sensitivity, and generate data.

[0013]

The object of the present invention is to:
An excitation light source that emits excitation light, a sample stage on which a sample is placed, and a condensing optical system that condenses the light emitted from the sample by scanning the sample with the excitation light emitted from the excitation light source. A photodetector for photoelectrically detecting the light emitted from the sample and condensed by the condensing optical system, the sample stage, the optical path of the excitation light emitted from the excitation light source, and the condensing optics. Scanning means for relatively moving the system in a main scanning direction and a sub-scanning direction orthogonal to the main scanning direction; and a side of the condensing optical system, and of the sample irradiated with the excitation light. A reflection optical system that is provided in the vicinity of the region and that reflects the light emitted from the sample toward the sample is provided. Against over di-, the main scanning direction and the sub scanning direction is achieved by the scanner, characterized in that it is configured to be relatively moved.

According to the present invention, the scanner is provided on the side of the condensing optical system and in the vicinity of the region of the sample irradiated with the excitation light, and the light emitted from the sample is directed toward the sample. Since the reflection optical system is provided that is reflected and moved relative to the sample stage by the scanning means in the main scanning direction and the sub-scanning direction, the sample is emitted from the sample and condensed by the condensing optical system. The unreflected light can be reflected by the reflective optics to be incident in the vicinity of the area of the sample from which it was emitted, and thus the light reflected by the reflective optics and incident on the sample can be , The light is diffusely reflected in the vicinity of the emitted sample area, and at least a part of the light is collected by the collection optics and photoelectrically detected by the photodetector. The light emitted from the sample is reflected toward the sample on the side of the scientific system and in the vicinity of the region of the sample irradiated with the excitation light, and the main scanning is performed by the scanning means with respect to the sample stage. The stimulable phosphor or fluorescent substance contained in the sample is excited by the excitation light and emitted omnidirectionally only by providing the reflection optical system that is relatively moved in the scanning direction and the sub-scanning direction. It is possible to efficiently collect light, and photoelectrically detect it with a photodetector, and generate data with high sensitivity.

In a preferred aspect of the present invention, the scanning means includes the sample stage, an optical path of excitation light emitted from the excitation light source, and the condensing optical system.
A main scanning unit that moves intermittently relatively in the main scanning direction is included, and the reflection optical system reflects the light emitted from the sample toward the region of the sample from which the light is emitted. It is configured.

According to a preferred embodiment of the present invention, the scanning means moves the sample stage, the optical path of the excitation light emitted from the excitation light source and the focusing optical system relatively intermittently in the main scanning direction. The main scanning means for causing the reflective optics to reflect the light emitted from the sample towards the area of the sample from which the light was emitted, so that the light emitted from the same area of the sample The light collection efficiency can be significantly improved, and the light emitted omnidirectionally from the sample is efficiently collected and photoelectrically detected by the photodetector, with high sensitivity. It becomes possible to generate data.

In a preferred aspect of the present invention, the reflection optical system includes a reflection mirror.

In a further preferred aspect of the present invention, the reflection optical system guides the light emitted from the plurality of reflection mirrors and the sample to the reflection mirror, and the light is emitted from the sample. The sample is provided with a plurality of condenser lenses for condensing the light reflected by the sample.

[0019] In a further preferred aspect of the present invention, the reflective optical system includes a dichroic mirror having a property of transmitting light having a wavelength of excitation light and reflecting light emitted from the sample.

According to a further preferred embodiment of the present invention, the reflection optical system includes a dichroic mirror having a property of transmitting light having a wavelength of excitation light and reflecting light emitted from the sample. Even if the excitation light scattered by the sample together with the light emitted from the sample enters the reflection optical system, the excitation light is not reflected by the reflection optical system, and the excitation light is reflected by the reflection optical system. Therefore, it is possible to reliably prevent the photostimulable phosphor or the fluorescent substance contained in the sample from entering the sample and emitting the photostimulable light or fluorescence. Is generated, and only the light that is omnidirectionally emitted from the sample is efficiently collected and photoelectrically detected by the photodetector to generate data with high sensitivity. Rukoto becomes possible.

In a further preferred aspect of the present invention, the reflection optical system includes a spherical dichroic mirror having a property of transmitting light having a wavelength of excitation light and reflecting light emitted from the sample.

According to a further preferred embodiment of the present invention, the reflection optical system includes a spherical dichroic mirror having a property of transmitting light having a wavelength of excitation light and reflecting light emitted from the sample. Reflecting omnidirectionally emitted light from a sample and causing the light to enter the region of the sample from which the light is emitted, diffusely reflected by the sample, and at least a part thereof is condensed by a condensing optical system. On the other hand, even if the excitation light scattered by the sample enters the reflection optical system, the excitation light is not reflected by the reflection optical system, and the excitation light is reflected by the reflection optical system. Therefore, it becomes possible to surely prevent the photostimulable phosphor or the fluorescent substance contained in the sample from being incident on the sample and emitting the photostimulable light or fluorescence. It prevents noise from being generated in the data, efficiently collects only the light that is omnidirectionally emitted from the sample, and photoelectrically detects it with a photodetector for high sensitivity. Now it is possible to generate data.

[0023] In a further preferred aspect of the present invention, the reflective optical system transmits a light having a wavelength of excitation light and reflects a light emitted from the sample, and a plurality of dichroic mirrors, and the sample. Light is emitted from the sample to the dichroic mirror, and a plurality of condenser lenses that collect the light emitted from the sample and reflected by the dichroic mirror on the sample.

According to a further preferred embodiment of the present invention, the reflection optical system transmits a light having a wavelength of the excitation light and reflects a light emitted from the sample, and a plurality of dichroic mirrors emitted from the sample. In addition to guiding the reflected light to the dichroic mirror, the light emitted from the sample and reflected by the dichroic mirror is equipped with a plurality of condenser lenses for condensing the sample,
Light omnidirectionally emitted from the sample is reflected by a plurality of dichroic mirrors, is incident on the region of the sample from which the light is emitted, is diffusely reflected by the sample, and at least a part thereof is condensed optics. On the other hand, when the excitation light scattered by the sample is incident on the reflection optical system, the excitation light is not reflected by the reflection optical system, and the excitation light is It is possible to reliably prevent the photostimulable phosphor or fluorescent substance contained in the sample from being reflected by the reflection optical system and entering the sample to be excited to emit photostimulable light or fluorescence. Therefore, it is possible to prevent noise from being generated in the data, efficiently collect only the light that is omnidirectionally emitted from the sample, and photoelectrically detect it by the photodetector. High Time at, it is possible to generate the data.

In a preferred embodiment of the present invention, the sample is composed of a stimulable phosphor sheet having a stimulable phosphor layer containing a stimulable phosphor that has accumulated radiation energy.

In a further preferred aspect of the present invention, the stimulable phosphor layer of the stimulable phosphor sheet comprises:
A plurality of spot-shaped regions accumulating radiation energy are formed apart from each other.

[0027] In a further preferred aspect of the present invention, the stimulable phosphor layer of the stimulable phosphor sheet is
It is composed of a plurality of stimulable phosphor layer regions that are separated from each other, and the plurality of stimulable phosphor layer regions selectively store radiation energy.

[0028] In a preferred aspect of the present invention, the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet are formed on a support having a property of attenuating light energy and spaced from each other. The stimulable phosphor is filled and formed in the plurality of holes.

In a preferred embodiment of the present invention, the stimulable phosphor layer of the stimulable phosphor sheet has a plurality of holes formed in a support body having a property of attenuating light energy so as to be spaced apart from each other. In, the stimulable phosphor is filled, because it is composed of a plurality of stimulable phosphor layer regions formed, in each stimulable phosphor layer region, even if the excitation light is scattered, The scattered excitation light is further scattered in the support of the stimulable phosphor sheet and is incident on the adjacent stimulable phosphor layer regions, and is included in the adjacent stimulable phosphor layer regions. It becomes possible to effectively prevent the excited photostimulable phosphor from being excited, and thus the scattered excitation light is
After that, it becomes possible to effectively prevent the radiation energy accumulated by exciting the stimulable phosphor that is to be excited by the excitation light, in the form of stimulable light, and thus the accumulation. The stimulable phosphor contained in the stimulable phosphor layer of the stimulable phosphor sheet can be efficiently excited by excitation light, and with high resolution, the stimulable phosphor of the stimulable phosphor sheet It is possible to read the radiation data recorded in the layer.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet are formed separately from each other on a support having a property of attenuating light energy. In the multiple through holes
The photostimulable phosphor is embedded and formed.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet are formed separately from each other on a support having a property of attenuating light energy. In the multiple through holes
A stimulable phosphor film containing a stimulable phosphor is pressed and formed.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet are formed separately from each other on a support having a property of attenuating light energy. A stimulable phosphor is embedded and formed in the recesses thus formed.

In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet is formed with 10 or more stimulable phosphor layer regions.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is formed with 50 or more stimulable phosphor layer regions.

In a further preferred aspect of the present invention, 100 or more stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.

[0036] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is formed with 500 or more stimulable phosphor layer regions.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet has 1000
The above-described stimulable phosphor layer region is formed.

[0038] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is provided with 5000
The above-described stimulable phosphor layer region is formed.

[0039] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is provided with 1000
0 or more of the stimulable phosphor layer regions are formed.

[0040] In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is provided with 5000
0 or more of the stimulable phosphor layer regions are formed.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet has 1000
00 or more of the stimulable phosphor layer regions are formed.

In a preferred embodiment of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 5 mm 2.

[0043] In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are each formed on the support of the stimulable phosphor sheet in a size of less than 1 mm 2. .

In a further preferred aspect of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.5 mm 2. ing.

In a further preferred aspect of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.1 mm 2. ing.

In a further preferred aspect of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.05 mm 2. ing.

In a further preferred aspect of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.01 mm 2. ing.

In the present invention, the density of the stimulable phosphor layer region formed on the stimulable phosphor sheet is determined by the type of material of the support, the type of excitation light and the like.

[0049] In a preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed on the stimulable phosphor sheet at a density of 10 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in the stimulable phosphor sheet at a density of 50 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in the stimulable phosphor sheet at a density of 100 or more per cm 2.

[0052] In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in the stimulable phosphor sheet at a density of 500 pieces / square centimeter or more.

[0053] In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in the stimulable phosphor sheet at a density of 1,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed on the stimulable phosphor sheet at a density of 5000 pieces / square centimeter or more.

In a further preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in the stimulable phosphor sheet at a density of 10,000 or more per cm 2.

In a preferred aspect of the present invention, the plurality of stimulable phosphor layer regions are formed in a regular pattern on the support of the stimulable phosphor sheet.

In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet allows light to pass through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. At times, it is formed of a material having a property of attenuating the energy of light to 1/5 or less.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet allows light to pass through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. When you do, the energy of light is 1
It is formed of a material having a property of being attenuated to / 10 or less.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet allows light to pass through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. When you do, the energy of light is 1
It is formed of a material having a property of attenuating to less than / 50.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet allows light to pass through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. When you do, the energy of light is 1
It is made of a material having a property of damping to 100/100 or less.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet is such that light is transmitted through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. When you do, the energy of light is 1
It is formed of a material having a property of attenuating to / 500 or less.

In a further preferred aspect of the present invention, the support of the stimulable phosphor sheet allows light to pass through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. When you do, the energy of light is 1
It is formed of a material having a property of attenuating to 1000/1000 or less.

In the present invention, when the stimulable phosphor layer is composed of a plurality of stimulable phosphor layer regions, the material for forming the support of the stimulable phosphor sheet is
A material having a property of attenuating light energy is preferable, but the material is not particularly limited, and both an inorganic compound material and an organic compound material can be used,
Metal material, ceramic material or plastic material
Particularly preferably used.

In the present invention, when the stimulable phosphor layer is composed of a plurality of stimulable phosphor layer regions, an inorganic compound which can be preferably used for forming a support of the stimulable phosphor sheet. As the material, for example, gold,
Metals such as silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, Examples thereof include silicon materials such as silicon nitride; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, when the stimulable phosphor layer is composed of a plurality of stimulable phosphor layer regions, an organic compound material which can be used to form a support for the stimulable phosphor sheet. As the polymer compound, a polymer compound is preferably used, and examples thereof include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyfluoride. Vinylidene; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylons such as nylon 6, nylon 6,6 and nylon 4,10; polyimides; Risuruhon; phenolic resins such as novolak; silicon resins such as polydiphenylsiloxane; polyphenylene sulfide epoxy resin;
Polyurethane; polystyrene; butadiene-styrene copolymers; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan;
Urushi; polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds can be mentioned. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the greater the light scattering and / or absorption, the higher the light attenuating ability. Therefore, when the stimulable phosphor layer is composed of a plurality of stimulable phosphor layer regions, the storage property is increased. The support of the phosphor sheet preferably has an absorbance of 0.3 or more per 1 cm of thickness, and a thickness of 1 c.
More preferably, the absorbance per m is 1 or more.
Here, the absorbance is calculated by calculating the A / T by placing an integrating sphere immediately after the plate having a thickness of Tcm and measuring the transmitted light amount A at the wavelength of the probe light or the emission light used for the measurement with a spectrophotometer. By being asked. In order to improve the light attenuating ability, a light scatterer or a light absorber can be contained in the support of the stimulable phosphor sheet.
Fine particles of a material different from the material forming the support of the stimulable phosphor sheet are used as the light scatterer, and pigments or dyes are used as the light absorber.

In another preferred embodiment of the present invention, the stimulable phosphor layer of the stimulable phosphor sheet is
It is formed uniformly on the surface of the support.

In the present invention, the stimulable phosphor contained in the stimulable phosphor layer is capable of accumulating radiation energy, is excited by electromagnetic waves, and releases the accumulated radiation energy in the form of light. There is no particular limitation as long as it is possible, but one capable of being excited by light in the visible light wavelength range is preferable. Specifically, for example, alkaline earth metal fluorohalide-based phosphors (Ba1-xM) disclosed in U.S. Pat. No. 4,239,968 are disclosed.
²⁺ x) FX: yA (where M ²⁺ is Mg, Ca, S
at least one alkaline earth metal element selected from the group consisting of r, Zn and Cd, X is Cl, Br and I
At least one halogen selected from the group consisting of A
Is Eu, Tb, Ce, Tm, Dy, Pr, Ho, Nd,
At least one trivalent metal element selected from the group consisting of Yb and Er, x is 0 ≦ x ≦ 0.6, and y is 0 ≦ y ≦
It is 0.2. ), The alkaline earth metal fluorohalide-based phosphor S disclosed in JP-A-2-276997.
rFX: Z (where X is at least one halogen selected from the group consisting of Cl, Br and I, and Z is Eu or Ce), and europium activation disclosed in JP-A-59-56479. Composite halogen-based phosphor B
aFX · xNaX ′: aEu ²⁺ (wherein X and X ′ are at least one halogen selected from the group consisting of Cl, Br, and I, and x is 0 <x ≦
2, a is 0 <a ≦ 0.2. ), JP-A-58-69
MOX: xCe, which is a cerium-activated trivalent metal oxyhalogen-based phosphor disclosed in Japanese Patent No. 281
Is Pr, Nd, Pm, Sm, Eu, Tb, Dy, Ho,
At least one trivalent metal element selected from the group consisting of Er, Tm, Yb, and Bi, X is one or both of Br and I, and x is 0 <x <0.1. ), L which is a cerium-activated rare earth oxyhalogen-based phosphor disclosed in US Pat. No. 4,539,137.
nOX: xCe (where Ln is at least one rare earth element selected from the group consisting of Y, La, Gd and Lu, X is at least one halogen selected from the group consisting of Cl, Br and I, and x is 0 <X ≦ 0.1) and the europium-activated composite halogen-based phosphor M ^II FX disclosed in US Pat. No. 4,962,047.
・ AM ^I X '・ bM' ^II X ^" 2 ・ cM ^III X ^'" 3 ・ x
A: yEu ²⁺ (where M ^II is Ba, Sr and Ca
At least one alkaline earth metal element selected from the group consisting of, M ^I is at least one alkali metal element selected from the group consisting of Li, Na, K, Rb and Cs, and M ′ ^II is a group consisting of Be and Mg. more least one trivalent metal element selected, M ^III is Al, Ga, I
At least one trivalent metal element selected from the group consisting of n and Tl, A is at least one metal oxide, X is at least one halogen selected from the group consisting of Cl, Br and I, X ′, X ^″ And X ^''' is F, Cl,
It is at least one halogen selected from the group consisting of Br and I, a is 0 ≦ a ≦ 2, b is 0 ≦ b ≦ 1
0 ⁻² and c are 0 ≦ c ≦ 10 ⁻² , and a + b + c
≧ 10 ⁻² , x is 0 <x ≦ 0.5, and y is 0.
<Y ≦ 0.2. ) Can be preferably used.

[0069] In a preferred aspect of the present invention, the sample is constituted by a biochemical analysis unit in which a plurality of regions containing a fluorescent substance are formed apart from each other.

[0070] In a further preferred aspect of the present invention, the biochemical analysis unit is provided with a plurality of lane-shaped regions containing a fluorescent substance, which are separated from each other.

In a further preferred aspect of the present invention, the biochemical analysis unit includes an absorptive substrate formed of an absorptive material, and the plurality of lane-shaped regions containing a fluorescent substance are the absorptive substrate. Are formed separately from each other.

In another preferred embodiment of the present invention, the biochemical analysis unit includes an absorptive substrate formed of an absorptive material, and the plurality of spot-like absorptive regions containing a fluorescent substance are The adsorbent substrate is formed so as to be separated from each other.

In another preferred embodiment of the present invention, the biochemical analysis unit has a substrate having a property of attenuating the energy of light, and a plurality of spot-shaped adsorptions formed on the substrate so as to be separated from each other. And a plurality of absorptive regions selectively containing a fluorescent substance.

[0074] In a preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are provided in a plurality of holes formed in a substrate having a property of attenuating light energy so as to be separated from each other. Is formed by being filled with an adsorbent material.

According to a preferred embodiment of the present invention, the plurality of absorptive regions of the biochemical analysis unit are provided in a plurality of holes formed at a distance from each other on a substrate having a property of attenuating light energy. Since the adsorbent material is filled and formed, even if the excitation light is scattered in each adsorptive region, the scattered excitation light is further scattered in the substrate of the biochemical analysis unit. Therefore, it is possible to effectively prevent the fluorescent substance contained in the adjacent absorptive regions from being excited by being incident on the adjacent absorptive regions, and therefore, due to the scattered excitation light, Since it is possible to effectively prevent the fluorescent substance that should not be excited from being excited and emit fluorescence, the fluorescence recorded in the multiple absorptive regions of the biochemical analysis unit can be effectively resolved. Read data It becomes possible.

In a further preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are provided on a substrate having a property of attenuating light energy.
An adsorptive material is embedded in a plurality of through-holes formed so as to be separated from each other.

In another preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are provided on a substrate having a property of attenuating light energy.
An adsorptive film containing an adsorptive material is press-fitted into a plurality of through-holes formed so as to be separated from each other.

In a further preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are provided on a substrate having a property of attenuating light energy.
An adsorbent material is embedded and formed in a plurality of recesses formed so as to be separated from each other.

In a preferred aspect of the present invention, 10 or more absorptive regions are formed on the substrate of the biochemical analysis unit.

[0080] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 50 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 100 or more absorptive regions.

[0082] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 500 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
The absorptive region described above is formed.

[0084] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 5000
The absorptive region described above is formed.

[0085] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
Zero or more of the absorptive regions are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 5000
Zero or more of the absorptive regions are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
00 or more of the absorptive regions are formed.

In a preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 0.1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 0.05 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed on the substrate of the biochemical analysis unit in a size of less than 0.01 mm 2.

In the present invention, the density of the absorptive region formed in the biochemical analysis unit is determined by the type of substrate material and the type of excitation light.

[0095] In a preferred aspect of the present invention, the plurality of absorptive regions are provided on the substrate of the biochemical analysis unit at a density of 10 or more per cm 2.
Has been formed.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 50 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 100 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 500 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate of the biochemical analysis unit at a density of 1,000 or more per cm 2.

[0100] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 5,000 or more per cm 2.

[0101] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the substrate of the biochemical analysis unit at a density of 10,000 or more per cm 2.

In a preferred aspect of the present invention, the plurality of absorptive regions are formed in a regular pattern on the substrate of the biochemical analysis unit.

[0103] In a preferred aspect of the present invention, when the substrate of the biochemical analysis unit has a distance equal to the distance between adjacent absorptive regions, the light is transmitted through the substrate. It is formed of a material having a property of attenuating energy to 1/5 or less.

In a further preferred aspect of the present invention, when light is transmitted through the substrate by a distance equal to a distance between adjacent absorptive regions of the substrate of the biochemical analysis unit, Is formed of a material having a property of attenuating the energy of 1/10 or less.

[0105] In a further preferred aspect of the present invention, when light is transmitted through the substrate by a distance equal to a distance between adjacent absorptive regions of the substrate of the biochemical analysis unit, Is formed of a material having a property of attenuating the energy of 1/50 or less.

In a further preferred aspect of the present invention, when light is transmitted through the substrate by a distance equal to a distance between adjacent absorptive regions of the substrate of the biochemical analysis unit, Is formed of a material having a property of attenuating the energy of 1/100 or less.

[0107] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light in the substrate by a distance equal to a distance between adjacent absorptive regions, Is formed of a material having a property of attenuating the energy of 1/500 or less.

[0108] In a further preferred aspect of the present invention, when light is transmitted through the substrate by a distance equal to a distance between adjacent absorptive regions of the substrate of the biochemical analysis unit, Energy of 1/1000
It is formed of a material having the following damping property.

In the present invention, when a plurality of absorptive regions are formed on the substrate of the biochemical analysis unit, the material for forming the substrate of the biochemical analysis unit has a property of attenuating light energy. Although it is preferable that the compound has an inorganic compound material,
Any organic compound material can be used, with metallic, ceramic or plastic materials being particularly preferred.

In the present invention, when a plurality of absorptive regions are formed on the substrate of the biochemical analysis unit, the inorganic compound material that can be preferably used for forming the substrate of the biochemical analysis unit is For example, gold,
Metals such as silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, Examples thereof include silicon materials such as silicon nitride; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, when a plurality of absorptive regions are formed on the substrate of the biochemical analysis unit, as an organic compound material that can be used to form the substrate of the biochemical analysis unit, Molecular compounds are preferably used, for example, polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride;
Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, nylon 6,6, nylon 4, 10 etc. nylon; Polyimide; Polysulfone Polyphenylene sulfide; silicon resin such as polydiphenyl siloxane;
Phenolic resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer; cellulose, cellulose acetate, nitrocellulose,
Examples include polysaccharides such as starch, calcium alginate, and hydroxypropylmethyl cellulose; chitin; chitosan; sumacum; polyamides such as gelatin, collagen, and keratin, and copolymers of these polymer compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the greater the scattering and / or absorption of light, the higher the light attenuating ability. Therefore, when a plurality of absorptive regions are formed on the substrate of the biochemical analysis unit,
The substrate of the biochemical analysis unit preferably has an absorbance of 0.3 or more per cm of thickness, and more preferably an absorbance of 1 or more per cm of thickness. Here, the absorbance is calculated by calculating the A / T by placing an integrating sphere immediately after the plate having a thickness of Tcm and measuring the transmitted light amount A at the wavelength of the probe light or the emission light used for the measurement with a spectrophotometer. By being asked. In order to improve the light attenuating ability, a light scatterer or a light absorber may be contained in the substrate of the biochemical analysis unit. Fine particles of a material different from the material forming the substrate of the biochemical analysis unit are used as the light scatterer, and pigments or dyes are used as the light absorber.

In a preferred embodiment of the present invention, the excitation light source is a laser excitation light source which emits laser light.

[0114]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit.

As shown in FIG. 1, the biochemical analysis unit 1 is provided with a substrate 2 formed of stainless steel and having a large number of substantially circular through holes 3 formed therein. Nylon 6 is filled inside the through hole 3 to form a large number of absorptive regions 4 in a dot shape.

Although not shown exactly in FIG. 1, in the present embodiment, the substantially circular through holes 3 having a size of about 0.07 mm 2 are regularly arranged in a matrix of 120 columns × 160 rows. Is formed on the substrate 2,
Therefore, a total of 19200 absorptive regions 4 are formed. The absorptive region 4 is formed by filling a large number of through holes 3 with nylon 6 so that the surface thereof is located at the same height as the surface of the substrate 2.

In the biochemical analysis, in a large number of absorptive regions 4 regularly formed in the biochemical analysis unit 1, for example, as a specific binding substance, a plurality of cDNAs having different base sequences and different cDNAs are known. The solution containing is dropped using a spotting device, and the specific binding substance is fixed in the large number of absorptive regions 4 of the biochemical analysis unit 1.

FIG. 2 is a schematic front view of the spotting device.

As shown in FIG. 2, the spotting device comprises an injector 5 for injecting a solution containing a specific binding substance toward the surface of the biochemical analysis unit 1 and a CCD.
With the camera 6, the tip of the injector 5 is observed while observing the tip of the injector 5 and the absorptive region 4 of the biochemical analysis unit 1 on which the solution containing the specific binding substance should be dropped by the CCD camera 6. When the center of the adsorptive region 4 to which the solution containing the specific binding substance is to be dropped coincides, the solution containing the specific binding substance such as plural cDNAs having different base sequences from the injectors 5 is detected. It is configured to be dropped, and it is guaranteed that the solution containing the specific binding substance can be dropped accurately into the large number of dot-shaped absorptive regions 4 of the biochemical analysis unit 1. .

Then, in this way, the specific binding substance adsorbed on the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is selectively selected from the substances of biological origin labeled with the labeling substance. To be hybridized.

FIG. 3 is a schematic vertical sectional view of a hybridization reaction container.

As shown in FIG. 3, the hybridization reaction container 8 has a rectangular cross section, and a hybridization reaction solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance, is housed therein. ing.

When selectively binding a specific binding substance such as cDNA with a radioactive labeling substance, a hybridization reaction solution 9 containing a substance derived from a living body which is a probe labeled with the radioactive labeling substance is prepared, It is housed in the hybridization reaction container 8.

On the other hand, by using a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate, the cDNA is
In the case of selectively labeling a specific binding substance such as, a reaction hybridization solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, is used. It is prepared and housed in the hybridization reaction container 8.

Furthermore, in the case of selectively labeling a specific binding substance such as cDNA with a fluorescent substance such as a fluorescent dye, a high-molecular substance containing a probe which is a probe labeled with a fluorescent substance such as a fluorescent dye is used. A hybridization reaction solution 9 is prepared and housed in the hybridization reaction container 8.

[0127] A substance derived from a living body labeled with a radioactive labeling substance, a substance derived from a living body labeled with a labeling substance that produces chemiluminescence by contacting with a chemiluminescent substrate, and a living body labeled with a fluorescent substance such as a fluorescent dye It is also possible to prepare a hybridization reaction solution 9 containing two or more substances derived from a living body among the substances derived from the living body and store it in the hybridization reaction container 8. In this embodiment, the hybridization reaction solution 9 is labeled with a radioactive labeling substance. The hybridization reaction solution 9 containing the biological substance and the biological substance labeled with a fluorescent substance such as a fluorescent dye is prepared and housed in the hybridization reaction container 8.

For hybridization, cD
The biochemical analysis unit 1 in which a specific binding substance such as NA is immobilized on a large number of adsorptive regions 4 is inserted into the hybridization reaction container 8 and vibrated in the hybridization reaction container 8 for a predetermined time. Is added.

As a result, the biogenic substance labeled with a radioactive labeling substance and the biogenic substance labeled with a fluorescent substance such as a fluorescent dye are adsorbed to a large number of absorptive regions 4 by convection or diffusion. The specific binding substance is contacted and selectively hybridized.

The biogenic substance labeled with the radioactive labeling substance and the biogenic substance labeled with the fluorescent substance are contained in a large number of absorptive regions 4 of the biochemical analysis unit 1.
Selectively hybridized to the specific binding substance contained in the hybridization reaction container 8,
The hybridization reaction solution 9 is discharged, the cleaning solution is supplied to the hybridization reaction container 8, and the biochemical analysis unit 1 is cleaned.

In the present embodiment, since the biochemical analysis unit 1 is formed by filling nylon 6 into a large number of through holes 3 formed in the stainless steel substrate 2, hybridization is performed. Almost no expansion or contraction even when subjected to liquid treatment such as washing and washing.

In this way, the radiation data of the radioactive labeling substance as the labeling substance and the fluorescence data of the fluorescent substance such as the fluorescent dye are recorded in the large number of absorptive regions 4 of the biochemical analysis unit 1. The fluorescence data recorded in the absorptive region 4 is read by a scanner described later, and biochemical analysis data is generated.

On the other hand, the radiation data of the radiolabeled substance is
The radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by a scanner described later to generate biochemical analysis data.

FIG. 4 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 4, the stimulable phosphor sheet 10 according to this embodiment has a large number of substantially circular through holes 1.
3 is provided with a support 11 made of stainless steel regularly formed, and in a large number of through holes 13 formed in the support 11,
The stimulable phosphor is embedded, and a large number of stimulable phosphor layer regions 12 are formed in a dot shape.

The large number of through holes 13 are formed in the support 11 in the same pattern as the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and each of the biochemical analysis unit 1 is formed. It has the same size as the large number of absorptive regions 4 formed on the substrate 2.

Therefore, although not exactly shown in FIG. 4, in the present embodiment, the substantially circular stimulable phosphor layer region 1 having a size of about 0.07 mm 2 is used.
2 are formed on the support 11 in a matrix of 120 columns × 160 rows, so that a total of 19,200 stimulable phosphor layer regions 12 are formed in dots.

Although not shown, in the present embodiment, the support 1 is arranged so that the surface of the support 11 and the surface of each stimulable phosphor layer region 12 are located at the same height.
The stimulable phosphor is embedded in the through-hole 13 formed in No. 1 to form each stimulable phosphor layer region 12.

FIG. 5 shows the substrate 2 of the biochemical analysis unit 1.
A large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 by the radioactive labeling substance selectively contained in the large number of absorptive regions 4 formed in the.
FIG. 6 is a schematic cross-sectional view showing a method of exposing a substrate.

As shown in FIG. 5, the radioactive labeling substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 causes the stimulable phosphor sheet 10 to be removed. When exposing the large number of stimulable phosphor layer regions 12 formed on the support 11, the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are exposed. The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are overlapped with each other so as to face the corresponding large number of absorptive regions 4 formed in the chemical analysis unit 1.

In the present embodiment, the biochemical analysis unit 1 is formed by filling nylon 6 into a large number of through holes 3 formed in the stainless steel substrate 2, so that the hybridization is performed. As described above, there is almost no expansion and contraction even when subjected to a treatment with a liquid, and therefore, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are each biochemically analyzed. Of the stimulable phosphor sheet 1 so as to exactly face the corresponding absorptive region 4 formed on the substrate 2 of the unit 1 for application
0 and the biochemical analysis unit 1 can be easily and surely overlapped to expose the stimulable phosphor layer region 12.

Thus, over a predetermined period of time, each stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 corresponds to the substrate 2 of the biochemical analysis unit 1 corresponding thereto. By supporting the absorptive region 4 by facing, the stimulable phosphor sheet 10 is supported by the radioactive labeling substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. A large number of stimulable phosphor layer regions 12 formed on the body 11 are exposed.

At this time, from the radiolabeled substance contained in the adsorptive region 4 of the biochemical analysis unit 1, an electron beam (β
Lines are emitted, a large number of absorptive regions 4 of the biochemical analysis unit 1 are formed on the substrate 2 formed of stainless steel so as to be spaced apart from each other, and around each absorptive region 4, Since the stainless steel substrate 2 having the property of attenuating the radiation energy is present, the electron beam (β-ray) emitted from the radiolabeled substance contained in the adsorptive region 4 of the biochemical analysis unit 1 However, it is possible to effectively prevent scattering in the substrate 2 of the biochemical analysis unit 1, and moreover, a large number of stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 are made of stainless steel. A large number of through holes 13 that are formed in the support 11 made of metal and are spaced apart from each other.
A support 11 formed by embedding a stimulable phosphor therein and formed of stainless steel having a property of attenuating radiation energy is present around each stimulable phosphor layer region 12. Biochemical analysis unit 1
It is possible to effectively prevent the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive region 4 of (3) in the support 11 of the stimulable phosphor sheet 10 from being scattered. Therefore, the stimulable phosphor layer region facing the absorptive region 4 by the electron beam (β-ray) emitted from the radiolabel substance contained in each absorptive region 4 of the biochemical analysis unit 1. 12 can be selectively exposed.

Thus, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are
Radiation data of radiolabeled substances are recorded.

FIG. 6 shows the radiation data of the radiolabeled substance recorded in a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the biochemical analysis unit 1. FIG. 7 is a schematic perspective view of a scanner that reads fluorescence data such as a fluorescent dye recorded in a large number of absorptive regions 4 formed on the substrate 2 to generate biochemical analysis data. FIG. 7 is a photomultiplier. It is a schematic perspective view which shows the detail of the scanner of the vicinity.

The scanner according to this embodiment is provided with radiation data and biochemical analysis of radiolabeled substances recorded in a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. The fluorescent data such as the fluorescent dye recorded in the large number of absorptive regions 4 formed on the substrate 2 of the unit 1 for reading is configured to be readable.
A first laser pumping light source 21 that emits a laser light 24 having a wavelength of 40 nm, a second laser pumping light source 22 that emits a laser light 24 having a wavelength of 532 nm, and a third laser pumping light that emits a laser light 24 having a wavelength of 473 nm. And a light source 23.

In the present embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source, and the second laser excitation light source 22 and the third laser excitation light source 23.
Is composed of a second harmonic generation element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. The first dichroic mirror 27 and 532 nm that transmit the laser light 4 of 640 nm and reflect the light of wavelength 532 nm are transmitted through the optical path of the laser light 24 emitted from the first laser excitation light source 21 and reflected by the mirror 26. A second dichroic mirror 28 that transmits light of the above wavelength and reflects light of the wavelength of 473 nm is provided, and the laser light 24 generated by the first laser excitation light source 21 is provided.
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated from the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, the first dichroic mirror 27
Is reflected by the second dichroic mirror 28, the direction of which is changed by 90 degrees, the light is transmitted through the second dichroic mirror 28, and is incident on the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31 and then reflected by the second dichroic mirror 28 so that its direction is 90 degrees. After being changed, it is incident on the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 33 formed by a concave mirror having a hole 33a formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33a of the perforated mirror 33. Pass through the concave mirror 34
Incident on.

The laser light 24 incident on the concave mirror 34
Is reflected by the concave mirror 34, and the optical head 3
It is incident on 5.

FIG. 8 is a schematic sectional view of the optical head 35, and FIG. 9 is a schematic bottom view thereof.

As shown in FIGS. 8 and 9, the optical head 35 includes a mirror 36 and an aspherical lens 37, and the laser light 24 incident on the optical head 35 is reflected by the mirror 36. The light is incident on the aspherical lens 37 and is incident on the stimulable phosphor sheet 10 mounted on the glass plate 41 of the stage 40 or the biochemical analysis unit 1 by the aspherical lens 37.

As shown in FIGS. 8 and 9, the optical head 35 further includes the stimulable phosphor sheet 10 or the biochemical analysis unit 1 to be irradiated with the laser light 34.
The four condensing lenses 38a, 38b, 3 are provided on the side of the region of
8c, 38d and four dichroic mirrors 39a,
It is provided with 39b, 39c and 39d.

In this embodiment, four types of optical heads 35 having dichroic mirrors 39a, 39b, 39c and 39d having different characteristics are prepared, and predetermined optical elements are used depending on the labeling substance and laser excitation light source used. The head 35 is configured to be attached to the scanner.

The first optical head 35 uses the first laser excitation light source 21 and uses the substrate 2 of the biochemical analysis unit 1.
The dichroic mirror 39 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Each of a, 39b, 39c, and 39d has a property of transmitting light having a wavelength of 640 nm and reflecting light having a wavelength longer than 640 nm.

The second optical head 35 uses the second laser excitation light source 22 and uses the substrate 2 of the biochemical analysis unit 1.
The dichroic mirror 39 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Each of a, 39b, 39c, and 39d has a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm.

The third optical head 35 uses the third laser excitation light source 23 and uses the substrate 2 of the biochemical analysis unit 1.
The dichroic mirror 39 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Each of a, 39b, 39c, and 39d has a property of transmitting light having a wavelength of 473 nm and reflecting light having a wavelength longer than 473 nm.

The fourth optical head 35 is included in a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 by using the first laser excitation light source 21. The dichroic mirrors 39a, 39 are used to excite the stimulable phosphor existing therein and read the stimulable light 45 emitted from the stimulable phosphor layer region 12.
b, 39c, and 39d have the property of reflecting only the light in the wavelength region of the photostimulable light 45 and transmitting the light of the wavelength of 640 nm.

When the laser beam 24 enters the stimulable phosphor layer area 12 of the stimulable phosphor sheet 10, the stimulable phosphor layer area 12 formed on the support 11 of the stimulable phosphor sheet 10 is irradiated. The photostimulable phosphor contained therein is excited, and photostimulable light 45 is emitted omnidirectionally.

The photostimulable light 45 emitted from the photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 and incident on the aspherical lens 37 of the fourth optical head 35 is aspherical lens 37.
Is focused on the mirror 36, reflected by the mirror 36 on the same side as the optical path of the laser light 24, and made into parallel light, which then enters the concave mirror 34.

On the other hand, the aspherical lens 3 is emitted from the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10.
7. Condensing lenses 38a, 38b, 38 arranged on the side of 7
The photostimulable rays 45 incident on c and 38d are condensed on dichroic mirrors 39a, 39b, 39c and 39d, respectively.

Here, the dichroic mirrors 39a, 39b, 39c, 39d of the fourth optical head 35 have the property of reflecting only the light in the wavelength region of the stimulating light 45 and transmitting the light of the wavelength of 640 nm. Therefore, the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is dichroic mirrors 39a, 39b, 39.
The stimulable phosphor layer region 1 of the stimulable phosphor sheet 10 in which the stimulable light 45 is emitted by being reflected by c and 39d.
A part of the photostimulable light 45 that is incident on the light beam 2, is diffusely reflected, and is diffusely reflected is incident on the aspherical lens 37.
Is focused on the mirror 36, reflected by the mirror 36 on the same side as the optical path of the laser light 24, and made into parallel light, which then enters the concave mirror 34.

On the other hand, the condenser lens 38 is diffusely reflected by the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10.
The photostimulable light 45 incident on a, 38b, 38c, and 38d is
Dichroic mirrors 39a, 39b, 39, respectively
In the same manner, the stimulable light 45 is condensed back to the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 from which the stimulable light 45 has been emitted, is diffusely reflected, and is diffusely reflected. A part of 45 is incident on the aspherical lens 37.

While the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is irradiated with the laser beam 24, it is emitted from the stimulable phosphor layer region 12 and is on the side of the aspherical lens 37. One condensing lens 38a, 38b, 38c, 38
Dichroic mirror 39 of photostimulable light 45 incident on d
Reflection by a, 39b, 39c, 39d, and its stimulated emission 4
Diffuse reflection is repeated by the photostimulable phosphor layer region 12 in which 5 is emitted, and a part of the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 toward the side of the aspherical lens 37. Enters the aspherical lens 37 and is focused on the mirror 36 by the aspherical lens 37.

On the other hand, together with the stimulated emission light 45, the condenser lenses 38a, 38b, 3 arranged laterally of the aspherical lens 37.
8c and 38d, and the dichroic mirror 39a,
The laser light 24 focused on 39b, 39c, 39d is
Since the dichroic mirrors 39a, 39b, 39c, 39d provided in the fourth optical head 35 have a property of transmitting light having a wavelength of 640 nm, the dichroic mirrors 39a, 39b, 39c, 39d are transmitted, Therefore, the laser light 24 is sent back to the stimulable phosphor layer region 12 containing the excited stimulable phosphor, and the stimulable phosphor that has already emitted the stimulable light 45 is repeatedly excited, The photostimulable phosphor layer region 1 which emits photostimulable light 45
It is possible to reliably prevent the amount of the stimulated stimulus light 45 emitted from 2 from becoming excessive.

When the laser beam 24 is incident on the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1, a fluorescent substance such as a fluorescent dye contained in the absorptive region 4 is excited. , The fluorescence 45 is omnidirectionally emitted.

The aspherical lens 37 of the first optical head 35, the second optical head 35 or the third optical head 35 is emitted from the absorptive region 4 of the biochemical analysis unit 1.
The fluorescence 45 that has entered the laser beam is focused on the mirror 36 by the aspherical lens 37, and the laser beam 2 is reflected by the mirror 36.
The light is reflected to the same side as the optical path of No. 4, and is made into parallel light, and then enters the concave mirror 34.

On the other hand, condensing lenses 38a, 38b, 38c, 38d which are emitted from the absorptive region 4 of the biochemical analysis unit 1 and are arranged on the side of the aspherical lens 37.
The fluorescent light 45 that has been incident on is collected on the dichroic mirrors 39a, 39b, 39c, and 39d, respectively.

Here, the dichroic mirrors 39a, 39b, 39c, 39d of the first optical head 35 are 64
The dichroic mirrors 39a, 39b, 39c, 39d of the second optical head 35 have a property of transmitting light having a wavelength of 0 nm and reflecting light having a wavelength longer than 640 nm.
Has a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm, and the dichroic mirrors 39a, 39b, 39c of the third optical head 35,
39d transmits light having a wavelength of 473 nm and transmits light having a wavelength of 473 nm.
Because it has the property of reflecting light with a longer wavelength than
The fluorescence 45 emitted from the adsorptive region 4 of the biochemical analysis unit 1 is dichroic mirrors 39a, 39b, 3 and 3.
The fluorescence 45 reflected by 9c and 39d enters the absorptive region 4 of the biochemical analysis unit 1 from which the fluorescence 45 has been emitted, and a part of the fluorescence 45 diffusely reflected and diffusely reflected enters the aspherical lens 37. Then, by the aspherical lens 37,
The light is focused on the mirror 36, is reflected by the mirror 36 on the same side as the optical path of the laser light 24, is made into parallel light, and is incident on the concave mirror 34.

On the other hand, the absorptive region 4 of the biochemical analysis unit 1 causes diffuse reflection, and the condenser lenses 38a, 38
Fluorescent light 45 that has entered b, 38c, and 38d,
Dichroic mirrors 39a, 39b, 39c, 39d
Part of the fluorescence 45 that is diffused and diffused and reflected by the absorptive region 4 of the biochemical analysis unit 1 from which the fluorescence 45 is collected is similarly reflected by the aspherical lens 37. Incident.

While the absorptive region 4 of the biochemical analysis unit 1 is being irradiated with the laser beam 24, the absorptive region 4 is emitted from the absorptive region 4 and the condenser lenses 38a, 38b on the side of the aspherical lens 37. , 38c, 38d incident fluorescence 45
Dichroic mirrors 39a, 39b, 39c, 39
The absorptive region 4 where the fluorescence 45 is emitted by the reflection by d
Diffuse reflection is repeated, and a part of the fluorescent light 45 emitted from the absorptive region 4 to the side of the aspherical lens 37 enters the aspherical lens 37, and is reflected by the aspherical lens 37 to the mirror 36. Collected.

On the other hand, together with the fluorescent light 45, the aspherical lens 3
7. Condensing lenses 38a, 38b, 38 arranged on the side of 7
c, 38d and enters the dichroic mirrors 39a, 3d.
The dichroic mirrors 39a, 39b, 39c, 39d provided on the first optical head 35 have a property of transmitting the laser light 24 focused on 9b, 39c, 39d and having a wavelength of 640 nm. Dichroic mirrors 39a, 39b, 39 provided on the second optical head 35.
c and 39d have a property of transmitting light having a wavelength of 532 nm, and the dichroic mirrors 39a, 39b, 39c, and 39d provided in the third optical head 35 are 473n.
Since it has a property of transmitting light having a wavelength of m, it transmits through the dichroic mirrors 39a, 39b, 39c, 39d, and therefore the laser light 24 is sent back to the absorptive region 4 containing the excited fluorescent substance. It is possible to reliably prevent the fluorescent substance that has already emitted the fluorescent light 45 from being repeatedly excited to emit the fluorescent light 45 and to cause the amount of the fluorescent light 45 emitted from the adsorptive region 4 to become excessive. .

The photostimulable light 45 or fluorescent light 45 that has entered the concave mirror 34 is reflected by the concave mirror 34,
The light enters the perforated mirror 33.

The photostimulable light 45 or the fluorescence 45 incident on the perforated mirror 33 is reflected downward by the perforated mirror 33 formed by a concave mirror to enter the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 7, the filter unit 48 includes four filter members 51a, 51b, 51c,
51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 8 by a motor (not shown).

FIG. 10 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 10, the filter member 5
1a includes a filter 52a, and the filter 52a includes a first
The laser excitation light source 21 is used to excite a fluorescent substance such as a fluorescent dye contained in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 and used when reading the fluorescence 45. 640nm which is a filter member
It has a property of cutting off light having a wavelength of, and transmitting light having a wavelength longer than 640 nm.

FIG. 11 is a sectional view taken along the line BB of FIG.

As shown in FIG. 11, the filter member 5
1b includes a filter 52b, and the filter 52b includes a second
Used when the fluorescent substance such as a fluorescent dye contained in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is excited by using the laser excitation light source 22 of FIG. 532nm which is a filter member
It has a property of cutting off light having a wavelength of, and transmitting light having a wavelength longer than 532 nm.

FIG. 12 is a sectional view taken along the line CC of FIG.

As shown in FIG. 12, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
When a fluorescent substance such as a fluorescent dye contained in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is excited by using the laser excitation light source 23 of FIG. It is a filter member used, 473n
It has a property of cutting light having a wavelength of m and transmitting light having a wavelength longer than 473 nm.

FIG. 13 is a sectional view taken along the line DD of FIG.

As shown in FIG. 13, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
No. 45 of the stimulable phosphor layer region 12 formed on the support 11 of 0. A stimulable phosphor layer region 1 which is a filter used when reading
2 has a property of transmitting only the light in the wavelength region of the stimulated emission light 45 emitted from No. 2 and cutting the light of the wavelength of 640 nm.

Therefore, the filter members 51a, 51b, 51c, 51d are selected depending on the laser excitation light source to be used.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processing device 54.

Although not shown in FIG. 6, the optical head 35 is configured to be movable in the main scanning direction and the sub scanning direction orthogonal to the main scanning direction by the scanning mechanism, and supports the stimulable phosphor sheet 10. All the stimulable phosphor layer regions 12 formed on the body 11 and all the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are configured to be scanned by the laser light 24. There is.

FIG. 14 is a schematic plan view of the scanning mechanism of the optical head.

In FIG. 14, for simplification, the optical system excluding the optical head 35 and the optical paths of the laser light 24 and the fluorescent light 45 or the stimulated light 45 are omitted.

As shown in FIG. 14, the optical head 35.
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
14 are fixed, and the substrate 6 which is movable on the substrate 60 in the sub-scanning direction indicated by the arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in the hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63. The main scanning stepping motor 65 connects the endless belt 66 to the adjacent through holes 3 formed in the biochemical analysis unit 1, that is, the adjacent through holes 3. The stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 can be driven intermittently at a pitch equal to the distance between adjacent stimulable phosphor layer regions 12.

The optical head 35 includes an endless belt 66.
When the endless belt 66 is driven by the main scanning stepping motor 65, the endless belt 66 is moved in the main scanning direction indicated by the arrow X in FIG.

In FIG. 14, 67 is the optical head 35.
Is a linear encoder for detecting the position in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is intermittently driven in the main scanning direction, and when scanning of one line is completed, the substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61. The optical head 35 is
In FIG. 14, all the stimulable fluorescence formed on the support 11 of the stimulable phosphor sheet 10 by the laser light 24 moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y. The body layer region 12 or all absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned.

FIG. 15 is a block diagram showing the control system, input system, drive system and detection system of the scanner according to the preferred embodiment of the present invention.

As shown in FIG. 15, the control system of the scanner is a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 which is operated by the user and can input various instruction signals.

As shown in FIG. 15, the scanner drive system intermittently moves the optical head 35 in the main scanning direction and the main scanning stepping motor 65, and intermittently moves the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

Further, as shown in FIG. 15, the detection system of the scanner comprises a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser pumping light source 21, the second laser pumping light source 22 or the third laser pumping light source 22 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The radiation data recorded on the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 is read by the scanner configured as described above in the following manner to obtain biochemical analysis data. Is generated.

First, the dichroic mirrors 39a and 39 having the property of transmitting light having a wavelength of 640 nm and reflecting light having a wavelength longer than 640 nm by the user.
a fourth optical head 35 including b, 39c and 39d,
Attached to the endless belt 66 of the scanner, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40.

Next, the user operates the keyboard 7
An instruction signal for scanning the stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 with the laser light 24 is input to the first column.

The instruction signal input to the keyboard 71 is
When the control unit 70 receives the instruction signal and receives the instruction signal, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, moves the filter unit 48, and emits the photostimulable phosphor. Only the light in the wavelength range of photostimulable light 45 is transmitted,
The filter member 51d provided with the filter 52d having the property of cutting the light of the wavelength of 40 nm is located in the optical path of the stimulated emission 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, Of the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10, the first stimulable phosphor layer region 12 is located at a position where the laser light 24 can be irradiated.
When it is determined that the optical head 35 has reached, a drive stop signal is output to the main scanning stepping motor 65, and a drive signal is output to the first laser excitation light source 21 to activate the first laser excitation light source 21, The laser light 24 having a wavelength of 640 nm is emitted.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 a of the perforated mirror 33 and enters the concave mirror 34.

The laser beam 24 incident on the concave mirror 34
Is reflected by the concave mirror 34, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36, and by the aspherical lens 37, the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 placed on the glass plate 41 of the stage 40.
Is focused on.

In this embodiment, the photostimulable phosphor layer regions 12 are formed by embedding the photostimulable phosphor in the through holes 13 formed in the nickel support 11. Therefore, the laser light 24 is scattered in each stimulable phosphor layer region 12 and is incident on the adjacent stimulable phosphor layer regions 12 to be adjacent to each other. It becomes possible to effectively prevent the stimulable phosphor contained therein from being excited.

The laser light 24 causes the stimulable phosphor sheet 10 to emit light.
First stimulable phosphor layer region 1 formed on the support 11 of
When incident on 2, the stimulable phosphor contained in the first stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser light 24 to generate the first stimulable phosphor. The photostimulable light 45 is omnidirectionally emitted from the stimulable phosphor layer region 12.

The photostimulable light 45 emitted from the first photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 and incident on the aspherical lens 37 provided in the fourth optical head 35 is
The light is focused on the mirror 36 by the aspherical lens 37.

On the other hand, it is emitted from the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 toward the side of the aspherical lens 37 of the fourth optical head 35, and is collected. The photostimulable rays 45 incident on the optical lenses 38a, 38b, 38c, and 38d are dichroic mirrors 39a and 39a, respectively.
The light is focused on 39b, 39c, and 39d.

Here, the dichroic mirrors 39a, 39b, 39c, 39 provided on the fourth optical head 35 are provided.
d reflects only the light in the wavelength range of the stimulated emission 45, and 640n
The photostimulable light 45 emitted from the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 has the property of transmitting light having a wavelength of m.
a, 39b, 39c, 39d is reflected, is incident on the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10, and is diffusely reflected. It is incident on the aspherical lens 37, is condensed on the mirror 36 by the aspherical lens 37, is reflected on the same side as the optical path of the laser light 24 by the mirror 36, is made into parallel light, and is incident on the concave mirror 34. .

On the other hand, the photostimulable light 4 which is diffusely reflected by the first photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 and is incident on the condenser lenses 38a, 38b, 38c and 38d.
5 are dichroic mirrors 39a and 39, respectively.
b, 39c, 39d are condensed and similarly sent back to the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10, diffusely reflected, and part of the diffusely reflected photostimulated light 45. Enters the aspherical lens 37.

While the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is being irradiated with the laser beam 24,
The light is emitted from the first stimulable phosphor layer region 12, and the condenser lenses 38a, 38b, 38c on the side of the aspherical lens 37,
Dichroic mirror 3 with stimulus 45 incident on 38d
The reflection by 9a, 39b, 39c, 39d and the diffuse reflection of the stimulable light 45 by the first photostimulable phosphor layer region 12 are repeated, and the aspheric lens 37 of the aspherical lens 37 is emitted from the first photostimulable phosphor layer region 12. A part of the emitted photostimulable light 45 toward the side enters the aspherical lens 37, and is condensed on the mirror 36 by the aspherical lens 37.

On the other hand, together with the stimulus light 45, the light enters the condenser lenses 38a, 38b, 38c and 38d arranged on the side of the aspherical lens 37 of the fourth optical head 35, and the dichroic mirrors 39a, 39b and 39c. , 39d, the laser beam 24 is focused on the dichroic mirrors 39a, 39b, 39c, 3 provided on the fourth optical head 35.
Since 9d has a property of transmitting light having a wavelength of 640 nm, the dichroic mirrors 39a, 39b, 39
c, 39d and therefore the laser light 24 is sent back to the first stimulable phosphor layer region 12 and already
The stimulable phosphor contained in the first stimulable phosphor layer region 12 that has emitted the stimulable light 45 is repeatedly excited to emit the stimulable light 45, and the first stimulable light is emitted. It is possible to reliably prevent the amount of the stimulated emission light 45 emitted from the phosphor layer region 12 from becoming excessive.

Therefore, in this embodiment, from the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 to the aspherical lens 3 provided in the fourth optical head 35.
The emitted photostimulable light 45 toward the side of 7 is returned to the first photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10, and the first photostimulable phosphor layer region 12 is emitted. Is reflected by the aspherical lens 37, is focused on the mirror 36 by the aspherical lens 37, and is emitted from the first stimulable phosphor layer region 12 directly to the aspherical lens 37. At least a part of the stimulus light 45 that has not entered can be condensed on the mirror 36 by the aspherical lens 37, so that the light collection efficiency of the stimulus light 45 can be significantly improved. Become.

The photostimulable light 45 condensed on the mirror 36 is reflected by the mirror 36 to the same side as the optical path of the laser light 24, becomes parallel light, and enters the concave mirror 34.

The photostimulable light 45 incident on the concave mirror 38 is
It is reflected by the concave mirror 38 and enters the perforated mirror 34.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and enters the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off light having a wavelength of m, light having a wavelength of 640 nm, which is excitation light, is cut, and light in the wavelength range of stimulable light 45 emitted from the stimulable phosphor layer region 12 is emitted. Only the light passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog data photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53 and converted into digital data.
It is output to the data processing device 54.

When a predetermined time has elapsed after the first laser excitation light source 21 was turned on, the control unit 7
0 outputs a drive stop signal to the first laser excitation light source 21 to stop the drive of the first laser excitation light source 21, and also outputs a drive signal to the main scanning stepping motor 65 to output the optical head 35. And the stimulable phosphor sheet 10
It is moved by one pitch equal to the distance between adjacent photostimulable phosphor layer regions 12 formed on the support 11.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Are moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 12, and the first laser excitation light source 21
The laser light 24 emitted from the stimulable phosphor sheet 1
When it is determined that the optical head 35 has been moved to a position where the second stimulable phosphor layer region 12 formed on the support 11 of 0 can be irradiated, the control unit 70 causes the first laser excitation light source 21 to move. A driving signal is output, the first laser excitation light source 21 is turned on, and the laser beam 24 causes the second stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 to be irradiated. Excite the stimulable phosphor contained therein.

Similarly, the laser beam 24 is irradiated to the second stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 for a predetermined time, and the second stimulable phosphor layer region 12 is irradiated.
The stimulable phosphor contained in the stimulable phosphor layer region 12 of is excited, and the stimulable light 45 emitted from the second stimulable phosphor layer region 12 is converted by the photomultiplier 50 into When photoelectrically detected and an analog signal is generated, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21 to turn off the first laser excitation light source 21 and perform main scanning. A drive signal is output to the stepping motor 65 to move the optical head 35 by one pitch equal to the distance between adjacent photostimulable phosphor layer regions 12.

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 input from the linear encoder 67, Optical head 35
Was moved by one line in the main scanning direction, and the scanning of the stimulable phosphor layer region 12 of the first line formed on the support 11 of the stimulable phosphor sheet 10 with the laser beam 24 was completed. If the control unit 70 determines that
The drive signal is output to the main scanning stepping motor 65,
The optical head 35 is returned to the original position, and a drive signal is output to the sub-scanning pulse motor 61 to move the movable substrate 63 by one line in the sub-scanning direction.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63
However, when it is determined that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the stimulable phosphor layer region 12 of the first line formed on the support 11 of the stimulable phosphor sheet 10 to be the first line. First, the first laser excitation light source 2
In the same manner as the irradiation of the laser beam 24 emitted from No. 1, the stimulable phosphor layer region 12 of the second line formed on the support 11 of the stimulable phosphor sheet 10 is sequentially irradiated.
Laser light 24 emitted from the first laser excitation light source 21
Is irradiated to excite the stimulable phosphor contained in the stimulable phosphor layer region 12 on the second line, and the luminescence emitted from the stimulable phosphor layer region 12 on the second line. The exhaust 45 is sequentially detected photoelectrically by the photomultiplier 50.

The analog signal photoelectrically detected by the photomultiplier 50 and generated is converted into a digital signal by the A / D converter 53 and sent to the data processor 54.

Thus, all the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are
Laser light 24 emitted from the first laser excitation light source 21
The stimulable phosphor 45 included in the stimulable phosphor layer region 12 is excited by the stimulable phosphor 45 and emitted.
Is photoelectrically detected by the photomultiplier 50, and the generated analog signal is converted into a digital signal by the A / D converter 53, and the data processing device 5
4, the drive stop signal is output from the control unit 70 to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped.

As described above, the stimulable phosphor sheet 1 is used.
The radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the support 11 of No. 0 is read and biochemical analysis data is generated.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is read to generate the biochemical analysis data, first, Depending on the user, the first optical head 35, the second optical head 35, or the third optical head 35 may be an endless scanner depending on the type of fluorescent substance such as a fluorescent dye that labels a substance of biological origin. Attached to the belt 66, the biochemical analysis unit 1
It is set on the transparent glass plate 41 of the stage 40.

Next, the user operates the keyboard 7
1 is recorded in a number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 together with a fluorescent substance identification signal for identifying a fluorescent substance such as a fluorescent dye that labels a substance of biological origin. An instruction signal to read the existing fluorescence data is input.

The fluorescent substance specifying signal and the instruction signal input to the keyboard 71 are input to the control unit 70, and when the control unit 70 receives the fluorescent substance specifying signal and the instruction signal, the control unit 70 stores a memory ( According to a table stored in (not shown), the laser excitation light source to be used is determined, and any one of the filters 52a, 52b and 52c is set to the fluorescence 4
It is determined whether or not it is located in the optical path of No. 5.

For example, Rhodamine (registered trademark), which can be most efficiently excited by a laser having a wavelength of 532 nm, as a fluorescent substance for labeling a substance derived from a living body.
Is used to transmit light with a wavelength of 532 nm and to transmit 532n
An alternative optical head 35 including dichroic mirrors 39a, 39b, 39c, 39d having a property of reflecting light having a wavelength longer than m is attached to an endless belt 66 of the scanner, and by Rhodamine,
When a fluorescent substance specifying signal indicating that the substance of biological origin is labeled is input to the keyboard 71, the control unit 70 selects the second laser excitation light source 22 and also selects the filter 52b. The drive signal is output to the unit motor 72, and the filter unit 4
8 to move light of 532 nm wavelength to cut, 53
A filter member 51b provided with a filter 52b having a property of transmitting light having a wavelength longer than 2 nm is positioned in the optical path of the fluorescence 45 to be emitted from the biochemical analysis unit 1.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder. Of the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, it is determined that the optical head 35 has reached the position where the laser beam 24 can be applied to the first absorptive region 4. Then, a stop signal is output to the main scanning stepping motor 65, and a drive signal is output to the second laser excitation light source 22 to activate the second laser excitation light source 22 and emit the laser light 24 having a wavelength of 532 nm. Let

The laser light 24 emitted from the second laser excitation light source 22 is made into parallel light by the collimator lens 30, and then enters the first dichroic mirror 27 and is reflected.

The laser beam 24 reflected by the first dichroic mirror 27 passes through the second dichroic mirror 28 and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by mirror 32
4 passes through the hole 33 a of the perforated mirror 33 and enters the concave mirror 34.

Laser light 24 incident on the concave mirror 34
Is reflected by the concave mirror 34, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 26 and focused by the aspherical lens 37 on the biochemical analysis unit 1 mounted on the glass plate 41 of the stage 40.

In the present embodiment, a large number of absorptive regions 4 of the biochemical analysis unit 1 are formed on a substrate 2 made of stainless steel having a property of attenuating light, separated from each other. From the above, the laser light 24 that has entered each of the absorptive regions 4 of the biochemical analysis unit 1 is scattered within the substrate 2 of the biochemical analysis unit 1 and enters the adjacent absorptive regions 4 to be adjacent to each other. Further, it becomes possible to effectively prevent the fluorescent substance contained in the adsorptive region 4 from being excited.

The laser beam 24 is emitted from the biochemical analysis unit 1
When incident on the first absorptive region 4 formed on the substrate 2 of the above, the laser light 24 causes a fluorescent substance such as a fluorescent dye contained in the first absorptive region 4 of the biochemical analysis unit 1, for example, Rhodamine is excited and fluorescence 45
It is emitted omnidirectionally.

Fluorescence 45 emitted from rhodamine contained in the first absorptive region 4 of the biochemical analysis unit 1 and incident on the aspherical lens 37 provided in the second optical head 35 is the aspherical lens. The light is focused on the mirror 36 by 37.

On the other hand, a condenser lens 38a which is emitted from the absorptive region 4 of the biochemical analysis unit 1 and is arranged beside the aspherical lens 37 of the second optical head 35,
The fluorescence 45 incident on 38b, 38c and 38d is dichroic mirrors 39a, 39b, 39c and 3 respectively.
It is focused on 9d.

Here, the dichroic mirrors 39a, 39b, 39c, 39d of the second optical head 35 are 53
The fluorescence 45 emitted from the first adsorptive region 4 of the biochemical analysis unit 1 has the property of transmitting light having a wavelength of 2 nm and reflecting light having a wavelength longer than 532 nm.
Is a dichroic mirror 39a, 39b, 39c, 3
A part of the fluorescence 45 reflected by 9d and incident on the first absorptive region 4 of the biochemical analysis unit 1 and diffused and diffused is incident on the aspherical lens 37 and the aspherical lens 37. Is focused on the mirror 36, reflected by the mirror 36 on the same side as the optical path of the laser light 24, and made into parallel light, which then enters the concave mirror 34.

On the other hand, the second optical head 3 is irregularly reflected by the first absorptive region 4 of the biochemical analysis unit 1.
5, condenser lenses 38a, 38b, 38c, 3
The fluorescence 45 that has entered 8d is condensed on the dichroic mirrors 39a, 39b, 39c, and 39d, respectively, and is similarly sent back to the first absorptive region 4 of the biochemical analysis unit 1 and diffusely reflected, Diffuse reflected fluorescence 45
Part of the light enters the aspherical lens 37.

While the first absorptive region 4 of the biochemical analysis unit 1 is being irradiated with the laser beam 24, it is emitted from the first absorptive region 4 and condensed on the side of the aspherical lens 37. Dichroic mirrors 39a, 39b, 39c of the fluorescence 45 that have entered the lenses 38a, 38b, 38c, 38d,
Reflection by 39d, fluorescence by the first absorptive region 4 45
The diffuse reflection is repeated, and a part of the fluorescence 45 emitted from the first absorptive region 4 toward the side of the aspherical lens 37 enters the aspherical lens 37, and by the aspherical lens 37, It is focused on the mirror 36.

On the other hand, together with the fluorescent light 45, the light enters the condenser lenses 38a, 38b, 38c, 38d arranged on the side of the aspherical lens 37 of the second optical head 35, and the dichroic mirrors 39a, 39b, 39c, 39d. The laser light 24 focused on the dichroic mirrors 39 a, 39 b, 39 c, 39 provided on the second optical head 35 is
Since d has a property of transmitting light having a wavelength of 532 nm, the dichroic mirrors 39a, 39b, 39
Therefore, the laser light 24 is transmitted back to the adsorptive region 4 containing the excited fluorescent substance, and the fluorescent substance that has already emitted the fluorescent substance 45 is repeatedly excited to emit the fluorescent substance 45. Thus, it is possible to reliably prevent the amount of the fluorescent light 45 emitted from the adsorptive region 4 from becoming excessive.

Therefore, in this embodiment, the light is emitted from the first absorptive region 4 of the biochemical analysis unit 1 toward the side of the aspherical lens 37 provided in the second optical head 35. The stimulated emission 45 is returned to the first absorptive region 4 of the biochemical analysis unit 1, is diffusely reflected by the first absorptive region 4, and is incident on the aspherical lens 37,
When the light is condensed on the mirror 36 by the aspherical lens 37 and is emitted from the first absorptive region 4, the aspherical lens 3
At least a part of the fluorescence 45 that did not directly enter 7
Since the aspherical lens 37 allows light to be collected on the mirror 36, it is possible to significantly improve the light collecting efficiency of the fluorescent light 45.

The fluorescent light 45 collected on the mirror 36 is reflected by the mirror 36 to the same side as the optical path of the laser light 24, becomes parallel light, and enters the concave mirror 34.

The fluorescent light 45 which has entered the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.

The fluorescent light 45 that has entered the perforated mirror 34 is
As shown in FIG. 7, the light is reflected downward by the perforated mirror 34 formed by the concave mirror and enters the filter 52b of the filter unit 48.

Since the filter 52b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm, which is excitation light, is cut and emitted from rhodamine. Fluorescence 45
Only the light in the wavelength range of 1 passes through the filter 52b and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected by the photomultiplier 50 and generated is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

When a predetermined time elapses after the second laser excitation light source 22 is turned on, the control unit 7
0 outputs a drive stop signal to the second laser excitation light source 22 to stop the drive of the second laser excitation light source 22, and also outputs a drive signal to the main scanning stepping motor 65 to output the optical head 35. The biochemical analysis unit 1
It is moved by one pitch equal to the distance between the adsorbing regions 4 formed adjacent to each other.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adjacent absorptive regions 4 formed in the biochemical analysis unit 1,
Laser light 24 emitted from the second laser excitation light source 22
When it is determined that the optical head 34 has been moved to a position where the second absorptive region 4 formed in the biochemical analysis unit 1 can be irradiated, the control unit 70 drives the second laser excitation light source 22. A signal is output, the second laser excitation light source 22 is turned on, and the laser light 24 causes
The fluorescent substance contained in the second absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1, for example, rhodamine is excited.

Similarly, the laser beam 24 is applied to the second absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 for a predetermined time, and the second absorptive region 4 is irradiated.
The fluorescence 45 emitted from the photomultiplier 50
When it is photoelectrically detected and analog data is generated, the control unit 70 outputs a drive stop signal to the second laser excitation light source 22 to turn off the second laser excitation light source 22, and A drive signal is output to the main scanning stepping motor 65 to output the optical head 35.
Are moved by one pitch equal to the distance between the adsorbing regions 4 formed on the substrate 2 of the biochemical analysis unit 1.

In this way, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 input from the linear encoder 67, Optical head 35
However, if it is determined that all the absorptive regions 4 of the first line formed on the substrate 2 of the biochemical analysis unit 1 have been moved by one line in the main scanning direction and scanned by the laser light 24, The control unit 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position, and outputs a drive signal to the sub scanning pulse motor 61 to move the movable substrate 6
3 is moved by one line in the sub-scanning direction.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63
However, if it is determined that the line has been moved by one line in the sub-scanning direction, the control unit 70 sequentially moves to the absorptive region 4 of the first line formed on the substrate 2 of the biochemical analysis unit 1 in order. Rhodamine contained in the absorptive region 4 of the second line formed on the substrate 2 of the biochemical analysis unit 1 in exactly the same manner as when the laser beam 24 emitted from the laser excitation light source 22 of No. 2 was irradiated. Excites the second
The fluorescence 45 emitted from the absorptive region 4 of the line is sequentially detected photoelectrically by the photomultiplier 50.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processing device 54.

In this way, all the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned by the laser beam 24 emitted from the second laser excitation light source 22, and the biochemical analysis unit is scanned. Rhodamine contained in the adsorptive region 4 formed on the substrate 2 of No. 1 is excited,
The emitted fluorescence 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is
When converted to digital data by the A / D converter 53 and sent to the data processing device 54, a drive stop signal is output from the control unit 70 to the second laser excitation light source 22 and the second laser excitation light source 22 is excited. The driving of the light source 22 is stopped.

As described above, the fluorescence data of the fluorescent substance recorded in the numerous absorptive regions 4 formed in the biochemical analysis unit 1 is read and the biochemical analysis digital data is generated.

In this embodiment, when the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is read, the mirror 36 and the Equipped with a spherical lens 37,
On the side of the aspherical lens 37, four condenser lenses 38a,
38b, 38c, 38d, and four dichroic mirrors 39a, 39b having a property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength range of the photostimulable light 45.
A fourth optical head 35 including 39c and 39d is attached to an endless belt 66 of the scanner, and a large number of photostimulable phosphor layers formed on the support 11 of the stimulable phosphor sheet 10 by the laser light 24. Since the region 12 is scanned, the stimulable phosphor sheet 10 is irradiated with the laser light 24.
The stimulable phosphor contained in each stimulable phosphor layer region 12 of is excited and omnidirectionally emitted from each stimulable phosphor layer region 12 of the stimulable phosphor sheet 10. The stimulated light 45 incident on the aspherical lens 37 of the optical head 35 of No. 4 is condensed on the mirror 36 by the aspherical lens 37.

On the other hand, condensing lenses 38a, 38b, 3 which are emitted from the stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 and are arranged on the side of the aspherical lens 37.
The photostimulable light 45 that has entered the light beams 8c and 38d is condensed on the dichroic mirrors 39a, 39b, 39c, and 39d, respectively, and the dichroic mirrors 39a, 39b, 39c, and 39d of the fourth optical head 35 reflect the photostimulable light 45. Of the stimulable phosphor sheet 10 having the property of reflecting only the light in the wavelength region of, and being reflected by the dichroic mirrors 39a, 39b, 39c, 39d, and stimulating light 45 thereof being emitted. A part of the photostimulable light 45 which is incident on the phosphor layer region 12 and diffused and reflected is incident on the aspherical lens 37 and is reflected by the aspherical lens 37.
The light is focused on the beam No. 6 and is reflected by the mirror 36 on the same side as the optical path of the laser light 24 to be parallel light, which then enters the concave mirror 34.

On the other hand, the photostimulable light 45, which is diffusely reflected by the photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 and again enters the condenser lenses 38a, 38b, 38c, and 38d, is dichroic. Mirrors 39a, 39
b, 39c, 39d, and similarly, the stimulable light 45 is sent back to the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 from which it has been emitted, and is diffusely reflected and diffusely reflected. Part of the exhaust light 45 enters the aspherical lens 37.

As described above, each stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is emitted from the stimulable phosphor layer region 12 while being irradiated with the laser beam 24, and is aspherical. Condensing lenses 38a, 38b, 3 on the side of the lens 37
Reflection of the photostimulable light 45 incident on 8c, 38d by the dichroic mirrors 39a, 39b, 39c, 39d and diffuse reflection of the photostimulable light 45 by the photostimulable phosphor layer region 12 from which the photostimulable light 45 is emitted are repeated. A part of the stimulable light 45 emitted from the stimulable phosphor layer region 12 toward the side of the aspherical lens 37 is incident on the aspherical lens 37, and the aspherical lens 37 causes the mirror 36 to move. Since the light is focused on the photostimulable phosphor layer region 12 by the laser light 24,
At least a part of the stimulable light 45 that did not directly enter the aspherical lens 37 when the stimulable phosphor contained in was excited and emitted from each stimulable phosphor layer region 12, The aspherical lens 37 makes it possible to focus light on the mirror 36. Therefore, according to the present embodiment, the four condensing lenses 38a, 3a are simply provided on the side of the aspherical lens 37.
4 dichroic mirrors 39a, 39b, 3 having the property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength region of the photostimulable light 45.
Only by using the fourth optical head 35 equipped with 9c and 39d, the stimulating stimulus 45 emitted non-directionally can be efficiently
It becomes possible to collect light and generate data for biochemical analysis with high sensitivity.

Further, in this embodiment, a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are used.
When reading the fluorescence data recorded in, the mirror 36 and the aspherical lens 37 are provided according to the type of the fluorescent substance labeling the substance of biological origin, and further on the side of the aspherical lens 37. Four condenser lenses 38a, 38b,
38c, 38d and light with a wavelength of 640 nm are transmitted,
Four dichroic mirrors 39a, 39b, 39 having a property of reflecting light having a wavelength longer than 40 nm
The first optical head 35 provided with c and 39d, and the four condensing lenses 38a, 38b, and 3 on the side of the aspherical lens 37.
8c, 38d and light with a wavelength of 532 nm are transmitted,
Four dichroic mirrors 39a, 39b, 39c, which have the property of reflecting light having a wavelength longer than 2 nm,
On the side of the second optical head 35 or the aspherical lens 37 provided with 39d, four condenser lenses 38a, 38b,
38c, 38d and light having a wavelength of 473 nm are transmitted,
Four dichroic mirrors 39a, 39b, 39 having a property of reflecting light having a wavelength longer than 73 nm
A third optical head 35 equipped with c and 39d is attached to the endless belt 66 of the scanner, and the laser beam 24
Since a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned by the laser beam 24,
The fluorescent substance contained in each absorptive region 4 of the biochemical analysis unit 1 is excited by this, and is emitted omnidirectionally from each absorptive region 4 of the biochemical analysis unit 1 to form an aspheric lens. The fluorescence 45 that has entered the 37 is aspherical lens 37.
Is focused on the mirror 36.

On the other hand, condensing lenses 38a, 38 emitted from each absorptive region 4 of the biochemical analysis unit 1 and arranged on the side of the aspherical lens 37 of the optical head 35.
Fluorescent light 45 that has entered b, 38c, and 38d,
Dichroic mirrors 39a, 39b, 39c, 39d
On the dichroic mirror 3 of the optical head 35.
9a, 39b, 39c and 39d transmit the light of the wavelength of the laser light 24 used for exciting the fluorescent material, and the laser light 2
Since it has a property of reflecting light having a wavelength longer than 4, the fluorescence 45 emitted from the first absorptive region 4 of the biochemical analysis unit 1 is dichroic mirror 39a,
The fluorescence 45 reflected by 39b, 39c, 39d is incident on the absorptive region 4 of the biochemical analysis unit 1 from which the fluorescence 45 is emitted, and a part of the fluorescence 45 diffused and reflected irregularly is aspherical lens 37. To the mirror 36 by the aspherical lens 37, and by the mirror 36,
The laser light 24 is reflected to the same side as the optical path, becomes parallel light, and enters the concave mirror 34.

On the other hand, the fluorescence 45 which is diffusely reflected by the respective absorptive regions 4 of the biochemical analysis unit 1 and is incident on the condenser lenses 38a, 38b, 38c and 38d provided on the optical head 35 is respectively dichroic mirror. 39
a, 39b, 39c, 39d are focused and similarly,
The fluorescence 45 is sent back to the absorptive region 4 of the biochemical analysis unit 1 from which it has been emitted, and is diffusely reflected, and a part of the diffusely reflected fluorescence 45 enters the aspherical lens 37.

As described above, while the absorptive regions 4 of the biochemical analysis unit 1 are being irradiated with the laser light 24, the absorptive regions 4 are emitted from the absorptive regions 4 and condensed on the side of the aspherical lens 37. Dichroic mirrors 39a, 39b, 39 for the fluorescence 45 incident on the lenses 38a, 38b, 38c, 38d.
The reflection of c and 39d and the diffuse reflection of the fluorescence 45 by the absorptive area 4 from which the fluorescence 45 is emitted are repeated, and the fluorescence 45 emitted from each absorptive area 4 toward the side of the aspherical lens 37. Part of the light enters the aspherical lens 37,
Since the aspherical lens 37 collects the light on the mirror 36, the laser light 24 excites the fluorescent substance contained in each absorptive region 4 to emit an aspherical surface when emitted from each absorptive region 4. Fluorescence 45 that did not directly enter the lens 37
It is possible to focus at least a part of the light on the mirror 36 by the aspherical lens 37. Therefore, according to the present embodiment, it is possible to simply change the type of the fluorescent substance labeling the substance of biological origin. On the side of the aspherical lens 37,
Four condenser lenses 38a, 38b, 38c, 38d,
Only the optical head 35 provided with the four dichroic mirrors 39a, 39b, 39c, 39d having the property of transmitting the light of the wavelength of the laser light 24 and reflecting the light of the longer wavelength than the laser light 24 is used. Then, it becomes possible to efficiently and efficiently collect the stimulated stimulus light 45 emitted omnidirectionally and to generate biochemical analysis data with high sensitivity.

Further, according to this embodiment, the photostimulable light 45
Alternatively, together with the fluorescent light 45, the condenser lenses 38a, 38 arranged beside the aspherical lens 37 of the optical head 35.
b, 38c, 38d are incident on the dichroic mirror 3
Laser light 2 focused on 9a, 39b, 39c, 39d
The reference numeral 4 indicates that the dichroic mirrors 39a, 39b, 39c and 39d provided in the optical head 35 have a property of transmitting the light of the wavelength of the laser light 24, and thus the dichroic mirrors 39a, 39b, 39c and 39d are transmitted. Then
Therefore, the laser light 24 is sent back to the stimulable phosphor layer region 12 containing the excited stimulable phosphor, and already,
The photostimulable phosphor that has emitted the photostimulable light 45 is repeatedly excited to emit the photostimulable light 45, and the amount of the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 becomes excessive. It is possible to reliably prevent such a change, and the laser beam 24 is sent back to the absorptive region 4 containing the excited fluorescent substance to repeatedly excite the fluorescent substance that has already emitted the fluorescence 45. Thus, it becomes possible to surely prevent the fluorescence 45 from being emitted and the light amount of the fluorescence 45 emitted from the adsorptive region 4 to be excessive.

FIG. 16 is a schematic perspective view showing another example of the stimulable phosphor sheet.

As shown in FIG. 16, the stimulable phosphor sheet 80 comprises a support 81 and a stimulable phosphor layer 82 uniformly formed on one surface of the support 81. .

FIG. 17 shows that the stimulable phosphor sheet 80 is produced by the radioactive labeling substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1.
FIG. 9 is a schematic cross-sectional view showing a method of exposing the stimulable phosphor contained in the stimulable phosphor layer 82 formed on the surface of the support 81 of FIG.

As shown in FIG. 17, the radioactive labeling substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 causes the stimulable phosphor sheet 80 to be stored. When the stimulable phosphor contained in the stimulable phosphor layer 82 formed on the surface of the support 81 is exposed, the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 is biochemical. Many absorptive regions 4 of the analysis unit 1
The stimulable phosphor sheet 80 is superposed on the biochemical analysis unit 1 so as to be opposed to, and is held for a predetermined time.

As a result, the electron beam (β-ray) emitted from the radiolabeled substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 causes accumulation. The stimulable phosphor contained in the stimulable phosphor layer 82 formed on the support 81 of the phosphor sheet 80 is exposed.

At this time, since the large number of absorptive regions 4 of the biochemical analysis unit 1 are formed separately from each other on the substrate 2 formed of stainless steel having a property of attenuating radiation, Even if the electron beam (β-ray) emitted from each adsorptive region 4 of the chemical analysis unit 1 has a high energy, the electron beam (β-ray) is generated within the substrate 2 of the biochemical analysis unit 1. It is possible to effectively prevent the scattering, and therefore, the adsorption is performed by the electron beam (β-ray) emitted from the radiolabel substance contained in the adsorptive region 4 of the biochemical analysis unit 1. It becomes possible to selectively expose only the stimulable phosphor contained in the region of the stimulable phosphor layer 82 facing the photoactive region 4.

[0285] FIG. 18 shows that when the electron beam (β ray) emitted from the radiolabeled substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, FIG. 9 is a schematic perspective view of the stimulable phosphor sheet 80 after exposure of the stimulable phosphor contained in the stimulable phosphor layer 82, showing a large number of stimulable phosphor sheets formed on the substrate 2 of the biochemical analysis unit 1. The stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 is irradiated with the electron beam (β-ray) emitted from the radioactive labeling substance selectively contained in the absorptive region 4. As a result of exposing the stimulable phosphor contained therein, a large number of exposed regions 85 are formed in the stimulable phosphor layer 82 of the stimulable phosphor sheet 80.

Thus, the radiation data of the radioactive labeling substance is transferred to the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80.

FIG. 19 is a schematic bottom view of an optical head provided in a scanner provided in a scanner according to another preferred embodiment of the present invention, and FIG. 20 is a schematic end face taken along line EE thereof. It is a figure.

As shown in FIGS. 19 and 20, the optical head 90 provided in the scanner according to this embodiment.
Is provided with a mirror 91 and an aspherical lens 92, similarly to the optical head 35 shown in FIGS.
It is provided with a spherical dichroic mirror 93 in which a portion corresponding to the aspherical lens 92 is cut out.

In this embodiment, four types of optical heads 90 equipped with spherical dichroic mirrors 93 having different characteristics are prepared, and a predetermined optical head 90 is a scanner depending on the labeling substance and the laser excitation light source used. It is configured to be attached to the endless belt 66.

The first optical head 90 uses the first laser excitation light source 21, and uses the substrate 2 of the biochemical analysis unit 1.
The spherical dichroic mirror 93 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Has a property of transmitting light having a wavelength of 640 nm and reflecting light having a wavelength longer than 640 nm.

The second optical head 90 uses the second laser excitation light source 22 and uses the substrate 2 of the biochemical analysis unit 1.
The spherical dichroic mirror 93 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Has a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm.

The third optical head 90 uses the third laser excitation light source 23 and uses the substrate 2 of the biochemical analysis unit 1.
The spherical dichroic mirror 93 is used when exciting a fluorescent substance such as a fluorescent dye contained in a large number of the absorptive regions 4 formed in the above to read the fluorescence 45.
Has a property of transmitting light having a wavelength of 473 nm and reflecting light having a wavelength longer than 473 nm.

The fourth optical head 90 uses the first laser excitation light source 21 to emit the luminescent material contained in the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80. The spherical dichroic mirror 93 is used when the photostimulable phosphor is excited to read the photostimulable light 45 emitted from the photostimulable phosphor layer 82. It has the property of reflecting only the light and transmitting the light of the wavelength of 640 nm.

FIG. 21 is a block diagram showing a control system, an input system, a drive system and a detection system of a scanner according to another preferred embodiment of the present invention.

As shown in FIG. 21, the scanner is provided with an optical head 90 in place of the optical head 35 of the scanner according to the above-described embodiment, and is replaced with the main scanning stepping motor 65 by using the optical head 90 in the main scanning. The scanner has the same configuration as the scanner according to the above-described embodiment except that a main scanning motor 95 that continuously moves in the direction is provided.

The scanner according to the present embodiment configured as described above is recorded on the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 in the following manner. The radiation data that is present is read and biochemical analysis data is generated.

First, the fourth optical head 35 having the spherical dichroic mirror 93, which has the property of transmitting light having a wavelength of 640 nm and reflecting light having a wavelength longer than 640 nm, is set by the user to the endless belt 66 of the scanner. And the stimulable phosphor sheet 80 is mounted on the glass plate 41 of the stage 40.

Next, the user operates the keyboard 7
An instruction signal to the effect that the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 is scanned by the laser light 24 is input to 1.

The instruction signal input to the keyboard 71 is
It is output to the control unit 70.

When an instruction signal is received from the keyboard 71,
The control unit 70 follows the instruction signal
A drive signal is output to the filter unit motor 72, the filter unit 48 is moved, and only light in the wavelength range of the photostimulable light 45 emitted from the photostimulable phosphor is transmitted, and 640 nm
The filter member 51d provided with the filter 52d having the property of cutting off the light of the wavelength is placed in the optical path of the stimulated emission 45.

Next, the control unit 70 outputs a drive signal to the first laser excitation light source 21, activates the first laser excitation light source 21, and causes the laser light 24 having a wavelength of 640 nm to be emitted.

The laser light 24 emitted from the first laser excitation light source 21 is collimated by the collimator lens 25, and then enters the mirror 26 to be reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 a of the perforated mirror 33 and enters the concave mirror 34.

Laser light 24 incident on the concave mirror 34
Is reflected by the concave mirror 34 and enters the fourth optical head 90.

The laser light 24 incident on the fourth optical head 90 is reflected by the mirror 91, and the aspherical lens 9
2, the light is condensed at a position corresponding to the center of the spherical dichroic mirror 93 in the exposure area 85 of the stimulable phosphor layer 82 formed on the stimulable phosphor sheet 80 placed on the glass plate 41 of the stage 40. To be done.

As a result, the stimulable phosphor contained in the exposed region 85 of the stimulable phosphor layer 82 is excited by the laser beam 24, and is exposed from the exposed region 85 of the stimulable phosphor layer 82. The stimulated emission 45 is omnidirectionally emitted.

Photostimulable phosphor 8 of stimulable phosphor sheet 80
The second optical head 90 emits light from the second exposure area 85.
45 which is incident on the aspherical lens 92 provided on the
Is condensed on the mirror 91 by the aspherical lens 92.

On the other hand, the luminescence emitted from the exposed region 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 toward the side of the aspherical lens 37 of the fourth optical head 35. The exhaust 45 is incident on the spherical dichroic mirror 93.

In this embodiment, the spherical dichroic mirror 93 provided in the fourth optical head 90 reflects only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and has a wavelength of 640 nm. Since it has a property of transmitting light of a wavelength, the stimulable light 45 emitted from the exposed area 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 and incident on the spherical dichroic mirror 93 is a spherical surface. The dichroic mirror 93 reflects the photostimulable light 45 toward a position corresponding to the center of the spherical dichroic mirror 93 in the exposure region 85 of the photostimulable phosphor layer 82 from which the photostimulable phosphor layer 82 is emitted.

The photostimulable phosphor layer 8 which is reflected by the spherical dichroic mirror 93 and emits the photostimulable light 45.
The photostimulable light 45 that has entered the second exposure region 85 is diffusely reflected by the exposure region 85 of the photostimulable phosphor layer 82, and a part of the photostimulable light 45 that is diffusely reflected is incident on the aspherical lens 92. The light is focused on the mirror 91 by the aspherical lens 92.

On the other hand, the exposed area 85 of the stimulable phosphor layer 82.
Most of the stimulus light 45 that is diffusely reflected by the spherical surface and does not enter the aspherical lens 92 is generated by the spherical dichroic mirror 9.
It is incident on 3.

The photostimulable light 45 that has entered the spherical dichroic mirror 93 is reflected by the spherical dichroic mirror 93 and enters the exposed region 85 of the photostimulable phosphor layer 82 from which the photostimulable light 45 has been emitted, and the photostimulable light 45 is emitted. A part of the stimulable light 45 diffusely reflected by the exposed region 85 of the exhaustive phosphor layer 82 is incident on the aspherical lens 92, and is condensed on the mirror 91 by the aspherical lens 92.

On the other hand, the laser light 24 incident on the spherical dichroic mirror 93 is emitted from the fourth optical head 90.
Since the spherical dichroic mirror 93 provided in has a property of reflecting only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor and transmitting the light of the wavelength of 640 nm, The laser light 24 that has passed through the dichroic mirror 93 and traveled, and thus excited the stimulable phosphor contained in the exposed region 85 of the stimulable phosphor layer 82,
Again, it becomes possible to reliably prevent the stimulable phosphor contained in the exposed region 85 of the stimulable phosphor layer 82 from being excited.

Aspherical lens 92 of fourth optical head 90
The stimulated light 45 condensed on the mirror 91 is reflected by the mirror 46 on the same side as the optical path of the laser light 34, becomes parallel light, and enters the concave mirror 34.

The photostimulable light 45 incident on the concave mirror 34 is
It is reflected by the concave mirror 34 and enters the perforated mirror 33.

Photostimulation 45 incident on the perforated mirror 33
Is reflected downward by the perforated mirror 33 formed by the concave mirror, and enters the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, only the light in the wavelength range of the photostimulable light 45 emitted from the photostimulable phosphor layer 82 is cut off the light of the wavelength of 640 nm which is the excitation light. Passes through the filter 52d, and the photomultiplier 5
0 is detected photoelectrically.

The analog data photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53 and converted into digital data.
It is output to the data processing device 54.

Based on the position detection signal of the optical head 90 input from the linear encoder 67, the optical head 90
Is moved by one line in the main scanning direction, and it is determined that all the exposure regions 85 of the stimulable phosphor layer 82 located in the first scanning line are scanned by the laser light 24, the control unit. 70 is a main scanning motor 9
5, a drive signal is output to return the optical head 90 to the original position, and a drive signal is output to the sub-scanning pulse motor 61 to move the movable substrate 63 in the sub-scanning direction.
Move only one line.

Based on the position detection signal of the optical head 90 inputted from the linear encoder 67, the optical head 90
Is returned to its original position, and the movable substrate 63
However, if it is determined that the line has been moved by one line in the sub-scanning direction, the control unit 70 similarly similarly locates the stimulable phosphor layer 82 located within the second scan line of the stimulable phosphor sheet 80. The laser light 24 emitted from the first laser excitation light source 21 is radiated to the exposure area 85 of the photo-excited phosphor layer 82, and the luminescence contained in the exposure area 85 of the stimulable phosphor layer 82 located in the second scan line is irradiated. The photomultiplier 50 photoelectrically detects the photostimulable light 45 emitted from the photostimulable phosphor layer 82 by exciting the photostimulable phosphor.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

In this way, the entire surface of the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 is scanned by the laser light 24 emitted from the first laser excitation light source 21 to emit the bright light. The photostimulable phosphor 45 contained in the exposed region 85 of the photostimulable phosphor layer 82 is excited, and the emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50 to generate analog data. Is converted into digital data by the A / D converter 53 and sent to the data processing device 54, the control unit 70
Then, the drive stop signal is output to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped.

The stimulable phosphor sheet 8 is thus obtained.
The radiation data of the radiolabeled substance recorded in the stimulable phosphor layer 82 formed on the support 81 of No. 0 is read, and biochemical analysis data is generated.

When reading fluorescence data recorded in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, depending on the type of fluorescent substance labeling the substance derived from the living body, , The first optical head 90, the second optical head 90, or the third optical head 90 is attached to the endless belt 66 of the scanner, and is accumulated by the laser light 24 emitted from the first laser excitation light source 21. Exactly the same as scanning the exposure area 85 of the stimulable phosphor layer 82 formed on the phosphor sheet 80, the first laser excitation light source 21, the second laser excitation light source 22 or the third laser excitation light source 21 is scanned. Laser light 24 emitted from the light source 23
Scans a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and the fluorescence 45 emitted from the absorptive regions 4 is photoelectrically detected by the photomultiplier 50 and Data for chemical analysis is generated.

In this embodiment, the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80.
When reading the radiation data recorded in, a mirror 91 and an aspherical lens 92 are provided, and light having a wavelength of 640 nm is transmitted to the side of the aspherical lens 92 and the wavelength range of the stimulable light 45. A fourth optical head 90 having a spherical dichroic mirror 93 having a property of reflecting only the light of is attached to the endless belt 66 of the scanner, and the stimulable phosphor sheet 80 is irradiated by the laser light 24.
Since the stimulable phosphor layer 82 formed on the support 81 is scanned, the laser beam 24 included in each of the six regions 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80. When the stimulable phosphor is excited, it is omnidirectionally emitted from each exposed region 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80, and is emitted to the aspherical lens 92 of the fourth optical head 90. The incident stimulated light 45 is condensed on the mirror 91 by the aspherical lens 92.

On the other hand, the luminescence emitted from each exposure region 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 and incident on the spherical dichroic mirror 93 arranged on the side of the aspherical lens 92. Since the spherical dichroic mirror 93 has a property of reflecting only the light in the wavelength region of the photostimulable light 45, the exhaust light 45 has the property of reflecting the spherical dichroic mirror 9.
The photostimulable light 45 reflected by 3 is incident on each exposed region 85 of the photostimulable phosphor layer 82 of the stimulable phosphor sheet 80 of the stimulable phosphor sheet 80, which is diffused and diffused.
A part of 5 is incident on the aspherical lens 92, is condensed on the mirror 91 by the aspherical lens 92, is reflected on the same side as the optical path of the laser beam 24 by the mirror 91, and is made into parallel light, It is incident on the concave mirror 34.

On the other hand, most of the stimulable light 45 which is diffusely reflected by the exposure area 85 of the stimulable phosphor layer area 82 of the stimulable phosphor sheet 80 and does not enter the aspherical lens 92 is a spherical dichroic mirror. Similarly, the photostimulable light 45 is sent back to the exposed region 85 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 from which the photostimulable light 45 has been emitted, and is diffusely reflected and diffusely reflected. A part of 45 is incident on the aspherical lens 37.

Therefore, according to this embodiment, the stimulable phosphor contained in each exposed region 85 of the stimulable phosphor layer 82 formed on the stimulable phosphor sheet 80 is excited by the laser light 24. Then, most of the stimulable light 45 that did not directly enter the aspherical lens 92 when it was emitted from each exposed region 85 of the stimulable phosphor layer 82 is converted by the spherical dichroic mirror 93. Since 45 can be returned to the exposed region 85 where it is emitted, it can be diffused and reflected by the exposed region 85 of the stimulable phosphor layer 82, and can be condensed on the mirror 91 by the aspherical lens 92. Light having a wavelength of 640 nm is transmitted to the side of the spherical lens 92,
Only by using the fourth optical head 90 provided with the spherical dichroic mirror 93 having the property of reflecting only the light in the wavelength region of the stimulated emission 45, the stimulated emission 45 emitted omnidirectionally is It is possible to generate data for biochemical analysis with high efficiency, light collection, and high sensitivity.

Further, according to this embodiment, the spherical dichroic mirror 93 transmits light having a wavelength of 640 nm,
Since it has a property of reflecting only the light in the wavelength region of the stimulated emission 45, the spherical dichroic mirror 93 arranged beside the aspherical lens 92 of the fourth optical head 90 together with the stimulated emission 45. The incident laser light 24 passes through the spherical dichroic mirror 93 and proceeds, and therefore the laser light 24 that excites the stimulable phosphor contained in the exposed region 85 of the stimulable phosphor layer 82 is again emitted. The photostimulable phosphor that has surely prevented excitation of the photostimulable phosphor contained in the exposed region 85 of the photostimulable phosphor layer 82 and has already emitted the photostimulable light 45 is a laser beam. Repeated by 24,
It is exposed and emits photostimulable light 45.
It is possible to reliably prevent generation of noise due to the detection of the above in the generated biochemical analysis data.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the embodiment shown in FIGS. 1 to 15, the support 1 of the stimulable phosphor sheet 10 is used.
When reading the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the mirror 1, the mirror 36
And an aspherical lens 37, and further the aspherical lens 3
On the side of 7, four condenser lenses 38a, 38b, 38
c, 38d, and four dichroic mirrors 39a, 39b, 39c, 39 having a property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength range of the photostimulable light 45.
The fourth optical head 35 provided with d is attached to the endless belt 66 of the scanner, and a large number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 by the laser light 24. Is being scanned, a mirror 36 and an aspherical lens 37 are provided, and four condensing lenses 38a, 38 are provided on the side of the aspherical lens 37.
b, 38c, 38d, and four dichroic mirrors 39a, 39b, 3 having the property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength range of the photostimulable light 45.
Instead of the fourth optical head 35 provided with 9c and 39d,
The mirror 91 and the aspherical lens 92 are provided, and the light having a wavelength of 640 nm is transmitted to the side of the aspherical lens 92 and only the light in the wavelength region of the stimulated emission 45 is reflected. The fourth optical head 90 having the spherical dichroic mirror 93 is attached to the endless belt 66 of the scanner, and a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are scanned. Then, the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 can be read to generate biochemical analysis data.

Further, in the embodiment shown in FIGS. 1 to 15, when reading fluorescence data recorded in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, A mirror 36 and an aspherical lens 37 are provided according to the type of fluorescent substance labeling the substance derived from the living body.
And four condenser lenses 38a, 38b, 38c, 38d and 640n on the side of the aspherical lens 37.
A first optical head 35 provided with four dichroic mirrors 39a, 39b, 39c, 39d having a property of transmitting light having a wavelength of m and reflecting light having a wavelength longer than 640 nm, an aspherical lens. To the side of 37, four condensing lenses 38a, 38b, 38c and 38d and four dichroic having a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm. Mirror 3
Four condensing lenses 38a, 38b, 38c, 38d and 473 nm are provided on the side of the second optical head 35 or the aspherical lens 37 having 9a, 39b, 39c, 39d.
The third optical head 35 including the four dichroic mirrors 39a, 39b, 39c, 39d, which has a property of transmitting light having a wavelength of 4 nm and reflecting light having a wavelength longer than 473 nm, is an endless scanner. A large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned by the laser beam 24 attached to the belt 66, and the type of the fluorescent substance that labels the substance of biological origin According to, a mirror 91 and an aspherical lens 92 are provided,
Further, a first optical head 90 provided with a spherical dichroic mirror 93 having a property of transmitting light having a wavelength of 640 nm and reflecting light having a wavelength longer than 640 nm to the side of the aspherical lens 92, A mirror 91 and an aspherical lens 92 are provided.
A second optical head 90 or a mirror 91 having a spherical dichroic mirror 93 having a property of transmitting light having a wavelength of 32 nm and reflecting light having a wavelength longer than 532 nm, and an aspheric lens 92, Further, the light having a wavelength of 473 nm is transmitted to the side of the aspherical lens 92, and
A third optical head 90 equipped with a spherical dichroic mirror 93 having a property of reflecting light having a wavelength longer than 73 nm is attached to the endless belt 66 of the scanner, and the biochemical analysis unit 1 is operated by the laser light 24. The large number of absorptive regions 4 formed on the substrate 2 are scanned to read the fluorescence data recorded on the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 for biochemical analysis. It is also possible to generate data for use.

Further, in the embodiment shown in FIGS. 16 to 21, the support 8 of the stimulable phosphor sheet 80 is used.
When reading the radiation data recorded in the stimulable phosphor layer 82 formed on 1, the mirror 91 and the aspherical lens 92 are provided, and a wavelength of 640 nm is provided on the side of the aspherical lens 92. A fourth optical head 90 equipped with a spherical dichroic mirror 93 having a property of transmitting the light of the above and reflecting only the light in the wavelength region of the photostimulated light 45 is attached to the endless belt 66 of the scanner, and Although the photostimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 is scanned by the light 24,
The mirror 91 and the aspherical lens 92 are provided, and the light having a wavelength of 640 nm is transmitted to the side of the aspherical lens 92 and only the light in the wavelength region of the stimulated emission 45 is reflected. Instead of the fourth optical head 90 having the spherical dichroic mirror 93, the mirror 36 and the aspherical lens 3 are provided.
7, and four condensing lenses 38a, 38b, 38c, 38d and 640 on the side of the aspherical lens 37.
A fourth optical head 35 including four dichroic mirrors 39a, 39b, 39c, 39d having a property of transmitting light having a wavelength of nm and reflecting only light in the wavelength region of the photostimulable light 45. Attached to the endless belt 66 of the scanner, the stimulable phosphor sheet 80 is irradiated by the laser light 24.
The radiation data recorded on the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80 is scanned by scanning the stimulable phosphor layer 82 formed on the support 81. Can also be read to generate data for biochemical analysis.

Further, in the embodiment shown in FIGS. 16 to 21, when reading fluorescence data recorded in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, The mirror 91 and the aspherical lens 9 according to the type of fluorescent substance labeling the substance of biological origin.
2 and 640 on the side of the aspherical lens 92.
a first optical head 90 and a mirror 91 having a spherical dichroic mirror 93 having a property of transmitting light having a wavelength of nm and transmitting light having a wavelength longer than 640 nm;
An aspheric lens 92 is provided, and a spherical dichroic mirror 93 having a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm is provided on the side of the aspheric lens 92. A second optical head 90 or a mirror 91 and an aspherical lens 92 are provided, and a property of transmitting light having a wavelength of 473 nm and reflecting light having a wavelength longer than 473 nm is provided to the side of the aspherical lens 92. A third optical head 90 equipped with the spherical dichroic mirror 93 is attached to the endless belt 66 of the scanner, and a large number of absorptive properties formed on the substrate 2 of the biochemical analysis unit 1 by the laser light 24. Although the area 4 is configured to be scanned, the mirror 36 is used depending on the type of fluorescent substance labeling the substance of biological origin.
And an aspherical lens 37, and further the aspherical lens 3
On the side of 7, four condenser lenses 38a, 38b, 38
c, 38d and 640 nm wavelength light are transmitted,
Has the property of reflecting light with a wavelength longer than nm 4
Sheets of dichroic mirrors 39a, 39b, 39c, 3
First optical head 35 having 9d, aspherical lens 37
To the side of the four condenser lenses 38a, 38b, 38c,
38d and 532nm wavelength light is transmitted, 532nm
4 dichroic mirrors 39a, 39b, 39c, 39d having the property of reflecting light having a longer wavelength than
Second optical head 35 or aspherical lens 3 having
On the side of 7, four condenser lenses 38a, 38b, 38
c, 38d and light having a wavelength of 473 nm are transmitted,
Has the property of reflecting light with a wavelength longer than nm 4
Sheets of dichroic mirrors 39a, 39b, 39c, 3
The third optical head 35 having 9d is attached to the endless belt 66 of the scanner, and the laser beam 24
Fluorescence data recorded on the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 by scanning the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Can also be read to generate data for biochemical analysis.

Further, in the embodiment shown in FIGS. 1 to 15, the support 11 of the stimulable phosphor sheet 10 is used.
When reading the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the mirror 36,
An aspherical lens 37 is provided, and four condenser lenses 38a, 38b, 38c, 3 are provided on the side of the aspherical lens 37.
8d and a fourth optic provided with four dichroic mirrors 39a, 39b, 39c, 39d having a property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength range of the photostimulable light 45. The head 35 is attached to an endless belt 66 of the scanner, and is configured so that the laser beam 24 scans a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. However, a mirror 36 and an aspherical lens 37 are provided, and four condenser lenses 38 are provided on the side of the aspherical lens 37.
a, 38b, 38c, 38d, and four dichroic mirrors 39a, 39 having a property of transmitting light having a wavelength of 640 nm and reflecting only light in the wavelength region of the stimulated emission 45.
Instead of the fourth optical head 35 including b, 39c, and 39d, a mirror 36 and an aspherical lens 37 are provided, and four condensing lenses 38 are provided on the side of the aspherical lens 37.
a, 38b, 38c, 38d and an optical head having four reflecting mirrors are attached to the endless belt 66 of the scanner, and a large number of laser beams 24 are formed on the support 11 of the stimulable phosphor sheet 10. The support 1 of the stimulable phosphor sheet 10 is scanned by scanning the stimulable phosphor layer region 12.
It is also possible to read the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed in No. 1 to generate biochemical analysis data.

Further, in the embodiment shown in FIGS. 1 to 15, when reading fluorescence data recorded in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, A mirror 36 and an aspherical lens 37 are provided according to the type of fluorescent substance labeling the substance derived from the living body.
And four condenser lenses 38a, 38b, 38c, 38d and 640n on the side of the aspherical lens 37.
A first optical head 35 provided with four dichroic mirrors 39a, 39b, 39c, 39d having a property of transmitting light having a wavelength of m and reflecting light having a wavelength longer than 640 nm, an aspherical lens. To the side of 37, four condensing lenses 38a, 38b, 38c and 38d and four dichroic having a property of transmitting light having a wavelength of 532 nm and reflecting light having a wavelength longer than 532 nm. Mirror 3
Four condensing lenses 38a, 38b, 38c, 38d and 473 nm are provided on the side of the second optical head 35 or the aspherical lens 37 having 9a, 39b, 39c, 39d.
The third optical head 35 including the four dichroic mirrors 39a, 39b, 39c, 39d, which has a property of transmitting light having a wavelength of 4 nm and reflecting light having a wavelength longer than 473 nm, is an endless scanner. A large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned by the laser beam 24 attached to the belt 66, but the first optical head 35 and the second optical head 3 are scanned.
Instead of the fifth and third optical heads 35, a mirror 36
And an aspherical lens 37, and further the aspherical lens 3
On the side of 7, four condenser lenses 38a, 38b, 38
An optical head equipped with c and 38d and four reflection mirrors is attached to the endless belt 66 of the scanner, and a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 by the laser light 24 are attached. A large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 by scanning
It is also possible to read the fluorescence data recorded in, and generate the data for biochemical analysis.

Further, in the embodiment shown in FIGS. 1 to 15, the first optical head 35, the second optical head 35, and the second optical head 35, depending on the type of the labeling substance labeling the substance derived from the living body, The third optical head 35 or the fourth optical head 35 is configured to be attached to the endless belt 66 of the scanner, but includes the mirror 36 and the aspherical lens 37, and further to the side of the aspherical lens 37. To four condenser lenses 38a, 38b, 38c, 38d
And four dichroic mirrors 39a, 39b, 39
The optical head 35 provided with c and 39d is fixed to the endless belt 66 of the scanner, and the dichroic mirrors 39a, 39b, 39c and 39d having different characteristics are provided according to the type of the labeling substance that labels the substance derived from the living body. , May be attached to the optical head 35.

Further, in the embodiment shown in FIGS. 16 to 21, the first optical head 90, the second optical head 90, and the second optical head 90 are selected depending on the type of the labeling substance labeling the substance derived from the living body. The third optical head 90 or the fourth optical head 90 is configured to be attached to the endless belt 66 of the scanner, but includes a mirror 91 and an aspherical lens 92, and further to the side of the aspherical lens 92. Further, the optical head 90 provided with the spherical dichroic mirror 93 is fixed to the endless belt 66 of the scanner, and depending on the type of the labeling substance that labels the substance of biological origin,
The spherical dichroic mirror 93 having different characteristics may be attached to the optical head 90.

Further, in the above-mentioned embodiment, by the radiolabeling substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1,
The stimulable phosphor contained in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 or formed on the support 81 of the stimulable phosphor sheet 80. The stimulable phosphor contained in the stimulable phosphor layer 82 is exposed to expose the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 or the stimulable phosphor of the stimulable phosphor sheet 80. Although the radiation data of the radio-labeled substance is recorded in the layer 82, the radio-labeled substance is accumulated by the radio-labeled substance selectively contained in the large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Of the stimulable phosphor contained in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 or the stimulable phosphor formed on the support 81 of the stimulable phosphor sheet 80. The stimulable phosphor contained in the stimulable phosphor layer 82. And light, the stimulable phosphor stimulable phosphor layer regions 12 or the stimulable phosphor sheet 9 of the seat 10
It is not always necessary to record radiation data on the stimulable phosphor layer 82 of 0, and a solution containing a specific binding substance is dropped onto different positions of an adsorptive substrate such as a membrane filter to obtain a large number of spots. -Like regions are formed and specific binding substances contained in a large number of spot-shaped regions are bound specifically by a substance of biological origin labeled with a radioactive labeling substance by hybridization etc. The region is selectively labeled with a radioactive labeling substance, and the stimulable phosphor sheet 10 or the stimulable phosphor sheet 80 is superposed on an absorptive substrate such as a membrane filter and selectively included in a large number of spot-like regions. The stimulable fluorescent substance contained in the stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 by the radioactive labeling substance Somatic or stimulable phosphor sheet 80
The stimulable phosphor contained in the stimulable phosphor layer 82 formed on the support 81 is exposed to expose the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 or the stimulable phosphor. Radiation data can also be recorded on the stimulable phosphor layer 82 of the body sheet 80, and the accumulation of fluorescent light in the organism or a part of the tissue of the organism to which the substance having a radioactive label is administered. The stimulable phosphor layer 82 of the stimulable phosphor sheet 80 is exposed by exposing the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 to the stimulable phosphor layer 82 of the stimulable phosphor sheet 80. Radiation data may be recorded in the stimulable phosphor layer 82 of the stimulable phosphor sheet 80, and the energy of the radiation transmitted through the subject may be stored in the stimulable phosphor layer 80 of the stimulable phosphor sheet 80. In the stimulable phosphor contained in the layer 82, By product, the stimulable phosphor sheet 80
The radiation data may be recorded in the stimulable phosphor layer 82 of the stimulable phosphor layer 82 of the stimulable phosphor sheet 10 or the stimulable phosphor layer 80 of the stimulable phosphor sheet 80. The method of recording radiation data in 82 is not particularly limited.

Further, in the above-described embodiment, the nylon 6 is embedded in the through holes 3 formed in the substrate 2 of the biochemical analysis unit 1 so as to be spaced apart from each other, and a large number of adsorbents are formed. Although the fluorescence data recorded in the active region 4 is read, the present invention is not limited to the reading of such fluorescence data, and a specific binding substance may be present at different positions of the adsorptive substrate such as a membrane filter. A large number of spot-shaped regions that have been dropped and formed are labeled with a fluorescent substance to read the recorded fluorescence data, and further, the fluorescence data such as the electrophoresis data recorded on the gel support or the transfer support. It can be widely applied to reading.

Furthermore, in the embodiment shown in FIGS. 1 to 15, the main scanning stepping motor 65 intermittently moves the optical head 35 in the main scanning direction to support the stimulable phosphor sheet 10. A large number of stimulable phosphor layer regions 12 formed on the body 11 or a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are configured to be scanned by the laser light 24. However, similar to the embodiment shown in FIGS. 16 to 21, the optical head 90 is driven by the main scanning motor 95.
Are continuously moved in the main scanning direction, and a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are scanned by the laser light 24. You can also

In the embodiment shown in FIGS. 16 to 21, the main scanning motor 95 continuously moves the optical head 90 in the main scanning direction to support the stimulable phosphor sheet 80. The laser beam 24 is applied to the exposure region 85 formed on the stimulable phosphor layer 82 formed on one surface of the laser beam 81 or the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. However, in the same manner as the embodiment shown in FIGS. 1 to 15, the main scanning stepping motor 65 is used.
Thus, the optical head 35 is intermittently moved in the main scanning direction to form an exposure area on the stimulable phosphor layer 82 formed on one surface of the support 81 of the stimulable phosphor sheet 80. It is also possible to configure 85 to be scanned by the laser light 24.

Further, in the above embodiment, the optical head 35 or the optical head 90 is used depending on the scanning mechanism.
Are moved in the main scanning direction and the sub-scanning direction by the laser beam 34, and all the stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 and the stimulable phosphor of the stimulable phosphor sheet 80 are moved. The entire surface of the layer 82 or all the absorptive regions 4 of the biochemical analysis unit 1 are scanned to excite a fluorescent substance such as a stimulable phosphor or a fluorescent dye. By maintaining the stationary state and moving the stage 40 in the main scanning direction and the sub scanning direction, all the stimulable phosphor layer regions 1 of the stimulable phosphor sheet 10 are irradiated with the laser light 34.
2. The entire surface of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80 or all the absorptive regions 4 of the biochemical analysis unit 1 are scanned to detect a stimulable phosphor or a fluorescent substance such as a fluorescent dye. The optical head 35 or the optical head 90 may be moved in the main scanning direction or the sub scanning direction, and the stage 40 may be moved in the sub scanning direction or the main scanning direction. By the laser light 34, all of the stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10, the entire surface of the stimulable phosphor layer 82 of the stimulable phosphor sheet 80, or all of the biochemical analysis unit 1. The absorptive region 4 may be scanned to excite a fluorescent substance such as a stimulable phosphor or a fluorescent dye.

Further, in the above-mentioned embodiment, the scanner uses the radiation data of the radio-labeled substance recorded in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the radiation data. Storage phosphor sheet 80
Radiation data recorded in the stimulable phosphor layer 82 formed on the support 81, and fluorescence data recorded in many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. 640n
A first laser pumping light source 21 that emits a laser beam 24 having a wavelength of m and a second laser beam that emits a laser beam 24 having a wavelength of 532 nm.
The laser excitation light source 22 and the third laser excitation light source 23 that emits the laser light 24 having a wavelength of 473 nm are provided.
Data of the radio-labeled substance recorded in the large number of stimulable phosphor layer regions 12 formed on the substrate and recorded in the stimulable phosphor layer 82 formed on the support 81 of the stimulable phosphor sheet 80. It is not always necessary that the radiation data stored therein and the fluorescence data recorded in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 can be read.
First laser excitation light source 21 for emitting laser light 24 having a wavelength of 0 nm, second laser excitation light source 22 for emitting laser light 24 having a wavelength of 532 nm, and third laser excitation light source emitting laser light 24 for a wavelength of 473 nm It is not always necessary to have 23.

Further, in the above embodiment, the scanner includes the first laser excitation light source 21 which emits the laser light 24 having the wavelength of 640 nm and the laser light 2 having the wavelength of 532 nm.
Second laser excitation light source 22 which emits 4 and third laser excitation light source 23 which emits laser light 24 having a wavelength of 473 nm.
However, it is not always necessary to use a laser excitation light source as the excitation light source, and instead of the laser excitation light source, an LED light source can be used as the excitation light source, and further, a halogen lamp can be used as the excitation light source. Used as
A spectral filter may be used to cut wavelength components that do not contribute to excitation of the stimulable phosphor.

Further, in the above-mentioned embodiment, the scanner is equipped with the photomultiplier 50 as a photodetector for photoelectrically detecting photostimulation light or fluorescence, but as a photodetector used in the present invention, Any photodetector such as a line CCD or a two-dimensional CCD can be used instead of the photomultiplier 50 as long as it can photoelectrically detect stimulated emission or fluorescence.

[0349]

EFFECTS OF THE INVENTION According to the present invention, with a simple structure, the excitation light excites the photostimulable phosphor or fluorescent substance contained in the sample to emit light omnidirectionally efficiently. It is possible to provide a scanner that is good, focused, sensitive and capable of producing data.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic vertical sectional view of a hybridization reaction container.

FIG. 4 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 5 is a schematic diagram showing a large number of radiolabeled substances formed on a support of a stimulable phosphor sheet by a radioactive labeling substance selectively contained in a large number of absorptive regions formed on a substrate of a biochemical analysis unit. FIG. 3 is a schematic cross-sectional view showing a method of exposing the photostimulable phosphor layer region of FIG.

FIG. 6 is a diagram showing radiation data of radiolabeled substances recorded in a large number of stimulable phosphor layer regions formed on a support of a stimulable phosphor sheet and formed on a substrate of a biochemical analysis unit. FIG. 6 is a schematic perspective view of a scanner that reads fluorescence data such as a fluorescent dye recorded in a large number of absorptive regions that have been created and generates biochemical analysis data.

7 is a schematic perspective view showing details near the photomultiplier of the scanner shown in FIG. 6;

FIG. 8 is a schematic sectional view of an optical head.

FIG. 9 is a schematic bottom view of the optical head.

10 is a schematic cross-sectional view taken along the line AA of FIG.

11 is a cross-sectional view taken along the line BB of FIG.

12 is a cross-sectional view taken along the line CC of FIG.

13 is a cross-sectional view taken along the line DD of FIG.

FIG. 14 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 15 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner according to the preferred embodiment of the present invention.

FIG. 16 is a schematic perspective view showing another example of the stimulable phosphor sheet.

FIG. 17 is a diagram showing that a radioactive labeling substance selectively contained in a large number of absorptive regions formed on the substrate of the biochemical analysis unit is formed on the surface of the support of the stimulable phosphor sheet. It is a schematic sectional drawing which shows the method of exposing the stimulable phosphor contained in the stimulable phosphor layer.

FIG. 18 shows photostimulability by an electron beam (β-ray) emitted from a radiolabeled substance selectively contained in many absorptive regions formed on a substrate of a biochemical analysis unit. It is a schematic perspective view of the stimulable phosphor sheet after the stimulable phosphor contained in the phosphor layer is exposed.

FIG. 19 is a schematic bottom view of an optical head provided in the scanner provided in the scanner according to another preferred embodiment of the present invention.

20 is a schematic cross-sectional view taken along the line EE of FIG.

FIG. 21 is a block diagram showing a control system, an input system, a drive system and a detection system of a scanner according to another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical Analysis Unit 2 Substrate 3 Through Hole 4 Adsorbent Region 5 Injector 6 CCD Camera 8 Hybridization Reaction Container 9 Hybridization Reaction Solution 10 Accumulative Phosphor Sheet 11 Support 12 Photostimulable Phosphor Layer Region 13 Through Hole 21 First Laser Excitation Light Source 22 Second Laser Excitation Light Source 23 Third Laser Excitation Light Source 24 Laser Light 25 Collimator Lens 26 Mirror 27 First Dichroic Mirror 28 Second Dichroic Mirror 29 Mirror 30 Collimator Lens 31 Collimator Lens 32 Mirror 33 Perforated mirror 33a Perforated mirror hole 34 Concave mirror 35 Optical head 36 Mirror 37 Aspherical lenses 38a, 38b, 38c, 38d Condensing lenses 39a, 39b, 39c, 39d Dichroic mirror 40 Stage 41 Glass plate 45 Fluorescent or Excited Light 48 Filter Unit 50 Photomultipliers 51a, 51b, 51c, 51d Filter Members 52a, 52b, 52c, 52d Filter 53 A / D Converter 54 Data Processing Device 60 Substrate 61 Sub-scanning Pulse Motor 62 Rail 63 Movable substrate 64 Rod 65 Main scanning stepping motor 66 Endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 Keyboard 72 Filter unit motor 80 Accumulable phosphor sheet 81 Support 82 Stimulable phosphor layer 85 Exposure area 90 Optical head 91 Mirror 92 Aspherical lens 93 Spherical dichroic mirror 95 Main scanning motor

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) H04N 1/04 H04N 1/04 E 5C072 F term (reference) 2G043 AA01 BA16 CA03 DA02 EA01 FA01 FA06 GA02 GA04 GB01 GB18 GB19 HA01 HA02 HA09 JA03 KA02 KA05 KA09 LA03 2G054 AA06 AA10 EA03 GA04 GB02 GE01 GE07 2H013 AC03 AC04 AC05 2H087 KA19 RA44 RA45 RA48 TA02 TA06 5C051 AA01 BA01 DA02 CA04 DA01 CA01 FA04 BA02 FA05 5C072

Claims (5)

[Claims] 1. An excitation light source that emits excitation light, a sample stage on which a sample is placed, and the excitation light emitted from the excitation light source scans the sample and collects the light emitted from the sample. Focusing optical system, a photodetector for photoelectrically detecting the light emitted from the sample and focused by the focusing optical system, the sample stage, and the optical path of the excitation light emitted from the excitation light source. And scanning means for relatively moving the condensing optical system in the main scanning direction and a sub-scanning direction orthogonal to the main scanning direction, and a side of the condensing optical system, and the excitation light is irradiated. Is provided in the vicinity of the region of the sample to be emitted, and the light emitted from the sample is directed toward the sample,
A reflection optical system for reflecting light, and the reflection optical system is configured to be relatively moved by the scanning means in the main scanning direction and the sub-scanning direction with respect to the sample stage. Characteristic scanner. 2. The main scanning means, wherein the scanning means relatively intermittently moves the sample stage, the optical path of the excitation light emitted from the excitation light source, and the condensing optical system in the main scanning direction. 2. The scanner of claim 1, wherein the scanner is configured to reflect light emitted from the sample toward an area of the sample from which the light was emitted. 3. The dichroic mirror having a property of transmitting the light having the wavelength of the excitation light and reflecting the light emitted from the sample, according to claim 1 or 2. The listed scanner. 4. The spherical optical dichroic mirror having a property of transmitting the light having the wavelength of the excitation light and reflecting the light emitted from the sample, according to claim 3. Scanner. 5. A plurality of dichroic mirrors having a property that the reflection optical system transmits light having a wavelength of excitation light and reflects light emitted from the sample, and the dichroic light emitted from the sample. 4. A plurality of condensing lenses for condensing the light emitted from the sample and reflected by the dichroic mirror to the sample while being guided to a mirror, and further comprising: a plurality of condensing lenses. Scanner as described in.