JP2003075440A - Unit for biochemical analysis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a unit for a biochemical analysis that densely forms a plurality of spot-like regions containing a specific combination substance on a substrate, specifically combines a substance originated from an organism that is labeled by a radioactive label to the specifically combined substance, adheres the unit for a biochemical analysis obtained by selectively labeling the plurality of spot-like regions to a phosphor layer having an accelerated phosphorescence, exposes the phosphor layer having an accelerated phosphorescence by the radioactive labeled substance, applies excitation light to the phosphor layer having an accelerated phosphorescence, photoelectrically detects light having an accelerated phosphorescence radiated from the phosphor layer having an accelerated phosphorescence, generates data for a biochemical analysis, and prevents noise caused by the scattering in electron rays (β rays) emitted from the radioactive labeled substance when analyzing a substance originated from the organism. SOLUTION: The unit 1 for a biochemical analysis is formed by a material having a property for attenuating radiation energy and/or light energy, has a substrate 2 where a plurality of holes 3 are formed separated one another and an attraction layer 4 is formed on an inner surface 3a of the plurality of holes.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a biochemical analysis unit, and more specifically, it is capable of specifically binding to a substance derived from a living body and has a base sequence, a base length, a composition, etc. , A plurality of spot-shaped regions containing known specific binding substances are formed at high density on the substrate, and the specific binding substances contained in the plurality of spot-shaped regions are labeled with a radioactive labeling substance of biological origin. A biochemical analysis unit obtained by selectively binding multiple spot-shaped regions by specifically binding a substance is brought into close contact with the stimulable phosphor layer to make the stimulable phosphor layer radioactive. Exposing with a labeling substance, irradiating the stimulable phosphor layer with excitation light, photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer, and generating biochemical analysis data, Even when analyzing a substance derived from a living body, It is possible to prevent the generation of noise in the data for biochemical analysis, which is caused by the scattering of the generated electron beam (β-ray), and it is possible to specifically bind to the substance of biological origin and the base sequence. Multiple spot-like regions containing specific binding substances of known base length, composition, etc.
A substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting a chemiluminescent substrate with a specific binding substance formed on a substrate at high density and contained in multiple spot-shaped regions A chemiluminescent substrate is brought into contact with a biochemical analysis unit obtained by selectively labeling a plurality of spot-shaped regions with a chemiluminescent substrate to emit chemiluminescence, and the biochemical analysis releases chemiluminescence. Unit for
The photostimulable phosphor layer is brought into close contact with the photostimulable phosphor layer, and the photostimulable phosphor layer is exposed by chemiluminescence. Even when analyzing biogenic substances by photoelectrically detecting exhaustion and generating biochemical analysis data, noise caused by chemiluminescence scattering should be generated in the biochemical analysis data. In addition, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. are formed on the substrate. To
Chemiluminescence is generated by contacting a specific binding substance formed in high density and contained in a plurality of spot-like regions with a chemiluminescent substrate in addition to or in place of the radiolabeled substance. A substance derived from a living body labeled with a labeling substance and / or a fluorescent substance is specifically bound, and chemiluminescence or fluorescence emitted from a biochemical analysis unit obtained by selectively labeling is photoelectrically detected. When generating biochemical analysis data and analyzing substances derived from living organisms, chemiluminescence emitted from a labeled substance or fluorescence emitted from a fluorescent substance that causes chemiluminescence when contacted with a chemiluminescent substrate TECHNICAL FIELD The present invention relates to a biochemical analysis unit capable of preventing generation of noise in biochemical analysis data due to scattering of light.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Furthermore, in recent years, cells at different positions on the surface of a carrier such as a slide glass plate or a membrane filter,
Viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA
A specific binding substance, such as A, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Forming a large number of independent spots, then cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c
A substance derived from a living body, such as DNA, DNA, or mRNA, which is collected from the living body by extraction or isolation, or which is further subjected to a chemical treatment, a chemical modification, or the like, such as a fluorescent substance or a dye. A substance labeled with a labeling substance is bound to a specific binding substance by hybridization or the like, to a microarray that is specifically bound,
A microarray analysis system has been developed which irradiates excitation light and photoelectrically detects light such as fluorescence emitted from a labeling substance such as a fluorescent substance or a dye to analyze a substance derived from a living body. According to this microarray analysis system,
A large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and the substance of biological origin labeled with the labeling substance is hybridized, thus In time, there is an advantage that it is possible to analyze a substance of biological origin.

In addition, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, RNAs, etc. derived from living organisms are located at different positions on the surface of a carrier such as a membrane filter. The specific binding substance that can specifically bind to the substance of which the base sequence, base length, composition, etc. are known is dropped using a spotter device to form a large number of independent spots. , Then, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc., collected from the living body by extraction or isolation, or
Furthermore, a substance derived from a living body, which has been subjected to a chemical treatment, a chemical modification, or the like, and which is labeled with a radioactive labeling substance, is specifically bound to a specific binding substance by hybridization or the like. Macro array,
The stimulable phosphor layer containing the stimulable phosphor is brought into close contact with the stimulable phosphor sheet, and the stimulable phosphor layer is exposed.
After that, the photostimulable phosphor layer is irradiated with excitation light, the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected, and biochemical analysis data is generated. A macroarray analysis system using a radiolabeled substance for analyzing is also developed.

[0008]

However, in a macro array analysis system using a radioactive labeling substance as a labeling substance, when the stimulable phosphor layer is exposed, it is formed on the surface of a carrier such as a membrane filter. Since the radiation energy of the radio-labeled substance contained in the spot-shaped area is very large, the electron beam (β-ray) emitted from the radio-labeled substance is scattered and the radio-labeled substance contained in the adjacent spot-shaped area The electron beam (β-ray) emitted from the radiolabeled substance incident on the region of the stimulable phosphor layer to be exposed or adhering to the surface of a carrier such as a membrane filter between adjacent spot-shaped regions Then, noise is generated in the data for biochemical analysis generated by photoelectrically detecting the stimulable stimulus light, and noise is generated in the adjacent spots. It becomes difficult to separate the data between the spot-shaped regions, which lowers the resolution, and when the amount of radiation in each spot-shaped region is quantified and the substance derived from the living body is analyzed, the quantitativeness deteriorates. However, when spot-like regions are formed close to each other in order to increase the density, the resolution is remarkably lowered and the quantitativeness is remarkably deteriorated.

In order to prevent such noise by preventing noise caused by scattering of electron beams (β-rays) emitted from radiolabeled substances contained in adjacent spot-shaped regions,
Inevitably, it is necessary to increase the distance between adjacent spot-shaped areas, which reduces the density of spot-shaped areas.
There was a problem of reducing the inspection efficiency.

In the field of biochemical analysis, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c at different positions on the surface of a carrier such as a membrane filter.
Multiple spot-like regions are formed that can specifically bind to substances of biological origin such as DNA, DNA, RNA, and that contain specific binding substances with known base sequences, base lengths, compositions, etc. Substance and / or fluorescent substance that causes chemiluminescence by contacting with a chemiluminescent substrate in addition to or instead of the radiolabeled substance in the specific binding substance contained in the spot-shaped region of The substance of biological origin labeled by is specifically bound by hybridization or the like to selectively label a plurality of spot-like regions, and the chemiluminescent substrate is brought into contact with the chemiluminescent substrate and the labeling substance. Photoelectrically detect chemiluminescence in the visible wavelength range caused by contact with
Irradiating excitation light to a plurality of spot-shaped regions, photoelectrically detecting fluorescence emitted from a fluorescent substance, it is also required to analyze a substance derived from a living body, but even in such a case,
Chemiluminescence or fluorescence emitted from multiple spot-shaped regions is scattered in a carrier such as a membrane filter, or
Chemiluminescence or fluorescence emitted from a spot-shaped area is scattered and mixed with chemiluminescence or fluorescence emitted from an adjacent spot-shaped area, and as a result, chemiluminescence or fluorescence is photoelectrically detected and generated. There is a problem that noise is generated in the biochemical analysis data.

Further, in the field of biochemical analysis, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c at different positions on the surface of a carrier such as a membrane filter.
A spot-like region is formed by forming a spot-like region that can specifically bind to a substance of biological origin such as DNA, DNA, and RNA and that contains a specific binding substance whose base sequence, base length, composition, etc. are known. To the specific binding substance contained in, a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is specifically bound by hybridization or the like,
The stimulable phosphor layer of the stimulable phosphor sheet is exposed by selective labeling, contact with the chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by contact between the chemiluminescent substrate and the labeling substance. Energy is accumulated in the stimulable phosphor layer, the stimulable phosphor layer is irradiated with excitation light, and the stimulable light emitted from the stimulable phosphor layer is photoelectrically detected. Then, it is also required to analyze the substance derived from the living body, but even in such a case, the chemiluminescence emitted from each spot-shaped region is scattered in the carrier such as the membrane filter and the adjacent spot-shaped regions are scattered. Incident on the region of the stimulable phosphor layer to be exposed by the chemiluminescence emitted from the region,
As a result, noise is generated in the biochemical analysis data generated by photoelectrically detecting the stimulated luminescence, and it becomes difficult to separate the data between adjacent spot-like regions, and the resolution is reduced. However, there is a problem that the quantitativeness of the biochemical analysis data decreases.

Therefore, according to the present invention, a plurality of spot-shaped regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. are formed on the substrate. In addition, a substance of biological origin labeled with a radioactive labeling substance is specifically bound to a specific binding substance that is formed at high density and that is contained in multiple spot-shaped regions to selectively select multiple spot-shaped regions. The biochemical analysis unit obtained by labeling the stimulable phosphor layer is brought into close contact with the stimulable phosphor layer, and the stimulable phosphor layer is exposed to a radioactive labeling substance, and the stimulable phosphor layer is irradiated with excitation light. Then, photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor layer to generate data for biochemical analysis, and when analyzing a substance derived from a living body, electrons emitted from the radiolabeled substance Noise due to scattering of X-rays (β-rays) for biochemical analysis It is an object to provide a biochemical analysis unit which can be prevented from being produced during chromatography data.

Another object of the present invention is to provide a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like. , Specific binding substance that is formed on the substrate at high density and that causes chemiluminescence by contacting the specific binding substance contained in multiple spot-like regions with the chemiluminescent substrate Chemiluminescence is released by contacting a chemiluminescent substrate with a biochemical analysis unit obtained by selectively labeling multiple spot-like regions by chemically binding them. The analysis unit is brought into close contact with the stimulable phosphor layer to form the stimulable phosphor layer.
Exposing by chemiluminescence, irradiating the stimulable phosphor layer with excitation light, photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate biochemical analysis data, An object of the present invention is to provide a biochemical analysis unit capable of preventing noise caused by chemiluminescence scattering from being generated in biochemical analysis data even when analyzing a biological substance. It is a thing.

Still another object of the present invention is to provide a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition and the like. By forming a high density on a substrate and contacting a specific binding substance contained in a plurality of spot-like regions with a chemiluminescent substrate in addition to the radiolabeling substance or in place of the radiolabeling substance. A substance derived from a living body, which is labeled with a labeling substance and / or a fluorescent substance that causes chemiluminescence, is specifically bound to selectively label and obtain the chemiluminescence or fluorescence emitted from the biochemical analysis unit. Released from labeled substances that generate chemiluminescence when contacted with a chemiluminescent substrate, even when biochemical analysis data is generated and biogenic substances are analyzed In that the noise caused by the scattering of the fluorescence emitted from the academic luminescent or fluorescent material to provide a biochemical analysis unit which can be prevented from being generated during biochemical analysis data.

[0015]

The object of the present invention is to:
A substrate formed of a material having a property of attenuating radiation energy and / or light energy and having a plurality of holes separated from each other, wherein an adsorption layer is formed on an inner surface of the plurality of holes. It is achieved by the unit for biochemical analysis.

According to the present invention, when the substrate is made of a material having a property of attenuating radiation energy, it can specifically bind to a substance of biological origin and has a base sequence and a base length, A specific binding substance with a known composition etc. is dropped into a plurality of holes formed in the biochemical analysis unit, adsorbed on the adsorption layer formed on the inner surface of the plurality of holes, and labeled with a radioactive labeling substance. The bio-derived substance is used for biochemical analysis after it is selectively bound to the specific binding substance adsorbed in the multiple adsorption layers by hybridization, etc. and the multiple adsorption layers are selectively labeled. When exposing the photostimulable phosphor layer to the unit with the unit facing the stimulable phosphor layer, an electron beam (β-ray) emitted from the radiolabeled substance adsorbed on the plurality of adsorption layers Is radiation Since the energy is attenuated by the substrate having the property of attenuating the energy, the electron beam (β-ray) is scattered in the substrate of the biochemical analysis unit, and the regions of the stimulable phosphor layer facing each adsorption layer are adjacent to each other. The region of the stimulable phosphor layer facing each adsorption layer can be effectively prevented from being exposed by the electron beam (β-ray) emitted from the adsorption layer in the matching hole and scattered in the substrate. Electron beam (β
Line) makes it possible to selectively expose the photostimulable phosphor layer exposed to the radiolabeled substance to the photostimulable phosphor layer by irradiating the photostimulable phosphor layer with excitation light. Even when light is photoelectrically detected to generate biochemical analysis data and a substance derived from a living body is analyzed, noise caused by scattering of an electron beam (β-ray) emitted from a radiolabeled substance is biochemical. It is possible to effectively prevent generation in the analysis data.

Furthermore, according to the present invention, when the substrate is made of a material having a property of attenuating light energy, it can specifically bind to a substance of biological origin and has a base sequence or a base length. A specific binding substance having a known composition is dropped into a plurality of holes formed in the biochemical analysis unit and adsorbed on the adsorption layer formed on the inner surface of the plurality of holes to form a chemiluminescent substrate. A substance derived from a living body that is labeled with a labeling substance that causes chemiluminescence when brought into contact with a substance specifically binds to a specific binding substance adsorbed in the adsorption layer, and is selectively labeled. The analytical unit is brought into contact with a chemiluminescent substrate to selectively emit chemiluminescence from a plurality of adsorption layers, and the biochemical analytical unit is made to face the stimulable phosphor layer to be stimulated by chemiluminescence. Fluorescent phosphor layer When light is emitted, chemiluminescence selectively emitted from the plurality of adsorption layers is attenuated by the substrate having a property of attenuating light energy, and thus chemiluminescence is scattered in the substrate of the biochemical analysis unit, The region of the stimulable phosphor layer facing each adsorption layer can be effectively prevented from being emitted from the adsorption layer in the adjacent pores and exposed by the chemiluminescence scattered in the substrate, Only the region of the stimulable phosphor layer facing each adsorption layer can be selectively exposed by chemiluminescence, and therefore, excitation light is emitted to the stimulable phosphor layer exposed by chemiluminescence. Scattering of chemiluminescence even when irradiating and stimulating photostimulated light emitted from the stimulable phosphor layer photoelectrically to generate data for biochemical analysis and analyzing a substance derived from a living body. Noise caused by is in biochemical analysis data It is possible to effectively prevented from being generated.

Further, according to the present invention, when the substrate is made of a material having a property of attenuating light energy, it can specifically bind to a substance of biological origin, and
A specific binding substance with a known base sequence, base length, composition, etc. is dropped into the plurality of holes formed in the biochemical analysis unit to form an adsorption layer formed on the inner surface of the plurality of holes. Specific binding of a biological substance labeled with a labeling substance and / or a fluorescent substance that causes chemiluminescence by contacting with a chemiluminescent substrate instead of the radioactive labeling substance After specifically binding to a substance and selectively labeling, the biochemical analysis unit is brought into contact with a chemiluminescent substrate, and chemiluminescence generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected,
Alternatively, by irradiating the biochemical analysis unit with excitation light and photoelectrically detecting fluorescence emitted from the fluorescent substance, when generating biochemical analysis data, a substrate having a property of attenuating light energy, Since the chemiluminescence or fluorescence is attenuated, it is possible to effectively prevent the chemiluminescence or fluorescence emitted from the adsorption layer from being scattered in the substrate of the biochemical analysis unit, and thus the chemiluminescence or fluorescence is prevented. It is possible to effectively prevent the generation of noise due to chemiluminescence or fluorescence scattering in the biochemical analysis data generated by photoelectric detection.

Furthermore, according to the present invention, when the substrate is made of a material having a property of attenuating radiation and light energy, it can specifically bind to a substance of biological origin and has a base sequence or base. Specific binding substances of known length, composition, etc. are dropped into the multiple holes formed in the biochemical analysis unit, and are adsorbed to the adsorption layer formed on the inner surface of the multiple In addition to the labeling substance, a substance derived from a living body labeled with a labeling substance and / or a fluorescent substance that produces chemiluminescence by contacting with a chemiluminescent substrate is specific to the specific binding substance adsorbed in the adsorption layer. When the photostimulable phosphor layer is exposed to the radioactive labeling substance, the biochemical analysis unit faces the photostimulable fluorescent substance layer, and the biochemical analysis unit is exposed to the radioactive labeling substance. Because was electron beam (beta ray) is attenuated by the substrate of the biochemical analysis unit, an electron beam (beta
It is possible to effectively prevent the light rays) from being scattered in the substrate, and to expose only the region of the stimulable phosphor layer facing each adsorption layer with an electron beam (β ray). Therefore, the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected to generate the biochemical analysis. Electron beam (β-ray) emitted from radiolabeled substances
It becomes possible to effectively prevent the noise due to the scattering of the substances, while the substance of biological origin is specifically bound to the specific binding substance, selectively labeled, and then contacted with the chemiluminescent substrate. Then, the chemiluminescence generated by the contact between the chemiluminescent substrate and the labeling substance is photoelectrically detected, or the biochemical analysis unit is irradiated with excitation light and the fluorescence emitted from the fluorescent substance is photoelectrically detected. , Because the substrate is made of a material that has the property of attenuating radiation and light energy when generating data for biochemical analysis,
It is possible to effectively prevent scattering of chemiluminescence or fluorescence in the substrate. Therefore, in the data for biochemical analysis generated by photoelectrically detecting chemiluminescence or fluorescence, it is possible to scatter chemiluminescence or fluorescence. It is possible to effectively prevent the generation of noise due to.

The above object of the present invention also includes a substrate formed of a material that attenuates radiation energy and / or light energy, the substrate having a plurality of holes spaced apart from each other, and the plurality of substrates formed on the substrate. A plurality of adsorption layers are formed on the inner surface of the pores, and the plurality of adsorption layers are at least selected from the group consisting of radioactive labeling substances, fluorescent substances, and labeling substances that generate chemiluminescence by contacting with chemiluminescent substrates. This is achieved by a biochemical analysis unit characterized by being selectively labeled with one type of labeling substance.

According to the present invention, when the substrate of the biochemical analysis unit is formed of a material that attenuates radiation energy and a plurality of adsorption layers are selectively labeled with a radioactive labeling substance, the biochemistry is improved. When exposing the stimulable phosphor layer to the stimulable phosphor layer with the analytical unit facing the stimulable phosphor layer, the electron beam emitted from the radiolabeled substance adsorbed by the plurality of adsorption layers (β (Rays) are attenuated by the substrate that has the property of attenuating radiation energy, electron beams (β-rays) are scattered within the substrate of the biochemical analysis unit, and the stimulable phosphor layer facing each adsorption layer. This region can be effectively prevented from being emitted from the adsorbing layers in the adjacent holes and being exposed by the electron beam (β-ray) scattered in the substrate. Area of fluorescent phosphor layer , It becomes possible to selectively expose the photostimulable phosphor layer exposed by the radiolabeling substance to the photostimulable phosphor layer by irradiating it with excitation light. When the emitted photostimulant light is photoelectrically detected to generate biochemical analysis data and the substance derived from the living body is analyzed, it is caused by the scattering of the electron beam (β-ray) emitted from the radiolabeled substance. It is possible to effectively prevent the generated noise from being generated in the biochemical analysis data.

Furthermore, according to the present invention, the label of the substrate of the biochemical analysis unit is formed of a material that attenuates light energy, and a plurality of adsorption layers are brought into contact with a chemiluminescent substrate to generate chemiluminescence. Depending on the substance
When selectively labeled, the biochemical analysis unit is brought into contact with a chemiluminescent substrate to selectively emit chemiluminescence from a plurality of adsorption layers, and the biochemical analysis unit is used as a stimulable phosphor layer. The chemiluminescence emitted from the plurality of adsorption layers when the stimulable phosphor layer is exposed by the chemiluminescence facing each other is attenuated by the substrate having the property of attenuating the light energy. Are scattered within the substrate of the biochemical analysis unit, and the regions of the stimulable phosphor layer facing each adsorption layer are emitted from the adsorption layer in the adjacent holes and exposed by the chemiluminescence scattered in the substrate. Can be effectively prevented, and only the region of the stimulable phosphor layer facing each adsorption layer can be selectively exposed by chemiluminescence, and therefore, it can be exposed by chemiluminescence. Exposed photostimulable firefly Irradiating the body layer with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate data for biochemical analysis and also when analyzing a substance derived from a living body. Therefore, it becomes possible to effectively prevent the noise caused by the scattering of chemiluminescence from being generated in the biochemical analysis data.

Further, according to the present invention, the substrate of the biochemical analysis unit is formed of a material that attenuates light energy, and a plurality of adsorption layers are brought into contact with a fluorescent substance and / or a chemiluminescent substrate to produce a chemical substance. When selectively labeled with a labeling substance that produces luminescence, the biochemical analysis unit is brought into contact with a chemiluminescent substrate, and chemiluminescence generated by the contact between the chemiluminescent substrate and the labeling substance is photoelectrically detected, Alternatively, by irradiating the biochemical analysis unit with excitation light and photoelectrically detecting fluorescence emitted from the fluorescent substance, when generating biochemical analysis data, a substrate having a property of attenuating light energy, Since the chemiluminescence or fluorescence is attenuated, it is effective that the chemiluminescence or fluorescence emitted from the adsorption layer is scattered within the substrate of the biochemical analysis unit. Therefore, it is possible to effectively prevent generation of noise due to scattering of chemiluminescence or fluorescence in the data for biochemical analysis generated by photoelectrically detecting chemiluminescence or fluorescence. It becomes possible to prevent.

Furthermore, according to the present invention, the substrate of the biochemical analysis unit is formed of a material that attenuates radiation energy and light energy, and a plurality of adsorption layers are added to the radioactive labeling substance, and a fluorescent substance and / or Alternatively, when selectively labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate, the biochemical analysis unit is made to face the stimulable phosphor layer to form a stimulable phosphor layer. The electron beam (β-ray) emitted from the radioactive-labeled substance is attenuated by the substrate of the biochemical analysis unit when the substance is exposed to the radioactive-labeled substance.
It is possible to effectively prevent the light rays) from being scattered in the substrate, and to expose only the region of the stimulable phosphor layer facing each adsorption layer with an electron beam (β ray). Therefore, the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected to generate the biochemical analysis. Electron beam (β-ray) emitted from radiolabeled substances
It is possible to effectively prevent the noise due to the scattering of light, while the biochemical analysis unit is brought into contact with the chemiluminescent substrate, and the chemistry in the visible light wavelength region caused by the contact between the chemiluminescent substrate and the labeling substance is generated. When the luminescence is detected photoelectrically, or when the biochemical analysis unit is irradiated with excitation light and the fluorescence emitted from the fluorescent substance is photoelectrically detected to generate biochemical analysis data, the substrate is Since it is formed of a material having a property of attenuating radiation and light energy, it is possible to effectively prevent chemiluminescence or fluorescence from scattering in the substrate, and therefore, chemiluminescence or fluorescence can be detected photoelectrically. It is possible to effectively prevent generation of noise due to chemiluminescence or fluorescence scattering in the generated biochemical analysis data.

In the present invention, preferably, a plurality of adsorption layers are adsorbed with a specific binding substance having a known base sequence, base length, composition, etc., and a substance derived from a living body labeled with a radioactive labeling substance is By specifically binding to the specific binding substance, the plurality of adsorption layers of the biochemical analysis unit are selectively labeled with the radioactive labeling substance.

In the present invention, the phrase "the plurality of adsorption layers of the biochemical analysis unit are selectively labeled with a fluorescent substance" means that a substance of biological origin labeled with a fluorescent dye is attached to the plurality of adsorption layers. The specific binding substances included are:
By selectively binding, the plurality of adsorption layers are selectively labeled with a fluorescent substance, and the specific binding substance contained in the plurality of adsorption layers is labeled with a hapten from a living body. By selectively binding a substance and further contacting it with a fluorescent substrate, an antibody against the hapten labeled with an enzyme having a property of producing a fluorescent substance is bound to the hapten by an antigen-antibody reaction,
The case where a plurality of adsorption layers are selectively labeled with a fluorescent substance by contacting an enzyme bound to a hapten with a fluorescent substrate to generate a fluorescent substance is included.

Further, in the present invention, the term “selectively labeled with a labeling substance capable of producing chemiluminescence by contacting a plurality of adsorption layers of a biochemical analysis unit with a chemiluminescent substrate” means chemiluminescence. A substance derived from a living body, which is labeled with a labeling substance that causes chemiluminescence when brought into contact with a substrate, is selectively bound to a specific binding substance contained in the plurality of adsorption layers to form a plurality of adsorption layers. Is selectively labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate, and a specific binding substance contained in a plurality of adsorption layers is labeled with a hapten in a living body. To a hapten labeled with an enzyme that selectively binds the substance from which it originates and then produces chemiluminescence by contacting it with a chemiluminescent substrate A case in which a plurality of adsorption layers are selectively labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate by binding an antibody to a hapten by an antigen-antibody reaction. ing.

In the present invention, examples of the hapten / antibody combination include digoxigenin / anti-digoxigenin antibody, theophylline / anti-theophylline antibody, fluorescein / anti-fluorescein antibody and the like.
It is also possible to use a combination of biotin / avidin or antigen / antibody instead of the hapten / antibody.

In a preferred aspect of the present invention, the plurality of holes are formed by recesses.

According to a preferred embodiment of the present invention, the adsorption layer is formed on the inner surfaces of the plurality of recesses formed in the substrate,
Since the specific binding substance that is a probe is adsorbed in a small volume region, it is possible to improve the reaction rate of a specific binding reaction such as hybridization, and further, a solution containing a substance derived from a living body, Since each adsorption layer can be brought into contact with a sufficiently large surface area, it is possible to increase the probability that the target biological substance will encounter the specific binding substance that is the probe adsorbed in the adsorption layer. Therefore, the efficiency of a specific binding reaction such as hybridization can be significantly improved.

Further, according to a preferred embodiment of the present invention, the specific binding substance is adsorbed on the adsorption layer formed on the inner surfaces of the plurality of recesses formed on the substrate and labeled with the labeling substance. The derived substance is also hybridized with the specific binding substance contained in the adsorption layer. Therefore, when cleaning the biochemical analysis unit, the adsorption layer formed on the inner surface of the recess is washed. Since the adsorption layer has a large surface area, the biochemical analysis unit can be efficiently washed and reused.

In another preferred embodiment of the present invention, the plurality of holes are formed by through holes.

According to another preferred embodiment of the invention,
An adsorption layer is formed on the inner surface of the plurality of through-holes formed on the substrate, and the specific binding substance, which is a probe, is adsorbed in the area of a small volume, so that the reaction of the specific binding reaction such as hybridization occurs. It is possible to improve the speed, and furthermore, a solution containing a substance derived from a living body can be brought into contact with each adsorption layer in a sufficiently large area. It is possible to increase the probability of encountering a specific binding substance that is a probe that is being used, and thus it is possible to greatly improve the efficiency of a specific binding reaction such as hybridization.

According to another preferred embodiment of the present invention, the specific binding substance is adsorbed on the adsorption layer formed on the inner surface of the large number of through holes formed on the substrate and labeled with the labeling substance. The substance derived from the living body is also hybridized with the specific binding substance contained in the adsorption layer. Therefore, when the biochemical analysis unit is washed, it is formed on the inner surface of the through hole. It suffices to wash the adsorption layer, and since the adsorption layer has a large surface area, the biochemical analysis unit can be efficiently washed and reused.

In the present invention, a porous material or a fibrous material is preferably used as the adsorptive material forming the adsorption layer of the biochemical analysis unit. The adsorption layer can also be formed by using a porous material and a fiber material together.

In the present invention, the porous material used for forming the adsorption layer of the biochemical analysis unit may be either an organic material or an inorganic material, or an organic / inorganic composite.

In the present invention, the organic porous material used for forming the adsorption layer of the biochemical analysis unit is
Although not particularly limited, a carbon porous material such as activated carbon or a porous material capable of forming a membrane filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6 and nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid / polylysine polyion complex, etc. Examples thereof include alginic acids; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluorides such as polytetrafluoride, and copolymers or composites thereof.

In the present invention, the inorganic porous material used for forming the adsorption layer of the biochemical analysis unit is
Although not particularly limited, preferably, for example, metals such as platinum, gold, iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metals such as hydroxyapatite and calcium sulfate. Examples thereof include salts and complexes of these.

In the present invention, the fibrous material used for forming the adsorption layer of the biochemical analysis unit is not particularly limited, but preferably, for example, nylon 6, nylon 6,6, nylon. Examples thereof include nylons such as 4,10 and the like, cellulose derivatives such as nitrocellulose, cellulose acetate, and cellulose acetate butyrate.

In the present invention, the adsorption layer is formed by electrolytic treatment, plasma treatment, oxidation treatment such as arc discharge; primer treatment using a silane coupling agent, titanium coupling agent, etc .; surface treatment such as surfactant treatment. You can also do it.

In another preferred embodiment of the present invention, the plurality of adsorption layers are formed by surface-treating the substrate with a surface modifier.

In a preferred aspect of the present invention, the surfaces of the plurality of adsorption layers are roughened.

According to a preferred embodiment of the present invention, since the surface of the adsorption layer is roughened, the adsorption layer has a large adsorption surface area, and therefore a sufficient amount of the specific binding substance is adsorbed on the adsorption layer. It becomes possible to adsorb.

In a further preferred aspect of the present invention, the surface of the adsorption layer is roughened so as to have a fractal structure.

According to a further preferred embodiment of the present invention, the surface of the adsorption layer has a fractal structure,
Being roughened, the adsorption layer has an adsorption surface area 100 times or more larger than that of the case where the surface is smooth, and therefore, it is possible to adsorb a sufficient amount of the specific binding substance to the adsorption layer. It will be possible.

In a preferred embodiment of the present invention, the substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, antigen-antibody reaction, and receptor-ligand.

In a preferred embodiment of the present invention, the radiation energy-attenuating material reduces the energy of the radiation when it penetrates through the material by a distance equal to the distance between adjacent adsorbent layers. , And has the property of being attenuated to 1/5 or less.

[0048] In a further preferred aspect of the present invention, the radiation energy-attenuating material is such that when the radiation penetrates through the material by a distance equal to the distance between adjacent adsorbing layers, the energy of the radiation is reduced. 1
It has the property of being attenuated to / 10 or less.

In a further preferred aspect of the present invention, the radiation energy-attenuating material is such that when the radiation penetrates through the material by a distance equal to the distance between adjacent adsorption layers, the energy of the radiation is reduced. 1
It has the property of being attenuated to / 50 or less.

[0050] In a further preferred aspect of the present invention, the radiation energy attenuating material has a radiation energy when the radiation penetrates through the material by a distance equal to a distance between the adsorbing layers adjacent to each other. 1
It has the property of attenuating to less than / 100.

[0051] In a further preferred aspect of the present invention, the radiation energy-attenuating material is such that when the radiation penetrates through the material by a distance equal to the distance between the adjacent adsorption layers, the energy of the radiation is reduced. 1
It has the property of being attenuated to / 500 or less.

In a further preferred aspect of the present invention, the radiation energy-attenuating material is such that when the radiation penetrates through the material by a distance equal to the distance between adjacent adsorbing layers, the energy of the radiation is reduced. 1
It has the property of being attenuated to less than / 1000.

In a preferred embodiment of the present invention, the light energy attenuating material reduces the energy of the light when the light is transmitted through the material by a distance equal to the distance between the adsorbing layers adjacent to each other. , And has the property of being attenuated to 1/5 or less.

In a further preferred aspect of the present invention, the energy of light is attenuated when the material for attenuating the light energy transmits the material through a distance equal to the distance between the adsorbing layers adjacent to each other. Is attenuated to 1/10 or less.

[0055] In a further preferred aspect of the present invention, the energy of light is attenuated when the light-attenuating material transmits through the material by a distance equal to a distance between the adsorbing layers adjacent to each other. Has the property of being attenuated to 1/50 or less.

[0056] In a further preferred aspect of the present invention, the energy of light is attenuated when the material for attenuating the light energy transmits through the material by a distance equal to the distance between the adjacent adsorption layers. Has the property of being attenuated to 1/100 or less.

[0057] In a further preferred aspect of the present invention, the energy of light is attenuated when the material for attenuating the light energy transmits the material through a distance equal to the distance between the adsorbing layers adjacent to each other. Has a property of attenuating to 1/500 or less.

In a further preferred aspect of the present invention, the light energy attenuating material has a light energy when the light is transmitted through the material by a distance equal to a distance between the adsorbing layers adjacent to each other. Is attenuated to 1/1000 or less.

In the present invention, the material for forming the substrate of the biochemical analysis unit is not particularly limited as long as it has a property of attenuating radiation and / or light energy, and an inorganic compound material,
Any of the organic compound materials can be used,
Metal material, ceramic material or plastic material
Preferably used.

In the present invention, inorganic compound materials which can be preferably used for forming the substrate of the biochemical analysis unit and which can attenuate radiation and / or light energy include, for example, gold, silver, copper, Metals such as zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, silicon nitride, etc. Silicon materials; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material capable of attenuating radiation and / or light energy, and can be preferably used for forming a substrate of a biochemical analysis unit, Examples of the polymer compound capable of attenuating radiation and / or light energy include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride. Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon such as Ron 6, nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenyl siloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer Polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate and hydroxypropylmethylcellulose; chitin; chitosan; sumacum; polyamides such as gelatin, collagen and keratin, and copolymers of these polymer compounds. it can. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, in the present invention, when the substrate of the biochemical analysis unit is made of a material having the property of attenuating the radiation, the specific gravity is 1.0 g. / Cm ³ or more of the compound material or the composite material is preferable, and it is particularly preferable that the specific gravity is 1.5 g / cm ³ or more and 23 g / cm ³ or less of the compound material or the composite material.

Generally, the greater the scattering and / or absorption of light, the higher the light attenuating ability. Therefore, in the present invention, the substrate of the biochemical analysis unit is formed of a material having a property of attenuating light energy. In this case, the absorbance per 1 cm of thickness is preferably 0.3 or more, and more preferably the absorbance per 1 cm of thickness is 1 or more. Here, the absorbance is calculated by calculating the A / T by placing an integrating sphere immediately after the plate having a thickness of Tcm and measuring the transmitted light amount A at the wavelength of the probe light or the emission light used for the measurement with a spectrophotometer. By,
Desired. In the present invention, in order to improve the light attenuating ability, a light scatterer or a light absorber may be contained in the substrate of the biochemical analysis unit. Fine particles of a material different from the material forming the substrate of the biochemical analysis unit are used as the light scatterer, and pigments or dyes are used as the light absorber.

In a preferred embodiment of the present invention, the substrate of the biochemical analysis unit is made of a flexible material.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit is made of a flexible material, the biochemical analysis unit is curved to bring the hybridization reaction solution into contact therewith, Biological substances can be hybridized with specific binding substances, and therefore, it is possible to hybridize specific binding substances with biological substances as desired using a small amount of hybridization reaction solution. become.

In a preferred aspect of the present invention, the plurality of holes are regularly formed in the substrate of the biochemical analysis unit.

[0067] In a preferred aspect of the present invention, the plurality of holes are formed in a substantially circular shape in the substrate of the biochemical analysis unit.

In another preferred aspect of the present invention, the plurality of holes are formed in a substantially rectangular shape in the substrate of the biochemical analysis unit.

In a preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 10 or more holes.

[0070] In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 50 or more holes.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 100 or more holes.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed with 500 or more holes.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
The above holes are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 5000
The above holes are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
Zero or more holes are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 5000
Zero or more holes are formed.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is provided with 1000
00 or more holes are formed.

In a preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 0.1 mm 2.

[0082] In a further preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 0.05 mm 2.

In a further preferred aspect of the present invention, each of the plurality of holes formed in the substrate of the biochemical analysis unit has a size of less than 0.01 mm 2.

In the present invention, the density of the holes formed in the substrate of the biochemical analysis unit is determined by the type of substrate, the type of electron beam emitted from the radiolabeled substance, and the like.

[0085] In a preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 10 holes / cm 2 or more.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 50 holes / cm 2 or more.

In a further preferred aspect of the present invention, the plurality of holes are provided in the substrate of the biochemical analysis unit at a density of 100 holes / cm 2 or more,
Has been formed.

[0088] In a further preferred aspect of the present invention, the plurality of holes are provided in the substrate of the biochemical analysis unit at a density of 500 or more per cm 2.
Has been formed.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 1,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 5000 holes / cm 2 or more.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 10,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 50,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of holes are formed in the substrate of the biochemical analysis unit at a density of 100,000 holes / cm 2 or more.

[0094]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention, and FIG. 2 is a schematic partial sectional view thereof.

As shown in FIG. 1, the biochemical analysis unit 1 according to this embodiment is made of aluminum having a property of attenuating radiation energy and light energy, and a large number of substantially circular recesses 3 are formed in high density. The substrate 2 formed in the above.

As shown in FIG. 2, the adsorption layer 4 is formed on the inner surfaces 3a of the large number of recesses 3 by nitrocellulose capable of forming a membrane filter.

Although not shown exactly in FIG. 1, in this embodiment, the recesses 3 having a size of about 0.01 square millimeters of about 10,000 are regularly arranged at a density of about 5000 pieces / square centimeter. Formed on the substrate 2.

FIG. 3 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 2, a solution containing a specific binding substance, such as a base sequence, is placed in a large number of recesses 3 formed in the substrate 2 of the biochemical analysis unit 1. A known solution containing a plurality of different cDNAs is dropped using the spotting device 5, and the specific binding substance is fixed in the adsorption layer 3 formed in the large number of recesses 3.

As shown in FIG. 3, the spotting device 5 includes an injector 6 for injecting a solution containing a specific binding substance toward the biochemical analysis unit 1 and a CCD.
A camera 7 is provided, and the CCD camera 7 allows the tip of the injector 6 and a specific binding substance such as cDNA
While observing the recessed portion 3 of the biochemical analysis unit 1 to which the solution containing is dropped, when the tip of the injector 6 and the center of the recessed portion 3 to which the solution containing the specific binding substance is dropped, A solution containing a specific binding substance is placed in a large number of recesses 3 of the biochemical analysis unit 1 in which the solution containing the specific binding substance is dropped from the injector 6 and the adsorption layer 4 is formed. Guaranteed to be able to drip accurately.

The specific binding substance dropped in each recess 3 is adsorbed by the adsorption layer 4 formed on the inner surface of each recess.

FIG. 4 is a schematic vertical sectional view of a hybridization reaction container.

As shown in FIG. 4, the hybridization reaction container 8 has a rectangular cross section, and a hybridization reaction solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance, is housed inside. ing.

In the case of selectively labeling a specific binding substance such as cDNA with a radiolabeling substance, a hybridization reaction solution 9 containing a substance derived from a living body which is a probe labeled with the radiolabeling substance is prepared, It is housed in the hybridization reaction container 8.

On the other hand, by using a labeling substance that produces chemiluminescence by contacting with a chemiluminescent substrate, the cDNA is
In the case of selectively labeling a specific binding substance such as, a hybridization reaction solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, It is prepared and housed in the hybridization reaction container 8.

Furthermore, in the case of selectively labeling a specific binding substance such as cDNA with a fluorescent substance such as a fluorescent dye, a high-molecular substance containing a probe which is a probe labeled with a fluorescent substance such as a fluorescent dye is used. A hybridization reaction solution 9 is prepared and housed in the hybridization reaction container 8.

[0108] A substance derived from a living body labeled with a radioactive labeling substance, a substance derived from a living body labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate, and a living body labeled with a fluorescent substance such as a fluorescent dye It is also possible to prepare a hybridization reaction solution 9 containing two or more substances derived from a living body among the substances derived from the living body and store it in the hybridization reaction container 8. In this embodiment, the hybridization reaction solution 9 is labeled with a radioactive labeling substance. Hybridization reaction solution containing a biologically-derived substance, a biologically-derived substance labeled with a fluorescent substance such as a fluorescent dye, and a biologically-derived substance labeled with a labeling substance that causes chemiluminescence by contact with a chemiluminescent substrate 9 was prepared and placed in the hybridization reaction container 8. It is content.

Upon hybridization, cD
A biochemical analysis unit 1 in which a specific binding substance such as NA is adsorbed on an adsorption layer 4 formed on the inner surfaces of a large number of recesses 3 formed on a substrate 2 is inserted into a hybridization container 8. It

As a result, the specific binding substance adsorbed on the adsorption layer 4 formed on the inner surface of the large number of recesses 3 was labeled with the radioactive labeling substance and was derived from the living body contained in the hybridization reaction solution 9. Substance, a fluorescent substance such as a fluorescent dye, and a labeling substance that causes chemiluminescence when brought into contact with the substance of biological origin contained in the hybridization reaction solution 9 and the chemiluminescent substrate, and the hybridization reaction solution 9
The substance of biological origin contained in is selectively hybridized.

In this embodiment, the specific binding substance that is a probe is adsorbed in the adsorption layer 4 formed on the inner surface 3a of each recess 3, and the recess 3 is filled with a porous material. As compared with the case where the adsorption region is formed, the adsorption reaction is performed in a region having a small volume, so that the reaction rate of hybridization can be improved, and the hybridization reaction solution 9 can be formed in a sufficiently large area. Since it can be brought into contact with the adsorption layer 4, the probability that the substance of biological origin that is the target contained in the hybridization solution 9 will encounter the specific binding substance that is the probe adsorbed in the adsorption layer 4 is increased. Therefore, the efficiency of hybridization can be significantly improved.

In this way, the adsorption layer 4 formed on the inner surface of the large number of recesses 3 of the biochemical analysis unit 1 is brought into contact with the radiation data of the radioactive labeling substance, the fluorescence data of the fluorescent substance such as a fluorescent dye, and the chemiluminescent substrate. The chemiluminescence data of the labeling substance that causes chemiluminescence by the recording is recorded.

A large number of adsorption layers 4 of the biochemical analysis unit 1
The fluorescence data recorded in (1) is read by a scanner described later to generate biochemical analysis data.

Further, the chemiluminescence data recorded in the numerous absorptive regions 4 of the biochemical analysis unit 1 is read by a cooling CCD camera of a data generating system described later or transferred to a stimulable phosphor sheet. Then, it is read by a scanner described later, and biochemical analysis data is generated.

On the other hand, the radiation data recorded on the large number of adsorption layers 4 of the biochemical analysis unit 1 are transferred to the large number of stimulable phosphor layer regions of the stimulable phosphor sheet and read by a scanner described later. Then, biochemical analysis data is generated.

FIG. 5 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 5, the stimulable phosphor sheet 10 has a support 11, and one surface of the support 11 has a large number of recesses 3 formed in the biochemical analysis unit 1.
A large number of concave portions 13 are formed in the same pattern as the above pattern, and the stimulable phosphor is embedded in the large number of concave portions 13 to form a large number of substantially circular stimulable phosphor layer regions 12. There is.

In this embodiment, the support 11 is made of stainless steel having a property of attenuating radiation, and the large number of recesses 13, and therefore the substantially circular stimulable phosphor layer region 12, are biochemically analyzed. Board 2 of unit 1
It is formed in the support 11 so as to have the same diameter as the recess 3 formed in the.

FIG. 6 shows that the radioactive labeling substance contained in the adsorption layer formed on the inner surface of a large number of recesses formed on the substrate of the biochemical analysis unit is formed on the support of the stimulable phosphor sheet. It is a schematic sectional drawing which shows the method of exposing many photostimulable fluorescent substance layer area | regions.

As shown in FIG. 6, upon exposure,
Each of the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 has a biochemical analysis unit 1
The stimulable phosphor sheet 10 is placed in the biochemical analysis unit 1 so as to face the corresponding recessed portions 3 formed on the substrate 2.
Overlaid on top.

In this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum, it hardly expands or contracts even when subjected to liquid treatment such as hybridization.
Each of the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is accurately formed in the recess 3 of the corresponding biochemical analysis unit 1 having the adsorption layer 4 formed on the inner surface thereof. The stimulable phosphor layer region 12 can be exposed by easily and surely superimposing the stimulable phosphor sheet 10 and the biochemical analysis unit 1 so as to face each other.

Thus, over a predetermined time, each of the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the corresponding raw material having the adsorption layer 4 formed on the inner surface thereof. A large number of stimulable phosphor layer regions formed on the support 11 of the stimulable phosphor sheet 10 by the radioactive labeling substance contained in the adsorption layer 4 by closely contacting with the recess 3 of the chemical analysis unit 1. 12 is exposed.

At this time, an electron beam (β-ray) is emitted from the radiolabeled substance contained in the many adsorption layers 4 of the biochemical analysis unit 1.
However, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating radiation energy, the biochemical analysis unit 1
The electron beam (β-ray) emitted from the radiolabeled substance contained in the adsorption layer 4 is reliably prevented from being scattered in the substrate 2 of the biochemical analysis unit 1, and the stimulable phosphor is also present. Since the support 11 of the sheet 10 is formed of stainless steel having a property of attenuating the radiation energy, an electron beam emitted from the radiolabel substance contained in the adsorption layer 4 of the biochemical analysis unit 1 ( β ray)
Scattering within the support 11 of the stimulable phosphor sheet 10 is prevented, so that the electron beam (β-ray) emitted from the radiolabeled substance contained in the adsorption layer 4 of the biochemical analysis unit 1 is Therefore, it is possible to reliably prevent the photostimulable phosphor layer region 12 facing the recess 3 adjacent to the recess 4 in which the adsorption layer 4 is formed.

Therefore, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10.
Can be reliably exposed only by the radioactive labeling substance contained in the adsorption layer 4 formed on the inner surface 3a of the corresponding recess 3 of the biochemical analysis unit 1.

Thus, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are
Radiation data of radiolabeled substances are recorded.

FIG. 7 shows the radiation data of the radiolabeled substance recorded in a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the substrate of the biochemical analysis unit 1. 2 is a schematic perspective view showing an example of a scanner that reads fluorescence data of a fluorescent substance such as a fluorescent dye recorded in an adsorption layer 4 in a large number of recesses 3 formed in 2 to generate biochemical analysis data, FIG. 8 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner shown in FIG. 7 is used for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. It is configured to be able to read fluorescence data of a fluorescent substance such as a fluorescent dye recorded in an adsorption layer 4 in a large number of recesses 3 formed in the substrate 2 of the unit 1, and emit a laser beam 24 having a wavelength of 640 nm. 1 laser excitation light source 21 and laser light 24 having a wavelength of 532 nm
And a third laser excitation light source 23 which emits a laser beam 24 having a wavelength of 473 nm.

In this embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source,
Laser excitation light source 22 and third laser excitation light source 23
Is composed of a second harmonic generation element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. The first dichroic mirror 27 and 532 nm that transmit the laser light 4 of 640 nm and reflect the light of wavelength 532 nm are transmitted through the optical path of the laser light 24 emitted from the first laser excitation light source 21 and reflected by the mirror 26. A second dichroic mirror 28 that transmits light of the above wavelength and reflects light of the wavelength of 473 nm is provided, and the laser light 24 generated by the first laser excitation light source 21 is provided.
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated from the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, the first dichroic mirror 27
Is reflected by the second dichroic mirror 28, the direction of which is changed by 90 degrees, the light is transmitted through the second dichroic mirror 28, and is incident on the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

Laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser light 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 or the biochemical analysis unit 1 placed on 41. In FIG. 7, the biochemical analysis unit 1 is placed on the glass plate 41 of the stage 40 such that the surface on which the recess 3 is formed faces downward.

When the laser beam 24 enters the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10, the laser beam 24 is included in the stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10. When the stimulable phosphor that has been excited is excited to emit stimulable light 45 and the laser light 24 is incident on the adsorption layer 4 of the biochemical analysis unit 1, the adsorption formed on the inner surface 3a of the recess 3 is detected. The fluorescent dye contained in the layer 4 is excited and the fluorescence 45 is emitted.

Photostimulated light 45 emitted from the photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 or formed on the inner surface 3a of the recess 3 formed in the substrate 2 of the biochemical analysis unit 1. The fluorescence 45 emitted from the adsorption layer 4 is
The aspherical lens 37 provided in the optical head 35 collects the light on a mirror 36, and the mirror 36 reflects the laser light 24 on the same side as the optical path of the laser light 24 to form parallel light, which is then incident on a concave mirror 38.

The photostimulable light 45 or the fluorescent light 45 incident on the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or the fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror to enter the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 8, the filter unit 48 includes four filter members 51a, 51b, 51c,
51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 8 by a motor (not shown).

FIG. 9 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 9, the filter member 51
a includes a filter 52a, and the filter 52a uses the first laser excitation light source 21 and uses the biochemical analysis unit 1
The fluorescent substance such as a fluorescent dye contained in the adsorption layer 4 formed on the inner surface 3a of the large number of the concave portions 3 is excited to generate fluorescence 45
64 is a filter member used when reading
It has a property of cutting light with a wavelength of 0 nm and transmitting light with a wavelength longer than 640 nm.

FIG. 10 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 10, the filter member 5
1b includes a filter 52b, and the filter 52b includes a second
The laser excitation light source 22 is used to excite a fluorescent substance such as a fluorescent dye contained in the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the biochemical analysis unit 1 to generate fluorescence 4
5 is a filter member used when reading 5.
It has a property of cutting light having a wavelength of 32 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 11 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 11, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
The laser excitation light source 23 is used to excite a fluorescent substance such as a fluorescent dye contained in the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the biochemical analysis unit 1 to generate fluorescence 45. It is a filter member used when reading,
It has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 12 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 12, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
No. 45 of the stimulable phosphor layer region 12 formed on the support 11 of 0. A stimulable phosphor layer region 1 which is a filter used when reading
2 has a property of transmitting only the light in the wavelength region of the stimulated emission light 45 emitted from No. 2 and cutting the light of the wavelength of 640 nm.

Therefore, depending on the laser excitation light source to be used, the filter members 51a, 51b, 51c, 51d.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated is converted into digital data by the A / D converter 53 and sent to the data processor 54.

Although not shown in FIG. 7, the optical head 35 is moved by the scanning mechanism in the sub-scanning direction orthogonal to the main scanning direction indicated by arrow X and the main scanning direction indicated by arrow Y in FIG. All the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 or all the adsorption layers 4 formed on the substrate 2 of the biochemical analysis unit 1 are configured to be movable. The laser beam 24 is used for scanning.

FIG. 13 is a schematic plan view of the scanning mechanism of the optical head.

In FIG. 13, for simplification, the optical system excluding the optical head 35 and the optical paths of the laser light 24 and the stimulable light 45 or the fluorescent light 45 are omitted.

As shown in FIG. 13, the optical head 35.
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
And 6 are fixed on the substrate 60 and are movable in the sub-scanning direction indicated by arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in this hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63, and the main scanning stepping motor 65 places the endless belt 66 at the distance between the adjacent recesses 3 formed in the biochemical analysis unit 1. It can be driven intermittently at equal pitches.

The optical head 35 includes an endless belt 66.
When the endless belt 66 is driven by the main scanning stepping motor 65, the endless belt 66 is moved in the main scanning direction indicated by the arrow X in FIG.

In FIG. 13, 67 is the optical head 35.
Is a linear encoder for detecting the position in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is driven in the main scanning direction, and the sub-scanning pulse motor 61 drives the substrate 63.
By being intermittently moved in the sub-scanning direction, the optical head 35 is moved in the main-scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG. All stimulable phosphor layer regions 1 formed on the support 11 of the stimulable phosphor sheet 10 by
2 or all adsorption layers 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned.

FIG. 14 is a block diagram showing the control system, input system, drive system and detection system of the scanner shown in FIG.

As shown in FIG. 14, the scanner control system includes a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 which is operated by the user and can input various instruction signals.

As shown in FIG. 14, the scanner drive system intermittently moves the optical head 35 in the main scanning direction and the main scanning stepping motor 65, and intermittently moves the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

Further, as shown in FIG. 14, the detection system of the scanner is provided with a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser pumping light source 21, the second laser pumping light source 22 or the third laser pumping light source 21 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The scanner constructed as described above is arranged as described below, so that the radioactive layer contained in the adsorption layer 4 formed on the inner surface 3a of the large number of recesses 3 formed in the biochemical analysis unit 1 will be described. A large number of stimulable phosphor layer regions 12 are exposed by the labeling substance, and the radiation data of the radioactive labeling substance recorded in the large number of stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 is read, Generates data for biochemical analysis.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40.

Next, the keyboard 7 is selected by the user.
An instruction signal for scanning a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 with the laser beam 24 is input to the first column.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
In the optical path of the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 by providing a filter member 51d having a filter 52d having a property of transmitting only light in the wavelength range of Located in.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, Of the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10, the first stimulable phosphor layer region 12 is located at a position where the laser light 24 can be irradiated.
When it is confirmed that the optical head 35 has reached, a stop signal is output to the main scanning stepping motor 65, and
The drive signal is output to the first laser excitation light source 21 to
The laser excitation light source 21 is activated to emit the laser light 24 having a wavelength of 640 nm.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 on the first stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41.

In this embodiment, each of the photostimulable phosphor layer regions 12 has a recess 1 formed in a stainless steel support 11 having a property of attenuating light energy.
Since the stimulable phosphor layer is formed by being embedded in 3, the laser beam 24 is scattered in each stimulable phosphor layer area 12 and adjacent stimulable phosphor layer areas are formed. It becomes possible to effectively prevent the stimulable phosphor contained in the adjacent stimulable phosphor layer regions 12 from being excited into the inside of 12 and being excited.

The laser light 24 causes the stimulable phosphor sheet 10 to emit light.
First stimulable phosphor layer region 1 formed on the support 11 of
When incident on 2, the stimulable phosphor contained in the first stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser light 24 to generate the first stimulable phosphor. The photostimulable light 45 is emitted from the stimulable phosphor layer region 12.

The photostimulable light 45 emitted from the first photostimulable phosphor region 12 is condensed by the aspherical lens 37 provided in the optical head 35, and the same as the optical path of the laser light 24 by the mirror 36. Is reflected to the side and made into parallel light,
It is incident on the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 34 is reflected by the concave mirror 38.
Incident on.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and is incident on the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the stimulable light 45 emitted from the stimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected by the photomultiplier 50 and generated is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

After the first laser excitation light source 21 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21, The drive of the first laser excitation light source 21 is stopped, and a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move to the adjacent luminescent elements formed on the support 11 of the stimulable phosphor sheet 10. A pitch equal to the distance between the exhaustive phosphor layer regions 12,
To move.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is confirmed to have been moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 12, the control unit 70 outputs a drive signal to the first laser excitation light source 21. The stimulable phosphor sheet 10 is turned on by turning on the first laser excitation light source 21 and the laser light 24.
First stimulable phosphor layer region 1 formed on the support 11 of
The stimulable phosphor contained in the second stimulable phosphor layer region 12 adjacent to 2 is excited.

Similarly, the laser light 24 is irradiated for a predetermined time on the second stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10, and the second stimulable phosphor layer region 12 is irradiated.
Photostimulable light 45 emitted from the photostimulable phosphor layer region 12 of
However, when it is detected photoelectrically by the photomultiplier 50, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21 to turn off the first laser excitation light source 21, and A drive signal is output to the scanning stepping motor 65 to output the optical head 35.
Are moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 12.

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 inputted from the linear encoder 67, Optical head 35
Is moved by one line in the main scanning direction, and the scanning of the stimulable phosphor layer region 12 of the first line formed on the support 11 of the stimulable phosphor sheet 10 by the laser light 24 is completed. When it is confirmed, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position and outputs a drive signal to the sub scanning pulse motor 61. , The movable substrate 63 in the sub-scanning direction by one line,
To move.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the stimulable phosphor layer of the first line formed on the support 11 of the stimulable phosphor sheet 10 to be formed. The region 12 is sequentially irradiated with the laser beam 24 emitted from the first laser excitation light source 21, and in the same manner as in the case where the region 12 is sequentially irradiated with the laser beam 24 of the second line formed on the support 11 of the stimulable phosphor sheet 10. Of the stimulable phosphor layer region 1 on the second line by sequentially irradiating the stimulable phosphor layer region 12 with laser light 24 emitted from the first laser excitation light source 21.
The stimulable phosphor contained in No. 2 is excited, and the stimulable light 45 emitted from the stimulable phosphor layer region 12 is sequentially detected photoelectrically by the photomultiplier 50.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

Thus, all the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are
The photostimulable phosphor layer region 1 is scanned by the laser beam 24.
The stimulable phosphor contained in 2 is excited, and the emitted stimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is A / D.
When converted into digital data by the converter 53 and sent to the data processing device 54, a drive stop signal is sent from the control unit 70 to the first laser excitation light source 21.
Is output, and the driving of the first laser excitation light source 21 is stopped.

Thus, the radiation data recorded in the large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is read, and biochemical analysis data is generated.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the adsorption layer 4 formed on the inner surfaces 3a of the large number of recesses 3 of the biochemical analysis unit 1 is read to generate digital data for biochemical analysis. First, the user sets the biochemical analysis unit 1 on the glass plate 41 of the stage 40.

Next, the user operates the keyboard 7
A fluorescent substance identification signal that identifies the type of fluorescent dye that is a labeling substance and an instruction signal that the fluorescence data should be read are input to 1.

The fluorescent substance specifying signal and the instruction signal input to the keyboard 71 are input to the control unit 70, and when the control unit 70 receives the fluorescent substance specifying signal and the instruction signal, the control unit 70 stores them in a memory (not shown). The laser excitation light source to be used is determined according to the table shown in FIG.
Which of b and 52c is to be positioned in the optical path of the fluorescent light 45 is determined.

For example, Rhodamine (registered trademark), which can be most efficiently excited by a laser having a wavelength of 532 nm, as a fluorescent substance for labeling a substance of biological origin
Is used and is input to the keyboard 71, the control unit 70 selects the second laser excitation light source 22, selects the filter 52b, and outputs a drive signal to the filter unit motor 72. Then, the filter unit 48 is moved to cut the light having the wavelength of 532 nm, and the filter member 51b having the property of transmitting the light having the wavelength longer than 532 nm is emitted from the biochemical analysis unit 1. It is located in the optical path of the fluorescent light 45 to be formed.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, Of the large number of concave portions 3 formed on the substrate 2 of the biochemical analysis unit 1, the adsorption layer 4 formed on the inner surface 3a of the first concave portion 3 can be irradiated with the laser beam 24 at a position where the optical head can be irradiated. When it is confirmed that 35 has been reached, a stop signal is output to the main scanning stepping motor 65 and
A drive signal is output to the laser excitation light source 22 to activate the second laser excitation light source 22 to emit the laser light 24 having a wavelength of 532 nm.

The laser light 24 emitted from the second laser excitation light source 22 is made into parallel light by the collimator lens 30, and then enters the first dichroic mirror 27 and is reflected.

The laser beam 24 reflected by the first dichroic mirror 27 passes through the second dichroic mirror 28 and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and focused by the aspherical lens 37 on the adsorption layer 4 formed in the first recess 4 of the biochemical analysis unit 1 mounted on the stage 40 glass plate 41. .

In this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having the property of attenuating the light energy, the laser light 24 emits the laser beam 24 to the substrate 2 of the biochemical analysis unit 1. It is possible to effectively prevent the light from being scattered inside and entering the adjacent concave portions 4.

The laser beam 24 is emitted from the biochemical analysis unit 1
When incident on the adsorption layer 4 formed in the first concave portion 4 of
Rhodamine contained in the adsorption layer 4 formed in the first recess 4 of the biochemical analysis unit 1 is excited by the laser beam 24, and fluorescence 45 is emitted from the rhodamine.

In the biochemical analysis unit 1 according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating light energy, a fluorescent substance is used. It is possible to reliably prevent the fluorescence emitted from the substrate from being scattered in the substrate 2 and mixed with the fluorescence emitted from the fluorescent substance contained in the adsorption layer 4 formed on the inner surfaces of the adjacent recesses 3. it can.

The fluorescent light 45 emitted from the rhodamine is collected by the aspherical lens 37 provided in the optical head 35, reflected by the mirror 36 to the same side as the optical path of the laser light 24, and made into parallel light. , Enters the concave mirror 38.

The fluorescent light 45 which has entered the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.

The fluorescence 45 that has entered the perforated mirror 34 is
The perforated mirror 34 formed by the concave mirror reflects the light downward as shown in FIG. 8 and makes it incident on the filter 52b of the filter unit 48.

Since the filter 52b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm which is excitation light is cut and emitted from rhodamine. Fluorescence 45
Only the light in the wavelength range of 1 passes through the filter 52b and is photoelectrically detected by the photomultiplier 50.

The analog data photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53 and converted into digital data.
It is output to the data processing device 54.

When a predetermined time, for example, several μ seconds has elapsed after the second laser excitation light source 22 was turned on, the control unit 70 outputs a drive stop signal to the second laser excitation light source 22, The drive of the second laser excitation light source 22 is stopped, and a drive signal is output to the main scanning stepping motor 65 so that the optical head 35 is recessed in the biochemical analysis unit 1 adjacent to each other in the main scanning direction. Move by a pitch equal to the distance between three.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the recesses 3 adjacent to each other in the main scanning direction of the biochemical analysis unit 1, and the laser beam 24 emitted from the second laser excitation light source 22 is emitted from the biochemical analysis unit 1 When it is confirmed that the adsorption layer 4 formed on the inner surface 3a of the second concave portion 3 has moved to a position where it can be irradiated, the control unit 70 outputs a drive stop signal to the main scanning stepping motor 65 and , Outputs a drive signal to the second laser excitation light source 22,
The second laser excitation light source 22 is turned on, and the laser light 24
Thus, the fluorescent substance contained in the adsorption layer 4 formed on the inner surface 3a of the second recess 3 of the biochemical analysis unit 1, for example, rhodamine is excited.

Similarly, the laser beam 24 is applied to the adsorption layer 4 formed on the inner surface 3a of the second concave portion 3 of the biochemical analysis unit 1 for a predetermined time, and the laser light 24 is emitted from the second concave portion 3. When the fluorescence 45 emitted from the adsorption layer 4 formed on the inner surface 3a is photoelectrically detected by the photomultiplier 50 and analog data is generated, the control unit 70 causes the second laser excitation light source. A drive stop signal is output to 22 to turn off the second laser excitation light source 22, and to the main scanning stepping motor 65,
A drive signal is output to move the optical head 35 by one pitch equal to the distance between the adjacent recesses 3 of the biochemical analysis unit 1.

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 input from the linear encoder 67, Optical head 35
Is moved by one line in the main scanning direction, and the adsorption layer 4 formed on the inner surfaces 3a of all the recesses 3 of the first line formed on the substrate 2 of the biochemical analysis unit 1 When the scanning is confirmed by the light 24, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position and drive the sub scanning pulse motor 61. A signal is output to move the movable substrate 63 by one line in the sub-scanning direction.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the adsorption layer 4 formed on the inner surface 3a of the recess 3 of the first line of the biochemical analysis unit 1 to be formed. Then, the adsorption formed on the inner surface 3a of the concave portion 3 of the second line of the biochemical analysis unit 1 is performed in the same manner as the laser light 24 emitted from the second laser excitation light source 22 is sequentially irradiated. The layer 4 is sequentially irradiated with the laser light 24 emitted from the second laser excitation light source 22 to excite the rhodamine contained in the adsorption layer 4 formed on the inner surface 3a of the recess 3 to generate the adsorption layer 4 The fluorescence 45 emitted from the
It is detected photoelectrically.

The photomultiplier 50 photoelectrically detects the fluorescence 45 and the generated analog data is converted into digital data by the A / D converter 53 and sent to the data processing device 54.

In this way, all the adsorption layers 4 of the biochemical analysis unit 1 are scanned by the laser light 24 emitted from the second laser excitation light source 22, and the adsorption formed on the inner surfaces 3a of the large number of recesses 3 is detected. Rhodamine contained in the layer 4 is excited, and the emitted fluorescence 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into digital data by the A / D converter 53. Then, when it is sent to the data processing device 54, a drive stop signal is sent from the control unit 70.
It is output to the second laser excitation light source 22, and the driving of the second laser excitation light source 22 is stopped.

In this way, the fluorescence data recorded on the inner surface 3a of the large number of recesses 3 formed in the substrate 2 of the biochemical analysis unit 1 is read, and the biochemical analysis data is generated.

FIG. 15 shows the chemistry of a labeling substance that causes chemiluminescence by bringing it into contact with a chemiluminescent substrate recorded in the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the biochemical analysis unit 1. It is a schematic front view of the data generation system which reads luminescence data and produces | generates the data for biochemical analysis.

The data generation system shown in FIG.
Fluorescence data of a fluorescent substance such as a fluorescent dye recorded in the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the biochemical analysis unit 1 can also be generated.

As shown in FIG. 15, the data generation system comprises a cooled CCD camera 81, a dark box 82 and a personal computer 83. The personal computer 83 includes a CRT 84 and a keyboard 85.

FIG. 16 shows the cooling CC of the data generation system.
4 is a schematic vertical sectional view of a D camera 81. FIG.

As shown in FIG. 16, the cooled CCD camera 81 has a CCD 86, a heat transfer plate 87 made of a metal such as aluminum, a Peltier element 88 for cooling the CCD 86, and a front surface of the CCD 86. Shutter 89, A / D converter 90 for converting analog data generated by CCD 86 into digital data, and A / D converter 90
A data buffer 91 for temporarily storing the data digitized by the / D converter 90 and a camera control circuit 92 for controlling the operation of the cooled CCD camera 81 are provided. An opening formed between the dark box 82 and the dark box 82 is closed by a glass plate 95, and a radiation fin 96 for radiating heat generated by the Peltier element 88 is provided around the cooling CCD camera 81 in the longitudinal direction. It is formed over the entire surface.

A camera lens 97 having a lens focus adjusting function is mounted in the dark box 82 in front of the glass plate 95.

FIG. 17 shows a dark box 82 of the data generation system.
FIG.

As shown in FIG. 17, an LED light source 100 that emits excitation light is provided in the dark box 82, and the LED light source 100 is provided with a filter 101 that is detachably provided and an upper surface of the filter 101. The diffuser plate 103 provided is provided, and the excitation light passes through the diffuser plate 103.
Biochemical analysis unit mounted on it (not shown)
By irradiating the biochemical analysis unit, the biochemical analysis unit is guaranteed to be uniformly irradiated. The filter 101 has a property of cutting light harmful to the excitation of the fluorescent substance other than the wavelength near the excitation light and transmitting only the light of the wavelength near the excitation light. On the front surface of the camera lens 97, a filter 102 that cuts light having a wavelength near the excitation light is provided.
Is detachably provided.

FIG. 18 is a block diagram of the periphery of the personal computer 83 of the data generating system.

As shown in FIG. 18, the personal computer 83 has a CPU 110 for controlling the exposure of the cooled CCD camera 81, a data transfer means 111 for reading out the digital data generated by the cooled CCD camera 81 from the data buffer 91, and a digital signal. Based on the data storage means 112 for storing data, the data processing means 113 for performing data processing on the digital data stored in the data storage means 112, and the digital data stored in the data storage means 112, on the screen of the CRT 84. The data display means 114 which displays visible data is provided. LE
The D light source 100 is controlled by the light source control unit 115, and an instruction signal is input to the light source control unit 115 from the keyboard 85 via the CPU 110. The CPU 110 is configured to be able to output various signals to the camera control circuit 92 of the cooled CCD camera 81.

The data generation system shown in FIGS. 15 to 18 includes a chemiluminescent substrate and a labeling substance contained in the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the biochemical analysis unit 1. Chemiluminescence generated by contact with
C of the cooled CCD camera 81 via the camera lens 97
While detecting by the CD86 and reading chemiluminescence data to generate biochemical analysis data, the biochemical analysis unit 1 is irradiated with excitation light from the LED light source 100 to generate a large number of biochemical analysis units 1. A fluorescent substance such as a fluorescent dye contained in the adsorption layer 4 formed on the inner surface 3a of the recess 3 is excited, and the emitted fluorescence is detected by the CCD 66 of the cooling CCD camera 81 via the camera lens 97. , Is capable of reading fluorescence data and generating biochemical analysis data.

When the chemiluminescence data is read and the biochemical analysis data is generated, the filter 102 is removed, the LED light source 100 is held in the OFF state, and the diffusion plate 1 is removed.
03, the chemiluminescent substrate is brought into contact with the labeling substance contained in the adsorption layer 4 formed on the inner surfaces 3a of the large number of recesses 3 of the biochemical analysis unit 1 to emit chemiluminescence. The unit 1 is placed.

Then, the camera lens 9 is set by the user.
The lens focus is adjusted using 7 and the dark box 8
2 is closed.

After that, when the user inputs an exposure start signal to the keyboard 85, the exposure start signal changes to the CPU 11
0 through the camera control circuit 9 of the cooled CCD camera 81
2 is input, the camera control circuit 92 opens the shutter 89, and the exposure of the CCD 86 is started.

A large number of adsorption layers 4 of the biochemical analysis unit 1
The chemiluminescence emitted from the light enters the photocathode of the CCD 86 of the cooled CCD camera 81 through the camera lens 97 and forms an image on the photocathode. The CCD 86 thus receives the light of the image formed on the photocathode and stores it in the form of charges.

The chemiluminescence emitted from the biochemical analysis unit 1 enters the photoelectric surface of the CCD 86 of the cooled CCD camera 81 through the camera lens 97 and forms an image on the photoelectric surface. The CCD 86 thus receives the light of the image formed on the photocathode and stores it in the form of charges.

Here, in this embodiment, the substrate 2 of the biochemical analysis unit 1 is formed of aluminum having a property of attenuating light energy, and therefore the labeling substance contained in each adsorption layer 4 is used. It is ensured that the chemiluminescence emitted from the substrate is scattered within the substrate 2 and mixes with the chemiluminescence emitted from the labeling substance contained in the adsorption layer 4 formed on the inner surface 3a of the adjacent recesses 3. Can be prevented.

When the predetermined exposure time elapses, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 110, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charge to the A / D converter 100, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 11 to operate.
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When the user inputs a data display signal to the keyboard 85, the CPU 110 causes the digital data stored in the data storage means 112 to be output to the data processing means 113, and the data processing is performed according to the user's instruction. After that, a data display signal is output to the data display means 114, and the biochemical analysis data is displayed on the CRT 8 based on the processed digital data.
Display on the screen of 4.

On the other hand, by reading the fluorescence data,
When generating the biochemical analysis data, first, the biochemical analysis unit 1 is placed on the diffusion plate 103.

Then, the LED light source 10 is selected by the user.
0 is turned on, the lens focus is adjusted using the camera lens 97, and the dark box 82 is closed.

After that, when the user inputs an exposure start signal to the keyboard 85, the light source control means 115 causes
The LED light source 100 is turned on, and excitation light is emitted toward the biochemical analysis unit 1.

Then, when the user inputs an exposure start signal to the keyboard 85, the light source control means 115 causes
The LED light source 100 is turned on, and excitation light is emitted toward the biochemical analysis unit 1.

At the same time, the exposure start signal is input to the camera control circuit 92 of the cooled CCD camera 81 via the CPU 110, and the camera control circuit 92 causes the shutter 89 to be released.
Is opened and the exposure of the CCD 86 is started.

The excitation light emitted from the LED light source 100 is filtered by the filter 101 to eliminate wavelength components other than the excitation light, and is made uniform by the diffusion plate 23, and is irradiated onto the biochemical analysis unit 1. It

When the biochemical analysis unit 1 is irradiated with the excitation light, the fluorescent substance contained in the adsorption layer 4 formed on the inner surfaces 3a of the large number of recesses 3 of the biochemical analysis unit 1, When excited by the excitation light, a large number of adsorption layers 4 of the biochemical analysis unit 1 emit fluorescence.

Many adsorption layers 4 of the biochemical analysis unit 1
The fluorescence emitted from the CCD is passed through the filter 102 and the camera lens 97, and then the CCD 86 of the cooled CCD camera 81.
Incident on the photocathode and forms an image on the photocathode. CCD86
Thus receives the light of the image formed on the photocathode and accumulates it in the form of charges. Since the light having the wavelength of the excitation light is cut off by the filter 102, the fluorescence emitted from the fluorescent substance contained in the adsorption layer 4 formed on the inner surfaces 3a of the large number of recesses 3 of the biochemical analysis unit 1. But only
The light is received by the CCD 86.

Here, in the present embodiment, the substrate 2 of the biochemical analysis unit 1 is formed of aluminum having a property of attenuating light energy, so that fluorescence emitted from a fluorescent substance such as a fluorescent dye is used. However, it can be reliably prevented from being scattered in the substrate 2 and mixed with the fluorescence emitted from the fluorescent substance contained in the adsorption layer 4 formed on the inner surfaces 3 a of the adjacent recesses 3.

When the predetermined exposure time elapses, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 40, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charge to the A / D converter 10, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 21
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When a data display signal is input to the keyboard 85 by the user, the CPU 110 causes the data processing means 113 to output the digital data stored in the data storage means 112, and the data processing is performed according to the user's instruction. After that, a data display signal is output to the data display means 114, and the biochemical analysis data is displayed on the CRT 8 based on the processed digital data.
Display on the screen of 4.

In this embodiment, the chemiluminescence data recorded in the large number of adsorption layers 4 of the biochemical analysis unit 1 is transferred to the stimulable phosphor sheet, and the chemiluminescence transferred to the stimulable phosphor sheet is transferred. The data can be read by a scanner described below to generate biochemical analysis data.

FIG. 19 is a schematic perspective view of a stimulable phosphor sheet to which the chemiluminescence data recorded in the large number of adsorption layers 4 of the biochemical analysis unit 1 should be transferred.

The stimulable phosphor sheet 15 shown in FIG.
Is embedded in a large number of recesses 13 formed in the support 11 with SrS-based stimulable phosphors capable of absorbing and accumulating light energy to form a large number of stimulable phosphor layer regions 17. The stimulable phosphor sheet 10 has the same structure as that of the stimulable phosphor sheet 10 shown in FIG.

The chemiluminescence data recorded in the large number of adsorption layers 4 formed in the biochemical analysis unit 1 shows the large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 shown in FIG. Is transcribed to.

When the chemiluminescence data recorded in the large number of adsorption layers 4 formed in the biochemical analysis unit 1 is transferred to the large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15, The chemiluminescent substrate is brought into contact with the large number of adsorption layers 4 of the biochemical analysis unit 1.

As a result, chemiluminescence in the visible light wavelength range is selectively released from the large number of adsorption layers 4 of the biochemical analysis unit 1.

Then, in the same manner as shown in FIG.
A large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 are used as the biochemical analysis unit 1
So as to face the corresponding large number of concave portions 3 formed in
The stimulable phosphor sheet 15 is superposed on the biochemical analysis unit 1 in which chemiluminescence is emitted from a large number of adsorptive regions 4.

Thus, over a predetermined time, each of the many stimulable phosphor layer regions 17 formed on the stimulable phosphor sheet 15 and the many adsorption layers 4 formed on the biochemical analysis unit 1. Facing each other, a large number of stimulable phosphor layer regions 17 formed on the stimulable phosphor sheet 15 by chemiluminescence selectively emitted from the large number of adsorption layers 4 of the biochemical analysis unit 1. Is exposed.

In this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating light energy, it is selectively selected from the large number of adsorption layers 4 of the biochemical analysis unit 1. The chemiluminescence emitted to the substrate is reliably prevented from being scattered in the substrate 2 of the biochemical analysis unit 1, and the support 11 of the stimulable phosphor sheet 15 has a property of attenuating light energy. Since it is formed of stainless steel,
The chemiluminescence emitted from the adsorption layer 4 of the biochemical analysis unit 1 is prevented from being scattered within the support 11 of the stimulable phosphor sheet 15, and therefore from the adsorption layer 4 of the biochemical analysis unit 1. The emitted chemiluminescence is reliably prevented from reaching the stimulable phosphor layer region 17 facing the recess 3 adjacent to the recess 4 in which the adsorption layer 4 is formed.

Therefore, a large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15.
Can be reliably exposed only by the chemiluminescence emitted from the adsorption layer 4 formed on the inner surface 3a of the corresponding recess 3 of the biochemical analysis unit 1.

In this way, a large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 are
The chemiluminescence data is transcribed and recorded.

FIG. 20 shows that the chemiluminescence data recorded in many stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 is read to generate biochemical analysis data. It is a schematic perspective view of a scanner. Figure 21
FIG. 22 is a schematic perspective view showing details of the scanner in the vicinity of the photomultiplier, and FIG. 22 is a schematic sectional view taken along the line EE of FIG.

In the scanner shown in FIGS. 20 to 22, the third laser excitation light source 23 which emits the laser light 24 having a wavelength of 473 nm is replaced with 980 nm which can efficiently excite the SrS stimulable phosphor. To a filter member 51c having a filter 52c having a property of transmitting a laser beam 24 having a wavelength of 4 nm, cutting a light having a wavelength of 473 nm, and transmitting a light having a wavelength longer than 473 nm. Instead, only the light in the wavelength range of the photostimulable light 45 emitted from the photostimulable phosphor layer region 12 is transmitted, and 980n
A filter 52e having a property of cutting off light having a wavelength of m
A second dichroic mirror 28 that transmits light having a wavelength of 532 nm or more and reflects light having a wavelength of 473 nm, and transmits a light having a wavelength of 640 nm or less and a wavelength of 980 nm. The scanner shown in FIGS. 7 to 14 has the same configuration except that the third dichroic mirror 56 that reflects the light of FIG.

The scanner according to the present embodiment configured as described above reads the chemiluminescence data recorded in the many stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 as follows. To generate biochemical analysis data.

First, the stimulable phosphor sheet 15 is placed on the glass plate 41 of the stage 40 by the user.

Next, the user operates the keyboard 7
An instruction signal for reading the chemiluminescence data recorded in the large number of stimulable phosphor layer regions 17 formed on the stimulable phosphor sheet 15 is input to 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
8 is moved to pass only the light in the wavelength region of the stimulable light 45 emitted from the photostimulable phosphor layer region 17, and the filter member 51e provided with the filter 52e having the property of cutting the light of the wavelength of 980 nm. Are placed in the optical path of the stimulated emission 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, Of the large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15, the first stimulable phosphor layer region 17 is located at a position where the laser beam 24 can be irradiated.
When it is confirmed that the optical head 35 has reached, a stop signal is output to the main scanning stepping motor 65, and
The drive signal is output to the fourth laser excitation light source 55 to
The laser excitation light source 55 is activated to emit the laser light 24 having a wavelength of 980 nm.

The laser light 24 generated from the fourth laser excitation light source 55 is collimated by the collimator lens 31 and then reflected by the third dichroic mirror 56 to change its direction by 90 degrees. Then, it is incident on the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and focused by the aspherical lens 37 on the first stimulable phosphor layer region 17 of the stimulable phosphor sheet 15 placed on the stage 40 glass plate 41.

In the present embodiment, each of the stimulable phosphor layer regions 17 is a recess 1 formed in a stainless steel support 11 having a property of attenuating light energy.
Since the stimulable phosphor layer is embedded in 3 to be formed, the laser beam 24 is scattered in each stimulable phosphor layer area 17 and adjacent to the stimulable phosphor layer area. It becomes possible to effectively prevent the stimulable phosphor contained in the adjacent stimulable phosphor layer regions 17 from being excited into the inside 17 and being excited.

The laser light 24 causes the stimulable phosphor sheet 15 to emit light.
First stimulable phosphor layer region 1 formed on the support 11 of
When incident on 7, the stimulable phosphor contained in the first stimulable phosphor layer region 17 formed on the stimulable phosphor sheet 15 is excited by the laser light 24 to generate the first stimulable phosphor. The photostimulable light 45 is emitted from the stimulable phosphor layer region 17.

The photostimulable light 45 emitted from the first photostimulable phosphor region 17 is condensed by the aspherical lens 37 provided in the optical head 35, and the same as the optical path of the laser light 24 by the mirror 36. Is reflected to the side and made into parallel light,
It is incident on the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 34 is reflected by the concave mirror 38.
Incident on.

The photostimulable light 45 incident on the perforated mirror 34.
21 is reflected downward by the perforated mirror 34 formed by the concave mirror and enters the filter 52e of the filter unit 48, as shown in FIG.

The filter 52e has a property of transmitting only the light in the wavelength region of the stimulable light emitted from the stimulable phosphor and cutting off the light of the wavelength of 980 nm, and therefore, the excitation light of 980 nm is used. The light of the wavelength is cut off, and only the light in the wavelength range of the photostimulated light passes through the filter 52e and is photoelectrically detected by the photomultiplier 50.

The photomultiplier 50 photoelectrically detects the stimulated emission 45, and the generated analog signal is output to the A / D converter 53 and converted into a digital signal, which is then sent to the data processing device 54. Is output.

After the fourth laser excitation light source 55 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 70 outputs a drive stop signal to the fourth laser excitation light source 55, The drive of the fourth laser excitation light source 55 is stopped, and a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move to the adjacent luminescent elements formed on the support 11 of the stimulable phosphor sheet 15. It is moved by one pitch equal to the distance between the exhaustive phosphor layer regions 17.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is confirmed to have been moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 17, the control unit 70 outputs a drive signal to the fourth laser excitation light source 55. The fourth laser excitation light source 55 is turned on, and the stimulable phosphor sheet 15 is irradiated with the laser light 24.
First stimulable phosphor layer region 1 formed on the support 11 of
The stimulable phosphor contained in the second stimulable phosphor layer region 17 adjacent to 7 is excited.

Similarly, the laser beam 24 is applied to the second photostimulable phosphor layer region 17 formed on the support 11 of the stimulable phosphor sheet 15 for a predetermined time, and the second photostimulable phosphor layer region 17 is irradiated with the second photostimulable phosphor layer region 17.
Photostimulable light 45 emitted from the photostimulable phosphor layer region 17 of
However, when it is photoelectrically detected by the photomultiplier 50, the control unit 70 outputs a drive stop signal to the fourth laser excitation light source 55 to turn off the fourth laser excitation light source 55 and A drive signal is output to the scanning stepping motor 65 to output the optical head 35.
Are moved by one pitch equal to the distance between adjacent photostimulable phosphor layer regions 17.

Thus, the fourth laser excitation light source 55 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and based on the position detection signal of the optical head 35 input from the linear encoder 67, Optical head 35
Is moved by one line in the main scanning direction, and the scanning of the stimulable phosphor layer region 17 of the first line formed on the support 11 of the stimulable phosphor sheet 15 by the laser beam 24 is completed. When it is confirmed, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position and outputs a drive signal to the sub scanning pulse motor 61. , The movable substrate 63 in the sub-scanning direction by one line,
To move.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the stimulable phosphor layer of the first line formed on the support 11 of the stimulable phosphor sheet 15 to be formed. In the same manner as the region 17 is sequentially irradiated with the laser light 24 emitted from the fourth laser excitation light source 55, the brightness of the second line formed on the support 11 of the stimulable phosphor sheet 15 is increased. The stimulable phosphor layer region 1 is sequentially irradiated with the laser light 24 emitted from the fourth laser excitation light source 55, and the stimulable phosphor layer region 1 of the second line is irradiated.
The stimulable phosphor contained in No. 7 is excited, and the stimulable light 45 emitted from the stimulable phosphor layer region 17 is sequentially detected photoelectrically by the photomultiplier 50.

The photomultiplier 50 photoelectrically detects the stimulated emission 45, and the analog data generated is converted into digital data by the A / D converter 53 and sent to the data processing device 54. .

Thus, all the stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 are
The photostimulable phosphor layer region 1 is scanned by the laser beam 24.
The stimulable phosphor contained in 7 is excited, and the emitted stimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is A / D.
When converted into digital data by the converter 53 and sent to the data processing device 54, a drive stop signal is sent from the control unit 70 to the fourth laser excitation light source 55.
And the driving of the fourth laser excitation light source 55 is stopped.

Thus, the radiation data recorded in the large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 is read, and biochemical analysis data is generated.

When the generation of the biochemical analysis data is completed in this way, the biochemical analysis unit 1 is washed.

In the present embodiment, the specific binding substance is adsorbed on the adsorption layer 4 formed on the inner surface 3a of the large number of recesses 3 formed on the substrate 2 of the biochemical analysis unit 1, and the labeling substance The substance of biological origin labeled by
It is hybridized with the specific binding substance contained in the adsorption layer 4, and therefore the biochemical analysis unit 1
In order to wash the biochemical analysis unit 1, the adsorption layer 4 formed on the inner surface 3a of the recess 3 may be washed. Since the adsorption layer 4 has a large surface area, the biochemical analysis unit 1 is efficiently washed. Then, it becomes possible to reuse.

According to this embodiment, the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating radiation energy and light energy. A large number of stimulable phosphor layers formed on the support 11 of the stimulable phosphor sheet 10 by the radioactive labeling substance selectively contained in the adsorption layer 4 formed on the inner surfaces 3a of the large number of recesses 3. When exposing the region 12, the electron beam (β-ray) emitted from the radiolabeled substance is emitted from the substrate 2 of the biochemical analysis unit 1.
Scattering inside is effectively prevented, and each of the stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is formed on the biochemical analysis unit 1. The stimulable phosphor sheet 10 has a diameter equal to that of the corresponding recess 3, and each of the stimulable phosphor layer regions 12 exactly faces the corresponding recess 3 formed in the biochemical analysis unit 1. Since the biochemical analysis unit 1 and the biochemical analysis unit 1 are overlapped and exposed, an electron beam (β-ray) emitted from each adsorption layer 4 is formed on the inner surface 3a of the adjacent recess 3 4 is effectively prevented from reaching the stimulable phosphor layer region 12 facing the stimulable phosphor layer 4. Therefore, even if the recesses 3 are formed in the substrate 2 of the biochemical analysis unit 1 at a high density, A large number of photostimulable fluorescence formed on the support 11 of the sheet 10. The layer region 12, only by radioactive labeling substance contained in the adsorbing layer 4 formed on the inner surface 3a of the corresponding recess 3, it is possible to reliably exposed.

Further, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating radiation energy and light energy, the laser beam 24 is used for biochemical analysis. It is possible to effectively prevent the fluorescent substance such as the fluorescent dye contained in the adsorption layer 4 formed on the inner surfaces 3a of the adjacent recesses from being scattered and scattered in the substrate 2 of the unit 1 to emit fluorescence. Therefore, the laser beam 24 is included in the biochemical analysis data generated by photoelectrically detecting the fluorescence 45.
It is possible to effectively prevent the generation of noise due to the scattering of the, and improve the quantitativeness of the biochemical analysis.

Further, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating radiation energy and light energy, the laser light 24 or the LED light source 100 is used. Irradiation of the emitted excitation light excites a fluorescent substance such as a fluorescent dye, and the emitted fluorescence is effectively prevented from scattering in the substrate 2 of the biochemical analysis unit 1 and is adjacent to each other. Since the fluorescence emitted from the fluorescent substance such as the fluorescent dye contained in the adsorption layer 4 formed on the inner surface 3a of the recess 3 is effectively prevented from being mixed, the substrate 2 of the biochemical analysis unit 1 In addition, even if the recesses 3 are formed at a high density, it is possible to effectively generate noise due to fluorescence scattering in the biochemical analysis data generated by photoelectrically detecting fluorescence. Locks it is possible to improve the quantification of the biochemical analysis.

Furthermore, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having a property of attenuating radiation energy and light energy, it can be brought into contact with a chemiluminescent substrate. By this, chemiluminescence emitted from the labeling substance is effectively prevented from being scattered in the substrate 2 of the biochemical analysis unit 1, and therefore, the adsorption layer 4 formed on the inner surface 3 a of the adjacent recesses 3. Since it is surely prevented that the chemiluminescence emitted from the labeling substance contained in is mixed, the chemiluminescence is generated by photoelectrically detecting the chemiluminescence even if the recesses 3 are formed at a high density in the substrate 2. It is possible to effectively prevent the generation of noise due to the scattering of chemiluminescence in the data for biochemical analysis and improve the quantitativeness of biochemical analysis.

Further, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having the property of attenuating radiation energy and light energy, the substrate of the biochemical analysis unit 1 is Two
The stimulable phosphor layers formed on the support 11 of the stimulable phosphor sheet 15 by chemiluminescence selectively emitted from the adsorption layer 4 formed on the inner surfaces 3a of the recesses 3. When exposing the region 17, the biochemical analysis unit 1
The chemiluminescence selectively emitted from the adsorption layer 4 formed on the inner surfaces 3a of the plurality of recesses 3 of the substrate 2 is effectively prevented from scattering in the substrate 2 of the biochemical analysis unit 1. In addition, each of the stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 has the same diameter as the corresponding recess 3 formed in the biochemical analysis unit 1. The stimulable phosphor sheet 15 and the biochemical analysis unit 1 are overlapped so that each of the stimulable phosphor layer regions 17 exactly faces the corresponding recess 3 formed in the biochemical analysis unit 1. As a result, a large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 are formed by chemiluminescence.
Since the light is exposed, the chemiluminescence emitted from the adsorption layer 4 of the biochemical analysis unit 1 opposes the adsorption layer 4 formed on the inner surface 3a of the adjacent recesses 3 in the stimulable phosphor layer region. 17 is effectively prevented, so that even if the recesses 3 are formed at a high density in the substrate 2 of the biochemical analysis unit 1, a large number of them are formed on the support 11 of the stimulable phosphor sheet 15. The stimulable phosphor layer region 17 can be reliably exposed only by the chemiluminescence emitted from the adsorption layer 4 formed on the inner surface 3 a of the corresponding recess 3.

Further, according to this embodiment, the specific binding substance which is a probe is adsorbed in the adsorption layer 4 formed on the inner surface 3a of each recess 3, and the porous material is contained in the recess 3. Compared to the case where a suction area is formed by embedding
Since it is adsorbed in a small volume region, the reaction rate of hybridization can be improved, and further, the hybridization reaction solution 9 can be brought into contact with each adsorption layer 4 in a sufficiently large area. ,
It is possible to increase the probability that the target-derived substance contained in the hybridization reaction solution 9 will come into contact with the specific binding substance, which is the probe adsorbed in the adsorption layer 4, and thus the hybridization will occur. The efficiency of can be greatly improved.

According to this embodiment, the specific binding substance is adsorbed by the adsorption layer 4 formed on the inner surface 3a of the large number of recesses 3 formed on the substrate 2 and labeled with the labeling substance. The substance derived from the living body is also hybridized with the specific binding substance contained in the adsorption layer 4. Therefore, when the biochemical analysis unit 1 is washed, it is formed on the inner surface 3 a of the recess 3. It is only necessary to wash the existing adsorption layer 4, and since the adsorption layer 4 has a large surface area, the biochemical analysis unit 1 can be efficiently washed and reused.

Further, according to the present embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum, it hardly expands or contracts even when subjected to liquid treatment such as hybridization, and Of the stimulable phosphor layer regions 12, 17 formed on the support 11 of the stimulable phosphor sheets 10, 15 of the corresponding biochemical analysis unit 1 having the adsorption layer 4 formed on the inner surface thereof. The stimulable phosphor layer regions 12, 1 are easily and surely overlapped with the stimulable phosphor sheets 10 and 15 and the biochemical analysis unit 1 so as to accurately face the recesses 3.
7 can be exposed.

FIG. 23 is a schematic partial sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 23, in this embodiment, the biochemical analysis unit 121 has a property of attenuating radiation energy and light energy, is formed of aluminum having flexibility, and has a large number of units. The substrate 122 having the substantially circular recesses 123 formed at a high density is provided.

As shown in FIG. 23, a large number of recesses 12 are formed.
Adsorption layers 124 are formed on the inner surface 123a of No. 3 by nylon 6 capable of forming a membrane filter. In the present embodiment, the surface of the adsorption layer 124 is roughened so as to have a fractal structure, and its adsorption surface area is increased.

Although not shown in FIG. 23, in this embodiment also, about 5000 recesses 123 having a size of about 10,000 square millimeters of about 10,000 are provided.
It is regularly formed on the substrate 122 with a density of square centimeters.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
Similarly, as shown in FIG.
A solution of a large number of specific binding substances such as cDNA is dripped onto the adsorption layer 124 formed on the inner surface 123a of No. 3.

In the biochemical analysis unit 121 according to this embodiment, the surface of the adsorption layer 124 is treated so as to have a fractal structure, and the adsorption surface area thereof is increased. The binding substance can be adsorbed to the adsorption layer 124 formed on the inner surface 123a of each recess 123.

Furthermore, as shown in FIG. 4, chemiluminescence is obtained by contacting a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. The biochemical analysis unit 121 is set in the hybridization reaction container 8 containing the hybridization reaction solution 9 containing the substance of biological origin labeled with the labeling substance that causes
A specific binding substance such as cDNA adsorbed on the adsorption layer 124 formed on 3a is labeled with a radioactive labeling substance and is labeled with a biological substance contained in the hybridization reaction solution 9 or a fluorescent substance such as a fluorescent dye. The biological substance contained in the hybridization reaction solution 9 and the biological substance contained in the hybridization reaction solution 9 and labeled with the labeling substance that causes chemiluminescence are selectively hybridized.

In this way, the biochemical analysis unit 121
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

Fluorescence data recorded in the biochemical analysis unit 121 is recorded in the scanner shown in FIGS. 7 to 14 or FIGS. 15 to 18 in the same manner as in the above embodiment.
The cooled CCD camera 81 of the data generation system shown in FIG.
Is read, and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 12
The radiation data of the radio-labeled substance recorded in No. 1 is transferred to many stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10.

That is, in the same manner as in FIG. 6, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are respectively formed in the biochemical analysis unit 1.
21, the stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 121 so as to face the corresponding recesses 123 formed on the substrate 122, and an adsorption layer formed on the inner surface 123 a of the plurality of recesses 123. A large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are exposed by the radioactive labeling substance selectively contained in 124.

Thus, the radiation data transferred to the stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 is shown in FIGS. 7 to 14 in the same manner as in the above embodiment. The shown scanner will read and generate data for biochemical analysis.

On the other hand, the chemiluminescence data recorded in the biochemical analysis unit 121 is the same as in the above embodiment.
Data is read by the cooled CCD camera 81 of the data generation system shown in FIGS. 15 to 18 to generate data for biochemical analysis, or a large number of bright phosphors of the stimulable phosphor sheet 15 shown in FIG. 19 are generated. It is transferred to the exhaustive phosphor layer region 17.

The chemiluminescence data transferred to the large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 is obtained by the scanner shown in FIGS. 20 to 22 in exactly the same manner as in the above embodiment. It is read and biochemical analysis data is generated.

According to this embodiment, since the substrate 122 of the biochemical analysis unit 121 is formed of aluminum that attenuates radiation energy and light energy, a large number of concave portions of the substrate 122 of the biochemical analysis unit 121 are formed. Due to the radioactive labeling substance selectively contained in the adsorption layer 124 formed on the inner surface 123a of 123, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are formed. During exposure, an electron beam (β-ray) emitted from the radiolabeled substance contained in the adsorption layer 124 formed on the inner surfaces 123a of the large number of recesses 123.
Can be reliably prevented from being scattered within the substrate 122, and the support 11 of the stimulable phosphor sheet 10 can be prevented.
The stimulable phosphor layer regions 12 formed on the substrate are aligned and aligned so as to accurately face the corresponding recesses 123 formed on the substrate 122 of the biochemical analysis unit 121. Since the chemical analysis unit 121 and the stimulable phosphor sheet 10 are superposed, the inner surface 123a of each recess 123 of the biochemical analysis unit 121.
The electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorption layer 124 formed on the substrate is incident only on the corresponding stimulable phosphor layer region 12, and therefore, the biochemical analysis unit 121. The radioactive labeling substance contained in the adsorption layer 124 formed on the inner surface 123a of each recess 123 makes it possible to selectively expose only the corresponding stimulable phosphor layer region 12 to the biochemical region. Analysis unit 1
The regions of the stimulable phosphor layer regions 12 to be exposed by the radioactive labeling substance contained in the adsorption layer 124 formed on the inner surface 123a of each recess 123 of the adjacent recesses 21 are adjacent to each other.
Noise caused by exposure is generated in the biochemical analysis data by the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorption layer 124 formed on the inner surface 123a of 23. This can be prevented and the quantitativeness of biochemical analysis can be significantly improved.

Further, according to this embodiment, since the substrate 122 of the biochemical analysis unit 121 is formed of aluminum that attenuates radiation energy and light energy, a large number of the substrates 122 of the biochemical analysis unit 121 are formed. A large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 by chemiluminescence selectively emitted from the adsorption layer 124 formed on the inner surface 123a of the recess 123 of the. When exposed to light, the adsorption layer 124 formed on the inner surface 123a of the large number of recesses 123
It is possible to reliably prevent the chemiluminescence emitted from the substrate from being scattered in the substrate 122, and to provide a large number of stimulable phosphor layer regions formed on the support 11 of the stimulable phosphor sheet 15. 17 is a biochemical analysis unit 12 respectively
Since the biochemical analysis unit 121 and the stimulable phosphor sheet 15 are superposed on each other so as to accurately face the corresponding recesses 123 formed on the first substrate 122, the biochemical analysis is performed. The chemiluminescence emitted from the adsorption layer 124 formed on the inner surface 123a of each recess 123 of the unit 121 for use corresponds to the corresponding stimulable phosphor layer region 1.
7 only, therefore the biochemical analysis unit 1
By chemiluminescence emitted from the adsorption layer 124 formed on the inner surface 123a of each recess 123 of 21, it becomes possible to selectively expose only the corresponding stimulable phosphor layer region 17, and biochemical analysis Recesses 123 of the unit 121 for use
Photostimulable phosphor layer region 1 to be exposed by chemiluminescence emitted from the adsorption layer 124 formed on the inner surface 123a of the
It is possible to prevent generation of noise in the biochemical analysis data due to exposure of the area 7 by the chemiluminescence emitted from the adsorption layer 124 formed on the inner surface 123a of the adjacent recess 123. It is possible to significantly improve the quantitativeness of biochemical analysis.

Further, according to this embodiment, the specific binding substance which is a probe is adsorbed on the adsorption layer 124 formed on the inner surface 123a of each recess 123 of the biochemical analysis unit 121.
Since the porous material is adsorbed in the inside of the recess 123 and is adsorbed in a region having a small volume as compared with the case where the porous material is embedded to form the adsorption region, the reaction rate of hybridization is improved. Further, since the hybridization solution 9 can be brought into contact with each adsorption layer 124 in a sufficiently large area, the substance derived from the living body, which is the target contained in the hybridization solution 9, is absorbed into the adsorption layer 124. It is possible to increase the probability of encountering the specific binding substance that is the probe adsorbed inside, and therefore, the efficiency of hybridization can be greatly improved.

Further, according to the present embodiment, the specific binding substance is adsorbed on the adsorption layer 124 formed on the inner surface 123a of the large number of recesses 123 formed on the substrate 122 of the biochemical analysis unit 121. The biological substance labeled with the labeling substance is also hybridized with the specific binding substance contained in the adsorption layer 124. Therefore, when the biochemical analysis unit 120 is washed, It is sufficient to wash the adsorption layer 124 formed on the inner surface 123a. Since the adsorption layer 124 has a large surface area, the biochemical analysis unit 121 can be efficiently washed and reused. become.

Further, according to this embodiment, each adsorption layer 12
Since the surface of No. 4 is treated to have a fractal structure and its adsorption surface area is increased, it is possible to adsorb a sufficient amount of the specific binding substance.

FIG. 24 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.
5 is a partial sectional view thereof.

As shown in FIGS. 24 and 25, the biochemical analysis unit 131 according to this embodiment has a property of attenuating radiation energy and light energy and is formed of flexible aluminum. Substrate 1 in which a large number of substantially circular through holes 133 are formed at high density
32 are provided.

As shown in FIG. 25, a large number of through holes 1
The inner surface 133a of 33 is made of nitrocellulose capable of forming a membrane filter.
34 is formed.

Although not shown exactly in FIG. 24, in the present embodiment, the through hole 133 having a size of about 0.01 square millimeter of about 10,000 is about 500.
Substrate 1 regularly with a density of 0 pieces / square centimeter
32 is formed.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
Similarly, as shown in FIG.
In the adsorption layer 134 formed on the inner surface 133a of 33, c
After a solution containing a specific binding substance such as DNA is dropped, as shown in FIG. 4, a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, etc. A biochemical analysis unit 131 is inserted into a hybridization reaction container 8 containing a hybridization reaction solution 9 containing a substance of biological origin labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate. The specific binding substance such as cDNA adsorbed on the adsorption layer 134 formed on the inner surface 133a of the large number of through holes 133 is labeled with a radiolabeling substance and is derived from the living body contained in the hybridization reaction solution 9. Hybridization reaction solution 9 labeled with a fluorescent substance such as a substance or a fluorescent dye Labeled by causing substances and chemiluminescent from contained biological indicator substance, a substance derived from a living organism contained in the hybridization reaction solution 9, selectively, are hybridized.

In this way, the biochemical analysis unit 131
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

The fluorescence data recorded in the biochemical analysis unit 131 is the same as in the above embodiment, and the scanner shown in FIGS. 7 to 14 or FIGS.
The cooled CCD camera 81 of the data generation system shown in FIG.
Is read, and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 13
The radiation data of the radio-labeled substance recorded in No. 1 is transferred to many stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10.

That is, in the same manner as in FIG. 6, a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are respectively formed in the biochemical analysis unit 1.
The stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 131 so as to face the corresponding through holes 133 formed in the substrate 132 of No. 31, and a large number of through holes 13 are formed.
Of the stimulable phosphor sheet 1 by the radioactive labeling substance contained in the adsorption layer 134 formed on the inner surface 133a of
A large number of photostimulable phosphor layer regions 12 formed on the support 11 of 0 are exposed.

Thus, the radiation data transferred to the large number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are shown in FIGS. The data is read by the scanner shown in 14 and biochemical analysis data is generated.

On the other hand, the chemiluminescence data recorded in the biochemical analysis unit 131 is the same as in the above embodiment.
A large number of stimulable phosphors of the stimulable phosphor sheet 15 shown in FIG. 19 are read by the cooled CCD camera 81 of the data generation system shown in FIGS. 15 to 18 or in the same manner as in the above embodiment. It is transferred to the phosphor layer region 17.

The chemiluminescence data transferred to the large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 are obtained by the scanner shown in FIGS. 20 to 22 in exactly the same manner as in the above embodiment. It is read and biochemical analysis data is generated.

According to this embodiment, since the substrate 132 of the biochemical analysis unit 131 is made of aluminum that attenuates radiation energy and light energy, it is formed on the substrate 132 of the biochemical analysis unit 131. When a large number of stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are exposed by the radioactive labeling substance selectively contained in the corresponding large number of through holes 133, a large number of Inner surface 13 of through hole 133 of
It is possible to effectively prevent the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorption layer 134 formed on the substrate 3a from being scattered in the substrate 132, and it is also possible to accumulate fluorescence. A number of stimulable phosphor layer regions 12 formed on the support 11 of the body sheet 10 are respectively accurately opposed to the corresponding through holes 133 formed on the substrate 132 of the biochemical analysis unit 131. Aligned,
The stimulable phosphor sheet 10 is a biochemical analysis unit 131.
Since it is superposed on the biochemical analysis unit 13,
The electron beam (β-ray) emitted from the radiolabel substance contained in the adsorption layer 134 formed on the inner surface 133a of each through hole 133 of No. 1 is incident only on the corresponding stimulable phosphor layer region 12. Therefore, the biochemical analysis unit 131
The radioactive labeling substance contained in the adsorption layer 134 formed on the inner surface 133a of each of the through holes 133 makes it possible to selectively expose only the stimulable phosphor layer region 12 corresponding thereto. The regions of the stimulable phosphor layer region 12 to be exposed by the radioactive labeling substance contained in the adsorption layer 134 formed on the inner surface 133a of each through hole 133 of the chemical analysis unit 131 are adjacent to the through holes 133. That noise due to exposure is generated in the biochemical analysis data by the electron beam (β-ray) emitted from the radiolabel substance contained in the adsorption layer 134 formed on the inner surface 133a of the Can be prevented and the quantitativeness of biochemical analysis can be significantly improved.

Further, according to this embodiment, since the substrate 132 of the biochemical analysis unit 131 is made of aluminum that attenuates radiation energy and light energy, it is formed on the substrate 132 of the biochemical analysis unit 131. From the corresponding large number of through holes 133,
When a large number of stimulable phosphor layer regions 17 formed on the support 11 of the stimulable phosphor sheet 15 are exposed to light by selectively emitted chemiluminescence, they are formed on the inner surface 133a of a large number of through holes 133. The chemiluminescence emitted from the adsorbed layer 134 can be effectively prevented from being scattered in the substrate 132, and a large number of bright phosphors formed on the support 11 of the stimulable phosphor sheet 15 can be formed. The exhaustive phosphor layer region 17 is
Each is aligned so that the stimulable phosphor sheet 15 is superposed on the biochemical analysis unit 131 so as to accurately face the corresponding through hole 133 formed on the substrate 132 of the biochemical analysis unit 131. Because
The chemiluminescence emitted from the adsorption layer 134 formed on the inner surface 133a of each through hole 133 of the biochemical analysis unit 131 is incident only on the corresponding stimulable phosphor layer region 17, and therefore the biochemical analysis is performed. By chemiluminescence emitted from the adsorption layer 134 formed on the inner surface 133a of each through hole 133 of the unit 131, only the corresponding stimulable phosphor layer region 17 can be selectively exposed,
The region of the photostimulable phosphor layer region 17 to be exposed by the chemiluminescence emitted from the adsorption layer 134 formed on the inner surface 133a of each through hole 133 of the biochemical analysis unit 131 is adjacent to the through hole 133. The chemiluminescence emitted from the adsorption layer 134 formed on the inner surface 133a can prevent noise due to exposure from being generated in the biochemical analysis data, and quantify the biochemical analysis. It is possible to greatly improve the property.

Further, according to the present embodiment, the specific binding substance as the probe is adsorbed on the inner surface 133a of each through hole 133 of the biochemical analysis unit 131 by the adsorption layer 13 formed thereon.
4 is adsorbed in the through hole 133, and the porous material is embedded in the through hole 133 to adsorb in a region having a smaller volume than in the case where the adsorption region is formed. Can be improved and further
Since the hybridization solution 9 can be brought into contact with each adsorption layer 134 in a sufficiently large area, the substance derived from the living body which is the target contained in the hybridization solution 9 is adsorbed in the adsorption layer 134. It is possible to increase the probability of encountering a specific binding substance that is a probe, and therefore, the efficiency of hybridization can be significantly improved.

Further, according to this embodiment, the specific binding substance is adsorbed by the adsorption layer 134 formed on the inner surface 133a of the large number of through holes 133 formed on the substrate 132 and labeled with the labeling substance. The substance derived from the living body is also hybridized with the specific binding substance contained in the adsorption layer 134. Therefore, when the biochemical analysis unit 131 is washed, it is formed on the inner surface 133 a of the through hole 133. It is only necessary to wash the adsorbed layer 134 that has been adsorbed, and since the adsorbed layer 134 has a large surface area, the biochemical analysis unit 131 can be efficiently washed and reused.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above-mentioned embodiment, a plurality of cDNAs having different known base sequences are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to the cDNA. It is not limited to cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, RNAs, etc. Any specific binding substance whose base sequence, base length, composition, etc. are known can be used as the specific binding substance of the present invention.

Furthermore, in the above embodiment, the substrates 2, 122 of the biochemical analysis units 1, 121, 131,
Although 132 is formed of aluminum, the substrates 2 and 12 of the biochemical analysis units 1, 121 and 131 are formed.
It is not always necessary to form 2, 132 with aluminum, and the biochemical analysis units 1, 121,
The substrates 2, 122, 132 of 131 may be made of a material having a property of attenuating radiation energy and / or light energy. The material for forming the substrates 2, 122, 132 of the biochemical analysis units 1, 121, 131 is not particularly limited,
It may be formed of either an inorganic compound material or an organic compound material, and particularly preferably, a metal material, a ceramic material or a plastic material forms the substrates 2, 122 and 132 of the biochemical analysis units 1, 121 and 131. It is used to Biochemical analysis unit 1, 12
Examples of the inorganic compound material used to form the substrates 1, 122 and 132 of 1, 131 include gold,
Metals such as silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, Examples thereof include silicon materials such as silicon nitride; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, a polymer compound is preferably used as the organic compound material, and examples of the polymer compound include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers;
Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon 4,10 Nylon such as; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, Cellulose acetate, Nitrocellulose, Starch, Alginic acid Polysaccharides such as calcium and hydroxypropyl methylcellulose; chitin; chitosan; sumacum; gelatin, collagen, kerula Polyamides such emissions and the like can be mentioned copolymers of these polymeric compounds.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

In the above embodiment, the substrates 2, 122, 132 of the biochemical analysis unit 1 are made of aluminum having a property of attenuating radiation energy and light energy, but the stimulable phosphor sheet is used. When only the radiation data recorded in the large number of photostimulable phosphor layer regions 12 generated on the support 11 of 10 are read to generate the biochemical analysis data, the biochemical analysis unit 1, Substrates 2 and 12 of 121 and 131
2, 132 may be formed of a material having a property of transmitting light energy but attenuating radiation energy, while the substrate 2, 122 of the biochemical analysis unit 1, 121, 131 is formed. When only the chemiluminescence data or fluorescence data recorded in the adsorption layers 4, 124, and 134 formed on 132 are read to generate the biochemical analysis data, the biochemical analysis units 1, 121, 131 are used. The substrates 2, 122 and 132 of the biochemical analysis units 1, 121 and 131 can be formed of a material having a property of transmitting radiation energy but attenuating light energy.
It is not always necessary that 2,132 be formed of a material having a property of attenuating radiation energy and light energy.

Further, in the embodiment shown in FIGS. 1 and 2 and the embodiment shown in FIG. 23, about 1
The approximately circular recesses 3,123 having a size of about 0.01 square millimeters of 0000 are regularly formed on the substrate 2,122 at a density of about 5000 pieces / square centimeter and are shown in FIGS. 24 and 25. In another embodiment, substantially circular through holes 133 having a size of about 0.01 square millimeters of about 10,000 are regularly formed on the substrate 132 at a density of about 5000 holes / square centimeter, 3, 123 and through hole 133
However, it is not always necessary that the recesses 3 and 123 and the through-hole 133 are formed in a substantially circular shape, and the recesses 3 and 123 and the through hole 133 can be formed in any shape, for example, a rectangular shape.

In the embodiment shown in FIGS. 1 and 2 and the embodiment shown in FIG. 23, about 10
The approximately circular recesses 3,123 having a size of about 0.01 square millimeters of about 3, 000 are regularly formed on the substrate 2, 122 at a density of about 5000 per square centimeter and are shown in FIGS. In another embodiment, substantially circular through holes 133 having a size of about 0.01 square millimeters of about 10,000 are regularly formed on the substrate 132 at a density of about 5000 holes / square centimeter, 3, 123 or through hole 133
The number and size of the substrate 2 can be arbitrarily selected according to the purpose, and preferably the number of the recesses 3, 123 or the number of the through holes 133 is 10 or more and the substrate 2, 122, 132, the recesses 3, 123 or the through holes 133 have a size of less than 5 mm 2, and the biochemical analysis units 1, 121,
It is formed on the substrates 2, 122 and 132 of 131.

Further, in the embodiment shown in FIGS. 1 and 2 and the embodiment shown in FIG. 23, about 1
The approximately circular recesses 3,123 having a size of about 0.01 square millimeters of 0000 are regularly formed on the substrate 2,122 at a density of about 5000 pieces / square centimeter and are shown in FIGS. 24 and 25. In another embodiment, substantially circular through holes 133 having a size of about 0.01 square millimeters of about 10,000 are regularly formed on the substrate 132 at a density of about 5000 holes / square centimeter, 3, 123 or through hole 133
Regularly, biochemical analysis units 1, 121, 13
It is not always necessary to form one substrate 2, 122, 132.

Also, in the above-mentioned embodiment, chemiluminescence is generated by contacting with a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. A hybridization reaction solution 9 containing a substance of biological origin labeled with a labeling substance to be prepared is prepared, and a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemistry A biological substance labeled with a labeling substance that causes chemiluminescence when brought into contact with a luminescent substrate is hybridized with a specific binding substance adsorbed on the adsorption layers 4, 124 and 134. The substance of biological origin contained in the solution 9 is a radioactive labeling substance, a fluorescent color. Fluorescent substance and not necessarily required that is labeled by a labeling substance which generates chemiluminescent emission when it contacts a chemiluminescent substrate, such as,
It may be labeled with at least one labeling substance selected from the group consisting of a labeling substance that causes chemiluminescence by contacting with a radioactive labeling substance, a fluorescent substance and a chemiluminescent substrate.

Further, in the above-mentioned embodiment, a substance derived from a living body which is labeled with a radioactive labeling substance, a fluorescent substance such as a fluorescent dye, and a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate is specifically bound. Although it is hybridized to the substance, it is not always necessary to hybridize the substance of biological origin to the specific binding substance, and the substance of biological origin is replaced by hybridization, and an antigen-antibody reaction, a receptor. It is also possible to specifically bind to a specific binding substance by a reaction with a ligand or the like.

Further, in the above embodiment, a large number of recesses 3 formed in the substrates 2, 122, 132 of the biochemical analysis unit 1 are formed on one surface of the support 11 of the stimulable phosphor sheets 10, 15. , 123 or through holes 133, and a large number of substantially circular stimulable phosphor layer regions 12, 17 are regularly formed with the same size as the recesses 3, 123 or through holes 133. But
It is not always necessary to form a large number of stimulable phosphor layer regions 12, 17 on one surface of the support 11 of the stimulable phosphor sheet 10, and the support 11 for the stimulable phosphor sheets 10, 15 is not necessarily required. The stimulable phosphor layer may be uniformly formed on one surface of the above.

In the above embodiment, the biochemical analysis units 1, 121, 131 have the substrates 2, 12 on one surface of the support 11 of the stimulable phosphor sheets 10, 15.
A large number of substantially circular stimulable phosphor layer regions 12, 17 are formed in the concave portions 3, 12 in the same pattern as that of the large number of concave portions 3, 123 or the through holes 133 formed in the concave portions 3, 12.
3 or a size equal to that of the through holes 133, and are regularly formed, but a large number of stimulable phosphor layer regions 12, 17 are formed.
Is a large number of recesses 3, 123 formed in the substrates 2, 122, 132 of the biochemical analysis units 1, 121, 131.
Alternatively, in the same pattern as the pattern of the through holes 133,
If it is formed, it is not necessary that it is regularly formed.

Further, in the above-mentioned embodiment, a large number of recesses 3 formed in the substrates 2, 122, 132 of the biochemical analysis unit 1 are formed on one surface of the support 11 of the stimulable phosphor sheets 10, 15. , 123 or through holes 133, and a large number of substantially circular stimulable phosphor layer regions 12, 17 are regularly formed with the same size as the recesses 3, 123 or through holes 133. But
It is not always necessary that the large number of stimulable phosphor layer regions 12, 17 are formed in a substantially circular shape, and the stimulable phosphor layer regions 12, 17 are formed in an arbitrary shape, for example, a rectangular shape. You can also

Further, in the above-mentioned embodiment, a large number of recesses 3 formed in the substrates 2, 122, 132 of the biochemical analysis unit 1 are formed on one surface of the support 11 of the stimulable phosphor sheets 10, 15. , 123 or through holes 133, and a large number of substantially circular stimulable phosphor layer regions 12, 17 are formed in the recesses 3, 123 or through holes 1.
The stimulable phosphor layer region 1 of the stimulable phosphor sheets 10 and 15 is formed regularly with a size equal to 33.
It is not always necessary to form 2, 17 in the same size as the recesses 3, 123 or the through holes 133, and the size of the stimulable phosphor layer regions 12, 17 may be set as necessary.
You can decide. Preferably, the stimulable phosphor layer region 12 has the same size as the recesses 3, 123 or the through holes 133, or the recesses 3, 123 or the through holes 1.
The stimulable phosphor sheets 10 and 15 are formed to have a size larger than 33.

Furthermore, in the above embodiment, a large number of stimulable phosphor layer regions 1 of the stimulable phosphor sheets 10 and 15 are used.
2 and 17 are a large number of recesses 13 formed in the support 11.
, A stimulable phosphor is embedded and formed,
It is not always necessary to form a large number of photostimulable phosphor layer regions 12 and 17 by embedding the photostimulable phosphor in the large number of recesses 13 formed in the support 11, and a large number of photocatalysts may be formed in the support 11. A large number of through holes may be formed, and a large number of through holes may be filled with or filled with a stimulable phosphor to form a large number of stimulable phosphor layer regions 12 and 17, or on the surface of the support 11. Alternatively, a large number of stimulable phosphor layer regions 12 and 17 may be formed.

Furthermore, in the above embodiment, the support 11 of the stimulable phosphor sheets 10 and 15 is made of stainless steel, but it is not always necessary to form the support 11 of stainless steel. It is also possible to form the support 11 of the stimulable phosphor sheets 10 and 15 by using the above material. Storage phosphor sheet 10, 15
The support 11 is preferably made of a material having a property of attenuating radiation energy and light energy, but is not particularly limited.
The support 11 of the stimulable phosphor sheets 10 and 15 can be formed of either an inorganic compound material or an organic compound material, and a metal material, a ceramic material or a plastic material is particularly preferably used. Examples of the inorganic compound material that can be preferably used to form the support 11 of the stimulable phosphor sheets 10 and 15 and that can attenuate radiation energy and light energy include gold, silver, copper, zinc, and aluminum. , Metals such as titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; Aluminum oxide,
Examples thereof include metal oxides such as magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. In addition, the stimulable phosphor sheet 1
Polymer compounds are preferably used as the organic compound material that can be preferably used to form the support 11 of 0 or 15 and that can attenuate the radiation energy and the light energy. Polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethylmethacrylate and butyl acrylate / methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotriethylene Fluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon such as nylon 6, nylon 6,6 and nylon 4,10; polyimide; Risuruhon; phenolic resins such as novolak; silicon resins such as polydiphenylsiloxane; polyphenylene sulfide epoxy resin;
Polyurethane; polystyrene; butadiene-styrene copolymers; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan;
Urushi; polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds can be mentioned. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Further, in the embodiment shown in FIGS. 24 and 25, the through hole 133 formed in the substrate 132 is used.
The adsorption layer 134 is formed on the inner surface 133a of nitrocellulose using a nitrocellulose capable of forming a membrane filter. However, as in the embodiment shown in FIG.
The surface of 34 may be roughened so as to have a fractal structure.

Further, in the embodiment shown in FIG. 23, the surface of the adsorption layer 124 formed on the inner surface 123a of each recess 123 is roughened so as to have a fractal structure. The surface of the adsorption layer 124 does not necessarily need to have a fractal structure, and may be roughened such as a multi-protrusion structure or a micropore structure.

Further, in the embodiment shown in FIGS. 1 and 2 and the embodiments shown in FIGS. 24 and 25, the adsorption layers 4, 1 of the biochemical analysis units 1, 131 are formed.
34 is formed of nitrocellulose, and in the embodiment shown in FIG. 23, the biochemical analysis unit 1
The adsorption layer 124 of No. 21 is made of nylon 6, but it is not always necessary that the adsorption layers 4, 124 and 134 are made of nitrocellulose or nylon 6, and the adsorption layers 124, 134 and 134 may be made of other adsorption materials. The adsorption layers 4, 124 of the chemical analysis units 1, 121, 131,
It is also possible to form 134. A porous material or a fiber material is preferably used as the adsorptive material for forming the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131, and the porous material and the fiber material are used in combination. It is also possible to form the adsorption layers 4, 124 and 134 of the biochemical analysis units 1, 121 and 131. Adsorption layers 4, 12 of biochemical analysis units 1, 121, 131
The porous material for forming 4,134 is an organic material,
Either an inorganic material or an organic / inorganic composite may be used. The organic porous material used for forming the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131 is not particularly limited, but a carbon porous material such as activated carbon or a membrane. A porous material capable of forming a filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6, nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof. . The inorganic porous material used for forming the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131 is not particularly limited, but preferably, for example, platinum, gold, Examples thereof include metals such as iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof. The fiber material used for forming the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131 is not particularly limited, but preferably, for example, nylon 6 or nylon 6 is used. , 6, nylons such as nylon 4 and 10, cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate and the like.

Furthermore, in the above-mentioned embodiment, by using the scanner shown in FIG. 7 to FIG. 14, the radioactive labeling substance recorded in a large number of stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 is recorded. A large number of adsorption layers 4, 124 of the radiation data and biochemical analysis units 1, 121, 131,
The fluorescence data of fluorescent substances such as fluorescent dyes recorded in 134 are read to generate biochemical analysis data. However, the radiation data of radiolabeled substances and the fluorescence data of fluorescent substances are not read by a single scanner. It is not always necessary to read the radiation data of the radiolabeled substance and the fluorescence data of the fluorescent substance with separate scanners to generate the biochemical analysis data.

Further, in the above-mentioned embodiment, the radiation data of the radiolabeled substance recorded in the large number of stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 and the biochemical analysis unit 1, 121. 7 to FIG. 14, when the fluorescence data of the fluorescent substance such as the fluorescent dye recorded in the large number of adsorption layers 4, 124, and 134 of the biosensors 131 and 131 is read to generate the data for biochemical analysis. When the chemiluminescence data recorded in a large number of stimulable phosphor layer regions 17 of the stimulable phosphor sheet 15 is read by using a scanner to generate biochemical analysis data, refer to FIGS. Although the illustrated scanner is used, radiation data recorded on the stimulable phosphor sheet 10, chemiluminescence data recorded on the stimulable phosphor sheet 15, or a biochemical analysis unit , 121, 131 as the scanner for reading the fluorescence data recorded in the stimulable phosphor layer regions 12, 17 of the stimulable phosphor sheets 10, 15 by the laser beam 24 or other excitation light. It suffices as long as it can scan a large number of adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 132 to excite the stimulable phosphor or the fluorescent substance, and FIGS. The radiation data recorded on the stimulable phosphor sheet 10 or the fluorescence data recorded on the biochemical analysis units 1, 121, 131 is read using the scanner shown in FIG. It is not always necessary to read the chemiluminescence data recorded on the stimulable phosphor sheet 15 using a scanner.

Further, in the above-mentioned embodiment, the scanner shown in FIGS. 7 to 14 is provided with the first laser excitation light source 21, the second laser excitation light source 22 and the third laser excitation light source 23. The scanner shown in FIGS. 20 to 22 includes a first laser excitation light source 21, a second laser excitation light source 22 and a fourth laser excitation light source 55, but the scanner includes three laser excitation light sources. It is not always necessary.

Further, in the above embodiment, the biochemical analysis units 1 and 12 are provided by the data generation system shown in FIGS.
1, 131 multiple recesses 3, 123 or through holes 13
The chemiluminescence data of the labeling substance that causes chemiluminescence by contacting with the chemiluminescence substrate recorded on the adsorption layers 4, 124, and 134 formed on the inner surfaces 3a, 123a, and 133a of No. 3 are read to perform biochemical analysis. However, it is not always necessary that the data generation system that reads the chemiluminescence data and generates the biochemical analysis data can also generate the fluorescence data. , Biochemical analysis unit 1,
Chemiluminescence is generated by bringing into contact with the chemiluminescent substrate recorded in the adsorption layers 4, 124, 134 formed on the inner surfaces 3a, 123a, 133a of the multiple recesses 3, 123 of the 121, 131 or the through holes 133. When used to read only the chemiluminescence data of the labeling substance, the LED light source 100, the filter 101, the filter 1
02 and the diffusion plate 103 can be omitted.

Further, in the above embodiment, the scanner shown in FIGS. 7 to 14 and FIGS.
In the scanner shown in FIG. 2, by the scanning mechanism, the optical head 35 is moved in the main scanning direction indicated by the arrow X and the sub scanning direction indicated by the arrow Y in FIG. Phosphor sheet 1
The stimulable phosphor or fluorescent dye is scanned by scanning all the stimulable phosphor layer regions 12, 17 of 0, 15 or all the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131. 13 is configured to excite a fluorescent substance, but the optical head 35 is kept stationary, and the stage 40 is moved in the main scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG. To the stimulable phosphor layer regions 12, 17 of the stimulable phosphor sheets 10, 15 by moving the laser beam 24.
Alternatively, all the adsorption layers 4, 124, 134 of the biochemical analysis units 1, 121, 131 may be scanned to excite a fluorescent substance such as a stimulable phosphor or a fluorescent dye. In FIG. 13, the head 35 is moved in the main scanning direction indicated by the arrow X or in the sub scanning direction indicated by the arrow Y, and the stage 40 is moved.
Can be moved in the sub-scanning direction indicated by arrow Y or in the main-scanning direction indicated by arrow X.

Further, in the scanner shown in FIGS. 7 to 14 and the scanner shown in FIGS. 20 to 22, a photomultiplier 50 is used as a photodetector, and fluorescence or stimulated emission is photoelectrically converted. However, the photodetector used in the present invention is not limited to the photomultiplier 50 as long as it can detect fluorescence or stimulated emission photoelectrically, and may be a line CCD or a two-dimensional CCD. Other photodetectors can also be used.

Further, in the above embodiment, the spotting device 5 having the injector 6 and the CCD camera 7 is provided.
Using the CCD camera 7, while observing the tip of the injector 6 and the recesses 3, 123 or the through holes 133 to which the solution containing the specific binding substance such as cDNA should be dropped, the tip of the injector 6 and the cDNA When the center of the recesses 3, 123 or the through-holes 133 to which the solution containing the specific binding substance such as is to be dropped is matched, the injector 6 releases the solution of the specific binding substance such as cDNA and then drops the solution. However, the relative positional relationship between the tip portion of the injector 6 and the recessed portion 3123 or the through hole 133 formed in the biochemical analysis unit 1, 121 or 131 is detected in advance, and the injector 6
And the biochemical analysis units 1, 121 and 131 are relatively moved two-dimensionally at a constant pitch, and the cD
It is also possible to configure so that a solution containing a specific binding substance such as NA is dropped.

[0361]

EFFECTS OF THE INVENTION According to the present invention, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. are formed. By forming a high density on the substrate and specifically binding the substance of biological origin labeled with a radioactive labeling substance to the specific binding substance contained in the multiple spot-shaped regions, the multiple spot-shaped regions are formed. The biochemical analysis unit obtained by selectively labeling is brought into close contact with the stimulable phosphor layer, and the stimulable phosphor layer is exposed to a radioactive labeling substance, and the stimulable phosphor layer is excited by an excitation light. The photostimulable light emitted from the stimulable phosphor layer is photoelectrically detected to generate data for biochemical analysis, and when the substance derived from the living body is analyzed, it is emitted from the radiolabeled substance. The noise caused by the scattering of the electron beam (β-ray) that is generated is the data for biochemical analysis. It is possible to provide a biochemical analysis unit which can be prevented from being generated during.

Further, according to the present invention, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like are formed. , Specific binding substance that is formed on the substrate at high density and that causes chemiluminescence by contacting the specific binding substance contained in multiple spot-like regions with the chemiluminescent substrate Chemiluminescence is released by contacting a chemiluminescent substrate with a biochemical analysis unit obtained by selectively labeling multiple spot-like regions by chemically binding them. The analysis unit is brought into close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed by chemiluminescence, and the stimulable phosphor layer is irradiated with excitation light to produce the stimulable phosphor layer. The photostimulable light emitted from the A biochemical analysis unit that can prevent noise due to chemiluminescence scattering from being generated in biochemical analysis data even when biological analysis data is generated and a biological substance is analyzed. It becomes possible to provide.

Furthermore, according to the present invention, it is possible to specifically bind to a substance of biological origin, and the base sequence and base length,
A plurality of spot-shaped regions containing a specific binding substance having a known composition etc. is formed on a substrate at high density, and the specific binding substance contained in the plurality of spot-shaped regions is added to the radiolabeled substance, or In place of the radiolabeled substance, a substance derived from a living body labeled with a labeling substance and / or a fluorescent substance that produces chemiluminescence by contacting with a chemiluminescent substrate is specifically bound and selectively labeled. The chemiluminescence or fluorescence emitted from the obtained biochemical analysis unit is photoelectrically detected to generate biochemical analysis data, and when a substance derived from a living body is analyzed, by contacting with a chemiluminescent substrate, Noise generated by chemiluminescence emitted from a labeling substance that causes chemiluminescence or scattering of fluorescence emitted from a fluorescent substance is generated in the data for biochemical analysis. It is possible to provide a biochemical analysis unit which can be prevented.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a schematic partial cross-sectional view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 3 is a schematic front view of a spotting device.

FIG. 4 is a schematic cross-sectional view of a hybridization reaction container.

FIG. 5 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 6 is a diagram showing the formation of a stimulable phosphor sheet on a support by a radiolabel substance contained in an adsorption layer formed on the inner surfaces of a large number of recesses formed on a substrate of a biochemical analysis unit. FIG. 6 is a schematic cross-sectional view showing a method of exposing a large number of stimulable phosphor layer regions thus formed.

FIG. 7 is a schematic perspective view showing an example of a scanner.

FIG. 8 is a schematic perspective view showing details of a scanner near the photomultiplier.

9 is a schematic cross-sectional view taken along the line AA of FIG.

10 is a schematic cross-sectional view taken along the line BB of FIG.

11 is a schematic cross-sectional view taken along the line CC of FIG.

12 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 13 is a schematic plan view of a scanning mechanism of an optical head.

14 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner shown in FIG.

FIG. 15 is a schematic front view of a data generation system.

FIG. 16 is a cooling CCD of the data generation system.
It is a schematic longitudinal cross-sectional view of a camera.

FIG. 17 is a schematic vertical sectional view of a dark box of the data generation system.

FIG. 18 is a block diagram of the periphery of the personal computer of the data generation system.

FIG. 19 is a schematic perspective view of a stimulable phosphor sheet to which chemiluminescence data recorded in a number of absorptive regions formed in a biochemical analysis unit is to be transferred.

FIG. 20 is a view showing a biochemical analysis by reading chemiluminescence data recorded in a large number of stimulable phosphor layer regions formed on a support of the stimulable phosphor sheet shown in FIG. FIG. 3 is a schematic perspective view of a scanner that generates usage data.

FIG. 21 is a schematic perspective view showing details of the scanner near the photomultiplier of the scanner shown in FIG. 20.

FIG. 22 is a schematic cross-sectional view taken along the line EE of FIG.

FIG. 23 is a schematic partial cross-sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 24 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 25 is a schematic partial cross-sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical Analysis Unit 2 Substrate 3 Recess 3a Inner Surface of Recess 4 Adsorption Layer 5 Spotting Device 6 Injector 7 CCD Camera 8 Hybridization Container 9 Hybridization Solution 10 Storage Phosphor Sheet 11 Support 12 Photostimulable Phosphor Layer Area 13 Recess 15 Accumulative phosphor sheet 17 Photostimulable phosphor layer area 21 First laser excitation light source 22 Second laser excitation light source 23 Third laser excitation light source 24 Laser light 25 Collimator lens 26 Mirror 27 First Dichroic mirror 28 Second dichroic mirror 29 Mirror 30 Collimator lens 31 Collimator lens 32 Mirror 33 Perforated mirror hole 34 Perforated mirror 35 Optical head 36 Mirror 37 Aspherical lens 38 Concave mirror 40 Stage 41 Glass plate 45 Fluorescent or stimulated Light 48 Filter unit 50 Photomultipliers 51a, 51b, 51c, 51d, 51e Filter members 52a, 52b, 52c, 52d, 52e Filter 53 A / D converter 54 Data processing device 55 Fourth laser excitation light source 56 Third dichroic mirror 60 Substrate 61 Sub-scanning pulse motor 62 Pair of rails 63 Movable substrate 64 Rod 65 Main scanning stepping motor 66 Endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 Keyboard 72 Filter unit motor 81 Cooling CCD camera 82 Dark box 83 Personal computer 84 CRT 85 Keyboard 86 CCD 87 Heat transfer plate 88 Peltier element 89 Shutter 90 A / D converter 91 Image data buffer 92 Camera control circuit 9 5 glass plate 96 radiating fin 97 camera lens 100 LED light source 101 filter 102 filter 103 diffusion plate 110 CPU 111 data transfer means 112 data storage means 113 data processing device 114 data display means 115 light source control means 121 biochemical analysis unit 122 substrate 123 Recessed portion 123a Recessed inner surface 124 Adsorption layer 131 Biochemical analysis unit 132 Substrate 133 Through hole 133a Inner surface of through hole 134 Adsorption layer

Claims (25)

[Claims] 1. A substrate, which is formed of a material that attenuates radiation energy and / or light energy and has a plurality of holes formed therein, wherein an adsorption layer is formed on an inner surface of the plurality of holes. Biochemical analysis unit. 2. A substrate formed of a material that attenuates radiation energy and / or light energy and having a plurality of holes formed therein, wherein an adsorption layer is formed on an inner surface of the plurality of holes formed in the substrate. A labeling substance that causes chemiluminescence by dropping a specific binding substance having a known structure or property into the plurality of pores formed in the substrate and contacting with a radioactive labeling substance, a fluorescent substance and a chemiluminescence substrate A substance derived from a living body, which is labeled with at least one labeling substance selected from the group consisting of the following, is specifically bound to the specific binding substance, and the adsorption layer is selectively labeled. A unit for biochemical analysis. 3. The specific substance is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor / ligand. The biochemical analysis unit described in. 4. The biochemical analysis unit according to claim 1, wherein the plurality of holes are formed by recesses. 5. The biochemical analysis unit according to any one of claims 1 to 3, wherein the plurality of holes are formed by through holes. 6. The adsorption layer according to claim 1, wherein the adsorption layer is made of a porous material.
The unit for biochemical analysis according to the item. 7. The biochemical analysis unit according to claim 6, wherein the adsorption layer is formed of a carbon porous material or a porous material capable of forming a membrane filter. 8. The biochemical analysis unit according to claim 1, wherein the adsorption layer is made of a fiber material. 9. The biochemical analysis according to any one of claims 1 to 5, wherein the adsorption layer is formed by subjecting the substrate to a surface treatment with a surface modifier. unit. 10. The biochemical analysis unit according to claim 9, wherein the adsorption layer is formed by subjecting the substrate to a surface treatment with a silane coupling agent. 11. The biochemical analysis unit according to claim 1, wherein the surface of the adsorption layer is roughened. 12. The biochemical analysis unit according to claim 11, wherein the surface of the adsorption layer is roughened so as to have a fractal structure. 13. The biochemical analysis unit according to claim 1, wherein 10 or more holes are formed. 14. The biochemical analysis unit according to claim 13, wherein the holes are formed in 1000 or more. 15. The biochemical analysis unit according to claim 14, wherein the holes are formed in 10,000 or more. 16. The biochemical analysis unit according to claim 1, wherein the size of the hole is less than 5 mm 2. 17. The biochemical analysis unit according to claim 16, wherein the size of the hole is less than 1 mm 2. 18. The biochemical analysis unit according to claim 17, wherein the size of the hole is less than 0.1 mm 2. 19. The hole is formed with a density of 10 holes / cm 2 or more.
19. The unit for biochemical analysis according to any one of 1 to 18. 20. The biochemical analysis unit according to claim 19, wherein the holes are formed with a density of 1000 holes / square centimeter or more. 21. The biochemical analysis unit according to claim 20, wherein the holes are formed with a density of 10,000 or more per cm 2. 22. A material for attenuating the radiation and / or light energy, the radiation and / or light being transmitted through the material by a distance equal to the distance between adjacent adsorbent layers. 22. The biochemical analysis unit according to any one of claims 1 to 21, which has a property of attenuating light energy to 1/5 or less. 23. The radiation and / or light energy is attenuated when the radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent layers. 23. The biochemical analysis unit according to claim 22, which has a property of attenuating light energy to 1/10 or less. 24. Radiation and / or light when the radiation and / or light energy attenuating material penetrates the material by a distance equal to the distance between adjacent adsorption layers. 24. The biochemical analysis unit according to claim 23, which has a property of attenuating light energy to 1/100 or less. 25. The biochemical analysis according to claim 22, wherein the substrate is formed of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. unit.