JP2003227833A - Method for association reaction between receptor and ligand and reactor used for the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a reactor capable of efficiently bringing a ligand or a receptor fixed to a unit for biochemical analysis into association reaction with a receptor or a ligand and creating data for biochemical analysis with superior reproducibility and quantitativeness. <P>SOLUTION: The method for the association reaction is characterized in that a plurality of adsorbent regions containing the receptor or the ligand are formed separately from one another in the unit for biochemical analysis, and the reactor is provided with a reaction container provided with a unit holding part for holding the unit for biochemical analysis. The reaction container is provided with a first electrode group 14 comprised of a plurality of linear electrodes on one side and a second electrode group 15 comprised of a plurality of linear electrodes on the other side, and the plurality of linear electrodes constituting the first electrode group and the plurality of linear electrodes constituting the second electrode group are arranged in such a way as to intersect with each other. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a receptor-ligand association reaction method and a reactor used therefor, and more specifically, to a ligand or receptor immobilized on a biochemical analysis unit, efficiently,
The present invention relates to a receptor-ligand association reaction method and a reactor used for the method, which allows a receptor or a ligand to undergo an association reaction and can generate data for biochemical analysis with excellent reproducibility and excellent quantification.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a specific binding substance such as protein or nucleic acid is immobilized on a biochemical analysis unit such as a membrane filter and is labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A substance derived from a living body is specifically bound to a specific binding substance,
Visible light generated by contact between the chemiluminescent substrate and the labeling substance by bringing the chemiluminescent substrate into contact with the binding substance of the specific binding substance selectively labeled with the labeling substance and the substance of biological origin The chemiluminescence in the wavelength range is photoelectrically detected, a digital signal is generated, image processing is performed, and C
A chemiluminescence analysis system is also known in which a chemiluminescence image is displayed on a display means such as RT or a recording material such as a photographic film to obtain information on a substance of biological origin such as gene information.

Furthermore, in recent years, cells at different positions on the surface of a carrier such as a slide glass plate or a membrane filter,
Viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA
A specific binding substance, such as A, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Forming a large number of independent spots, then cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c
A substance derived from a living body, such as DNA, DNA, or mRNA, which is collected from the living body by extraction or isolation, or which is further subjected to a chemical treatment, a chemical modification, or the like, such as a fluorescent substance or a dye. A substance labeled with a labeling substance is bound to a specific binding substance by hybridization or the like, to a microarray that is specifically bound,
A microarray analysis system has been developed which irradiates excitation light and photoelectrically detects light such as fluorescence emitted from a labeling substance such as a fluorescent substance or a dye to analyze a substance derived from a living body. According to this microarray analysis system,
A large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and the substance of biological origin labeled with the labeling substance is hybridized, thus In time, there is an advantage that it is possible to analyze a substance of biological origin.

In addition, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, RNAs, etc. derived from living organisms are located at different positions on the surface of a carrier such as a membrane filter. The specific binding substance that can specifically bind to the substance of which the base sequence, base length, composition, etc. are known is dropped using a spotter device to form a large number of independent spots. , Then, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc., collected from the living body by extraction or isolation, or
Furthermore, a substance derived from a living body, which has been subjected to a chemical treatment, a chemical modification, or the like, and which is labeled with a radioactive labeling substance, is specifically bound to a specific binding substance by hybridization or the like. Macro array,
The stimulable phosphor layer containing the stimulable phosphor is brought into close contact with the stimulable phosphor sheet, and the stimulable phosphor layer is exposed.
After that, the photostimulable phosphor layer is irradiated with excitation light, the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected, and biochemical analysis data is generated. A macroarray analysis system using a radiolabeled substance for analyzing is also developed.

[0008]

In a microarray analysis system or a macroarray analysis system, a solution containing a specific binding substance is dropped at different positions on the surface of a biochemical analysis unit such as a membrane filter, and a large number of A specific substance contained in the spot-like region is a biological substance labeled with a radioactive substance, a fluorescent substance, or a labeling substance that causes chemiluminescence when contacted with a chemiluminescent substrate. By hybridizing with the binding substance, the specific binding substance is selectively labeled, and the stimulable phosphor layer of the stimulable phosphor sheet is changed by the radioactive labeling substance selectively contained in a large number of spot-shaped regions. Exposed, the exposed photostimulable phosphor layer, scanned by excitation light, to excite the photostimulable phosphor contained in the photostimulable phosphor layer, Photostimulated photostimulable light emitted from the photostimulable phosphor is generated photoelectrically to generate data for biochemical analysis, or a large number of spot-shaped regions are scanned with excitation light to form a large number of spot-shaped regions. Exciting the fluorescent substance selectively contained, photoelectrically detecting the fluorescence emitted from the fluorescent substance, to generate biochemical analysis data, or
It is required to generate data for biochemical analysis by contacting a chemiluminescent substrate with a labeling substance selectively contained in many spot-like regions and photoelectrically detecting chemiluminescence emitted from the labeling substance. Has been done.

In the case of hybridizing a specific binding substance with a substance of biological origin, conventionally, an experimenter manually performed biochemistry such as a membrane filter in which a large number of spot-shaped regions containing the specific binding substance were formed. The analysis unit is placed in a hybridization bag, and a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contacting with a radioactive labeling substance, a fluorescent substance, or a chemiluminescent substrate is placed in the hybridization bag. Add a hybridization solution containing the product, and apply vibration to the hybridization bag to move the substance of biological origin by convection or diffusion to hybridize the specific binding substance and the substance of biological origin. Remove from hybridization bag and fill with wash solution Placed in the vessel for cleaning was generally.

However, the experimenter manually puts the unit for biochemical analysis in the hybridization bag, adds the hybridization solution, and vibrates the hybridization bag so that the specific binding substance and the biological origin can be obtained. When hybridizing the substance of
It is difficult to uniformly contact the hybridization solution with a large number of spot-shaped regions containing the specific binding substance, and therefore the specific binding substance and the substance of biological origin cannot be efficiently hybridized. There was a problem.

Further, the experimenter manually puts the unit for biochemical analysis in the hybridization bag, adds the hybridization solution, and vibrates the hybridization bag so that the specific binding substance and the biogenic substance are derived. Hybridize the substance of the above, remove the unit for biochemical analysis from the hybridization bag,
When the washing solution is placed in a container filled with a washing solution and washed, it is unavoidable that the results of hybridization vary depending on the experimenter and the reproducibility is deteriorated.
Even the same experimenter has a problem that the reproducibility may decrease.

[0012] An antigen or an antibody is fixed to a biochemical analysis unit such as a membrane filter, and a ligand and a receptor are associated with each other as in the case of binding the antibody or the antigen to the fixed antigen or the antibody by an antigen-antibody reaction. In the case of reacting, there is a similar problem. Furthermore, a target DNA fixed to a biochemical analysis unit such as a membrane filter is hybridized with a probe DNA labeled with a hapten such as digoxigenin, and further chemiluminescence Antibodies to haptens such as digoxigenin that are labeled with an enzyme that causes chemiluminescence when contacted with a substrate, and haptens such as digoxigenin that is labeled with an enzyme that has the property of producing a fluorescent substance when contacted with a fluorescent substrate. antibody , The antigen-antibody reaction, by binding to a hapten that is labeled probes DNA, even when the target DNA is labeled, there is a similar problem.

Therefore, according to the present invention, the ligand or receptor immobilized on the biochemical analysis unit can be efficiently associated with the receptor or the ligand, and the reproducibility and the quantification are excellent. Receptor that makes it possible to generate data for chemical analysis
An object of the present invention is to provide a ligand association reaction method and a reactor used therefor.

[0014]

The object of the present invention is to:
Multiple absorptive regions containing receptors or ligands
A biochemical analysis unit formed separately from each other, a reaction container containing a reaction solution containing a ligand or a receptor labeled with a labeling substance, and a plurality of linear electrodes constituting a first electrode group. , A plurality of linear electrodes constituting the second electrode group, at a position corresponding to the plurality of absorptive regions of the biochemical analysis unit, so as to intersect, the aforesaid arranged in the reaction vessel Set between the first electrode group and the second electrode group, and apply a positive or negative voltage to at least one line electrode of the plurality of line electrodes forming the first electrode group. In addition, a negative or positive voltage is applied to at least one line-shaped electrode of the plurality of line-shaped electrodes that form the second electrode group, so that the reaction solution contained in the reaction vessel is melted. Ligand or receptor contained in the, to cross the plurality of absorptive regions formed in the biochemical analysis unit, forcibly moved,
While applying a negative or positive voltage to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes that form the first electrode group, the second electrode group A positive or negative voltage is applied to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes included in the reaction solution contained in the reaction container. The ligands or receptors present in the biochemical analysis unit are repeatedly moved by forcibly moving them so as to cross the plurality of absorptive regions formed in the biochemical analysis unit. Selectively react with the receptor or ligand contained in the reaction region by the ligand or receptor contained in the reaction solution. It is achieved by receptor-ligand association reaction method which is characterized in that to.

In the present invention, the receptor-ligand association reaction includes a hybridization reaction and an antigen-antibody reaction.

According to the present invention, a biochemical analysis unit in which a plurality of absorptive regions containing a receptor or a ligand are formed apart from each other is contained in a reaction solution containing the ligand or the receptor labeled with a labeling substance. In the reaction vessel, a plurality of line-shaped electrodes that form the first electrode group and a plurality of line-shaped electrodes that form the second electrode group are provided in the plurality of absorptive regions of the biochemical analysis unit. At a corresponding position, so as to intersect, a first electrode group arranged in the reaction vessel, and at least a plurality of line-shaped electrodes that are set between the second electrode group and constitute the first electrode group. A positive or negative voltage is applied to one line electrode, and a negative or positive voltage is applied to at least one line electrode of the plurality of line electrodes forming the second electrode group. In addition, the ligand or receptor contained in the reaction solution housed in the reaction vessel is forcibly moved so as to cross a plurality of absorptive regions formed in the biochemical analysis unit. A plurality of line-shaped electrodes forming a second electrode group while applying a negative or positive voltage to at least one line-shaped electrode different from at least one line-shaped electrode forming the plurality of line-shaped electrodes At least one linear electrode different from at least one linear electrode of the linear electrode,
By applying a positive or negative voltage, the ligand or receptor contained in the reaction solution contained in the reaction vessel is forced to cross multiple absorptive regions formed in the biochemical analysis unit. And the receptor or ligand contained in the plurality of absorptive regions of the biochemical analysis unit is selectively associated with the ligand or receptor contained in the reaction solution. Since only the ligand or receptor contained in the reaction solution is forcibly moved across the plurality of absorptive regions formed in the biochemical analysis unit, the ligand or receptor contained in the reaction solution is Receptors or ligands simply moved by convection or diffusion and contained in the adsorptive region of the biochemical analysis unit. The rate of ligand or receptor migration in the adsorptive region of the biochemical analysis unit can be significantly increased as compared with the case where they are allowed to associate with each other, and thus the reaction rate of the receptor-ligand association reaction is significantly increased. In addition, the probability that the receptor or ligand contained in the deep part of the adsorptive region of the biochemical analysis unit will associate with the ligand or receptor contained in the reaction solution is significantly increased. Therefore, the receptor or ligand contained in the plurality of adsorptive regions of the biochemical analysis unit and the ligand or receptor contained in the reaction solution can be associated with each other as desired. hand,
It becomes possible to react.

Further, by using a pump or the like, the reaction solution is circulated in the reaction vessel through the solution circulation pipe,
When flowing across multiple absorptive regions formed in the biochemical analysis unit, it is necessary to fill the reaction vessel and the solution circulation line with the reaction solution, and the concentration of the reaction components is high. Although it is difficult to use the reaction solution, according to the present invention, simply, at least one line electrode of the plurality of line electrodes forming the first electrode group and the plurality of line electrodes forming the second electrode group are used. By simply applying an opposite voltage to at least one of the linear electrodes of the above, the ligands or receptors contained in the reaction solution are forced to cross the multiple adsorptive regions of the biochemical analysis unit. Therefore, the reaction vessel with a small volume is filled with the reaction solution having a high concentration of the reaction components and is contained in the plurality of absorptive regions of the biochemical analysis unit. To that receptor or ligand,
The ligand or receptor contained in the reaction solution can be allowed to undergo an association reaction, and thus the reaction rate of the receptor-ligand association reaction can be significantly increased.

In a preferred embodiment of the present invention, two of the plurality of line-shaped electrodes constituting the first electrode group are formed.
A positive or negative voltage is applied to the above line electrodes, and a negative or positive voltage is applied to two or more line electrodes of the plurality of line electrodes forming the second electrode group. , Forcibly moving the ligand or receptor contained in the reaction solution contained in the reaction container so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, While applying a negative or positive voltage to two or more line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes that form one electrode group,
A positive or negative voltage is applied to two or more line-shaped electrodes that are different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes that form the second electrode group, and are housed in the reaction container. The step of forcibly moving the ligand or receptor contained in the reaction solution so as to cross the plurality of absorptive regions formed in the biochemical analysis unit is repeated for the biochemical analysis. It is configured to selectively associate the ligands or receptors contained in the reaction solution with the receptors or ligands contained in the plurality of adsorptive regions of the unit.

According to a preferred embodiment of the present invention, a positive or negative voltage is applied to two or more line electrodes of the plurality of line electrodes forming the first electrode group, and the second electrode group is applied. A negative or positive voltage is applied to two or more line-shaped electrodes of the plurality of line-shaped electrodes that make up the ligand or receptor contained in the reaction solution housed in the reaction vessel for biochemical analysis. Two or more line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes that are forcibly moved so as to cross the plurality of absorptive regions formed in the unit Is applied to the two or more line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes forming the second electrode group, Applying an INUS voltage to forcibly move the ligand or receptor contained in the reaction solution contained in the reaction vessel so as to cross the multiple absorptive regions formed in the biochemical analysis unit. By repeating the step of allowing the ligands or receptors contained in the reaction solution to selectively react with the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit,
Only the ligands or receptors that are configured to associate and that are included in the reaction solution are forced to move across the multiple adsorbent regions formed in the biochemical analysis unit and are therefore included in the reaction solution. The ligands or receptors that are present, simply by convection or diffusion,
Significantly increases the migration rate of the ligand or receptor within the adsorptive region of the biochemical analysis unit as compared with the case where it is allowed to associate with the receptor or ligand contained in the adsorptive region of the biochemical analysis unit. Therefore, it becomes possible to significantly increase the reaction rate of the receptor-ligand association reaction,
Furthermore, the probability that the receptor or ligand contained in the deep part of the adsorptive region of the biochemical analysis unit and the ligand or receptor contained in the reaction solution associate can be greatly increased, and therefore, As desired, the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit and the ligands or receptors contained in the reaction solution can be allowed to associate and react with each other. .

Further, using a pump or the like, the reaction solution is circulated in the reaction vessel through the solution circulation line,
When flowing across a plurality of absorptive regions formed in the biochemical analysis unit, it is necessary to fill the reaction vessel and the solution circulation pipeline with the reaction solution, and the concentration of the reaction components is high. Although it is difficult to use the reaction solution, according to the present invention, simply, two or more linear electrodes of the plurality of linear electrodes forming the first electrode group and a plurality of linear electrodes forming the second electrode group are used. The ligand or receptor contained in the reaction solution is forced to cross multiple adsorptive regions of the biochemical analysis unit by simply applying opposite voltages to two or more line electrodes of Therefore, it is possible to fill the inside of a reaction container with a small volume with a reaction solution having a high concentration of reaction components, and then to the receptors included in a plurality of absorptive regions of the biochemical analysis unit. The ligand, ligand or receptor contained in the reaction solution can be the association reaction, therefore, it is possible to greatly increase the reaction rate of the receptor-ligand association reaction.

[0021] In a further preferred aspect of the present invention, two or more line-shaped electrodes, which are not adjacent to each other, of the plurality of line-shaped electrodes forming the first electrode group,
A positive or negative voltage is applied, and a negative or positive voltage is applied to two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the second electrode group, The ligand or receptor contained in the reaction solution contained in the reaction container is forcibly moved so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, and the first A negative or positive voltage is applied to two or more line-shaped electrodes that are different from the two or more line-shaped electrodes that are not adjacent to each other in the plurality of line-shaped electrodes that form the electrode group, and the second electrode A plurality of line-shaped electrodes that are different from the above-mentioned two or more line-shaped electrodes of the plurality of line-shaped electrodes that form a group are not adjacent to each other, plus or minus A voltage of eggplant is applied to force the ligand or receptor contained in the reaction solution housed in the reaction vessel so as to cross the plurality of absorptive regions formed in the biochemical analysis unit. The step of selectively moving the biochemical analysis unit to selectively associate the ligands or receptors contained in the reaction solution with the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit. Is configured.

According to a further preferred aspect of the present invention, a positive or negative voltage is applied to two or more line-shaped electrodes which are not adjacent to each other of the plurality of line-shaped electrodes which form the first electrode group. , A negative or positive voltage is applied to two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the second electrode group, and are contained in the reaction solution housed in the reaction container. Ligands or receptors, so as to cross the multiple absorptive regions formed in the biochemical analysis unit,
The negative or positive voltage is applied to two or more non-adjacent line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes forming the first electrode group by forcibly moving. At the same time, two or more line-shaped electrodes which are different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes which form the second electrode group are not adjacent to each other,
By applying a positive or negative voltage, the ligand or receptor contained in the reaction solution contained in the reaction vessel is forced to cross multiple absorptive regions formed in the biochemical analysis unit. And the receptor or ligand contained in the plurality of absorptive regions of the biochemical analysis unit is selectively associated with the ligand or receptor contained in the reaction solution. Since only the ligand or receptor contained in the reaction solution is forcibly moved across the plurality of absorptive regions formed in the biochemical analysis unit, the ligand or receptor contained in the reaction solution is Receptors or ligands simply moved by convection or diffusion and contained in the adsorptive region of the biochemical analysis unit. The rate of ligand or receptor migration in the adsorptive region of the biochemical analysis unit can be significantly increased as compared with the case where they are allowed to associate with each other, and thus the reaction rate of the receptor-ligand association reaction is significantly increased. In addition, the probability that the receptor or ligand contained in the deep part of the adsorptive region of the biochemical analysis unit will associate with the ligand or receptor contained in the reaction solution is significantly increased. Therefore, the receptor or ligand contained in the plurality of adsorptive regions of the biochemical analysis unit and the ligand or receptor contained in the reaction solution can be associated with each other as desired. hand,
It becomes possible to react.

Further, according to a further preferred aspect of the present invention, a positive or negative voltage is applied to two or more line-shaped electrodes which are not adjacent to each other of the plurality of line-shaped electrodes which form the first electrode group. In addition, a negative or positive voltage is applied to two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the second electrode group, so that the reaction solution contained in the reaction container is Two or more of the plurality of line-shaped electrodes constituting the first electrode group by forcibly moving the contained ligand or receptor so as to cross the plurality of absorptive regions formed in the biochemical analysis unit. The negative or positive voltage is applied to two or more non-adjacent line-shaped electrodes different from the line-shaped electrode of FIG. A positive or negative voltage is applied to two or more line-shaped electrodes that are different from the two or more line-shaped electrodes that are not adjacent to each other and are contained in the reaction solution housed in the reaction container. Since the ligand or receptor is configured to be forcedly moved so as to cross a plurality of absorptive regions formed in the biochemical analysis unit, the ligand or receptor is treated as if the reaction solution was stirred. Are homogeneously mixed in the reaction solution, so that the ligands or receptors contained in the reaction solution and the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit have almost equal probability. Since it becomes possible to associate with each other, the receptors or the receptors contained in the plurality of absorptive regions of the biochemical analysis unit can be The ligand, the ligand or receptor contained in the reaction solution,
It becomes possible to associate and react.

Further, using a pump or the like, the reaction solution is circulated in the reaction vessel through the solution circulation line,
When flowing across a plurality of absorptive regions formed in the biochemical analysis unit, it is necessary to fill the reaction vessel and the solution circulation pipeline with the reaction solution, and the concentration of the reaction components is high. Although it is difficult to use the reaction solution, according to the present invention, simply, two or more non-adjacent linear electrodes of the plurality of linear electrodes forming the first electrode group and the second electrode The reaction solution is made to cross a plurality of absorptive regions of the biochemical analysis unit only by applying an opposite voltage to two or more line electrodes that are not adjacent to each other of the plurality of line electrodes that form the group. Since it is possible to forcibly move the ligand or receptor contained in the
A reaction solution having a high concentration of reaction components can be filled to allow the ligands or receptors contained in the reaction solution to associate with the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit. Therefore, it becomes possible to greatly increase the reaction rate of the receptor-ligand association reaction.

In a preferred embodiment of the present invention, the reaction solution comprises at least one label selected from the group consisting of a radiolabeling substance, a fluorescent substance and a labeling substance which produces chemiluminescence by contacting with a chemiluminescent substrate. It contains a ligand or receptor labeled with a substance.

In a preferred embodiment of the present invention, a unit for biochemical analysis in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are separated from each other is labeled with a labeling substance. The first electrode group in a reaction container containing a reaction solution containing a substance derived from a living body, and the plurality of lines that are set between the second electrode group and constitute the first electrode group. At least one of the electrodes
Applying a positive or negative voltage to one line electrode, applying a negative or positive voltage to at least one line electrode of the plurality of line electrodes forming the second electrode group, A substance of biological origin labeled with a labeling substance contained in the reaction solution is forcibly moved so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, At least one different from the at least one line-shaped electrode of the plurality of line-shaped electrodes forming the electrode group
While applying a negative or positive voltage to one line electrode, at least one line electrode different from the at least one line electrode of the plurality of line electrodes forming the second electrode group, By applying a positive or negative voltage, the substance of biological origin labeled by the labeling substance contained in the reaction solution is made to cross the plurality of absorptive regions formed in the biochemical analysis unit. The step of forcibly moving is repeated to selectively bind the substance of biological origin labeled with a labeling substance to the specific binding substance contained in the plurality of absorptive regions of the biochemical analysis unit. , Configured to hybridize.

In a preferred embodiment of the present invention, a reaction in which an antibody or antigen labeled with a labeling substance comprises a biochemical analysis unit in which a plurality of adsorptive regions containing the antigen or antibody are formed separately from each other. At least one line-shaped electrode of the plurality of line-shaped electrodes that is set between the first electrode group and the second electrode group in the reaction container containing the solution to form the first electrode group. A positive or negative voltage is applied to the electrodes, and a negative or positive voltage is applied to at least one line electrode of the plurality of line electrodes forming the second electrode group to form the reaction solution. The antibody or antigen labeled with the labeling substance contained in the biochemical analysis unit is forcibly transferred so as to cross the plurality of absorptive regions formed in the biochemical analysis unit. And applying a negative or positive voltage to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes forming the first electrode group, A labeling substance contained in the reaction solution by applying a positive or negative voltage to at least one line electrode different from the at least one line electrode of the plurality of line electrodes forming the electrode group. The step of forcibly moving the antibody or antigen labeled with the biochemical analysis unit across the plurality of absorptive regions formed in the biochemical analysis unit is repeated to obtain the plurality of biochemical analysis units. Antigen or antibody contained in the adsorptive region, the antibody or antigen labeled with the labeling substance, by an antigen-antibody reaction, It is configured to be coupled to 択的.

In a preferred embodiment of the present invention, a hapten is used to label a biochemical analysis unit in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are separated from each other. The first electrode group in the reaction container containing a reaction solution containing a substance of biological origin, and the plurality of linear lines that are set between the second electrode group to form the first electrode group At least one of the electrodes
Applying a positive or negative voltage to one line electrode, and applying a negative or positive voltage to at least one line electrode of the plurality of line electrodes forming the second electrode group, A substance derived from a living body labeled by a hapten contained in the reaction solution, so as to traverse the plurality of absorptive regions formed in the biochemical analysis unit, repeating the step of forcibly moving, The specific binding substance contained in the plurality of adsorptive regions of the biochemical analysis unit is selectively hybridized with the substance derived from the living body labeled with a hapten, and further labeled with a labeling enzyme. An antibody solution containing the antibody against the hapten,
A positive or negative voltage is applied to at least one line-shaped electrode of the plurality of line-shaped electrodes that are housed in the reaction vessel and that form the first electrode group, and the second electrode group is formed. By applying a negative or positive voltage to at least one of the plurality of linear electrodes, the antibody against the hapten labeled with the labeling enzyme contained in the reaction solution is subjected to the biochemical analysis. At least different from the at least one line-shaped electrode of the plurality of line-shaped electrodes that are forcibly moved so as to traverse the plurality of absorptive regions formed in the unit for use and form the first electrode group. A negative or positive voltage is applied to one line-shaped electrode, and the plurality of line-shaped electrodes forming the second electrode group are At least one linear electrode different from at least one linear electrode is applied with a positive or negative voltage to generate an antibody against the hapten labeled with the labeling enzyme contained in the reaction solution, Repeating the step of forcibly moving so as to cross the plurality of absorptive regions formed in the chemical analysis unit, the hapten contained in the plurality of absorptive regions of the biochemical analysis unit, labeled It is configured to bind the enzyme-labeled antibody by an antigen-antibody reaction.

In the present invention, examples of the hapten / antibody combination include digoxigenin / anti-digoxigenin antibody, theophylline / anti-theophylline antibody, fluorescein / anti-fluorescein antibody and the like.
It is also possible to use a combination of biotin / avidin, antigen / antibody, etc., instead of the hapten / antibody.

[0030] In a further preferred aspect of the present invention, a biochemical analysis unit in which a plurality of absorptive regions containing a specific binding substance having a known structure or property are separated from each other is labeled with a hapten. The first electrode group in a reaction container containing a reaction solution containing a substance derived from a living body, and the plurality of lines that are set between the second electrode group and constitute the first electrode group. A positive or negative voltage is applied to at least one line-shaped electrode of the linear electrodes, and a negative or positive voltage is applied to at least one line-shaped electrode of the plurality of line-shaped electrodes forming the second electrode group. And a substance derived from a living body labeled with a hapten contained in the reaction solution is applied to the plurality of adsorptive regions formed in the biochemical analysis unit. So that the specific binding substance contained in the plurality of absorptive regions of the biochemical analysis unit, the substance derived from the living body labeled with a hapten is repeated. Selectively, hybridizing, and further contacting with a chemiluminescent substrate, an antibody solution containing an antibody against the hapten labeled with an enzyme that produces chemiluminescence is housed in the reaction vessel, and A positive or negative voltage is applied to at least one line-shaped electrode of the plurality of line-shaped electrodes that form one electrode group, and at least one of the plurality of line-shaped electrodes that forms the second electrode group is applied. By applying a negative or positive voltage to the two linear electrodes, the above-mentioned labeled by the enzyme contained in the reaction solution is added. Antibodies to heptene, to cross the plurality of absorptive regions formed in the biochemical analysis unit, forcibly moved,
While applying a negative or positive voltage to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes that form the first electrode group, the second electrode group A positive or negative voltage is applied to at least one linear electrode different from the at least one linear electrode of the plurality of linear electrodes, which is labeled with an enzyme contained in the reaction solution. Repeating the step of forcibly moving the antibody against the hapten so as to traverse the plurality of absorptive regions formed in the biochemical analysis unit, the plurality of absorptivity of the biochemical analysis unit. It is configured to bind the enzyme-labeled antibody to the hapten contained in the region by the antigen-antibody reaction. That.

In another preferred embodiment of the present invention, a hapten is used to label a unit for biochemical analysis in which a plurality of absorptive regions containing specific binding substances having known structures or characteristics are formed apart from each other. The first electrode group in the reaction container containing a reaction solution containing a substance derived from the living body, is set between the second electrode group, the plurality of the first electrode group to configure A positive or negative voltage is applied to at least one line electrode of the line electrodes, and a negative or positive voltage is applied to at least one line electrode of the plurality of line electrodes forming the second electrode group. By applying a voltage, a substance derived from a living body labeled with a hapten contained in the reaction solution, the plurality of absorptive regions formed in the biochemical analysis unit To traverse, repeating the step of forcibly moving, to the specific binding substance contained in the plurality of absorptive regions of the biochemical analysis unit, the substance derived from the living body labeled with a hapten, Alternatively, by hybridizing and further contacting with a fluorescent substrate, an antibody solution containing an antibody against the hapten labeled with an enzyme that produces a fluorescent substance is housed in the reaction container, and the first A positive or negative voltage is applied to at least one line electrode of the plurality of line electrodes forming the electrode group, and at least one line of the plurality of line electrodes forming the second electrode group. By applying a negative or positive voltage to the electrode, the hapten labeled with the enzyme contained in the reaction solution is applied. At least one of the plurality of line-shaped electrodes constituting the first electrode group is forcibly moved so as to traverse the plurality of absorptive regions formed in the biochemical analysis unit. At least one different from one line electrode
While applying a negative or positive voltage to one line electrode, at least one line electrode different from the at least one line electrode of the plurality of line electrodes forming the second electrode group, By applying a positive or negative voltage, the antibody to the hapten labeled by the enzyme contained in the reaction solution, so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, By repeating the step of forcibly moving, the hapten contained in the plurality of adsorptive regions of the biochemical analysis unit, the antibody labeled with the enzyme, by an antigen-antibody reaction, configured to bind There is.

[0032] In a preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of holes separated from each other, and the plurality of absorptive regions are formed on the substrate. The adsorptive material filled in the plurality of pores is made to contain a receptor or a ligand.

In a further preferred aspect of the present invention, the biochemical analysis unit includes a substrate in which a plurality of through holes are formed apart from each other, and the plurality of absorptive regions are formed in the substrate. The receptor material or the ligand is contained in the absorptive material filled in the plurality of through-holes thus formed.

In a further preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit have an absorptive film containing an absorptive material in a plurality of through holes formed in the substrate. Is press-fitted, and the adsorptive membrane is made to contain a receptor or a ligand.

[0035] In a further preferred aspect of the present invention, the biochemical analysis unit includes a substrate having a plurality of recesses spaced apart from each other, and the plurality of absorptive regions are formed on the substrate. Further, the adsorptive material filled in the plurality of recesses is made to contain a receptor or a ligand.

In another preferred aspect of the present invention, the biochemical analysis unit is configured such that an absorptive substrate and a plurality of through-holes are formed separately from each other, and at least one surface of the absorptive substrate is formed. A plurality of absorptive regions are formed by closely contacting the substrates, and the absorptive substrates in the plurality of through holes formed in the substrate are made to contain a receptor or a ligand.

[0037] In a preferred aspect of the present invention, the biochemical analysis unit is formed with 10 or more absorptive regions.

[0038] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 50 or more absorptive regions.

[0039] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 100 or more absorptive regions.

[0040] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 500 or more absorptive regions.

[0041] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 1000 or more absorptive regions.

[0042] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 5,000 or more absorptive regions.

[0043] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 10,000 or more absorptive regions.

[0044] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 50,000 or more absorptive regions.

[0045] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 100,000 or more absorptive regions.

[0046] In a preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 1 mm 2.

[0048] In a further preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 0.5 mm 2.

[0049] In a further preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 0.1 mm 2.

[0050] In a further preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 0.05 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions of the biochemical analysis unit has a size of less than 0.01 mm 2.

In a preferred embodiment of the present invention, the plurality of absorptive regions are provided in the biochemical analysis unit,
It is formed with a density of 10 pieces / square centimeter or more.

[0053] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 50 or more per cm 2.

[0054] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 100 or more per cm 2.

[0055] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 500 or more per cm 2.

[0056] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 1,000 or more per cm 2.

[0057] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 5,000 or more per cm 2.

[0058] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 10,000 or more per cm 2.

[0059] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 50,000 or more per cm 2.

[0060] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 100,000 per square centimeter or more.

[0061] In a preferred aspect of the present invention, the plurality of absorptive regions are provided in the biochemical analysis unit,
It is formed in a regular pattern.

[0062] In a preferred aspect of the present invention, the plurality of through holes are formed in the substrate of the biochemical analysis unit in a substantially circular shape.

In the present invention, a plurality of absorptive regions of the biochemical analysis unit are formed by containing a receptor or a ligand in an absorptive material filled in a plurality of holes formed in the substrate. Alternatively, if the plurality of absorptive regions of the biochemical analysis unit are formed by incorporating a receptor or a ligand into the absorptive substrate in the plurality of through holes formed in the substrate, it is preferable. ,
The substrate of the biochemical analysis unit has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating radiation, an absorptive region is formed at a high density in the biochemical analysis unit. , The specific binding substances contained in the multiple adsorptive regions of the biochemical analysis unit are hybridized with the substance of biological origin labeled with a radioactive labeling substance, selectively labeled, and then used for biochemical analysis. The unit and the stimulable phosphor sheet on which the stimulable phosphor layer is formed are superposed and formed on the support of the stimulable phosphor sheet by the radioactive labeling substance selectively contained in a plurality of absorptive regions. When the stimulable phosphor layer was exposed to light and the radiation data was recorded on the stimulable phosphor layer of the stimulable phosphor sheet, it was also released from the radioactive labeling substance contained in each absorptive region. Electron beam (β-ray) It can be effectively prevented that scattering in the substrate of the unit for chemical analysis, therefore,
The electron beam (β-ray) emitted from the radiolabeled substance contained in each adsorptive region is selectively made incident on the region of the corresponding stimulable phosphor layer to generate the corresponding stimulable phosphor. Since it becomes possible to expose only the region of the layer, the photostimulable phosphor layer exposed by the radiolabel substance is scanned with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically converted. It is possible to generate data for biochemical analysis with high resolution and high quantification by performing the automatic detection.

[0065] In a preferred aspect of the present invention, when the substrate of the biochemical analysis unit is exposed to radiation by a distance equal to the distance between the adjacent absorptive regions, the radiation is Energy of 1/5
It has the following property of damping.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, The energy of radiation
It has the property of being attenuated to 1/10 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit has radiation transmitted through the substrate by a distance equal to the distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/50 or less.

[0068] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, The energy of radiation
It has the property of being attenuated to 1/100 or less.

[0069] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to the distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/500 or less.

[0070] In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits radiation through the substrate by a distance equal to a distance between the adjacent absorptive regions, The energy of radiation
It has the property of being attenuated to 1/1000 or less.

In the present invention, when the plurality of absorptive regions of the biochemical analysis unit are formed by containing a receptor or a ligand in the absorptive material filled in the plurality of holes formed in the substrate Alternatively, if the plurality of absorptive regions of the biochemical analysis unit are formed by incorporating a receptor or a ligand into the absorptive substrate in the plurality of through holes formed in the substrate, it is preferable. ,
The substrate of the biochemical analysis unit has a property of attenuating light.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating light, the absorptive regions are densely formed on the substrate of the biochemical analysis unit. Selectively binds a substance of biological origin that is formed and labeled with a labeling substance that causes chemiluminescence by contacting a specific binding substance contained in a plurality of adsorptive regions with a fluorescent substance or a chemiluminescent substrate. , Even when combined, it is effective that the fluorescence and chemiluminescence emitted from a large number of adsorptive regions are scattered within the substrate and mixed with the fluorescence and chemiluminescence emitted from the adjacent adsorbent regions. Therefore, it is possible to photoelectrically detect fluorescence or chemiluminescence and generate biochemical analysis data with excellent quantitativeness.

[0073] In a preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the light is transmitted through the substrate. Has the property of attenuating the energy of 1/5 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adjacent absorptive regions, 1/10 of the energy of light
It has the following property of damping.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adjacent absorptive regions, 1/50 the energy of light
It has the following property of damping.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adjacent absorptive regions, 1/10 of the energy of light
It has the property of being attenuated to 0 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adjacent absorptive regions, 1/50 the energy of light
It has the property of being attenuated to 0 or less.

In a further preferred aspect of the present invention, when the substrate of the biochemical analysis unit transmits light through the substrate by a distance equal to the distance between the adjacent absorptive regions, 1/10 of the energy of light
It has the property of being attenuated to 00 or less.

In the present invention, the plurality of absorptive regions of the biochemical analysis unit are formed by containing a receptor or a ligand in the absorptive material filled in the plurality of holes formed in the substrate. Alternatively, when the plurality of absorptive regions of the biochemical analysis unit are formed by containing the receptor or the ligand in the absorptive substrate in the plurality of through holes formed in the substrate, the biochemical analysis is performed. The material for forming the substrate of the analysis unit preferably has a property of attenuating radiation and / or light, but is not particularly limited, and may be an inorganic compound material or an organic compound material. Can also be used,
Metal material, ceramic material or plastic material
Preferably used.

In the present invention, a plurality of absorptive regions of the biochemical analysis unit are formed by containing a receptor or a ligand in the absorptive material filled in the plurality of holes formed in the substrate. Alternatively, when a plurality of absorptive regions of the biochemical analysis unit are formed by including a receptor or a ligand in the absorptive substrate in the plurality of through holes formed in the substrate, the biochemical analysis is performed. Examples of the inorganic compound material that can be preferably used to form the substrate of the unit for use include gold, silver,
Copper, zinc, aluminum, titanium, tantalum, chrome,
Metals such as iron, nickel, cobalt, lead, tin and selenium;
Alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; tungsten carbide, calcium carbonate, sulfuric acid. Inorganic salts such as calcium, hydroxyapatite and gallium arsenide can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a plurality of absorptive regions of the biochemical analysis unit are formed by containing a receptor or a ligand in an absorptive material filled in a plurality of holes formed in the substrate. Alternatively, when a plurality of absorptive regions of the biochemical analysis unit are formed by including a receptor or a ligand in the absorptive substrate in the plurality of through holes formed in the substrate, the biochemical analysis is performed. As the organic compound material that can be used to form the substrate of the unit for use, a polymer compound is preferably used, and a polymer compound that can be preferably used is, for example, a polyolefin such as polyethylene or polypropylene; polymethylmethacrylate. , Butyl acrylate /
Acrylic resins such as methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylon 6 Polyamide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose Polysaccharides such as cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; ; Chitosan; lacquer; gelatin, collagen, copolymers of polyamides and these high molecular compounds, such as keratin, and the like.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the plurality of absorptive regions of the biochemical analysis unit are receptive to the absorptive material filled in the plurality of holes formed in the substrate. Alternatively, when it is formed by containing a ligand, or when the plurality of absorptive regions of the biochemical analysis unit are formed in a plurality of through holes formed on the substrate, the absorptive substrate is made to contain a receptor or a ligand. When formed, the substrate of the biochemical analysis unit is preferably made of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more, and a specific gravity of 1.5 g / cm ³ or more. , 23 g / cm ³ or less of the compound material or the composite material is particularly preferable.

In general, the larger the light scattering and / or the light absorption, the higher the light attenuating ability. Therefore, the plurality of absorptive regions of the biochemical analysis unit are filled in the plurality of holes formed in the substrate. When the adsorbent material is formed by containing a receptor or a ligand in the adsorbent material, or when the plurality of absorptive regions of the biochemical analysis unit are formed in the plurality of through holes formed in the substrate In the case where the substrate of the biochemical analysis unit is formed by containing a receptor or a ligand, it is preferable that the absorbance per 1 cm of thickness is 0.3 or more, and the thickness of 1 c
More preferably, the absorbance per m is 1 or more.
Here, the absorbance is calculated by calculating the A / T by placing an integrating sphere immediately after the plate having a thickness of Tcm and measuring the transmitted light amount A at the wavelength of the probe light or the emission light used for the measurement with a spectrophotometer. By being asked. In order to improve the light attenuating ability, a light scatterer or a light absorber may be contained in the substrate of the biochemical analysis unit.
Fine particles of a material different from the material forming the substrate of the biochemical analysis unit are used as the light scatterer, and pigments or dyes are used as the light absorber.

In another preferred embodiment of the present invention, the biochemical analysis unit comprises an absorptive substrate,
The plurality of absorptive regions are formed at different positions of the absorptive substrate by dropping a solution containing a receptor or a ligand according to a regular pattern.

In the present invention, a porous material or a fibrous material is preferably used as the absorptive material for forming the absorptive region or the absorptive substrate of the biochemical analysis unit. The absorptive region or the absorptive substrate can be formed by using the porous material and the fiber material together.

In the present invention, the porous material used to form the adsorptive region of the biochemical analysis unit or the adsorptive substrate may be either an organic material or an inorganic material, or an organic / inorganic composite. .

In the present invention, the organic porous material used to form the absorptive region or the absorptive substrate of the biochemical analysis unit is not particularly limited, but is a carbon material such as activated carbon or a membrane. A material capable of forming a filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6, nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof. .

In the present invention, the inorganic porous material used for forming the absorptive region or the absorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, platinum, Examples thereof include metals such as gold, iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fibrous material used to form the adsorptive region or the adsorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, nylon 6 or nylon. Nylons such as 6,6 and nylon 4,10, nitrocellulose,
Examples thereof include cellulose derivatives such as cellulose acetate and cellulose butyrate.

In the present invention, the absorptive region of the biochemical analysis unit is subjected to electrolytic treatment, plasma treatment, oxidation treatment such as arc discharge; primer treatment using a silane coupling agent, titanium coupling agent, etc .; surfactant. It can also be formed by surface treatment such as treatment.

The object of the present invention is also to provide a reaction container provided with a biochemical analysis unit holding section for holding a biochemical analysis unit in which a plurality of absorptive regions containing a receptor or a ligand are formed apart from each other. , The reaction vessel, on one side of the biochemical analysis unit holding portion,
With a first electrode group consisting of a plurality of linear electrodes, on the other side of the biochemical analysis unit holding portion,
A second electrode group consisting of a plurality of line-shaped electrodes, the plurality of line-shaped electrodes constituting the first electrode group, and the plurality of line-shaped electrodes constituting the second electrode group,
This is achieved by a reactor characterized by being arranged so as to intersect at positions corresponding to the plurality of absorptive regions of the biochemical analysis unit held by the biochemical analysis unit holding portion.

According to the present invention, the reactor is provided with a biochemical analysis unit holding section for holding a biochemical analysis unit in which a plurality of absorptive regions containing a receptor or a ligand are formed separately from each other. With a container, the reaction container, on one side of the unit holding unit for biochemical analysis, with a first electrode group consisting of a plurality of linear electrodes, on the other side of the unit holding unit for biochemical analysis, A biochemical analysis unit including a second electrode group including a plurality of line-shaped electrodes, the plurality of line-shaped electrodes forming the first electrode group, and the plurality of line-shaped electrodes forming the second electrode group. Since the biochemical analysis unit held by the holding unit is arranged so as to intersect at a position corresponding to the plurality of absorptive regions, for example, the plurality of line-shaped electrodes constituting the first electrode group Two or more In addition, a positive or negative voltage is applied to the non-adjacent line electrodes, and a negative or negative voltage is applied to two or more non-adjacent line electrodes of the plurality of line electrodes forming the second electrode group. A positive voltage is applied, and a negative or positive voltage is applied to two or more non-adjacent line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes forming the first electrode group. In addition, by applying a positive or negative voltage to two or more line-shaped electrodes that are different from the two or more line-shaped electrodes that are different from the two or more line-shaped electrodes that form the second electrode group, The ligand or receptor contained in the reaction solution housed in the reaction container is transferred from different directions to a plurality of units formed in the biochemical analysis unit. It can be forcibly moved across the adhesive region, so that only the ligand or receptor contained in the reaction solution can be transferred from different directions to the multiple adsorptive regions formed in the biochemical analysis unit. Since it can be forcibly moved across, the ligand or receptor contained in the reaction solution is simply moved by convection or diffusion and contained in the adsorptive region of the biochemical analysis unit. The migration rate of the ligand or the receptor within the adsorptive region of the biochemical analysis unit can be significantly increased as compared with the case where the receptor or the ligand is allowed to react with each other, and therefore, the receptor-ligand association reaction It is possible to greatly improve the reaction rate, and further, to improve the adsorption area of the biochemical analysis unit. It is possible to significantly increase the probability that the receptor or ligand contained in the deep portion and the ligand or receptor contained in the reaction solution associate with each other. Receptor or ligand contained in the adsorptive region,
It becomes possible to associate and react with the ligand or the receptor contained in the reaction solution.

Further, according to the present invention, for example, a plus or minus voltage is applied to two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the first electrode group, and A negative or positive voltage is applied to two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the second electrode group, and Two or more lines of a plurality of line-shaped electrodes that form a second electrode group while applying a negative or positive voltage to two or more line-shaped electrodes that are not adjacent to each other and are different from the two or more line-shaped electrodes By applying a positive or negative voltage to two or more line-shaped electrodes that are not adjacent to each other and are different from the circular electrodes, the reaction solution contained in the reaction vessel It is possible to forcibly move the rare ligand or receptor from different directions so as to cross the multiple absorptive regions formed in the biochemical analysis unit, and the reaction solution is stirred. Similarly, the ligands or receptors can be uniformly mixed in the reaction solution, and therefore, the ligands or receptors contained in the reaction solution and the multiple adsorption regions of the biochemical analysis unit are contained. Since it is possible to associate with the receptor or the ligand with almost equal probability, the receptor or the ligand contained in the plurality of adsorptive regions of the biochemical analysis unit and the reaction solution can be contained in the reaction solution as desired. The associated ligand or receptor is allowed to associate and react.

Further, the reaction solution is circulated in the reaction vessel through the solution circulation pipe using a pump or the like,
When flowing across a plurality of absorptive regions formed in the biochemical analysis unit, it is necessary to fill the reaction vessel and the solution circulation pipeline with the reaction solution, and the concentration of the reaction components is high. Although it is difficult to use the reaction solution, according to the present invention, for example, simply, two or more non-adjacent linear electrodes of a plurality of linear electrodes forming the first electrode group, The two or more line-shaped electrodes that are not adjacent to each other of the plurality of line-shaped electrodes that form the electrode group are crossed over the plurality of absorptive regions of the biochemical analysis unit by simply applying opposite voltages.
Since it is possible to forcibly move the ligand or receptor contained in the reaction solution, the reaction vessel with a small volume is filled with the reaction solution having a high concentration of reaction components, and a plurality of biochemical analysis units are used. The ligand or receptor contained in the reaction solution can be allowed to undergo an association reaction with the receptor or ligand contained in the adsorptive region of A, and thus the reaction rate of the receptor-ligand association reaction can be significantly increased. become.

[0095]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit used in a receptor-ligand association reaction method according to a preferred embodiment of the present invention.

As shown in FIG. 1, the biochemical analysis unit 1 according to this embodiment includes a substrate 2 formed of aluminum and having a large number of substantially circular through holes 3 formed therein at a high density. Nylon 6 is filled inside the large number of through holes 3 to form a large number of dot-shaped absorptive regions 4
Are formed.

Although not shown exactly in FIG. 1, in the present embodiment, the substantially circular through holes 3 having a size of about 0.01 mm 2 are regularly arranged in a matrix of 120 columns × 160 rows. Is formed on the substrate 2,
Therefore, a total of 19200 absorptive regions 4 are formed. The absorptive region 4 is formed by filling a large number of through holes 3 with nylon 6 so that the surface thereof is located at the same height as the surface of the substrate 2.

In the biochemical analysis, in a large number of absorptive regions 4 regularly formed in the biochemical analysis unit 1, for example, as a specific binding substance, a plurality of different cDNAs whose base sequences are known are different from each other. Are dropped using a spotting device and adsorbed in the absorptive region 4.

FIG. 2 is a schematic front view of the spotting device.

As shown in FIG. 2, the spotting device is equipped with an injector 5 and a CCD camera 6 for injecting a solution of a specific binding substance toward the surface of the biochemical analysis unit 1. Injector 5
While observing the tip of the injector and the absorptive region 4 of the biochemical analysis unit 1 on which the specific binding substance should be dropped, the tip of the injector 5 and the center of the absorptive region 4 on which the specific binding substance should be dropped. When and match, from the injector 5,
It is configured such that a plurality of specific binding substances such as cDNAs having different known base sequences are dropped, and in the many dot-shaped absorptive regions 4 of the biochemical analysis unit 1,
The specific binding substance is guaranteed to be able to be dripped accurately.

Then, in this manner, the specific binding substance such as cDNA adsorbed on the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is separated from the substance derived from the living body labeled with the labeling substance. , Selectively, hybridized.

FIG. 3 is a schematic vertical sectional view of a reactor used in the method of receptor-ligand association reaction according to a preferred embodiment of the present invention.

As shown in FIG. 3, the reactor according to this embodiment comprises a lid member 10 and a casing 11.
A reaction container 13 having a biochemical analysis unit holding member 12 for holding the biochemical analysis unit 1 is provided.

In this embodiment, the lid member 10 and the casing 11 of the reaction vessel 13 are made of a non-conductive material, and the reaction vessel 13 is for biochemical analysis held by the biochemical analysis unit holding member 12. Unit 1
It has a small volume on both sides of which a reaction solution such as a hybridization reaction solution of a predetermined amount or less can be stored.

As shown in FIG. 3, the reaction container 13 includes a plurality of linear electrodes on both sides of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. One electrode group 14 and a second electrode group 15 including a large number of line-shaped electrodes are provided.

FIG. 4 is a schematic plan view of the first electrode group 14 and the second electrode group 15.

As shown in FIG. 4, when the biochemical analysis unit 1 is held in the reaction vessel 13 by the biochemical analysis unit holding member 12, it is positioned above the biochemical analysis unit 1. The first electrode group 14 is composed of a large number of linear electrodes 14a, 14b, 14c, 14d ,.
The second electrode group 15 having a length of 4 m and located below the biochemical analysis unit 1 includes a large number of line-shaped electrodes 15a and 15a.
b, 15c, 15d, ... 15n.

As shown in FIG. 4, the first electrode group 14
The line-shaped electrodes 14a, 14b, 14c, 14
14m, and the line-shaped electrodes 15a, 15b, 15c, 15d, ... 15n forming the second electrode group 15.
Are arranged in the reaction vessel 13 so as to be orthogonal to each other.

Although not shown accurately in FIG. 4, the line-shaped electrodes 14a, 14b, 1 constituting the first electrode group 14 are formed.
4c, 14d, ... 14m, and the linear electrodes 15a, 15b, 15c, 15d, ...
The intersection with 15n in a plan view has the same pattern as many absorptive regions 4 formed in the biochemical analysis unit 1, and therefore m = 120, n = 160.
Thus, a total of 19,200 intersections are formed.

Further, the biochemical analysis unit holding member 12 for holding the biochemical analysis unit 1 is the first electrode group 1
4 line electrodes 14a, 14b, 14c, 1
4d, ..., 14m, and the line electrodes 15a, 15b, 15 forming the second electrode group 15
It is set to an intermediate potential between the potentials to be given to c, 15d, ...

In the reactor according to the present embodiment configured as described above, the specific binding substance contained in the large number of absorptive regions 4 of the biochemical analysis unit 1 is labeled with the labeling substance as follows. The substance derived from the living body labeled by and contained in the hybridization reaction solution is selectively hybridized.

First, the biochemical analysis unit 1 in which the lid member 10 of the reaction container 13 is opened by the user and the specific binding substance is fixed to a large number of adsorptive regions 4 is set in the biochemistry of the reaction container 13. It is attached to the analysis unit holding member 12.

Next, a hybridization reaction solution is prepared and housed in the reaction container 13.

When a specific binding substance such as cDNA is selectively labeled with a radiolabeling substance, a hybridization reaction solution containing a substance derived from a living body which is a probe labeled with the radiolabeling substance is prepared, It is accommodated in the reaction container 13.

On the other hand, with a fluorescent substance such as a fluorescent dye,
In the case of selectively labeling a specific binding substance such as cDNA, a hybridization reaction solution containing a substance of biological origin which is a probe labeled with a fluorescent substance such as a fluorescent dye is prepared and placed in the reaction container 13. Be accommodated.

[0117] The cDNA may be labeled with a labeling enzyme that produces chemiluminescence by contacting it with a chemiluminescent substrate.
In the case of selectively labeling a specific binding substance such as, a hybridization reaction solution containing a substance of biological origin labeled with a hapten such as digoxigenin is prepared and housed in the reaction container 13.

Two or more of the living body-derived substances out of the living body-derived substances labeled with a radioactive labeling substance, the living body-derived substances labeled with a fluorescent substance such as a fluorescent dye, and the hapten-labeled substances such as digoxigenin It is also possible to prepare a reaction solution containing the substance of, and in this embodiment, a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance, and a hapten such as digoxigenin. A hybridization reaction solution containing the substance derived from the living body is prepared and housed in the reaction container 13.

In this embodiment, since the volume of the reaction container 13 is small, it is possible to prepare the hybridization reaction solution having a high concentration of the substance derived from the living body to fill the reaction container 13.

When the inside of the reaction container 13 is filled with the hybridization reaction solution, the lid member 10 of the reaction container 13 is closed, and a large number of line-shaped electrodes 14a, 14b, 14c, ... ... of the odd-numbered linear electrodes 14a, 14c, ... Of 14m,
Negative voltage is supplied and the second electrode group 1
5, a large number of line-shaped electrodes 15a, 15b, 15
A positive voltage is supplied to the odd-numbered linear electrodes 15a, 15c, ... Of c, 15d ,.

As a result, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction container 13 is an odd-numbered linear electrode forming the second electrode group 15 to which a positive voltage is applied. 15a, 15c, ...
Across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and is forcibly moved from the upper side to the lower side in FIG. The substance of biological origin contained in the hybridization reaction solution selectively hybridizes to the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.

Next, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, even-numbered line-shaped electrodes 14b, 14d ,.
Is supplied with a positive voltage, and a large number of line-shaped electrodes 15a, 15b, 1 forming the second electrode group 15 are provided.
A negative voltage is supplied to the odd-numbered linear electrodes 15a, 15c, ... Of 5c, 15d ,.

As a result, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction vessel 13 is an even-numbered linear electrode constituting the first electrode group 14 to which a positive voltage is applied. 14b, 14d, ...
Across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and is forcibly moved from the lower side to the upper side in FIG. The substance of biological origin contained in the hybridization reaction solution selectively hybridizes to the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.

At the same time, the biochemical analysis unit 1 is not hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.
The substance derived from a living body adsorbed on the large number of absorptive regions 4 formed on the substrate 2 is also an even-numbered linear electrode 14b constituting the first electrode group 14 to which a positive voltage is applied,
14d, ... attracted to biochemical analysis unit 1
From the absorptive region 4 of the above, it is forcibly peeled off and removed.

Further, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, the even-numbered line-shaped electrodes 14b, 14d ,.
Is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15b, which constitute the second electrode group 15,
A positive voltage is supplied to the even-numbered linear electrodes 15b, 15d, ... Of 15c, 15d ,.

As a result, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction vessel 13 is an even-numbered linear electrode constituting the second electrode group 15 to which a positive voltage is applied. 15b, 15d, ...
Across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and is forcibly moved from the upper side to the lower side in FIG. The substance of biological origin contained in the hybridization reaction solution selectively hybridizes to the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.

At the same time, the biochemical analysis unit 1 was not hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.
The substance derived from a living body adsorbed on the large number of absorptive regions 4 formed on the substrate 2 is also an even-numbered linear electrode 15b constituting the second electrode group 15 to which a positive voltage is applied,
Unit 1 for biochemical analysis attracted to 15d, ...
From the absorptive region 4 of the above, it is forcibly peeled off and removed.

Next, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, the odd-numbered line-shaped electrodes 14a, 14c ,.
Is supplied with a positive voltage, and a large number of line-shaped electrodes 15a, 15b, 1 forming the second electrode group 15 are provided.
A negative voltage is supplied to the even-numbered linear electrodes 15b, 15d, ... Of 5c, 15d ,.

As a result, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction vessel 13 is an odd-numbered linear electrode constituting the first electrode group 14 to which a positive voltage is applied. 14a, 14c, ...
Across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and is forcibly moved from the lower side to the upper side in FIG. The substance of biological origin contained in the hybridization reaction solution selectively hybridizes to the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.

At the same time, the biochemical analysis unit 1 is not hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.
The substance derived from the living body adsorbed to the large number of absorptive regions 4 formed on the substrate 2 is also an odd-numbered linear electrode 14a that constitutes the first electrode group 14 to which a positive voltage is applied,
Unit 1 for biochemical analysis attracted to 14c ...
From the absorptive region 4 of the above, it is forcibly peeled off and removed.

Further, of the many line electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, the odd-numbered line electrodes 14a, 14c ,.
Is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15b, which constitute the second electrode group 15,
A positive voltage is supplied to the odd-numbered linear electrodes 15a, 15c, ... Of 15c, 15d ,.

As a result, the substance of biological origin contained in the hybridization reaction solution filled in the reaction vessel 13 is an odd-numbered linear electrode forming the second electrode group 15 to which a positive voltage is applied. 15a, 15c, ...
Across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and is forcibly moved from the upper side to the lower side in FIG. The substance of biological origin contained in the hybridization reaction solution selectively hybridizes to the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.

At the same time, the biochemical analysis unit 1 is not hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1.
The substance derived from the living body adsorbed to the large number of absorptive regions 4 formed on the substrate 2 is also an odd-numbered linear electrode 15a constituting the second electrode group 15 to which a positive voltage is applied,
Unit 1 for biochemical analysis attracted to 15c, ...
From the absorptive region 4 of the above, it is forcibly peeled off and removed.

The same operation is repeated for a predetermined time.

As described above, in this embodiment, a large number of line-shaped electrodes 14a, 1a constituting the first electrode group 14 are formed.
14b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a constituting the second electrode group 15.
15b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the living body contained in the hybridization reaction solution filled in the reaction vessel 13 is selected. The derived substance is attracted to a predetermined linear electrode to which a positive voltage is applied, and is forcibly passed across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Therefore, the substance derived from the living body contained in the hybridization reaction solution can be easily transferred by convection or diffusion, and compared with the case where the substance is hybridized with the specific binding substance. It is possible to significantly increase the moving speed of the substance derived from the living body in the adsorptive region 4 of the chemical analysis unit 1; The kinetics of a hybridization reaction it becomes possible to greatly improve, furthermore,
It is possible to greatly increase the probability that a substance derived from a living body encounters a specific binding substance contained in a deep portion of the adsorptive region 4, and therefore, as desired, a large number of biochemical analysis unit 1 It becomes possible to hybridize the substance of biological origin contained in the hybridization reaction solution with the specific binding substance fixed to the adsorptive region 4.

In the present embodiment, a large number of absorptive regions 4 formed on the substrate 2 in the biochemical analysis unit 1 are used.
For each of the plurality of line-shaped electrodes 1 that form the first electrode group 14 without providing a pair of electrodes.
A large number of line-shaped electrodes 15a, 15b, 15c forming a second electrode group 15 by selecting a predetermined line-shaped electrode from among 4a, 14b, 14c, ... 14m and applying a positive or negative voltage. , 15d, ... out of 15n
By selecting a predetermined linear electrode and applying a negative or positive voltage, the bio-derived substance contained in the hybridization reaction solution filled in the reaction vessel 13 can be biochemically reacted as desired. Biochemical analysis unit 1 held by analysis unit holding member 12
Since it can be forcibly moved across a large number of adsorptive regions 4, it is possible to greatly improve the reaction rate of the hybridization reaction by using a reactor having a simple structure and a simple reaction operation. Furthermore, it is possible to significantly increase the probability that a substance derived from a living body encounters a specific binding substance contained in a deep portion of the adsorptive region 4, and therefore, as desired, for biochemical analysis. It becomes possible to hybridize the substance of biological origin contained in the hybridization reaction solution with the specific binding substance immobilized on the numerous absorptive regions 4 of the unit 1.

Further, the hybridization reaction solution is circulated in the reaction vessel 13 via the solution circulation pipe using a pump or the like, and a large number of adsorptions formed on the substrate 2 of the biochemical analysis unit 1 are adsorbed. In the case where the reaction container 13 and the solution circulation pipe are to be flowed across the property region 4, it is necessary to fill the inside of the reaction container 13 and the solution circulation conduit with the hybridization reaction solution, and a hybridization reaction solution having a high concentration of a substance derived from a living body is used. Difficult to do,
In the present embodiment, simply a large number of line-shaped electrodes 14a, 14b, 14c, ...
A predetermined line-shaped electrode is selected from 14 m, and a positive or negative voltage is applied to the large number of line-shaped electrodes 15 a, 15 b, 15 c, 1 to form the second electrode group 15.
5d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction vessel 13 , Is attracted to a predetermined linear electrode to which a positive voltage is applied, and is forcibly moved across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Therefore, the reaction vessel 13 having a small volume was filled with the hybridization reaction solution having a high concentration of the substance derived from the living body and fixed to the large number of absorptive regions 4 of the biochemical analysis unit 1. The specific binding substance can be hybridized with the substance of biological origin contained in the hybridization reaction solution, and thus It is possible to greatly increase the reaction rate of hybridization reactions.

Further, in this embodiment, simply, the large number of line-shaped electrodes 14a, 1a constituting the first electrode group 14 are used.
14b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a constituting the second electrode group 15.
15n among 15b, 15c, 15d, ..., 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the uniqueness contained in the absorptive region 4 of the biochemical analysis unit 1 is selected. Unit 1 for biochemical analysis, even though it is not hybridized to the chemically bound substance
The substance of biological origin adsorbed on the large number of absorptive regions 4 formed on the substrate 2 is attracted to the linear electrodes to which a positive voltage is applied, and the absorptive regions 4 of the biochemical analysis unit 1 are attracted. Since it can be peeled off from the biochemical analysis unit 1, a substance derived from a living body that has not hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 is
It is possible to prevent deterioration of quantitativeness due to adsorption to the absorptive region 4 of the biochemical analysis unit 1 and generate biochemical analysis data having excellent quantitativeness. Become.

In this way, a large number of line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14 are formed.
Among them, a predetermined line-shaped electrode is selected and a positive or negative voltage is applied thereto, and a large number of line-shaped electrodes 15a, 15b, 15c, 15 forming the second electrode group 15 are selected.
The operation of selecting a predetermined linear electrode among d, ..., 15n and applying a negative or positive voltage is repeated, and the hybridization reaction is completed when a predetermined time elapses.

When the hybridization reaction is completed, the hybridization reaction solution is transferred to the reaction container 13
Emitted from.

When the hybridization reaction solution is discharged from the reaction container 13, the washing solution is removed from the reaction container 13.
A large number of absorptive regions 4 housed inside and formed on the substrate 2 of the biochemical analysis unit 1 are washed with a washing solution.

When the cleaning operation is completed after a lapse of a predetermined time, the cleaning solution is discharged from the reaction container 13.

As described above, the radiation data of the radioactive labeling substance as the labeling substance and the fluorescence data of the fluorescent substance such as the fluorescent dye are provided in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Is recorded. The fluorescence data recorded in the absorptive region 4 of the biochemical analysis unit 1 is read by a scanner described later to generate biochemical analysis data.

On the other hand, the radiation data of the radiolabeled substance is transferred to the stimulable phosphor sheet, and the radiation data transferred to the stimulable phosphor sheet is read by the scanner described later for biochemical analysis. Data is generated.

On the other hand, in order to record chemiluminescence data in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, further chemiluminescence is generated by bringing the chemiluminescence substrate into contact. An antibody solution containing an antibody against a hapten such as digoxigenin labeled with an enzyme is prepared, housed in a reaction vessel 13, and contained in a large number of absorptive regions 4 formed on a substrate 2 of the biochemical analysis unit 1. The specific binding substance, which is selectively, to a hapten such as digoxigenin that labels the substance of biological origin that has been hybridized, by an antigen-antibody reaction,
An antibody to the hapten labeled with an enzyme having a property of causing chemiluminescence by contacting with a chemiluminescent substrate is bound.

That is, first, an antibody solution containing an antibody against a hapten such as digoxigenin labeled with an enzyme that produces chemiluminescence by contacting with a chemiluminescent substrate is prepared and filled in the reaction vessel 13.

When the reaction container 13 is filled with the antibody solution, the lid member 10 of the reaction container 13 is closed and a large number of line-shaped electrodes 14a constituting the first electrode group 14 are formed.
14b, 14c, ... 14m, a negative voltage is supplied to the odd-numbered line-shaped electrodes 14a, 14c, ... And a large number of line-shaped electrodes 15a, 15b forming the second electrode group 15. , 15c, 15d, …… 15
Of n, odd-numbered linear electrodes 15a, 15c, ...
Is supplied with a positive voltage.

As a result, the antibodies contained in the antibody solution filled in the reaction vessel 13 are the odd-numbered linear electrodes 1 forming the second electrode group 15 to which the positive voltage is applied.
5a, 15c, ..., across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, in FIG. The specific binding substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 after being forcibly moved is selectively labeled with a substance derived from the living body that has been hybridized. The hapten such as digoxigenin and the antibody against the hapten labeled with the enzyme having the property of causing chemiluminescence by contacting with the chemiluminescent substrate are bound by the antigen-antibody reaction.

Next, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, even-numbered line-shaped electrodes 14b, 14d ,.
Is supplied with a positive voltage, and a large number of line-shaped electrodes 15a, 15b, 1 forming the second electrode group 15 are provided.
A negative voltage is supplied to the odd-numbered linear electrodes 15a, 15c, ... Of 5c, 15d ,.

As a result, the antibodies contained in the antibody solution filled in the reaction vessel 13 are made to form the even-numbered linear electrodes 1 forming the first electrode group 14 to which a positive voltage is applied.
4b, 14d, ..., across the many absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, in FIG. 3, from the bottom to the top, The specific binding substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 after being forcibly moved is selectively labeled with a substance derived from the living body that has been hybridized. The hapten such as digoxigenin and the antibody against the hapten labeled with the enzyme having the property of causing chemiluminescence by contacting with the chemiluminescent substrate are bound by the antigen-antibody reaction.

At the same time, a specific binding substance contained in the absorptive region 4 of the biochemical analysis unit 1 is selectively attached to a hapten such as digoxigenin which is labeled with a substance derived from a living body which is hybridized. By the antigen-antibody reaction,
Although not bound, the antibody adsorbed to the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 has the first electrode group 14 to which a positive voltage is applied. Of the even-numbered linear electrodes 14b, 14
, and is forcibly peeled off and removed from the absorptive region 4 of the biochemical analysis unit 1.

Further, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, even-numbered line-shaped electrodes 14b, 14d ,.
Is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15b, which constitute the second electrode group 15,
A positive voltage is supplied to the even-numbered linear electrodes 15b, 15d, ... Of 15c, 15d ,.

As a result, the antibodies contained in the antibody solution filled in the reaction container 13 are made to form the even-numbered linear electrodes 1 forming the second electrode group 15 to which the positive voltage is applied.
5b, 15d, ..., across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, in FIG. The specific binding substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 after being forcibly moved is selectively labeled with a substance derived from the living body that has been hybridized. The hapten such as digoxigenin and the antibody against the hapten labeled with the enzyme having the property of causing chemiluminescence by contacting with the chemiluminescent substrate are bound by the antigen-antibody reaction.

At the same time, a specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 is selectively hapten such as digoxigenin labeled with a hybridized substance of biological origin. By the antigen-antibody reaction,
Although not bound, the antibody adsorbed to the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 has a second electrode group 15 to which a positive voltage is applied. Of the even-numbered linear electrodes 15b, 15
, and is forcibly peeled off and removed from the absorptive region 4 of the biochemical analysis unit 1.

Next, of the many line-shaped electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, the odd-numbered line-shaped electrodes 14a, 14c ,.
Is supplied with a positive voltage, and a large number of line-shaped electrodes 15a, 15b, 1 forming the second electrode group 15 are provided.
A negative voltage is supplied to the even-numbered linear electrodes 15b, 15d, ... Of 5c, 15d ,.

As a result, the antibodies contained in the antibody solution filled in the reaction vessel 13 are changed to the odd-numbered linear electrodes 1 forming the first electrode group 14 to which the positive voltage is applied.
4a, 14c, ..., across the many absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, in FIG. 3, from the bottom to the top, The specific binding substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 after being forcibly moved is selectively labeled with a substance derived from the living body that has been hybridized. The hapten such as digoxigenin and the antibody against the hapten labeled with the enzyme having the property of causing chemiluminescence by contacting with the chemiluminescent substrate are bound by the antigen-antibody reaction.

At the same time, the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 is selectively hapten such as digoxigenin, which is labeled with the substance derived from the living body. By the antigen-antibody reaction,
Although not bound, the antibody adsorbed to the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 has the first electrode group 14 to which a positive voltage is applied. Of the odd-numbered linear electrodes 14a, 14
, and is forcibly peeled off and removed from the absorptive region 4 of the biochemical analysis unit 1.

Further, of the large number of line electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14, the odd-numbered line electrodes 14a, 14c ,.
Is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15b, which constitute the second electrode group 15,
A positive voltage is supplied to the odd-numbered linear electrodes 15a, 15c, ... Of 15c, 15d ,.

As a result, the antibodies contained in the antibody solution filled in the reaction container 13 are changed to the odd-numbered linear electrodes 1 forming the second electrode group 15 to which the positive voltage is applied.
5a, 15c, ..., across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, in FIG. The specific binding substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 after being forcibly moved is selectively labeled with a substance derived from the living body that has been hybridized. The hapten such as digoxigenin and the antibody against the hapten labeled with the enzyme having the property of causing chemiluminescence by contacting with the chemiluminescent substrate are bound by the antigen-antibody reaction.

At the same time, the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 is selectively hapten such as digoxigenin labeled with the hybridized substance of biological origin. By the antigen-antibody reaction,
Although not bound, the antibody adsorbed to the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 has a second electrode group 15 to which a positive voltage is applied. Of the odd-numbered linear electrodes 15a, 15
, and is forcibly peeled off and removed from the absorptive region 4 of the biochemical analysis unit 1.

The same operation is repeated for a predetermined time.

As described above, in this embodiment, a large number of line-shaped electrodes 14a, 1a constituting the first electrode group 14 are formed.
14b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a constituting the second electrode group 15.
15b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the antibody contained in the antibody solution filled in the reaction container 13 The biochemical analysis unit 1 held by the biochemical analysis unit holding member 12 by being attracted to a predetermined linear electrode to which a positive voltage is applied.
Since it is possible to forcibly move it across a large number of absorptive regions 4 of the antibody, the antibody contained in the antibody solution can be moved by convection or diffusion to bind to the antigen. Thus, the migration rate of the antibody in the adsorptive region 4 of the biochemical analysis unit 1 can be significantly increased, and thus the reaction rate of the antigen-antibody reaction can be significantly improved, and further, The antibody against the hapten contained in the antibody solution selectively meets the specific binding substance contained in the deep portion of the adsorptive region 4 with the hapten labeling the hybridized substance of biological origin. The probability can be greatly increased, and therefore the antibody to the hapten contained in the antibody solution and the adsorbable region 4 of the biochemical analysis unit 1 can be contained as desired. The specific binding substances, optionally,
The hapten labeling the hybridized substance derived from the living body can be bound by the antigen-antibody reaction.

Further, in this embodiment, a large number of absorptive regions 4 formed on the substrate 2 in the biochemical analysis unit 1 are used.
For each of the plurality of line-shaped electrodes 1 that form the first electrode group 14 without providing a pair of electrodes.
A large number of line-shaped electrodes 15a, 15b, 15c forming a second electrode group 15 by selecting a predetermined line-shaped electrode from among 4a, 14b, 14c, ... 14m and applying a positive or negative voltage. , 15d, ... out of 15n
By selecting a predetermined linear electrode and applying a negative or positive voltage, the antibody contained in the antibody solution filled in the reaction vessel 13 can be retained as desired in a unit holding member for biochemical analysis. Across a number of absorptive regions 4 of the biochemical analysis unit 1 held by 12,
Since it can be forcibly moved, it is possible to greatly improve the reaction rate of the antigen-antibody reaction by using a reactor with a simple structure and a simple reaction operation. The antibody against the existing hapten significantly increases the probability that the specific binding substance contained in the deep part of the adsorptive region 4 selectively and selectively encounters the hapten labeling the hybridized substance of biological origin. Therefore, as desired, the antibody to the hapten contained in the antibody solution and the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 can be selectively hybridized. The hapten labeling the substance derived from the living body can be bound by an antigen-antibody reaction.

Further, the antibody solution is circulated in the reaction vessel 13 through the solution circulation pipe using a pump or the like, and a large number of absorptive regions formed on the substrate 2 of the biochemical analysis unit 1 are circulated. In the case of flowing across 4 the reaction container 13 and the solution circulation conduit must be filled with the antibody solution, and it is difficult to use the antibody solution having a high antibody concentration. In, simply
A large number of linear electrodes 14 forming the first electrode group 14
a. 14b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied to the multiple line-shaped electrodes 1 forming the second electrode group 15.
Of the 5a, 15b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the antibodies contained in the antibody solution filled in the reaction container 13 are selected. Is attracted to a predetermined linear electrode to which a positive voltage is applied, and is forcibly moved across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Therefore, the inside of the reaction container 13 having a small volume is filled with the antibody solution having a high antibody concentration and is contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. The specific binding substance that has been selectively converted into a chemiluminescent substrate by selectively contacting a hapten such as digoxigenin that labels the hybridized substance of biological origin with a chemiluminescent substrate. An antibody to the hapten labeled with an enzyme having a property to cause light emission, by an antigen-antibody reaction, can be attached, it is possible to greatly increase the reaction rate of the antigen-antibody reaction.

Further, in this embodiment, simply, a large number of line-shaped electrodes 14a, 1a constituting the first electrode group 14 are used.
14b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a constituting the second electrode group 15.
15n among 15b, 15c, 15d, ..., 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the uniqueness contained in the absorptive region 4 of the biochemical analysis unit 1 is selected. To a hapten such as digoxigenin, which selectively labels the hybridized substance derived from the living body, by an antigen-antibody reaction,
The antibodies, which are not bound to each other, are adsorbed to a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 and are attracted to the linear electrodes to which a positive voltage is applied. Since it can be separated from the adsorptive region 4 of the biochemical analysis unit 1, it is selectively hybridized with the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1. Quantitativeness due to the fact that an antibody that is not bound to a hapten such as digoxigenin that labels a substance derived from a living body by an antigen-antibody reaction is adsorbed to the absorptive region 4 of the biochemical analysis unit 1. It is possible to prevent the deterioration of the value and generate the biochemical analysis data with excellent quantification.

Thus, a large number of linear electrodes 14a, 14b, 14c, ... 14m forming the first electrode group 14 are formed.
Among them, a predetermined line-shaped electrode is selected and a positive or negative voltage is applied thereto, and a large number of line-shaped electrodes 15a, 15b, 15c, 15 forming the second electrode group 15 are selected.
The operation of selecting a predetermined linear electrode from among d, ..., 15n and applying a negative or positive voltage is repeated, and when a predetermined time elapses, the antigen-antibody reaction is completed.

Thus, when a predetermined time has elapsed, the antigen-antibody reaction is completed, and the antibody solution is discharged from the reaction container 13.

When the antibody solution is discharged from the reaction vessel 13, the washing solution is accommodated in the reaction vessel 13 and a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are It is washed with a washing solution.

When the cleaning operation is completed after a lapse of a predetermined time, the cleaning solution is discharged from the reaction container 13.

As described above, chemiluminescence data is recorded in a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. The chemiluminescence data recorded in the absorptive region 4 of the biochemical analysis unit 1 is read by a cooling CCD camera of a data generation system described later, and biochemical analysis data is generated.

When the cleaning solution is completely discharged from the reaction container 13, the biochemical analysis unit holding member 12 allows the user to remove the biochemical analysis unit 1 held in the reaction container 13 from the reaction container 13. , Taken out.

FIG. 5 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 5, the stimulable phosphor sheet 20 according to this embodiment has a large number of substantially circular through holes 2.
2 includes a nickel-made support 21 formed regularly, and a stimulable phosphor is embedded in a large number of through holes 22 formed in the support 21 to form a large number of stimulable phosphor layers. The area 24 is formed in a dot shape.

The large number of through holes 22 are formed in the support 21 in the same pattern as the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and each stimulable phosphor layer region. 24 is formed to have the same size as the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1.

Therefore, although not accurately shown in FIG. 5, a substantially circular stimulable phosphor layer region 24 of 19200 having a size of about 0.01 mm 2 is formed on the substrate of the biochemical analysis unit 1. The matrix 21 is formed in a matrix on the support 21 of the stimulable phosphor sheet 20 by the same regular pattern as the large number of the absorptive regions 4 formed in 2.

Further, in this embodiment, the support 21
The stimulable phosphor is embedded in the through hole 22 formed in the support 21 and accumulated so that the surface of the stimulable phosphor layer region 24 and the surface of the stimulable phosphor layer region 24 are located at the same height. Fluorescent phosphor sheet 20 is formed.

FIG. 6 shows a large number of stimulable phosphor layers formed on the stimulable phosphor sheet 20 by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1. FIG. 6 is a schematic cross-sectional view showing a method of exposing a region 24.

As shown in FIG. 6, upon exposure,
The absorptive property so that the large number of absorptive regions 4 formed in the biochemical analysis unit 1 face the large number of stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20. The phosphor sheet 20 and the biochemical analysis unit 1 are superposed.

In this way, each of the stimulable phosphor layer regions 24 formed on the stimulable phosphor sheet 20 and the plurality of absorptive regions 4 formed on the biochemical analysis unit 1 over a predetermined period of time. By facing each other, the radioactive labeling substance contained in the absorptive region 4 causes the stimulable phosphor contained in the large number of stimulable phosphor layer regions 24 formed in the stimulable phosphor sheet 20 to be generated. Exposed.

At this time, an electron beam (β-ray) is emitted from the radiolabeled substance adsorbed in the absorptive region 4, but the absorptive region 4 of the biochemical analysis unit 1 has a property of attenuating radiation. Since the substrate 2 made of aluminum is formed so as to be separated from each other, the electron beam (β-ray) emitted from each absorptive region 4 is scattered in the substrate 2 of the biochemical analysis unit 1. And effectively prevent it from being mixed with the electron beam (β-ray) emitted from the adsorbing regions 4 adjacent to each other and entering the photostimulable phosphor layer region 24 facing the adsorbing regions 4 adjacent to each other. In addition, a large number of stimulable phosphor layer regions 24 of the stimulable phosphor sheet 20 are formed.
Is formed by embedding a stimulable phosphor in a plurality of through-holes 22 formed in a nickel support 21 having a property of attenuating radiation.
The electron beam (β-ray) emitted from the substrate is scattered inside the support 21 of the stimulable phosphor sheet 20, and is emitted to the stimulable phosphor layer region 24 adjacent to the opposing stimulable phosphor layer region 24. It becomes possible to effectively prevent the incident light, and therefore, the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive region 4 is radiated to oppose the adsorptive region 4. The electron beam (β-ray) emitted from the radioactive labeling substance contained in the absorptive region 4 can be selectively made incident on the exhaustive phosphor layer region 24, and is emitted from the adjacent absorptive region 4. It is possible to reliably prevent the photostimulable phosphor from being exposed by being incident on the photostimulable phosphor layer region 24 to be exposed by the electron beam (β-ray).

Thus, a large number of stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20 are
Radiation data of radiolabeled substances are recorded.

FIG. 7 shows the radiation data of the radio-labeled substance recorded in a large number of stimulable phosphor layer regions 24 formed on the stimulable phosphor sheet 20 and the biochemical analysis unit 1.
9 is a schematic perspective view of a scanner that reads fluorescence data recorded in a large number of absorptive regions 4 and generates biochemical analysis data. FIG. 8 is a schematic perspective view showing details of a scanner near a photomultiplier. Is.

The scanner shown in FIG. 7 has a large number of stimulable phosphor layer regions 24 formed on the stimulable phosphor sheet 20.
The radiation data of the radiolabeled substance recorded in the above and the fluorescence data of the fluorescent dye etc. recorded in the many absorptive regions 4 of the biochemical analysis unit 1 can be read, and the laser beam 34 having a wavelength of 640 nm is used. The first laser excitation light source 31 which emits light and the laser light 3 having a wavelength of 532 nm.
Second laser excitation light source 32 which emits laser beam 4 and third laser excitation light source 33 which emits laser light 34 having a wavelength of 473 nm.
It has and.

In the present embodiment, the first laser excitation light source 31 is composed of a semiconductor laser light source, and the second laser excitation light source 32 and the third laser excitation light source 33.
Is composed of a second harmonic generation element.

The laser light 34 emitted from the first laser excitation light source 31 is collimated by the collimator lens 35 and then reflected by the mirror 46. In the optical path of the laser light 34 emitted from the first laser excitation light source 31 and reflected by the mirror 46, the first dichroic mirror 37 and 532 nm that transmit the laser light 4 of 640 nm and reflect the light of wavelength 532 nm. A second dichroic mirror 48 that transmits light of the above wavelength and reflects light of the wavelength of 473 nm is provided, and the laser light 34 generated by the first laser excitation light source 31 is provided.
Passes through the first dichroic mirror 37 and the second dichroic mirror 48 and enters the mirror 39.

On the other hand, the laser light 34 emitted from the second laser excitation light source 32 is made into parallel light by the collimator lens 40 and then reflected by the first dichroic mirror 37, and its direction is 90 degrees. After being changed, the light passes through the second dichroic mirror 48 and enters the mirror 39.

The laser light 34 emitted from the third laser excitation light source 33 is collimated by the collimator lens 41 into parallel light, which is reflected by the second dichroic mirror 48, and its direction is 90 degrees. After being changed, it is incident on the mirror 39.

The laser beam 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
A perforated mirror 44 formed by a concave mirror having a hole 43 formed in the center is arranged in the optical path of No. 4, and the laser light 34 reflected by the mirror 42 passes through the hole 43 of the perforated mirror 44. It passes through and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

The optical head 45 is provided with a mirror 46 and an aspherical lens 47. The laser light 34 incident on the optical head 45 is reflected by the mirror 46, and the glass plate of the stage 50 is reflected by the aspherical lens 47. It is incident on the stimulable phosphor sheet 20 placed on 51 or the biochemical analysis unit 1.

A laser beam 34 is applied to the stimulable phosphor sheet 20.
Is incident, the stimulable phosphor contained in the stimulable phosphor layer region 24 formed on the support 21 of the stimulable phosphor sheet 20 is excited, and stimulable light 55 is emitted. When the laser light 34 enters the biochemical analysis unit 1,
A fluorescent substance such as a fluorescent dye contained in the adsorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 is excited to emit fluorescence 55.

Photostimulated light 55 emitted from the stimulable phosphor layer region 24 of the stimulable phosphor sheet 20 or fluorescence 55 emitted from the absorptive region 4 of the biochemical analysis unit 1.
Is condensed on a mirror 46 by an aspherical lens 47 provided in the optical head 45, is reflected by the mirror 46 on the same side as the optical path of the laser light 34, becomes parallel light, and is incident on a concave mirror 48. To do.

The photostimulable light 55 or fluorescent light 55 which has entered the concave mirror 48 is reflected by the concave mirror 48,
The light enters the perforated mirror 44.

The photostimulable light 55 or fluorescence 55 that has entered the perforated mirror 44 is reflected downward by the perforated mirror 44 formed by a concave mirror and enters the filter unit 58, as shown in FIG. Then, the light of a predetermined wavelength is cut off, enters the photomultiplier 60, and is photoelectrically detected.

As shown in FIG. 8, the filter unit 58 includes four filter members 61a, 61b, 61c,
61d, the filter unit 58 is configured to be movable in the left-right direction in FIG. 8 by a motor (not shown).

FIG. 9 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 9, the filter member 61
a includes a filter 62a, and the filter 62a uses the first laser excitation light source 31 to perform the biochemical analysis unit 1
Is a filter member used when exciting the fluorescent substance contained in a large number of the absorptive regions 4 formed on the substrate 2 and reading the fluorescence 55 emitted from the absorptive regions 4.
It has a property of cutting light having a wavelength of 640 nm and transmitting light having a wavelength longer than 640 nm.

FIG. 10 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 10, the filter member 6
1b includes a filter 62b, and the filter 62b includes a second
The laser excitation light source 32 is used to excite the fluorescent substance contained in a large number of the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and the fluorescence 55 emitted from the absorptive regions 4 is excited. It is a filter member used when reading, and has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 11 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 11, the filter member 6
1c includes a filter 62c, and the filter 62c includes a third filter 62c.
The laser excitation light source 33 is used to excite the fluorescent substance contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and the fluorescence 55 emitted from the absorptive regions 4 is excited. It is a filter member used when reading, and has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 12 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 12, the filter member 6
1d includes a filter 62d, and the filter 62d includes a first
Of the stimulable phosphor sheet 2 using the laser excitation light source 31 of
0 is a filter used to excite a large number of photostimulable phosphor layer regions 24 formed on the support 21 and read out photostimulable light 55 emitted from the photostimulable phosphor layer regions 24. , Photostimulable light 55 emitted from the photostimulable phosphor layer region 24
Has a property of transmitting only the light in the wavelength range of, and cutting the light of the wavelength of 640 nm.

Therefore, the filter members 61a, 61b, 61c, 61d, depending on the laser excitation light source to be used.
Is selectively placed in front of the photomultiplier 60, the photomultiplier 60 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processor 64.

Although not shown in FIG. 7, the optical head 45 is configured to be movable in the main scanning direction indicated by arrow X and the sub scanning direction indicated by arrow Y in FIG. 7 by the scanning mechanism. And all stimulable phosphor layer regions 2 formed on the support 21 of the stimulable phosphor sheet 20.
4 or all absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are configured to be scanned by the laser light 34.

FIG. 13 is a schematic plan view of the scanning mechanism of the optical head.

In FIG. 13, for simplification, the optical system except the optical head 45 and the optical paths of the laser light 34 and the fluorescent light 55 or the stimulated light 55 are omitted.

As shown in FIG. 13, the optical head 45
The scanning mechanism that scans the substrate 70 includes a substrate 70, and a sub-scanning pulse motor 71 and a pair of rails 72 and 62 are provided on the substrate 70.
13 are fixed, and on the substrate 70, a substrate 7 that is movable in the sub-scanning direction indicated by an arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 73, and a threaded rod 74 rotated by the sub-scanning pulse motor 71 is formed in this hole. Engaged.

A main scanning stepping motor 75 is provided on the movable substrate 73, and the main scanning stepping motor 75 attaches the endless belt 76 to the adsorbents formed on the substrate 2 of the biochemical analysis unit 1 adjacent to each other. It is configured such that it can be driven intermittently at a pitch equal to the distance between the regions 4, that is, the adjacent stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20. The optical head 45 is fixed to the endless belt 76, and when the endless belt 76 is driven by the main scanning stepping motor 75, the optical head 45 is moved in the main scanning direction indicated by an arrow X in FIG. Has been done.

In FIG. 13, 67 is the optical head 45.
Is a linear encoder for detecting the position in the main scanning direction, and 78 is a slit of the linear encoder 77.

Therefore, the main scanning stepping motor 7
5, the endless belt 76 is intermittently driven in the main scanning direction, and when scanning of one line is completed, the substrate 73 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 71. The optical head 45 is
In FIG. 13, all the stimulable fluorescent light formed on the support 21 of the stimulable phosphor sheet 20 by the laser light 34 is moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y. The body layer region 24 or all absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned.

FIG. 14 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner shown in FIG.

As shown in FIG. 14, the scanner control system includes a control unit 80 for controlling the operation of the entire scanner, a data processing device 64, and a memory 55, and the scanner input system includes: A keyboard 81 that is operated by the user and can input various instruction signals
Is equipped with.

As shown in FIG. 14, the scanner drive system intermittently moves the optical head 45 in the main scanning direction and the main scanning stepping motor 75, and intermittently moves the optical head 45 in the sub scanning direction. Sub-scanning pulse motor 7
1 and 4 filter members 61a, 61b, 61c, 6
A filter unit motor 82 for moving the filter unit 58 having 1d is provided.

The control unit 80 includes a first laser pumping light source 31, a second laser pumping light source 32 or a third laser pumping light source 32.
In addition to selectively outputting the drive signal to the laser excitation light source 33, the drive signal can be output to the filter unit motor 82.

Further, as shown in FIG. 14, the detection system of the scanner is provided with a photomultiplier 60 and a linear encoder 77 for detecting the position of the optical head 45 in the main scanning direction.

In the present embodiment, the control unit 80 controls the first laser pumping light source 31, the second laser pumping light source 32, or the third laser pumping light source 32 according to the position detection signal of the optical head 45 input from the linear encoder 77. The laser excitation light source 33 is configured to be on / off controllable.

The scanner configured as described above accumulates with the radioactive labeling substance contained in the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 in the following manner. The stimulable phosphor contained in the large number of stimulable phosphor layer regions 24 of the phosphor sheet 20 is exposed and recorded in the large number of stimulable phosphor layer regions 24 of the stimulable phosphor sheet 20. Radiation data of the radiolabeled substance is read to generate biochemical analysis data.

First, the stimulable phosphor sheet 20 is placed on the glass plate 51 of the stage 50.

Then, the keyboard 8
An instruction signal for scanning a large number of stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20 with the laser light 34 is input to the first column.

The instruction signal input to the keyboard 81 is
Input to the control unit 80, the control unit 80 outputs a drive signal to the filter unit motor 82 in accordance with the instruction signal, and the filter unit 5
Photostimulable light 55 emitted from the stimulable phosphor by moving 8
The filter member 61d provided with the filter 62d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is located in the optical path of the stimulated light 55.

Further, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to move the optical head 45 in the main scanning direction, and based on the position detection signal of the optical head 45 inputted from the linear encoder, Of the large number of stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20, the first stimulable phosphor layer region 24 is located at a position where the laser beam 34 can be irradiated.
When it is confirmed that the optical head 45 has reached, a stop signal is output to the main scanning stepping motor 75, and
The drive signal is output to the first laser excitation light source 31 to
The laser excitation light source 31 is activated to emit laser light 34 having a wavelength of 640 nm.

The laser light 34 emitted from the first laser excitation light source 31 is made into parallel light by the collimator lens 35, and then enters the mirror 46 and is reflected.

Laser light 3 reflected by mirror 46
The light beam 4 passes through the first dichroic mirror 37 and the second dichroic mirror 48 and enters the mirror 39.

The laser light 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
4 passes through the hole 43 of the perforated mirror 44 and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

Laser light 34 incident on the optical head 45
Is reflected by the mirror 46, and by the aspherical lens 47, the first stimulable phosphor layer formed on the support 21 of the stimulable phosphor sheet 20 placed on the glass plate 51 of the stage 50. The light is focused on the area 24.

In this embodiment, each of the stimulable phosphor layer regions 24 of the stimulable phosphor sheet 20 is formed by:
In the through hole 22 formed in the nickel support 21,
Since the stimulable phosphor is formed by being embedded, the laser light 34 is scattered in the stimulable phosphor layer region 24 formed on the support 21 of the stimulable phosphor sheet 20 and is adjacent to the stimulable phosphor layer region 24. It is possible to effectively prevent the photostimulable phosphor contained in the adjacent photostimulable phosphor layer regions 24 from being excited by being incident on the combined photostimulable phosphor layer regions 24. become.

The laser light 34 emits the stimulable phosphor sheet 20.
First stimulable phosphor layer region 2 formed on the support 21 of
4 is incident on the support 21 of the stimulable phosphor sheet 20.
The stimulable phosphor contained in the first stimulable phosphor layer area 24 is excited by the laser light 34 to emit stimulable light from the first stimulable phosphor layer area 24. 55 is released.

The photostimulable light 55 emitted from the first photostimulable phosphor region 15 of the stimulable phosphor sheet 20 is collected by the aspherical lens 47 provided in the optical head 45,
The light is reflected by the mirror 46 to the same side as the optical path of the laser light 34, becomes parallel light, and enters the concave mirror 48.

The photostimulable light 55 incident on the concave mirror 48 is
The perforated mirror 44 is reflected by the concave mirror 48.
Incident on.

Photostimulated light 55 incident on the perforated mirror 44
Is reflected downward by the perforated mirror 44 formed by the concave mirror and enters the filter 62d of the filter unit 58, as shown in FIG.

The filter 62d transmits only the light in the wavelength range of the stimulable light 55 emitted from the stimulable phosphor, and emits 640n.
Since it has a property of cutting off light having a wavelength of m, light having a wavelength of 640 nm, which is excitation light, is cut, and light in the wavelength range of stimulable light 55 emitted from the stimulable phosphor layer region 24. Only the light passes through the filter 62d and is photoelectrically detected by the photomultiplier 60.

The analog signal photoelectrically detected by the photomultiplier 60 and generated is output to the A / D converter 63, converted into a digital signal, and output to the data processing device 64.

When a predetermined time, for example, several microseconds has elapsed after the first laser excitation light source 31 was turned on, the control unit 80 outputs a drive stop signal to the first laser excitation light source 31, The driving of the first laser excitation light source 31 is stopped, and a driving signal is output to the main scanning stepping motor 75 to move the optical head 45 to the adjacent luminescent elements formed on the support 21 of the stimulable phosphor sheet 20. It is moved by one pitch equal to the distance between the exhaustive phosphor layer regions 24.

On the basis of the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Are moved by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 24, and the first laser excitation light source 31
The stimulable phosphor sheet 2 emits the laser light 34 emitted from the
When it is confirmed that the second stimulable phosphor layer region 24 formed on the support 21 of 0 has moved to a position where irradiation is possible,
The control unit 80 includes the first laser excitation light source 3
The second stimulable phosphor layer region formed on the support 21 of the stimulable phosphor sheet 20 by the laser beam 34 by outputting a drive signal to the first laser excitation light source 31. The stimulable phosphor contained in No. 24 is excited.

Similarly, the laser beam 34 is irradiated for a predetermined time on the second stimulable phosphor layer region 24 formed on the support 21 of the stimulable phosphor sheet 20, and the second stimulable phosphor layer region 24 is irradiated.
The stimulable phosphor contained in the stimulable phosphor layer region 24 is excited, and the stimulable light 55 emitted from the second stimulable phosphor layer region 24 is emitted by the photomultiplier 60. When photoelectrically detected and analog data is generated, the control unit 80 outputs an off signal to the first laser pumping light source 31 and the first laser pumping light source 3
1 is turned off and the main scanning stepping motor 7
5, a drive signal is output to move the optical head 45 by one pitch equal to the distance between the adjacent photostimulable phosphor layer regions 24.

Thus, the first laser excitation light source 31 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 45, and based on the position detection signal of the optical head 45 inputted from the linear encoder 77, Optical head 45
Was moved by one line in the main scanning direction, and the scanning by the laser beam 34 of the stimulable phosphor layer region 24 of the first line formed on the support 21 of the stimulable phosphor sheet 20 was completed. If confirmed, the control unit 8
0 outputs a drive signal to the main scanning stepping motor 75 to return the optical head 45 to its original position, and outputs a drive signal to the sub-scanning pulse motor 71 to sub-scan the movable substrate 73. Only one line in the direction,
To move.

Based on the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is returned to its original position, and the movable substrate 73 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 80 determines that the stimulable phosphor layer of the first line formed on the support 21 of the stimulable phosphor sheet 20. The region 24 is sequentially irradiated with the laser light 34 emitted from the first laser excitation light source 31, and in the same manner as in the case where the region 24 is irradiated with the second line of the stimulable phosphor sheet 20 on the support 21. Of the stimulable phosphor layer region 2 on the second line by sequentially irradiating the luminescent phosphor layer region 24 with laser light 34 emitted from the first laser excitation light source 31.
The stimulable phosphor contained in No. 4 is excited, and the photomultiplier 60 sequentially photoelectrically detects the stimulable light 55 emitted from the stimulable phosphor layer region 24.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processing device 64.

Thus, all the stimulable phosphor layer regions 24 formed on the support 21 of the stimulable phosphor sheet 20.
Are scanned by the laser light 34 emitted from the first laser excitation light source 31, and all the stimulable phosphor layer regions 2 are scanned.
The stimulable phosphor contained in 4 is excited, and the emitted stimulable light 55 is photoelectrically detected by the photomultiplier 60, and the generated analog data is A / D.
When converted into digital data by the converter 63 and sent to the data processing device 64, a drive stop signal is sent from the control unit 80 to the first laser excitation light source 31.
And the driving of the first laser excitation light source 31 is stopped.

As described above, the stimulable phosphor sheet 2 is used.
The radiation data recorded in a large number of stimulable phosphor layer regions 24 of 0 are read to generate biochemical analysis data.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is read to generate digital data for biochemical analysis, first, The biochemical analysis unit 1 is set on the glass plate 51 of the stage 50 by the user.

Then, the keyboard 8 is set by the user.
A fluorescent substance identification signal that identifies the type of the fluorescent substance that is the labeling substance is input to 1, and an instruction signal that the fluorescence data should be read is input.

The fluorescent substance specifying signal and the instruction signal input to the keyboard 81 are input to the control unit 80, and when the control unit 80 receives the fluorescent substance specifying signal and the instruction signal, it is stored in a memory (not shown). The laser excitation light source to be used is determined according to the table shown in FIG.
It is determined which of b and 62c is to be located in the optical path of the fluorescent light 55.

For example, Rhodamine (registered trademark), which can be most efficiently excited by a laser having a wavelength of 532 nm, as a fluorescent substance for labeling a substance derived from a living body
Is used and is input to the keyboard 81, the control unit 80 selects the second laser excitation light source 32, selects the filter 62b, and outputs a drive signal to the filter unit motor 82. Then, the filter unit 58 is moved to cut off the light having the wavelength of 532 nm, and the filter member 61b having the property of transmitting the light having the wavelength longer than 532 nm is released from the biochemical analysis unit 1. It is located in the optical path of the fluorescent light 55 to be formed.

Further, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to move the optical head 45 in the main scanning direction, and based on the position detection signal of the optical head 45 inputted from the linear encoder, It is confirmed that the optical head 45 has reached the position where the laser beam 34 can be irradiated to the first absorptive region 4 among the many absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Then, a stop signal is output to the main scanning stepping motor 75 and the second laser excitation light source 32
To drive the second laser excitation light source 32 to emit the laser light 34 having a wavelength of 532 nm.

The laser light 34 emitted from the second laser excitation light source 32 is made into parallel light by the collimator lens 40, and then enters the first dichroic mirror 37 and is reflected.

The laser beam 34 reflected by the first dichroic mirror 37 passes through the second dichroic mirror 48 and enters the mirror 39.

The laser light 34 incident on the mirror 39 is reflected by the mirror 39 and further incident on the mirror 42 and reflected.

Laser light 3 reflected by mirror 42
4 passes through the hole 43 of the perforated mirror 44 and enters the concave mirror 48.

Laser light 34 incident on the concave mirror 48
Is reflected by the concave mirror 48, and the optical head 4
It is incident on 5.

Laser light 34 incident on the optical head 45
Is reflected by the mirror 46, and is focused by the aspherical lens 47 on the first absorptive region 4 of the biochemical analysis unit 1 placed on the glass plate 51 of the stage 50.

In the present embodiment, a large number of the absorptive regions 4 of the biochemical analysis unit 1 are filled with nylon 6 in the through holes 3 formed in the substrate made of aluminum, Since it is formed, the laser beam 34 is scattered in the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 and is incident on the absorptive regions 4 adjacent to each other, and the adjacent absorptive regions are adsorbed. It becomes possible to effectively prevent excitation of the fluorescent substance contained in the intrinsic region 4.

The laser beam 34 is emitted from the biochemical analysis unit 1
When it is incident on the first absorptive region 4 formed on the substrate 2, the fluorescent dye contained in the first absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 by the laser beam 34. A fluorescent substance such as, for example, rhodamine is excited to emit fluorescence.

The fluorescent light 55 emitted from the rhodamine is collected by the aspherical lens 47 provided in the optical head 45, reflected by the mirror 46 to the same side as the optical path of the laser light 34, and made into parallel light. , Enters the concave mirror 48.

The fluorescent light 55 that has entered the concave mirror 48 is reflected by the concave mirror 48 and enters the perforated mirror 44.

The fluorescence 55 that has entered the perforated mirror 44 is
The perforated mirror 44 formed by the concave mirror reflects the light downward as shown in FIG. 8 and makes it incident on the filter 62b of the filter unit 58.

Since the filter 62b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm, which is excitation light, is cut and emitted from rhodamine. Fluorescence 55
Only the light in the wavelength range of (4) passes through the filter 62b and is photoelectrically detected by the photomultiplier 60.

The analog signal photoelectrically detected by the photomultiplier 60 and generated is output to the A / D converter 63, converted into a digital signal, and output to the data processing device 64.

When a predetermined time, for example, several microseconds has elapsed after the second laser excitation light source 32 was turned on, the control unit 80 outputs a drive stop signal to the second laser excitation light source 32, The drive of the second laser excitation light source 32 is stopped, and a drive signal is output to the main scanning stepping motor 75 to cause the optical head 45 to be attached to the substrate 2 of the biochemical analysis unit 1 adjacent to each other. Move one pitch equal to the distance between regions 4.

Based on the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is moved by one pitch equal to the distance between the adjacent absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 to generate the laser light 34 emitted from the second laser excitation light source 32. When it is confirmed that the second adsorptive region 4 formed on the substrate 2 of the chemical analysis unit 1 has moved to a position where irradiation is possible, the control unit 80 causes the second
The second laser excitation light source 32 is turned on to turn on the second laser excitation light source 32, and the second light formed on the substrate 2 of the biochemical analysis unit 1 by the laser light 34.
The fluorescent substance contained in the absorptive region 4 of, for example, rhodamine is excited.

Similarly, the laser beam 34 is irradiated onto the second absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 for a predetermined time and is emitted from the second absorptive region 4. When the fluorescent light 55 is photoelectrically detected by the photomultiplier 60 and analog data is generated, the control unit 80 outputs an off signal to the second laser excitation light source 32, and the second laser The excitation light source 32 is turned off, and a drive signal is output to the main scanning stepping motor 75 to move the optical head 45 to a distance between adjacent absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Move one pitch equal to.

Thus, the first laser excitation light source 31 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 45, and based on the position detection signal of the optical head 45 inputted from the linear encoder 77, Optical head 45
Was moved by one line in the main scanning direction, and it was confirmed that all the absorptive regions 4 of the first line formed on the substrate 2 of the biochemical analysis unit 1 were scanned by the laser beam 34. Then, the control unit 80 outputs a drive signal to the main scanning stepping motor 75 to return the optical head 45 to the original position, and outputs a drive signal to the sub scanning pulse motor 71 to move the optical head 45. The substrate 73 is moved by one line in the sub-scanning direction.

On the basis of the position detection signal of the optical head 45 input from the linear encoder 77, the optical head 45
Is returned to its original position, and the movable substrate 73 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 80 sequentially moves to the absorptive region 4 of the first line formed on the substrate 2 of the biochemical analysis unit 1. , Is included in the absorptive region 4 of the second line formed on the substrate 2 of the biochemical analysis unit 1 in exactly the same manner as when the laser beam 34 emitted from the second laser excitation light source 32 is irradiated. The fluorescent light 55 emitted from the adsorptive region 4 of the second line by exciting the existing rhodamine,
The photomultiplier 60 sequentially detects photoelectrically.

The analog data photoelectrically detected by the photomultiplier 60 and generated are converted into digital data by the A / D converter 63 and sent to the data processing device 64.

In this way, all the absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 are scanned by the laser light 34 emitted from the second laser excitation light source 32, and the biochemical analysis unit 1 is scanned. Rhodamine contained in all the absorptive regions 4 formed on the substrate 2 is excited, and the emitted fluorescence 55 is converted into the photomultiplier 6
When the analog data photoelectrically detected and generated by 0 is converted into digital data by the A / D converter 63 and sent to the data processing device 64, the drive stop signal from the control unit 80 The second laser excitation light source 32 is output to the second laser excitation light source 32.
Is stopped.

As described above, the fluorescence data recorded in the many absorptive regions 4 of the biochemical analysis unit 1 is read, and the biochemical analysis data is generated.

FIG. 15 is a schematic front view of a data generation system for reading the chemiluminescence data recorded in the many absorptive regions 4 of the biochemical analysis unit 1 to generate the biochemical analysis data.

The data generation system shown in FIG.
The chemiluminescence data recorded in the many absorptive regions 4 of the biochemical analysis unit 1 can be read to generate biochemical analysis data, and at the same time, a large number of the absorptive regions 4 of the biochemical analysis unit 1 can be generated. It is configured so that the recorded fluorescence data of a fluorescent substance such as a fluorescent dye can be read to generate biochemical analysis data.

As shown in FIG. 15, the data generation system comprises a cooled CCD camera 91, a dark box 92 and a personal computer 93. The personal computer 93 includes a CRT 94 and a keyboard 95.

FIG. 16 is a schematic vertical sectional view of the cooled CCD camera 91.

As shown in FIG. 16, the cooled CCD camera 91 is disposed in front of the CCD 96, a heat transfer plate 97 made of metal such as aluminum, a Peltier element 98 for cooling the CCD 86, and the CCD 96. Shutter 99, and A / D converter 100 for converting analog data generated by CCD 96 into digital data,
A data buffer 101 for temporarily storing the data digitized by the A / D converter 100, and a cooling CC
And a camera control circuit 102 for controlling the operation of the D camera 91.

The opening formed between the dark box 82 and the dark box 82 is closed by a glass plate 105, and a radiation fin 106 for radiating the heat generated by the Peltier element 98 is provided around the cooling CCD camera 91. It is formed over almost the entire surface in the direction.

Inside the dark box 92 in front of the glass plate 105,
Camera lens 107 having lens focus adjustment function
Is installed.

FIG. 17 is a schematic vertical sectional view of the dark box 92.

As shown in FIG. 17, an LED light source 110 which emits excitation light is provided in the dark box 92, and the LED light source 110 is provided with a filter 111 which is detachably provided and an upper surface of the filter 111. The diffusion plate 113 is provided, and the excitation light is emitted through the diffusion plate 113 toward a biochemical analysis unit (not shown) mounted thereon. The chemical analysis unit is guaranteed to be evenly illuminated.
The filter 111 has a property of cutting light harmful to the excitation of the fluorescent substance other than the wavelength near the excitation light and transmitting only the light of the wavelength near the excitation light. On the front surface of the camera lens 107, a filter 112 that cuts off light having a wavelength near the excitation light is detachably provided.

FIG. 18 is a block diagram of the periphery of the personal computer 93 which constitutes the data generating system.

As shown in FIG. 17, the personal computer 93 comprises a CPU 120 for controlling the exposure of the cooled CCD camera 81, a data transfer means 121 for reading out the digital data generated by the cooled CCD camera 91 from the data buffer 101, and a digital converter. Based on the data storage means 122 for storing data, the data processing means 123 for performing data processing on the digital data stored in the data storage means 122, and the digital data stored in the data storage means 122, on the screen of the CRT 84. Data display means 124 for displaying visible data is provided.

The LED light source 110 has a light source control means 125.
The light source control unit 125 is configured to receive an instruction signal from the keyboard 95 via the CPU 120. The CPU 120 is configured to be able to output various signals to the camera control circuit 102 of the cooled CCD camera 91.

The data generation system shown in FIGS. 15 to 18 is derived from a living body selectively hybridized with a specific binding substance contained in many absorptive regions 4 of the biochemical analysis unit 1. The hapten, such as digoxigenin, which labels the substance of (1), reacts with the enzyme that labels the antibody to the hapten bound by the antigen-antibody reaction, and the chemiluminescence generated by the contact with the chemiluminescent substrate is passed through the camera lens 107. , CCD96 of cooled CCD camera 91
The chemiluminescence data recorded in the large number of absorptive regions 4 of the biochemical analysis unit 1 are read to generate biochemical analysis data and are formed on the substrate 2 of the biochemical analysis unit 1. Excited light is emitted from the LED light source 110 to the adsorbed regions 4 thus prepared to excite a fluorescent substance such as a fluorescent dye selectively contained in the adsorbed regions 4 of the biochemical analysis unit 1. , The fluorescence emitted from the fluorescent substance is passed through the camera lens 107 to cool the CCD.
The fluorescence data recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1 detected by the CCD 96 of the camera 91 are read, and biochemical analysis data can be generated.

When reading the chemiluminescence data, with the filter 112 removed and the LED light source 110 held in the off state, a large number of absorptive regions 4 of the biochemical analysis unit 1 are selected on the diffusion plate 113. The chemiluminescent substrate is contacted with the labeling enzyme that labels the antibody contained in the
The biochemical analysis unit 1 that emits chemiluminescence is placed.

Then, the camera lens 1 is set by the user.
07, the lens focus is adjusted, and the dark box 92
Is closed.

After that, when the user inputs an exposure start signal to the keyboard 95, the exposure start signal changes to the CPU 12
0, the camera control circuit 1 of the cooled CCD camera 91
02, and the camera control circuit 102 opens the shutter 99 to start the exposure of the CCD 96.

The chemiluminescence emitted from the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is transmitted through the camera lens 107 to the C of the cooled CCD camera 91.
It is incident on the photocathode of CD96 to form an image on the photocathode.

The CCD 96 thus receives the light of the image formed on the photocathode and stores it in the form of electric charges.

In this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum having the property of attenuating light, the chemiluminescence emitted from the labeling enzyme is biochemical. It can be reliably prevented from being scattered within the substrate 2 of the analysis unit 1 and being mixed with the chemiluminescence emitted from the labeling enzyme contained in the adsorbing regions 4 adjacent to each other.

When the predetermined exposure time has elapsed, the CPU 12
0 outputs an exposure completion signal to the camera control circuit 102 of the cooled CCD camera 91.

Upon receiving the exposure completion signal from the CPU 120, the camera control circuit 102 transfers the analog data accumulated by the CCD 96 in the form of electric charge to the A / D converter 100, digitizes it, and temporarily stores it in the data buffer 101. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 102, the CPU 120 causes the data transfer means 1 to operate.
21 outputs a data transfer signal to the cooled CCD camera 9
The digital data is read from the first data buffer 101 and stored in the data storage means 122.

When the user inputs a data processing signal to the keyboard 95, the CPU 120 causes the data processing means 123 to output the digital data stored in the data storage means 122, and the data processing means 123.
Then, according to the user's instruction, the data processing is executed and stored in the data storage means 122.

Next, when the user inputs a data display signal to the keyboard 95, the CPU 120 outputs the data display signal to the data display means 124, and the biochemical analysis is carried out based on the digital data stored in the data storage means 122. Data is displayed on the screen of the CRT 94.

In this way, the chemiluminescence data recorded in the many absorptive regions 4 of the biochemical analysis data 1 is read, and the biochemical analysis data is generated.

On the other hand, when the fluorescence data recorded in the many absorptive regions 4 of the biochemical analysis data 1 is read to generate the biochemical analysis data, first, the biochemical analysis unit 1 Are placed on the diffusion plate 103.

Next, the LED light source 11 is selected by the user.
0 is turned on, the lens focus is adjusted using the camera lens 107, and the dark box 92 is closed.

Then, when the user inputs an exposure start signal to the keyboard 95, the light source control means 125 turns on the LED light source 110, and the excitation light is emitted toward the biochemical analysis unit 1. At the same time, the exposure start signal is sent to the cooling CCD camera 9 via the CPU 120.
1 is input to the camera control circuit 102, the camera control circuit 102 opens the shutter 99, and the exposure of the CCD 96 is started.

The excitation light emitted from the LED light source 110 is filtered by the filter 111 to eliminate wavelength components other than those in the vicinity of the wavelength of the excitation light, and made into uniform light by the diffusion plate 113, and the biochemical analysis unit 1 Is irradiated.

As a result, the fluorescent substance selectively contained in the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is excited by excitation light to emit fluorescence. .

Fluorescence emitted from a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is cooled through the filter 112 and the camera lens 107.
The light enters the photoelectric surface of the CCD 96 of the D camera 91 and forms an image on the photoelectric surface.

Since the filter 112 cuts off the light of the wavelength of the excitation light, only the fluorescence emitted from the fluorescent substance selectively contained in the many absorptive regions 4 of the biochemical analysis unit 1 is cut off. Is received by the CCD 96.

The CCD 96 thus receives the light of the image formed on the photocathode and accumulates it in the form of electric charges.

Here, in the present embodiment, the substrate 2 of the biochemical analysis unit 1 is formed of aluminum having a property of attenuating light, so that fluorescence emitted from a fluorescent substance such as a fluorescent dye is emitted. , The adsorptive regions 4 adjacent to each other scattered in the substrate 2 of the biochemical analysis unit 1
It is possible to reliably prevent the fluorescent light emitted from the mixture from mixing.

[0307] When the predetermined exposure time has elapsed, the CPU 12
0 outputs an exposure completion signal to the camera control circuit 102 of the cooled CCD camera 91.

Upon receiving the exposure completion signal from the CPU 120, the camera control circuit 102 transfers the analog data accumulated by the CCD 96 in the form of electric charge to the A / D converter 100, digitizes it, and temporarily stores it in the data buffer 101. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 102, the CPU 120 controls the data transfer means 1
21 outputs a data transfer signal to the cooled CCD camera 9
The digital data is read from the first data buffer 101 and stored in the data storage means 122.

When the user inputs a data processing signal to the keyboard 95, the CPU 120 causes the data processing means 123 to output the digital data stored in the data storage means 122, and the data processing means 123.
Then, according to the user's instruction, the data processing is executed and stored in the data storage means 122.

Next, when the user inputs a data display signal to the keyboard 95, the CPU 120 outputs the data display signal to the data display means 124, and the biochemical analysis based on the digital data stored in the data storage means 122. Data is displayed on the screen of the CRT 94.

According to this embodiment, a large number of line-shaped electrodes 14a, 14b, 14c, which form the first electrode group 14,
...... Selects a predetermined linear electrode out of 14 m and applies a positive or negative voltage to it to generate a second electrode group 15
A large number of line-shaped electrodes 15a, 15b, 15
15n of c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, a living-body-derived solution contained in the hybridization reaction solution filled in the reaction container 13 is selected. The substance is attracted to a predetermined linear electrode to which a positive voltage is applied, and the substance is forcibly crossed across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Since it can be moved, biochemical analysis can be performed compared to the case where the substance of biological origin contained in the hybridization reaction solution is simply moved by convection or diffusion and hybridized with a specific binding substance. The transfer rate of the substance derived from the living body in the absorptive region 4 of the unit for use 1 can be greatly increased, and therefore the hybrid It is possible to greatly improve the reaction rate of the zeation reaction, and further to greatly increase the probability that a substance of biological origin encounters a specific binding substance contained in the deep part of the adsorptive region 4. Can
Therefore, as desired, it is possible to hybridize the substance of biological origin contained in the hybridization reaction solution with the specific binding substance immobilized on the large number of adsorptive regions 4 of the biochemical analysis unit 1. become.

Further, according to the present embodiment, the biochemical analysis unit 1 does not have to be provided with a pair of electrodes for each of the large number of absorptive regions 4 formed on the substrate 2, but is simply provided with the first electrode. A large number of linear electrodes 1 forming the electrode group 14
A large number of line-shaped electrodes 15a, 15b, 15c forming a second electrode group 15 by selecting a predetermined line-shaped electrode from among 4a, 14b, 14c, ... 14m and applying a positive or negative voltage. , 15d, ... out of 15n
By selecting a predetermined linear electrode and applying a negative or positive voltage, the bio-derived substance contained in the hybridization reaction solution filled in the reaction vessel 13 can be biochemically reacted as desired. Biochemical analysis unit 1 held by analysis unit holding member 12
Since it can be forcibly moved across a large number of adsorptive regions 4, it is possible to greatly improve the reaction rate of the hybridization reaction by using a reactor having a simple structure and a simple reaction operation. Furthermore, it is possible to significantly increase the probability that a substance derived from a living body encounters a specific binding substance contained in a deep portion of the adsorptive region 4, and therefore, as desired, for biochemical analysis. It becomes possible to hybridize the substance of biological origin contained in the hybridization reaction solution with the specific binding substance immobilized on the numerous absorptive regions 4 of the unit 1.

Further, the hybridization reaction solution is circulated in the reaction container 13 via the solution circulation pipe using a pump or the like, and a large number of adsorptions formed on the substrate 2 of the biochemical analysis unit 1 are adsorbed. In the case where the reaction container 13 and the solution circulation pipe are to be flowed across the property region 4, it is necessary to fill the inside of the reaction container 13 and the solution circulation conduit with the hybridization reaction solution, and a hybridization reaction solution having a high concentration of a substance derived from a living body is used. Difficult to do,
According to the present embodiment, simply a large number of linear electrodes 14a, 14b, 14c, ...
A predetermined number of line-shaped electrodes 15a, 15b, 15c, 15 forming a second electrode group 15 by selecting a predetermined line-shaped electrode of 4m and applying a positive or negative voltage.
d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the substance derived from the living body contained in the hybridization reaction solution filled in the reaction vessel 13 is removed. , Is attracted to a predetermined linear electrode to which a positive voltage is applied, and is forcibly moved across a large number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Therefore, the reaction vessel 13 having a small volume was filled with the hybridization reaction solution having a high concentration of the substance derived from the living body and fixed to the large number of absorptive regions 4 of the biochemical analysis unit 1. Specific binding substances can be hybridized with substances of biological origin contained in the hybridization reaction solution, and The reaction rate of lida homogenization reaction it is possible to increase significantly.

Further, according to the present embodiment, simply, the large number of line-shaped electrodes 14a, 14 constituting the first electrode group 14 are formed.
b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a, 1a constituting the second electrode group 15.
Among the 5b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the uniqueness contained in the absorptive region 4 of the biochemical analysis unit 1 is selected. A positive voltage is applied to a substance derived from a living body, which is not hybridized to a chemically bound substance but is adsorbed to a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Since it can be attracted to the line-shaped electrode that is present and separated from the adsorptive region 4 of the biochemical analysis unit 1, the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 It is possible to prevent deterioration of the quantification due to the fact that the non-hybridized substance derived from the living body is adsorbed to the absorptive region 4 of the biochemical analysis unit 1 and to obtain the bioassay with excellent quantification. Chemistry It is possible to generate 析用 data.

Further, according to this embodiment, a large number of line-shaped electrodes 14a, 14b, which constitute the first electrode group 14,
14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto, and a large number of line-shaped electrodes 15a, 15 constituting the second electrode group 15 are selected.
b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the antibody contained in the antibody solution filled in the reaction container 13 is By attracting to a predetermined linear electrode to which a positive voltage is applied, and forcibly moving it across a number of absorptive regions 4 of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Therefore, as compared with the case where the antibody contained in the antibody solution is simply moved by convection or diffusion to bind to the antigen, the antibody in the adsorptive region 4 of the biochemical analysis unit 1 It is possible to greatly increase the migration rate, and thus, it is possible to significantly improve the reaction rate of the antigen-antibody reaction. Furthermore, the hapte contained in the antibody solution can be increased. It is possible to significantly increase the probability that the antibody against will selectively meet with the specific binding substance contained in the deep portion of the adsorptive region 4 and the hapten labeling the hybridized substance of biological origin. Therefore, as desired, the antibody against the hapten contained in the antibody solution and the adsorptive region 4 of the biochemical analysis unit 1 can be obtained.
It becomes possible to selectively bind the specific binding substance contained in (1) to the hapten labeling the hybridized substance derived from the living body by the antigen-antibody reaction.

Further, according to this embodiment, the biochemical analysis unit 1 does not have to be provided with a pair of electrodes for each of the large number of absorptive regions 4 formed on the substrate 2, but is simply provided with a first electrode. A large number of linear electrodes 1 forming the electrode group 14
A large number of line-shaped electrodes 15a, 15b, 15c forming a second electrode group 15 by selecting a predetermined line-shaped electrode from among 4a, 14b, 14c, ... 14m and applying a positive or negative voltage. , 15d, ... out of 15n
By selecting a predetermined linear electrode and applying a negative or positive voltage, the antibody contained in the antibody solution filled in the reaction vessel 13 can be retained as desired in a unit holding member for biochemical analysis. Across a number of absorptive regions 4 of the biochemical analysis unit 1 held by 12,
Since it can be forcibly moved, it is possible to greatly improve the reaction rate of the antigen-antibody reaction by using a reactor with a simple structure and a simple reaction operation. The antibody against the existing hapten significantly increases the probability that the specific binding substance contained in the deep part of the adsorptive region 4 selectively and selectively encounters the hapten labeling the hybridized substance of biological origin. Therefore, as desired, the antibody to the hapten contained in the antibody solution and the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 can be selectively hybridized. The hapten labeling the substance derived from the living body can be bound by an antigen-antibody reaction.

Further, the antibody solution was circulated in the reaction vessel 13 through the solution circulation pipe using a pump or the like, and a large number of absorptive regions formed on the substrate 2 of the biochemical analysis unit 1 were circulated. In the case of flowing across 4 the reaction container 13 and the solution circulation conduit must be filled with the antibody solution, and it is difficult to use the antibody solution having a high antibody concentration. According to the above, simply, a large number of line-shaped electrodes 14a forming the first electrode group 14 are provided.
14b, 14c, ... 14m, a predetermined linear electrode is selected, and a positive or negative voltage is applied,
A large number of linear electrodes 15 forming the second electrode group 15
a, 15b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the antibodies contained in the antibody solution filled in the reaction container 13 Is attracted to a predetermined linear electrode to which a positive voltage is applied, and the biochemical analysis unit 1 is held by the biochemical analysis unit holding member 12.
It is possible to forcibly move it across a large number of absorptive regions 4 of the biochemical analysis unit 1 by filling the reaction vessel 13 having a small volume with an antibody solution having a high antibody concentration. A hapten such as digoxigenin that selectively labels a substance of biological origin hybridized with a specific binding substance contained in a large number of adsorptive regions 4 formed on the substrate 2, and a chemiluminescent substrate. An antibody against a hapten labeled with an enzyme that causes chemiluminescence when contacted can be bound by an antigen-antibody reaction, and the reaction rate of the antigen-antibody reaction can be significantly increased.

Further, according to the present embodiment, simply, the large number of line-shaped electrodes 14a, 14 constituting the first electrode group 14 are formed.
b, 14c, ... 14m, a predetermined line-shaped electrode is selected, and a positive or negative voltage is applied thereto to form a large number of line-shaped electrodes 15a, 1a constituting the second electrode group 15.
Among the 5b, 15c, 15d, ... 15n, by selecting a predetermined linear electrode and applying a negative or positive voltage, the uniqueness contained in the absorptive region 4 of the biochemical analysis unit 1 is selected. To a hapten such as digoxigenin that selectively labels the hybridized substance derived from the living body by the antigen-antibody reaction even though it is not bound to the specific binding substance by the biochemical analysis unit 1 An antibody adsorbed on a large number of absorptive regions 4 formed on the substrate 2 is attracted to a linear electrode to which a positive voltage is applied, and is separated from the absorptive regions 4 of the biochemical analysis unit 1. Therefore, the specific binding substance contained in the adsorptive region 4 of the biochemical analysis unit 1 is selectively labeled with the substance derived from the living body to which it is hybridized. By preventing antigen-antibody reaction from binding to a hapten such as genin, an antibody that is not bound is adsorbed to the absorptive region 4 of the biochemical analysis unit 1 to prevent a decrease in the quantification, thereby improving the quantification. It becomes possible to generate excellent biochemical analysis data.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above-mentioned embodiment, the substance derived from the living body labeled with the radioactive labeling substance and the substance derived from the living body labeled with the fluorescent substance are adsorbed on the large number of absorptive regions 4 of the biochemical analysis unit 1. The specific binding substance is selectively hybridized with a large number of absorptive regions 4 of the biochemical analysis unit 1.
The specific binding substance adsorbed on the enzyme is selectively hybridized with a substance of biological origin labeled with a hapten such as digoxigenin, and further labeled with an enzyme that causes chemiluminescence by contacting with a chemiluminescent substrate. The antibody against the hapten thus obtained is configured to bind to a hapten such as digoxigenin that labels a substance of biological origin by an antigen-antibody reaction,
The present invention is not limited to such hybridization reaction and antigen-antibody reaction,
It can be widely applied to all antigen-antibody reactions, and further to all receptor-ligand association reactions.

In the above embodiment, the specific binding substance immobilized on the adsorptive region 4 of the biochemical analysis unit 1 is hybridized with a substance of biological origin labeled with a hapten such as digoxigenin, and , An antibody against a hapten such as digoxigenin that is labeled with an enzyme that causes chemiluminescence by contacting with a chemiluminescent substrate is bound to a hapten such as digoxigenin that labels a substance of biological origin by an antigen-antibody reaction. , Is configured to selectively record chemiluminescence data in a large number of absorptive regions 4 of the biochemical analysis unit 1, but is labeled with a labeling substance that causes chemiluminescence by contact with a chemiluminescence substrate. The bio-derived substance is immobilized on a large number of absorptive regions 4 of the biochemical analysis unit 1. The specific binding substances are, selectively, by hybridizing, in a number of the absorptive regions 4 of the biochemical analysis unit 1, may be recorded chemiluminescence data.

Furthermore, in the above-mentioned embodiment, a specific binding substance such as cDNA immobilized on a large number of adsorptive regions 4 of the biochemical analysis unit 1 is labeled with a substance derived from a living body labeled with a fluorescent substance. It is configured to selectively hybridize and record fluorescence data in a large number of absorptive regions 4 of the biochemical analysis unit 1.
A specific binding substance such as cDNA immobilized on a large number of adsorptive regions 4 of the biochemical analysis unit 1 is selectively hybridized with a substance of biological origin labeled with a hapten such as digoxigenin, and , A unit for biochemical analysis by binding an antibody against a hapten labeled with an enzyme that produces a fluorescent substance by contacting with a fluorescent substrate to a hapten labeling a substance of biological origin by an antigen-antibody reaction It is also possible to record fluorescence data in a large number of absorptive regions 4 in one.

Further, in the above-mentioned embodiment, the reaction solution contains a substance of biological origin labeled with a radioactive labeling substance and a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye. It is not always necessary to include a biological substance labeled with a radioactive labeling substance and a biological substance labeled with a fluorescent substance such as a fluorescent dye, and the radioactive labeling substance, a fluorescent substance such as a fluorescent dye and a chemical substance It suffices to include a substance derived from a living body that is labeled with at least one type of labeling substance that produces chemiluminescence when brought into contact with a luminescent substrate.

Further, in the above embodiment, first,
A large number of linear electrodes 14 forming the first electrode group 14
14m of a, 14b, 14c, ... 14m, a negative voltage is supplied to the odd-numbered linear electrodes 14a, 14c ,. 15b, 15c, 15d, ... 1
Of the 5n, odd-numbered linear electrodes 15a, 15c,
Is supplied with a positive voltage, and then a large number of line-shaped electrodes 14a, 14b, 1 forming the first electrode group 14 are supplied.
4c, ... 14m of even-numbered line-shaped electrodes 14m
A large number of line-shaped electrodes 1 that form a second electrode group 15 while supplying a positive voltage to b, 14d, ...
Of the 5a, 15b, 15c, 15d, ... 15n, a negative voltage is supplied to the odd-numbered linear electrodes 15a, 15c ,. Electrodes 14a, 14b, 14c, ... 1
Of the 4m, even-numbered linear electrodes 14b, 14d,
... is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15 forming the second electrode group 15 are provided.
15n of b, 15c, 15d, ... 15n, a positive voltage is supplied to the even-numbered linear electrodes 15b, 15d ,. , 14b, 14c, ... Of 14m,
A large number of line-shaped electrodes 15a, 15b, 15c, 15 forming the second electrode group 15 are provided while supplying a positive voltage to the odd-numbered line-shaped electrodes 14a, 14c, ....
d ... 15n of the line-shaped electrodes 15n
A large number of line-shaped electrodes 1 that supply a negative voltage to b, 15d, ... And further constitute the first electrode group 14
Of the line electrodes 4a, 14b, 14c, ... 14m, a negative voltage is supplied to the odd-numbered line electrodes 14a, 14c ,. 15b, 15c, 15d, ...
Of the 15n, odd-numbered linear electrodes 15a, 15
The steps of supplying a positive voltage to c, ... Are repeated to carry out the hybridization reaction and the antigen-antibody reaction. However, it is not always necessary to repeat such steps, and at each step,
A large number of linear electrodes 14 forming the first electrode group 14
14m of a, 14b, 14c, ... 14m are selected, and a positive or negative voltage is applied, and a large number of linear electrodes 1 constituting the second electrode group 15 are selected.
Of the 5a, 15b, 15c, 15d, ... 15n, which line-shaped electrode is selected to apply the negative or positive voltage can be arbitrarily determined.
According to a certain rule, a large number of line-shaped electrodes 14a, 14b, 14c, ...
Of the 4m, odd-numbered linear electrodes 14a, 14c,
... is supplied with a negative voltage, and a large number of line-shaped electrodes 15a, 15 forming the second electrode group 15 are provided.
It is not always necessary to repeat the step of supplying a positive voltage to the odd-numbered linear electrodes 15a, 15c, ... Of b, 15c, 15d ,.

Further, in the above-described embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of aluminum and has conductivity, the biochemical analysis unit holding the biochemical analysis unit 1 is held. The member 12 includes the line-shaped electrodes 14 a, 14 that form the first electrode group 14.
b, 14c, 14d, ... 14m and the line-shaped electrodes 15a forming the second electrode group 15,
15b, 15c, 15d, ... 15n is set to an intermediate potential between the potentials to be given, for example, 0, but the substrate 2 of the biochemical analysis unit 1 is made of a non-conductive material such as silicon nitride. When it is formed, it is not necessary to set the biochemical analysis unit holding member 12 to a specific potential.

Further, in the above embodiment, the biochemical analysis unit 1 includes the biochemical analysis unit holding member 12.
Is configured to be held substantially horizontally in the reaction container 13, and as a result, the first electrode group 14 is located above the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. Is provided in the second electrode group 15
Is provided below the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. The biochemical analysis unit 1 is held substantially horizontally by the biochemical analysis unit holding member 12. , It is not always necessary to hold it in the reaction vessel 13, and therefore the first electrode group 14 is provided on one side of the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12. , The second electrode group 15, the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12
It suffices if it is provided on the other side of the first electrode group 14
Is provided above the biochemical analysis unit 1 held by the biochemical analysis unit holding member 12, and the second electrode group 15 is held by the biochemical analysis unit holding member 12 It is not always necessary to provide below 1.

Further, in the above embodiment, 192
The substantially circular adsorptive regions 4 having a size of about 0.01 square millimeters of 00 are formed in the biochemical analysis unit 1 in a matrix according to a regular pattern with a density of about 5000 pieces / square centimeter. However, it is not always necessary to form the absorptive region 4 in a substantially circular shape, and the absorptive region 4 can be formed in an arbitrary shape, for example, a rectangular shape.

In the above embodiment, 1920
An approximately circular adsorptive region 4 having a size of about 0.01 square millimeters of 0 is formed on the biochemical analysis unit 1 in a matrix according to a regular pattern with a density of about 5000 pieces / square centimeter. However, the number and size of the absorptive regions 4 may vary depending on the purpose.
The adsorbent region 4 is arbitrarily selected and preferably has a size of 10 or more and less than 5 mm 2.
Are formed in the biochemical analysis unit 1 at a density of 10 pieces / square centimeter or more.

Further, in the above embodiment, 192
The substantially circular adsorptive regions 4 having a size of about 0.01 square millimeters of 00 are formed in the biochemical analysis unit 1 in a matrix according to a regular pattern with a density of about 5000 pieces / square centimeter. However, it is not always necessary to form the absorptive region 4 of the biochemical analysis unit 1 in the biochemical analysis unit 1 according to a regular pattern.

Further, in the above-mentioned embodiment, the biochemical analysis unit 1 has nylon 6 filled inside the large number of through holes 3 formed in the aluminum substrate 2,
Although it has a large number of absorptive regions 4 formed, it is not always necessary that the absorptive regions 4 of the biochemical analysis unit 1 are formed of nylon 6, and instead of nylon 6, activated carbon or the like is used. Porous carbon materials or nylons such as nylon 6,6, nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginates such as alginic acid, calcium alginate, alginic acid / polylysine polyion complex Polyolefins such as polyethylene and polypropylene; Polyvinyl chloride; Polyvinylidene chloride; Polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and adsorption of biochemical analysis unit 1 by a copolymer or complex thereof. Sex area 4 Can be formed, furthermore,
Metals such as platinum, gold, iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate and inorganic porous materials such as composites thereof or more The absorptive region 4 of the biochemical analysis unit 1 may be formed by the bundle of fibers.

Further, in the above embodiment, the biochemical analysis unit 1 is provided with the substrate 2 made of aluminum, but it is not always necessary to form the substrate 2 of the biochemical analysis unit 1 with aluminum. Alternatively, the substrate 2 can be formed of another material. The substrate 2 of the biochemical analysis unit 1 is preferably formed of a material having a property of attenuating light and radiation, but the material is not particularly limited and may be an inorganic compound material or an organic compound material. In any case, the biochemical analysis unit 1 substrate 2 can be formed, and a metal material, a ceramic material or a plastic material is particularly preferably used. Examples of the inorganic compound material that can be preferably used to form the substrate 2 of the biochemical analysis unit 1 and have the property of attenuating light and radiation include gold, silver, copper, zinc, aluminum, titanium, Metals such as tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; aluminum oxide, Examples thereof include metal oxides such as magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, as the organic compound material which can be preferably used for forming the substrate 2 of the biochemical analysis unit 1 and has a property of attenuating light and radiation, a polymer compound is preferably used, and a preferable polymer compound is used. For example, polyolefins such as polyethylene and polypropylene; polymethylmethacrylate, butylacrylate /
Acrylic resins such as methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylon 6 Polyamide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose Polysaccharides such as cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; ; Chitosan; lacquer; gelatin, collagen, copolymers of polyamides and these high molecular compounds, such as keratin, and the like.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Further, in the above-mentioned embodiment, the many absorptive regions 4 of the biochemical analysis unit 1 are formed by filling the many through holes 3 formed in the substrate 2 with nylon 6. However, it is also possible to form a large number of absorptive regions 4 by press-fitting an absorptive film formed of an absorptive material such as nylon 6 into a large number of through holes formed in the substrate.

Further, in the above-mentioned embodiment, in the many absorptive regions 4 of the biochemical analysis unit 1, the many through holes 3 formed in the substrate 2 are filled with nylon 6,
Although formed, a substrate with a large number of through holes is adhered to at least one side of the absorptive substrate formed of an absorptive material such as a membrane filter, and a large number of absorptive substrates inside the through holes are used. It is also possible to form the absorptive region 4 of.

Further, in the above embodiment, the large number of absorptive regions 4 of the biochemical analysis unit 1 are formed by filling the large number of through holes 3 formed in the substrate 2 with nylon 6. However, a solution containing a specific binding substance is dropped onto a adsorptive substrate formed of an adsorptive material such as a membrane filter according to a regular pattern, for example, in a matrix of 120 columns × 160 rows to form a specific binding substance. The absorptive regions 4 containing the binding substance may be formed separately from each other.

[0336]

INDUSTRIAL APPLICABILITY According to the present invention, a ligand or a receptor immobilized on a biochemical analysis unit can be efficiently caused to undergo an association reaction with the receptor or the ligand, and further, the reproducibility and the quantification are excellent. It becomes possible to provide a receptor-ligand association reaction method and a reactor used for the same, which makes it possible to generate biochemical analysis data.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit used in a receptor-ligand association reaction method according to a preferred embodiment of the present invention.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic vertical cross-sectional view of a reactor used in the method of receptor-ligand association reaction according to a preferred embodiment of the present invention.

FIG. 4 is a schematic plan view of a first electrode group and a second electrode group.

FIG. 5 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 6 is a schematic perspective view of a stimulable phosphor sheet. A large number of stimulable phosphor sheets are formed on the stimulable phosphor sheet by a radioactive labeling substance contained in a large number of absorptive regions formed in a biochemical analysis unit. FIG. 3 is a schematic cross-sectional view showing a method of exposing the photostimulable phosphor layer region of FIG.

FIG. 7 is a diagram showing radiation data of radiolabeled substances recorded in a large number of stimulable phosphor layer regions formed on a support of a stimulable phosphor sheet and formed on a substrate of a unit for biochemical analysis. It is a schematic perspective view of the scanner which reads the fluorescence data recorded on the many absorptive area | regions produced, and produces | generates the data for biochemical analysis.

8 is a schematic perspective view showing details near the photomultiplier of the scanner shown in FIG. 7. FIG.

9 is a schematic cross-sectional view taken along the line AA of FIG.

10 is a schematic cross-sectional view taken along the line BB of FIG.

11 is a schematic cross-sectional view taken along the line CC of FIG.

12 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 13 is a schematic plan view of a scanning mechanism of an optical head.

14 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner shown in FIG.

FIG. 15 is a schematic front view of a data generation system that reads chemiluminescence data recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1 to generate biochemical analysis data.

FIG. 16 is a schematic vertical sectional view of a cooled CCD camera.

FIG. 17 is a schematic vertical sectional view of a dark box.

FIG. 18 is a block diagram of the periphery of a personal computer included in the data generation system.

[Explanation of symbols]

1 biochemical analysis unit 2 substrate 3 through hole 4 adsorptive region 5 injector 6 CCD camera 10 lid member 11 casing 12 biochemical analysis unit holding member 13 reaction vessel 14 first electrode group 14a, 14b, 14c, 14d, ...... 14m Line-shaped electrode 15 Second electrode group 15a, 15b, 15c, 15d, ...... 15n Line-shaped electrode 20 Storage phosphor sheet 21 Support 22 Through hole 24 Photostimulable phosphor layer region 31 First Laser excitation light source 32 Second laser excitation light source 33 Third laser excitation light source 34 Laser light 35 Collimator lens 36 Mirror 37 First dichroic mirror 38 Second dichroic mirror 39 Mirror 40 Collimator lens 41 Collimator lens 42 Mirror 43 Perforated Mirror hole 44 Perforated mirror 45 Optical head 46 Mirror 4 7 Aspherical lens 48 Concave mirror 50 Stage 51 Glass plate 55 Fluorescent or stimulated light 58 Filter unit 60 Photomultipliers 61a, 61b, 61c, 61d Filter members 62a, 62b, 62c, 62d Filter 63 A / D converter 64 data Processor 70 Substrate 71 Sub-scanning pulse motor 72 Pair of rails 73 Movable substrate 74 Rod 75 Main-scanning stepping motor 76 Endless belt 77 Linear encoder 78 Linear encoder slit 80 Control unit 81 Keyboard 82 Filter unit motor 91 Cooling CCD camera 92 Dark box 93 Personal computer 94 CRT 95 Keyboard 96 CCD 97 Heat transfer plate 98 Peltier element 99 Shutter 100 A / D converter 101 Data buffer 102 Turtle LA control circuit 105 Glass plate 106 Radiating fin 107 Camera lens 110 LED light source 111 Filter 112 Filter 113 Diffusion plate 120 CPU 121 Data transfer means 122 Data storage means 123 Data processing means 124 Data display means 125 Light source control means

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) // C12M 1/00 C12Q 1/68 A C12N 15/09 G01N 33/53 M C12Q 1/68 35/06 AG01N 33/53 C12N 15/00 FF term (reference) 2G045 DA13 FA12 FB02 FB05 FB08 FB12 GC15 JA01 2G058 AA09 CC02 EA11 GA02 GD07 HA00 4B024 AA11 AA19 CA04 CA09 HA14 4B029 AA01 AA23 QRBQ FA12 FA41 QBQ FAQR QS36 QS39 QX02

Claims (5)

[Claims] 1. A reaction vessel containing a biochemical analysis unit in which a plurality of absorptive regions containing a receptor or a ligand are formed separately from each other, and a reaction solution containing the ligand or the receptor labeled with a labeling substance is contained. Inside, a plurality of linear electrodes forming the first electrode group and a plurality of linear electrodes forming the second electrode group correspond to the plurality of absorptive regions of the biochemical analysis unit. At the position, so as to intersect, the first electrode group arranged in the reaction vessel, and the second electrode group is set between the plurality of line-shaped to configure the first electrode group. A positive or negative voltage is applied to at least one line electrode of the electrodes, and a negative voltage is applied to at least one line electrode of the plurality of line electrodes forming the second electrode group. Or by applying a positive voltage, the ligand or receptor contained in the reaction solution housed in the reaction container, so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, While forcibly moving, a negative or positive voltage is applied to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes forming the first electrode group, A positive or negative voltage is applied to at least one line-shaped electrode different from the at least one line-shaped electrode of the plurality of line-shaped electrodes that form the second electrode group, and is accommodated in the reaction container. Across the plurality of absorptive regions formed in the biochemical analysis unit, the ligand or receptor contained in the prepared reaction solution. As described above, the step of forcibly moving is repeated to selectively react the ligands or receptors contained in the reaction solution with the receptors or ligands contained in the plurality of absorptive regions of the biochemical analysis unit. And a method for associating a receptor / ligand. 2. The plurality of line-shaped electrodes constituting the first electrode group are applied with a positive or negative voltage to two or more line-shaped electrodes, and the plurality of line-shaped electrodes constitute the second electrode group. A negative or positive voltage is applied to two or more line-shaped electrodes of the above-mentioned line-shaped electrode, and the ligand or receptor contained in the reaction solution housed in the reaction vessel is transferred to the biochemical analysis unit. Two or more lines different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes that are forcibly moved so as to traverse the formed plurality of absorptive regions and form the first electrode group. A negative or positive voltage is applied to the linear electrodes, and two or more line-shaped electrodes different from the two or more line-shaped electrodes of the plurality of line-shaped electrodes forming the second electrode group are applied. By applying a positive or negative voltage to the electrode, the ligand or receptor contained in the reaction solution housed in the reaction vessel is transferred to the plurality of absorptive regions formed in the biochemical analysis unit. Repeat the step of forcibly moving it so that it crosses,
The ligand or receptor contained in the reaction solution is selectively associated with the receptor or ligand contained in the plurality of absorptive regions of the biochemical analysis unit. Receptor-ligand association reaction method of. 3. A positive or negative voltage is applied to two or more line-shaped electrodes of the plurality of line-shaped electrodes which are not adjacent to each other and which constitute the first electrode group, and the second electrode group is applied. A negative or positive voltage is applied to two or more line-shaped electrodes of the plurality of line-shaped electrodes that are not adjacent to each other to form a ligand contained in the reaction solution housed in the reaction container or The receptor is forcibly moved so as to cross the plurality of absorptive regions formed in the biochemical analysis unit, and the two or more of the plurality of line-shaped electrodes constituting the first electrode group are arranged. A negative or positive voltage is applied to two or more line-shaped electrodes that are different from the line-shaped electrodes and are not adjacent to each other, and the plurality of the line-shaped electrodes that constitute the second electrode group are applied. A positive or negative voltage is applied to two or more line-shaped electrodes of the line-shaped electrode which are different from the above-mentioned two or more line-shaped electrodes and are not included in the reaction solution contained in the reaction container. The ligand or receptor present in the biochemical analysis unit is forced to move across the plurality of absorptive regions formed in the biochemical analysis unit, and the plurality of absorptive properties of the biochemical analysis unit are repeated. 3. The receptor-ligand association reaction method according to claim 2, wherein the ligand or receptor contained in the reaction solution is selectively associated with the receptor or ligand contained in the region. 4. The biochemical analysis unit comprises a substrate having a plurality of holes formed therein, and each of the plurality of absorptive regions is filled with an absorptive material in the holes formed in the substrate. The receptor-ligand association reaction method according to claim 1, wherein the receptor-ligand association reaction method is carried out. 5. A reaction container comprising a biochemical analysis unit holding section for holding biochemical analysis units formed by separating a plurality of absorptive regions containing a receptor or a ligand, the reaction container Is provided with a first electrode group consisting of a plurality of linear electrodes on one side of the biochemical analysis unit holding portion, and a plurality of linear shapes on the other side of the biochemical analysis unit holding portion. A plurality of line-shaped electrodes comprising a second electrode group consisting of electrodes, the plurality of line-shaped electrodes constituting the first electrode group, and the plurality of line-shaped electrodes constituting the second electrode group are for the biochemical analysis. At positions corresponding to the plurality of absorptive regions of the biochemical analysis unit held in the unit holding portion, so as to intersect,
A reactor characterized by being placed.