JP2003004637A - Unit for biochemical analysis and its manufacturing method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a unit for biochemical analysis which can prevent noises resulting from scattering of electron beams (β rays) emitted from a radioactive marker substance from being generated in data for biochemical analysis even when the unit for biochemical analysis obtained by dropping a specific bond substance having known base sequence, base length, base composition and the like into spots to a carrier, forming high-density spot regions, specifically bonding a substance originating from a living body labeled by the radioactive marker substance to the spotted specific bond substance and selectively labeling the bond substance is tightly adhered to a photostimulable phosphor layer to expose the layer by the radioactive marker substance, and an accelerated phosphorescence emitted when the photostimulable phosphor layer is illuminated with an excitation light is photoelectrically detected, thereby generating the data for biochemical analysis to analyze the substance originating from the living body. SOLUTION: The unit for biochemical analysis has a plate-shaped member buried in an adsorptive film formed of an adsorptive material, The plate-shaped member formed of a material which attenuates radiation or light has a plurality of through holes. A plurality of adsorptive regions are formed to positions corresponding to the plurality of through holes.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a biochemical analysis unit and a method for producing the same, more specifically, it is capable of specifically binding to a substance of biological origin, and
A specific binding substance with a known base sequence, base length, composition, etc. is spotted on a carrier to form a high-density spot-shaped region, and the spot-shaped specific binding substance is radioactively labeled. A biochemical analysis unit obtained by selectively binding a substance derived from a living body labeled with a substance and selectively labeling it is brought into close contact with the stimulable phosphor layer to form a stimulable phosphor layer. Exposing with a radioactive labeling substance, irradiating the photostimulable phosphor layer with excitation light, photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor layer, and generating data for biochemical analysis. ,
Even when analyzing a substance derived from a living body, it is possible to prevent generation of noise in the data for biochemical analysis, which is caused by scattering of electron beams (β rays) emitted from the radiolabeled substance. Can specifically bind to the substance, and
A specific binding substance with a known base sequence, base length, composition, etc. is spotted on a carrier to form a high-density spot-shaped region, and the spot-shaped specific binding substance is radioactively labeled. In addition to the substance, or in place of the radiolabeled substance, a substance derived from a living body labeled with a labeling substance and / or a fluorescent substance, which causes chemiluminescence by contacting with a chemiluminescent substrate, is specifically bound, When the chemiluminescence and / or fluorescence emitted from the biochemical analysis unit obtained by selectively labeling are photoelectrically detected to generate biochemical analysis data and analyze a substance derived from a living body, Noise caused by scattering of chemiluminescence and / or fluorescence emitted from a labeling substance and / or a fluorescent substance which generate chemiluminescence when brought into contact with a chemiluminescent substrate is a biochemical solution. It relates the biochemical analysis unit and a manufacturing method thereof can be prevented from being generated during use data.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Further, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0007] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0008]

However, in the macroarray analysis system using the radiolabeled substance, when the stimulable phosphor layer is exposed by the radiolabeled substance, the surface of the carrier such as a membrane filter is exposed. Since the radioactive energy of the radiolabeled substance contained in the formed spot is very large, the electron beam (β-ray) emitted from the radiolabeled substance is scattered in the carrier such as the membrane filter and included in the adjacent spots. Incident on the region of the stimulable phosphor layer to be exposed by the radioactive labeling substance, or the electron beam emitted from the radioactive labeling substance is scattered,
Electrons emitted from radiolabeled substances contained in adjacent spots are mixed and incident on the region of the photostimulable phosphor layer, and as a result, biochemistry generated by photoelectrically detecting photostimulable light. When noise is generated in the analysis data and the radiation dose of each spot is quantified to analyze a substance derived from a living body, there is a problem that the quantitativeness deteriorates. In particular, a remarkable deterioration of the quantification was observed when trying to realize the same.

In order to prevent the noise caused by the scattering of the electron beam emitted from the radio-labeled substance contained in the adjacent spots and solve this problem, the distance between the adjacent spots must be increased. You will need to
There is a problem that the density of the spots is lowered and the inspection efficiency is lowered.

Furthermore, as described above, in the field of biochemical analysis, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, which are formed in spots at different positions on the surface of a carrier such as a membrane filter, Specific binding substances that can specifically bind to substances of biological origin, such as other proteins, nucleic acids, cDNA, DNA, RNA, etc., and have known base sequences, base lengths, compositions, etc., and radiolabeled substances. In addition, a substance derived from a living body that is labeled with a labeling substance and / or a fluorescent substance that produces chemiluminescence when brought into contact with a chemiluminescent substrate is specifically bound by hybridization or the like, and selectively labeled. , After exposing the stimulable phosphor layer with a radioactive labeling substance, or prior to exposing the stimulable phosphor layer with a radioactive labeling substance. Then, the chemiluminescent substrate is brought into contact with the chemiluminescent substrate, the chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeling substance is photoelectrically detected, and / or the excitation light is irradiated to the fluorescent substance. It is also required to photoelectrically detect the fluorescence emitted from a living body to analyze a substance derived from a living body, but in such a case, chemiluminescence or fluorescence emitted from a spot is scattered in a carrier such as a membrane filter. Alternatively, chemiluminescence or fluorescence emitted from a spot is scattered and mixed with chemiluminescence or fluorescence emitted from an adjacent spot, and as a result, chemiluminescence and / or fluorescence is photoelectrically detected and generated. There is a problem that noise is generated in the biochemical analysis data.

Therefore, according to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. is dropped onto a carrier in a spot form. To form a high-density spot-shaped region, and specifically bind the spot-specific binding substance with a biologically-derived substance labeled with a radiolabeling substance, and selectively label it The analysis unit is brought into close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed to a radioactive labeling substance, and the stimulable phosphor layer is irradiated with excitation light to produce a stimulable phosphor layer. The photostimulable light emitted from the photoelectron is detected photoelectrically to generate data for biochemical analysis, and even when a substance derived from a living body is analyzed, an electron beam (β
It is an object of the present invention to provide a biochemical analysis unit capable of preventing generation of noise in the biochemical analysis data due to scattering of (rays) and a manufacturing method thereof.

Another object of the present invention is to spot a specific binding substance, which is capable of specifically binding to a substance derived from a living body and whose base sequence, base length, composition, etc. are known, on a carrier. By dripping, a high-density spot-shaped region is formed, and chemiluminescence is generated by contacting the spot-like specific binding substance with a chemiluminescent substrate in addition to the radiolabeling substance or in place of the radiolabeling substance. A substance derived from a living body, which is labeled with a labeling substance and / or a fluorescent substance to be generated, is specifically bound, and chemiluminescence and / or fluorescence emitted from a biochemical analysis unit obtained by selectively labeling is photoelectrically converted. Labeled substance and / or fluorescent substance that produces chemiluminescence by contacting with a chemiluminescent substrate even when a substance derived from a living body is analyzed by generating data for biochemical analysis Noise caused by chemiluminescence and / or scattering of the fluorescence emitted from the present invention is to provide a biochemical analysis unit and a manufacturing method thereof can be prevented from being generated during biochemical analysis data.

[0013]

The object of the present invention is to:
A plate-like member formed of a material that attenuates radiation and / or light and having a plurality of through holes is embedded in the absorptive film formed of the absorptive material, and a position corresponding to the plurality of through holes is embedded. In addition, the biochemical analysis unit is characterized in that a plurality of absorptive regions are formed.

According to the present invention, when the plate member is made of a material having a property of attenuating radiation,
A specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. is dropped onto a plurality of absorptive regions formed in the biochemical analysis unit. After adsorbing, the substance of biological origin labeled with a radioactive labeling substance is specifically bound to the specific binding substance adsorbed in the adsorbing region, and after selectively adsorbing a plurality of adsorbing regions , When the biochemical analysis unit is opposed to the stimulable phosphor layer and the stimulable phosphor layer is exposed to a radioactive labeling substance, it has a property of attenuating radiation around each absorptive region. Since there is a plate-shaped member formed of the material, the electron beam (β-ray) emitted from the radioactive labeling substance contained in each adsorptive region is scattered,
It is possible to reliably prevent the scattered electron beam (β-ray) from entering the region of the stimulable phosphor layer to be exposed by the radio-labeled substance emitted from the adjacent adsorptive region. , Irradiating the stimulable phosphor layer exposed by the radiolabeled substance with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate data for biochemical analysis. However, even when analyzing a substance derived from a living body, it is possible to effectively prevent generation of noise in the biochemical analysis data due to scattering of electron beams (β rays) emitted from the radiolabeled substance. Will be possible.

Further, according to the present invention, when the plate-shaped member is formed of a material having a property of attenuating light, the plate-shaped member can specifically bind to a substance derived from a living body and has a base sequence or a base sequence. A specific binding substance of known length, composition, etc. is dropped onto a plurality of absorptive regions formed in the biochemical analysis unit to be adsorbed and contacted with a chemiluminescent substrate instead of the radiolabeled substance. Select a plurality of absorptive regions by specifically binding a biological substance labeled with a labeling substance and / or a fluorescent substance that causes chemiluminescence by the specific binding substance adsorbed in the absorptive region After being labeled chemically, it is contacted with a chemiluminescent substrate and irradiated with chemiluminescence emitted from the labeling substance and / or excitation light, and the fluorescence emitted from the fluorescent substance is photoelectrically detected to obtain biochemistry. analysis When generating data, a plate-shaped member made of a material having a property of attenuating light is present around each adsorptive region, so that chemiluminescence and / or fluorescence are reliably prevented from scattering. Therefore, it is effective to generate noise due to scattering of chemiluminescence and / or fluorescence in the data for biochemical analysis generated by photoelectrically detecting chemiluminescence and / or fluorescence. It becomes possible to prevent it.

Further, according to the present invention, when the plate-shaped member is formed of a material having a property of attenuating radiation and light, the plate-shaped member can specifically bind to a substance of biological origin and has a base sequence or Specific binding substances with known base length, composition, etc. are dropped onto multiple absorptive regions formed in the biochemical analysis unit to be adsorbed, and in addition to radiolabeled substances, contact with chemiluminescent substrate A substance of biological origin, which is labeled with a labeling substance and / or a fluorescent substance, which causes chemiluminescence by being caused to specifically bind to a specific binding substance adsorbed in the adsorbing region, thereby providing a plurality of adsorbing regions. After selectively labeling the photostimulable phosphor layer, and when exposing the photostimulable phosphor layer with a radioactive labeling substance, the radiation and light are attenuated around each absorptive region. Nature Since there is a plate-shaped member formed by the material that absorbs, the electron beam (β-ray) emitted from the radiolabeled substance contained in each adsorptive region is scattered, and the radioactivity emitted from the adjacent adsorptive regions is scattered. It is possible to reliably prevent the scattered electron beam (β-ray) from entering the region of the stimulable phosphor layer to be exposed by the labeling substance, and therefore, the stimulus exposed by the radioactive labeling substance. When irradiating the stimulable phosphor layer with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate biochemical analysis data and analyze a substance derived from a living body In addition, it is possible to effectively prevent the noise caused by the scattering of the electron beam (β-ray) emitted from the radiolabeled substance in the data for biochemical analysis, while Specific binding of substances to specific binding substances Then, the plurality of adsorptive regions are selectively labeled and then contacted with a chemiluminescent substrate to irradiate the chemiluminescence emitted from the labeling substance and / or excitation light to emit fluorescence from the fluorescent substance. Is detected photoelectrically, and when generating data for biochemical analysis, a plate-shaped member formed of a material having a property of attenuating radiation and light is present around each absorptive region, It is possible to reliably prevent the chemiluminescence and / or fluorescence from scattering, and therefore, the chemiluminescence and / or fluorescence is included in the data for biochemical analysis generated by photoelectrically detecting the chemiluminescence and / or fluorescence. It is possible to effectively prevent the generation of noise due to the scattering of the.

The above object of the present invention is also to embed a plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through-holes, in an adsorptive film formed of an adsorbent material. A method for manufacturing a biochemical analysis unit is characterized in that a plurality of absorptive regions are formed at positions corresponding to the plurality of through holes.

The object of the present invention is also to embed a plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through holes, in an adsorptive film formed of an adsorbent material. Then, a plurality of absorptive regions are formed at positions corresponding to the plurality of through-holes, and a specific binding substance having a known structure or property is dropped onto the plurality of absorptive regions to be adsorbed, and a radioactive label is provided. A substance derived from a living body that is labeled with at least one labeling substance selected from the group consisting of a labeling substance that produces chemiluminescence when brought into contact with a substance, a fluorescent substance, and a chemiluminescent substrate is adsorbed to the plurality of absorptive regions. The biochemical analysis unit is characterized in that the plurality of absorptive regions are selectively labeled by being specifically bound to the specific binding substance that is present. It is achieved.

According to the present invention, the absorptive film formed of the absorptive material is embedded with a plate-like member formed of a material that attenuates radiation and / or light and having a plurality of through holes, A plurality of absorptive regions are formed at positions corresponding to a plurality of through-holes, and a specific binding substance having a known structure or property is dropped onto the plurality of absorptive regions and is adsorbed to a radiolabeled substance or a fluorescent substance. And a substance derived from a living body that is labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a chemiluminescent substrate is specifically adsorbed on a plurality of absorptive regions. When a plurality of absorptive regions are selectively labeled by being specifically bound to a binding substance, the plate-shaped member is formed of a material having a property of attenuating radiation. Is a property of attenuating radiation around each absorptive region when the biochemical analysis unit is opposed to the stimulable phosphor layer and the stimulable phosphor layer is exposed to a radioactive labeling substance. There is a plate-like member formed of a material having, therefore, an electron beam (β-ray) emitted from the radiolabeled substance contained in each adsorptive region.
Reliably prevents the scattered electron beam (β-ray) from entering the region of the stimulable phosphor layer to be exposed by the radioactive labeling substance emitted from the adjacent adsorbing region. Therefore, it is possible to irradiate the photostimulable phosphor layer exposed by the radiolabeled substance with excitation light, and photoelectrically detect the photostimulable light emitted from the photostimulable phosphor layer,
When generating data for biochemical analysis and analyzing substances of biological origin, the electron beam (β
It is possible to effectively prevent the noise caused by the scattering of the (rays) from being generated in the biochemical analysis data.

Further, according to the present invention, when the plate-like member is formed of a material having a property of attenuating light, the plate-shaped member is brought into contact with a chemiluminescent substrate, and the visible light generated by the contact between the chemiluminescent substrate and the labeling substance. When the chemiluminescence in the light wavelength region is photoelectrically detected and / or the excitation light is irradiated to detect the fluorescence emitted from the fluorescent substance photoelectrically to generate biochemical analysis data, Since a plate-like member formed of a material having a property of attenuating light is present around each adsorptive region of the analysis unit, chemiluminescence and / or fluorescence emitted from each adsorptive region is scattered. Therefore, in the data for biochemical analysis generated by photoelectrically detecting chemiluminescence and / or fluorescence, the data resulting from the scattering of chemiluminescence and / or fluorescence is detected. 'S it becomes possible to effectively prevented from being generated.

Further, according to the present invention, when the plate member is made of a material having a property of attenuating radiation and light, the biochemical analysis unit is made to face the stimulable phosphor layer, When the stimulable phosphor layer is exposed to a radioactive labeling substance, a plate-like member formed of a material having a property of attenuating radiation and light is present around each adsorptive region, and therefore each adsorption In the region of the stimulable phosphor layer to be exposed by the radiolabeled substance emitted from the adsorbing region adjacent to the electron beam emitted from the radiolabeled substance contained in the absorptive region is scattered. Since it is possible to reliably prevent the scattered electron beam (β-ray) from entering the photo-stimulable phosphor layer, the photo-stimulable phosphor layer exposed by the radio-labeled substance is irradiated with excitation light to stimulate photo-stimulation. Emitted from the phosphor layer When photostimulated photostimulable light is generated photoelectrically to generate data for biochemical analysis and a substance derived from a living body is analyzed, an electron beam (β
It is possible to effectively prevent the generation of noise in the data for biochemical analysis due to the scattering of the (rays), and to make the chemiluminescence emitted from the labeling substance contact with the chemiluminescence substrate. When photoelectrically detecting and / or irradiating with excitation light, the fluorescence emitted from the fluorescent substance is photoelectrically detected to generate data for biochemical analysis. The presence of the plate-shaped member formed of a material having a property of attenuating radiation and light can reliably prevent the chemiluminescence and / or fluorescence emitted from each adsorptive region from being scattered, and , Effectively prevent generation of noise due to chemiluminescence and / or fluorescence scattering in biochemical analysis data generated by photoelectrically detecting chemiluminescence and / or fluorescence Door is possible.

The object of the present invention is also to embed a plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through-holes, in an adsorptive film formed of an adsorbent material. , At a position corresponding to the plurality of through-holes, a plurality of absorptive regions are formed, the plurality of absorptive regions are dropped by a specific binding substance having a known structure or property, and adsorbed, and a radiolabeled substance, A substance derived from a living body labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a fluorescent substance and a chemiluminescent substrate is adsorbed to the plurality of absorptive regions; It is achieved by a method for producing a unit for biochemical analysis, which comprises specifically binding to the specific binding substance present and selectively labeling the plurality of absorptive regions. It is.

[0023] In a preferred aspect of the present invention, the substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor ligand.

In a preferred aspect of the present invention, the plate-shaped member is press-fitted into the absorptive film to form the plurality of absorptive regions.

According to a preferred embodiment of the present invention, a plurality of absorptive regions can be formed by simply press-fitting the plate-shaped member into the absorptive membrane, so that the biochemical analysis unit can be simply and easily formed. And will be able to generate.

[0026] In a further preferred aspect of the present invention, the plate-shaped member is press-fitted into the adsorptive membrane by a hot press treatment to form the plurality of porous regions.

According to a further preferred embodiment of the present invention, a plurality of absorptive regions can be formed simply by press-fitting the plate-like member into the absorptive film by hot pressing. Moreover, it is possible to generate the biochemical analysis unit at a low cost.

[0028] In a further preferred aspect of the present invention, the plate-shaped member is subjected to calender roll treatment.
The plurality of porous regions are formed by being pressed into the adsorptive membrane.

According to a further preferred embodiment of the present invention, a plurality of absorptive regions can be formed simply by press-fitting the plate-shaped member into the absorptive film by calender roll treatment. Moreover, it is possible to generate the biochemical analysis unit at a low cost.

In a further preferred aspect of the present invention, the plate-shaped member is pressed into the adsorptive film via an adhesive to form the plurality of adsorptive regions.

According to a further preferred embodiment of the present invention, the plate member is press-fitted into the adsorptive film via an adhesive,
Since the plurality of absorptive regions are formed, it becomes possible to firmly integrate the plate-shaped member and the absorptive film.

In a preferred embodiment of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions, It has the property of attenuating the energy of radiation and / or light to 1/5 or less.

In a further preferred aspect of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions. , And has the property of attenuating the energy of radiation and / or light to 1/10 or less.

[0034] In a further preferred aspect of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to a distance between the adjacent absorptive regions. , And has the property of attenuating the energy of radiation and / or light to 1/50 or less.

In a further preferred aspect of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions. , And has the property of attenuating the energy of radiation and / or light to 1/100 or less.

In a further preferred aspect of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions. , And has the property of attenuating the energy of radiation and / or light to 1/500 or less.

In a further preferred aspect of the present invention, when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions. , And has the property of attenuating the energy of radiation and / or light to 1/100 or less.

In the present invention, the material for forming the plate-like member is not particularly limited as long as it has a property of attenuating radiation and / or light, and is not limited to inorganic compound materials and organic compound materials. Although any of the above can be used, a metal material, a ceramic material or a plastic material is preferably used.

In the present invention, examples of the inorganic compound material capable of attenuating radiation and / or light which can be preferably used for forming the plate-shaped member include:
Gold, silver, copper, zinc, aluminum, titanium, tantalum,
Metals such as chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; aluminum oxide, magnesium oxide , Metal oxides such as zirconium oxide;
Inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material capable of attenuating the radiation and / or the light, and the radiation and / or the light which can be preferably used for forming the plate member is used. As the high molecular weight compound that can be attenuated, for example,
Polyolefins such as polyethylene and polypropylene;
Acrylic resins such as polymethylmethacrylate and butylacrylate / methylmethacrylate copolymer; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyethylene naphthalate and polyethylene Polyester such as terephthalate; nylon 6, nylon 6,
6, nylon such as nylon 4, 10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenylsiloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer; cellulose, cellulose acetate, Examples thereof include polysaccharides such as nitrocellulose, starch, calcium alginate and hydroxypropylmethyl cellulose; chitin; chitosan; sumacum; polyamides such as gelatin, collagen and keratin, and copolymers of these polymer compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the larger the specific gravity is, the higher the radiation attenuating ability is. Therefore, in the present invention, when the plate member is made of a material having the property of attenuating the radiation, the specific gravity is 1.0 g / cm ³ or more. Preferably, it is formed of a compound material or a composite material of
It is particularly preferable that it is formed of a compound material or a composite material of g / cm ³ or more and 23 g / cm ³ or less.

On the other hand, in general, the greater the scattering and / or absorption of light, the higher the light attenuating ability. Therefore, in the present invention, when the plate member is made of a material having a property of attenuating light, The absorbance per cm of thickness is preferably 0.3 or more, and more preferably the absorbance per cm of thickness is 1 or more. Here, for the absorbance, an integrating sphere is placed immediately after the plate body having a thickness of Tcm,
The transmitted light amount A at the wavelength of the probe light or the emission light used for measurement is measured by a spectrophotometer, and A /
It is obtained by calculating T.

In the present invention, in order to improve the light attenuating ability, a light-scattering body or a light-absorbing body may be contained in the plate member. As the light scatterer, fine particles of a material different from the material forming the substrate and the porous plate of the biochemical analysis unit are used, and as the light absorber, a pigment or a dye is used.

In a preferred embodiment of the present invention, the plate member is formed by dispersing metal oxide particles in a plastic material.

The object of the present invention is also that the adsorbent film formed of the adsorbent material is impregnated with a solution containing colloidal metal particles to form a shield region that attenuates radiation and light. The biochemical analysis unit is characterized in that a plurality of absorptive regions are formed by the region in which the shielding region is not formed.

According to the present invention, the adsorptive film formed of the adsorptive material is impregnated with the solution containing the particles of the metal colloid to form the shielding region for attenuating the radiation and the light. Since a plurality of absorptive areas are formed by the area where the shielding area is not formed,
A specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. is dropped onto a plurality of absorptive regions formed in the biochemical analysis unit. After adsorbing, the substance of biological origin labeled with a radioactive labeling substance is specifically bound to the specific binding substance adsorbed in the adsorbing region, and after selectively adsorbing a plurality of adsorbing regions , When the biochemical analysis unit is opposed to the stimulable phosphor layer and the stimulable phosphor layer is exposed to a radioactive labeling substance, a solution containing metal colloid simple particles around each adsorptive region. However, there is a shielding member formed of a material having a property of attenuating the adsorptive film and attenuating the formed radiation and light, and therefore, the radiation member emitted from the radio-labeled substance contained in each adsorptive region is present. Electron beam (β ray) Be sure to prevent scattered electron beams (β-rays) from entering the region of the stimulable phosphor layer that is scattered and exposed by the radioactive labeling substance emitted from the adjacent adsorptive region. Therefore, it is possible to irradiate the photostimulable phosphor layer exposed by the radiolabeled substance with excitation light, and photoelectrically detect the photostimulable light emitted from the photostimulable phosphor layer. When generating analysis data and analyzing substances of biological origin, it is effective to generate noise in the biochemical analysis data due to scattering of electron beams (β rays) emitted from radiolabeled substances. Specific binding substance that can be specifically prevented and that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known as a biochemical analysis unit. Drip onto the formed multiple absorptive regions to absorb The specific substance adsorbed in the adsorptive region is a biological substance labeled with a labeling substance and / or a fluorescent substance that causes chemiluminescence by contacting with a chemiluminescent substrate instead of the radioactive labeling substance. After specifically binding to a substance and selectively labeling a plurality of adsorptive regions, the substance is brought into contact with a chemiluminescent substrate and irradiated with chemiluminescence and / or excitation light emitted from the labeled substance, When photoelectrically detecting fluorescence emitted from a fluorescent substance to generate data for biochemical analysis, a solution containing colloidal particles of metal alone is impregnated into the adsorbent film around each adsorbent region. ,
There is a shielding member formed of a material that has the property of attenuating the formed radiation and light, and thus
Since it is possible to reliably prevent the chemiluminescence and / or fluorescence emitted from each adsorptive region from being scattered, therefore, the biochemical analysis generated by photoelectrically detecting the chemiluminescence and / or fluorescence. It is possible to effectively prevent generation of noise in the usage data due to chemiluminescence and / or fluorescence scattering.

The above object of the present invention is also to impregnate an adsorptive film formed of an adsorptive material with a solution containing colloidal particles of a simple metal to form a shielding region for attenuating radiation and light, thereby providing the adsorptive property. This is achieved by a method for manufacturing a biochemical analysis unit, characterized in that a plurality of absorptive regions are formed by regions of the membrane where the shielding regions are not formed.

The object of the present invention is also that the adsorbent film formed of the adsorbent material is impregnated with a solution containing colloidal metal particles to form a shield region for attenuating radiation and light. A plurality of absorptive regions are formed by the region where the shielding region of is not formed,
In the plurality of adsorptive regions, a specific binding substance having a known structure or property is dropped and adsorbed, and a radiolabeled substance,
When a substance derived from a living body labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a fluorescent substance and a chemiluminescent substrate is adsorbed to the plurality of absorptive regions, This is achieved by a unit for biochemical analysis, characterized in that the plurality of absorptive regions are selectively labeled by being specifically bound to the specific binding substance present.

According to the present invention, the adsorptive film formed of the adsorptive material is impregnated with the solution containing colloidal metal particles to form a shield region for attenuating the radiation and the light, thereby shielding the adsorptive film. A plurality of absorptive regions are formed by the regions where the regions are not formed, and a specific binding substance having a known structure or property is dropped onto the plurality of absorptive regions and is adsorbed to the radioactive labeling substance, the fluorescent substance, and the Specific binding in which a biological substance labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a chemiluminescent substrate is adsorbed to a plurality of absorptive regions When a substance derived from a living body is labeled with a radiolabeled substance, it is specifically bound to the substance and the multiple absorptive regions are selectively labeled. , The biochemical analysis unit is opposed to the stimulable phosphor layer, and when the stimulable phosphor layer is exposed to a radioactive labeling substance, a solution containing metal colloid particles is placed around each adsorptive region. , There is a shielding member formed of a material having a property of attenuating the adsorptive film and attenuating the formed radiation and light, and thus, the electrons emitted from the radio-labeled substance contained in each adsorptive region are present. Rays (β-rays) are scattered and the scattered electron rays (β-rays) are incident on the region of the stimulable phosphor layer to be exposed by the radio-labeled substance emitted from the adjacent adsorptive region. Therefore, it is possible to reliably prevent the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, photoelectrically stimulated photostimulable light emitted from the photostimulable phosphor layer. Detect and generate data for biochemical analysis However, even when analyzing a substance derived from a living body, it is possible to effectively prevent generation of noise in the biochemical analysis data due to scattering of electron beams (β rays) emitted from the radiolabeled substance. When the substance derived from the living body is labeled with a labeling substance that causes chemiluminescence by contacting with a fluorescent substance and / or a chemiluminescent substrate, the substance is brought into contact with the chemiluminescent substrate to form a chemiluminescent substrate. The chemiluminescence in the visible light wavelength range generated by the contact between the fluorescent substance and the labeling substance is photoelectrically detected, and / or the excitation light is irradiated, and the fluorescence emitted from the fluorescent substance is photoelectrically detected to perform biochemical analysis. Around each absorptive area when generating data for
A solution containing colloidal metal particles is impregnated into the adsorptive film, and there is a shielding member formed of a material having a property of attenuating the formed radiation and light, and thus released from each of the adsorptive regions. Since it is possible to reliably prevent the chemiluminescence and / or fluorescence from scattering, the chemiluminescence and / or fluorescence may be included in the biochemical analysis data generated by photoelectrically detecting the chemiluminescence and / or fluorescence. It is possible to effectively prevent the generation of noise due to the scattering of fluorescence.

The object of the present invention is also to impregnate an adsorptive film formed of an adsorptive material with a solution containing colloidal particles of a metal simple substance to form a shielding region for attenuating radiation and light, thereby providing the adsorptive property. A plurality of absorptive regions are formed by the region where the shielding region of the film is not formed, and a specific binding substance having a known structure or property is dropped onto the plurality of absorptive regions and is adsorbed to the radioactive labeling substance. A substance derived from a living body, which is labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a fluorescent substance and a chemiluminescent substrate, is adsorbed to the plurality of absorptive regions. The method for producing a unit for biochemical analysis, characterized in that the plurality of absorptive regions are selectively labeled by specifically binding to the specific binding substance Thus it is achieved.

In a preferred embodiment of the present invention, the substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, antigen-antibody reaction, and receptor-ligand.

In the present invention, the metallic material forming the simple metal colloidal particles is not particularly limited as long as it has a property of attenuating radiation and light, and examples thereof include gold, silver, platinum, zinc, Examples thereof include metals such as aluminum, titanium, tungsten, chromium, iron, nickel, cadmium, mercury and lead, and alloys thereof such as stainless steel.

In a preferred embodiment of the present invention, 10 or more absorptive regions are formed on the absorptive film.

In a further preferred aspect of the present invention, 100 or more absorptive regions are formed in the absorptive film.

In a further preferred aspect of the present invention, the absorptive film is formed with 1000 or more absorptive regions.

[0056] In a further preferred aspect of the present invention, the absorptive film is formed with 10,000 or more absorptive regions.

In a further preferred aspect of the present invention, 100,000 or more absorptive regions are formed in the absorptive film.

In a preferred aspect of the present invention, each of the plurality of absorptive regions is formed to have a size of less than 5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed to have a size of less than 1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed to have a size of less than 0.5 mm 2.

[0061] In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed so as to have a size of less than 0.1 mm 2.

[0062] In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed so as to have a size of less than 0.05 mm 2.

[0063] In a further preferred aspect of the present invention, each of the plurality of absorptive regions is formed so as to have a size of less than 0.01 mm 2.

In a preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 10 pieces / square centimeter or more.

[0065] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 50 or more per cm 2.

[0066] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 100 pieces / square centimeter or more.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 500 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 1,000 or more per cm 2.

[0069] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 5,000 or more per cm 2.

[0070] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed at a density of 10,000 or more per cm 2.

In a preferred embodiment of the present invention, the absorptive regions are regularly formed.

In the present invention, a porous material or a fiber material is preferably used as the absorptive material forming the absorptive region. The porous material and the fiber material may be used together to form the adsorptive region.

In the present invention, the porous material used for forming the adsorptive region may be either an organic material or an inorganic material, or an organic / inorganic composite.

In the present invention, the organic porous material used to form the adsorptive region is not particularly limited, but a carbon porous material such as activated carbon or a porous material capable of forming a membrane filter. Are preferably used. Specifically, nylon 6, nylon 6,
6, nylons such as nylon 4, 10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid such as alginic acid, calcium alginate, alginic acid / polylysine polyion complex; polyolefins such as polyethylene and polypropylene; Examples thereof include polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluoride such as polytetrafluoride, and copolymers or composites thereof.

In the present invention, the inorganic porous material used for forming the adsorptive region is not particularly limited, but preferably, for example, platinum, gold, iron,
Examples thereof include metals such as silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fiber material used for forming the absorptive region is not particularly limited, but preferably, for example, nylon 6, nylon 6,6, nylon 4,10 and the like. Examples include nylons, cellulose derivatives such as nitrocellulose, cellulose acetate, and cellulose acetate butyrate.

[0077]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention, and FIG. 2 is a partial sectional view thereof.

As shown in FIGS. 1 and 2, the biochemical analysis unit 1 according to the present embodiment is provided with an adsorptive membrane 2 formed of nylon 6 capable of forming a membrane filter. Has a property of attenuating radiation and light, and an aluminum plate 5 in which a large number of substantially circular through holes 4 are regularly formed is press-fitted by a calendering device, whereby a large number of aluminum plates 5 are formed. A large number of absorptive regions 4 are regularly formed in a dot shape so as to correspond to the through holes 4. Here, the adhesive 3 is applied to the back surface of the aluminum plate 5, and the aluminum plate 5 is press-fitted into the adsorptive film 2 via the adhesive 3. Therefore, the aluminum plate 5 is It is firmly integrated with the 2 to improve the durability.

Although not shown exactly in FIG. 1, in the present embodiment there are approximately 5000 absorptive areas 4 of approximately circular shape having a size of approximately 0.01 mm 2 of approximately 10,000 / square centimeter. Density, regularly,
It is formed in the biochemical analysis unit 1.

As shown in FIG. 2, in this embodiment, the aluminum plate 5 is arranged so that the surface of the absorptive region 4 and the surface of the aluminum plate 5 are located at the same height.
The biochemical analysis unit 1 is formed by being pressed into the adsorptive membrane 2.

FIG. 3 is a schematic sectional view of a calendering apparatus for manufacturing the biochemical analysis unit 1.

The calendering apparatus comprises a pair of temperature-controlled calender rolls 15. The adsorbent film 2 and the aluminum plate 5 on the lower surface of which the adhesive 3 is applied are laminated to form a pair of calender rolls 15. During supply, the aluminum plate 5 is press-fitted into the adsorptive film 2 by the supply, and the through holes 4 are regularly formed in the aluminum plate 5.
Corresponding to, the biochemical analysis unit 1 in which a large number of absorptive regions 4 are regularly formed is manufactured.

FIG. 4 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 4, in a large number of absorptive regions 4 regularly formed in the biochemical analysis unit 1, for example, a base sequence as a specific binding substance is used. Multiple known different cDNAs
Are dropped using a spotting device.

As shown in FIG. 4, the spotting device comprises an injector 6 and a CCD camera 7, and a CCD
By the camera 7, the tip of the injector 6 and the cDN
While observing the absorptive region 4 to which A is to be dropped, the tip portion of the injector 6 and the absorptive region 4 to which the cDNA is to be dropped
From the injector 6, when the center of
It is configured so that NA is released and dropped into the adsorptive region 4, so that the cDNA can be accurately dropped into the large number of adsorptive regions 4 formed in the biochemical analysis unit 1. Guaranteed.

In the present embodiment, in the adsorptive region 4 of the biochemical analysis unit 1, the aluminum plate 5 in which a large number of through holes 4 are regularly formed is press-fitted into the adsorptive film 2,
Since it is formed, the holes in the adsorptive film 2 located between the adjacent adsorptive regions 4 have disappeared by the pressurization,
Therefore, the specific binding substance dropped in the absorptive region 4 is effectively prevented from diffusing into the region of the absorptive membrane 2 other than the absorptive region 4, and the specific binding substance dropped in the absorptive region 4 is effectively prevented. The binding substance is adsorbed only on the adsorptive region 4.

FIG. 5 is a schematic cross-sectional view of the hybridization container.

As shown in FIG. 5, the hybridization container 8 has a rectangular cross section, and contains a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with a labeling substance. .

In the case of selectively labeling a specific binding substance such as cDNA with a radioactive labeling substance, a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with a radioactive labeling substance is prepared and It is housed in the hybridization container 8.

On the other hand, by using a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate,
In the case of selectively labeling a specific binding substance such as, a hybridization solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, is prepared. And is housed in the hybridization container 8.

Furthermore, in the case of selectively labeling a specific binding substance such as cDNA with a fluorescent substance such as a fluorescent dye, a high-molecular substance containing a probe which is a probe labeled with a fluorescent substance such as a fluorescent dye is used. A hybridization solution 9 is prepared and housed in the hybridization container 8.

Living body labeled with a radioactive labeling substance, living body labeled with a labeling substance that produces chemiluminescence by contacting with a chemiluminescent substrate, and living body labeled with a fluorescent substance such as a fluorescent dye It is also possible to prepare a hybridization solution 9 containing two or more substances derived from the living body among the substances derived from the living body, and store the hybridization solution 9 in the hybridization container 8. In the present embodiment, the living body labeled with the radioactive labeling substance is used. A hybridization solution 9 containing a substance derived from a living body, a substance derived from a living body labeled with a fluorescent substance such as a fluorescent dye, and a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is prepared. And is accommodated in the hybridization container 8.

For hybridization, cD
The biochemical analysis unit 1 in which a specific binding substance such as NA is adsorbed on a large number of absorptive regions 4 is inserted into the hybridization container 8.

As a result, the specific binding substance adsorbed on the large number of adsorptive regions 4 is labeled with a radioactive labeling substance and is contained in the hybridization solution 9 by a biological substance or a fluorescent substance such as a fluorescent dye. The substance of biological origin that is labeled and labeled with the substance of biological origin contained in the hybridization solution 9 and the labeling substance that causes chemiluminescence by contacting with the chemiluminescent substrate are selectively hybridized.

Thus, a large number of absorptive regions 4 of the biochemical analysis unit 1 are brought into contact with the radiation data of the radioactive labeling substance as the labeling substance, the fluorescence data of the fluorescent substance such as a fluorescent dye, and the chemiluminescent substrate. The chemiluminescence data of the labeling substance that gives rise to luminescence is recorded. The fluorescence data recorded in the absorptive region 4 is read by a scanner described later to generate biochemical analysis data,
On the other hand, the chemiluminescence data recorded in the adsorptive area 4 is read by a cooling CCD camera of a data generation system described later, and biochemical analysis data is generated.

On the other hand, the radiation data of the radiolabeled substance is
The radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by a scanner described later to generate biochemical analysis data.

FIG. 6 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 6, the stimulable phosphor sheet 10 includes a support 11, and on one side of the support 11, a large number of absorptive regions formed in the biochemical analysis unit 1 are provided. A large number of concave portions 13 are formed in the same regular pattern as the pattern 4 and a stimulable phosphor is embedded in the large number of concave portions 13 to form a large number of substantially circular dot-shaped stimulable phosphor layers. Region 12 is formed.

In this embodiment, the support 11 is made of stainless steel having the property of attenuating radiation, and the large number of recesses 13, and thus the substantially circular dot-shaped stimulable phosphor layer region 12, are It is formed on the support 11 so as to have a diameter equal to that of the absorptive region 4 formed on the biochemical analysis unit 1.

Further, the dot-shaped stimulable phosphor layer region 12
The photostimulable phosphor is embedded in the recess 13 so that the surface of the substrate and the surface of the support 11 are located at the same height, and the dot-shaped photostimulable phosphor layer region 12 is formed. There is.

FIG. 7 shows a large number of dot-shaped stimulable fluorescent substances formed on the stimulable phosphor sheet 10 by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1. It is a schematic sectional drawing which shows the method of exposing a body layer area | region 12.

As shown in FIG. 7, upon exposure,
The many absorptive regions 4 formed in the biochemical analysis unit 1 face the many dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are superposed.

In the present embodiment, since the biochemical analysis unit 1 is formed by press-fitting the aluminum plate 5 into the adsorptive membrane 2, even if it is subjected to liquid treatment such as hybridization, Therefore, the many absorptive regions 4 formed in the biochemical analysis unit 1 do not expand and contract, and therefore a large number of dot-shaped stimulable phosphors are formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are easily and surely overlapped with each other so as to exactly face the layer region 12, and the dot-shaped stimulable phosphor layer region 12 is exposed. It will be possible.

Thus, over the predetermined time, each of the large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the biochemical analysis unit 1 are formed. By opposition to the large number of absorptive regions 4, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are formed by the radioactive labeling substance contained in the absorptive regions 4. Exposed.

At this time, an electron beam is emitted from the radio-labeled substance adsorbed in the adsorptive region 4, but the adsorptive region 4 of the biochemical analysis unit 1 has a large number of through holes 4 in the adsorptive film 2. The aluminum plate 5 on which is formed is press-fitted and formed, and since the aluminum plate 5 having the property of attenuating radiation exists around the adsorptive region 4, it is included in the adsorptive region 4. The electron beam emitted from the radiolabeled substance is surely prevented from scattering, and all the electron beams emitted from the radiolabeled substance contained in the adsorptive region 4 oppose the adsorptive region 4. Stimulable phosphor layer region 1 which is to be exposed to the electron beam emitted from the adsorbing region 4 adjacent to the stimulable phosphor layer region 12.
It is possible to surely prevent the light from entering the beam 2.

Therefore, the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are the only radiolabeled substances contained in the corresponding absorptive regions 4 of the biochemical analysis unit 1. This makes it possible to reliably perform exposure.

Thus, the radiation data of the radioactive labeling substance is recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10.

FIG. 8 shows a large number of dot-shaped stimulable phosphor layer regions 1 formed on the support 11 of the stimulable phosphor sheet 10.
An example of a scanner that reads the radiation data of the radiolabeled substance recorded in 2 and the fluorescence data of the fluorescent dyes recorded in the many absorptive regions 4 of the biochemical analysis unit 1 to generate biochemical analysis data FIG. 8 is a schematic perspective view showing the scanner, and FIG. 8 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner shown in FIG. 8 has the radiation data and biochemistry of the radiolabeled substance recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. The first laser excitation light source 21 that emits the laser light 24 having a wavelength of 640 nm and the first laser excitation light source 532n are configured to be able to read the fluorescence data such as the fluorescent dye recorded in the many absorptive regions 4 of the analysis unit 1.
A second laser excitation light source 22 which emits a laser beam 24 having a wavelength of m and a third laser which emits a laser beam 24 having a wavelength of 473 nm.
Laser excitation light source 23. In the present embodiment, the first laser pumping light source 21 is composed of a semiconductor laser light source, and the second laser pumping light source 22 and the third laser pumping light source 23 are second harmonic generation (Second).
Harmonic Generation) element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. First
In the optical path of the laser light 24 emitted from the laser excitation light source 21 and reflected by the mirror 26, the first dichroic mirror 27 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer A second dichroic mirror 28 that transmits light of a wavelength and reflects light of a wavelength of 473 nm is provided.
Laser light 24 generated by the laser excitation light source 21 of
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated from the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, it is reflected by the first dichroic mirror 27, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 28 and enters the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser light 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 or the biochemical analysis unit 1 placed on 41. In FIG. 7, the biochemical analysis unit 1 has a surface on which the aluminum plate 5 is press-fitted,
It is placed on the glass plate 41 of the stage 40 so as to face downward.

When the laser beam 24 is incident on the dot-shaped stimulable phosphor layer area 12 of the stimulable phosphor sheet 10, it is included in the dot-shaped stimulable phosphor layer area 12 formed on the stimulable phosphor sheet 10. When the stimulable phosphor present in the biochemical analysis unit 1 is excited to emit photostimulable light 45 and the laser light 24 is incident on the biochemical analysis unit 1, the adsorptive region 4 formed in the biochemical analysis unit 1 is detected. The contained fluorescent dye or the like is excited, and the fluorescence 45 is emitted.

The photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 of the stimulable phosphor sheet 10 or the fluorescence 45 emitted from the absorptive region 4 formed in the biochemical analysis unit 1 is The aspherical lens 37 provided in the optical head 35 collects the light on a mirror 36, reflects the light on the same side as the optical path of the laser light 24 by the mirror 36, converts the light into parallel light, and enters the concave mirror 38. .

The photostimulable light 45 or the fluorescent light 45 incident on the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror and enters the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 9, the filter unit 48 includes four filter members 51a, 51b, 51c,
51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 9 by a motor (not shown).

FIG. 10 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 10, the filter member 5
1a includes a filter 52a, and the filter 52a includes a first
Using the laser excitation light source 21 described above, a fluorescent material such as a fluorescent dye contained in the absorptive region 4 formed in the biochemical analysis unit 1 is excited to read the fluorescence 45. And has a property of cutting off light having a wavelength of 640 nm and transmitting light having a wavelength longer than 640 nm.

FIG. 11 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 11, the filter member 5
1b includes a filter 52b, and the filter 52b includes a second
Using the laser excitation light source 22 described above, a fluorescent material such as a fluorescent dye contained in the absorptive region 4 formed in the biochemical analysis unit 1 is excited, and a filter member used when reading the fluorescence 45. And has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 12 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 12, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
The laser excitation light source 23 is used to excite a fluorescent substance such as a fluorescent dye contained in the absorptive region 4 formed in the biochemical analysis unit 1, and a filter member used when reading the fluorescence 45. Therefore, it has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 13 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 13, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
It is used when the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 formed in 0 is excited to read the stimulable light 45 emitted from the stimulable phosphor layer 12. It is a filter and has a property of transmitting only light in the wavelength region of stimulable light emitted from the stimulable phosphor layer 12 and cutting light of a wavelength of 640 nm.

Therefore, depending on the laser excitation light source to be used, the filter members 51a, 51b, 51c, 51d.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

Although not shown in FIG. 8, the optical head 35 is configured to be movable in the X and Y directions in FIG. 8 by the scanning mechanism, and the stimulable phosphor sheet 1 is formed.
All the dot-shaped stimulable phosphor layer regions 1 formed in 0
2 or the entire surface of the biochemical analysis unit 1 is configured to be scanned by the laser beam 24.

FIG. 14 is a schematic plan view of the scanning mechanism of the optical head. In FIG. 14, for simplification, the optical system excluding the optical head 35 and the optical paths of the laser light 24 and the stimulated light 45 or the fluorescence 45 are omitted.

As shown in FIG. 14, the optical head 35
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
14 are fixed, and the substrate 6 which is movable on the substrate 60 in the sub-scanning direction indicated by the arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in this hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63, and the main scanning stepping motor 65 connects the endless belt 66 between adjacent adsorbing regions 4 formed in the biochemical analysis unit 1. Adjacent to the stimulable phosphor layer regions 1 formed on the support 11 of the stimulable phosphor sheet 10 at a pitch equal to the distance
It can be driven intermittently at a pitch equal to the distance between the two. The optical head 35 includes an endless belt 66.
When the endless belt 66 is driven by the main scanning stepping motor 65, the endless belt 66 is moved in the main scanning direction indicated by the arrow X in FIG.

In FIG. 14, 67 is the optical head 35.
Is a linear encoder for detecting the position in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is driven in the main scanning direction, and when the scanning of one line is completed, the substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61, whereby the optical head 35. 14 is moved in the X-Y direction in FIG. 14 and the entire surface of all the dot-shaped stimulable phosphor layer regions 12 or the biochemical analysis unit 1 formed on the stimulable phosphor sheet 10 by the laser light 24. Are scanned.

FIG. 15 is a block diagram showing the control system, input system, drive system and detection system of the scanner shown in FIG.

As shown in FIG. 15, the control system of the scanner is a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 that is operated by an operator and can input various instruction signals.

As shown in FIG. 15, the scanner drive system intermittently moves the optical head 35 in the main scanning direction, and a main scanning stepping motor 65, and intermittently moves the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

Further, as shown in FIG. 15, the detection system of the scanner includes a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser excitation light source 21, the second laser excitation light source 22 or the third laser excitation light source 21 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The scanner configured as described above uses a large number of dot-shaped dots by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 in the following manner. The stimulable phosphor layer region 12 is exposed to read the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 10 to generate biochemical analysis data.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40 so that a large number of dot-shaped stimulable phosphor layer regions 12 are in contact with the surface of the glass plate 41.

Next, the user operates the keyboard 7
An instruction signal for scanning a large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 with a laser beam 24 is input to the display device 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
The filter member 51d provided with the filter 52d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is positioned in the optical path of the stimulated emission light 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, A position where the first dot-shaped stimulable phosphor layer region 12 among the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 can be irradiated with the laser beam 24. When it is confirmed that the optical head 35 has reached,
A stop signal is output to the main scanning stepping motor 65, and a drive signal is output to the first laser excitation light source 21 to activate the first laser excitation light source 21, and 640 nm
The laser light 24 of the wavelength is emitted.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser beam 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 onto the first dot-shaped stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41. It

As a result, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser beam 24 to generate the first stimulable phosphor. The photostimulable light 45 is emitted from the photostimulable phosphor layer region 12.

The photostimulable light 45 emitted from the first dot-shaped photostimulable phosphor region 12 is condensed by the aspherical lens 37 provided in the optical head 35, and the laser light 24 is reflected by the mirror 36. The light is reflected on the same side as the optical path, becomes parallel light, and enters the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 34 is reflected by the concave mirror 38.
Incident on.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and is incident on the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected by the photomultiplier 50 and generated is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

After the first laser pumping light source 21 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 70 outputs a drive stop signal to the first laser pumping light source 21, The drive of the first laser excitation light source 21 is stopped, and a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move adjacent dots formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor layer regions 12 are moved by a pitch equal to the distance between the regions.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 to form the laser light 24 emitted from the first laser excitation light source 21 on the stimulable phosphor sheet 10. When it is confirmed that the second dot-shaped photostimulable phosphor layer region 12 thus irradiated has been moved to a position where it can be irradiated, the control unit 70 outputs a drive signal to the first laser excitation light source 21. The stimulability contained in the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 by turning on the first laser excitation light source 21 and the laser light 24. Excite the phosphor.

Similarly, the laser light 24 is applied to the second dot-shaped stimulable phosphor layer area 12 formed on the stimulable phosphor sheet 10 for a predetermined time, and the second stimulable phosphor layer 24 is irradiated. The stimulable phosphor contained in the stimulable phosphor layer region 12 is excited, and the stimulable light 45 emitted from the second stimulable phosphor layer region 12 is converted by the photomultiplier 50 into
When photoelectrically detected and analog data is generated,
The control unit 70 includes the first laser excitation light source 2
1 to turn off the first laser excitation light source 21, and the main scanning stepping motor 65
Then, a drive signal is output to set the optical head 35 to a distance equal to the distance between adjacent dot-shaped photostimulable phosphor layer regions 12.
Move only the pitch.

Thus, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal of the optical head 35 input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser light 24 is completed, the control unit 70 Outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to its original position and outputs a drive signal to the sub scanning pulse motor 61 to move the movable substrate 63 to the sub scanning direction. Then, move only one line.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line is moved by one line in the sub-scanning direction, the control unit 70 sequentially applies the first laser excitation to the dot-shaped stimulable phosphor layer region 12 of the first line. Laser light emitted from the first laser excitation light source 21 is sequentially applied to the dot-shaped stimulable phosphor layer region 12 of the second line in the same manner as the irradiation of the laser light 24 emitted from the light source 21. 24 to irradiate 24 to excite the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 to sequentially emit stimulable light 45 emitted from the stimulable phosphor layer region 12. , Photomultiplier 50,
It is detected photoelectrically.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

In this way, the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are all scanned by the laser light 24 emitted from the first laser excitation light source 21, When the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 is excited,
The emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into digital data by the A / D converter 53 and sent to the data processing device 54. A drive stop signal is output from the control unit 70 to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 4 formed in the biochemical analysis unit 1 is read to generate the biochemical analysis digital data, first, the operator The biochemical analysis unit 1 is set on the glass plate 41 of the stage 40.

Next, the operator specifies on the keyboard 71 the type of the fluorescent substance as the labeling substance and inputs an instruction signal to the effect that the fluorescent data should be read.

The instruction signal input to the keyboard 71 is
When the control unit 70 receives the instruction signal, the control unit 70 determines the laser excitation light source to be used according to the table stored in the memory (not shown), and the filter 52.
It is determined which of a, 52b, 52c, and 52d is to be positioned in the optical path of the fluorescent light 45.

[0173] For example, Rhodamine (registered trademark) which can be most efficiently excited by a laser having a wavelength of 532 nm as a fluorescent substance for labeling a substance derived from a living body
Is used and is input to the keyboard 71, the control unit 70 selects the second laser excitation light source 22, selects the filter 52b, and outputs a drive signal to the filter unit motor 72. Then, the filter unit 48 is moved to cut the light having the wavelength of 532 nm, and the filter member 51b having the property of transmitting the light having the wavelength longer than 532 nm is emitted from the biochemical analysis unit 1. It is located in the optical path of the fluorescent light 45 to be formed.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, When it is confirmed that the optical head 35 has reached the position where the laser beam 24 can be irradiated to the first absorptive region 4 among the many absorptive regions 4 formed in the biochemical analysis unit 1. , A stop signal is output to the main scanning stepping motor 65, and a drive signal is output to the second laser excitation light source 22 to activate the second laser excitation light source 22,
A laser beam 24 having a wavelength of 532 nm is emitted.

The laser light 24 emitted from the second laser excitation light source 22 is made into parallel light by the collimator lens 30, and then enters the first dichroic mirror 27 and is reflected.

The laser beam 24 reflected by the first dichroic mirror 27 passes through the second dichroic mirror 28 and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29 and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

Laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 on the biochemical analysis unit 1 mounted on the stage 40 glass plate 41.

As a result, the laser light 24 excites a fluorescent substance such as a fluorescent dye contained in the first adsorptive region 4 of the biochemical analysis unit 1, for example, rhodamine, and emits fluorescence.

Here, in the biochemical analysis unit 1 according to the present embodiment, the absorptive region 4 has the absorptive membrane 2
The aluminum plate 5 having a property of attenuating radiation and light is press-fitted to form an aluminum plate 5 having a property of attenuating the radiation and light around the absorptive region 4. Since the aluminum plate 5 that it has is present, the fluorescent substance contained in the absorptive region 4 is excited,
It is possible to reliably prevent the fluorescence 45 emitted from the fluorescent substance from being mixed with the emitted fluorescence 45 due to the excitation of the fluorescent substance contained in the adsorbing regions 4 adjacent to each other.

The fluorescent light 45 emitted from the rhodamine is collected by the aspherical lens 37 provided in the optical head 35, reflected by the mirror 36 to the same side as the optical path of the laser light 24, and made into parallel light. , Enters the concave mirror 38.

The fluorescent light 45 which has entered the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.

The fluorescence 45 that has entered the perforated mirror 34 is
The perforated mirror 34 formed by the concave mirror reflects the light downward as shown in FIG. 8 and makes it incident on the filter 52b of the filter unit 48.

Since the filter 52b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm which is excitation light is cut and emitted from rhodamine. Fluorescence 45
Only the light in the wavelength range of 1 passes through the filter 52b and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

When a predetermined time, for example, several μ seconds has elapsed after the second laser excitation light source 22 was turned on, the control unit 70 outputs a drive stop signal to the second laser excitation light source 22, The drive of the second laser excitation light source 22 is stopped, and a drive signal is output to the main scanning stepping motor 65 to move the optical head 35 to the distance between the absorptive regions 4 formed in the biochemical analysis unit 1. Move by a pitch equal to.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adjacent absorptive regions 4 formed in the biochemical analysis unit 1 and emitted from the second laser excitation light source 22.
Is confirmed to have moved to a position where the second absorptive region 4 formed in the biochemical analysis unit 1 can be irradiated, the control unit 70 sends a drive signal to the second laser excitation light source 22. Output the second laser excitation light source 2
2 is turned on, and the laser light 24 excites the fluorescent substance contained in the second adsorptive region 4 formed in the biochemical analysis unit 1, for example, rhodamine.

Similarly, the laser beam 24 is irradiated on the second absorptive region 4 formed in the biochemical analysis unit 1 for a predetermined time, and the fluorescence 45 emitted from the second absorptive region 4 is emitted. However, with the photomultiplier 50,
When photoelectrically detected and analog data is generated,
The control unit 70 includes the second laser excitation light source 2
2 to turn off the second laser excitation light source 22 and to output the main scanning stepping motor 65.
Then, a drive signal is output to move the optical head 35 by one pitch equal to the distance between the adsorbing regions 4 formed adjacent to each other in the biochemical analysis unit 1.

In this way, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and when it is confirmed that all the absorptive regions 4 on the first line of the biochemical analysis unit 1 are scanned by the laser beam 24, control is performed. The unit 70 includes the main scanning stepping motor 6
5, a drive signal is output to return the optical head 35 to the original position, and a drive signal is output to the sub-scanning pulse motor 61 to move the movable substrate 63 in the sub-scanning direction.
Move only one line.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the absorptive region 4 of the first line formed in the biochemical analysis unit 1 to be detected.
In the same manner as irradiating the laser beam 24 emitted from the second laser excitation light source 22 in sequence, the absorptive region 4 of the second line formed in the biochemical analysis unit 1
Rhodamine contained in the absorptive region 4 of the second line is excited, and the fluorescence 45 emitted from the absorptive region 4 is sequentially detected photoelectrically by the photomultiplier 50.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processing device 54.

In this way, the entire surface of the biochemical analysis unit 1 is scanned by the laser beam 24 emitted from the second laser excitation light source 22, and a large number of absorptive regions 4 formed in the biochemical analysis unit 1 are formed. Rhodamine contained therein is excited, the emitted fluorescence 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into digital data by the A / D converter 53, When sent to the data processor 54,
The drive stop signal from the control unit 70 is the second
To the laser excitation light source 22 and the driving of the second laser excitation light source 22 is stopped.

FIG. 16 shows the chemiluminescence data of the labeling substance which causes chemiluminescence by contacting with the chemiluminescence substrate recorded in the large number of adsorptive regions 4 formed in the biochemical analysis unit 1, It is a schematic front view of the data generation system which produces | generates the data for biochemical analysis. Figure 15
The data generation system shown in 1 is also configured to be able to generate fluorescence data of a fluorescent substance such as a fluorescent dye recorded in a large number of absorptive regions 4 formed in the biochemical analysis unit 1.

As shown in FIG. 16, the data generation system comprises a cooled CCD camera 81, a dark box 82 and a personal computer 83. The personal computer 83 includes a CRT 84 and a keyboard 85.

FIG. 17 shows the cooling CC of the data generation system.
4 is a schematic vertical sectional view of a D camera 81. FIG.

As shown in FIG. 17, the cooled CCD camera 81 has a CCD 86, a heat transfer plate 87 made of a metal such as aluminum, a Peltier element 88 for cooling the CCD 86, and a front surface of the CCD 86. Shutter 89, A / D converter 90 for converting analog data generated by CCD 86 into digital data, and A / D converter 90
A data buffer 91 for temporarily storing the data digitized by the / D converter 90 and a camera control circuit 92 for controlling the operation of the cooled CCD camera 81 are provided. An opening formed between the dark box 82 and the dark box 82 is closed by a glass plate 95, and a radiation fin 96 for radiating heat generated by the Peltier element 88 is provided around the cooling CCD camera 81 in the longitudinal direction. It is formed over the entire surface.

A camera lens 97 having a lens focus adjusting function is mounted in the dark box 82 in front of the glass plate 95.

FIG. 18 shows a dark box 82 of the data generation system.
FIG.

As shown in FIG. 18, an LED light source 100 that emits excitation light is provided in the dark box 82, and the LED light source 100 is provided with a removable filter 101 and an upper surface of the filter 101. The diffuser plate 103 provided is provided, and the excitation light passes through the diffuser plate 103.
Biochemical analysis unit mounted on it (not shown)
By irradiating the biochemical analysis unit, the biochemical analysis unit is guaranteed to be uniformly irradiated. The filter 101 has a property of cutting light harmful to the excitation of the fluorescent substance other than the wavelength near the excitation light and transmitting only the light of the wavelength near the excitation light. On the front surface of the camera lens 97, a filter 102 that cuts light having a wavelength near the excitation light is provided.
Is detachably provided.

FIG. 19 is a block diagram around the personal computer 83 of the data generating system.

As shown in FIG. 19, the personal computer 83 has a CPU 110 for controlling the exposure of the cooled CCD camera 81, a data transfer means 111 for reading out the digital data generated by the cooled CCD camera 81 from the data buffer 91, and a digital transfer device. On the screen of the CRT 84, based on the data storage means 112 for storing data, the data processing device 113 for performing data processing on the digital data stored in the data storage means 112, and the digital data stored in the data storage means 112. The data display means 114 which displays visible data is provided.

The LED light source 100 has a light source control means 115.
The light source control unit 115 is configured to receive an instruction signal from the keyboard 85 via the CPU 110. The CPU 110 is configured to be able to output various signals to the camera control circuit 92 of the cooled CCD camera 81.

The data generation system shown in FIGS. 16 to 19 is a chemical substance produced by contact between a labeling substance contained in a large number of absorptive regions 4 formed in the biochemical analysis unit 1 and a chemiluminescent substrate. The emitted light is detected by the CCD 86 of the cooled CCD camera 81 through the camera lens 97 to generate chemiluminescence data, and the biochemical analysis unit 1 is irradiated with excitation light from the LED light source 100 to perform biochemical analysis. A fluorescent substance such as a fluorescent dye contained in a large number of absorptive regions 4 formed in the unit 1 is excited and the emitted fluorescence is detected by the CCD 66 of the cooling CCD camera 81 via the camera lens 97. , Fluorescence data can be generated.

When chemiluminescence data is generated, the filter 102 is removed, the LED light source 100 is held in the off state, and the biochemical analysis unit 1 is placed on the diffusion plate 103.
The chemiluminescent substrate is brought into contact with the labeling substance contained in the large number of absorptive regions 4 formed on the biochemical analysis unit 1 that emits chemiluminescence.

Next, the operator focuses the lens using the camera lens 97 and closes the dark box 82.

After that, when the operator inputs an exposure start signal to the keyboard 85, the exposure start signal is sent to the CPU 1
It is input to the camera control circuit 92 of the cooled CCD camera 81 via 10, and the camera control circuit 92 opens the shutter 89 to start the exposure of the CCD 86.

The chemiluminescence emitted from the biochemical analysis unit 1 enters the photoelectric surface of the CCD 86 of the cooled CCD camera 81 through the camera lens 97 and forms an image on the photoelectric surface. The CCD 86 thus receives the light of the image formed on the photocathode and stores it in the form of charges.

Here, in the biochemical analysis unit 1 according to this embodiment, the absorptive region 4 includes the absorptive membrane 2
The aluminum plate 5 having a property of attenuating radiation and light is press-fitted to form an aluminum plate 5 having a property of attenuating the radiation and light around the absorptive region 4. Since the aluminum plate 5 is provided, the chemiluminescence emitted from the labeling substance contained in the adsorptive region 4 is mixed with the chemiluminescence emitted from the labeling substance contained in the adjacent adsorptive region 4. It can be surely prevented from fitting.

When the predetermined exposure time elapses, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 110, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charge to the A / D converter 100, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 11 to operate.
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When the operator inputs a data display signal to the keyboard 85, the CPU 110 causes the data storage means 1 to operate.
The digital data stored in 12 is output to the data processing device 113, data processing is performed in accordance with the instruction of the operator, and then a data display signal is output to the data display means 114 to generate chemiluminescence based on the digital data. The data is displayed on the screen of the CRT 84.

On the other hand, when generating fluorescence data, first, the biochemical analysis unit 1 is placed on the diffusion plate 103.

Next, the LED light source 1 is operated by the operator.
00 is turned on, lens focusing is performed using the camera lens 97, and the dark box 82 is closed.

Then, when the operator inputs an exposure start signal to the keyboard 85, the light source control means 115 turns on the LED light source 100, and the excitation light is emitted toward the biochemical analysis unit 1. At the same time, the exposure start signal is sent to the cooling CCD camera 8 via the CPU 110.
1 to the camera control circuit 92, and the camera control circuit 9
2, the shutter 89 is opened and the exposure of the CCD 86 is started.

The excitation light emitted from the LED light source 100 is filtered by the filter 101 to eliminate wavelength components other than the excitation light, and is made uniform by the diffusion plate 23, and is applied to the biochemical analysis unit 1. It

When the biochemical analysis unit 1 is irradiated with the excitation light, the fluorescent substances contained in the many absorptive regions 4 formed in the biochemical analysis unit 1 are excited by the excitation light. , Fluorescence is emitted.

The fluorescence emitted from the biochemical analysis unit 1 enters the photocathode of the CCD 86 of the cooled CCD camera 81 through the filter 102 and the camera lens 97 and forms an image on the photocathode. CCD86 is
It receives the light of the image formed on the photocathode and stores it in the form of charges. Since the filter 102 cuts off the light of the wavelength of the excitation light, only the fluorescence emitted from the fluorescent substance contained in the many absorptive regions 4 formed in the biochemical analysis unit 1 is received by the CCD 86. To be done.

Here, in the biochemical analysis unit 1 according to this embodiment, the absorptive region 4 is the absorptive membrane 2
The aluminum plate 5 having a property of attenuating radiation and light is press-fitted to form an aluminum plate 5 having a property of attenuating the radiation and light around the absorptive region 4. The presence of the aluminum plate 5 has, so that the fluorescence emitted from the fluorescent substance contained in the absorptive region 4 is mixed with the fluorescence emitted from the fluorescent substance contained in the adjacent absorptive region 4. Can be reliably prevented.

When the predetermined exposure time elapses, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 40, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charge to the A / D converter 10, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 21
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When an operator inputs a data display signal to the keyboard 85, the CPU 110 causes the data storage means 1 to operate.
The digital data stored in 12 is output to the data processing device 113, the data processing is performed according to the instruction of the operator, and then the data display signal is output to the data display means 114, and the fluorescence data is output based on the digital data. Is displayed on the screen of the CRT 84.

According to this embodiment, the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 is formed on the support 11 of the stimulable phosphor sheet 10. Dot-shaped photostimulable phosphor layer region 12
During exposure, a large number of absorptive regions 4 of the biochemical analysis unit 1 have a property of attenuating radiation into the absorptive film 2, and an aluminum plate 5 having a large number of through holes 4 formed therein is press-fitted. Around the absorptive region 4, there is an aluminum plate 5 having a property of attenuating radiation,
Further, since the support 11 of the stimulable phosphor sheet 10 is made of stainless steel having a property of attenuating radiation, the electron beam emitted from the radiolabel substance contained in the absorptive region 4 is scattered. The electron beam emitted from the radio-labeled substance contained in the adsorptive region 4 can be completely prevented.
Incident on the photostimulable phosphor layer region 12 opposed to, and therefore, even if the absorptive regions 4 are formed in the biochemical analysis unit 1 at a high density, the electrons emitted from the adjacent absorptive regions 4 are emitted. Photostimulable phosphor layer region 12 to be exposed by rays
Since it is possible to reliably prevent light from entering the device, noise caused by electron beam scattering may be generated in the biochemical analysis data generated by photoelectrically detecting the stimulated emission 45. Can be effectively prevented and the quantitativeness of biochemical analysis can be improved.

Further, according to this embodiment, a large number of the absorptive regions 4 of the biochemical analysis unit 1 have a property of attenuating radiation and light in the absorptive film 2, and have a large number of through-holes 4.
The aluminum plate 5 on which the biochemical analysis unit 1 is formed is formed by being press-fitted and formed, and the aluminum plate 5 having a property of attenuating radiation and light is present around each absorptive region 4. The fluorescence emitted from the fluorescent substance contained in the many absorptive regions 4 formed in
It is possible to reliably prevent the fluorescent substances contained in the adsorbing regions 4 adjacent to each other from being excited and being mixed with the emitted fluorescence, and therefore, the large number of adsorbing substances formed in the biochemical analysis unit 1 can be prevented. The fluorescent substance contained in the region 4 is irradiated with excitation light to excite the fluorescent substance, and the fluorescence emitted from the fluorescent substance is photoelectrically detected to generate biochemical analysis data. It is possible to reliably prevent the generation of noise due to the scattering of the, and improve the quantitativeness of the biochemical analysis.

Further, according to this embodiment, the absorptive region 4 of the biochemical analysis unit 1 has a property of attenuating radiation and light in the absorptive film 2, and a large number of through holes 4 are formed. Since the aluminum plate 5 is formed by being press-fitted and the absorptive region 4 has the aluminum plate 5 having a property of attenuating radiation and light, it is formed in the biochemical analysis unit 1. It is possible to reliably prevent the chemiluminescence emitted from the large number of adsorptive regions 4 from being mixed with the chemiluminescence emitted from the adjacent adsorptive regions 4, and thus the chemiluminescence emitted from the biochemical analysis unit 1 is formed. In addition, it is possible to reliably prevent generation of noise due to chemiluminescence scattering in the biochemical analysis data generated by photoelectrically detecting chemiluminescence emitted from the large number of adsorptive regions 4. , Birth It is possible to improve the quantification of the analysis.

Further, according to this embodiment, the absorptive region 4 of the biochemical analysis unit 1 is provided on the absorptive membrane 2 formed of nylon 6 capable of forming a membrane filter,
Aluminum plate 5 in which a large number of through holes 4 are regularly formed
Since the holes are formed in the adsorptive film 2 located between the adjacent adsorptive regions 4 by press-fitting, the holes are eliminated by pressurization when the aluminum plate 5 is press-fitted. The specific binding substance dropped in the adsorptive region 4 is
It is effectively prevented from diffusing into the region of the adsorptive film 2 other than the adsorptive region 4, and the specific binding substance dropped in the adsorptive region 4 is adsorbed only in the adsorptive region 4, It becomes possible to greatly improve the quantitativeness of chemical analysis.

Furthermore, according to this embodiment, the biochemical analysis unit 1 is formed by press-fitting the aluminum plate 5 into the adsorptive membrane 2 formed of nylon 6 capable of forming a membrane filter. Therefore, even if it is treated with a liquid such as hybridization, it hardly expands or contracts, and the large number of absorptive regions 4 formed in the biochemical analysis unit 1 are attached to the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are easily and surely overlapped with each other so as to accurately face the many dot-shaped stimulable phosphor layer regions 12 formed, It becomes possible to expose the dot-shaped stimulable phosphor layer region 12.

FIG. 20 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.
1 is a partial sectional view thereof.

As shown in FIGS. 20 and 21, the biochemical analysis unit 121 according to this embodiment is similar to that shown in FIG.
And an adsorptive membrane 122 made of nylon 6 capable of forming a membrane filter, similar to the embodiment shown in FIG. 2, in which the metal oxide particles are dispersed in plastic. Then, the plastic substrate 125 in which the substantially circular through holes 124 are regularly formed is press-fitted, whereby a large number of suction holes are formed corresponding to the large number of through holes 124 formed in the plastic substrate 125. The characteristic regions 124 are regularly formed in a dot shape. Here, metal oxide particles are dispersed in plastic to form a plastic substrate 125.
Has a property of attenuating radiation and light. An adhesive 123 is applied to the back surface of the plastic substrate 125, and the plastic substrate 125 is bonded via the adhesive 123.
Are pressed into the adsorptive film 122. Therefore, the plastic substrate 125 is firmly integrated with the adsorptive film 122, and the durability is improved.

Although not shown exactly in FIG. 20, in the present embodiment, the generally circular absorptive region 124 has a size of about 0.01 square millimeters of about 10,000.
Are regularly formed in the biochemical analysis unit 121 at a density of about 5000 pieces / square centimeter.

As shown in FIG. 21, in this embodiment, the plastic substrate 125 is disposed on the adsorptive film 122 so that the surface of the plastic substrate 125 is located above the surface of each of the adsorptive regions 124. The biochemical analysis unit 121 is formed by press-fitting.

In the present embodiment, the biochemical analysis unit 121 is formed by press-fitting the absorptive film 122 into the through holes 124 formed in the plastic substrate 125 using a hot press processing apparatus. .

FIG. 22 is a schematic sectional view of the hot press processing apparatus.

As shown in FIG. 22, the hot press processing apparatus has a base 127 and a temperature-controlled press plate 128.

First, the absorptive 122 is set on the base 127, and the plastic substrate 125 having a large number of through holes 124 is set on the absorptive film 122.

Next, the plastic substrate 125 is pressed by the press plate 128, and the plastic substrate 125 is pressed.
The absorptive film 122 is press-fitted into the through-holes 124 formed in the above to form a large number of absorptive regions 124.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
Similarly to the above, a large number of absorptive regions 12 formed in the biochemical analysis unit 121 by a spotting device.
A specific binding substance such as cDNA is dropped onto 4 and is adsorbed.

Also in this embodiment, the absorptive region 124 of the biochemical analysis unit 121 is formed in the absorptive film 122,
Since the plastic substrate 125 in which a large number of through holes 124 are regularly formed is press-fitted and formed, the holes in the adsorptive film 122 located between the adjacent adsorptive regions 124 disappear by the pressurization. Therefore, the specific binding substance dropped in the absorptive region 124 is effectively prevented from penetrating into the region of the absorptive film 122 other than the absorptive region 124. The dropped specific binding substance is adsorbed only to the adsorptive region 124.

Further, as shown in FIG. 5, chemiluminescence is obtained by contacting a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. Hybridization container 8 containing a hybridization liquid 9 containing a substance of biological origin labeled with a labeling substance that causes
A biochemical analysis unit 121 is set therein, and a specific binding substance such as cDNA adsorbed on a large number of absorptive regions 124 is labeled with a radioactive labeling substance, and is derived from a living body contained in the hybridizing solution 9. A substance, a fluorescent substance such as a fluorescent dye, and a biogenic substance contained in the hybridizing liquid 9 and a biogenic substance contained in the hybridizing liquid 9 and labeled with a labeling substance that causes chemiluminescence. Selectively hybridize.

Thus, the biochemical analysis unit 121
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

Fluorescence data recorded in the biochemical analysis unit 121 is recorded in the scanner shown in FIGS. 8 to 15 or FIGS.
The cooled CCD camera 81 of the data generation system shown in FIG.
By the above, the biochemical analysis data is read and the chemiluminescence data recorded in the biochemical analysis unit 121 is the data shown in FIGS. 16 to 19 in the same manner as in the above embodiment. Cooling CCD for production system
The data is read by the camera 81 and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 12
The radiation data of the radiolabeled substance recorded in 1 is transferred to the stimulable phosphor sheet.

FIG. 23 is a schematic perspective view of the stimulable phosphor sheet 130.

As shown in FIG. 23, the stimulable phosphor sheet 130 includes a support 131, and one surface of the support 131 has a large number of absorptive regions formed in the biochemical analysis unit 121. A large number of substantially circular dot-shaped stimulable phosphor layer regions 132 are formed in the same regular pattern as the pattern 124.

Also in this embodiment, the support 131 is
A stimulable phosphor layer region 132 formed of stainless steel having a property of attenuating radiation and having a substantially circular dot shape.
Are formed on the surface of the support 121 so as to have the same diameter as that of the absorptive region 124 formed in the biochemical analysis unit 121.

FIG. 24 shows a large number of dot-shaped stimulable fluorescent substances formed on the stimulable phosphor sheet 130 by the radioactive labeling substance contained in the large number of absorptive regions 124 formed on the biochemical analysis unit 121. FIG. 6 is a schematic cross-sectional view showing a method of exposing the body layer region 132.

As shown in FIG. 24, upon exposure, a large number of absorptive regions 124 formed in the biochemical analysis unit 121 are formed on the surface of the support 131 of the stimulable phosphor sheet 130. The stimulable phosphor sheet 130 and the biochemical analysis unit 121 are superposed so as to face the dot-shaped stimulable phosphor layer region 132.

Also in this embodiment, the biochemical analysis unit 121 has a plastic substrate 12 in which metal oxide particles are dispersed in a plastic on an adsorptive film 122 formed of nylon 6 capable of forming a membrane filter.
Since 5 is press-fitted and formed, it hardly expands or contracts even when subjected to a treatment with a liquid such as hybridization. Therefore, a large number of absorptive regions 124 formed in the biochemical analysis unit 121 are formed. , The biochemical analysis with the stimulable phosphor sheet 130 so that the dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of the stimulable phosphor sheet 130 are accurately opposed to each other. The dot-shaped stimulable phosphor layer region 132 can be exposed by easily and surely overlapping the unit 121 for use.

In this way, each of a large number of dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of the stimulable phosphor sheet 130 and the biochemical analysis unit 121 over a predetermined period of time. The large number of dots formed on the surface of the support 131 of the stimulable phosphor sheet 130 by the radioactive labeling substance contained in the absorptive region 124 by facing the large number of absorptive regions 124 formed in the. The photostimulable phosphor layer region 132 is exposed.

At this time, an electron beam is emitted from the radio-labeled substance adsorbed in the absorptive region 124, but the absorptive region 124 of the biochemical analysis unit 121 is absorptive film 1
A large number of through holes 124 are formed in 22 and a plastic substrate 125 having a property of attenuating the radiation is press-fitted to be formed. A plastic substrate 125 having a property of attenuating the radiation is formed around the absorptive region 124. Since it is present, the electron beam emitted from the radio-labeled substance contained in the absorptive region 124 is surely prevented from scattering, and emitted from the radio-labeled substance contained in each absorptive region 124. All electron beams are incident on the photostimulable phosphor layer region 132 facing the adsorptive region 124,
It is possible to reliably prevent the electron beam emitted from the adjacent adsorptive region 124 from entering the stimulable phosphor layer region 132 to be exposed.

Therefore, a large number of dot-shaped stimulable phosphor layer regions 132 formed on the stimulable phosphor sheet 130.
Can be reliably exposed only by the radioactive labeling substance contained in the corresponding adsorptive region 124 of the biochemical analysis unit 121.

Thus, a large number of dot-shaped stimulable phosphor layer regions 132 formed on the stimulable phosphor sheet 130 are
The radiation data of the radiolabeled substance is recorded, and the radiation data recorded in a large number of dot-shaped stimulable phosphor layer regions 132 is the same as in the above-described embodiment, and the scanner shown in FIGS. Is read out and biochemical analysis data is generated.

According to this embodiment, the stimulable phosphor sheet 1 is made by the radioactive labeling substance contained in the many absorptive regions 124 formed in the biochemical analysis unit 121.
When exposing the dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of 30, the many absorptive regions 124 of the biochemical analysis unit 121 are treated with the absorptive film 1
A plastic substrate 125 having a property of attenuating radiation and having a large number of through holes 4 formed therein is press-fitted to 22.
A plastic substrate 125 having a property of attenuating radiation exists around the formed absorptive region 124,
Furthermore, the support 131 of the stimulable phosphor sheet 130 is
Since it is formed of stainless steel having a property of attenuating radiation, it is possible to reliably prevent the electron beam emitted from the radiolabeled substance contained in the absorptive region 124 from scattering, and the absorptive region 124 can be reliably prevented. All the electron beams emitted from the radiolabeled substance contained in the stimulable phosphor layer region 13 facing the adsorptive region 124.
2 and therefore the biochemical analysis unit 121
In addition, even if the absorptive regions 124 are formed at a high density, the electron beams emitted from the adjacent absorptive regions 124 are surely prevented from entering the stimulable phosphor layer region 132 to be exposed. Therefore, it is possible to effectively prevent the generation of noise due to the scattering of electron beams in the biochemical analysis data generated by photoelectrically detecting stimulated emission and perform biochemical analysis. It is possible to improve the quantitativeness of

Further, according to this embodiment, the large number of absorptive regions 124 of the biochemical analysis unit 121 has a property of attenuating radiation and light in the absorptive film 122, and a large number of through holes 124 are formed. Formed plastic substrate 125
Since a plastic substrate 125 having a property of attenuating radiation and light is present around each absorptive region 124 formed by being pressed in, a large number of absorptive substances formed in the biochemical analysis unit 121 are adsorbed. It is possible to reliably prevent the fluorescence emitted from the fluorescent substance contained in the fluorescent region 124 from being mixed with the emitted fluorescent substance due to the excitation of the fluorescent substance contained in the adjacent adsorptive region 124. Therefore, the fluorescent substance contained in the many absorptive regions 124 formed in the biochemical analysis unit 121 is irradiated with the excitation light to excite the fluorescent substance, and the fluorescence emitted from the fluorescent substance is photoelectrically converted. It is possible to reliably prevent the generation of noise due to the scattering of fluorescence in the data for biochemical analysis that is detected and generated, and improve the quantitativeness of biochemical analysis. That.

Further, according to this embodiment, the large number of absorptive regions 124 of the biochemical analysis unit 121 have a property of attenuating radiation and light in the absorptive film 122,
Plastic substrate 12 having a large number of through holes 124 formed therein
5 is press-fitted and formed, and a plastic substrate 125 having a property of attenuating radiation and light is present around each absorptive region 124. Therefore, a large number of plastic substrates 125 formed in the biochemical analysis unit 121 are formed. It is possible to reliably prevent the chemiluminescence emitted from the absorptive region 124 from mixing with the chemiluminescence emitted from the adjacent absorptive region 124, and thus the biochemical analysis unit 121.
It is ensured that noise resulting from chemiluminescence scattering is generated in the biochemical analysis data generated by photoelectrically detecting chemiluminescence emitted from the large number of absorptive regions 124 formed in It is possible to prevent and improve the quantitativeness of biochemical analysis.

Further, according to the present embodiment, the plurality of absorptive regions 124 of the biochemical analysis unit 121 are provided with a plurality of through holes 124 in the absorptive film 122 formed of nylon 6 capable of forming a membrane filter. Are formed by press-fitting the plastic substrate 125 that is regularly formed, the holes in the adsorbent film 122 located between the adsorbing regions 124 adjacent to each other form holes when the plastic substrate 125 is press-fitted. The specific binding substance dropped in the adsorptive region 124 is effectively prevented from diffusing into the region of the adsorptive film 122 other than the adsorptive region 124 because it has disappeared due to the pressurization. Since the specific binding substance dropped in the region 124 is adsorbed only to the adsorptive region 124, it becomes possible to greatly improve the quantitativeness of the biochemical analysis.

Further, according to this embodiment, the biochemical analysis unit 121 is formed by press-fitting the plastic substrate 125 into the adsorptive film 122 formed of nylon 6 capable of forming a membrane filter. Therefore, even if it is subjected to a treatment with a liquid such as hybridization, it hardly expands or contracts, and a large number of the absorptive regions 124 formed in the biochemical analysis unit 1 are formed in the support 131 of the stimulable phosphor sheet 130. The stimulable phosphor sheet 130 and the biochemical analysis unit 121 are easily and surely overlapped with each other so as to accurately face the large number of dot-shaped stimulable phosphor layer regions 132 formed on the surface. In addition, the dot-shaped stimulable phosphor layer region 132 can be exposed.

FIG. 25 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.
6 is a schematic partial sectional view thereof.

As shown in FIGS. 25 and 26, the biochemical analysis unit 141 according to this embodiment includes an adsorbent film 142 made of nylon 6 capable of forming a membrane filter. In this area, regular dot-shaped absorptive regions 144 are formed.

In the present embodiment, the dot-shaped absorptive regions 144 are absorptive of a dispersion liquid containing metal colloid simple particles having a property of attenuating radiation and light, leaving regular dot-shaped regions. Let the membrane 142 soak in,
Shielding area 14 having the property of attenuating radiation and light
It is formed by forming 5.

The dot-shaped absorptive region 144 has, for example, the same pattern as that of the dot-shaped absorptive region 144 to be formed, and uses a plate-shaped member having a large number of legs formed thereon. Of the absorptive film 142, the region where the dot-shaped absorptive region 144 of the absorptive film 142 is to be formed is masked by the large number of legs, and the absorptive film not masked by the large number of legs. It can be formed by impregnating 142 with a dispersion liquid containing colloidal metal particles having a property of attenuating radiation and light.

Although not shown exactly in FIG. 25, in the present embodiment a generally circular adsorptive region 144 having a size of about 0.01 square millimeters of about 10,000 is provided.
Are regularly formed in the biochemical analysis unit 141 at a density of about 5000 pieces / square centimeter.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
In the same manner as described above, a large number of absorptive regions 14 formed in the biochemical analysis unit 141 by the spotting device
A specific binding substance such as cDNA is dropped onto 4 and is adsorbed.

Furthermore, as shown in FIG. 5, chemiluminescence is obtained by contacting a biogenic substance labeled with a radioactive labeling substance, a biogenic substance labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. Hybridization container 8 containing a hybridization liquid 9 containing a substance of biological origin labeled with a labeling substance that causes
A biochemical analysis unit 141 is set therein, and a specific binding substance such as cDNA adsorbed on a large number of absorptive regions 144 is labeled with a radioactive labeling substance, A substance, a fluorescent substance such as a fluorescent dye, and a biogenic substance contained in the hybridizing liquid 9 and a biogenic substance contained in the hybridizing liquid 9 and labeled with a labeling substance that causes chemiluminescence. Selectively hybridize.

Thus, the biochemical analysis unit 141
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

The fluorescence data recorded in the biochemical analysis unit 141 is the same as in the above embodiment, and the scanner shown in FIGS. 8 to 15 or FIGS.
The cooled CCD camera 81 of the data generation system shown in FIG.
By the above, the biochemical analysis data is read, and the chemiluminescence data recorded in the biochemical analysis unit 141 is the data shown in FIGS. 16 to 19 in the same manner as in the above embodiment. Cooling CCD for production system
The data is read by the camera 81 and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 14
The radiation data of the radiolabeled substance recorded in 1 is shown in Figure 1.
And the biochemical analysis unit 1 shown in FIG. 2 and transferred to the stimulable phosphor sheet 10 shown in FIG. 6 in exactly the same manner as in the above embodiment. The shown scanner will read and generate data for biochemical analysis.

According to this embodiment, the stimulable phosphor sheet 1 is prepared by the radioactive labeling substance contained in the many absorptive regions 144 formed in the biochemical analysis unit 141.
When the dot-shaped photostimulable phosphor layer region 12 formed on the support 11 of No. 0 is exposed, the large number of absorptive regions 144 of the biochemical analysis unit 141 are made of metal having a property of attenuating radiation and light. A dispersion containing simple colloid particles,
It is formed by allowing absorptive film 142 to soak into a regular dot-like region and forming a shielding region 145 having a property of attenuating radiation and light, and surrounding each absorptive region 144. Has a shielding region 145 having a property of attenuating radiation and light, and further, since the support 11 of the stimulable phosphor sheet 10 is formed of stainless steel having a property of attenuating radiation, it has an adsorptive property. The electron beam emitted from the radiolabeled substance contained in the region 144 can be reliably prevented from scattering, and all the electron beams emitted from the radiolabeled substance contained in the adsorptive region 144 are It is incident on the photostimulable phosphor layer region 12 facing the absorptive region 144, and therefore, the absorptive region 1 is densely applied to the biochemical analysis unit 141. 4 can be surely prevented from entering the stimulable phosphor layer region 12 to be exposed by the electron beam emitted from the adjacent absorptive region 144. It is possible to effectively prevent the generation of noise due to the scattering of electron beams in the data for biochemical analysis generated by photoelectrically detecting light and improve the quantitativeness of biochemical analysis. become.

Further, according to the present embodiment, a large number of the absorptive regions 144 of the biochemical analysis unit 141 are made into a regular dot with a dispersion liquid containing colloidal metal particles having a property of attenuating radiation and light. -Shaped regions are left to soak the adsorptive film 142 to form the shield regions 145 having the property of attenuating the radiation and the light, and the radiation and the light are formed around each of the adsorptive regions 144. Since there is the shielding region 145 having the property of attenuating, the fluorescence emitted from the fluorescent substance contained in the many absorptive regions 144 formed in the biochemical analysis unit 141 is adjacent to the absorptive substances. It is possible to reliably prevent the fluorescent substance contained in the region 144 from being excited and mixed with the emitted fluorescent light, and therefore, the biochemical analysis unit. The number of the fluorescent substance contained in the absorptive regions 144 formed in 41 is irradiated with excitation light,
Exciting the fluorescent substance, in the data for biochemical analysis generated by photoelectrically detecting the fluorescence emitted from the fluorescent substance, it is possible to reliably prevent the generation of noise due to the scattering of the fluorescence, It becomes possible to improve the quantitativeness of biochemical analysis.

Furthermore, according to the present embodiment, a large number of absorptive regions 144 of the biochemical analysis unit 141 are formed by regularly dispersing the dispersion liquid containing colloidal metal particles having the property of attenuating radiation and light. Leaves the area
It is formed by impregnating the absorptive film 142 and forming a shield region 145 having a property of attenuating radiation and light, and a shield having a property of attenuating the radiation and light is formed around each of the absorptive regions 144. Since the region 145 exists, the chemiluminescence emitted from each absorptive region 124 formed in the biochemical analysis unit 121 is
It is possible to reliably prevent the chemiluminescence emitted from the adjacent absorptive regions 124 from being mixed with each other, and therefore, the chemiluminescence emitted from the large number of absorptive regions 124 formed in the biochemical analysis unit 121 can be prevented. It is possible to reliably prevent the generation of noise due to chemiluminescence scattering in the data for biochemical analysis generated by photoelectric detection, and improve the quantitativeness of biochemical analysis. Become. The present invention is not limited to the above embodiments,
It goes without saying that various modifications are possible within the scope of the invention described in the claims and they are also included within the scope of the present invention.

For example, in the above-mentioned embodiment, a plurality of cDNAs having different known base sequences are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to the cDNA. Hormones, tumor markers, enzymes, antibodies, antigens,
Abzyme, other proteins, nucleic acids, cDNA, D
All specific binding substances, such as NA and RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. are known, should be used as the specific binding substance of the present invention. You can

Further, in the above-mentioned embodiment, the adsorptive membranes 2, 12 of the biochemical analysis units 1, 121, 141 are used.
Both 2 and 142 are made of nylon 6 capable of forming a membrane filter, but the absorptive membranes 2, 122, 1 of the biochemical analysis units 1, 121, 141
It is not always necessary that 42 is made of nylon 6. Instead of nylon 6, a carbon porous material such as activated carbon or a porous material capable of forming a membrane filter is used, and biochemical analysis units 1, 12 are formed.
It is also possible to form the absorptive films 2, 122, 142 of 1, 141. As the porous material capable of forming the membrane filter, for example, nylon 6, nylon 6,6,
Nylons such as nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid such as alginic acid / polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polychlorination Vinyl; polyvinylidene chloride; polyvinylidene fluoride, polyfluorides such as polytetrafluoride, and copolymers or composites thereof. Furthermore, platinum, gold,
Metals such as iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite;
The biochemical analysis unit 1, 121, 1 is prepared by using an inorganic porous material such as a metal salt of hydroxyapatite or calcium sulfate or a complex thereof, or a bundle of a plurality of fibers.
It is also possible to form the 41 absorptive membranes 2, 122, 142.

Further, in the embodiment shown in FIGS. 1 and 2, the biochemical analysis unit 1 is produced by press-fitting the aluminum plate 5 into the adsorptive membrane 2 using a calender roll. In the embodiment shown in FIGS. 20 and 21, the biochemical analysis unit 121 is a plastic obtained by dispersing metal oxide particles in plastic on the adsorptive film 122 using a hot press treatment device. Although the substrate 125 is press-fitted and generated, the aluminum plate 5 is formed by using a calender roll or a heat press processing device.
Alternatively, the plastic substrate 125 may be attached to the adsorbent films 2, 12
It is not always necessary to press fit into the aluminum plate 2, and the aluminum plate 5 or the plastic substrate 12 may be formed by using other means.
5 may be press-fitted into the absorptive films 2 and 122, or instead of the press-fitting, the aluminum plate 5 or the plastic substrate 125 may be attached to the absorptive films 2 and 122 by an appropriate method.
Embedded in 122, biochemical analysis unit 1, 121
May be formed.

In the embodiment shown in FIGS. 1 and 2, the aluminum plate 5 is press-fitted into the adsorptive membrane 2 to produce the biochemical analysis unit 1, which is shown in FIGS. 20 and 21. In the disclosed embodiment, the adsorptive membrane 122
A plastic substrate 125 obtained by dispersing metal oxide particles in plastic is press-fitted into the biochemical analysis unit 121, and the metal oxide particles are dispersed in aluminum 5 or plastic. It is not always necessary to use the obtained plastic substrate 125,
It is formed of a material having a property of attenuating radiation and light and is not particularly limited as long as it is a plate-shaped member having a plurality of through holes formed therein, an inorganic compound material,
A plate-shaped member formed of any organic compound material can be used, and a plate-shaped member formed of a metal material, a ceramic material or a plastic material is preferably used. Examples of the inorganic compound material that can be preferably used to form the plate-shaped member and can attenuate radiation and / or light include gold, silver, and
Copper, zinc, aluminum, titanium, tantalum, chrome,
Metals such as iron, nickel, cobalt, lead, tin and selenium;
Alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; tungsten carbide, calcium carbonate, sulfuric acid. Inorganic salts such as calcium, hydroxyapatite and gallium arsenide can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, as the organic compound material capable of attenuating radiation and / or light, a polymer compound is preferably used, and the radiation and / or light preferably usable for forming the plate-shaped member can be attenuated. Examples of the polymer compound include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoro. Ethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon such as Nylon 4, 10; Poly Bromide; polysulfone;
Polyphenylene sulfide; Silicon resin such as polydiphenyl siloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose Examples include sugars, chitin, chitosan, sumac, polyamide such as gelatin, collagen, keratin, and copolymers of these polymer compounds. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

In the embodiment shown in FIGS. 1 and 2, the aluminum plate 5 is press-fitted into the adsorptive film 2 via the adhesive 3 to form the biochemical analysis unit 1. In the embodiment shown in FIGS. 20 and 21, a plastic substrate 125 produced by dispersing metal oxide particles in plastic is press-fitted into the adsorptive film 122 via an adhesive 123 for biochemical analysis. Unit 121
However, it is not always necessary to use the adhesives 3, 123.

Further, in the embodiment shown in FIGS. 1 and 2, the aluminum plate 5 is press-fitted into the absorptive film 2 so that the radiation and the light are attenuated around the absorptive regions 4. The biochemical analysis unit 1 is produced such that the aluminum plate 5 that it has is present, and in the embodiment shown in FIGS. 20 and 21, the adsorptive membrane 122 is provided.
A plastic substrate 125 formed by dispersing metal oxide particles in plastic is press-fitted into the
The biochemical analysis unit 121 is generated so that a plastic substrate 125 having a property of attenuating radiation and light is present around 24, and further, in the embodiment shown in FIGS. 25 and 26, The biochemical analysis unit 141 is generated so that a shielding region 145 having a property of attenuating radiation and light is formed around each absorptive region 144. In any of the embodiments, each absorptive region is formed. Although a region having a property of attenuating radiation and light is formed around the regions 4, 124, 144, a large number of dot-shaped stimulable phosphor layer regions 12 of the stimulable phosphor sheets 10, 130 are formed. , 132 to detect only the radiation data recorded, the biochemical analysis data is generated, the absorptive regions 4, 124, 14
4, a region having a property of transmitting radiation but attenuating radiation may be formed around 4, while a large number of absorptive regions 4 of the biochemical analysis units 1, 121, 141 are formed. When only the fluorescence data or the chemiluminescence data recorded in 124, 144 is detected to generate the biochemical analysis data, the absorptive regions 4, 124,
A region having a property of transmitting light but attenuating light may be formed around 144.

Further, in the embodiment shown in FIGS. 1 and 2, the embodiment shown in FIGS. 20 and 21 and the embodiments shown in FIGS. 25 and 26, both are about 10,000. Substantially circular adsorptive regions 4,124,144 having a size of 0.01 mm2
Are regularly formed in the biochemical analysis units 1, 121, 141 at a density of about 5000 pieces / square centimeter, but the absorptive regions 4, 124, 144 are not necessarily formed in a substantially circular shape. Not necessarily, the absorptive regions 4, 124, 144 can be formed in any shape, for example rectangular.

Further, in the embodiment shown in FIGS. 1 and 2, the embodiment shown in FIGS. 20 and 21 and the embodiments shown in FIGS. 25 and 26, both are about 10,000. Substantially circular adsorptive regions 4,124,144 having a size of 0.01 mm2
Are regularly formed on the biochemical analysis units 1, 121, 141 at a density of about 5000 pieces / square centimeter, and the number and size of the absorptive regions 4, 124, 144 are determined according to the purpose. , Adsorbent regions 4, 124, 144 having a size of 10 or more and preferably less than 5 mm <2>.
Are formed in the biochemical analysis units 1, 121, 141 at a density of 10 or more per square centimeter.

Furthermore, in the embodiment shown in FIGS. 1 and 2, the embodiment shown in FIGS. 20 and 21 and the embodiments shown in FIGS. 25 and 26, both are about 10,000. Substantially circular adsorptive regions 4,124,144 having a size of 0.01 mm2
Are regularly formed in the biochemical analysis unit 1, 121, 141 at a density of about 5000 pieces / square centimeter, and the absorptive regions 4, 124, 144 are regularly formed for biochemical analysis. It is not always necessary to form the units 1, 121 and 141.

Further, in the above-mentioned embodiment, chemiluminescence is generated by contacting with a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. A hybridizing liquid 9 containing a substance derived from a living body labeled with the labeling substance to be prepared is prepared and hybridized with the specific binding substance dropped on the adsorptive material 4.
It is not always necessary that the biological substance is labeled with a radioactive labeling substance, a fluorescent substance such as a fluorescent dye, and a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate. In addition to the substance, it may be labeled with at least one type of labeling substance that causes chemiluminescence by contacting with a fluorescent substance and a chemiluminescent substrate.

Furthermore, in the above-mentioned embodiment, a substance derived from a living body which is labeled with a radioactive labeling substance, a fluorescent substance such as a fluorescent dye, and a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate is specifically bound. Although it is hybridized to the substance,
It is not always necessary to hybridize to a specific binding substance, and a substance of biological origin is specifically bound to a specific binding substance by an antigen-antibody reaction, a reaction such as a receptor / ligand, etc., instead of hybridization. You can also let it.

Further, in the above embodiment, the absorptive region 4 formed in the biochemical analysis unit 1, 121,
A substantially circular stimulable phosphor layer region 12, which has the same regular pattern as 124 and has the same size as the absorptive regions 4 and 124 formed in the biochemical analysis units 1 and 121;
132 is a support 1 for the stimulable phosphor sheets 10 and 130.
1 and 131, the stimulable phosphor layer region 1
It is not always necessary that 2, 132 have the same size as the absorptive regions 4, 124 formed in the biochemical analysis unit 1, 121. Preferably, the biochemical analysis unit 1, 121 has the same size. Formed absorptive regions 4, 12
It is formed to a size of 4 or more.

In the above embodiment, the absorptive regions 4 and 1 formed in the biochemical analysis unit 1 and 121.
24, a substantially circular stimulable phosphor layer region 12, 1 having the same regular pattern as 24 and having the same size as the absorptive region 4, 124 formed in the biochemical analysis unit 1, 121.
32 is a support 1 for the stimulable phosphor sheets 10 and 130.
1 and 131, the stimulable phosphor layer region 1
It is sufficient that 2, 132 are formed in the same pattern as the absorptive regions 4, 124 formed in the biochemical analysis unit 1, 121, and do not necessarily need to be formed regularly.

Further, in the above embodiment, the absorptive region 4 formed in the biochemical analysis unit 1, 121,
A substantially circular stimulable phosphor layer region 12, which has the same regular pattern as 124 and has the same size as the absorptive regions 4 and 124 formed in the biochemical analysis units 1 and 121;
132 is a support 1 for the stimulable phosphor sheets 10 and 130.
1 and 131, the stimulable phosphor layer region 1
It is not always necessary to form 2, 132 in a substantially circular shape, and it may be formed in an arbitrary shape, for example, a rectangular shape.

Further, in the above-mentioned embodiment, the supports 11, 131 of the stimulable phosphor sheets 10, 130 are made of stainless steel, but the supports 11, 131 of the stimulable phosphor sheets 10, 130 are not. It does not necessarily have to be formed of stainless steel, and a support formed of another material can also be used. In the present invention, the support of the stimulable phosphor sheet is preferably formed of a material having a property of attenuating radiation, but is not particularly limited, and any of an inorganic compound material and an organic compound material may be used. Can also be used, with metallic, ceramic or plastic materials being particularly preferred. As an inorganic compound material that can be preferably used to form a support of a stimulable phosphor sheet and can attenuate radiation,
For example, gold, silver, copper, zinc, aluminum, titanium,
Tantalum, chromium, iron, nickel, cobalt, lead, tin,
Metals such as selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz,
Examples thereof include silicon materials such as silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; and inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. These are single crystals,
The polycrystalline sintered body such as amorphous or ceramic may have any structure. Further, as an organic compound material which can be preferably used for forming a support of a stimulable phosphor sheet and which can attenuate radiation,
Polymer compounds are preferably used. Examples of polymer compounds that are preferably used include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon 4,10
Nylon such as; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, Cellulose acetate, Nitrocellulose, Starch, Alginic acid Examples thereof include polysaccharides such as calcium and hydroxypropylmethyl cellulose; chitin; chitosan; sumacum; polyamides such as gelatin, collagen and keratin, and copolymers of these polymer compounds. These may be composite materials,
If necessary, metal oxide particles, glass fibers and the like can be filled, and an organic compound material can be blended and used.

Further, in the above-described embodiment, by using the scanner shown in FIGS. 8 to 15, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are recorded. The radiological data of the radiolabeled substance and the fluorescence data of the fluorescent substance such as the fluorescent dye recorded in the biochemical analysis unit 1 are read to generate the biochemical analysis data. It is not always necessary to read the fluorescence data of the fluorescent substance by one scanner, and the radiation data of the radiolabeled substance and the fluorescence data of the fluorescent substance should be read by separate scanners to generate the data for biochemical analysis. May be.

Further, in the above-mentioned embodiment, the radiological data and the biochemical analysis unit 1 of the radiolabeled substance recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10. Although the scanner shown in FIGS. 8 to 15 is used to read the fluorescence data of the fluorescent substance such as the fluorescent dye recorded in the above to generate the biochemical analysis data, the radiation data of the radiolabeled substance is used. Alternatively, as a scanner for reading the fluorescence data of the fluorescent substance, a large number of dot-shaped stimulable phosphor layer regions 12 or the surface of the biochemical analysis unit 1 are scanned with the laser beam 24 or the excitation light to stimulate the stimulable substance. Any substance capable of exciting a fluorescent substance or a fluorescent substance may be used, and the emission of the radiolabeled substance using the scanner shown in FIG. 8 to FIG. It is not necessarily required to read fluorescence data of the data or the fluorescent substance.

Further, the scanner shown in FIGS. 8 to 15 includes a first laser excitation light source 21, a second laser excitation light source 22 and a third laser excitation light source 23, but three laser excitation light sources are used. It is not always necessary to have a light source.

In the above embodiment, the data generation system capable of generating the fluorescence data shown in FIGS. 16 to 19 also records the data on a large number of absorptive regions 4 formed in the biochemical analysis unit 1. The data for biochemical analysis is generated by reading the chemiluminescence data of the labeling substance that produces chemiluminescence by contact with the chemiluminescence substrate prepared above. It is not always necessary that the data generation system to generate can also generate fluorescence data, and the data generation system exclusively uses the chemistry recorded in a large number of absorptive regions 4 formed in the biochemical analysis unit 1. When used to read only the chemiluminescent data of a labeling substance that produces chemiluminescence upon contact with a luminescent substrate, L D light source 100, filter 101, it is possible to omit the filter 102 and the diffusion plate 103.

In the above embodiment, the optical head 3 is moved by the scanning mechanism in the main scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG.
5 is moved to scan all dot-shaped stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 or the entire surface of the biochemical analysis unit 1 with the laser light 24, thereby stimulating the stimulable phosphor. Alternatively, although a fluorescent substance such as a fluorescent dye is excited, the optical head 35 is kept stationary, and the stage 40 is moved in the main scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG. All the dot-shaped stimulable phosphor layer regions 1 of the stimulable phosphor sheet 10 are moved by the laser light 24 by moving.
2 or scan the entire surface of the biochemical analysis unit 1,
A fluorescent substance such as a stimulable fluorescent substance or a fluorescent dye may be excited, and the optical head 35 may be used as shown in FIG.
In the main scanning direction indicated by arrow X or arrow Y
It is possible to move the stage 40 in the sub-scanning direction indicated by arrow Y or in the main-scanning direction indicated by arrow X while moving in the sub-scanning direction indicated by.

Further, in the scanner shown in FIGS. 8 to 15, a photomultiplier 50 is used as a photodetector to detect fluorescence or stimulated emission photoelectrically. The photodetector used is not limited to the photomultiplier 50 and may be a line CCD as long as it can photoelectrically detect fluorescence or photostimulated light.
Other photodetectors such as a two-dimensional CCD or the like can also be used.

Further, in the above-mentioned embodiment, the spotting device provided with the injector 6 and the CCD camera 7 is used, and the tip of the injector 6 and the specific binding substance such as cDNA should be dropped by the CCD camera 7. While observing the region 4, when the tip of the injector 6 and the center of the adsorptive region 4 to which the specific binding substance such as cDNA should be dropped match the specific binding substance such as cDNA from the injector 6. Although ejected and dropped, the relative positional relationship between the tip of the injector 6 and the many absorptive regions 4 formed in the biochemical analysis unit 1 is detected in advance, and the injector 6 And the biochemical analysis unit 1 are relatively two-dimensionally moved at a constant pitch, and a specific binding substance such as cDNA is dropped. It is also possible Unisuru.

[0296]

INDUSTRIAL APPLICABILITY According to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. is spotted on a carrier. By dropping, a high-density spot-like region was formed, and a substance of biological origin labeled with a radioactive labeling substance was specifically bound to the spot-like specific binding substance, and obtained by selective labeling. The biochemical analysis unit is brought into close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed to a radioactive labeling substance, and the stimulable phosphor layer is irradiated with excitation light to produce a stimulable fluorescent substance. Photostimulation of the photostimulable light emitted from the body layer to generate biochemical analysis data, and when analyzing a substance derived from a living body, the electron beam (β-ray) emitted from the radiolabeled substance is also detected. Prevents noise due to scattering from being generated in biochemical analysis data. Providing unit and a manufacturing method thereof biochemical analysis can become possible.

Further, according to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like is spotted on a carrier. By dripping, a high-density spot-shaped region is formed, and chemiluminescence is generated by contacting the spot-like specific binding substance with a chemiluminescent substrate in addition to the radiolabeling substance or in place of the radiolabeling substance. A substance derived from a living body, which is labeled with a labeling substance and / or a fluorescent substance to be generated, is specifically bound, and chemiluminescence and / or fluorescence emitted from a biochemical analysis unit obtained by selectively labeling is photoelectrically converted. To generate data for biochemical analysis,
Even when a substance derived from a living body is analyzed, chemiluminescence emitted from a labeling substance and / or a fluorescent substance which causes chemiluminescence by contacting with a chemiluminescent substrate and / or
Alternatively, it is possible to provide a biochemical analysis unit and a method for manufacturing the biochemical analysis unit, which can prevent noise caused by fluorescence scattering from being generated in the biochemical analysis data.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a schematic partial cross-sectional view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 3 is a schematic cross-sectional view of a calendering apparatus for manufacturing a biochemical analysis unit.

FIG. 4 is a schematic front view of a spotting device.

FIG. 5 is a schematic cross-sectional view of a hybridization container.

FIG. 6 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 7 shows a large number of dot-shaped stimulable phosphor layers formed on a stimulable phosphor sheet by a radioactive labeling substance contained in a large number of absorptive regions formed in a biochemical analysis unit. It is a schematic sectional drawing which shows the method of exposing an area | region.

FIG. 8 is a schematic perspective view showing an example of a scanner.

FIG. 9 is a schematic perspective view showing details of a scanner near the photomultiplier.

10 is a schematic cross-sectional view taken along the line AA of FIG.

11 is a schematic cross-sectional view taken along the line BB of FIG.

FIG. 12 is a schematic cross-sectional view taken along the line CC of FIG.

FIG. 13 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 14 is a schematic plan view of a scanning mechanism of an optical head.

15 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner shown in FIG.

FIG. 16 is a diagram showing the results obtained by reading chemiluminescence data of a labeling substance that causes chemiluminescence by contacting with a chemiluminescence substrate recorded in a large number of adsorptive regions formed in a biochemical analysis unit, 1 is a schematic front view of a data generation system that generates chemical analysis data.

FIG. 17 is a cooling CCD of the data generation system.
It is a schematic longitudinal cross-sectional view of a camera.

FIG. 18 is a schematic vertical sectional view of a dark box of the data generation system.

FIG. 19 is a block diagram around a personal computer of the data generation system.

FIG. 20 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 21 is a schematic partial cross-sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 22 is a schematic cross-sectional view of a hot press processing apparatus.

FIG. 23 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 24 is a diagram showing a stimulable phosphor sheet formed by a radiolabel substance contained in a large number of absorptive regions formed in a biochemical analysis unit according to another preferred embodiment of the present invention. It is a schematic sectional drawing which shows the method of exposing many dot-shaped photostimulable phosphor layer areas.

FIG. 25 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 26 is a schematic partial cross-sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical analysis unit 2 Adsorbent membrane 3 adhesive 4 Adsorbable area (through hole) 5 Aluminum plate 6 injectors 7 CCD camera 8 Hybridization container 9 Hybridization solution 10 Storage phosphor sheet 11 Support 12 Dot-shaped stimulable phosphor layer region 13 recess 15 calendar rolls 21 First laser excitation light source 22 Second laser excitation light source 23 Third Laser Excitation Light Source 24 laser light 25 Collimator lens 26 mirror 27 First Dichroic Mirror 28 Second dichroic mirror 29 mirror 30 collimator lens 31 Collimator lens 32 mirror 33 holes for the perforated mirror 34 perforated mirror 35 Optical head 36 mirror 37 Aspherical lens 38 concave mirror 40 stages 41 glass plate 45 Fluorescence or stimulated emission 48 filter units 50 Photomultiplier 51a, 51b, 51c, 51d filter member 52a, 52b, 52c, 52d filters 53 A / D converter 54 Data processing device 60 substrates 61 Sub-scanning pulse motor 62 a pair of rails 63 Movable substrate 64 rod 65 Main scanning stepping motor 66 endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 keyboard 72 Filter unit motor 81 Cooled CCD camera 82 Dark Box 83 personal computer 84 CRT 85 keyboard 86 CCD 87 heat transfer plate 88 Peltier element 89 shutter 90 A / D converter 91 Image data buffer 92 Camera control circuit 95 glass plate 96 radiation fin 97 camera lens 100 LED light source 101 Filter 102 filters 103 Diffuser 110 CPU 111 data transfer means 112 data storage means 113 Data processing device 114 data display means 115 Light source control means 121 Biochemical analysis unit 122 adsorptive membrane 123 adhesive 124 Adsorbable area (through hole) 125 plastic substrate 127 base 128 press plate 130 Storage phosphor sheet 131 support 132 Dot-shaped stimulable phosphor layer region 141 Biochemical analysis unit 142 Adsorbent membrane 144 Adsorbent area 145 Occlusion area

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 33/543 521 G01N 33/543 521 33/566 33/566 37/00 102 37/00 102 (72) Inventor Katsuhiro Koda 798, Miyadai, Kaisei-cho, Ashigaragami-gun, Kanagawa Fuji Photo Film Co., Ltd. F-term (reference) 2G043 AA01 BA16 CA03 DA02 EA01 FA01 FA06 GA06 GA07 GB01 GB21 HA01 JA03 LA03 NA06 2G045 AA40 FA11 FB02 FB4 FB15 FB08 FB08 FB03 FB08 FB08 FB03 FB08 FB03 FB08 FB08 AA02 AA06 CE02 EA01 EA03 GA04 GE01 4B029 AA07 AA23 AA27 BB15 BB17 BB20 CC03 FA15

Claims (48)

[Claims] 1. A plate-shaped member formed of a material for attenuating radiation and / or light and having a plurality of through holes is embedded in an absorptive film formed of an absorptive material, and the plurality of through-holes are formed. A biochemical analysis unit characterized in that a plurality of absorptive regions are formed at positions corresponding to the holes. 2. A plate-like member formed of a material that attenuates radiation and / or light and having a plurality of through holes is embedded in an adsorbent film formed of an adsorbent material, and the plurality of through-holes are formed. A plurality of absorptive regions are formed at positions corresponding to the pores, and specific binding substances having known structures or characteristics are dropped onto the plurality of absorptive regions to be adsorbed, and radioactive labeling substances, fluorescent substances and chemical substances are added. The specific substance in which a substance of biological origin labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a luminescent substrate is adsorbed in the plurality of absorptive regions A unit for biochemical analysis, which is specifically bound to a binding substance, and the plurality of absorptive regions are selectively labeled. 3. The specific substance is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor / ligand. The biochemical analysis unit described in. 4. The biochemistry according to claim 1, wherein the plate-like member is press-fitted into the adsorptive film to form the plurality of adsorptive regions. Analysis unit. 5. The biochemical analysis according to claim 4, wherein the plate-shaped member is press-fitted into the adsorptive membrane by a hot pressing process to form the plurality of porous regions. Unit. 6. The biochemical analysis according to claim 5, wherein the plate-shaped member is press-fitted into the adsorptive film by calender roll treatment to form the plurality of adsorptive regions. unit. 7. The plate-shaped member is press-fitted into the absorptive film via an adhesive to form the plurality of absorptive regions. The unit for biochemical analysis according to the item. 8. When the plate-shaped member transmits the radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions, the radiation and / or
Alternatively, the biochemical analysis unit according to any one of claims 1 to 7, which has a property of attenuating light energy to 1/5 or less. 9. The radiation and / or light when the radiation and / or the light penetrates through the fluid member by a distance equal to the distance between the adsorbing regions adjacent to each other.
The biochemical analysis unit according to claim 8, which has a property of attenuating light energy to 1/10 or less. 10. The energy of radiation and / or light when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to the distance between the adjacent absorptive regions. The biochemical analysis unit according to claim 9, wherein the biochemical analysis unit has a property of attenuating the water to 1/100 or less. 11. The biochemistry according to claim 8, wherein the plate-shaped member is made of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. Analysis unit. 12. The biochemical analysis unit according to claim 11, wherein the plate-shaped member is formed by dispersing metal oxide particles in a plastic material. 13. An adsorbent film formed of an adsorbent material is impregnated with a solution containing colloidal metal particles,
A biochemical analysis unit, characterized in that a shielding region for attenuating radiation and light is formed, and a plurality of absorptive regions are formed by a region of the absorptive film in which the shielding region is not formed. 14. An adsorbent film formed of an adsorbent material is impregnated with a solution containing colloidal metal particles,
A plurality of absorptive regions are formed by a region where the shield region that attenuates radiation and light is formed and the absorptive region of the absorptive film is not formed, and the plurality of absorptive regions have known structures or characteristics. Specific binding substance is dropped, adsorbed, labeled with at least one labeling substance selected from the group consisting of a radioactive labeling substance, a fluorescent substance, and a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate. A substance derived from a living body, to the specific binding substance adsorbed on the plurality of absorptive regions,
A unit for biochemical analysis, which is specifically bound to selectively label the plurality of absorptive regions. 15. The substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor / ligand. The biochemical analysis unit described in. 16. The absorptive film is formed with 10 or more absorptive regions.
5. The biochemical analysis unit according to any one of 5 above. 17. The biochemical analysis unit according to claim 16, wherein 1000 or more of the absorptive regions are formed on the absorptive film. 18. The absorptive film is formed with 10,000 or more absorptive regions.
The biochemical analysis unit described in. 19. The plurality of absorptive regions are respectively
The biochemical analysis unit according to any one of claims 1 to 18, wherein the biochemical analysis unit has a size of less than 5 mm 2. 20. Each of the plurality of absorptive regions,
The biochemical analysis unit according to claim 19, having a size of less than 1 mm 2. 21. Each of the plurality of absorptive regions,
The biochemical analysis unit according to claim 20, having a size of less than 0.1 mm 2. 22. The biochemical analysis unit according to claim 1, wherein the plurality of absorptive regions are formed with a density of 10 pieces / square centimeter or more. 23. The biochemical analysis unit according to claim 22, wherein the plurality of absorptive regions are formed with a density of 1000 pieces / square centimeter or more. 24. The biochemical analysis unit according to claim 23, wherein the absorptive regions are formed with a density of 10,000 pieces / square centimeter or more. 25. The biochemical analysis unit according to any one of claims 1 to 24, wherein the absorptive region is regularly formed. 26. The biochemical analysis unit according to any one of claims 1 to 25, wherein the adsorptive film is formed of a porous material. 27. The biochemical analysis unit according to claim 26, wherein the adsorptive membrane is formed of a carbon porous material or a porous material capable of forming a membrane filter. 28. The biochemical analysis unit according to claim 1, wherein the absorptive film is formed of a fiber material. 29. A plate-shaped member, which is made of a material that attenuates radiation and / or light and has a plurality of through holes, is embedded in the absorptive film formed of the absorptive material, and the plurality of through holes are formed. A method for manufacturing a biochemical analysis unit, comprising forming a plurality of absorptive regions at positions corresponding to. 30. A plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through-holes is embedded in the absorptive film formed of the absorptive material, and the plurality of through-holes are formed. A plurality of absorptive regions are formed at positions corresponding to, a specific binding substance having a known structure or property is dropped onto the plurality of absorptive regions and adsorbed, and then a radiolabeled substance, a fluorescent substance and a chemiluminescent substrate The specific binding substance in which a substance derived from a living organism labeled with at least one labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with In the method for producing a biochemical analysis unit, the plurality of absorptive regions are selectively labeled by specifically binding to. 31. The substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor / ligand. A method for manufacturing the biochemical analysis unit described in. 32. The biochemical analysis according to claim 29, wherein the plate-shaped member is press-fitted into the absorptive film to form the plurality of absorptive regions. Method for manufacturing units. 33. The biochemical analysis unit according to claim 32, wherein the plate-shaped member is press-fitted into the adsorptive film by a hot pressing process to form the plurality of adsorptive regions. Manufacturing method. 34. The biochemical analysis unit according to claim 33, wherein the plate-shaped member is press-fitted into the adsorptive film by calender roll treatment to form the plurality of adsorptive regions. Manufacturing method. 35. The plate-shaped member is press-fitted into the absorptive film via an adhesive to form the plurality of absorptive regions.
A method for manufacturing the biochemical analysis unit according to the item. 36. Radiation and / or light energy when the plate-shaped member transmits radiation and / or light through the plate-shaped member by a distance equal to a distance between the adjacent absorptive regions. The method for manufacturing a biochemical analysis unit according to any one of claims 29 to 35, wherein the method has a property of attenuating ⅕ or less. 37. The method for manufacturing a biochemical analysis unit according to claim 36, wherein the plate-shaped member is formed of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. 38. The method for manufacturing a biochemical analysis unit according to claim 37, wherein the plate-shaped member is formed by dispersing metal oxide particles in plastic. 39. An adsorbent film formed of an adsorbent material is impregnated with a solution containing metal colloid single particles,
A method for manufacturing a biochemical analysis unit, characterized in that a shield region for attenuating radiation and light is formed, and a plurality of absorptive regions are formed by a region of the absorptive film in which the shield region is not formed. . 40. An adsorbent film formed of an adsorbent material is impregnated with a solution containing single particles of metal colloid,
Forming a shielding region that attenuates radiation and light, and forming a plurality of absorptive regions by the region of the absorptive film in which the shielding region is not formed, the plurality of absorptive regions having a structure or characteristics. Drop a known specific binding substance,
A substance derived from a living body, which is labeled with at least one labeling substance selected from the group consisting of a labeling substance that causes chemiluminescence by being adsorbed and brought into contact with a radioactive labeling substance, a fluorescent substance and a chemiluminescent substrate, A method for manufacturing a biochemical analysis unit, which specifically binds to the specific binding substance adsorbed in the absorptive region to selectively label the plurality of absorptive regions. 41. The biologically-derived substance is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, an antigen-antibody reaction, and a receptor / ligand. A method for manufacturing the biochemical analysis unit described in. 42. The absorptive film is formed with 10 or more of the absorptive regions.
1. The method for manufacturing the biochemical analysis unit according to any one of 1. 43. Each of the plurality of absorptive regions,
The method for manufacturing a biochemical analysis unit according to claim 29, wherein the biochemical analysis unit is formed in a size of less than 5 mm 2. 44. The method for manufacturing a biochemical analysis unit according to claim 29, wherein the plurality of absorptive regions are formed at a density of 10 pieces / square centimeter or more. 45. The method for manufacturing a biochemical analysis unit according to claim 29, wherein the absorptive region is regularly formed. 46. The method of manufacturing a biochemical analysis unit according to claim 29, wherein the adsorptive film is formed of a porous material. 47. The method for manufacturing a biochemical analysis unit according to claim 46, wherein the adsorptive membrane is formed of a carbon porous material or a porous material capable of forming a membrane filter. 48. The method for manufacturing a biochemical analysis unit according to claim 29, wherein the adsorptive film is formed of a fibrous material.