JP2003014774A - Unit for biochemical analysis and its producing method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a unit for biochemical analysis obtained by bonding a substance derived from an organism labeled by a radioactive labeling substance to a spot-like specific bonding substance and labeling selectively even when a high density spot-like area is formed by dripping a specific bonding substance having known basic arrangement, basic length, composition, and the like, in spot shape onto a carrier in which stimulable light emitted from a stimulable phosphor layer by applying the biochemical analysis unit tightly thereto, exposing the stimulable phosphor layer by the radioactive labeling substance and irradiating it with exciting light can be detected photoelectrically with high resolution and biochemical analysis data exhibiting excellent quantitativeness can be generated. SOLUTION: The unit for biochemical analysis comprises an adsorptive film 4 formed of an adsorptive material wherein the adsorptive film exhibits higher liquid permeability in the direction perpendicular to the film surface as compared with the direction parallel with the film surface.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a biochemical analysis unit and a method for producing the same, more specifically, it is capable of specifically binding to a substance of biological origin, and
A specific binding substance with a known base sequence, base length, composition, etc., is dropped onto the carrier in spots to form a high-density spot-shaped region A biochemical analysis unit obtained by selectively binding a substance of biological origin labeled with a radiolabeled substance and selectively labeling it is brought into close contact with the stimulable phosphor layer to give a stimulable fluorescent substance. Exposing the body layer with a radiolabel,
By irradiating the stimulable phosphor layer with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer, the stimulable light can be detected with high resolution. , Spot-like specific binding substance, in addition to radioactive labeling substance,
Alternatively, instead of the radiolabeled substance, a substance derived from a living body labeled with a labeling substance and / or a fluorescent substance that causes chemiluminescence by contacting with a chemiluminescent substrate is specifically bound and selectively labeled. When the chemiluminescence and / or fluorescence emitted from the obtained biochemical analysis unit is photoelectrically detected, the chemiluminescence and / or fluorescence can be detected with high resolution, and the biochemistry is excellent in quantification. The present invention relates to a biochemical analysis unit capable of generating analysis data and a manufacturing method thereof.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Further, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0007] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0008]

However, a membrane filter widely used as a carrier in a macroarray analysis system is capable of specifically binding to a substance of biological origin, and has a base sequence and a base length. , When the specific binding substance whose composition is known is dropped, it is designed so that more specific binding substance can be impregnated and retained, so that the dropped specific binding substance , It is unavoidable that it penetrates into the membrane filter also in the direction parallel to its surface. Therefore, when a specific binding substance is dropped on the membrane filter to form a large number of spot-shaped regions at high density. The specific binding substance contained in a large number of spot-shaped regions can be labeled with a substance of biological origin labeled with a radioactive labeling substance by hybridization or the like. , By specifically binding and selectively labeling the membrane filter with a stimulable phosphor layer-containing stimulable phosphor layer and a stimulable phosphor sheet formed thereon. Exposing the exhaustive phosphor layer,
Then, by irradiating the stimulable phosphor layer with excitation light, photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate biochemical analysis data. There was a problem that the resolution of photodetection was lowered and biochemical analysis data excellent in quantification could not be generated.

Although such a problem can be solved to some extent by reducing the thickness of the membrane filter, when the thickness of the membrane filter is reduced, the specific binding substance impregnated in the membrane filter is reduced. Inevitably, the amount of the biomolecules inevitably decreases, and the substance of biological origin labeled with a radioactive labeling substance is specifically bound to the specific binding substance contained in the spot-like region by hybridization, etc. Labeling, the membrane filter is brought into close contact with the stimulable phosphor sheet in which the stimulable phosphor layer containing the stimulable phosphor is formed, and the stimulable phosphor layer is exposed by a radioactive labeling substance, and after that When irradiating the stimulable phosphor layer with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate biochemical analysis data. Signal intensity produced also becomes inevitably lowered, the accuracy of quantitative analysis is lowered.

Further, as described above, in the field of biochemical analysis, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. formed in spots at different positions on the surface of the membrane filter are used. In addition to radiolabeled substances, specific binding substances that can specifically bind to substances of biological origin, such as proteins, nucleic acids, cDNA, DNA, RNA, and whose base sequence, base length, and composition are known , A substance of biological origin, which is labeled with a labeling substance and / or a fluorescent substance that produces chemiluminescence by contacting with a chemiluminescent substrate, is specifically bound by hybridization or the like, selectively labeled, and radioactively labeled. After exposing the stimulable phosphor layer with a labeling substance, or prior to exposing the stimulable phosphor layer with a radioactive labeling substance. It is emitted from a fluorescent substance by bringing it into contact with a chemiluminescent substrate, photoelectrically detecting chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeling substance, and / or irradiating with excitation light. It is also required to photoelectrically detect fluorescence and analyze a substance derived from a living body, but even in such a case, a large number of spot-shaped regions,
When formed at a high density, the specific binding substance dropped on the membrane filter permeates the inside of the membrane filter in a direction parallel to its surface, and as a result, chemiluminescence and / or Alternatively, there has been a problem that when fluorescence is detected photoelectrically, the resolution of light detection is lowered, and biochemical analysis data excellent in quantification cannot be generated.

Therefore, according to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. is dropped onto a carrier in a spot form. Thus, even when a high-density spot-shaped region is formed, a substance of biological origin labeled with a radiolabeling substance is specifically bound to the spot-shaped specific binding substance, and the substance is selectively labeled. The biochemical analysis unit is placed in close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed to a radioactive labeling substance, and the stimulable phosphor layer is irradiated with excitation light to stimulate the stimulable phosphor layer. When photoelectrically detecting the photostimulable light emitted from the phosphor layer, the photostimulable light can be detected with high resolution, and raw data capable of generating biochemical analysis data with excellent quantitativeness can be generated. To provide a unit for chemical analysis and its manufacturing method. The it is an object of the present invention.

Another object of the present invention is to spot a specific binding substance, which is capable of specifically binding to a substance derived from a living body and whose base sequence, base length, composition, etc. are known, on a carrier. Even when a high-density spot-like region is formed by dropping, by contacting with a chemiluminescent substrate in addition to the radio-labeled substance or in place of the radio-labeled substance, the spot-like specific binding substance Chemiluminescence and / or fluorescence emitted from a biochemical analysis unit obtained by selectively binding a biological substance labeled with a labeling substance and / or a fluorescent substance that generate chemiluminescence, and selectively labeling A biochemical solution capable of detecting chemiluminescence and / or fluorescence at high resolution when photoelectrically detecting a biochemical solution, and capable of generating biochemical analysis data with excellent quantitativeness. To provide a use unit and a manufacturing method thereof.

[0013]

The object of the present invention is to:
An adsorptive film formed of an adsorptive material, wherein the adsorptive film has higher liquid permeability in a direction perpendicular to the film surface as compared to a direction parallel to the film surface. It is achieved by a biochemical analysis unit characterized by.

According to the present invention, since the adsorptive film has higher liquid permeability in the direction perpendicular to the film surface as compared to the direction parallel to the film surface, the surface of the adsorptive film is In addition, when the specific binding substance is dropped in a spot shape to form the spot-shaped region, the specific binding substance is effectively prevented from penetrating in the direction parallel to the film surface of the adsorptive membrane. Therefore, even when the spot-like region of the specific binding substance is formed at a high density, the specific binding substance is hybridized with the substance derived from the living body labeled with the radioactive labeling substance to give the radioactive labeling substance. By selectively labeling with the radioactive labeling substance contained in the spot area,
The exposed photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected with high resolution, and the biochemistry is excellent in quantitativeness. It becomes possible to generate data for analysis.

Further, according to the present invention, the adsorptive film has higher liquid permeability in the direction perpendicular to the film surface as compared with the direction parallel to the film surface. When a specific binding substance is dropped in a spot shape on the surface of to form a spot-shaped area, the specific binding substance is effectively prevented from penetrating in the direction parallel to the surface of the adsorptive membrane. Therefore, the spot-like region of the specific binding substance can be
Even when formed with high density,
A substance derived from a living body labeled with a fluorescent substance and / or a substance derived from a living body labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate are hybridized to selectively form a large number of spot regions. The labeled biochemical analysis unit is irradiated with an excitation hole to excite the fluorescent substance, and the fluorescent substance emitted from the fluorescent substance and / or the chemiluminescent substrate is brought into contact with the chemiluminescent substrate to emit chemiluminescence. It becomes possible to photoelectrically detect chemiluminescence with high resolution and generate biochemical analysis data with excellent quantification.

In a preferred embodiment of the present invention, the adsorptive membrane is composed of a fiber bundle of a plurality of fibers, and most of the plurality of fibers constituting the fiber bundle are
It is oriented in a direction perpendicular to the film surface.

According to a preferred embodiment of the present invention, the adsorptive membrane is composed of a fiber bundle composed of a plurality of fibers,
Since most of the plurality of fibers that make up the fiber bundle are oriented in the direction perpendicular to the film surface, the adsorptive membrane is higher in the direction perpendicular to the film surface than in the direction parallel to the film surface. Since it has liquid permeability, when the specific binding substance is dropped in a spot form on the surface of the adsorptive membrane to form a spot-like region, the specific binding substance is absorbed into the film of the adsorptive membrane. Since it is possible to effectively prevent permeation in the direction parallel to the surface, even when the spot-like region of the specific binding substance is formed at a high density, the specific binding substance is labeled with a radioactive labeling substance. The substance derived from the living body is hybridized and selectively labeled with a radioactive labeling substance, and the exposed stimulable phosphor layer is irradiated with excitation light by the radioactive labeling substance contained in the spot region. Emitted from the stimulable phosphor layer The luminescence light, with high resolution, photoelectrically detected, it is possible to produce biochemical analysis data having an excellent quantitative characteristic.

Further, according to a preferred embodiment of the present invention, the absorptive membrane is composed of a fiber bundle composed of a plurality of fibers, and most of the plurality of fibers constituting the fiber bundle are arranged in a direction perpendicular to the membrane surface. Since it is oriented, the adsorptive membrane has higher liquid permeability in the direction perpendicular to the membrane surface as compared to the direction parallel to the membrane surface, and therefore, the surface of the adsorptive membrane has a specific It is possible to effectively prevent the specific binding substance from penetrating in the direction parallel to the film surface of the adsorptive film when the spot binding region is formed by dropping the specific binding substance. Even when the spot-like region of the specific binding substance is formed at a high density, chemiluminescence can be obtained by bringing the specific binding substance into contact with the substance of biological origin labeled with a fluorescent substance and / or the chemiluminescent substrate. Depending on the labeling substance produced The biochemical analysis unit in which a large number of spot regions are selectively labeled by irradiating the excitation holes to hybridize the labeled substances derived from the living body to excite the fluorescent substances and release them from the fluorescent substances Fluorescence and /
Alternatively, chemiluminescence emitted from a labeling substance that causes chemiluminescence by contact with a chemiluminescent substrate can be detected photoelectrically with high resolution to generate highly quantitative biochemical analysis data. become.

[0019] In a further preferred aspect of the present invention, the fiber bundle is formed by fusing at least a part of the plurality of fibers.

In another preferred embodiment of the present invention, at least a part of the fiber bundle is impregnated with an adhesive.

[0021] In a preferred aspect of the present invention, at least a part of the plurality of fibers has a hollow structure along the axis.

According to a preferred embodiment of the present invention, since at least a part of the plurality of fibers has a hollow structure along the axis, liquid permeability in a direction perpendicular to the membrane surface of the adsorptive membrane is obtained. It becomes possible to further improve
It is possible to reduce the liquid permeability in the direction parallel to the membrane surface, and to more effectively prevent the dropped specific binding substance from penetrating in the direction parallel to the membrane surface of the adsorptive membrane. You can

In a preferred embodiment of the present invention, the absorptive film is filled with a plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through holes, and the plate-shaped member is formed. The member divides the absorptive film into a plurality of absorptive film regions separated from each other,
The plurality of absorptive film regions are formed at positions corresponding to the plurality of through holes of the plate member.

According to a preferred embodiment of the present invention, a plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through holes is embedded in the absorptive film to form a plate-shaped member. The absorptive film is divided into a plurality of absorptive film regions that are spaced apart from each other, and a plurality of absorptive film regions are formed at positions corresponding to the plurality of through holes of the plate-shaped member. When the member is formed of a material having a property of attenuating radiation, a specific binding substance that can specifically bind to a substance derived from a living body and whose base sequence, base length, composition, etc. is known. , A specific binding substance adsorbed on a plurality of absorptive membrane regions formed in the biochemical analysis unit to be adsorbed and labeled with a radioactive labeling substance, which is derived from a living body Specifically tied to By, after selectively labeled, so as to face the biochemical analysis unit in the stimulable phosphor layer, a stimulable phosphor layer during the exposure by a radioactive labeling substance,
A plate-like member formed of a material having a property of attenuating radiation exists around each adsorptive film region, and therefore, an electron beam emitted from the radiolabel substance contained in the adsorptive film region ( Beta rays are scattered, and scattered electron beams (beta rays) are incident on the region of the stimulable phosphor layer to be exposed by the radiolabel substance emitted from the adjacent adsorptive film region. Since it can be surely prevented, the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected. When generating biochemical analysis data and analyzing substances of biological origin, noise is generated in the biochemical analysis data due to scattering of electron beams (β rays) emitted from radiolabeled substances. Can be effectively prevented That.

Further, according to a preferred embodiment of the present invention, when the plate-shaped member is formed of a material having a property of attenuating light, it is capable of specifically binding to a substance of biological origin and a base. Specific binding substances with known sequence, base length, composition, etc. are dropped onto the multiple adsorbent membrane regions formed in the biochemical analysis unit to be adsorbed, and instead of radiolabeled substances, chemiluminescence A substance derived from a living body, which is labeled with a labeling substance and / or a fluorescent substance that produces chemiluminescence when brought into contact with a substrate, is specifically bound to a specific binding substance adsorbed in the adsorptive membrane region, and selected. Chemically labeled and then contacted with a chemiluminescent substrate,
Photoelectrically detecting chemiluminescence in the visible light wavelength region generated by contact between the chemiluminescent substrate and the labeling substance, and / or
A material having the property of attenuating light around each adsorptive membrane region when generating biochemical analysis data by photoelectrically detecting fluorescence emitted from a fluorescent substance by irradiating excitation light. By virtue of the presence of the plate-shaped member formed by the method, it is possible to reliably prevent the scattering of chemiluminescence and / or fluorescence. Therefore, the biochemistry generated by photoelectrically detecting chemiluminescence and / or fluorescence. It is possible to effectively prevent generation of noise in the analysis data due to chemiluminescence and / or fluorescence scattering.

Further, according to a preferred embodiment of the present invention, when the plate-shaped member is made of a material having a property of attenuating radiation and light, it can be specifically bound to a substance derived from a living body, and , A base sequence or a length of a base, a specific binding substance whose composition is known, is dropped onto a plurality of adsorptive membrane regions formed in a biochemical analysis unit, and is adsorbed, in addition to a radiolabeled substance, A labeling substance which produces chemiluminescence by contacting with a chemiluminescent substrate and / or
Or, a substance of biological origin labeled with a fluorescent substance,
After specifically binding to the specific binding substance adsorbed in the adsorptive film region and selectively labeling, the photostimulable phosphor layer is opposed to the stimulable phosphor layer by the radioactive labeling substance. At the time of exposure, since a plate-like member formed of a material having a property of attenuating radiation and light is present around each adsorptive film region, an electron beam (β-ray) emitted from a radioactive labeling substance Reliably prevents scattered electron beams (β-rays) from entering the region of the stimulable phosphor layer to be exposed by the radioactive labeling substance emitted from the adjacent adsorptive film region. Therefore, the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected, and biochemistry is detected. Generates data for analysis and analyzes substances of biological origin In this case, it is possible to effectively prevent the noise caused by the scattering of the electron beam (β-ray) emitted from the radiolabeled substance from being generated in the biochemical analysis data. The substance from which it is specifically bound to the specific binding substance and selectively labeled,
The fluorescence emitted from the fluorescent substance is detected by photoelectrically detecting chemiluminescence in the visible light wavelength region generated by contacting the chemiluminescent substrate with the labeling substance and / or irradiating with excitation light. By photoelectrically detecting and generating biochemical analysis data, a plate-like member formed of a material having a property of attenuating radiation and light is present around each adsorptive film region, It is possible to reliably prevent chemiluminescence and / or fluorescence from scattering,
Therefore, it is possible to effectively prevent generation of noise due to scattering of chemiluminescence and / or fluorescence in the biochemical analysis data generated by photoelectrically detecting chemiluminescence and / or fluorescence. It will be possible.

[0027] In a further preferred aspect of the present invention, the plurality of absorptive film regions are formed by press-fitting the plate-shaped member into the absorptive film.

According to a further preferred embodiment of the present invention, since a plurality of absorptive film regions can be formed by simply press-fitting the plate member into the absorptive film.
It is possible to easily generate a biochemical analysis unit.

[0029] In a further preferred aspect of the present invention, the plurality of absorptive film regions are formed by press-fitting the plate-shaped member into the absorptive film by calendering.

According to a further preferred embodiment of the present invention, a plurality of absorptive film regions can be formed simply by press-fitting the plate-like member into the absorptive film by calendering. Moreover, it is possible to generate the biochemical analysis unit at a low cost.

In a further preferred aspect of the present invention, the plurality of absorptive film regions are formed by press-fitting the plate-shaped member into the absorptive film via an adhesive.

According to a further preferred embodiment of the present invention, the plate-like member is press-fitted into the adsorptive film via an adhesive,
Since the plurality of absorptive film regions are formed, it is possible to firmly integrate the plate-shaped member and the absorptive film.

In a preferred embodiment of the present invention, the substrate further comprises a substrate formed of a radiation and / or light attenuating material and having a plurality of holes, wherein the adsorptive film has a plurality of layers. The pores are filled with the adsorptive material and are formed by a plurality of the adsorptive film regions formed.

According to a preferred embodiment of the present invention, the biochemical analysis unit further comprises a substrate formed of a material that attenuates radiation and / or light and having a plurality of holes, and the adsorptive film is Since the adsorbent material is embedded in the plurality of holes of the substrate and formed by the plurality of adsorbent film regions formed, when the plate member is formed of a material having a property of attenuating radiation, Is
A specific binding substance that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. is known is dropped onto multiple absorptive membrane regions formed in the biochemical analysis unit. Then, the substance of biological origin that has been adsorbed and labeled with a radioactive labeling substance is specifically bound to the specific binding substance adsorbed in the adsorptive membrane region, and selectively labeled, followed by biochemical analysis. When the stimulable phosphor layer is exposed to the radioactive labeling substance by facing the stimulable phosphor layer to the unit for use, the surrounding of each porous region is formed of a material having a property of attenuating radiation. There is a plate-shaped member,
Therefore, the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive film region is scattered, and the photosensitivity to be exposed by the radio-labeled substance emitted from the adjacent adsorptive film region. Since it is possible to reliably prevent scattered electron beams (β-rays) from entering the region of the phosphor layer, irradiate the stimulable phosphor layer exposed with the radioactive labeling substance with excitation light. , Photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor layer to generate biochemical analysis data, and when analyzing a substance derived from a living body, an electron beam emitted from a radiolabeled substance It is possible to effectively prevent the noise due to the scattering of (β-rays) from being generated in the biochemical analysis data.

Further, according to a preferred embodiment of the present invention, when the plate-shaped member is made of a material having a property of attenuating light, it is capable of specifically binding to a substance derived from a living body and a base. Specific binding substances with known sequence, base length, composition, etc. are dropped onto the multiple adsorbent membrane regions formed in the biochemical analysis unit to be adsorbed, and instead of radiolabeled substances, chemiluminescence A substance derived from a living body labeled with a labeling substance and / or a fluorescent substance, which causes chemiluminescence when brought into contact with a substrate, is specifically bound to a specific binding substance adsorbed in the porous region,
After selectively labeling, it is brought into contact with a chemiluminescent substrate, photoelectrically detecting chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeling substance, and / or irradiated with excitation light, A plate-like member formed of a material having a property of attenuating light around each adsorptive film region when photoelectrically detecting fluorescence emitted from a fluorescent substance and generating biochemical analysis data. Since it is possible to prevent chemiluminescence and / or fluorescence from scattering, it is possible to prevent chemiluminescence and / or fluorescence from photoelectrically detecting chemiluminescence and / or fluorescence. It becomes possible to effectively prevent generation of noise due to scattering of light emission and / or fluorescence.

Furthermore, according to a preferred embodiment of the present invention, when the plate-shaped member is formed of a material having a property of attenuating radiation and light, it can bind specifically to a substance of biological origin, and , A base sequence or a length of a base, a specific binding substance whose composition is known, is dropped onto a plurality of adsorptive membrane regions formed in a biochemical analysis unit, and is adsorbed, in addition to a radiolabeled substance, A labeling substance which produces chemiluminescence by contacting with a chemiluminescent substrate and / or
Or, a substance of biological origin labeled with a fluorescent substance,
After specifically binding to the specific binding substance adsorbed in the porous region and selectively labeling, the photostimulable phosphor layer is exposed to the radioactive labeling substance by facing the photostimulable phosphor layer. In doing so, since a plate-like member formed of a material having a property of attenuating radiation and light is present around each adsorptive film region, an electron beam (β-ray) emitted from the radiolabeled substance is present. Be sure to prevent scattered electron beams (β rays) from entering the region of the stimulable phosphor layer that is to be exposed by the radioactive labeling substance that is scattered and emitted from the adjacent adsorptive film region. Therefore, the photostimulable phosphor layer exposed by the radiolabeled substance is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. Data for analysis and analysis of substances of biological origin In this case, it is possible to effectively prevent the noise caused by the scattering of the electron beam (β-ray) emitted from the radiolabeled substance from being generated in the data for biochemical analysis. Substance is specifically bound to a specific binding substance, selectively labeled, and then contacted with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by contact between the chemiluminescent substrate and the labeling substance is photoelectrically generated. Detection and / or irradiation of excitation light to photoelectrically detect the fluorescence emitted from the fluorescent substance and generate biochemical analysis data. The presence of the plate-shaped member formed of a material having the property of attenuating radiation and light can reliably prevent the scattering of chemiluminescence and / or fluorescence, and thus can prevent chemiluminescence and / or fluorescence. The biochemical during analysis data produced by photoelectrically detecting noise due to chemiluminescence and / or scattering of fluorescence it is possible to effectively prevented from being generated.

In a further preferred aspect of the present invention, a plurality of through holes are formed in the substrate, and the plurality of absorptive film regions are provided in the plurality of through holes formed in the substrate. Are embedded and formed.

In another preferred aspect of the present invention, a plurality of recesses are formed in the substrate, and the plurality of absorptive film regions are provided with an absorptive material in the plurality of recesses formed in the substrate. It is embedded and formed.

The above object of the present invention is also a method for manufacturing a biochemical analysis unit having an adsorptive membrane, wherein a fiber bundle comprising a plurality of fibers, most of the plurality of fibers are the absorptive membrane. This is achieved by a method for manufacturing a biochemical analysis unit, characterized in that the adsorptive film is formed by arranging so as to face in a direction perpendicular to the film surface.

According to the present invention, a fiber bundle composed of a plurality of fibers is arranged so that most of the plurality of fibers are oriented in a direction perpendicular to the film surface of the absorptive film to form the absorptive film. Since the biochemical analysis unit is manufactured by the method described above, the adsorptive membrane of the biochemical analysis unit has higher liquid permeability in the direction perpendicular to the membrane surface as compared with the direction parallel to the membrane surface. Therefore, when a specific binding substance is dropped on the surface of the adsorptive film in a spot shape to form a spot-shaped region, the specific binding substance is directed in a direction parallel to the film surface of the adsorptive film. Can effectively prevent the penetration of
Even when the spot-like region of the specific binding substance is formed at a high density, the specific binding substance is hybridized with a substance of biological origin labeled with a radiolabeling substance, and selectively labeled with the radiolabeling substance. Then, by the radioactive labeling substance contained in the spot region, the exposed photostimulable phosphor layer, irradiated with excitation light, the photostimulable light emitted from the photostimulable phosphor layer, with high resolution, It becomes possible to generate data for biochemical analysis which is detected photoelectrically and has excellent quantitativeness.

Further, according to the present invention, the fiber bundle composed of a plurality of fibers is arranged so that most of the plurality of fibers are oriented in the direction perpendicular to the film surface of the absorptive film, and the absorptive film is formed. Since the biochemical analysis unit is manufactured by the formation, the adsorptive membrane of the biochemical analysis unit has a higher liquid permeation in the direction perpendicular to the membrane surface as compared with the direction parallel to the membrane surface. Therefore, when the specific binding substance is dropped on the surface of the adsorptive film in a spot shape to form a spot-like region, the specific binding substance is absorbed into the film of the adsorptive film. Since it can be effectively prevented from penetrating in the direction parallel to the surface, even when the spot-like region of the specific binding substance is formed at a high density, the specific binding substance is labeled with the fluorescent substance. Biological material and / or chemical emissions A substance derived from a living body that is labeled with a labeling substance that produces chemiluminescence when brought into contact with a substrate is hybridized, and a biochemical analysis unit in which a large number of spot regions are selectively labeled is irradiated with an excitation hole. Chemiluminescence emitted from a labeling substance that excites a fluorescent substance and causes chemiluminescence by contacting with fluorescence emitted from the fluorescent substance and / or a chemiluminescent substrate, photoelectrically detecting with high resolution, It becomes possible to generate biochemical analysis data with excellent quantitativeness.

In a preferred embodiment of the present invention, at least a part of the plurality of fibers is fused to form the fiber bundle.

In another preferred embodiment of the present invention, at least a part of the plurality of fibers is impregnated with an adhesive to form the fiber bundle.

In a preferred embodiment of the present invention, at least a part of the plurality of fibers has a hollow structure along the axis.

According to a preferred embodiment of the present invention, since at least a part of the plurality of fibers has a hollow structure along the axis, liquid permeability in a direction perpendicular to the membrane surface of the adsorptive membrane is obtained. It becomes possible to further improve
It is possible to reduce the liquid permeability in the direction parallel to the membrane surface, and to more effectively prevent the dropped specific binding substance from penetrating in the direction parallel to the membrane surface of the adsorptive membrane. You can

[0046] In a preferred aspect of the present invention, the absorptive film is formed by embedding a plate-like member formed of a material that attenuates radiation and / or light and having a plurality of through holes therein. In addition, a plurality of absorptive membrane regions that are spaced apart from each other and located at positions corresponding to the plurality of through holes of the plate-like member are formed to generate a biochemical analysis unit.

In a further preferred aspect of the present invention, the plate member is press-fitted into the adsorptive film to form the plurality of adsorptive film regions.

According to a further preferred embodiment of the present invention, since a plurality of porous regions are formed by simply press-fitting the plate-shaped member into the adsorptive membrane, the biochemical analysis unit can be easily used. It will be possible to generate.

In a further preferred aspect of the present invention, the plate-shaped member is press-fitted into the adsorptive film by calendering to form the plurality of adsorptive film regions.

According to a further preferred embodiment of the present invention, a plurality of porous regions are formed by simply press-fitting the plate-shaped member into the adsorptive membrane by calendering, so that it is easy and inexpensive. Then, it becomes possible to generate the unit for biochemical analysis.

In a further preferred aspect of the present invention, the plate member is press-fitted into the adsorptive film via an adhesive to form the plurality of adsorptive film regions. .

According to a further preferred embodiment of the present invention, the plate-like member is press-fitted into the adsorptive film via an adhesive,
Since the plurality of porous regions are formed, it becomes possible to firmly integrate the plate-shaped member and the adsorptive film.

In a preferred embodiment of the present invention, a substrate formed of a material that attenuates radiation and / or light and provided with a plurality of holes is further prepared, and the plurality of holes formed in the substrate are provided with: It is configured to embed an adsorbent material to form a plurality of adsorbent film regions to form the adsorbent film.

In a further preferred aspect of the present invention, a plurality of through holes are formed in the substrate, and an adsorptive material is embedded in the plurality of through holes formed in the substrate to form the plurality of adsorbent films. Is configured to form a region.

In another preferred aspect of the present invention, a plurality of recesses are formed in the substrate, and an adsorbent material is embedded in the recesses formed in the substrate to form the plurality of adsorbent film regions. Are configured to form.

In a preferred aspect of the present invention, the absorptive membrane region is regularly formed.

In a preferred embodiment of the invention 1
Zero or more of the absorptive membrane regions are formed.

In a further preferred aspect of the present invention, 100 or more of the absorptive membrane regions are formed.

[0059] In a further preferred aspect of the present invention, 1000 or more of the absorptive membrane regions are formed.

In a further preferred embodiment of the present invention, 10,000 or more of the absorptive membrane regions are formed.

[0061] In a further preferred aspect of the present invention, 100,000 or more of the absorptive membrane regions are formed.

In a preferred embodiment of the present invention, each of the plurality of adsorptive membrane regions is formed to have a size of less than 5 mm 2.

[0063] In a further preferred aspect of the present invention, each of the plurality of adsorbent film regions is formed to have a size of less than 1 mm 2.

[0064] In a further preferred aspect of the present invention, each of the plurality of adsorbent film regions is formed to have a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of adsorbent film regions is formed to have a size of less than 0.1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of adsorptive membrane regions is formed to have a size of less than 0.05 mm 2.

In a further preferred aspect of the present invention, each of the plurality of adsorptive membrane regions is formed to have a size of less than 0.01 mm 2.

In a preferred embodiment of the present invention, the plurality of absorptive membrane regions are formed at a density of 10 pieces / square centimeter or more.

In a further preferred aspect of the present invention, the plurality of adsorbent film regions are formed at a density of 50 or more per cm 2.

[0070] In a further preferred aspect of the present invention, the plurality of adsorbent film regions are formed at a density of 100 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive film regions are formed at a density of 500 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive membrane regions are formed at a density of 1,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive membrane regions are formed at a density of 5000 pieces / square centimeter or more.

In a further preferred aspect of the present invention, the plurality of adsorbent film regions are formed at a density of 10,000 or more per cm 2.

In the present invention, a fibrous material or a porous material is preferably used as the adsorptive material, and a fibrous material is particularly preferable. A fibrous material and a porous material can be used together to form the adsorptive region.

The fiber material used in the present invention is not particularly limited, but preferably, for example, nylons such as nylon 6, nylon 6,6 and nylon 4,10, nitrocellulose, cellulose acetate,
Examples thereof include cellulose derivatives such as cellulose butyrate acetate.

The porous material used in the present invention is
Either an organic material or an inorganic material may be used, and an organic / inorganic composite may be used.

The organic porous material used in the present invention is not particularly limited, but a carbon material such as activated carbon or a porous material capable of forming a membrane filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6 and nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid / polylysine polyion complex, etc. Examples thereof include alginic acids; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluorides such as polytetrafluoride, and copolymers or composites thereof.

The inorganic porous material used in the present invention is not particularly limited, but preferably, for example, metals such as platinum, gold, iron, silver, nickel and aluminum; alumina, silica, titania, Examples thereof include metal oxides such as zeolite; metal salts such as hydroxyapatite and calcium sulfate, and composites thereof.

In a preferred embodiment of the present invention, the radiation- and / or light-attenuating material is such that the radiation and / or light penetrates the material by a distance equal to the distance between adjacent adsorbent membrane regions. When transmitted, it has a property of attenuating the energy of radiation and / or light to ⅕ or less.

In a further preferred embodiment of the present invention, the radiation and / or light attenuating material is
The radiation and / or light energy is reduced by 1/1 when the radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent membrane regions.
It has the property of being attenuated to 0 or less.

In a further preferred embodiment of the present invention, the radiation and / or light attenuating material is
Radiation and / or light energy is reduced by 1/5 when radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent membrane regions.
It has the property of being attenuated to 0 or less.

In a further preferred embodiment of the present invention, the radiation and / or light attenuating material is
The radiation and / or light energy is reduced by 1/1 when the radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent membrane regions.
It has the property of being attenuated to 00 or less.

In a further preferred embodiment of the present invention, the radiation and / or light attenuating material is
Radiation and / or light energy is reduced by 1/5 when radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent membrane regions.
It has the property of being attenuated to 00 or less.

In a further preferred embodiment of the present invention, the radiation and / or light attenuating material is
The radiation and / or light energy is reduced by 1/1 when the radiation and / or light penetrates through the material by a distance equal to the distance between adjacent adsorbent membrane regions.
It has the property of being attenuated to 000 or less.

In the present invention, the material for forming the plate-shaped member or the substrate is not particularly limited as long as it has a property of attenuating radiation and / or light, and it is an inorganic compound material or an organic material. Although any compound material can be used, metallic materials, ceramic materials or plastic materials are preferably used.

In the present invention, as the inorganic compound material capable of attenuating radiation and / or light which can be preferably used for forming the plate member or the substrate, for example, gold, silver, copper, zinc, aluminum, Titanium, tantalum, chromium, iron, nickel, cobalt, lead,
Metals such as tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; tungsten. Inorganic salts such as carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material capable of attenuating radiation and / or light, and radiation and / or that can be preferably used for forming a plate-shaped member or substrate. Examples of the polymer compound capable of attenuating light include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; Polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylon 6, nylon 6,
Nylon such as nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenylsiloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer;
Polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan; sumac;
Examples thereof include polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds. These may be composite materials, if necessary, it is possible to fill with metal oxide particles or glass fibers,
Also, an organic compound material can be blended and used.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the plate member or the substrate has a specific gravity of 1.
It is preferably formed of a compound material or composite material of 0 g / cm ³ or more, and particularly preferably formed of a compound material or composite material having a specific gravity of 1.5 g / cm ³ or more and 23 g / cm ³ or less.

On the other hand, in general, the greater the light scattering and / or absorption, the higher the light attenuating ability. Therefore, the plate member or the substrate preferably has an absorbance of 0.3 or more per cm of thickness. More preferably, the absorbance per cm of thickness is 1 or more. Where the absorbance is
It is determined by placing an integrating sphere immediately after the plate having a thickness of Tcm, measuring the transmitted light amount A at the wavelength of probe light or emission light used for measurement with a spectrophotometer, and calculating A / T. . In the present invention, in order to improve the light attenuating ability, a light-scattering body or a light-absorbing body may be contained in the plate member or the substrate. Fine particles of a material different from the material forming the plate member or the substrate are used as the light scatterer, and a pigment or dye is used as the light absorber.

[0091]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention, and FIG. 2 is a partial sectional view thereof.

As shown in FIGS. 1 and 2, the biochemical analysis unit 1 according to the present embodiment is provided with an absorptive film 2 formed of nylon 6.

In this embodiment, the absorptive membrane 2 bundles a plurality of nylon 6 fibers and impregnates a part thereof with an adhesive to produce a nylon 6 fiber bundle. It is formed by slicing along the direction orthogonal to the axis of the. Therefore, nylon 6
Most of the fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 2.

FIG. 3 is a process chart showing an example of a method for producing the adsorptive film 2.

As shown in FIG. 3 (A), an adhesive, for example, a hot melt adhesive 2b is impregnated into an appropriate portion of the surface of the fiber 2a of each nylon 6.

Next, as shown in FIG. 3B, a nylon 6 fiber bundle produced by bundling a large number of nylon 6 fibers 2a is oriented in a direction perpendicular to the axis of the many nylon 6 fibers 2a. Then, a compressed body 2c of nylon 6 having a rectangular cross section is formed by being compressed from a large number of nylon 6 fibers.

As shown in FIG. 3 (C), the compressed body 2c thus produced is sliced to a predetermined thickness along the plane orthogonal to the axis of many fibers 6a of nylon 6 to form an adsorbent film. 2 is produced.

As shown in FIGS. 1 and 2, the absorptive film 2 has an aluminum substrate 5 having a property of attenuating radiation and light and having a large number of substantially circular through holes formed regularly. The calendering device press-fits the aluminum substrate 5, so that a large number of the absorptive film regions 4 are regularly formed in a dot shape corresponding to the large number of through holes of the aluminum substrate 5. Here, the adhesive 3 is applied to the back surface of the aluminum substrate 5, and the aluminum substrate 5 is press-fitted into the adsorptive film 2 via the adhesive 3. Therefore, the aluminum substrate 5 is the adsorptive film. It is firmly integrated with the 2 to improve the durability.

Although not shown exactly in FIG. 1, in the present embodiment, a substantially circular adsorbent membrane region 4 having a size of about 0.01 mm 2 of about 100,000.
Are regularly formed in the biochemical analysis unit 1 at a density of about 1000 pieces / square centimeter.

As shown in FIG. 2, in the present embodiment, the aluminum substrate 5 is placed so that the surface of the adsorbent film region 4 and the surface of the aluminum substrate 5 are located at the same height. The biochemical analysis unit 1 is formed by press-fitting into the biochemical analysis unit 1.

FIG. 4 is a schematic sectional view of a calendering apparatus for manufacturing the biochemical analysis unit 1.

The calendering apparatus is equipped with a pair of calender rolls 15 whose temperature can be controlled.
And an aluminum substrate 5 to which the adhesive 3 is applied on the lower surface, and the aluminum substrate 5 is supplied between a pair of calender rolls 15 and pressed at a predetermined pressure, whereby the adsorptive film 2 is provided. The aluminum substrate 5 is press-fitted to correspond to the through holes regularly formed in the aluminum substrate 5,
The biochemical analysis unit 1 in which a large number of adsorptive film regions 4 are regularly formed is manufactured. The pressure applied from the calender rolls 15 can be arbitrarily determined according to the material of the adsorptive film 2, the degree to which the aluminum substrate 5 is press-fitted, the temperature conditions, and the like.

FIG. 5 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 5, in a large number of adsorbent film regions 4 regularly formed in the biochemical analysis unit 1, for example, a base sequence as a specific binding substance is used. Known different cDNs
A is dropped using a spotting device.

As shown in FIG. 5, the spotting device comprises an injector 6 and a CCD camera 7, and a CCD
By the camera 7, the tip of the injector 6 and the cDN
While observing the adsorptive film region 4 to which A is dropped, when the tip of the injector 6 and the center of the adsorptive film region 4 to which the cDNA is dropped match,
The cDNA is configured to be released and dropped into the adsorptive membrane region 4, so that the cDNA can be accurately dropped into the many adsorptive membrane regions 4 formed in the biochemical analysis unit 1. Guaranteed to be able to.

In this embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are absent in the absorptive membrane 2. Since the adsorptive membrane 2 is arranged in the direction perpendicular to the surface, the adsorptive membrane 2 has a higher liquid permeability in the direction perpendicular to the surface as compared with the liquid permeability in the direction parallel to the surface. It is possible to effectively prevent the specific binding substance dropped in the adsorptive membrane regions 4 from penetrating in the direction parallel to the surface of the adsorptive membranes 2 and reaching the adjacent adsorbent membrane regions 4. .

Further, in the present embodiment, in the adsorptive film region 4 of the biochemical analysis unit 1, the aluminum substrate 5 having a large number of through holes regularly formed is press-fitted into the adsorptive film 2, Since it is formed, the voids in the adsorptive film 2 between the adjacent adsorptive film regions 4 have disappeared due to the pressurization, and therefore, the specific binding substance dropped in the adsorptive film region 4 is , Is effectively prevented from penetrating in the direction parallel to the surface of the adsorptive membrane 2 and reaching the adjacent adsorbent membrane regions 4, and adsorbs the specific binding substance dropped in the adsorbent membrane regions 4. It can be adsorbed only to the film region 4.

FIG. 6 is a schematic cross-sectional view of the hybridization container.

As shown in FIG. 6, the hybridization container 8 has a rectangular cross section, and contains a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with a labeling substance. .

In the case of selectively labeling a specific binding substance such as cDNA with a radioactive labeling substance, a hybridization solution 9 containing a substance derived from a living body which is a probe labeled with a radioactive labeling substance is prepared and It is housed in the hybridization container 8.

On the other hand, by using a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, the cDNA is
In the case of selectively labeling a specific binding substance such as, a hybridization solution 9 containing a substance derived from a living body, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, is prepared. And is housed in the hybridization container 8.

Furthermore, when a specific binding substance such as cDNA is selectively labeled with a fluorescent substance such as a fluorescent dye, a high-molecular substance containing a probe which is a probe labeled with a fluorescent substance such as a fluorescent dye is used. A hybridization solution 9 is prepared and housed in the hybridization container 8.

[0114] A substance derived from a living body labeled with a radioactive labeling substance, a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, and a living body labeled with a fluorescent substance such as a fluorescent dye It is also possible to prepare a hybridization solution 9 containing two or more substances derived from the living body among the substances derived from the living body, and store the hybridization solution 9 in the hybridization container 8. In the present embodiment, the living body labeled with the radioactive labeling substance is used. A hybridization solution 9 containing a substance derived from a living body, a substance derived from a living body labeled with a fluorescent substance such as a fluorescent dye, and a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is prepared. And is accommodated in the hybridization container 8.

Upon hybridization, cD
The biochemical analysis unit 1 in which a specific binding substance such as NA is adsorbed on a large number of adsorptive membrane regions 4 is inserted into the hybridization container 8.

As a result, the specific binding substance adsorbed on the large number of adsorptive membrane regions 4 is labeled with a radioactive labeling substance and is contained in the hybridization solution 9 and is a biological substance or a fluorescent substance such as a fluorescent dye. Labeled by
The biological substance contained in the hybridization solution 9 and the biological substance labeled with the labeling substance that causes chemiluminescence by contacting with the chemiluminescent substrate are selectively hybridized.

Here, in this embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is composed of a plurality of nylons 6.
Since many of the plurality of nylon 6 fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 2, the adsorptive membrane 2 is a liquid in a direction parallel to the surface. The permeability of the liquid in the direction perpendicular to the surface is higher than that of the specific binding substance. Therefore, the specific binding substance dropped in the adsorptive membrane region 4 is in the direction parallel to the surface of the adsorptive membrane 2. It is possible to effectively prevent the permeation and reach the adjacent adsorbing membrane regions 4 and to adsorb the specific binding substance dropped in the adsorbing membrane regions 4 only to the adsorbing membrane regions 4. Therefore, the specific binding substance dropped onto the adsorptive membrane region 4 permeates into the adjacent adsorptive membrane regions 4, and the specific binding substance permeates into the adjacent adsorptive membrane regions 4, the radiolabeled substance, Chemiluminescence by contact with a fluorophore and a chemiluminescent substrate Substance derived from a living organism and labeled it becomes possible to reliably prevent the hybridizing to a labeling substance which generates.

Further, in the present embodiment, the adsorptive film region 4 of the biochemical analysis unit 1 is press-fitted into the adsorptive film 2 with the aluminum substrate 5 having a large number of through holes formed regularly. Since it is formed, the voids in the adsorptive film 2 between the adjacent adsorptive film regions 4 have disappeared due to the pressurization, so that the specific binding substance dropped in the adsorptive film region 4 is , Which effectively prevent the permeation in the direction parallel to the surface of the adsorptive membrane 2 to reach the adjacent adsorbent membrane regions 4, and the specific binding substance dropped in the adsorbent membrane regions 4 is Since it can be adsorbed only to the adsorptive membrane region 4, the specific binding substance dropped onto the adsorptive membrane region 4 permeates into the adsorbent membrane regions 4 adjacent to each other and permeates into the adsorbent membrane regions 4 adjacent to each other. The specific binding substances are Substance derived from a living organism and labeled with a labeling substance which generates chemiluminescent emission when it contacts a chemiluminescent substrate becomes possible to reliably prevent the hybridizing.

In this way, by contacting a large number of adsorptive membrane regions 4 of the biochemical analysis unit 1 with the radiation data of the radioactive labeling substance which is the labeling substance, the fluorescence data of the fluorescent substance such as the fluorescent dye and the chemiluminescent substrate. The chemiluminescence data of the labeling substance that causes chemiluminescence is recorded. The fluorescence data recorded in the absorptive film area 4 is read by a scanner described later to generate biochemical analysis data, while the chemiluminescence data recorded in the absorptive film area 4 is a data generation system described later. The data is read by a cooled CCD camera of the above and data for biochemical analysis is generated.

On the other hand, the radiation data of the radio-labeled substance is transferred to the stimulable phosphor sheet, and the radiation data transferred to the stimulable phosphor sheet is read by the scanner described later for biochemical analysis. Data is generated.

FIG. 7 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 7, the stimulable phosphor sheet 10 is provided with a support 11, and one side of the support 11 is provided with a large number of adsorptive films formed in the biochemical analysis unit 1. A large number of recesses 13 are formed in the same regular pattern as the pattern of the region 4, and the stimulable phosphor is embedded in the plurality of recesses 13 to form a large number of substantially circular dot-shaped stimulable phosphors. A layer region 12 is formed.

In this embodiment, the support 11 is made of stainless steel having the property of attenuating radiation, and the large number of recesses 13, and thus the substantially circular dot-shaped stimulable phosphor layer region 12, are It is formed on the support 11 so as to have the same diameter as the adsorptive membrane region 4 formed on the biochemical analysis unit 1.

Also, the dot-shaped stimulable phosphor layer region 12 is formed.
The photostimulable phosphor is embedded in the recess 13 so that the surface of the substrate and the surface of the support 11 are located at the same height, and the dot-shaped photostimulable phosphor layer region 12 is formed. There is.

FIG. 8 shows a large number of dot-shaped photostimulable substances formed on the stimulable phosphor sheet 10 by the radioactive labeling substance contained in the large number of absorptive film regions 4 formed in the biochemical analysis unit 1. FIG. 6 is a schematic cross-sectional view showing a method of exposing the phosphor layer region 12 to light.

As shown in FIG. 8, upon exposure,
The many absorptive film regions 4 formed in the biochemical analysis unit 1 face the many dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. Then, the stimulable phosphor sheet 10 and the biochemical analysis unit 1 are superposed.

In the present embodiment, since the biochemical analysis unit 1 is formed by press-fitting the aluminum substrate 5 into the adsorptive film 2, even if it is subjected to liquid treatment such as hybridization, Therefore, the large number of adsorptive film regions 4 formed in the biochemical analysis unit 1 do not expand or contract, and therefore, the stimulable phosphor sheet 10 does not.
The stimulable phosphor sheet 10 and the biochemical analysis unit 1 are easily and surely arranged so as to accurately face the large number of dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of FIG. And the dot-shaped stimulable phosphor layer region 12
Can be exposed.

Thus, over a predetermined time, each of the dot-shaped stimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and the biochemical analysis unit 1 are formed. By arranging a large number of the absorptive film regions 4 to face each other, a large number of dot-shaped stimulable phosphor layer regions formed on the stimulable phosphor sheet 10 by the radioactive labeling substance contained in the absorptive film regions 4 are formed. 12 is exposed.

At this time, an electron beam is emitted from the radioactive labeling substance adsorbed on the adsorptive film region 4, but the adsorptive film region 4 of the biochemical analysis unit 1 attenuates the radiation on the adsorptive film 2. The aluminum substrate 5 having the property of causing a large number of through holes is press-fitted to be formed, and the aluminum substrate 5 having the property of attenuating radiation exists around the adsorptive film region 4. Therefore, the adsorptive membrane area 4
The electron beam emitted from the radio-labeled substance contained in is surely prevented from scattering, and all the electron beams emitted from the radio-labeled substance contained in the adsorptive film region 4 are contained in the adsorptive film. The photostimulable phosphor layer region 12 which is to be exposed to the electron beam incident on the photostimulable phosphor layer region 12 facing the region 4 and emitted from the adsorbing film region 4 adjacent thereto.
It is possible to surely prevent the light from entering.

Furthermore, in the present embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is composed of a plurality of nylons 6.
Since many of the plurality of nylon 6 fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 2, the adsorptive membrane 2 is a liquid in a direction parallel to the surface. The permeability of the liquid in the direction perpendicular to the surface is higher than that of the specific binding substance. Therefore, the specific binding substance dropped in the adsorptive membrane region 4 is directed in the direction parallel to the surface of the adsorptive membrane 2. Since the permeation can be effectively prevented and the specific binding substance dropped in the adsorptive film region 4 can be adsorbed only to the adsorptive film region 4,
The specific binding substance dropped onto the permeate the adsorbing membrane regions 4 adjacent to each other, and as a result of hybridization of the substance of biological origin labeled with the radioactive labeling substance, the electrons to be released from the adsorbing membrane region 4 It is possible to reliably prevent the line from being emitted from the adsorbing film regions 4 adjacent to each other and exposing the stimulable phosphor layer region 12 arranged to face the adsorbing film regions 4 adjacent to each other. .

Further, in the present embodiment, the adsorptive film region 4 of the biochemical analysis unit 1 is press-fitted into the adsorptive film 2 with the aluminum substrate 5 having a large number of through holes regularly formed, Since it is formed, the voids in the adsorptive film 2 between the adjacent adsorptive film regions 4 have disappeared due to the pressurization, and therefore, the specific binding substance dropped in the adsorptive film region 4 is , The specific binding substance dropped in the adsorptive membrane region 4 can be effectively prevented by permeating into the regions of the adsorptive membrane region 4 other than the adsorptive membrane region 4 only. Since it can be adsorbed, the adsorptive film region 4
The specific binding substance dropped onto the permeate the adsorbing membrane regions 4 adjacent to each other, and as a result of hybridization of the substance of biological origin labeled with the radioactive labeling substance, the electrons to be released from the adsorbing membrane region 4 It is possible to reliably prevent the line from being emitted from the adsorbing film regions 4 adjacent to each other and exposing the stimulable phosphor layer region 12 arranged to face the adsorbing film regions 4 adjacent to each other. .

Therefore, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are contained in the corresponding absorptive film region 4 of the biochemical analysis unit 1 and are labeled with a radioactive substance. Only by doing so, it is possible to reliably perform exposure.

Thus, the radiation data of the radioactive labeling substance is recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10.

FIG. 9 shows a large number of dot-shaped stimulable phosphor layer regions 1 formed on the support 11 of the stimulable phosphor sheet 10.
2 of the scanner for generating the biochemical analysis data by reading the radiation data of the radiolabeled substance recorded in 2 and the fluorescence data of the fluorescent dye or the like recorded in the many adsorptive film regions 4 of the biochemical analysis unit 1. FIG. 9 is a schematic perspective view showing an example, and FIG. 9 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner shown in FIG. 9 has radiation data and biochemistry of a radiolabel substance recorded in a large number of dot-shaped stimulable phosphor layer regions 12 formed on a support 11 of a stimulable phosphor sheet 10. The first laser excitation light source 21 and 532 that are configured to be able to read fluorescence data such as a fluorescent dye recorded in a large number of adsorptive film regions 4 of the analysis unit 1 and emit laser light 24 having a wavelength of 640 nm.
A second laser excitation light source 22 that emits a laser light 24 having a wavelength of nm and a third laser excitation light source 23 that emits a laser light 24 having a wavelength of 473 nm are provided. In the present embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source, and the second laser excitation light source 22 and the third laser excitation light source 23 are second harmonic generation (Seco).
nd Harmonic Generation) element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. First
In the optical path of the laser light 24 emitted from the laser excitation light source 21 and reflected by the mirror 26, the first dichroic mirror 27 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer A second dichroic mirror 28 that transmits light of a wavelength and reflects light of a wavelength of 473 nm is provided.
Laser light 24 generated by the laser excitation light source 21 of
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated by the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, it is reflected by the first dichroic mirror 27, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 28 and enters the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is made into parallel light by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

The laser beam 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser beam 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 or the biochemical analysis unit 1 placed on 41.

When the laser beam 24 is incident on the dot-shaped stimulable phosphor layer area 12 of the stimulable phosphor sheet 10, it is included in the dot-shaped stimulable phosphor layer area 12 formed on the stimulable phosphor sheet 10. When the photostimulable phosphor present in the biochemical analysis unit 1 is excited to emit photostimulable light 45 and the laser beam 24 enters the biochemical analysis unit 1, the adsorptive film region 4 formed in the biochemical analysis unit 1 is detected. The fluorescent dye or the like contained in is excited and the fluorescence 45 is emitted.

Photostimulation 45 emitted from the dot-shaped stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 or fluorescence 45 emitted from the absorptive film region 4 formed in the biochemical analysis unit 1. Is condensed on a mirror 36 by an aspherical lens 37 provided in the optical head 35, is reflected by the mirror 36 on the same side as the optical path of the laser light 24, becomes parallel light, and is incident on a concave mirror 38. To do.

The photostimulable light 45 or fluorescent light 45 that has entered the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror and enters the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 10, the filter unit 48 includes four filter members 51a, 51b, 51.
c and 51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 10 by a motor (not shown).

FIG. 11 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 11, the filter member 5
1a includes a filter 52a, and the filter 52a includes a first
A filter used when a fluorescent substance such as a fluorescent dye contained in the adsorptive film region 4 formed in the biochemical analysis unit 1 is excited by using the laser excitation light source 21 of FIG. It is a member, and has a property of cutting light having a wavelength of 640 nm and transmitting light having a wavelength longer than 640 nm.

FIG. 12 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 12, the filter member 5
1b includes a filter 52b, and the filter 52b includes a second
The filter used when the fluorescent substance such as a fluorescent dye contained in the adsorptive film region 4 formed in the biochemical analysis unit 1 is excited by using the laser excitation light source 22 of FIG. It is a member and has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 13 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 13, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
A filter used when the fluorescent substance such as a fluorescent dye contained in the adsorptive film region 4 formed in the biochemical analysis unit 1 is excited by using the laser excitation light source 23 of FIG. It is a member, and has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 14 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 14, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
It is used when exciting the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 formed in 0 to read the stimulable light 45 emitted from the stimulable phosphor layer region 12. The filter has a property of transmitting only light in the wavelength range of stimulable light emitted from the stimulable phosphor layer 12 and cutting light of a wavelength of 640 nm.

Therefore, the filter members 51a, 51b, 51c, 51d are selected depending on the laser excitation light source to be used.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

Although not shown in FIG. 9, the optical head 35 is configured to be movable in the X direction and the Y direction in FIG. 9 by the scanning mechanism, and the stimulable phosphor sheet 1 is formed.
All the dot-shaped stimulable phosphor layer regions 1 formed in 0
2 or the entire surface of the biochemical analysis unit 1 is configured to be scanned by the laser beam 24.

FIG. 15 is a schematic plan view of the scanning mechanism of the optical head. In FIG. 15, for simplification, the optical system excluding the optical head 35 and the optical paths of the laser light 24 and the stimulated emission 45 or the fluorescence 45 are omitted.

As shown in FIG. 15, the optical head 35
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
And 6 are fixed on the substrate 60 and are movable in the sub-scanning direction indicated by arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in the hole. Engaged.

A main scanning stepping motor 65 is provided on the movable substrate 63, and the main scanning stepping motor 65 connects the endless belt 66 to the adsorbing film regions 4 adjacent to each other formed in the biochemical analysis unit 1. With a pitch equal to the distance between, and thus the stimulable phosphor sheet 10
It is configured such that it can be driven intermittently at a pitch equal to the distance between adjacent photostimulable phosphor layer regions 12 formed on the support 11. The optical head 35 includes the endless belt 6
When the endless belt 66 is driven by the main scanning stepping motor 65 which is fixed to FIG.
In the main scanning direction indicated by the arrow X in FIG.

In FIG. 15, 67 is the optical head 35.
Is a linear encoder for detecting the position in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the main scanning stepping motor 6
5, the endless belt 66 is driven in the main scanning direction, and when the scanning of one line is completed, the substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61, whereby the optical head 35. 15 is moved in the X-Y direction in FIG. 15 and all the dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 or the entire surface of the biochemical analysis unit 1 by the laser light 24. Are scanned.

FIG. 16 is a block diagram showing the control system, input system, drive system and detection system of the scanner shown in FIG.

As shown in FIG. 16, the scanner control system includes a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 that is operated by an operator and can input various instruction signals.

As shown in FIG. 16, the scanner drive system intermittently moves the optical head 35 in the main scanning direction and the main scanning stepping motor 65, and intermittently moves the optical head 35 in the sub scanning direction. Sub-scanning pulse motor 6
1 and 4 filter members 51a, 51b, 51c, 5
A filter unit motor 72 for moving the filter unit 48 including 1d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

As shown in FIG. 16, the detection system of the scanner includes a photomultiplier 50 and a linear encoder 67 for detecting the position of the optical head 35 in the main scanning direction.

In the present embodiment, the control unit 70 controls the first laser pumping light source 21, the second laser pumping light source 22 or the third laser pumping light source 22 according to the position detection signal of the optical head 35 input from the linear encoder 67. The laser excitation light source 23 is configured to be on / off controllable.

The scanner configured as described above uses the radiolabeling substance contained in the large number of adsorptive film regions 4 formed in the biochemical analysis unit 1 in the following manner to form a large number of dots. The stimulable phosphor layer region 12 of 1 is exposed to read the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 10 to generate biochemical analysis data.

First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40 so that a large number of dot-shaped photostimulable phosphor layer regions 12 are in contact with the surface of the glass plate 41.

Next, the user operates the keyboard 7
An instruction signal for scanning a large number of dot-shaped photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 with a laser beam 24 is input to the display device 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
Photostimulable light 45 emitted from the photostimulable phosphor by moving 8
The filter member 51d provided with the filter 52d having the property of transmitting only the light in the wavelength range of 640 nm and cutting the light of the wavelength of 640 nm is positioned in the optical path of the stimulated emission light 45.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, A position where the first dot-shaped stimulable phosphor layer region 12 among the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 can be irradiated with the laser beam 24. When it is confirmed that the optical head 35 has reached,
A stop signal is output to the main scanning stepping motor 65, and a drive signal is output to the first laser excitation light source 21 to activate the first laser excitation light source 21, and 640 nm
The laser light 24 of the wavelength is emitted.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, and then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser beam 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 onto the first dot-shaped stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41. It

As a result, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 is excited by the laser beam 24 to generate the first stimulable phosphor. The photostimulable light 45 is emitted from the photostimulable phosphor layer region 12.

The photostimulable light 45 emitted from the first dot-shaped photostimulable phosphor region 12 is condensed by the aspherical lens 37 provided in the optical head 35, and the laser light 24 is reflected by the mirror 36. The light is reflected on the same side as the optical path, becomes parallel light, and enters the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 34 is reflected by the concave mirror 38.
Incident on.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and is incident on the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d transmits only the light in the wavelength region of the photostimulable light 45 emitted from the photostimulable phosphor, and emits 640n.
Since it has a property of cutting off the light of wavelength m, the light of wavelength 640 nm which is the excitation light is cut off and the wavelength of photostimulable light 45 emitted from the dot-shaped photostimulable phosphor layer region 12 Only the light in the region passes through the filter 52d and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

After the first laser excitation light source 21 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 70 outputs a drive stop signal to the first laser excitation light source 21, The drive of the first laser excitation light source 21 is stopped, and a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move adjacent dots formed on the support 11 of the stimulable phosphor sheet 10. The stimulable phosphor layer regions 12 are moved by a pitch equal to the distance between the regions.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adjacent dot-shaped stimulable phosphor layer regions 12 to form the laser light 24 emitted from the first laser excitation light source 21 on the stimulable phosphor sheet 10. When it is confirmed that the second dot-shaped photostimulable phosphor layer region 12 thus irradiated has been moved to a position where it can be irradiated, the control unit 70 outputs a drive signal to the first laser excitation light source 21. The stimulability contained in the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 by turning on the first laser excitation light source 21 and the laser light 24. Excite the phosphor.

Similarly, the laser beam 24 is applied to the second dot-shaped stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10 for a predetermined time, and the second stimulable phosphor layer 12 is irradiated. The stimulable phosphor contained in the stimulable phosphor layer region 12 is excited, and the stimulable light 45 emitted from the second stimulable phosphor layer region 12 is converted by the photomultiplier 50 into
When photoelectrically detected and analog data is generated,
The control unit 70 includes the first laser excitation light source 2
1 to turn off the first laser excitation light source 21, and the main scanning stepping motor 65
Then, a drive signal is output to set the optical head 35 to a distance equal to the distance between adjacent dot-shaped photostimulable phosphor layer regions 12.
Move only the pitch.

In this way, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and when it is confirmed that the scanning of the dot-shaped stimulable phosphor layer region 12 of the first line by the laser light 24 is completed, the control unit 70 Outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to its original position and outputs a drive signal to the sub scanning pulse motor 61 to move the movable substrate 63 to the sub scanning direction. Then, move only one line.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line is moved by one line in the sub-scanning direction, the control unit 70 sequentially applies the first laser excitation to the dot-shaped stimulable phosphor layer region 12 of the first line. Laser light emitted from the first laser excitation light source 21 is sequentially applied to the dot-shaped stimulable phosphor layer region 12 of the second line in the same manner as the irradiation of the laser light 24 emitted from the light source 21. 24 to irradiate 24 to excite the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 to sequentially emit stimulable light 45 emitted from the stimulable phosphor layer region 12. , Photomultiplier 50,
It is detected photoelectrically.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processing device 54.

In this way, all the many dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are scanned by the laser light 24 emitted from the first laser excitation light source 21, and many When the stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 12 is excited,
The emitted photostimulable light 45 is photoelectrically detected by the photomultiplier 50, and the generated analog data is converted into digital data by the A / D converter 53 and sent to the data processing device 54. A drive stop signal is output from the control unit 70 to the first laser excitation light source 21, and the drive of the first laser excitation light source 21 is stopped.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the large number of adsorptive film regions 4 formed in the biochemical analysis unit 1 is read to generate the biochemical analysis digital data, first, the operator Thus, the biochemical analysis unit 1 is set on the glass plate 41 of the stage 40.

Next, the operator specifies on the keyboard 71 the type of the fluorescent substance as the labeling substance and inputs an instruction signal to read the fluorescent data.

The instruction signal input to the keyboard 71 is
When the control unit 70 receives the instruction signal, the control unit 70 determines the laser excitation light source to be used according to the table stored in the memory (not shown), and the filter 52.
Which of a, 52b, and 52c is to be positioned in the optical path of the fluorescent light 45 is determined.

For example, Rhodamine (registered trademark) which can be most efficiently excited by a laser having a wavelength of 532 nm as a fluorescent substance for labeling a substance derived from a living body
Is used and is input to the keyboard 71, the control unit 70 selects the second laser excitation light source 22, selects the filter 52b, and outputs a drive signal to the filter unit motor 72. Then, the filter unit 48 is moved to cut the light having the wavelength of 532 nm, and the filter member 51b having the property of transmitting the light having the wavelength longer than 532 nm is emitted from the biochemical analysis unit 1. It is located in the optical path of the fluorescent light 45 to be formed.

Further, the control unit 70 outputs a drive signal to the main scanning stepping motor 65 to move the optical head 35 in the main scanning direction, and based on the position detection signal of the optical head 35 input from the linear encoder, Of the many absorptive film regions 4 formed in the biochemical analysis unit 1, the laser beam 2 is applied to the first absorptive film region 4.
When it is confirmed that the optical head 35 has reached the position where 4 can be irradiated, a stop signal is output to the main scanning stepping motor 65 and a drive signal is output to the second laser excitation light source 22, The second laser excitation light source 22 is activated to emit the laser light 24 having a wavelength of 532 nm.

The laser light 24 emitted from the second laser excitation light source 22 is made into parallel light by the collimator lens 30, and then enters the first dichroic mirror 27 and is reflected.

The laser beam 24 reflected by the first dichroic mirror 27 passes through the second dichroic mirror 28 and enters the mirror 29.

The laser light 24 incident on the mirror 29 is reflected by the mirror 29, and further incident on the mirror 32 and reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 on the biochemical analysis unit 1 mounted on the stage 40 glass plate 41.

As a result, the laser beam 24 excites a fluorescent substance such as a fluorescent dye contained in the first adsorptive film region 4 of the biochemical analysis unit 1, for example, rhodamine to emit fluorescence.

Here, in the biochemical analysis unit 1 according to this embodiment, the absorptive membrane region 4 has the absorptive membrane 2
In addition, the aluminum substrate 5 having a property of attenuating radiation and light and having a large number of through holes formed therein is press-fitted and formed, and a property of attenuating the radiation and light is provided around the adsorptive film region 4. Since the aluminum substrate 5 is included, the fluorescent substance contained in the adsorptive film regions 4 is excited, and the fluorescence 45 emitted from the fluorescent substance is included in the adjacent adsorptive film regions 4. Can be reliably prevented from being excited and mixed with the emitted fluorescence 45.

The adsorptive membrane region 4 of the biochemical analysis unit 1 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are perpendicular to the surface of the absorptive membrane 2. Since they are arranged in the direction, the adsorptive membrane 2
Has a higher liquid permeability in the direction perpendicular to the surface than the liquid permeability in the direction parallel to the surface thereof. Therefore, the specific binding substance dropped in the adsorptive membrane region 4 is
The specific binding substance dropped in the adsorptive membrane region 4 is effectively prevented by penetrating in the direction parallel to the surface of the adsorptive membrane 2 and effectively preventing it from reaching the adjacent adsorptive membrane regions 4. Since it can be adsorbed only to the adsorptive membrane region 4, the specific binding substance dropped onto the adsorptive membrane region 4 permeates into the adjacent adsorbent membrane regions 4 and permeates into the adjacent adsorbent membrane regions 4. As a result of hybridization of the specific binding substance with a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a radioactive labeling substance, a fluorescent substance and a chemiluminescent substrate, It is possible to reliably prevent the fluorescence 45 to be emitted from being emitted from the adsorbing film regions 4 adjacent to each other.

In addition, in the present embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is obtained by press-fitting the aluminum substrate 5 in which a large number of through holes are regularly formed into the adsorptive membrane 2. The voids in the adsorbent film 2 between the adsorbent film regions 4 adjacent to each other have disappeared due to the pressurization, so that the specific binding substance dropped in the adsorbent film region 4 was formed. Penetrates in a direction parallel to the surface of the adsorptive membrane 2 and effectively prevents the adsorbents from reaching the adjacent adsorbent membrane areas 4, and the specific binding substance dropped in the adsorbent membrane areas 4 is prevented. Since it can be adsorbed only to the absorptive membrane region 4, the specific binding substance dropped on the absorptive membrane region 4 permeates the absorptive membrane regions 4 adjacent to each other and the absorptive membrane regions 4 adjacent to each other. Specific binding substances that have penetrated into the As a result of hybridization of a substance derived from a living body, which is labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate, fluorescence 45 to be emitted from the adsorptive membrane region 4 is adjacent to the adsorbent membrane region. It is possible to reliably prevent the release from the above.

The fluorescent light 45 emitted from the rhodamine is collected by the aspherical lens 37 provided in the optical head 35, reflected by the mirror 36 to the same side as the optical path of the laser light 24, and made into parallel light. , Enters the concave mirror 38.

The fluorescence 45 which has entered the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.

The fluorescence 45 which has entered the perforated mirror 34 is
As shown in FIG. 9, the light is reflected downward by the perforated mirror 34 formed by the concave mirror and enters the filter 52b of the filter unit 48.

Since the filter 52b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm, which is excitation light, is cut and emitted from rhodamine. Fluorescence 45
Only the light in the wavelength range of 1 passes through the filter 52b and is photoelectrically detected by the photomultiplier 50.

The analog signal photoelectrically detected and generated by the photomultiplier 50 is output to the A / D converter 53, converted into a digital signal, and output to the data processing device 54.

When a predetermined time, for example, several microseconds elapses after the second laser excitation light source 22 is turned on, the control unit 70 outputs a drive stop signal to the second laser excitation light source 22, The drive of the second laser excitation light source 22 is stopped, and a drive signal is output to the main scanning stepping motor 65 to cause the optical head 35 to move between the absorptive film regions 4 formed in the biochemical analysis unit 1. Move a pitch equal to the distance.

Based on the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is moved by one pitch equal to the distance between the adsorbing membrane regions 4 formed adjacent to each other in the biochemical analysis unit 1,
Laser light 24 emitted from the second laser excitation light source 22
Is confirmed to have moved to a position where the second adsorptive film region 4 formed in the biochemical analysis unit 1 can be irradiated, the control unit 70 sends a drive signal to the second laser excitation light source 22. To output the second laser excitation light source 2
2 is turned on, and the laser beam 24 excites a fluorescent substance, for example, rhodamine, contained in the second adsorptive film region 4 formed in the biochemical analysis unit 1.

Similarly, the laser beam 24 is applied to the second absorptive film region 4 formed in the biochemical analysis unit 1 for a predetermined time and is emitted from the second absorptive film region 4. When the fluorescent light 45 is photoelectrically detected by the photomultiplier 50 and analog data is generated, the control unit 70 outputs an off signal to the second laser excitation light source 22, and the second laser excitation light source 22 is turned off. The excitation light source 22 is turned off, and a drive signal is output to the main scanning stepping motor 65 so that the optical head 35 is equal to the distance between adjacent adsorbable film regions 4 formed in the biochemical analysis unit 1. Move only the pitch.

In this way, the first laser excitation light source 21 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 35, and the optical head 35 is detected based on the position detection signal of the optical head 35 input from the linear encoder 67. 35 is moved by one line in the main scanning direction, and all the absorptive membrane regions 4 of the first line of the biochemical analysis unit 1 are
When the scanning is confirmed by the laser light 24,
The control unit 70 outputs a drive signal to the main scanning stepping motor 65 to return the optical head 35 to the original position, and outputs a drive signal to the sub scanning pulse motor 61 to move the movable substrate 63. The line is moved by one line in the sub-scanning direction.

On the basis of the position detection signal of the optical head 35 input from the linear encoder 67, the optical head 35
Is returned to its original position, and the movable substrate 63 is
When it is confirmed that the line has been moved by one line in the sub-scanning direction, the control unit 70 causes the biochemical analysis unit 1 to form the adsorbable film region 4 on the first line.
In the same manner as irradiating the laser light 24 emitted from the second laser excitation light source 22 in sequence, the second line of the absorptive film region 4 formed in the biochemical analysis unit 1 is sequentially exposed. , The laser beam 24 emitted from the second laser excitation light source 22 is irradiated, and the absorptive film region 4 of the second line
Rhodamine contained in the is excited, and the fluorescence 45 emitted from the adsorptive film region 4 is sequentially photoelectrically detected by the photomultiplier 50.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

In this way, all the absorptive film regions 4 formed in the biochemical analysis unit 1 are scanned by the laser light 24 emitted from the second laser excitation light source 22,
Rhodamine contained in all the adsorptive film regions 4 formed in the biochemical analysis unit 1 is excited, and the emitted fluorescence 45 is photoelectrically detected by the photomultiplier 50 to generate an analog. The data is A / D
Converted into digital data by the converter 53,
When sent to the data processing device 54, a drive stop signal is sent from the control unit 70 to the second laser excitation light source 2
2 and the driving of the second laser excitation light source 22 is stopped.

FIG. 17 shows the results obtained by reading the chemiluminescence data of the labeling substance that causes chemiluminescence by contacting with the chemiluminescence substrate recorded in the large number of adsorptive membrane regions 4 formed in the biochemical analysis unit 1. FIG. 1 is a schematic front view of a data generation system that generates biochemical analysis data. Figure 1
The data generation system shown in 6 is also configured to be able to generate fluorescence data of a fluorescent substance such as a fluorescent dye recorded in a large number of adsorptive film regions 4 formed in the biochemical analysis unit 1.

As shown in FIG. 17, the data generation system comprises a cooled CCD camera 81, a dark box 82 and a personal computer 83. The personal computer 83 includes a CRT 84 and a keyboard 85.

FIG. 18 shows the cooling CC of the data generation system.
4 is a schematic vertical sectional view of a D camera 81. FIG.

As shown in FIG. 18, the cooled CCD camera 81 has a CCD 86, a heat transfer plate 87 made of metal such as aluminum, a Peltier element 88 for cooling the CCD 86, and a front surface of the CCD 86. Shutter 89, A / D converter 90 for converting analog data generated by CCD 86 into digital data, and A / D converter 90
A data buffer 91 for temporarily storing the data digitized by the / D converter 90 and a camera control circuit 92 for controlling the operation of the cooled CCD camera 81 are provided. An opening formed between the dark box 82 and the dark box 82 is closed by a glass plate 95, and a radiation fin 96 for radiating heat generated by the Peltier element 88 is provided around the cooling CCD camera 81 in the longitudinal direction. It is formed over the entire surface.

A camera lens 97 having a lens focus adjusting function is mounted in the dark box 82 in front of the glass plate 95.

FIG. 19 shows the dark box 82 of the data generation system.
FIG.

As shown in FIG. 19, an LED light source 100 that emits excitation light is provided in the dark box 82, and the LED light source 100 is provided with a filter 101 that is detachably provided and an upper surface of the filter 101. The diffuser plate 103 provided is provided, and the excitation light passes through the diffuser plate 103.
Biochemical analysis unit mounted on it (not shown)
By irradiating the biochemical analysis unit, the biochemical analysis unit is guaranteed to be uniformly irradiated. The filter 101 has a property of cutting light harmful to the excitation of the fluorescent substance other than the wavelength near the excitation light and transmitting only the light of the wavelength near the excitation light. On the front surface of the camera lens 97, a filter 102 that cuts light having a wavelength near the excitation light is provided.
Is detachably provided.

FIG. 20 is a block diagram around the personal computer 83 of the data generating system.

As shown in FIG. 20, the personal computer 83 has a CPU 110 for controlling the exposure of the cooled CCD camera 81, a data transfer means 111 for reading the digital data generated by the cooled CCD camera 81 from the data buffer 91, and a digital signal. On the screen of the CRT 84, based on the data storage means 112 for storing data, the data processing device 113 for performing data processing on the digital data stored in the data storage means 112, and the digital data stored in the data storage means 112. The data display means 114 which displays visible data is provided.

The LED light source 100 has a light source control means 115.
The light source control unit 115 is configured to receive an instruction signal from the keyboard 85 via the CPU 110. The CPU 110 is configured to be able to output various signals to the camera control circuit 92 of the cooled CCD camera 81.

The data generation system shown in FIGS. 17 to 20 is produced by contacting a chemiluminescent substrate with a labeling substance contained in a large number of adsorptive membrane regions 4 formed in the biochemical analysis unit 1. Chemiluminescence, camera lens 97
Is detected by the CCD 86 of the cooled CCD camera 81 to generate chemiluminescence data, and the biochemical analysis unit 1 is irradiated with excitation light from the LED light source 100 to be formed in the biochemical analysis unit 1. A fluorescent substance such as a fluorescent dye contained in a large number of the absorptive film regions 4 is excited, and the emitted fluorescent light is passed through the camera lens 97,
The fluorescence data can be generated by detecting with the CCD 66 of the cooling CCD camera 81.

In the case of generating chemiluminescence data, the filter 102 is removed, the LED light source 100 is held in the OFF state, and the biochemical analysis unit 1 is placed on the diffusion plate 103.
The chemiluminescent substrate is brought into contact with the labeling substance contained in the large number of adsorptive film regions 4 formed on the biochemical analysis unit 1 that emits chemiluminescence.

Then, the operator focuses the lens using the camera lens 97 and closes the dark box 82.

After that, when the operator inputs the exposure start signal to the keyboard 85, the exposure start signal changes to the CPU1.
It is input to the camera control circuit 92 of the cooled CCD camera 81 via 10, and the camera control circuit 92 opens the shutter 89 to start the exposure of the CCD 86.

The chemiluminescence emitted from the biochemical analysis unit 1 enters the photoelectric surface of the CCD 86 of the cooled CCD camera 81 through the camera lens 97 and forms an image on the photoelectric surface. The CCD 86 thus receives the light of the image formed on the photocathode and stores it in the form of charges.

Here, in the biochemical analysis unit 1 according to the present embodiment, the absorptive membrane region 4 has the absorptive membrane 2
In addition, the aluminum substrate 5 having a property of attenuating radiation and light and having a large number of through holes formed therein is press-fitted and formed, and a property of attenuating the radiation and light is provided around the adsorptive film region 4. Since the aluminum substrate 5 is provided, the chemiluminescence emitted from the labeling substance contained in the adsorbing film region 4 is chemiluminescence emitted from the labeling substance contained in the adjacent adsorbing film region 4. It can be reliably prevented from being mixed with.

Further, in the present embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is composed of a plurality of nylons 6.
Since many of the plurality of nylon 6 fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 2, the adsorptive membrane 2 is a liquid in a direction parallel to the surface. The permeability of the liquid in the direction perpendicular to the surface is higher than that of the specific binding substance. Therefore, the specific binding substance dropped in the adsorptive membrane region 4 is directed in the direction parallel to the surface of the adsorptive membrane 2. It is possible to effectively prevent it from penetrating and reaching the adjacent adsorbing membrane regions 4, and the specific binding substance dropped in the adsorbing membrane regions 4 can be adsorbed only to the adsorbing membrane regions 4. The specific binding substance dropped onto the adsorbing membrane region 4 permeates into the adsorbing membrane regions 4 adjacent to each other, and the specific binding substance permeating into the adsorbing membrane regions 4 adjacent to each other binds to the radioactive labeling substance and the fluorescent substance. Chemiluminescence by contact with substances and chemiluminescent substrates Results generated causing substance derived from a living organism and labeled with a labeling substance is hybridized, its chemiluminescence to be released from the absorptive membrane region 4, are released from the absorptive membrane region 4 adjacent, cooled CCD camera 81
Therefore, it is possible to surely prevent the photoelectric detection.

In addition, in the present embodiment, the adsorptive film region 4 of the biochemical analysis unit 1 is formed by press-fitting the aluminum substrate 5 in which a large number of through holes are regularly formed into the adsorptive film 2. The voids in the adsorbent film 2 between the adsorbent film regions 4 adjacent to each other have disappeared due to the pressurization, so that the specific binding substance dropped in the adsorbent film region 4 was formed. Penetrates in a direction parallel to the surface of the adsorptive membrane 2 and effectively prevents the adsorbents from reaching the adjacent adsorbent membrane areas 4, and the specific binding substance dropped in the adsorbent membrane areas 4 is prevented. Since it can be adsorbed only to the absorptive membrane region 4, the specific binding substance dropped on the absorptive membrane region 4 permeates the absorptive membrane regions 4 adjacent to each other and the absorptive membrane regions 4 adjacent to each other. Specific binding substances that have penetrated into the As a result of hybridization of a substance of biological origin labeled with a labeling substance that produces chemiluminescence when brought into contact with a chemiluminescent substrate, chemiluminescence to be released from the adsorptive membrane region 4 is adjacent to the adsorbent membrane regions. It is possible to reliably prevent the light from being emitted from No. 4 and photoelectrically detected by the cooled CCD camera 81.

When the predetermined exposure time has elapsed, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 110, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charges to the A / D converter 100, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 11 to operate.
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When an operator inputs a data display signal to the keyboard 85, the CPU 110 causes the data storage means 1 to operate.
The digital data stored in 12 is output to the data processing device 113, data processing is performed in accordance with the instruction of the operator, and then a data display signal is output to the data display means 114 to generate chemiluminescence based on the digital data. The data is displayed on the screen of the CRT 84.

On the other hand, when generating fluorescence data, first, the biochemical analysis unit 1 is placed on the diffusion plate 103.

Then, the LED light source 1 is operated by the operator.
00 is turned on, lens focusing is performed using the camera lens 97, and the dark box 82 is closed.

After that, when the operator inputs an exposure start signal to the keyboard 85, the light source control means 115 turns on the LED light source 100, and the excitation light is emitted toward the biochemical analysis unit 1. At the same time, the exposure start signal is sent to the cooling CCD camera 8 via the CPU 110.
1 to the camera control circuit 92, and the camera control circuit 9
2, the shutter 89 is opened and the exposure of the CCD 86 is started.

The excitation light emitted from the LED light source 100 is filtered by the filter 101 to eliminate wavelength components other than the excitation light, and is made uniform by the diffusion plate 23, and is applied to the biochemical analysis unit 1. It

When the biochemical analysis unit 1 is irradiated with the excitation light, the fluorescent substances contained in the many absorptive film regions 4 formed in the biochemical analysis unit 1 are excited by the excitation light. As a result, fluorescence is emitted.

The fluorescence emitted from the biochemical analysis unit 1 enters the photocathode of the CCD 86 of the cooled CCD camera 81 through the filter 102 and the camera lens 97 and forms an image on the photocathode. CCD86 is
It receives the light of the image formed on the photocathode and stores it in the form of charges. Since the light having the wavelength of the excitation light is cut by the filter 102, only the fluorescence emitted from the fluorescent substance contained in the many absorptive film regions 4 formed in the biochemical analysis unit 1 is absorbed by the CCD 86. Received light.

Here, in the biochemical analysis unit 1 according to this embodiment, the absorptive membrane region 4 comprises the absorptive membrane 2
In addition, the aluminum substrate 5 having a property of attenuating radiation and light and having a large number of through holes formed therein is press-fitted and formed, and a property of attenuating the radiation and light is provided around the adsorptive film region 4. Since the aluminum substrate 5 is provided, the fluorescence emitted from the fluorescent substance contained in the adsorptive film region 4 is mixed with the fluorescence emitted from the fluorescent substance contained in the adjacent adsorptive film region 4. It can be surely prevented from fitting.

Furthermore, in the present embodiment, the adsorptive membrane region 4 of the biochemical analysis unit 1 is composed of a plurality of nylons 6.
Since many of the plurality of nylon 6 fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 2, the adsorptive membrane 2 is a liquid in a direction parallel to the surface. The permeability of the liquid in the direction perpendicular to the surface is higher than that of the specific binding substance. Therefore, the specific binding substance dropped in the adsorptive membrane region 4 is directed in the direction parallel to the surface of the adsorptive membrane 2. It is possible to effectively prevent it from penetrating and reaching the adjacent adsorbing membrane regions 4, and the specific binding substance dropped in the adsorbing membrane regions 4 can be adsorbed only to the adsorbing membrane regions 4. The specific binding substance dropped onto the adsorbing membrane region 4 permeates into the adsorbing membrane regions 4 adjacent to each other, and the specific binding substance permeating into the adsorbing membrane regions 4 adjacent to each other binds to the radioactive labeling substance and the fluorescent substance. Chemiluminescence by contact with substances and chemiluminescent substrates As a result of the substance derived from the living body labeled with the labeling substance being hybridized, the fluorescence that should be emitted from the adsorbing film region 4 is emitted from the adsorbing film region 4 adjacent to the adsorbing film region 4, and the cooled CCD camera 81 photoelectrically converts the fluorescence. It can be reliably prevented from being detected.

In addition, in the present embodiment, the adsorptive film region 4 of the biochemical analysis unit 1 is formed by press-fitting the aluminum substrate 5 in which a large number of through holes are regularly formed into the adsorptive film 2. The voids in the adsorbent film 2 between the adsorbent film regions 4 adjacent to each other have disappeared due to the pressurization, so that the specific binding substance dropped in the adsorbent film region 4 was formed. Penetrates in a direction parallel to the surface of the adsorptive membrane 2 and effectively prevents the adsorbents from reaching the adjacent adsorbent membrane areas 4, and the specific binding substance dropped in the adsorbent membrane areas 4 is prevented. Since it can be adsorbed only to the absorptive membrane region 4, the specific binding substance dropped on the absorptive membrane region 4 permeates the absorptive membrane regions 4 adjacent to each other and the absorptive membrane regions 4 adjacent to each other. Specific binding substances that have penetrated into the As a result of hybridization of a substance derived from a living body labeled with a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate, the fluorescence to be emitted from the adsorptive membrane region 4 is adjacent to the adsorptive membrane region 4 It is possible to reliably prevent the light from being emitted from the camera and being photoelectrically detected by the cooled CCD camera 81.

When the predetermined exposure time elapses, the CPU 11
0 outputs an exposure completion signal to the camera control circuit 92 of the cooled CCD camera 81.

Upon receiving the exposure completion signal from the CPU 40, the camera control circuit 92 transfers the analog data accumulated by the CCD 86 in the form of electric charge to the A / D converter 10, digitizes it, and temporarily stores it in the data buffer 91. To memorize.

At the same time when the exposure completion signal is output to the camera control circuit 92, the CPU 110 causes the data transfer means 21 to operate.
1 outputs a data transfer signal to the cooled CCD camera 81
The digital data is read from the data buffer 91 and stored in the data storage means 112.

When an operator inputs a data display signal to the keyboard 85, the CPU 110 causes the data storage means 1 to operate.
The digital data stored in 12 is output to the data processing device 113, the data processing is performed according to the instruction of the operator, and then the data display signal is output to the data display means 114, and the fluorescence data is output based on the digital data. Is displayed on the screen of the CRT 84.

According to this embodiment, the absorptive membrane region 4 of the biochemical analysis unit 1 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are
Since the adsorbent membranes 2 are arranged in a direction perpendicular to the surface of the adsorbent membrane 2, the adsorbent membrane 2 has a liquid permeability in a direction perpendicular to the surface as compared with a liquid permeability in a direction parallel to the surface. Therefore, it is effective that the specific binding substance dropped in the adsorptive membrane regions 4 permeates in the direction parallel to the surface of the adsorptive membrane 2 and reaches the adjacent adsorbent membrane regions 4. Since it can be prevented, the specific binding substance dropped onto the adsorptive membrane region 4 permeates into the adjacent adsorptive membrane regions 4 and into the specific binding substance permeates into the adjacent adsorptive membrane regions 4, A radio-labeled substance, a fluorescent substance, and a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate are hybridized,
As a result, the electron beam to be emitted from the adsorptive film region 4 is emitted from the adsorbing film regions 4 adjacent to each other, and the stimulable phosphor layer region is arranged to face the adsorbing film regions 4 adjacent to each other. It is possible to surely prevent 12 from being exposed, and the fluorescence and chemiluminescence that should be emitted from the adsorbing film region 4 are emitted from the adsorbing film region 4 adjacent to each other and detected photoelectrically. And the dot-shaped stimulable phosphor delivery region 1 of the stimulable phosphor sheet 10 without fail.
It is possible to detect, with high resolution, the stimulated luminescence 45 emitted from No. 2 and the fluorescence and chemiluminescence emitted from the dot-shaped adsorptive film region 4 of the biochemical analysis unit 1.

Further, according to this embodiment, in the adsorptive film region 4 of the biochemical analysis unit 1, the aluminum substrate 5 having a large number of through holes regularly formed is press-fitted into the adsorptive film 2. The voids in the adsorbent film 2 between the adsorbent film regions 4 adjacent to each other have disappeared due to the pressurization, so that the specific binding substance dropped in the adsorbent film region 4 was formed. Can be effectively prevented from penetrating in the direction parallel to the surface of the adsorptive film 2 and reaching the adsorbent film regions 4 adjacent to each other. The binding substance permeates adjacent adsorbing membrane regions 4, and the specific binding substance permeating adjacent adsorbing membrane regions 4 is brought into contact with a radiolabeling substance, a fluorescent substance, and a chemiluminescent substrate to generate chemiluminescence. A living body labeled with a labeling substance that Substance is hybridized of years,
As a result, the electron beam to be emitted from the adsorptive film region 4 is emitted from the adsorbing film regions 4 adjacent to each other, and the stimulable phosphor layer region is arranged to face the adsorbing film regions 4 adjacent to each other. It is possible to surely prevent 12 from being exposed, and the fluorescence and chemiluminescence that should be emitted from the adsorbing film region 4 are emitted from the adsorbing film region 4 adjacent to each other and detected photoelectrically. And the dot-shaped stimulable phosphor delivery region 1 of the stimulable phosphor sheet 10 without fail.
It is possible to detect, with high resolution, the stimulated luminescence 45 emitted from No. 2 and the fluorescence and chemiluminescence emitted from the dot-shaped adsorptive film region 4 of the biochemical analysis unit 1.

Furthermore, according to the present embodiment, the radiolabeled substance contained in the large number of adsorptive film regions 4 formed in the biochemical analysis unit 1 causes the support 11 of the stimulable phosphor sheet 10 to adhere to the support 11. When exposing the formed dot-shaped stimulable phosphor layer region 12, the large number of the absorptive film regions 4 of the biochemical analysis unit 1 has a property of attenuating radiation in the absorptive film 2. An aluminum substrate 5 having a large number of through holes formed therein is press-fitted and formed, and an aluminum substrate 5 having a property of attenuating radiation is present around the adsorptive film region 4, and further, a stimulable phosphor sheet. Since the support 11 of 10 is made of stainless steel having a property of attenuating radiation, the electron beam emitted from the radiolabel substance contained in the adsorptive film region 4 is reliably prevented from scattering. All the electron beams emitted from the radiolabel substance contained in the adsorptive film region 4 are incident on the stimulable phosphor layer region 12 facing the adsorptive film region 4, and therefore, Biochemical analysis unit 1
In addition, even if the absorptive film regions 4 are formed with high density, it is ensured that they are incident on the stimulable phosphor layer region 12 to be exposed by the electron beam emitted from the adjacent absorptive film regions 4. Therefore, it is possible to effectively prevent generation of noise due to electron beam scattering in the biochemical analysis data generated by photoelectrically detecting the stimulated emission 45. It becomes possible to improve the quantitativeness of biochemical analysis.

Further, according to the present embodiment, a large number of the adsorbing membrane regions 4 of the biochemical analysis unit 1 are arranged on the adsorbing membrane 2.
It has a property of attenuating radiation and light, and is formed by press-fitting an aluminum substrate 5 having a large number of through holes formed therein, and has a property of attenuating radiation and light around each absorptive film region 4. Since the aluminum substrate 5 is present, the fluorescence emitted from the fluorescent substance contained in the large number of adsorbing film regions 4 formed in the biochemical analysis unit 1 is contained in the adsorbing film regions 4 adjacent to each other. It is possible to reliably prevent the generated fluorescent substance from being excited and being mixed with the emitted fluorescent light, and therefore, it is included in a large number of the absorptive film regions 4 formed in the biochemical analysis unit 1. The fluorescent substance is irradiated with excitation light to excite the fluorescent substance, and the fluorescence emitted from the fluorescent substance is photoelectrically detected to generate biochemical analysis data. To be generated Reliably prevented, it is possible to improve the quantification of the biochemical analysis.

Further, according to this embodiment, the absorptive film region 4 of the biochemical analysis unit 1 has a property of attenuating radiation and light in the absorptive film 2, and a large number of through holes are formed. Aluminum substrate 5 is press-fitted and formed,
Since the aluminum substrate 5 having the property of attenuating radiation and light is present around the adsorptive film region 4, it is released from the large number of adsorptive film regions 4 formed in the biochemical analysis unit 1. It is possible to reliably prevent the chemiluminescence from mixing with the chemiluminescence emitted from the adsorbent membrane regions 4 adjacent to each other, and therefore, from the large number of adsorbent membrane regions 4 formed in the biochemical analysis unit 1. It is possible to reliably prevent the generation of noise due to the scattering of chemiluminescence in the data for biochemical analysis generated by photoelectrically detecting the emitted chemiluminescence to improve the quantitativeness of biochemical analysis. It will be possible to improve.

Further, according to this embodiment, in the adsorptive membrane region 4 of the biochemical analysis unit 1, a large number of through holes are regularly formed in the adsorptive membrane 2 formed by a plurality of nylon 6 fibers. Since the formed aluminum substrate 5 is formed by press-fitting, the voids in the adsorptive film 2 between the adjacent adsorbent film regions 4 are, when the aluminum substrate 5 is press-fitted,
It disappears by pressurization, and therefore, the specific binding substance dropped in the adsorptive film region 4 is effectively prevented from diffusing into the region of the adsorptive film 2 other than the adsorptive film region 4, The specific binding substance dropped in the adsorptive membrane region 4 is
Since it is adsorbed only to the adsorptive membrane region 4, it becomes possible to greatly improve the quantitativeness of the biochemical analysis.

Furthermore, according to this embodiment, the biochemical analysis unit 1 is formed by press-fitting the aluminum substrate 5 into the absorptive film 2 formed of a plurality of nylon 6 fibers. Even if it is subjected to a treatment with a liquid such as hybridization, it hardly expands or contracts, and a large number of the absorptive film regions 4 formed in the biochemical analysis unit 1 are supported by the support 11 of the stimulable phosphor sheet 10.
A large number of dot-shaped stimulable phosphor layer regions 12 formed on
Then, the stimulable phosphor sheet 10 and the biochemical analysis unit 1 can be easily and surely overlapped with each other so as to face each other accurately, and the dot-shaped stimulable phosphor layer region 12 can be exposed. become.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.
2 is a partial sectional view thereof.

As shown in FIGS. 21 and 22, the biochemical analysis unit 121 according to this embodiment is similar to that shown in FIG.
And similar to the embodiment shown in FIG. 2, it comprises an adsorbent membrane 122 formed from a number of nylon 6 fibers.

Also in this embodiment, the absorptive film 122.
In the same manner as in the above embodiment, a plurality of nylon 6 fibers are bundled, and a part of the bundle is impregnated with an adhesive to generate a fiber bundle of nylon 6 fibers. It is formed by slicing along the direction orthogonal to. Therefore, most of the nylon 6 fibers are arranged in a direction perpendicular to the surface of the adsorptive membrane 122.

As shown in FIGS. 21 and 22, metal oxide particles were dispersed in plastic to be formed on the adsorptive film 122, and a large number of substantially circular through holes were regularly formed. The plastic substrate 125 is press-fitted by the calendering device shown in FIG. 4, so that a plurality of the adsorbent film regions 124 are arranged in a dot shape corresponding to the plurality of through holes formed in the plastic substrate 125. Has been formed. Here, the metal oxide particles,
The plastic substrate 125 formed by being dispersed in plastic has a property of attenuating radiation and light, and the adhesive 12 is formed on the back surface of the plastic substrate 125.
3 is applied, and the plastic substrate 125 is press-fitted into the adsorptive film 122 via the adhesive 123. Therefore, the plastic substrate 125 includes the adsorptive film 122,
It is firmly integrated to improve durability.

Although not shown exactly in FIG. 21, in the present embodiment, the generally circular adsorbent membrane region 12 has a size of about 0.01 square millimeters of about 10,000.
4 are regularly formed in the biochemical analysis unit 121 with a density of about 5000 pieces / square centimeter.

As shown in FIG. 22, in the present embodiment, the plastic substrate 125 is arranged so that the surface of the plastic substrate 125 is located above the surface of each of the absorptive film regions 124. The biochemical analysis unit 121 is formed by press fitting.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
Similarly to the above, a large number of absorptive membrane regions 1 formed in the biochemical analysis unit 121 by the spotting device 1
A specific binding substance such as cDNA is dropped onto 24,
Adsorbed.

Also in this embodiment, the adsorptive membrane region 124 of the biochemical analysis unit 121 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are adsorbent. Since the adsorptive membrane 122 is arranged in the direction perpendicular to the surface of the membrane 122, the adsorptive membrane 122 has a higher permeability of the liquid in the direction perpendicular to the surface than the permeability of the liquid in the direction parallel to the surface. Therefore, it is possible to effectively prevent the specific binding substance dropped in the adsorptive film regions 124 from penetrating in the direction parallel to the surface of the adsorptive film 122 and reaching the adjacent adsorptive film regions 124. be able to.

Further, also in the present embodiment, in the adsorptive film region 124 of the biochemical analysis unit 121, the plastic substrate 125 having a number of through holes regularly formed is press-fitted into the adsorptive film 122, Since it is formed, the voids in the adsorptive film 122 between the adjacent adsorptive film regions 124 disappear due to the pressurization, and therefore, the specific binding substance dropped in the adsorptive film regions 124 is , Is effectively prevented from penetrating in the direction parallel to the surface of the adsorptive film 122 and reaching the adsorbent film regions 124 adjacent to each other, and the specific binding substance dropped in the adsorptive film region 124 is adsorbed. It can be adsorbed only to the film region 124.

Further, as shown in FIG. 6, chemiluminescence is obtained by contacting a biogenic substance labeled with a radioactive labeling substance, a biogenic substance labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. Hybridization container 8 containing a hybridization liquid 9 containing a substance of biological origin labeled with a labeling substance that causes
A biochemical analysis unit 121 is set inside, and a specific binding substance such as cDNA adsorbed on a large number of adsorptive membrane regions 124 is labeled with a radioactive labeling substance and is derived from a living body contained in the hybridization liquid 9. Of the biogenic substance contained in the hybridizing liquid 9 and the biogenic substance contained in the hybridizing liquid 9 and the biogenic substance contained in the hybridizing liquid 9 , Selectively, hybridize.

Also in the present embodiment, the adsorptive membrane region 124 of the biochemical analysis unit 121 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are adsorbent. Since the adsorptive membrane 122 is arranged in the direction perpendicular to the surface of the membrane 122, the adsorptive membrane 122 has a higher permeability of the liquid in the direction perpendicular to the surface than the permeability of the liquid in the direction parallel to the surface. Therefore, the specific binding substance dropped in the adsorptive membrane regions 124 is effectively prevented from penetrating in the direction parallel to the surface of the adsorptive membranes 122 and reaching the adjacent adsorbent membrane regions 124. The specific binding substance dropped in the adsorptive film region 124 can be adsorbed only to the adsorptive film region 124.
The specific binding substance dropped onto the adsorptive membrane region 124 permeates into the adjacent adsorptive membrane regions 124, and the specific binding substance permeates into the adjacent adsorptive membrane regions 124 is labeled with a radiolabel, a fluorescent substance and It is possible to reliably prevent the substance derived from the living body labeled with the labeling substance that causes chemiluminescence by contacting with the chemiluminescent substrate from hybridizing.

Also in this embodiment, the absorptive membrane region 124 of the biochemical analysis unit 121 has the absorptive membrane 1
Since the plastic substrate 125 in which a large number of through holes are regularly formed is press-fitted into the 22, the voids in the adsorbent film 122 in the adsorbent film regions 124 adjacent to each other disappear by the pressurization. And therefore the adsorptive membrane area 1
The specific binding substance dropped in 24 is absorbed by the adsorptive film 122.
Permeating in a direction parallel to the surface of the adsorbent film region 1 and effectively preventing it from reaching the adjacent adsorbent film region 124.
Since the specific binding substance dropped in 24 can be adsorbed only to the adsorptive membrane region 124, the specific binding substance dropped to the adsorptive membrane region 124 is adjacent to the adsorptive membrane region 124. Specific binding substance that has penetrated into the adsorbent membrane region 124 adjacent to the
It becomes possible to reliably prevent the substance of biological origin labeled with the labeling substance that causes chemiluminescence by contacting with the fluorescent substance and the chemiluminescent substrate from hybridizing.

Thus, the biochemical analysis unit 121
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

Fluorescence data recorded in the biochemical analysis unit 121 is recorded in the scanner shown in FIGS. 9 to 16 or FIGS.
The cooled CCD camera 81 of the data generation system shown in FIG.
By the above, the biochemical analysis data is read and the chemiluminescence data recorded in the biochemical analysis unit 121 is the data shown in FIGS. 17 to 20 in the same manner as in the above embodiment. Cooling CCD for production system
The data is read by the camera 81 and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 12
The radiation data of the radiolabeled substance recorded in 1 is transferred to the stimulable phosphor sheet.

FIG. 23 is a schematic perspective view of the stimulable phosphor sheet 130.

As shown in FIG. 23, the stimulable phosphor sheet 130 has a support 131, and one surface of the support 131 has a large number of adsorptive films formed on the biochemical analysis unit 121. A large number of substantially circular dot-shaped stimulable phosphor layer regions 132 are formed in the same regular pattern as the region 124.

Also in this embodiment, the support 131 is
A stimulable phosphor layer region 132 formed of stainless steel having a property of attenuating radiation and having a substantially circular dot shape.
Are formed on the surface of the support 121 so as to have the same diameter as the adsorptive membrane region 124 formed in the biochemical analysis unit 121.

FIG. 24 shows a large number of dot-shaped stimulants formed on the stimulable phosphor sheet 130 by the radioactive labeling substance contained in the large number of absorptive film regions 124 formed in the biochemical analysis unit 121. FIG. 6 is a schematic cross-sectional view showing a method of exposing the phosphor layer region 132.

As shown in FIG. 24, upon exposure, a large number of absorptive film regions 124 formed in the biochemical analysis unit 121 were formed on the surface of the support 131 of the stimulable phosphor sheet 130. The stimulable phosphor sheet 130 and the biochemical analysis unit 121 are superposed so as to face the large number of dot-shaped stimulable phosphor layer regions 132.

Also in this embodiment, the biochemical analysis unit 121 has a plastic substrate 12 in which metal oxide particles are dispersed in plastic on an adsorptive film 122 formed of nylon 6 capable of forming a membrane filter.
Since 5 is pressed and formed, it hardly expands or contracts even when subjected to a treatment with a liquid such as hybridization. Therefore, a large number of absorptive membrane regions 124 formed in the biochemical analysis unit 121 are formed. Of the stimulable phosphor sheet 130 and the biochemistry so as to exactly face the large number of dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of the stimulable phosphor sheet 130. The dot-shaped stimulable phosphor layer region 132 can be exposed by easily and surely overlapping the analysis unit 121.

In this way, each of a large number of dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of the stimulable phosphor sheet 130 and the biochemical analysis unit 121 for a predetermined time. By facing the large number of absorptive film regions 124 formed on the surface of the support 131 of the stimulable phosphor sheet 130, a large number of the absorptive phosphor regions 130 are formed by the radioactive labeling substance contained in the absorptive film regions 124. The dot-shaped stimulable phosphor layer region 132 is exposed.

At this time, an electron beam is emitted from the radioactive labeling substance adsorbed on the adsorptive film region 124, but the adsorptive film region 124 of the biochemical analysis unit 121 penetrates the adsorptive film 122 a number of times. Since the plastic substrate 125 having the property of attenuating the radiation is press-fitted and formed, and the plastic substrate 125 having the property of attenuating the radiation is present around the adsorptive film region 124. Electron beams emitted from the radiolabeled substance contained in the absorptive film region 124 are reliably prevented from scattering, and all electron beams emitted from the radiolabeled substance contained in the absorptive film region 124 are , Is exposed to the electron beam incident on the photostimulable phosphor layer region 132 facing the adsorptive film region 124 and emitted from the adjacent adsorptive film region 124. It is possible to reliably prevent incident on the stimulable phosphor layer regions 132 to.

Therefore, a large number of dot-shaped stimulable phosphor layer regions 132 formed on the stimulable phosphor sheet 130.
Can be reliably exposed only by the radioactive labeling substance contained in the corresponding adsorptive film region 124 of the biochemical analysis unit 121.

Also in this embodiment, the absorptive membrane region 124 of the biochemical analysis unit 121 is formed of a bundle of a plurality of nylon 6 fibers, and a plurality of nylon 6
Most of the fibers of the adsorbent membrane 122 are arranged in a direction perpendicular to the surface of the adsorptive membrane 122, so that the adsorbent membrane 122 is perpendicular to the surface in comparison with the liquid permeability in the direction parallel to the surface. The liquid has a high permeability in the direction, and therefore the specific binding substance dropped in the adsorptive membrane regions 124 permeates in the direction parallel to the surface of the adsorptive membrane 122 to the adjacent adsorptive membrane regions 124. Since it can be effectively prevented from reaching, and the specific binding substance dropped in the adsorptive film region 124 can be adsorbed only to the adsorptive film region 124,
The specific binding substance dropped onto the adsorptive membrane region 4 permeates into the adjacent adsorptive membrane regions 4, and the substance of biological origin labeled with the radiolabel substance is hybridized. Electron beam to be emitted from
The stimulable phosphor layer region 12 which is emitted from the adsorbing film regions 4 adjacent to each other and is arranged to face the adsorbing film regions 4 adjacent to each other.
It is possible to reliably prevent the exposure of.

Further, also in this embodiment, the absorptive film region 124 of the biochemical analysis unit 121 press-fits the plastic substrate 125 in which a large number of through holes are regularly formed in the absorptive film 122, Since it is formed, the voids in the adsorptive film 122 between the adjacent adsorptive film regions 124 disappear due to the pressurization, and therefore, the specific binding substance dropped in the adsorptive film regions 124 is Effectively preventing the permeation into the region of the adsorptive film 122 other than the adsorptive film region 124, the specific binding substance dropped in the adsorptive film region 124 is only applied to the adsorptive film region 124. Since they can be adsorbed, the specific binding substance dropped onto the adsorbent film region 124 is adjacent to the adsorbent film region 1.
As a result of permeation into 24 and hybridization of a substance derived from a living body labeled with a radioactive labeling substance, an electron beam to be emitted from the adsorptive membrane region 124 is emitted from the adjacent adsorptive membrane regions 124 and is adjacent to each other. Adsorbent membrane area 12
It is possible to reliably prevent exposure of the stimulable phosphor layer region 132 arranged so as to face No. 4.

Thus, a large number of dot-shaped stimulable phosphor layer regions 132 formed on the stimulable phosphor sheet 130 are
The radiation data of the radio-labeled substance is recorded, and the radiation data recorded in a large number of dot-shaped stimulable phosphor layer regions 132 is the same as in the above embodiment, and the scanner shown in FIGS. 9 to 16 is used. Is read out and biochemical analysis data is generated.

According to this embodiment, the adsorptive membrane region 124 of the biochemical analysis unit 121 is formed by a bundle of a plurality of nylon 6 fibers, and most of the plurality of nylon 6 fibers are absorptive membrane 122. Since it is arranged in the direction perpendicular to the surface of the liquid, the adsorptive membrane 122 has a higher liquid permeability in the direction perpendicular to the surface than the liquid permeability in the direction parallel to the surface. , Adsorptive membrane area 124
The specific binding substance dropped inside penetrates in the direction parallel to the surface of the adsorptive membrane 122, and the adsorbent membrane regions 12 adjacent to each other.
You can effectively prevent reaching 4.
The specific binding substance dropped onto the adsorptive membrane region 124 permeates into the adjacent adsorptive membrane regions 124, and the specific binding substance permeates into the adjacent adsorptive membrane regions 124 is labeled with a radiolabel, a fluorescent substance and A substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is hybridized, and as a result,
The electron beam to be emitted from the adsorptive film region 124 is
It is possible to reliably prevent exposure of the stimulable phosphor layer region 132, which is emitted from the adsorbing film regions 124 adjacent to each other and is arranged to face the adsorbing film regions 124 adjacent to each other, and Fluorescence and chemiluminescence that should be emitted from the adsorbent film region 124 are adjacent to each other.
The photostimulable light 45 emitted from the dot-shaped stimulable phosphor delivery region 132 of the stimulable phosphor sheet 130 and the biochemical analysis by effectively preventing it from being emitted from 24 and photoelectrically detected. Fluorescence and chemiluminescence emitted from the dot-shaped adsorptive film region 124 of the unit 121 can be detected with high resolution.

Further, according to this embodiment, the absorptive membrane region 124 of the biochemical analysis unit 121 is the absorptive membrane 12
Since the plastic substrate 125 in which a large number of through holes are regularly formed is press-fitted in 2, the voids in the adsorptive film 122 between the adjacent adsorptive film regions 124 are:
The specific binding substance dropped in the adsorptive film region 124 due to the disappearance due to the pressurization is thus absorbed.
2 can be effectively prevented from penetrating in the direction parallel to the surface of No. 2 and reaching the adsorbing membrane regions 124 adjacent to each other. A specific binding substance that permeates the adsorbing membrane regions 124 that match each other and that permeates the adsorbing membrane regions 124 that adjoin each other is labeled with a labeling substance that causes chemiluminescence by contacting with a radioactive labeling substance, a fluorescent substance, and a chemiluminescent substrate. As a result, the substance derived from the living body is hybridized, and as a result, the electron beam to be emitted from the adsorptive membrane region 124 is emitted from the adjacent adsorptive membrane regions 124 and faces the adjacent adsorptive membrane regions 124. It is possible to reliably prevent the photostimulable phosphor layer region 132 that is arranged as a whole from being exposed to light, and at the same time, it is possible to prevent the fluorescence and the fluorescence that should be emitted from the adsorptive film region 124. And chemiluminescence are surely prevented from being emitted from the adsorbing film regions 124 adjacent to each other and photoelectrically detected, and are emitted from the dot-shaped stimulable phosphor delivery region 132 of the stimulable phosphor sheet 130. It is possible to detect the stimulated emission 45 and the fluorescence and chemiluminescence emitted from the dot-shaped adsorptive film region 4 of the biochemical analysis unit 1 with high resolution.

Furthermore, the dots formed on the surface of the support 131 of the stimulable phosphor sheet 130 by the radioactive labeling substance contained in the many absorptive film regions 124 formed in the biochemical analysis unit 121. The biochemical analysis unit 12 when exposing the photostimulable phosphor layer region 132 in the shape of a circle.
The large number of the adsorbent film regions 124 in one is the adsorbent film 122.
A plastic substrate 125 having a property of attenuating radiation and having a large number of through holes is press-fitted to be formed,
A plastic substrate 125 having a property of attenuating radiation exists around the adsorptive film region 124, and further,
Since the support 131 of the stimulable phosphor sheet 130 is formed of stainless steel having a property of attenuating radiation, the electron beam emitted from the radioactive labeling substance contained in the adsorptive film region 124 is scattered. The electron beam emitted from the radio-labeled substance contained in the adsorptive film region 124 can all be prevented, and the stimulable phosphor layer region 132 facing the adsorptive film region 124 can be used.
Incident on the biochemical analysis unit 121.
In addition, even if the absorptive film regions 124 are formed at a high density, it is ensured that they are incident on the stimulable phosphor layer region 132 to be exposed by the electron beam emitted from the adjacent absorptive film regions 124. Therefore, it is possible to effectively prevent generation of noise due to electron beam scattering in the biochemical analysis data generated by photoelectrically detecting stimulated emission. It becomes possible to improve the quantitativeness of chemical analysis.

Further, according to the present embodiment, a large number of the absorptive film regions 124 of the biochemical analysis unit 121 have a property that the absorptive film 122 attenuates radiation and light.
A plastic substrate 125 having a large number of through holes is press-fitted to be formed, and the plastic substrate 125 having a property of attenuating radiation and light is present around each adsorptive film region 124. The fluorescence emitted from the fluorescent substance contained in the large number of adsorptive film regions 124 formed in the chemical analysis unit 121 is emitted by exciting the fluorescent substance contained in the adjacent adsorptive film regions 124. It is possible to reliably prevent the fluorescent substance from being mixed with the fluorescent substance. Therefore, by irradiating the fluorescent substance contained in the many absorptive film regions 124 formed in the biochemical analysis unit 121 with the excitation light, Ensures that noise caused by fluorescence scattering is generated in the data for biochemical analysis generated by exciting the fluorescent substance and photoelectrically detecting the fluorescence emitted from the fluorescent substance. Prevention to, it is possible to improve the quantification of the biochemical analysis.

Furthermore, according to the present embodiment, the large number of absorptive film regions 124 of the biochemical analysis unit 121 have a property of attenuating radiation and light in the absorptive film 122 and have a large number of through holes. Formed plastic substrate 125
Since a plastic substrate 125 having a property of attenuating radiation and light is present around each of the absorptive film regions 124 formed by being pressed in, a large number of the biochemical analysis unit 121 formed. The chemiluminescence emitted from the adsorbent film regions 124 is generated by the adjacent adsorbent film regions 124.
It is possible to reliably prevent mixing with the chemiluminescence emitted from the biochemical analysis unit 1.
In the biochemical analysis data generated by photoelectrically detecting chemiluminescence emitted from the large number of adsorptive film regions 124 formed in 21, noise generated due to chemiluminescence scattering may be generated. It is possible to reliably prevent and improve the quantitativeness of biochemical analysis.

Further, according to the present embodiment, the large number of adsorptive film regions 124 of the biochemical analysis unit 121 are formed by a plurality of nylon 6 fibers.
Since the plastic substrate 125 in which a large number of through holes are regularly formed is press-fitted to be formed, the gap in the adsorptive film 122 between the adjacent adsorptive film regions 124 is formed by the plastic substrate 125. When press-fitting, it disappears due to pressurization, so that the specific binding substance dropped in the adsorptive film region 124 is effective in diffusing into the region of the adsorptive film 122 other than the adsorptive film region 124. The specific binding substance that has been prevented from occurring and is dropped into the adsorptive film region 124 is adsorbed only to the adsorptive film region 124, so that it becomes possible to greatly improve the quantitativeness of the biochemical analysis.

Further, according to this embodiment, the biochemical analysis unit 121 includes the plastic substrate 125 on the absorptive film 122 formed of a plurality of nylon 6 fibers.
Since it is press-fitted and formed, even if it is subjected to a treatment with a liquid such as hybridization, it hardly expands or contracts, and a large number of absorptive membrane regions 124 formed in the biochemical analysis unit 1 accumulate. Fluorescent phosphor sheet 13
The stimulable phosphor sheet 130 and the biochemical analysis unit 121 so as to accurately face the large number of dot-shaped stimulable phosphor layer regions 132 formed on the surface of the support 131 of 0. The dot-shaped stimulable phosphor layer regions 132 can be exposed with ease and certainty.

FIG. 25 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 25, the biochemical analysis unit 141 according to the present embodiment includes an absorptive substrate 142 made of nylon 6.

In this embodiment, the absorptive substrate 142 is used.
Is obtained by bundling a plurality of nylon 6 hollow fibers, fusing a part thereof to produce a nylon 6 fiber bundle, and slicing along a direction orthogonal to the axis of the nylon 6 fiber bundle, Has been formed.

FIG. 26 is a process chart showing an example of a method for producing the absorptive substrate 142.

As shown in FIG. 3A, an adhesive, for example, a hot-melt adhesive 142b is impregnated into an appropriate portion of the surface of each hollow fiber 142a of nylon 6.

Next, as shown in FIG. 3B, the nylon 6 hollow fiber bundles produced by bundling a large number of nylon 6 hollow fibers 142a are aligned with the axis of many nylon 6 hollow fibers 142a. A compressed body 142c of nylon 6 having a rectangular cross section is formed by being pressed from orthogonal directions and made of a large number of hollow fibers of nylon 6.

As shown in FIG. 3 (C), the compressed body 142c thus produced is sliced to a predetermined thickness along the plane orthogonal to the axis of many hollow fibers 142a of nylon 6 and the adsorptivity is improved. The substrate 1422 is produced.

Therefore, most of the hollow fibers of nylon 6 are arranged in the direction perpendicular to the surface of the adsorptive substrate 142.

Also in this embodiment, the biochemical analysis unit 1 according to the embodiment shown in FIGS. 1 and 2 is used.
Similarly, a specific binding substance such as cDNA is dropped onto a predetermined region of the surface of the adsorptive substrate 142 of the biochemical analysis unit 141 by a spotting device,
Adsorbed.

In this embodiment, the absorptive substrate 142 of the biochemical analysis unit 141 is formed by a bundle of a plurality of nylon 6 hollow fibers, and most of the plurality of nylon 6 hollow fibers are absorptive substrate 142. Since the adsorptive substrate 142 is arranged in the direction perpendicular to the surface of the liquid, the adsorptive substrate 142 has higher liquid permeability in the direction perpendicular to the surface as compared with the liquid permeability in the direction parallel to the surface. Since it is possible to effectively prevent the specific binding substance dropped in the adsorptive substrate 142 from penetrating in the direction parallel to the surface of the adsorptive substrate 142, the specific binding substance is dropped and adsorbed. It becomes possible to form the spot regions on the flexible substrate 142 with high density.

Further, as shown in FIG. 6, chemiluminescence is obtained by contacting a biogenic substance labeled with a radioactive labeling substance, a biogenic substance labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. Hybridization container 8 containing a hybridization liquid 9 containing a substance of biological origin labeled with a labeling substance that causes
A biochemical analysis unit 121 is set therein, and a specific binding substance such as cDNA adsorbed on the absorptive substrate 142 is labeled with a radioactive labeling substance and is a substance of biological origin contained in the hybridizing solution 9; A biological substance contained in the hybridizing liquid 9 and labeled with a fluorescent substance such as a fluorescent dye and a biological substance contained in the hybridizing liquid 9 are selectively selected. To hybridize.

Thus, the biochemical analysis unit 141
The radiation data, the fluorescence data and the chemiluminescence data are recorded in.

The fluorescence data recorded in the biochemical analysis unit 141 is the same as in the above embodiment, and the scanner shown in FIGS. 9 to 16 or FIGS.
The cooled CCD camera 81 of the data generation system shown in FIG.
By the above, the biochemical analysis data is generated and the chemiluminescence data recorded in the biochemical analysis unit 141 is the data shown in FIG. 17 to FIG. Cooling CCD for production system
The data is read by the camera 81 and biochemical analysis data is generated.

On the other hand, the biochemical analysis unit 14
The radiation data of the radio-labeled substance recorded in No. 1 was transferred to a stimulable phosphor sheet and read by the scanner shown in FIGS. 9 to 16 in the same manner as in the above-mentioned embodiment to be used for biochemical analysis. Data is generated.

According to this embodiment, the absorptive substrate 142 of the biochemical analysis unit 121 is formed by a bundle of a plurality of nylon 6 hollow fibers, and most of the plurality of nylon 6 hollow fibers are absorptive substrates. Since the adsorptive substrate 142 is arranged in the direction perpendicular to the surface of the 142, the adsorptive substrate 142 has higher liquid permeability in the direction perpendicular to the surface as compared with the liquid permeability in the direction parallel to the surface. The specific binding substance dropped on the adsorptive substrate 142 is
Since it can be effectively prevented from penetrating in the direction parallel to the surface of the second substrate 2, even if the spot region of the specific binding substance is formed on the surface of the absorptive substrate 142 at a high density, the specific binding substance The substance is hybridized with a substance of biological origin that is labeled with a radioactive labeling substance and selectively labeled with the radioactive labeling substance, and the photostimulable phosphor layer exposed by the radioactive labeling substance contained in the spot area. In addition, it is possible to irradiate laser light to the photostimulable light emitted from the photostimulable phosphor layer with high resolution and photoelectrically detect it, and generate biochemical analysis data with excellent quantification. become.

Further, according to this embodiment, the absorptive substrate 142 of the biochemical analysis unit 121 is formed of a bundle of a plurality of nylon 6 hollow fibers, and a plurality of nylon 6
Since many of the hollow fibers of the adsorbent substrate 142 are arranged in the direction perpendicular to the surface of the adsorbent substrate 142, the adsorbent substrate 142 is perpendicular to the surface of the adsorbent substrate 142 as compared with the liquid permeability in the direction parallel to the surface. Since the liquid has high permeability in the direction, and therefore the specific binding substance dropped onto the adsorptive substrate 142 can be effectively prevented from penetrating in the direction parallel to the surface of the adsorptive substrate 142, Even if the spot region of the specific binding substance is formed on the surface of the absorptive substrate 142 at a high density, by contacting the specific binding substance with the substance derived from the living body labeled with the fluorescent substance and the chemiluminescent substrate. Biochemical analysis unit 14 in which a large number of spot regions are selectively labeled by hybridizing a substance of biological origin labeled with a labeling substance that causes chemiluminescence
1, irradiating the excitation hole to excite the fluorescent substance and generate chemiluminescence by contacting the fluorescence emitted from the fluorescent substance and the chemiluminescent substrate with high resolution. It becomes possible to generate data for biochemical analysis which is detected photoelectrically and has excellent quantitativeness.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above-described embodiment, a plurality of cDNAs having different base sequences and different from each other are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to the cDNA. Hormones, tumor markers, enzymes, antibodies, antigens,
Abzyme, other proteins, nucleic acids, cDNA, D
All specific binding substances, such as NA and RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. are known, should be used as the specific binding substance of the present invention. You can

Furthermore, in the embodiment shown in FIGS. 1 and 2 and the embodiment shown in 21 and FIG. 22, the adsorptive membrane 2 of the biochemical analysis unit 1, 121,
122 is formed by a plurality of nylon 6 fibers,
In the embodiment shown in FIG. 25, the absorptive substrate 142 of the biochemical analysis unit 141 is composed of a plurality of nylon 6
However, it is not always necessary to form the absorptive membranes 2 and 122 of the biochemical analysis units 1 and 121 and the absorptive substrate 142 of the biochemical analysis unit 141 from nylon 6 fibers. Not. The absorptive membranes 2, 122 of the biochemical analysis unit 1, 121 and the absorptive substrate 142 of the biochemical analysis unit 141 are preferably formed of a fiber material or a porous material,
Although it is particularly preferable to use a fibrous material, a fibrous material and a porous material can be used together to form the absorptive region. Biochemical analysis unit 1,121
The fibrous material used to form the absorptive membranes 2 and 122 of FIG. 2 and the absorptive substrate 142 of the biochemical analysis unit 141 is not particularly limited, but preferably, for example, nylon 6 or nylon 6 is used. , 6, nylon 4, 1
Examples thereof include nylons such as 0, cellulose derivatives such as nitrocellulose, cellulose acetate, and cellulose acetate butyrate. The porous material used to form the adsorptive films 2 and 122 of the biochemical analysis units 1 and 121 and the adsorptive substrate 142 of the biochemical analysis unit 141 may be either an organic material or an inorganic material. Well, it may be an organic / inorganic composite. The organic porous material used to form the adsorptive films 2 and 122 of the biochemical analysis units 1 and 121 and the adsorptive substrate 142 of the biochemical analysis unit 141 is not particularly limited. A carbon material such as activated carbon or a porous material capable of forming a membrane filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6 and nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid / polylysine polyion complex, etc. Examples thereof include alginic acids; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluorides such as polytetrafluoride, and copolymers or composites thereof. The inorganic porous material used to form the adsorptive films 2 and 122 of the biochemical analysis units 1 and 121 and the adsorptive substrate 142 of the biochemical analysis unit 141 is not particularly limited. Preferably, for example, platinum, gold,
Metals such as iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite;
Examples thereof include metal salts such as hydroxyapatite and calcium sulfate, and composites thereof.

In the embodiment shown in FIGS. 1 and 2, the biochemical analysis unit 1 is produced by press-fitting the aluminum substrate 5 into the adsorptive film 2 using a calender roll. In the embodiment shown in FIGS. 21 and 22, the biochemical analysis unit 121 is a plastic substrate 125 obtained by dispersing metal oxide particles in plastic on the adsorptive film 122 using a calendar roll. The aluminum substrate 5 or the plastic substrate 125 is not necessarily pressed into the absorptive films 2 and 122 using a calender roll, but other press-fitting means such as a hot press is used. Alternatively, the aluminum substrate 5 or the plastic substrate 125 may be press-fitted into the absorptive films 2 and 122. Te, by a suitable method, the aluminum substrate 5 or a plastic substrate 125, embedded in absorptive membrane 2,122, biochemical analysis unit 1,
121 may be formed.

Further, in the embodiment shown in FIGS. 1 and 2, the aluminum substrate 5 is press-fitted into the adsorptive film 2 to produce the biochemical analysis unit 1, which is shown in FIGS. 21 and 22. In the disclosed embodiment, the adsorptive membrane 12
The plastic substrate 125 obtained by dispersing the metal oxide particles in the plastic is press-fitted in 2 to generate the biochemical analysis unit 121. The metal oxide particles are dispersed in the aluminum 5 or the plastic. It is not always necessary to use the obtained plastic substrate 125, and the material is not particularly limited as long as it is a plate-shaped member formed of a material having a property of attenuating radiation and light and having a plurality of through holes formed therein. However, either an inorganic compound material or an organic compound material can be used, but a metal material, a ceramic material or a plastic material is particularly preferably used. Examples of the inorganic compound material capable of attenuating radiation and / or light which can be preferably used for forming the plate member include, for example, gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron and nickel. , Cobalt, lead,
Metals such as tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; tungsten. Inorganic salts such as carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, as the organic compound material capable of attenuating radiation and / or light, a polymer compound is preferably used, and the radiation and / or light preferably usable for forming the plate-shaped member can be attenuated. Examples of the polymer compound include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoro. Ethylene; polychlorotrifluoroethylene; polycarbonate; polyesters such as polyethylene naphthalate and polyethylene terephthalate; nylon 6,
Nylon such as nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenylsiloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer;
Polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan; sumac;
Examples thereof include polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds. These may be composite materials, if necessary, it is possible to fill with metal oxide particles or glass fibers,
Also, an organic compound material can be blended and used.

In the embodiment shown in FIGS. 1 and 2, the aluminum substrate 5 is press-fitted into the adsorptive film 2 via the adhesive 3 to form the biochemical analysis unit 1. 21 and FIG. 22, a plastic substrate 125 made by dispersing metal oxide particles in plastic is press-fitted into the adsorptive film 122 via an adhesive 123 for biochemical analysis. Unit 121
However, it is not always necessary to use the adhesives 3, 123.

Further, in the embodiment shown in FIGS. 1 and 2, the aluminum substrate 5 is press-fitted into the adsorptive film 2 so that the aluminum substrate 5 exists around each adsorptive film region 4. In the embodiment shown in FIGS. 21 and 22, the biochemical analysis unit 1 is produced, and the plastic substrate 125 produced by dispersing the metal oxide particles in the plastic is pressed into the adsorptive film 122. Then, the biochemical analysis unit 121 is arranged so that the plastic substrate 125 exists around each adsorptive film region 124.
However, the stimulable phosphor sheets 10 and 130 are generated.
When only the radiation data recorded in the large number of dot-shaped stimulable phosphor layer regions 12 and 132 are detected to generate the biochemical analysis data, the absorptive film regions 4 and 124 are formed.
A region having a property of transmitting light but attenuating radiation may be formed in the periphery of the, while meanwhile,
When the biochemical analysis data is generated by detecting only the fluorescence data or the chemiluminescence data recorded in the many adsorptive film regions 4 and 124 of the biochemical analysis units 1 and 121, each adsorptive film is used. A region that transmits radiation but attenuates light may be formed around the regions 4 and 124.

Further, in the embodiment shown in FIGS. 1 and 2 and the embodiments shown in FIGS. 21 and 22, a generally circular adsorptive membrane having a size of about 0.01 square millimeters of about 10,000. The regions 4, 124 have a density of about 5000 pieces / square centimeter, and follow the regular pattern according to the biochemical analysis units 1, 121.
However, the absorptive membrane regions 4 and 124 are not necessarily formed in a substantially circular shape, and the absorptive membrane regions 4 and 124 may be formed in an arbitrary shape, for example, a rectangular shape. it can.

Also, in the embodiment shown in FIGS. 1 and 2 and in the embodiments shown in FIGS. 21 and 22, a substantially circular adsorptive membrane having a size of about 0.01 square millimeters of about 10,000. Area 4,124 is about 5
The biochemical analysis units 1 and 121 are formed in a regular pattern with a density of 000 pieces / square centimeter, but the number and size of the adsorptive membrane regions 4 and 124 are arbitrary depending on the purpose. A choice can be made, preferably 50 or more adsorbent membrane regions 4,124.
Is formed in the biochemical analysis unit 1, 121 with a density of 50 pieces / cm 2 or more, and the adsorptive membrane regions 4, 124 are formed in the biochemical analysis unit 1, 121 with a size of less than 5 mm 2. To be done.

Further, in the embodiment shown in FIGS. 1 and 2 and the embodiments shown in FIGS. 21 and 22, a generally circular adsorptive membrane having a size of about 0.01 square millimeters of about 10,000. The regions 4, 124 have a density of about 5000 pieces / square centimeter, and follow the regular pattern according to the biochemical analysis units 1, 121.
Formed on the biochemical analysis unit 1, according to a regular pattern.
It is not always necessary to form 121.

Further, in the above embodiment, chemiluminescence is generated by contacting with a substance of biological origin labeled with a radioactive labeling substance, a substance of biological origin labeled with a fluorescent substance such as a fluorescent dye, and a chemiluminescent substrate. A hybridizing solution 9 containing a biologically-derived substance labeled with a labeling substance is prepared and hybridized with the specific binding substance dropped on the adsorptive membrane region 4, but the biologically-derived substance is radioactively labeled. It is not always necessary that the substance, a fluorescent substance such as a fluorescent dye, and a labeling substance that causes chemiluminescence when brought into contact with a chemiluminescent substrate are labeled, and in addition to the radioactive labeling substance or the radioactive labeling substance, the fluorescent substance and At least one of the labeling substances that produces chemiluminescence when contacted with a chemiluminescent substrate Only it needs to be labeled by one of the labeled substance.

Furthermore, in the above-mentioned embodiment, a substance derived from a living body which is labeled with a radioactive labeling substance, a fluorescent substance such as a fluorescent dye, and a labeling substance which produces chemiluminescence by contacting with a chemiluminescent substrate is specifically bound. Although it is hybridized to the substance,
It is not always necessary to hybridize to a specific binding substance, and a substance of biological origin is specifically bound to a specific binding substance by an antigen-antibody reaction, a reaction such as a receptor / ligand, etc., instead of hybridization. You can also let it.

Further, in the above embodiment, the biochemical analysis units 1 and 121 are formed in the same regular pattern as the absorptive membrane regions 4 and 124 formed in the biochemical analysis units 1 and 121. Adsorbent membrane regions 4, 12
4, a substantially circular stimulable phosphor layer region 12, 132 having a size equal to 4 is formed on the support 11, 131 of the stimulable phosphor sheet 10, 130. , 132 are biochemical analysis units 1, 121
It is not always necessary to have the same size as the absorptive membrane regions 4 and 124 formed in 1., and preferably the size of the absorptive membrane regions 4 and 124 formed in the biochemical analysis unit 1 and 121. The above is formed.

In the above embodiment, the absorptive membrane region 4 formed in the biochemical analysis unit 1, 121,
A substantially circular stimulable phosphor layer region 1 having the same regular pattern as 124 and having the same size as the absorptive film regions 4 and 124 formed in the biochemical analysis units 1 and 121.
2, 132 are formed on the supports 11, 131 of the stimulable phosphor sheets 10, 130, but the stimulable phosphor layer regions 12, 132 are formed on the biochemical analysis unit 1, 121. It is sufficient if it is formed in the same pattern as the absorptive film regions 4 and 124, and it is not always necessary to form it regularly.

Further, in the above embodiment, the biochemical analysis units 1 and 121 are formed in the same regular pattern as the absorptive membrane regions 4 and 124 formed in the biochemical analysis units 1 and 121. Adsorbent membrane regions 4, 12
4, a substantially circular stimulable phosphor layer region 12, 132 having a size equal to 4 is formed on the support 11, 131 of the stimulable phosphor sheet 10, 130. , 132 are not necessarily formed in a substantially circular shape, and may be formed in an arbitrary shape, for example, a rectangular shape.

Furthermore, in the above embodiment, the supports 11, 131 of the stimulable phosphor sheets 10, 130 are made of stainless steel, but the supports 11, 131 of the stimulable phosphor sheets 10, 130 are not. It is not always necessary that it is made of stainless steel, and preferably, the support 11 for the stimulable phosphor sheets 10 and 130,
131 is formed of a material having a property of attenuating radiation, but is not particularly limited, and either an inorganic compound material or an organic compound material can be used, but a metal material, a ceramic material, or a plastic material can be used. Materials are preferably used. Examples of the inorganic compound material preferably used to form the supports 11 and 131 of the stimulable phosphor sheets 10 and 130 include, for example,
Gold, silver, copper, zinc, aluminum, titanium, tantalum,
Metals such as chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; aluminum oxide, magnesium oxide , Metal oxides such as zirconium oxide;
Inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, gallium arsenide and the like can be mentioned. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. In addition, the stimulable phosphor sheet 10,
As the organic compound material that is preferably used to form the supports 11 and 131 of 130, a polymer compound is preferably used, and as a preferable polymer compound, for example, polyolefin such as polyethylene or polypropylene; polymethylmethacrylate, Acrylic resin such as butyl acrylate / methyl methacrylate copolymer;
Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyesters such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon 4,10 Nylon such as; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac; Epoxy resin; Polyurethane; Polystyrene; Butadiene-styrene copolymer; Cellulose, Cellulose acetate, Nitrocellulose, Starch, Alginic acid Polysaccharides such as calcium and hydroxypropyl methylcellulose; chitin; chitosan; sumacum; gelatin, collagen, kerula Polyamides such emissions and the like can be mentioned copolymers of these polymeric compounds.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Further, in the above-mentioned embodiment, by using the scanner shown in FIGS. 9 to 16, a large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 are recorded. The radiological data of the radiolabeled substance and the fluorescence data of the fluorescent substance such as the fluorescent dye recorded in the biochemical analysis unit 1 are read to generate the biochemical analysis data. It is not always necessary to read the fluorescence data of the fluorescent substance by one scanner, and the radiation data of the radiolabeled substance and the fluorescence data of the fluorescent substance should be read by separate scanners to generate the data for biochemical analysis. May be.

Further, in the above-mentioned embodiment, the radiation data and biochemical analysis unit 1 of the radiolabeled substance recorded in the large number of dot-shaped stimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10. The scanners shown in FIGS. 9 to 16 are used to read the fluorescence data of fluorescent substances such as fluorescent dyes recorded in the above to generate biochemical analysis data. Alternatively, as a scanner for reading the fluorescence data of the fluorescent substance, a large number of dot-shaped stimulable phosphor layer regions 12 or the surface of the biochemical analysis unit 1 are scanned with the laser beam 24 or the excitation light to stimulate the stimulable substance. Any substance capable of exciting a fluorescent substance or a fluorescent substance may be used, and the emission of the radiolabeled substance may be performed using the scanner shown in FIGS. 9 to 16. It is not necessarily required to read fluorescence data of the data or the fluorescent substance.

Further, the scanner shown in FIGS. 9 to 16 includes a first laser excitation light source 21, a second laser excitation light source 22 and a third laser excitation light source 23, but three laser excitation light sources are used. It is not always necessary to have a light source.

Further, in the above-mentioned embodiment, a large number of absorptive membrane regions 4 formed in the biochemical analysis unit 1 are formed by the data generation system shown in FIGS. The data for biochemical analysis is generated by reading the chemiluminescence data of the labeled substance that causes chemiluminescence by contact with the recorded chemiluminescence substrate, but the data for biochemical analysis is read by reading the chemiluminescence data. It is not always necessary that the data generation system that generates
The data generation system exclusively reads only the chemiluminescence data of the labeling substance that causes chemiluminescence by contacting with the chemiluminescence substrate recorded in the large number of adsorptive membrane regions 4 formed in the biochemical analysis unit 1. LED light source 100, filter 10 when used for
1, the filter 102 and the diffusion plate 103 can be omitted.

In the above embodiment, the optical head 3 is moved by the scanning mechanism in the main scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG.
5 is moved to scan all dot-shaped stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 or the entire surface of the biochemical analysis unit 1 with the laser light 24, thereby stimulating the stimulable phosphor. Alternatively, although a fluorescent substance such as a fluorescent dye is excited, the optical head 35 is kept stationary, and the stage 40 is moved in the main scanning direction indicated by arrow X and the sub scanning direction indicated by arrow Y in FIG. All the dot-shaped stimulable phosphor layer regions 1 of the stimulable phosphor sheet 10 are moved by the laser light 24 by moving.
2 or scan the entire surface of the biochemical analysis unit 1,
A fluorescent substance such as a stimulable fluorescent substance or a fluorescent dye may be excited, and the optical head 35 may be arranged as shown in FIG.
In the main scanning direction indicated by arrow X or arrow Y
It is possible to move the stage 40 in the sub-scanning direction indicated by arrow Y or in the main-scanning direction indicated by arrow X while moving in the sub-scanning direction indicated by.

Further, in the scanner shown in FIGS. 9 to 16, the photomultiplier 50 is used as the photodetector to detect fluorescence or stimulated emission photoelectrically. The photodetector used is not limited to the photomultiplier 50 and may be a line CCD as long as it can photoelectrically detect fluorescence or photostimulated light.
Other photodetectors such as a two-dimensional CCD or the like can also be used.

Further, in the above-mentioned embodiment, the spotting device equipped with the injector 6 and the CCD camera 7 is used, and the tip of the injector 6 and the specific binding substance such as cDNA should be dropped by the CCD camera 7. While observing the membrane region 4, when the tip portion of the injector 6 and the center of the adsorptive membrane region 4 to which a specific binding substance such as cDNA should be dropped match with each other, the injector 6 causes specific binding of cDNA or the like. Although the substance is released and dropped, the relative positional relationship between the tip of the injector 6 and the large number of absorptive film regions 4 formed in the biochemical analysis unit 1 is detected in advance. , The injector 6 and the biochemical analysis unit 1 are relatively two-dimensionally moved at a constant pitch to drop a specific binding substance such as cDNA. It can also be adapted to.

[0337]

INDUSTRIAL APPLICABILITY According to the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. is spotted on a carrier. Even when a high-density spot-like region is formed by dropping, a substance of biological origin labeled with a radiolabeling substance is specifically bound to the spot-like specific binding substance to selectively label it. The biochemical analysis unit obtained as described above is brought into close contact with the stimulable phosphor layer, and the stimulable phosphor layer is exposed to a radioactive labeling substance. When photoelectrically detecting the photostimulable light emitted from the photostimulable phosphor layer, the photostimulable light can be detected with high resolution, and it is possible to generate biochemical analysis data with excellent quantitativeness. To provide a biochemical analysis unit and a method for manufacturing the same It becomes ability.

Furthermore, according to the present invention, it is possible to specifically bind to a substance of biological origin, and the base sequence and the length of the base,
Specific binding substance having a known composition and the like is dropped onto a carrier in a spot shape, and even when a high-density spot-shaped region is formed, a spot-shaped specific binding substance is added to a radiolabeled substance, Alternatively, instead of the radiolabeled substance,
From a biochemical analysis unit obtained by selectively binding a biogenic substance labeled with a labeling substance and / or a fluorescent substance, which causes chemiluminescence by contacting with a chemiluminescent substrate, and selectively labeling When the emitted chemiluminescence and / or fluorescence is detected photoelectrically, the chemiluminescence and / or fluorescence can be detected with high resolution, and biochemical analysis data with excellent quantitativeness can be generated. It is possible to provide a biochemical analysis unit and a manufacturing method thereof.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a partial cross-sectional view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 3 is a process drawing showing an example of a method for producing an adsorptive film.

FIG. 4 is a schematic cross-sectional view of a calendering apparatus for manufacturing a biochemical analysis unit.

FIG. 5 is a schematic front view of a spotting device.

FIG. 6 is a schematic cross-sectional view of a hybridization container.

FIG. 7 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 8 shows a large number of dot-shaped stimulable phosphor layers formed on a stimulable phosphor sheet by a radioactive labeling substance contained in a large number of porous regions formed in a biochemical analysis unit. It is a schematic sectional drawing which shows the method of exposing an area | region.

FIG. 9 is a schematic perspective view showing an example of a scanner.

FIG. 10 is a schematic perspective view showing details of a scanner near the photomultiplier.

11 is a schematic cross-sectional view taken along the line AA of FIG.

12 is a schematic cross-sectional view taken along the line BB of FIG.

FIG. 13 is a schematic cross-sectional view taken along the line CC of FIG.

FIG. 14 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 15 is a schematic plan view of a scanning mechanism of an optical head.

16 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner shown in FIG.

FIG. 17 is a diagram showing the results obtained by reading chemiluminescence data of a labeling substance that causes chemiluminescence by contacting with a chemiluminescence substrate recorded in a large number of porous regions formed in a biochemical analysis unit, 1 is a schematic front view of a data generation system that generates chemical analysis data.

FIG. 18 is a cooling CCD of the data generation system.
It is a schematic longitudinal cross-sectional view of a camera.

FIG. 19 is a schematic vertical sectional view of a dark box of the data generation system.

FIG. 20 is a block diagram around a personal computer of the data generation system.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 22 is a schematic partial cross-sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 23 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 24 is a diagram showing a stimulable phosphor sheet formed by a radioactive labeling substance contained in a large number of porous regions formed in a biochemical analysis unit according to another preferred embodiment of the present invention. It is a schematic sectional drawing which shows the method of exposing many dot-shaped photostimulable phosphor layer areas.

FIG. 25 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 26 is a process drawing showing an example of a method for producing an absorptive substrate.

[Explanation of symbols]

1 Biochemical analysis unit 2 Adsorbent substrate 2a nylon 6 fiber 2b Hot melt adhesive 2c compressed body 3 adhesive 4 Adsorbent membrane area 5 Aluminum substrate 6 injectors 7 CCD camera 8 Hybridization container 9 Hybridization solution 10 Storage phosphor sheet 11 Support 12 Dot-shaped stimulable phosphor layer region 13 recess 15 calendar rolls 21 First laser excitation light source 22 Second laser excitation light source 23 Third Laser Excitation Light Source 24 laser light 25 Collimator lens 26 mirror 27 First Dichroic Mirror 28 Second dichroic mirror 29 mirror 30 collimator lens 31 Collimator lens 32 mirror 33 holes for the perforated mirror 34 perforated mirror 35 Optical head 36 mirror 37 Aspherical lens 38 concave mirror 40 stages 41 glass plate 45 Fluorescence or stimulated emission 48 filter units 50 Photomultiplier 51a, 51b, 51c, 51d filter member 52a, 52b, 52c, 52d filters 53 A / D converter 54 Data processing device 60 substrates 61 Sub-scanning pulse motor 62 a pair of rails 63 Movable substrate 64 rod 65 Main scanning stepping motor 66 endless belt 67 Linear encoder 68 Linear encoder slit 70 Control unit 71 keyboard 72 Filter unit motor 81 Cooled CCD camera 82 Dark Box 83 personal computer 84 CRT 85 keyboard 86 CCD 87 heat transfer plate 88 Peltier element 89 shutter 90 A / D converter 91 Image data buffer 92 Camera control circuit 95 glass plate 96 radiation fin 97 camera lens 100 LED light source 101 Filter 102 filters 103 Diffuser 110 CPU 111 data transfer means 112 data storage means 113 Data processing device 114 data display means 115 Light source control means 121 Biochemical analysis unit 122 Adsorbent substrate 123 adhesive 124 Adsorbent membrane area 125 plastic substrate 130 Storage phosphor sheet 131 support 132 Dot-shaped stimulable phosphor layer region 141 Biochemical analysis unit 142 Adsorbent substrate 142a nylon 6 fiber 142b Hot melt adhesive 142c Compressed body

Claims (44)

[Claims] 1. An absorptive film formed of an absorptive material is provided, and the absorptive film is compared with a direction parallel to the film surface.
A biochemical analysis unit having a higher liquid permeability in a direction perpendicular to the membrane surface. 2. The absorptive film is composed of a fiber bundle composed of a plurality of fibers, and most of the plurality of fibers forming the fiber bundle are oriented in a direction perpendicular to the film surface. The biochemical analysis unit according to claim 1. 3. The biochemical analysis unit according to claim 2, wherein the fiber bundle is formed by fusing at least a part of the plurality of fibers. 4. The biochemical analysis unit according to claim 2, wherein at least a part of the fiber bundle is impregnated with an adhesive. 5. The biochemical analysis unit according to claim 2, wherein at least a part of the plurality of fibers has a hollow structure along the axis. . 6. A plate-shaped member formed of a material that attenuates radiation and / or light and having a plurality of through holes is embedded in the absorptive film, and the absorptive film is formed by the plate-shaped member. Is divided into a plurality of absorptive film regions separated from each other, and the plurality of absorptive film regions are formed at positions corresponding to the plurality of through holes of the plate-shaped member. The biochemical analysis unit according to any one of 1 to 5. 7. The biochemical analysis unit according to claim 6, wherein the plurality of absorptive film regions are formed by press-fitting the plate-shaped member into the absorptive film. 8. The biochemical analysis according to claim 7, wherein the plurality of absorptive membrane regions are formed by press-fitting the plate-shaped member into the absorptive membrane by calendering. Unit. 9. The adsorbent film region according to claim 7, wherein the plurality of adsorbent film regions are formed by press-fitting the plate-shaped member into the adsorbent film via an adhesive. Biochemical analysis unit. 10. The substrate further comprises a substrate formed of a material that attenuates radiation and / or light and having a plurality of holes formed therein, wherein the adsorptive film is provided with an adsorptive material in the plurality of holes of the substrate. 2. A plurality of adsorptive film regions formed by being embedded are formed.
6. The biochemical analysis unit according to any one of items 1 to 5. 11. A plurality of through holes are formed in the substrate, and the plurality of absorptive film regions are formed by embedding an absorptive material in the plurality of through holes formed in the substrate. The biochemical analysis unit according to claim 10, wherein. 12. The substrate has a plurality of recesses formed therein,
The biochemical analysis unit according to claim 10, wherein the plurality of absorptive film regions are formed by absorptive material being embedded in the plurality of recesses formed in the substrate. 13. The method according to claim 6, wherein 10 or more of the absorptive film regions are formed.
The unit for biochemical analysis according to the item. 14. The biochemical analysis unit according to claim 13, wherein 1000 or more of the absorptive membrane regions are formed. 15. The biochemical analysis unit according to claim 14, wherein 10,000 or more of the absorptive membrane regions are formed. 16. The biochemical analysis unit according to claim 6, wherein each of the plurality of absorptive membrane regions is formed to have a size of less than 5 mm 2. 17. The biochemical analysis unit according to claim 16, wherein each of the plurality of absorptive membrane regions is formed to have a size of less than 1 mm 2. 18. The biochemical analysis unit according to claim 17, wherein each of the plurality of adsorptive membrane regions is formed to have a size of less than 0.1 mm 2. 19. The plurality of adsorptive membrane regions are 10 /
The biochemical analysis unit according to any one of claims 6 to 18, wherein the biochemical analysis unit is formed with a density of not less than square centimeters. 20. The plurality of adsorptive membrane regions are 50 /
The biochemical analysis unit according to claim 19, wherein the biochemical analysis unit is formed with a density of not less than square centimeters. 21. The plurality of adsorptive membrane regions comprises 1000
The biochemical analysis unit according to claim 20, wherein the biochemical analysis unit is formed with a density of not less than one piece / square centimeter. 22. The plurality of adsorptive membrane regions comprises 1000
The biochemical analysis unit according to claim 21, wherein the biochemical analysis unit is formed with a density of 0 pieces / square centimeter or more. 23. The radiation and / or light attenuating material is such that when the radiation and / or light penetrates through the material a distance equal to the distance between adjacent adsorbent membrane regions. 23. The biochemical analysis unit according to any one of claims 6 to 22, which has a property of attenuating light energy to 1/5 or less. 24. When the radiation and / or light attenuating material transmits radiation and / or light through the material a distance equal to the distance between adjacent adsorbent membrane regions, 24. The biochemical analysis unit according to claim 23, which has a property of attenuating light energy to 1/10 or less. 25. The radiation and / or light attenuating material is such that when the radiation and / or light penetrates through the material a distance equal to the distance between adjacent adsorbent membrane regions. 25. The biochemical analysis unit according to claim 24, which has a property of attenuating light energy to 1/100 or less. 26. The method according to claim 23, wherein the radiation and / or light attenuating material is a material selected from the group consisting of a metal material, a ceramic material and a plastic material. Biochemical analysis unit described. 27. The raw material according to any one of claims 1 and 6 to 26, wherein the adsorbent material includes a porous carbon material or a porous material capable of forming a membrane filter. Chemical analysis unit. 28. A method for manufacturing a biochemical analysis unit having an adsorptive membrane, comprising a fiber bundle comprising a plurality of fibers,
A method for manufacturing a biochemical analysis unit, characterized in that most of the plurality of fibers are arranged so as to face a direction perpendicular to the film surface of the adsorptive film to form the adsorptive film. 29. The method for manufacturing a biochemical analysis unit according to claim 28, wherein at least a part of the plurality of fibers is fused to form the fiber bundle. 30. At least a portion of the plurality of fibers,
29. The method for manufacturing a biochemical analysis unit according to claim 28, wherein the fiber bundle is formed by impregnating an adhesive. 31. At least a portion of the plurality of fibers is
The biochemical analysis unit manufacturing method according to any one of claims 28 to 30, wherein the biochemical analysis unit has a hollow structure along the axis. 32. A plate-shaped member, which is formed of a material that attenuates radiation and / or light and has a plurality of through holes, is embedded in the adsorptive film, and is separated from each other in the adsorptive film region, 32. The biochemical analysis unit according to claim 28, wherein a plurality of absorptive film regions located at positions corresponding to the plurality of through holes of the plate-like member are formed. Production method. 33. The method for manufacturing a biochemical analysis unit according to claim 32, wherein the plate-shaped member is press-fitted into the absorptive film to form the plurality of absorptive film regions. 34. The biochemical analysis unit according to claim 33, wherein the plate-shaped member is press-fitted into the adsorptive film by calendering to form the plurality of adsorptive film regions. Manufacturing method. 35. The raw material according to claim 33, wherein the plate-shaped member is press-fitted into the adsorptive film via an adhesive to form the plurality of adsorptive film regions. Manufacturing method of chemical analysis unit. 36. Further, a substrate formed of a material that attenuates radiation and / or light and provided with a plurality of holes is prepared, and a fiber bundle composed of a plurality of fibers is provided in the plurality of holes formed in the substrate. Is embedded so that many of the plurality of fibers are oriented in a direction perpendicular to the film surface of the adsorptive film to form a plurality of adsorptive film regions to form the adsorptive film. The method for manufacturing a biochemical analysis unit according to any one of claims 28 to 31. 37. A plurality of through holes are formed in the substrate,
37. The method for manufacturing a biochemical analysis unit according to claim 36, wherein an adsorbent material is embedded in the through holes formed in the substrate to form the adsorbent film regions. 38. A plurality of recesses are formed in the substrate, and an adsorptive material is embedded in the plurality of recesses formed in the substrate to form the plurality of adsorptive film regions. Item 36. A method for manufacturing a biochemical analysis unit according to Item 36. 39. The method according to claim 32, wherein 10 or more of the adsorptive film regions are formed.
A method for manufacturing the biochemical analysis unit according to the item. 40. The biochemical analysis unit according to claim 32, wherein each of the plurality of absorptive membrane regions has a size of less than 5 mm 2. Method. 41. The number of the plurality of adsorptive membrane regions is 10 /
The method for manufacturing a biochemical analysis unit according to any one of claims 32 to 40, wherein the biochemical analysis unit is formed with a density of not less than square centimeters. 42. The radiation and / or light attenuating material is such that when the radiation and / or light penetrates through the material a distance equal to the distance between adjacent adsorbent membrane regions. 42. The method for manufacturing a biochemical analysis unit according to any one of claims 32 to 41, which has a property of attenuating light energy to 1/5 or less. 43. The biochemical analysis unit according to claim 42, wherein the material that attenuates the radiation and / or the light is a material selected from the group consisting of a metal material, a ceramic material and a plastic material. Manufacturing method. 44. The biochemical analysis according to claim 28, wherein the adsorptive material contains a porous carbon material or a porous material capable of forming a membrane filter. Method for manufacturing units.