JP4153229B2 - Stimulable phosphor sheet and method for reading data for biochemical analysis recorded on the stimulable phosphor sheet - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a stimulable phosphor sheet and a method for reading data for biochemical analysis recorded on the stimulable phosphor sheet, and more specifically, capable of specifically binding to a substance derived from a living body, In addition, a plurality of spot-like regions containing specific binding substances with known base sequences, base lengths, compositions, etc., are formed on the carrier surface at high density, and the specific binding contained in the plurality of spot-like regions. For biochemical analysis with high resolution and excellent quantification even when a biologically-derived substance labeled with a radioactive labeling substance is specifically bound to the substance and multiple spot-like regions are selectively labeled. The present invention relates to a stimulable phosphor sheet capable of generating the above data and a method for reading data for biochemical analysis recorded on the stimulable phosphor sheet.
[0002]
[Prior art]
When irradiated with radiation, the energy of the radiation is absorbed, stored, recorded, and then excited using electromagnetic waves in a specific wavelength range. After using a stimulable phosphor having the property of emitting light as a radiation detection material, a radioactively labeled substance is administered to the organism, and then the organism or a part of the tissue of the organism is used as a sample. The sample is superposed on a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time, whereby radiation energy is accumulated and recorded in the stimulable phosphor, and then the sample is illuminated by electromagnetic waves. The stimulable phosphor layer is scanned to excite the stimulable phosphor, and the photostimulated light emitted from the stimulable phosphor is photoelectrically detected to generate a digital image signal and perform image processing. On the display means such as CRT Or, an autoradiography analysis system configured to reproduce an image on a recording material such as a photographic film is known (for example, Japanese Patent Publication No. 1-70884, Japanese Patent Publication No. 1-70882, No. 4-3962).
[0003]
Unlike radiographic systems, autoradiographic analysis systems that use stimulable phosphor sheets as radiation detection materials do not require chemical processing, such as development processing, but can also be applied to the resulting digital data. By performing the data processing, there is an advantage that the data for analysis can be reproduced as desired or quantitative analysis by a computer can be performed.
[0004]
On the other hand, a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system is known. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, gene sequence, gene expression level, metabolism, absorption, excretion route, state of the administered substance in the experimental mouse, separation of proteins, Identification, molecular weight, property evaluation, etc. can be performed. For example, after a solution containing multiple types of protein molecules to be electrophoresed is electrophoresed on a gel support, the gel support is fluorescent. Stain the electrophoretic protein by soaking in a solution containing the dye, etc., excite the fluorescent dye with excitation light, and detect the resulting fluorescence to generate an image on the gel support The position and quantitative distribution of protein molecules can be detected. Alternatively, at least a portion of the electrophoresed protein molecule is transferred onto a transfer support such as nitrocellulose by Western blotting, and an antibody that specifically reacts with the target protein is labeled with a fluorescent dye. By associating the prepared probe with the protein molecule, selectively labeling the protein molecule that binds only to the antibody that reacts specifically, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, An image can be generated to detect the location and quantitative distribution of protein molecules on the transfer support. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. Electrophoresis of a plurality of DNA fragments or electrophoresis of a plurality of DNA fragments on a gel support followed by immersing the gel support in a solution containing a fluorescent dye. By labeling the DNA fragments, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, an image is generated and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected. After the fragments are electrophoresed on a gel support, the DNA is denaturated, and then at least the denatured DNA fragments are transferred onto a transfer support such as nitrocellulose by Southern blotting. In addition, a probe prepared by transferring a part of the DNA and labeling the DNA or RNA complementary to the target DNA with a fluorescent dye is hybridized with the denatured DNA fragment, and only the DNA fragment complementary to the probe DNA or probe RNA. Can be selectively labeled, the fluorescent dye can be excited with excitation light, and the resulting fluorescence can be detected to generate an image and detect the distribution of the target DNA on the transfer support. it can. Further, a DNA probe complementary to the DNA containing the target gene labeled with the labeling substance is prepared, hybridized with the DNA on the transcription support, and the enzyme is combined with the complementary DNA labeled with the labeling substance. After binding, contact the fluorescent substrate, change the fluorescent substrate into a fluorescent substance that emits fluorescence, excite the generated fluorescent substance with excitation light, and generate the image by detecting the generated fluorescence It is also possible to detect the distribution of the target DNA on the transfer support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using a radioactive substance.
[0005]
Similarly, biologically-derived substances such as proteins and nucleic acids are immobilized on a support, selectively labeled with a labeling substance that generates chemiluminescence by contacting with a chemiluminescent substrate, and selectively selected with a labeling substance. A chemiluminescent substrate in contact with a labeled biological substance and a chemiluminescent substrate is contacted, and the chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeling substance is photoelectrically detected to generate a digital image signal. There is also a chemiluminescence analysis system that performs image processing and reproduces a chemiluminescence image on a display means such as a CRT or a recording material such as a photographic film to obtain information on a substance derived from a living body such as genetic information. Are known.
[0006]
Furthermore, in recent years, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA, RNA, etc. can be placed at different positions on the surface of a carrier such as a glass slide or membrane filter. A specific binding substance that can specifically bind to a substance derived from a living body and has a known base sequence, base length, composition, etc., is dropped using a spotter device, and a large number of independent spots. And then collected from a living body by extraction, isolation, etc., such as cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA, mRNA, etc. Furthermore, it is a biological material that has been subjected to chemical treatment, chemical modification, etc. A microarray in which the labeled substance is specifically bound to a specific binding substance by hybridization or the like is irradiated with excitation light, and fluorescence emitted from the labeling substance such as a fluorescent substance or a dye A microarray analysis system has been developed which detects light photoelectrically and analyzes a substance derived from a living body. According to this microarray analysis system, a large number of spots of specific binding substances are formed in high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a biological substance labeled with a labeling substance. By hybridizing, there is an advantage that it becomes possible to analyze a substance derived from a living body in a short time.
[0007]
In addition, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA, RNA, A specific binding substance that can be specifically bound and has a known base sequence, base length, composition, and the like is dropped by using a spotter device to form a large number of independent spots, Cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA, mRNA, etc., collected from living organisms by extraction, isolation, etc., or further chemically treated , A biologically-derived substance that has been subjected to treatment such as chemical modification and that has been labeled with a radioactive labeling substance. The macroarray that is specifically bound to the specific binding substance by means of, for example, a close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed is The photosensitive phosphor layer is exposed, and after that, the stimulable phosphor layer is irradiated with excitation light, and the stimulating light emitted from the stimulable phosphor layer is detected photoelectrically, and data for biochemical analysis is obtained. A macroarray analysis system using a radiolabeled substance that generates a lysine and analyzes a substance derived from a living body has also been developed.
[0008]
[Problems to be solved by the invention]
However, in a macroarray analysis system using a radiolabeled substance as a labeling substance, when the photostimulable phosphor layer is exposed, the radioactivity contained in the spot-like region formed on the carrier surface such as a membrane filter is exposed. Since the radiation energy of the labeling substance is very large, the photostimulable phosphor to be exposed by the radioactive labeling substance contained in the adjacent spot-like region is scattered by the electron beam (β-ray) emitted from the radiolabeling substance Electron beam (β-ray) emitted from the radioactive labeling substance incident on the layer area or adhering on the surface of the carrier such as a membrane filter between adjacent spot-shaped areas is incident on the stimulable phosphor layer As a result, noise is generated in the biochemical analysis data generated by photoelectrically detecting the stimulated light, and it is difficult to separate the data between adjacent spot regions. It becomes difficult, the resolution is lowered, and there is a problem that the quantitative property deteriorates when analyzing the substance derived from the living body by quantifying the radiation dose of each spot-like region, and the spot-like regions are formed close to each other When the density is to be increased, particularly, the resolution is remarkably lowered and the quantitative property is markedly deteriorated.
[0009]
Therefore, in the present invention, a plurality of spot-like regions containing a specific binding substance that can specifically bind to a substance derived from a living body and has a known base sequence, base length, composition, etc. are provided on the surface of the carrier. A specific binding substance that is formed in high density and contained in multiple spot-like areas is specifically bound to a biologically-derived substance labeled with a radiolabeled substance to selectively label multiple spot-like areas. In this case, it is possible to generate biochemical analysis data with excellent resolution and high resolution, and a method for reading the data for biochemical analysis recorded on the storage phosphor sheet. Is intended to provide.
[0010]
[Means for Solving the Problems]
An object of the present invention is to provide a support, and a plurality of photostimulable phosphor layer regions are formed on the support so as to be spaced apart from each other, and are further spaced from the plurality of photostimulable phosphor layer regions. The storage phosphor sheet is characterized in that at least one additional photostimulable phosphor layer region is formed.
[0011]
According to the present invention, a plurality of spot-like regions containing a specific binding substance that can specifically bind to a substance derived from a living body and has a known base sequence, base length, composition, etc., can be used as a membrane filter or the like. A plurality of spot-like regions are formed by specifically binding a biologically-derived substance labeled with a radioactive labeling substance to a specific binding substance formed on the carrier surface at a high density and contained in a plurality of spot-like areas. Even in the case of selective labeling, by forming a plurality of photostimulable phosphor layer regions on the support in the same pattern as a plurality of spot-like regions formed on the carrier, the carrier and the stimulable phosphor sheet When a plurality of photostimulable phosphor layers are exposed by a radiolabeling substance selectively contained in a plurality of spot-like areas, the radioactive labeling substances contained in each spot-like area are exposed. The photostimulable fluorescence other than the photostimulable phosphor layer region where the emitted electron beam (β-ray) is to be exposed by the electron beam (β-ray) emitted from the radioactive label contained in the spot-like region. It is possible to effectively prevent the light from entering the body layer region. Therefore, the plurality of photostimulable phosphor layer regions exposed are scanned with the excitation light and emitted from the plurality of photostimulable phosphor layer regions. By detecting the photostimulated light photoelectrically, it is possible to generate data for biochemical analysis with high resolution and excellent quantification.
[0012]
Furthermore, a carrier such as a membrane filter in which a plurality of spot-like regions selectively containing a radioactive labeling substance is formed and a storage phosphor sheet in which a plurality of photostimulable phosphor layer regions are formed are superimposed, A plurality of photostimulable phosphors formed on a support of a stimulable phosphor sheet when a plurality of photostimulable phosphor layer regions are exposed by a radiolabeling substance selectively contained in a plurality of spot-like regions In the layer area, not only the electron beam (β-ray) emitted from the radiolabeled substance selectively contained in the spot-like area formed on the carrier, but also attached to the surface of the carrier by hybridization etc. and washed. Electron beams (β rays) emitted from the remaining radioactive labeling substance and environmental radiation are also incident, so that the stimulable phosphor layers are exposed to multiple stimulable phosphor layers in the exposed stimulable phosphor sheet. Run by The data for biochemical analysis generated by photoelectrically detecting the photostimulated light emitted from multiple photostimulable phosphor layers is attached to the surface of the carrier by hybridization, etc. In addition, electron beams (β rays) emitted from the remaining radiolabeled substance, environmental radiation, etc. are incident on a plurality of photostimulable phosphor layer regions formed on the support of the stimulable phosphor sheet. Background noise resulting from the above will inevitably be included, but according to the present invention, the support of the stimulable phosphor sheet is further separated from the plurality of stimulable phosphor layer regions, At least one additional photostimulable phosphor layer region is formed, and at least one additional photostimulable phosphor layer region includes a radioactive material selectively contained in a spot-like region formed on the carrier. Released from the labeling substance The electron beam (β-ray), environmental radiation, etc. emitted from the radiolabeled substance that remains on the surface of the carrier due to hybridization etc. and remains after washing is not incident on the child beam (β-ray). Since it is incident and exposed, the at least one additional photostimulable phosphor layer region is scanned with excitation light, and the photostimulated light emitted from the at least one additional photostimulable phosphor layer region is photoelectrically detected. The biochemical analysis data generated by the detection in response to the background noise, therefore, the plurality of photostimulable phosphor layer regions of the exposed stimulable phosphor sheet are scanned with excitation light, At least one additional stimulable phosphor layer region is scanned with excitation light from biochemical analysis data generated by photoelectrically detecting the stimulated light emitted from the photostimulable phosphor layer region. Generating biochemical analysis data free of background noise by subtracting data generated by photoelectrically detecting photostimulated light emitted from at least one additional photostimulable phosphor layer region Is possible.
[0013]
The object of the present invention is also provided with a support, and a plurality of photostimulable phosphor layer regions are formed on the support so as to be spaced apart from each other, and are further spaced from the plurality of photostimulable phosphor layer regions. Then, a stimulable phosphor sheet in which at least one additional stimulable phosphor layer region is formed, and a specific binding substance having a known structure or characteristic are dropped, and a radiolabeling substance is added to the specific binding substance. A plurality of spot-like substances are superimposed on a biochemical analysis unit having a plurality of spot-like regions formed by selectively binding a substance derived from a living body selectively labeled and selectively labeled. The plurality of photostimulable phosphor layers of the stimulable phosphor sheet are exposed to the plurality of photostimulable phosphor sheets after being exposed to the radiolabeling substance selectively contained in the region. Phosphor layer region and front The at least one additional photostimulable phosphor layer region is irradiated with excitation light, and the phosphors contained in the plurality of photostimulable phosphor layer regions and the at least one additional photostimulable phosphor layer region are illuminated. Exciting the stimulable phosphor, photoelectrically detecting the photostimulated light emitted from the photostimulable phosphor, digitizing the obtained analog data, generating digital data, the plurality of photostimulable Excitation light is irradiated to the phosphor layer region, and the photostimulated light emitted from the photostimulable phosphor is detected photoelectrically, and the at least one additional photostimulable phosphor is obtained from the obtained digital data. Irradiate the layer area with excitation light, photoelectrically detect the photostimulated light emitted from the photostimulable phosphor, and subtract the obtained digital data to generate biochemical analysis data. For biochemical analysis recorded on characteristic storage phosphor sheet It is achieved by method of reading data.
[0014]
A specific binding substance having a known structure or characteristic is dropped, and a biologically-derived substance labeled with a radioactive labeling substance is selectively and specifically bound to the specific binding substance by hybridization or the like. A storage phosphor sheet comprising a biochemical analysis unit having a plurality of spot-like regions formed by labeling and a support, and a plurality of photostimulable phosphor layer regions formed on the support And a sub-stimulable phosphor layer region formed on the stimulable phosphor sheet by the radiolabeled substance selectively contained in the plurality of spot-like regions formed in the biochemical analysis unit. In the plurality of photostimulable phosphor layer regions formed on the support of the stimulable phosphor sheet, the radioactive label contained in the plurality of spots formed in the biochemical analysis unit is exposed. Not only the electron beam emitted from the quality, but also from the radiolabeled substance that remains on the surface of the unit for biochemical analysis due to hybridization, etc. A plurality of photostimulable phosphors are formed by scanning the plurality of photostimulable phosphor layer regions that are formed on the support of the stimulable phosphor sheet and exposed to the excitation light because electron beams and environmental radiation are also incident. The data for biochemical analysis generated by photoelectrically detecting the photostimulated light emitted from the layer region is applied to regions other than the multiple spot-like regions on the surface of the biochemical analysis unit by hybridization or the like. A plurality of photostimulable phosphors that are formed on the support of the stimulable phosphor sheet, such as electron beams emitted from radioactively labeled substances that remain attached and washed, and environmental radiation According to the present invention, the support of the stimulable phosphor sheet further includes a plurality of stimulable phosphor layer regions. At least one additional photostimulable phosphor layer region is formed apart from the plurality of spots formed in the biochemical analysis unit in at least one additional photostimulable phosphor layer region. The electron beam emitted from the radiolabeled material contained in is not incident on the surface of the biochemical analysis unit, but adheres to areas other than multiple spots and remains after cleaning. Since the emitted electron beam or ambient radiation is incident and exposed, at least one additional photostimulable phosphor layer is scanned with excitation light, and at least one additional photostimulable phosphor layer is scanned. Released from The data for biochemical analysis generated by photoelectrically detecting the emitted photostimulated light corresponds to background noise, and thus includes a support, and the support includes a plurality of photostimulable phosphor layers. A stimulable phosphor sheet formed with regions spaced apart from each other and further formed with at least one additional stimulable phosphor layer region spaced from a plurality of stimulable phosphor layer regions; Alternatively, a specific binding substance having a known property is dropped, and a biologically-derived substance labeled with a radioactive labeling substance is selectively and specifically bound to the specific binding substance by hybridization or the like. By labeling, the biochemical analysis unit having a plurality of formed spot-like regions is overlapped and selectively included in the plurality of spot-like regions formed in the biochemical analysis unit. After exposing a plurality of photostimulable phosphor layer regions formed on the stimulable phosphor sheet with a radioactive labeling substance, a plurality of photostimulable phosphor layer regions and at least one additional photostimulable phosphor layer The region is irradiated with excitation light to excite the photostimulable phosphor contained in the plurality of photostimulable phosphor layer regions and at least one additional photostimulable phosphor layer region. The photostimulated light emitted from the photocathode is detected photoelectrically, the obtained analog data is digitized, digital data is generated, and the stimulable light is irradiated to multiple photostimulable phosphor layer regions. The photostimulated light emitted from the phosphor is photoelectrically detected, and the obtained digital data is used to irradiate at least one additional photostimulable phosphor layer region with excitation light, and from the photostimulable phosphor. The resulting digital light is detected photoelectrically. By subtracting the data, it is possible to produce biochemical analysis data having no background noise.
[0015]
In a preferred embodiment of the present invention, a plurality of holes are formed in the support of the stimulable phosphor sheet so as to be separated from each other, and the photostimulable phosphor layer region is formed in the plurality of holes. It is formed by filling a photostimulable phosphor.
[0016]
In a further preferred embodiment of the present invention, a plurality of through holes are formed in the support of the stimulable phosphor sheet so as to be spaced apart from each other, and the stimulable phosphor layer region is formed of the plurality of through holes. It is formed by embedding photostimulable phosphors in the holes.
[0017]
In another preferred embodiment of the present invention, a plurality of through holes are formed in the support of the stimulable phosphor sheet so as to be spaced apart from each other, and the plurality of stimuli of the stimulable phosphor sheet are formed. The phosphor layer region is formed by press-fitting a stimulable phosphor film containing a stimulable phosphor into the plurality of through holes.
[0018]
In another preferred embodiment of the present invention, a plurality of recesses are formed on the support of the stimulable phosphor sheet so as to be separated from each other, and the stimulable phosphor layer region is formed of the plurality of recesses. It is formed by embedding a photostimulable phosphor inside.
[0019]
In another preferred embodiment of the present invention, the plurality of photostimulable phosphor layer regions are formed on the surface of the support of the stimulable phosphor sheet.
[0020]
In a preferred embodiment of the present invention, the plurality of photostimulable phosphor layer regions of the stimulable phosphor sheet are formed in dots on the support.
[0021]
In a preferred embodiment of the present invention, each of the plurality of photostimulable phosphor layer regions of the stimulable phosphor sheet is formed in a substantially circular shape.
[0022]
In a preferred embodiment of the present invention, a plurality of additional photostimulable phosphor layer regions are formed in dots on the support of the stimulable phosphor sheet.
[0023]
In a further preferred embodiment of the present invention, a plurality of additional photostimulable phosphor layer regions are provided on the support of the stimulable phosphor sheet between at least a part of the plurality of photostimulable phosphor regions. It is formed in a dot shape.
[0024]
The background noise varies depending on the position on the surface of the stimulable phosphor sheet, that is, each of the plurality of photostimulable phosphor regions, but according to a preferred embodiment of the present invention, the plurality of photostimulable phosphor regions Since a plurality of additional photostimulable phosphor layers are formed in dots on the support between at least a part of the substrate, the background noise varies depending on the position on the surface of the stimulable phosphor sheet. Even so, it is possible to accurately generate biochemical analysis data from which background noise has been removed.
[0025]
In another preferred embodiment of the present invention, the at least one additional stimulable phosphor layer region of the stimulable phosphor sheet is formed in stripes on the support.
[0026]
In a further preferred embodiment of the present invention, a plurality of additional photostimulable phosphor layer regions are provided on the support between at least a part of the plurality of photostimulable phosphor regions of the stimulable phosphor sheet. , Are formed in stripes.
[0027]
The background noise varies depending on the position on the surface of the stimulable phosphor sheet, that is, each of the plurality of photostimulable phosphor regions, but according to a further preferred embodiment of the present invention, the plurality of photostimulable phosphors Since a plurality of additional photostimulable phosphor layer regions are formed in stripes on the support between at least a part of the regions, background noise may be generated depending on the position on the surface of the stimulable phosphor sheet. Even if it fluctuates, it is possible to accurately generate digital data for biochemical analysis from which background noise has been removed.
[0028]
In a preferred embodiment of the present invention, the additional stimulable phosphor layer region of the stimulable phosphor sheet is formed in a substantially circular shape on the support.
[0029]
In a preferred embodiment of the present invention, the area of the additional stimulable phosphor layer region of the stimulable phosphor sheet is smaller than the area of each of the plurality of stimulable phosphor layer regions. Further, it is formed on the support.
[0030]
In a preferred embodiment of the present invention, the support of the stimulable phosphor sheet is made of a material that attenuates radiation.
[0031]
According to a preferred embodiment of the present invention, a plurality of spot-like regions containing a specific binding substance that can specifically bind to a substance derived from a living body and has a known base sequence, base length, composition, etc. A biologically-derived substance labeled with a radioactive labeling substance is specifically bound to a specific binding substance formed on a carrier surface such as a membrane filter at a high density and contained in a plurality of spot-like regions. Even when the spot-like regions are selectively labeled, the plurality of photostimulable phosphor layer regions are formed on the support in the same pattern as the plurality of spot-like regions formed on the carrier, thereby accumulating with the carrier. When a plurality of photostimulable phosphor layer regions are exposed by a radiolabeling substance selectively contained in a plurality of spot-like regions by overlapping the fluorescent phosphor sheets, they are contained in each spot-like region. The electron beam (β-ray) emitted from the radiolabeled substance is exposed to an electron beam (β-ray) emitted from the radiolabeled substance contained in the spot-like region, except for the photostimulable phosphor layer region to be exposed. It is possible to effectively prevent the light from entering the photostimulable phosphor layer region. Therefore, the plurality of photostimulable phosphor layer regions that are exposed are scanned with the excitation light, and the plurality of photostimulable phosphor layers are scanned. By detecting the photostimulated light emitted from the region photoelectrically, it is possible to generate data for biochemical analysis with high resolution and excellent quantitativeness.
[0032]
In a preferred embodiment of the present invention, when the support of the stimulable phosphor sheet transmits radiation through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. The material is formed of a material having a property of attenuating radiation energy to 1/5 or less.
[0033]
In a further preferred embodiment of the present invention, when the support of the stimulable phosphor sheet has radiation transmitted through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. Furthermore, it is made of a material having a property of attenuating radiation energy to 1/10 or less.
[0034]
In a further preferred embodiment of the present invention, when the support of the stimulable phosphor sheet has radiation transmitted through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. In addition, it is made of a material having a property of attenuating radiation energy to 1/50 or less.
[0035]
In a further preferred embodiment of the present invention, when the support of the stimulable phosphor sheet has radiation transmitted through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. In addition, it is made of a material having a property of attenuating radiation energy to 1/100 or less.
[0036]
In a further preferred embodiment of the present invention, when the support of the stimulable phosphor sheet has radiation transmitted through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. In addition, it is made of a material having a property of attenuating radiation energy to 1/500 or less.
[0037]
In a further preferred embodiment of the present invention, when the support of the stimulable phosphor sheet has radiation transmitted through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. In addition, it is made of a material having a property of attenuating radiation energy to 1/1000 or less.
[0038]
In the present invention, the material for forming the support of the stimulable phosphor sheet is preferably a material having a property of attenuating radiation energy, but is not particularly limited, and is an inorganic compound material or an organic compound material. Either can be used, and metallic materials, ceramic materials or plastic materials are particularly preferably used.
[0039]
In the present invention, as an inorganic compound material that can be preferably used to form a support for a stimulable phosphor sheet and can attenuate radiation energy, for example, gold, silver, copper, zinc, aluminum, titanium, Metals such as tantalum, chromium, iron, nickel, cobalt, lead, tin, and selenium; alloys such as brass, stainless steel, and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide, and silicon nitride; aluminum oxide, Examples thereof include metal oxides such as magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.
[0040]
In the present invention, as an organic compound material that can be used to form a support for a stimulable phosphor sheet and can attenuate radiation energy, a polymer compound is preferably used, such as polyethylene and polypropylene. Polyolefins; Acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyethylene naphthalate Polyester such as polyethylene terephthalate; nylon such as nylon 6, nylon 6,6, nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; Silicon resin such as diphenylsiloxane; phenolic resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer; polysaccharide such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropyl methylcellulose; Chitosan; Urushi; Polyamides such as gelatin, collagen, keratin, and copolymers of these polymer compounds. These may be composite materials, and if necessary, may be filled with metal oxide particles or glass fibers, or may be used by blending organic compound materials.
[0041]
In general, the greater the specific gravity, the higher the radiation attenuation capability, so the support of the stimulable phosphor sheet has a specific gravity of 1.0 g / cm. ³ It is preferably formed of the above compound material or composite material, and the specific gravity is 1.5 g / cm. ³ Or more, 23 g / cm ³ It is particularly preferable to form the following compound material or composite material.
[0042]
In a preferred embodiment of the present invention, ten or more stimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0043]
In a further preferred embodiment of the present invention, 50 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0044]
In a further preferred embodiment of the present invention, 100 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0045]
In a further preferred embodiment of the present invention, 500 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0046]
In a further preferred embodiment of the present invention, 1000 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0047]
In a further preferred embodiment of the present invention, 5000 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0048]
In a further preferred embodiment of the present invention, 10,000 or more photostimulable phosphor layer regions are formed on the support of the stimulable phosphor sheet.
[0049]
In a more preferred embodiment of the present invention, the stimulable phosphor layer region of 50000 or more is formed on the support of the stimulable phosphor sheet.
[0050]
In a further preferred embodiment of the present invention, the stimulable phosphor layer region of 100,000 or more is formed on the support of the stimulable phosphor sheet.
[0051]
In a preferred embodiment of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet to a size of less than 5 square millimeters.
[0052]
In a further preferred embodiment of the present invention, each of the plurality of photostimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet to a size of less than 1 square millimeter.
[0053]
In a further preferred embodiment of the present invention, each of the plurality of photostimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet to a size of less than 0.5 square millimeters.
[0054]
In a further preferred embodiment of the present invention, each of the plurality of stimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.1 mm 2.
[0055]
In a further preferred embodiment of the present invention, each of the plurality of photostimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet to a size of less than 0.05 square millimeters.
[0056]
In a further preferred embodiment of the present invention, each of the plurality of photostimulable phosphor layer regions is formed on the support of the stimulable phosphor sheet in a size of less than 0.01 mm 2.
[0057]
In the present invention, the density of the stimulable phosphor layer region formed on the stimulable phosphor sheet is determined by the type of material of the support, the type of electron beam emitted from the radioactive labeling substance, and the like.
[0058]
In a preferred embodiment of the present invention, the stimulable phosphor sheet has the plurality of stimulable phosphor layer regions formed at a density of 10 / cm 2 or more.
[0059]
In a further preferred embodiment of the present invention, the stimulable phosphor sheet is formed with the plurality of stimulable phosphor layer regions at a density of 50 or more per square centimeter.
[0060]
In a further preferred embodiment of the present invention, the plurality of stimulable phosphor layer regions are formed on the stimulable phosphor sheet at a density of 100 / cm 2 or more.
[0061]
In a further preferred aspect of the present invention, the stimulable phosphor sheet is formed with the plurality of stimulable phosphor layer regions at a density of 500 or more per square centimeter.
[0062]
In a further preferred embodiment of the present invention, the plurality of stimulable phosphor layer regions are formed on the stimulable phosphor sheet at a density of 1000 / cm 2 or more.
[0063]
In a more preferred embodiment of the present invention, the stimulable phosphor sheet is formed with the plurality of photostimulable phosphor layer regions at a density of 5000 or more per square centimeter.
[0064]
In a further preferred embodiment of the present invention, the stimulable phosphor sheet is formed with the plurality of stimulable phosphor layer regions at a density of 10,000 or more per square centimeter.
[0065]
In a preferred embodiment of the present invention, the plurality of photostimulable phosphor layer regions are formed in a regular pattern on the support of the stimulable phosphor sheet.
[0066]
According to a preferred embodiment of the present invention, a plurality of photostimulable phosphor layer regions are formed in a regular pattern on the support of the stimulable phosphor sheet, and thus on the surface of a carrier such as a membrane filter. By forming a spot-like region containing a specific binding substance in the same regular pattern, only the corresponding photostimulable phosphor layer region is exposed by the radiolabeled substance contained in each spot-like region. Therefore, it is possible to generate data for biochemical analysis with high resolution and excellent quantitativeness.
[0067]
In the present invention, the photostimulable phosphor contained in the photostimulable phosphor layer is capable of storing radiation energy, excited by electromagnetic waves, and capable of emitting the stored radiation energy in the form of light. There is no particular limitation, and those that can be excited by light in the visible wavelength region are preferable. Specifically, for example, alkaline earth metal fluoride halide phosphors (Ba1-xM) disclosed in US Pat. No. 4,239,968 are disclosed. ²⁺ x) FX: yA (where M ²⁺ Is at least one alkaline earth metal element selected from the group consisting of Mg, Ca, Sr, Zn and Cd, X is at least one halogen selected from the group consisting of Cl, Br and I, A is Eu, Tb, Ce , Tm, Dy, Pr, Ho, Nd, Yb and Er, at least one trivalent metal element, x is 0 ≦ x ≦ 0.6, and y is 0 ≦ y ≦ 0.2. ), Alkaline earth metal fluoride halide phosphor SrFX: Z disclosed in JP-A-2-276997, wherein X is at least one halogen selected from the group consisting of Cl, Br and I, Z Is Eu or Ce.), Europium-activated composite halide phosphor BaFX · xNaX ′: aEu disclosed in JP-A-59-56479 ²⁺ (Here, X and X ′ are both at least one halogen selected from the group consisting of Cl, Br and I, x is 0 <x ≦ 2, and a is 0 <a ≦ 0.2. ), MOX: xCe which is a cerium-activated trivalent metal oxyhalide phosphor disclosed in JP-A-58-69281 (where M is Pr, Nd, Pm, Sm, Eu, Tb, Dy) At least one trivalent metal element selected from the group consisting of Ho, Er, Tm, Yb and Bi, X is one or both of Br and I, and x is 0 <x <0.1.) LnOX: xCe which is a cerium activated rare earth oxyhalide phosphor disclosed in US Pat. No. 4,539,137 (where Ln is at least one selected from the group consisting of Y, La, Gd and Lu) Rare earth element X Is at least one halogen selected from the group consisting of Cl, Br and I, x is 0 <x ≦ 0.1) and europium activated composite halogens disclosed in US Pat. No. 4,962,047 Physical phosphor M ^II FX ・ aM ^I X '・ bM' ^II X ^'' 2 · cM ^III X ^''' 3 xA: yEu ²⁺ (Here, M ^II Is at least one alkaline earth metal element selected from the group consisting of Ba, Sr and Ca, M ^I Is at least one alkali metal element selected from the group consisting of Li, Na, K, Rb and Cs, M ′ ^II Is at least one divalent metal element selected from the group consisting of Be and Mg, M ^III Is at least one trivalent metal element selected from the group consisting of Al, Ga, In and Tl, A is at least one metal oxide, X is at least one halogen selected from the group consisting of Cl, Br and I, X ', X ^'' And X ^''' Is at least one halogen selected from the group consisting of F, Cl, Br and I, a is 0 ≦ a ≦ 2, b is 0 ≦ b ≦ 10 ^-2 , C is 0 ≦ c ≦ 10 ^-2 And a + b + c ≧ 10 ^-2 Where x is 0 <x ≦ 0.5 and y is 0 <y ≦ 0.2. ) Can be preferably used.
[0068]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
[0069]
FIG. 1 is a schematic perspective view of a biochemical analysis unit.
[0070]
As shown in FIG. 1, the biochemical analysis unit 1 includes an adsorptive substrate 2 formed of nylon 6, and a solution containing a specific binding substance, for example, cDNA, is present on the surface of the adsorptive substrate 2. A large number of substantially circular spot-like regions 3 which are regularly dropped at regular intervals and contain a specific binding substance are formed on the adsorptive substrate 2.
[0071]
Although not exactly shown in FIG. 1, in the present embodiment, the substantially circular spot-like region 3 having a size of about 0.07 square millimeters is formed in an adsorbent substrate in a matrix of 120 columns × 160 rows. 2 is formed regularly, and therefore a total of 19200 spot-like regions 3 are formed.
[0072]
FIG. 2 is a schematic front view of the spotting device.
[0073]
In biochemical analysis, as shown in FIG. 2, for example, a solution containing a plurality of different cDNAs with known base sequences as specific binding substances is adsorbed on the adsorptive substrate 2 of the biochemical analysis unit 1. The spotting device 5 is dropped to form a large number of spot-like regions 3.
[0074]
As shown in FIG. 2, the spotting device 5 includes an injector 6 and a CCD camera 7 that inject a solution of a specific binding substance toward the biochemical analysis unit 1. While observing the tip and the region of the adsorbent substrate 2 where the solution containing a specific binding substance such as cDNA should be dropped, the adsorbent substrate where the tip of the injector 6 and the solution of the specific binding substance should be dropped When the center of the region 2 matches, the specific binding substance is dropped from the injector 6, and the solution of the specific binding substance is applied to the adsorptive substrate 2 of the biochemical analysis unit 1. It is guaranteed that it can be accurately dropped to form as many spot-like regions 3 as desired.
[0075]
FIG. 3 is a schematic longitudinal sectional view of the hybridization reaction container.
[0076]
As shown in FIG. 3, the hybridization reaction container 8 has a rectangular cross section, and accommodates therein a hybridization reaction solution 9 containing a biological substance that is a probe labeled with a labeling substance.
[0077]
In this embodiment, a hybridization reaction solution 9 containing a biologically-derived substance labeled with a radioactive labeling substance is prepared and accommodated in the hybridization reaction container 8.
[0078]
In hybridization, a specific binding substance such as cDNA is regularly dropped on the surface of the adsorptive substrate 2 to form a biochemical analysis unit 1 in which a large number of spot-like regions 3 are formed. Inserted inside.
[0079]
As a result, a specific binding substance such as cDNA contained in a large number of spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1 is labeled with a radiolabeling substance, and a hybridization reaction solution The substance derived from the living body contained in is selectively hybridized.
[0080]
In this way, radiation data of the radiolabeled substance is recorded in a large number of spot-like regions 3 of the biochemical analysis unit 1.
[0081]
The radiation data of the radiolabeled substance recorded in the many spot-like regions 3 of the biochemical analysis unit 1 is transferred to the photostimulable phosphor layer of the stimulable phosphor sheet, read by a scanner described later, and Data for chemical analysis is generated.
[0082]
FIG. 4 is a schematic perspective view of the stimulable phosphor sheet according to a preferred embodiment of the present invention.
[0083]
As shown in FIG. 4, the stimulable phosphor sheet 10 according to the present embodiment is formed of stainless steel, and a support body 11 in which a large number of recesses 13 and recesses 14 are regularly formed, and a support body 11. A number of photostimulable phosphor layer regions 12 formed by embedding photostimulable phosphors in a number of formed recesses 13, and a support 11 between a number of photostimulable phosphor layer regions 12. A number of additional photostimulable phosphor layer regions 15 formed by embedding photostimulable phosphors in a number of formed recesses 14 are provided.
[0084]
In this embodiment, the area of the recess 14 for forming each of the many additional photostimulable phosphor layer regions 15 is the same as the recess 13 for forming each of the many photostimulable phosphor layer regions 12. Therefore, the large number of additional photostimulable phosphor layer regions 15 are formed so as to have a smaller area than the number of photostimulable phosphor layer regions 12, respectively.
[0085]
Further, in the present embodiment, the stimulable phosphor is embedded in a number of the recesses 13 so that the surface of the stimulable phosphor layer region 14 coincides with the surface of the support 11, and additional stimuli are obtained. The photostimulable phosphor is embedded in a number of recesses 14 so that the surface of the phosphor layer region 15 coincides with the surface of the support 11.
[0086]
Here, the multiple recesses 13 are the same regular pattern as the multiple spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1, and the same size as the multiple spot-like regions 3. Thus, the support 11 is formed in a substantially circular shape.
[0087]
Therefore, although not accurately shown in FIG. 4, a substantially circular recess 13 having a size of about 0.07 square millimeters is formed in a number of spots formed on the adsorptive substrate 2 of the biochemical analysis unit 1. The same regular pattern as the region 3 is formed on the support 11 in a matrix of 120 columns × 160 rows, and a total of 19200 recesses 13 are formed in dots on the support 11 of the stimulable phosphor sheet 10. Has been.
[0088]
As a result, each stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 is only in the corresponding spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. The stimulable phosphor sheet 10 is overlapped with the biochemical analysis unit 1 so as to face each other, and the stimulable phosphor sheet 10 is contained by the radiolabeled substance contained in the spot-like region 3 of the biochemical analysis unit 1. The photostimulable phosphor regions 12 facing each other can be exposed.
[0089]
FIG. 5 shows a number of substances formed on the support 11 of the stimulable phosphor sheet 10 by radiolabeled substances contained in a number of spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. It is a schematic sectional drawing which shows the method of exposing the photostimulable phosphor layer area | region 12 of this.
[0090]
As shown in FIG. 5, the support 11 of the stimulable phosphor sheet 10 is formed on the support 11 of the stimulable phosphor sheet 10 by the radiolabeled substance contained in the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. In exposing a large number of photostimulable phosphor layer regions 12 formed, photostimulable phosphors are formed in a large number of recesses 13 formed on the support 11 of the stimulable phosphor sheet 10. Thus, the stimulable phosphor sheet 10 is grown so that the large number of photostimulable phosphor layer regions 12 face the large number of spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. Superposed on the chemical analysis unit 1.
[0091]
During the exposure, an electron beam (β-ray) is emitted from the radiolabeled substance contained in a large number of spot-like regions 3 of the biochemical analysis unit 1. The region 12 is formed on the support 11 in the same regular pattern as the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. Since the stimulable phosphor sheet 10 is overlaid on the biochemical analysis unit 1 so as to face the corresponding spot-like region 3 formed on the substrate 2, it is attached to the adsorptive substrate 2 of the biochemical analysis unit 1. Electron beams (β rays) emitted from the radiolabeled substances contained in the formed spot-like regions 3 are incident only on the corresponding stimulable phosphor layer regions 12, respectively, and the support 11 emits radiation. Virtually transparent Therefore, the electron beam (β-ray) is also prevented from being scattered within the support 11 of the stimulable phosphor sheet 10 and thus formed on the substrate 2 of the biochemical analysis unit 1. Only the corresponding stimulable phosphor layer region 12 can be selectively exposed by the radiolabeled substance contained in each spot-shaped region 3, while the stimulable phosphor sheet 10. Electron beams (β) emitted from the radiolabeled substances contained in the many spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 are added to the many additional photostimulable phosphor layer regions 15. A large number of additional photostimulable phosphor layer regions 15 are exposed by the radioactive labeling substance contained in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. To be effective Can be prevented.
[0092]
However, since it is extremely difficult to completely wash the radiolabeled substance adhering to the surface of the biochemical analysis unit 1 on which the spot-like region 3 is not formed at the time of hybridization, the spot-like region 3 is formed. At the time of hybridization, the attached radiolabeled substance remains on the surface of the biochemical analysis unit 1 that has not been washed, and the electron beam (β-ray) emitted from the remaining radiolabeled substance is accumulated fluorescent. It is unavoidable to enter a large number of photostimulable phosphor layer regions 12 formed on the support 11 of the body sheet 10, and many environmental radiations are also formed on the support 11 of the stimulable phosphor sheet 10. Is formed on the support 11 of the stimulable phosphor sheet 10 and is formed on the substrate 2 of the biochemical analysis unit 1. A number of photostimulable phosphor layer regions 12 exposed by the radiolabeled substances contained in a number of spot-like regions 3 are scanned with excitation light and emitted from a number of photostimulable phosphor layer regions 12. The data for biochemical analysis generated by photoelectrically detecting the photostimulated light adheres to the surface of the biochemical analysis unit 1 where the spot-like region 3 is not formed during the hybridization, and after washing In addition, electron beams (β rays) emitted from the remaining radiolabeled substance, environmental radiation, and the like are applied to the many photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10. Background noise due to incidence is inevitably included.
[0093]
On the other hand, in the many additional photostimulable phosphor layer regions 15 of the stimulable phosphor sheet 10, the radioactive labels contained in the many spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1. The electron beam (β-ray) emitted from the substance does not enter, and the electron beam emitted from the radiolabeled substance contained in the many spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 ( Since the β-rays) prevent the exposure of the many additional photostimulable phosphor layer regions 15 of the stimulable phosphor sheet 10, the many additional photostimulability of the stimulable phosphor sheet 10. In the phosphor layer region 15, electrons that are attached to the surface of the biochemical analysis unit 1 in which the spot-like region 3 is not formed during hybridization are released from the radiolabeled substance remaining after washing. Line (β line) A large number of additional photostimulable phosphor layer regions 15 of the stimulable phosphor sheet 10 are incident on the surface of the biochemical analysis unit 1 where the spot-like regions 3 are not formed upon hybridization. It is exposed only to electron beams (β rays) emitted from radioactively labeled substances remaining after cleaning and environmental radiation. Accordingly, a number of additional photostimulable phosphor layer regions 15 of the stimulable phosphor sheet 10 are scanned with excitation light, and the photostimulated light emitted from the number of additional photostimulable phosphor layers 15 is photoelectrically detected. Data generated by detection corresponds to background noise.
[0094]
In this way, radiation data of the radiolabeled substance is recorded in a number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10.
[0095]
FIG. 6 shows the present invention in which radiation data of a radiolabeled substance recorded in a number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is read to generate digital data. FIG. 7 is a schematic perspective view of a scanner according to a preferred embodiment, and FIG. 7 is a schematic perspective view showing details in the vicinity of the photomultiplier of the scanner.
[0096]
The scanner shown in FIG. 6 includes radiation data of a radiolabeled substance recorded on a number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 and a gel support or transfer support. A first laser excitation light source 21 that emits laser light 24 having a wavelength of 640 nm, and is configured to read data for biochemical analysis of a sample labeled with a fluorescent substance such as a fluorescent dye recorded in A second laser excitation light source 22 that emits a laser beam 24 having a wavelength of 532 nm and a third laser excitation light source 23 that emits a laser beam 24 having a wavelength of 473 nm are provided.
[0097]
In the present embodiment, the first laser excitation light source 21 is constituted by a semiconductor laser light source, and the second laser excitation light source 22 and the third laser excitation light source 23 are second harmonic generation elements. It is constituted by.
[0098]
The laser beam 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. The first dichroic mirrors 27 and 532 nm that transmit the laser light 4 of 640 nm and reflect the light having a wavelength of 532 nm in the optical path of the laser light 24 emitted from the first laser excitation light source 21 and reflected by the mirror 26. The second dichroic mirror 28 that transmits the light having the above wavelength and reflects the light having the wavelength of 473 nm is provided, and the laser light 24 generated by the first laser excitation light source 21 is the first dichroic mirror. 27 and the second dichroic mirror 28 are incident on the mirror 29.
[0099]
On the other hand, the laser light 24 generated from the second laser excitation light source 22 is collimated by the collimator lens 30 and then reflected by the first dichroic mirror 27, and its direction is changed by 90 degrees. The light passes through the second dichroic mirror 28 and enters the mirror 29.
[0100]
After the laser light 24 generated from the third laser excitation light source 23 is converted into parallel light by the collimator lens 31, it is reflected by the second dichroic mirror 28, and its direction is changed by 90 degrees. , Enters the mirror 29.
[0101]
The laser beam 24 incident on the mirror 29 is reflected by the mirror 29 and further incident on the mirror 32 and reflected.
[0102]
In the optical path of the laser beam 24 reflected by the mirror 32, a perforated mirror 34 formed by a concave mirror in which a hole 33 is formed in the center is arranged, and the laser beam 24 reflected by the mirror 32 is The light passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.
[0103]
The laser beam 24 incident on the concave mirror 38 is reflected by the concave mirror 38 and enters the optical head 35.
[0104]
The optical head 35 includes a mirror 36 and an aspheric lens 37, and the laser light 24 incident on the optical head 35 is reflected by the mirror 36 and is applied onto the glass plate 41 of the stage 40 by the aspheric lens 37. The light is incident on the stimulable phosphor sheet 10 or the gel support or transfer support.
[0105]
When the laser beam 24 is incident on the photostimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10, the photostimulable phosphor contained in the photostimulable phosphor layer region 12 is changed. When excited, the stimulating light 45 is emitted, and when the laser light 24 is incident on the gel support or the transfer support, a fluorescent substance such as a fluorescent dye is excited and the fluorescence 45 is emitted.
[0106]
The stimulating light 45 emitted from the stimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 or the fluorescence 45 emitted from the gel support or transfer support is transferred to the optical head 35. The aspherical lens 37 provided condenses on the mirror 36, is reflected by the mirror 36 on the same side as the optical path of the laser beam 24, becomes parallel light, and enters the concave mirror 38.
[0107]
The stimulated light 45 or fluorescence 45 incident on the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.
[0108]
As shown in FIG. 7, the stimulated light 45 or the fluorescent light 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by the concave mirror and incident on the filter unit 48, so that a predetermined value is obtained. Is cut off, enters the photomultiplier 50, and is detected photoelectrically.
[0109]
As shown in FIG. 7, the filter unit 48 includes four filter members 51a, 51b, 51c, and 51d. The filter unit 48 is moved in the left-right direction in FIG. 7 by a motor (not shown). It is configured to be possible.
[0110]
FIG. 8 is a schematic cross-sectional view along the line AA in FIG.
[0111]
As shown in FIG. 8, the filter member 51 a includes a filter 52 a, and the filter 52 a uses the first laser excitation light source 21 and is a fluorescent substance such as a fluorescent dye contained in the gel support or transfer support. Is a filter member used when reading fluorescence, and has a property of cutting light having a wavelength of 640 nm and transmitting light having a wavelength longer than 640 nm.
[0112]
FIG. 9 is a cross-sectional view taken along line BB in FIG.
[0113]
As shown in FIG. 9, the filter member 51 b includes a filter 52 b, and the filter 52 b uses the second laser excitation light source 22, and a fluorescent substance such as a fluorescent dye contained in the gel support or the transfer support. Is a filter member used when reading fluorescence, and has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm.
[0114]
FIG. 10 is a cross-sectional view taken along the line CC of FIG.
[0115]
As shown in FIG. 10, the filter member 51 c includes a filter 52 c, and the filter 52 c uses a third laser excitation light source 23, and a fluorescent material such as a fluorescent dye contained in the gel support or transfer support. Is a filter member used when reading fluorescence, and has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.
[0116]
11 is a cross-sectional view taken along the line DD of FIG.
[0117]
As shown in FIG. 11, the filter member 51 d includes a filter 52 d, and the filter 52 d uses the first laser excitation light source 21 to form a number of photostimulable phosphor layers formed on the stimulable phosphor sheet 10. This is a filter that is used when exciting the region 12 and reading the photostimulated light emitted from the photostimulable phosphor layer region 12, and the wavelength range of the photostimulated light emitted from the photostimulable phosphor layer region 12 The light has a property of transmitting only light of 640 nm and cutting light having a wavelength of 640 nm.
[0118]
Therefore, by selectively positioning the filter members 51a, 51b, 51c, and 51d on the front surface of the photomultiplier 50 according to the laser excitation light source to be used, the photomultiplier 50 photoelectrically converts only the light to be detected. Can be detected automatically.
[0119]
Analog data detected and generated photoelectrically by the photomultiplier 50 is converted to digital data by the A / D converter 53 with a scale factor suitable for the signal fluctuation range, and is input to the line buffer 54. .
[0120]
The line buffer 54 temporarily stores digital data for one main scanning line. When digital data for one main scanning line is stored as described above, the digital data is The data is output to a transmission buffer 55 having a capacity larger than that of the line buffer 54, and the transmission buffer 55 is configured to output the digital data to the data processing device 56 when digital data of a predetermined capacity is stored. Has been.
[0121]
Although not shown in FIG. 6, the optical head 35 is configured to be movable by a scanning mechanism in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y in FIG. All the photostimulable phosphor layer regions 12 formed on the support 11 of the fluorescent phosphor sheet 10 or the entire surface of the gel support or transfer support are scanned by the laser beam 24.
[0122]
FIG. 12 is a schematic plan view of the scanning mechanism of the optical head 35.
[0123]
In FIG. 12, for simplification, the optical system excluding the optical head 35 and the optical path of the laser beam 24 and the stimulating light 45 or the fluorescence 45 are omitted.
[0124]
As shown in FIG. 12, the scanning mechanism that scans the optical head 35 includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62 and 62 are fixed on the substrate 60. Further, a movable substrate 63 is provided in the sub-scanning direction indicated by the arrow Y in FIG.
[0125]
A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is engaged in the hole. ing.
[0126]
A main scanning pulse motor 65 is provided on the movable substrate 63, and the main scanning pulse motor 65 is configured to be able to drive an endless belt 66. The optical head 35 is fixed to the endless belt 66. When the endless belt 66 is driven by the main scanning pulse motor 65, the optical head 35 moves in the main scanning direction indicated by the arrow X in FIG. It is configured to be.
[0127]
In FIG. 12, 67 is a linear encoder that detects the position of the optical head 35 in the main scanning direction, and 68 is a slit of the linear encoder 67.
[0128]
Therefore, the endless belt 66 is driven in the main scanning direction by the main scanning pulse motor 65, and the movable substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61. 12 is moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y in FIG. 12, and is formed on the support 11 of the stimulable phosphor sheet 10 by the laser beam 24. The photostimulable phosphor layer region 12 or the entire surface of the gel support or transfer support is scanned.
[0129]
FIG. 13 is a block diagram showing a control system, an input system, and a drive system of the scanner shown in FIG.
[0130]
As shown in FIG. 13, the scanner control system includes a control unit 70 that controls the entire scanner, and the scanner input system is operated by an operator, and a keyboard 71 capable of inputting various instruction signals. It has.
[0131]
As shown in FIG. 13, the scanner drive system includes a main scanning pulse motor 65 that moves the optical head 35 in the main scanning direction, a sub scanning pulse motor 61 that moves the optical head 35 in the sub scanning direction, and four scanning motors. A filter unit motor 72 that moves the filter unit 48 including the filter members 51a, 51b, 51c, and 51d is provided.
[0132]
The control unit 70 can selectively output a drive signal to the first laser excitation light source 21, the second laser excitation light source 22 or the third laser excitation light source 23 and can output a drive signal to the filter unit motor 72. It is configured.
[0133]
FIG. 14 is a block diagram of the data processing device 56.
[0134]
As shown in FIG. 14, the data processing device 56 receives digital data temporarily stored in the transmission buffer 14, and temporarily stores it in a data temporary storage unit 75 and stores it in the data temporary storage unit 75. The correction data generation unit 76 that generates background noise correction data based on the digital data that has been read, and the digital data stored in the data temporary storage unit 75 are read out, and the background noise correction generated by the correction data generation unit 76 A data processing unit 77 that performs predetermined data processing on the digital data such as performing background noise correction according to the data, and a data storage unit 78 that stores digital data that has been subjected to data processing by the data processing unit 77 are provided. ing.
[0135]
The scanner configured as described above has a large number of bright spots due to the radioactive labeling substances contained in the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1 as follows. The stimulable phosphor layer region 12 is exposed, and the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 10 is read to generate biochemical analysis data.
[0136]
First, the stimulable phosphor sheet 10 is placed on the glass plate 41 of the stage 40 by the user.
[0137]
Next, an instruction signal to the effect that the radiation data recorded in the many photostimulable phosphor layer regions 12 formed on the stimulable phosphor sheet 10 is to be read is input to the keyboard 71 by the user.
[0138]
The instruction signal input to the keyboard 71 is input to the control unit 70. When the instruction signal is received, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal to move the filter unit 48. The filter member 51d including the filter 52d having the property of transmitting only the light in the wavelength region of the stimulating light emitted from the stimulable phosphor and cutting the light having the wavelength of 640 nm is used as the optical path of the stimulating light 45. Located within.
[0139]
Next, the control unit 70 outputs a drive signal to the first laser excitation light source 21, activates the first laser excitation light source 21, and emits laser light 24 having a wavelength of 640 nm.
[0140]
The laser light 24 emitted from the first laser excitation light source 21 is collimated by the collimator lens 25 and then enters the mirror 26 and is reflected.
[0141]
The laser beam 24 reflected by the mirror 26 passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.
[0142]
The laser beam 24 incident on the mirror 29 is reflected by the mirror 29 and further incident on the mirror 32 and reflected.
[0143]
The laser beam 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.
[0144]
The laser beam 24 incident on the concave mirror 38 is reflected by the concave mirror 38 and enters the optical head 35.
[0145]
The laser beam 24 incident on the optical head 35 is reflected by the mirror 36 and is formed on the support 11 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41 by the aspheric lens 37. The fluorescent material layer 12 is focused.
[0146]
In the present embodiment, a number of photostimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 are formed on a support 11 made of stainless steel having a property of attenuating radiation energy and spaced apart from each other. Therefore, the laser light 24 incident on the stimulable phosphor layer region 12 is scattered to excite the stimulable phosphor contained in the adjacent stimulable phosphor layer region 12. It becomes possible to prevent effectively.
[0147]
When the laser beam 24 enters the photostimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10, the photostimulable phosphor contained in the photostimulable phosphor layer region 12 is obtained. When excited by the laser beam 24, the photostimulable light 45 is emitted from the photostimulable phosphor.
[0148]
The stimulating light 45 emitted from the stimulable phosphor included in the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is collected by an aspheric lens 37 provided in the optical head 35. The light is reflected by the mirror 36 on the same side as the optical path of the laser light 24, becomes parallel light, and enters the concave mirror 38.
[0149]
The laser beam 24 incident on the concave mirror 38 is reflected by the concave mirror 38 and enters the perforated mirror 34.
[0150]
The stimulated light 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror and incident on the filter 52d of the filter unit 48 as shown in FIG.
[0151]
Since the filter 52d has a property of transmitting only light in the wavelength region of the stimulating light emitted from the stimulable phosphor and cutting light having a wavelength of 640 nm, the filter 52d has a wavelength of 640 nm which is excitation light. The light is cut, and only the light in the wavelength range of the stimulating light passes through the filter 52d and is detected photoelectrically by the photomultiplier 50.
[0152]
As described above, the optical head 35 is moved on the substrate 62 by the main scanning pulse motor 65 provided on the substrate 62 in the main scanning direction indicated by the arrow X in FIG. 61, the substrate 62 is moved in the sub-scanning direction indicated by the arrow Y in FIG. 12, so that all the photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 are formed. The stimulated light 45 scanned by the laser light 24 and emitted from the stimulable phosphor contained in the stimulable phosphor layer region 12 of the stimulable phosphor sheet 10 is photoelectrically generated by the photomultiplier 50. By detecting the radiation data of the radiolabeled substance recorded in a number of photostimulable phosphor layer regions 12 of the stimulable phosphor sheet 10, the analog data for biochemical analysis is read. It is possible to generate the data.
[0153]
The stimulable phosphor sheet 10 has a large number of additional layers formed by embedding a stimulable phosphor in a large number of recesses 14 formed in the support 11 between a large number of stimulable phosphor layer regions 12. A plurality of additional photostimulable phosphor layer regions 15 of the biochemical analysis unit 1 in which a large number of spot-like regions 3 are not formed upon hybridization. Because it is exposed to electron beams (β rays) emitted from radioactive labeling substances that remain on the surface and remain after cleaning, or environmental radiation, and accumulates radiation energy, it is accumulated by laser light 24. When the phosphor sheet 10 is scanned, the photostimulable phosphors included in a number of additional photostimulable phosphor layer regions 15 are excited by the laser light 24 to emit photostimulated light 45, Numerous photostimulable phosphor layers In the same manner as the photostimulable light 45 emitted from the photostimulable phosphor contained in the region 12, it was emitted from the photostimulable phosphor contained in many additional photostimulable phosphor layer regions 15. The stimulated light 45 is also detected photoelectrically by the photomultiplier 50.
[0154]
Therefore, analog data generated by scanning all the photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 with the laser light 24 includes the stimulable phosphor sheet 10. Analog data generated by detecting photostimulated light 45 emitted from photostimulable phosphors included in a number of additional photostimulable phosphor layer regions 15 formed on the support 11 are also included. ing.
[0155]
Analog data detected and generated photoelectrically by the photomultiplier 50 is converted to digital data by the A / D converter 53 with a scale factor suitable for the signal fluctuation range, and is input to the line buffer 54. .
[0156]
When the digital data for one main scanning line is stored in the line buffer 54, the digital data is output to the transmission buffer 55 having a capacity larger than the capacity of the line buffer 54. When the volume of digital data is stored, the digital data is output to the data processor 56.
[0157]
The digital data output to the data processing device 56 is temporarily stored in the data temporary storage unit 75.
[0158]
The digital data temporarily stored in the temporary data storage unit 75 is output to the correction data generation unit 76 and also to the data processing unit 77.
[0159]
Here, as described above, a large number of additional photostimulable phosphor layer regions 15 formed on the support 11 of the stimulable phosphor sheet 10 have a large number of spot-like regions 3 formed upon hybridization. The biochemical analysis unit 1 is exposed only to the electron beam (β-ray) emitted from the radiolabeled substance that remains on the surface of the biochemical analysis unit 1 and remains after the cleaning. The substrate 2 is not exposed to electron beams (β rays) emitted from a radiolabeled substance selectively contained in a number of spot-like regions 3 formed on the substrate 2. Therefore, a number of additional photostimulable phosphor layer regions 15 formed on the support 11 of the stimulable phosphor sheet 10 are scanned with the laser light and emitted from the number of additional photostimulable phosphor layers 15. Since the digital data generated by photoelectrically detecting the photostimulated light 45 corresponds to background noise, the correction data generation unit 76 is based on the digital data input from the data temporary storage unit 75. Then, background noise correction data is generated from digital data generated by photoelectrically detecting the photostimulated light 45 emitted from a number of additional photostimulable phosphor layers 15 and output to the data processing unit 77. To do.
[0160]
The data processing unit 77 subtracts the background noise correction data input from the correction data generation unit 76 from the digital data input from the data temporary storage unit 75, corrects the background noise, and further acquires necessary data. The processing is executed to store the digital data after the data processing in the data storage unit 78 and erase the digital data stored in the data temporary storage unit 75.
[0161]
In this way, the background noise is corrected, and other data processing is performed as necessary. Based on the digital data stored in the data storage unit 78, quantitative analysis is performed as desired.
[0162]
On the other hand, when reading fluorescence data of fluorescent substances such as electrophoresis data of denatured DNA labeled with a fluorescent dye recorded on a gel support or transfer support to generate biochemical analysis data, the user first As a result, the gel support or transfer support on which the fluorescence data is recorded is set on the glass plate 41 of the stage 40.
[0163]
Next, when the type of the fluorescent material is input to the keyboard 71 by the user, the control unit 70 causes the first laser excitation light source 21, the second laser excitation light source 22, and the third laser excitation light source 23 to be selected. A laser excitation light source that emits a laser beam 24 having a wavelength capable of efficiently exciting the input fluorescent substance is selected, and the input fluorescent substance is selected from the three filter members 51a, 51b, and 51c. A filter member having a property of cutting light having a wavelength of the laser light 24 used for excitation and transmitting light having a wavelength longer than the wavelength of the laser light 24 used for exciting the fluorescent material is selected.
[0164]
Next, the entire surface of the gel support or transfer support is scanned by the laser beam 24, and the fluorescence 45 emitted from the fluorescent material is photoelectrically detected by the photomultiplier 50 to generate analog data. It is digitized by the / D converter and data for biochemical analysis is generated.
[0165]
According to the present embodiment, the support 11 of the stimulable phosphor sheet is obtained by the radiolabeled substance selectively contained in the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. When exposing a large number of photostimulable phosphor layer regions 12 formed in the above, electron beams (β rays) are generated from radiolabeled substances selectively contained in a large number of spot-like regions 3 of the biochemical analysis unit 1. Although many of the stimulable phosphor layer regions 12 of the stimulable phosphor sheet 10 are emitted in the same regular pattern as the many spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1. The stimulable phosphor sheet 10 is superimposed on the biochemical analysis unit 1 so as to face the corresponding spot-like regions 3 formed on the support 11 and formed on the substrate 2 of the biochemical analysis unit 1. Have been Electron beams (β rays) emitted from the radiolabeled substances contained in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1 are applied to the corresponding stimulable phosphor layer region 12. Since the support 11 of the biochemical analysis unit 1 is made of stainless steel that does not substantially transmit radiation, the electron beam (β-ray) is generated in the support 11 of the stimulable phosphor sheet 10. Scattering is also prevented, and therefore the electron beams (β rays) emitted from the radiolabeled substances contained in each of the spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 face each other. Only the photostimulable phosphor layer region 12 can be surely exposed. Therefore, the exposed many photostimulable phosphor layer regions 12 are scanned with excitation light, and a plurality of photostimulable phosphor layers are scanned. From region 12 By detecting the issued stimulated emission 45 photoelectrically, with high resolution, it is possible to generate the data for biochemical analysis with excellent quantitative properties.
[0166]
However, since it is extremely difficult to completely wash the radiolabeled substance adhering to the surface of the biochemical analysis unit 1 in which a large number of spot-like regions 3 are not formed at the time of hybridization, the stimulable phosphor sheet Even when a large number of photostimulable phosphor layer regions 12 are formed on 10 supports 11, they adhere to the surface of the biochemical analysis unit 1 where a large number of spot-like regions 3 are not formed during hybridization. The radiolabeled substance remains after washing, and the photostimulable phosphor layer region 12 formed on the support 11 of the stimulable phosphor sheet 10 is the electron beam (β-ray) emitted from the remaining radiolabeled substance. It is difficult to avoid the incident, and the environmental radiation is also incident on the stimulable phosphor layer region 12 formed on the stimulable phosphor sheet 10. A large number of bright spots exposed to a radiolabeled substance formed on the support 11 of the body sheet 10 and selectively contained in a number of spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. The digital data for biochemical analysis generated by scanning the stimulable phosphor layer region 12 with the laser beam 24 and photoelectrically detecting the stimulated light emitted from the many stimulable phosphor layer regions 12 Is an electron beam (β-ray) emitted from a radiolabeled substance that adheres to the surface of the biochemical analysis unit 1 where a large number of spot-like regions 3 are not formed during hybridization and remains after washing. In addition, background noise caused by the incident of environmental radiation and the like on a large number of photostimulable phosphor layer regions 12 formed on the support 11 of the stimulable phosphor sheet 10 is inevitably included. They are out.
[0167]
However, according to this embodiment, the radiolabeled substance contained in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1 is formed on the support 11 of the stimulable phosphor sheet 10. While the corresponding stimulable phosphor layer region 12 can be selectively exposed, a number of additional stimulable phosphor layer regions formed on the support 11 of the stimulable phosphor sheet 10 15, an electron beam (β-ray) emitted from a radiolabeled substance selectively contained in a large number of spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 is incident, and a large number of additions are made. The effective stimulable phosphor layer region 15 can be effectively prevented from being exposed, so that a number of additional stimulable phosphor layer regions formed on the support 11 of the stimulable phosphor sheet 10 can be obtained. 15 is exclusively hybridize. At the time of cleaning, the electron beam (β-ray) emitted from the radiolabeled substance remaining on the surface of the biochemical analysis unit 1 where many spot-like regions 3 are not formed and remaining after cleaning, A large number of additional photostimulable phosphor layer regions 15 formed on the support 11 of the stimulable phosphor sheet 10 upon incidence of radiation or the like have a large number of spot-like regions 3 not formed upon hybridization. It is exposed only to electron beams (β rays) emitted from the radiolabeled substance that remains on the surface of the chemical analysis unit 1 and remains after cleaning, and environmental radiation, and therefore has a number of additional stimuli. The phosphor layer region 15 is scanned with laser light, and the photostimulated light emitted from a number of additional photostimulable phosphor layers 15 formed on the support 11 of the stimulable phosphor sheet 10 is photoelectrically detected. This Digital data generated by will correspond to the background noise.
[0168]
Therefore, according to the present embodiment, the correction data generation unit 76 of the data processing device 56 is emitted from a number of additional photostimulable phosphor layers 15 formed on the support 11 of the stimulable phosphor sheet 10. Background noise correction data is generated from digital data generated by photoelectrically detecting the stimulated light 45, and the data processing unit 77 scans the entire surface of the stimulable phosphor sheet 10 with the laser light 24. Since the background noise is corrected by subtracting the background noise correction data generated by the correction data generation unit 76 from the generated digital data, the raw data from which the background noise has been removed is accurately obtained. It becomes possible to generate data for chemical analysis.
[0169]
Furthermore, according to this embodiment, a large number of additional stimulable phosphor layer regions 15 of the stimulable phosphor sheet 10 are regularly arranged on the support 11 between the many stimulable phosphor layer regions 12. Since the stimulable phosphor is embedded and formed in the many recessed portions 14 formed, even if the background noise varies depending on the position on the surface of the stimulable phosphor sheet 10, it is accurate. Data for biochemical analysis from which background noise is removed can be generated.
[0170]
FIG. 15 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.
[0171]
As shown in FIG. 15, the stimulable phosphor sheet 80 according to this embodiment includes a support body 81 formed of silicon nitride and a large number of through holes 83 formed in the support body 81 so as to be separated from each other. The photostimulable phosphors are embedded in the plurality of photostimulable phosphor layer regions 82 formed on the support 81 between the plurality of photostimulable phosphor layer regions 82 and 2 orthogonal to each other. A stripe-shaped additional photostimulable phosphor layer region 85 is formed by embedding photostimulable phosphor in the groove 84 of the book.
[0172]
Here, a large number of through-holes 83 are formed in the support 81 in the same regular pattern as the large number of spot-like regions 3 formed in the adsorptive substrate 2 of the biochemical analysis unit 1, and thus accumulated. When the fluorescent phosphor sheet 80 is superposed on the biochemical analysis unit 1, each of the stimulable phosphor layer regions 82 is a corresponding spot-like region formed on the adsorptive substrate 2 of the biochemical analysis unit 1. The stimulable phosphor sheet 80 is configured to face only 3.
[0173]
Also in this embodiment, the support 81 of the stimulable phosphor sheet 80 is formed by the radiolabeled substance selectively contained in the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. When exposing a large number of photostimulable phosphor layer regions 82 formed on the substrate, the same regular pattern as that of the large number of spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 is used. A large number of photostimulable phosphor layer regions 82 formed on the support 81 of the phosphor sheet 80 are respectively opposed to the corresponding spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1. The stimulable phosphor sheet 80 is superimposed on the biochemical analysis unit 1.
[0174]
Therefore, the electron beam (β-ray) emitted from the radiolabeled substance contained in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1 is emitted from the corresponding phosphor sheet 80. Since the support 81 of the stimulable phosphor sheet 80 is made of silicon nitride having the property of attenuating radiation energy, the electron beam (β-ray) is incident on the stimulable phosphor layer region 82 and is therefore stored in the stimulable phosphor layer 80. Scattering in the support 81 of the body sheet 80 is also prevented, so that the stimulable phosphor is contained by the radiolabeled substance contained in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. Only the corresponding photostimulable phosphor layer region 82 of the sheet 80 can be selectively exposed, and therefore, a large number of the exposed photostimulable phosphor layer regions 82 are scanned with excitation light. By detecting the photostimulated light 45 emitted from the photostimulable phosphor layer region 82 photoelectrically, it is possible to generate biochemical analysis data with high resolution and excellent quantification.
[0175]
On the other hand, a large number of spot-like shapes formed on the substrate 2 of the biochemical analysis unit 1 in the stripe-shaped additional stimulable phosphor layer region 85 formed on the support 81 of the stimulable phosphor sheet 80. It effectively prevents the electron beam (β-ray) emitted from the radiolabeling material selectively contained in the region 3 from being incident and exposing a large number of additional photostimulable phosphor layer regions 85. Therefore, a large number of spot-like regions 3 are formed exclusively in the stripe-shaped additional stimulable phosphor layer region 85 formed on the support 81 of the stimulable phosphor sheet 80 during the hybridization. Electron beam (β-rays) emitted from radioactive labeling substances that remain on the surface of the biochemical analysis unit 1 that has not been cleaned, and remain after cleaning, and incident radiation are incident on the strip. Phosphor layer The region 85 is attached to the surface of the biochemical analysis unit 1 where a large number of spot-like regions 3 are not formed during hybridization, and the electron beam (β-ray) emitted from the radiolabeled substance remaining after washing. ), Or by exposure to ambient radiation or the like. Therefore, the stripe-shaped additional photostimulable phosphor layer region 85 is scanned with a laser beam and emitted from the stripe-shaped additional photostimulable phosphor layer 85. Since the digital data generated by photoelectrically detecting the exhaust light corresponds to the background noise, it is emitted from the striped additional stimulable phosphor layer 85 in the same manner as in the above embodiment. The background noise correction data is generated from the digital data generated by photoelectrically detecting the stimulated light 45, and the accumulated fluorescence By subtracting the background noise correction data from the digital data generated by scanning the entire surface of the sheet 80 with the laser beam 24, the biochemical analysis data from which the background noise has been accurately removed can be generated. It becomes possible.
[0176]
FIG. 16 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.
[0177]
As shown in FIG. 16, the stimulable phosphor sheet 90 according to the present embodiment includes a support 91 formed of polyethylene terephthalate and a number of patterns formed on the surface of the support 91 in a regular pattern. On the surface of the support 91 between the photostimulable phosphor layer region 92 and the many photostimulable phosphor layer regions 92, a number of regularly formed additional photostimulable phosphor layer regions 95 are provided. I have.
[0178]
Here, a large number of photostimulable phosphor layer regions 92 have the same regular pattern as a large number of spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1, and a large number of spots. Is formed in a substantially circular shape on the surface of the support 91 with the same size as the region 3, and accordingly, when the stimulable phosphor sheet 90 and the biochemical analysis unit 1 are overlaid and exposed. The stimulable phosphor sheet 90 is configured such that each stimulable phosphor layer region 92 faces and contacts only the corresponding spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. Has been.
[0179]
According to the present embodiment, the support 91 of the stimulable phosphor sheet is obtained by the radiolabeling substance selectively contained in the many spot-like regions 3 formed on the adsorptive substrate 2 of the biochemical analysis unit 1. When exposing a large number of photostimulable phosphor layer regions 82 formed on the surface, a plurality of spot-like regions 3 formed on the substrate 2 of the biochemical analysis unit 1 have the same regular pattern. Each stimulable phosphor layer region 92 formed on the surface of the support 91 of the stimulable phosphor sheet 90 is only the corresponding spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. Since the stimulable phosphor sheet 90 and the biochemical analysis unit 1 are overlapped so as to face each other and come into contact with each other, they are included in each spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. From radioactively labeled substances Most of the emitted electron beams (β rays) are incident on the corresponding photostimulable phosphor layer region 12 and are therefore selectively included in the spot-like region 3 formed on the substrate 2 of the biochemical analysis unit 1. The radioactive labeling substance that has been used makes it possible to selectively expose the corresponding stimulable phosphor layer region 82 of the stimulable phosphor sheet 90, so that a large number of exposed stimulable phosphor layer regions The data for biochemical analysis with high resolution and excellent quantitativeness is obtained by scanning 92 with excitation light and photoelectrically detecting the photostimulated light 45 emitted from a number of photostimulable phosphor layer regions 92. Can be generated.
[0180]
On the other hand, a large number of additional stimulable phosphor layer regions 95 formed on the surface of the support 91 of the stimulable phosphor sheet 90 are formed on the substrate 2 of the biochemical analysis unit 1. Effectively preventing an electron beam (β-ray) emitted from the radiolabeled substance contained in the spot-like region 3 from entering and exposing a large number of additional photostimulable phosphor layer regions 95. Therefore, a large number of spot-like regions 3 are formed exclusively during the hybridization in the numerous additional photostimulable phosphor layer regions 95 formed on the surface of the support 91 of the stimulable phosphor sheet 90. Electron beam (β-rays) emitted from radioactive labeling substances that adhere to the surface of the substrate 2 of the biochemical analysis unit 1 that has not been cleaned and remain even after cleaning, and environmental radiation are incident to the fluorescent light. Of the support 91 of the body sheet 90 A large number of additional photostimulable phosphor layer regions 95 formed on the surface are attached to the surface of the substrate 2 of the biochemical analysis unit 1 where a large number of spot-like regions 3 are not formed during hybridization, It is exposed only by electron beams (β rays) emitted from the radiolabeled substance remaining after washing, environmental radiation, etc. Therefore, a number of additional photostimulable phosphor layer regions 95 are scanned with laser light. The digital data for biochemical analysis generated by photoelectrically detecting the photostimulated light emitted from a number of additional photostimulable phosphor layers 95 corresponds to background noise. Similar to the embodiment, from the digital data generated by photoelectrically detecting the photostimulated light 45 emitted from a number of additional photostimulable phosphor layers 95, the background Background noise correction data is generated, and the background noise correction data is subtracted from the digital data generated by scanning the entire surface of the stimulable phosphor sheet 90 with the laser beam 24, thereby accurately reducing the background noise. Data for the removed biochemical analysis can be generated.
[0181]
The present invention is not limited to the above-described embodiments, and various modifications are possible within the scope of the invention described in the claims, and these are also included in the scope of the present invention. Needless to say.
[0182]
For example, in the above-described embodiment, a plurality of different cDNAs having known base sequences are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to cDNA. It can specifically bind to biologically derived substances such as cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA, RNA, etc. Any specific binding substance whose base length, composition, etc. are known can be used as the specific binding substance of the present invention.
[0183]
Furthermore, in the above-described embodiment, the biological substance labeled with the radioactive labeling substance is hybridized to the specific binding substance, but it is not always necessary to hybridize the biological substance to the specific binding substance. It is not necessary, and a substance derived from a living body can be specifically bound to a specific binding substance by a reaction such as an antigen-antibody reaction or a receptor / ligand instead of hybridization.
[0184]
Further, in the above embodiment, the biochemical analysis unit 1 drops a solution containing a specific binding substance such as cDNA on the surface of the adsorptive substrate 2 and is labeled with a radioactive labeling substance. Is formed with a large number of spot-like regions 3 formed by hybridizing with a specific binding substance, and a plurality of through holes or recesses are formed in the substrate, and a plurality of through holes or recesses formed in the substrate are formed. The inside of the recess is filled with an adsorbent material such as nylon 6 to form a large number of adsorbable regions spaced apart from each other, and a solution containing a specific binding substance such as cDNA is dropped on the numerous adsorbent regions, A biochemical substance labeled with a radioactive labeling substance is selectively hybridized with specific binding substances contained in a large number of adsorptive regions to form a biochemical analysis unit 1. It may be.
[0185]
Further, in the embodiment shown in FIGS. 1 to 14, the support 11 of the stimulable phosphor sheet 10 is formed of stainless steel, and in the embodiment shown in FIG. 15, the stimulable phosphor sheet. In the embodiment shown in FIG. 16, the support 91 of the stimulable phosphor sheet 90 is made of polyethylene terephthalate, but the stimulable phosphor sheet is formed of silicon nitride. It is not always necessary to form the supports 11, 81, 91 of stainless steel, silicon nitride, or polyethylene terephthalate, and support of the stimulable phosphor sheets 10, 80, 90 by other materials. The bodies 11, 81, 91 can also be formed. The supports 11, 81, 91 of the stimulable phosphor sheets 10, 80, 90 are preferably formed of a material having a property of attenuating radiation energy, but the material is not particularly limited. The supports 11, 81, 91 of the stimulable phosphor sheets 10, 80, 90 can be formed of either an inorganic compound material or an organic compound material, but are particularly preferably made of a metal material, a ceramic material, or a plastic material. It is formed. Inorganic compound materials include, for example, gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin, selenium and other metals; brass, stainless steel, bronze alloys; silicon, Silicon materials such as amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide Can be mentioned. As the organic compound material, polymer compounds are preferably used. For example, polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon such as nylon 6, nylon 6,6, nylon 4,10; Polyimide; Polysulfone; Polyphenylene sulfide ; Silicon resin such as polydiphenylsiloxane; phenolic resin such as novolac; epoxy resin; polyurethane; Butadiene-styrene copolymer; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan; urushi; polyamide such as gelatin, collagen, keratin, and a copolymer of these polymer compounds A polymer etc. can be mentioned.
[0186]
In the above embodiment, the stimulable phosphor layer regions 12, 82, 92 of the stimulable phosphor sheets 10, 80, 90 are all formed on the adsorptive substrate 2 of the biochemical analysis unit 1. However, the stimulable phosphor layer regions 12, 82, 92 of the stimulable phosphor sheets 10, 80, 90 have a substantially circular shape. It is not necessarily required to be formed, and it may be formed in a shape other than a circle such as a substantially rectangular shape, and the stimulable phosphor layer regions 12, 82, and 82 of the stimulable phosphor sheets 10, 80, 90, respectively. It is not always necessary to form 92 so that the size thereof is equal to the size of the spot-like region 3 of the biochemical analysis unit 1.
[0187]
Furthermore, in the said embodiment, the substantially circular spot-like area | region 3 which has a size of about 0.07 square millimeter is regularly formed in the absorptive substrate 2 in the matrix form of 120 columns x 160 rows, therefore , A total of 19200 spot-like regions 3 are formed, and thus the support 11, 81, 91 of the stimulable phosphor sheet 10, 80, 90 has a total of 19200 having a size of about 0.07 square millimeters. The circular photostimulable phosphor layer regions 12, 82, and 92 are formed in a matrix of 120 columns × 160 rows, but the number and size of the spot-like regions 3 can be arbitrarily selected according to the purpose. Therefore, the number of stimulable phosphor layer regions 12, 82, 92 formed on the supports 11, 81, 91 of the stimulable phosphor sheets 10, 80, 90 is The size and size can be arbitrarily selected according to the purpose. Preferably, the spot-like region 3 having a size of 10 or more and less than 5 square millimeters is formed on the adsorptive substrate 2 of the biochemical analysis unit 1 at a density of 10 pieces / square centimeter or more. 80, 90 support 11, 81, 91 with stimulable phosphor layer regions 12, 82, 92 having a size of 10 or more and less than 5 square millimeters are formed at a density of 10 or more per square centimeter. The
[0188]
In the above embodiment, the stimulable phosphor layer regions 12, 82, and 92 of the stimulable phosphor sheets 10, 80, and 90 are formed on the adsorptive substrate 2 of the biochemical analysis unit 1. Although formed in the same regular pattern as the spot-like region 3, the stimulable phosphor layer regions 12, 82, and 92 of the stimulable phosphor sheets 10, 80, and 90 are the biochemical analysis unit 1. It is only necessary to form the same pattern as a large number of spot-like regions 3 formed on the adsorbent substrate 2, and a large number of spots are formed on the adsorbent substrate 2 of the biochemical analysis unit 1 in a regular pattern. It is not always necessary to form the region 3 or the stimulable phosphor layer regions 12, 82, 92 of the stimulable phosphor sheets 10, 80, 90 in a regular pattern.
[0189]
Furthermore, in the embodiment shown in FIGS. 1 to 14 and the embodiment shown in FIG. 16, multiple additions to the support 11, 91 between the multiple photostimulable phosphor layer regions 12, 92 are provided. However, forming a number of additional photostimulable phosphor layer regions between a number of photostimulable phosphor layer regions 12, 92 is not possible. It is not always necessary, and any number of additional photostimulable phosphor layer regions can be provided at any position on the supports 11 and 91 depending on the situation.
[0190]
In the embodiment shown in FIG. 15, the stimulable phosphor is embedded in two grooves 84 formed in the support 81 between the many stimulable phosphor layer regions 82 and perpendicular to each other. Thus, two stripe-shaped additional photostimulable phosphor layer regions 85 that are orthogonal to each other are formed, but it is always necessary that the stripe-shaped additional photostimulable phosphor layer regions are orthogonal to each other. The number can also be arbitrarily determined according to the situation.
[0191]
Further, in the embodiment shown in FIGS. 1 to 14 and the embodiment shown in FIG. 16, a number of additional photostimulable phosphor layer regions 15, 95 are formed on the support 11. Although formed in the recess 14 or on the surface of the support 91, instead of a number of additional stimulable phosphor layer regions 15, 95, as in the embodiment shown in FIG. A stripe-shaped additional photostimulable phosphor layer region may be formed in the recess 14 formed in the support 11 or on the surface of the support 91.
[0192]
Further, in the embodiment shown in FIG. 15, two mutually perpendicular stripe-like additional stimulable phosphor layer regions 85 are formed in the groove 84 of the support 81. A plurality of additional photostimulable phosphor layer regions similar to the embodiment shown in FIGS. 1 to 14 and FIG. May be formed in a large number of recesses formed on the support 81 or on the surface of the support 81.
[0193]
Further, in the embodiment shown in FIGS. 1 to 14 and the embodiment shown in FIG. 16, the number of additional stimulable phosphor layer regions 15, 95 has a stimulable phosphor area. A number of additional photostimulable phosphors are formed so as to be smaller than the area of the layer regions 12 and 92 but smaller than the area of the photostimulable phosphor layer regions 12 and 92. It is not always necessary to form the layer regions 15 and 95, and the areas of the many additional stimulable phosphor layer regions 15 and 95 can be arbitrarily selected depending on the situation.
[0194]
Further, in the embodiment shown in FIG. 1 to FIG. 14, the photostimulable phosphor is in a large number of recesses 13 so that the surface of the photostimulable phosphor layer region 12 coincides with the surface of the support 11. Embedded in the substrate, a number of photostimulable phosphor layer regions 12 are formed. However, a number of photostimulable phosphor layer regions 12 are formed so that the surface of the photostimulable phosphor layer region 12 coincides with the surface of the support 11. It is not always necessary to form the phosphor layer region 12, and the surface of the stimulable phosphor layer region 12 is located below or above the surface of the support 11. Also good.
[0195]
Further, in the embodiment shown in FIG. 15, the stripe-shaped additional stimulable phosphor layer region 85 is formed by filling the groove 84 formed in the support 81 with the stimulable phosphor. However, a slot may be formed in the support 81, and the stimulable phosphor may be embedded in the slot to form a stripe-shaped additional stimulable phosphor layer region 85.
[0196]
In addition, in the embodiment shown in FIGS. 1 to 14, a large number of additional photostimulable phosphor layer regions 15 are provided with a stimulable phosphor in a large number of recesses 14 formed in the support 11. However, in place of the recesses 14, a large number of through holes are formed in the support 11, and a stimulable phosphor is embedded in the large number of through holes to provide a large number of additional stimuli. A phosphor layer region can also be formed.
[0197]
【The invention's effect】
According to the present invention, a plurality of spot-like regions containing a specific binding substance that can specifically bind to a substance derived from a living body and has a known base sequence, base length, composition, and the like are provided on the surface of the carrier. A specific binding substance that is formed in high density and contained in multiple spot-like areas is specifically bound to a biologically-derived substance labeled with a radiolabeled substance to selectively label multiple spot-like areas. In this case, it is possible to generate biochemical analysis data with excellent resolution and high resolution, and a method for reading the data for biochemical analysis recorded on the storage phosphor sheet. It becomes possible to provide.
[Brief description of the drawings]
FIG. 1 is a schematic perspective view of a unit for biochemical analysis.
FIG. 2 is a schematic front view of a spotting device.
FIG. 3 is a schematic longitudinal sectional view of a hybridization reaction container.
FIG. 4 is a schematic perspective view of a stimulable phosphor sheet according to a preferred embodiment of the present invention.
FIG. 5 is a schematic diagram showing a large number of phosphorescent substance sheets formed on a support of a stimulable phosphor sheet by radiolabeled substances contained in a large number of spot-like regions formed on an adsorptive substrate of a biochemical analysis unit; It is a schematic sectional drawing which shows the method of exposing a photostimulable fluorescent substance layer area | region.
FIG. 6 is a scanner according to a preferred embodiment of the present invention for reading data for biochemical analysis recorded in a number of photostimulable phosphor layer regions formed on a support of a stimulable phosphor sheet. FIG.
7 is a schematic perspective view showing details in the vicinity of a photomultiplier of the scanner shown in FIG. 6. FIG.
FIG. 8 is a schematic cross-sectional view taken along the line AA in FIG.
FIG. 9 is a cross-sectional view taken along line BB in FIG.
FIG. 10 is a cross-sectional view taken along the line CC of FIG.
FIG. 11 is a cross-sectional view taken along the line DD of FIG. 7;
FIG. 12 is a schematic plan view of a scanning mechanism of the optical head.
FIG. 13 is a block diagram showing a control system, an input system, and a drive system of a scanner according to a preferred embodiment of the present invention.
FIG. 14 is a block diagram of a data processing apparatus.
FIG. 15 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.
FIG. 16 is a schematic perspective view of a stimulable phosphor sheet according to another preferred embodiment of the present invention.
[Explanation of symbols]
1 Biochemical analysis unit
2 Adsorbent substrate
3 Spot-like areas
5 Spotting device
6 Injector
7 CCD camera
8 Hybridization container
9 Hybridization solution
10 Storage phosphor sheet
11 Support
12 photostimulable phosphor layer region
13 recess
14 recess
15 Additional stimulable phosphor layer region
21 First laser excitation light source
22 Second laser excitation light source
23 Third laser excitation light source
24 Laser light
25 Collimator lens
26 Mirror
27 First dichroic mirror
28 Second dichroic mirror
29 Mirror
30 Collimator lens
31 Collimator lens
32 mirror
33 Hole in the mirror
34 Hole mirror
35 Optical head
36 mirror
37 Aspherical lenses
38 Concave mirror
40 stages
41 glass plate
45 Fluorescence or bright light
48 Filter unit
50 Photomultiplier
51a, 51b, 51c, 51d Filter member
52a, 52b, 52c, 52d Filter
53 A / D converter
54 Line buffer
55 Transmission buffer
56 Data processing equipment
60 substrates
61 Sub-scanning pulse motor
62 A pair of rails
63 Movable substrate
64 rods
65 Main scan pulse motor
66 Endless Belt
67 Linear encoder
68 Linear encoder slit
70 Control unit
71 keyboard
72 Filter unit motor
75 Data temporary storage
76 Correction data generator
77 Data processing section
78 Data storage
80 Storage phosphor sheet
81 Support
82 photostimulable phosphor layer region
83 holes
84 groove
85 Stripe-like additional stimulable phosphor layer region
90 Storage phosphor sheet
91 Support
92 photostimulable phosphor layer region
95 Additional stimulable phosphor layer region

Claims (28)

A plurality of photostimulable phosphor layer regions spaced apart from each other, and further spaced apart from the plurality of photostimulable phosphor layer regions. A stimulable phosphor sheet characterized in that a photostimulable phosphor layer region is formed. A plurality of holes are formed in the support so as to be separated from each other, and the photostimulable phosphor layer region is formed by filling the photostimulable phosphor in the plurality of holes. The stimulable phosphor sheet according to claim 1. A plurality of through holes are formed in the support so as to be spaced apart from each other, and the plurality of photostimulable phosphor layer regions are filled with the photostimulable phosphor in the plurality of through holes, 3. The stimulable phosphor sheet according to claim 2, wherein the stimulable phosphor sheet is formed. A plurality of recesses are formed on the support so as to be spaced apart from each other, and the plurality of stimulable phosphor layer regions are formed by filling the plurality of recesses with the stimulable phosphor. The stimulable phosphor sheet according to claim 2, wherein The stimulable phosphor sheet according to claim 1, wherein the plurality of photostimulable phosphor layer regions are formed on the support. The stimulable phosphor sheet according to any one of claims 1 to 5, wherein the plurality of photostimulable phosphor layer regions are formed in a dot shape on the support. The stimulable phosphor sheet according to any one of claims 1 to 6, wherein the plurality of additional photostimulable phosphor layer regions are formed in a dot shape on the support. The plurality of additional photostimulable phosphor layer regions are formed in a dot shape on the support between at least some of the plurality of photostimulable phosphor regions. The stimulable phosphor sheet described. The stimulable phosphor sheet according to any one of claims 1 to 6, wherein the at least one additional stimulable phosphor layer region is formed in a stripe shape on the support. . The at least one additional stimulable phosphor layer region is formed in a stripe shape on the support between at least a part of the plurality of photostimulable phosphor regions. 9. The stimulable phosphor sheet according to 9. The at least one additional photostimulable phosphor layer region is formed on the support so that the area thereof is smaller than the area of each of the plurality of photostimulable phosphor layer regions. The stimulable phosphor sheet according to any one of claims 1 to 10, characterized in that The stimulable phosphor sheet according to any one of claims 1 to 11, wherein the support is formed of a material that attenuates radiation. The support has a property of attenuating radiation energy to 1/5 or less when radiation passes through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. The stimulable phosphor sheet according to claim 12, which is made of a material. The support has a property of attenuating radiation energy to 1/10 or less when radiation passes through the support by a distance equal to the distance between adjacent stimulable phosphor layer regions. The stimulable phosphor sheet according to claim 13, which is made of a material. The support has a property of attenuating the energy of radiation to 1/100 or less when radiation passes through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. The stimulable phosphor sheet according to claim 14, which is made of a material. The stimulable phosphor sheet according to any one of claims 13 to 15, wherein the support is formed of a material selected from the group consisting of a metal material, a ceramic material, and a plastic material. A plurality of photostimulable phosphor layer regions spaced apart from each other, and further spaced apart from the plurality of photostimulable phosphor layer regions. Biologically-derived substance in which a stimulable phosphor sheet in which a stimulable phosphor layer region is formed and a specific binding substance having a known structure or characteristic are dropped, and the specific binding substance is labeled with a radioactive labeling substance Is selectively included in the plurality of spot-like regions by overlapping with the unit for biochemical analysis having a plurality of spot-like regions formed by selectively binding and selectively labeling. After exposing the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet with the radioactive labeling substance, the plurality of stimulable phosphor layer regions of the stimulable phosphor sheet and the at least one One additional Exciting phosphors included in the plurality of stimulable phosphor layer regions and the at least one additional stimulable phosphor layer region are irradiated with excitation light on the stimulable phosphor layer region Then, the photostimulated light emitted from the photostimulable phosphor is photoelectrically detected, the obtained analog data is digitized to generate digital data, and excited in the plurality of photostimulable phosphor layer regions. By irradiating light, the photostimulated light emitted from the photostimulable phosphor is photoelectrically detected, and excitation light is applied to the at least one additional photostimulable phosphor layer region from the obtained digital data. Irradiating and stimulating fluorescence emitted from the photostimulable phosphor, photoelectrically detected, and subtracting the obtained digital data to generate biochemical analysis data. A method for reading data for biochemical analysis recorded on body sheets. A plurality of holes are formed in the support so as to be spaced apart from each other, and the plurality of photostimulable phosphor layer regions are formed by filling the plurality of holes with a stimulable phosphor. The method for reading data for biochemical analysis recorded on the stimulable phosphor sheet according to claim 17. 18. The biochemical analysis data recorded on the stimulable phosphor sheet according to claim 17, wherein the plurality of photostimulable phosphor layer regions are formed on the surface of the support. Method. 21. The stimulable phosphor sheet according to claim 17, wherein the plurality of photostimulable phosphor layer regions are formed in dots on the support. How to read data for biochemical analysis. 21. The recording on the stimulable phosphor sheet according to claim 17, wherein a plurality of additional photostimulable phosphor layer regions are formed in a dot shape on the support. To read the data for biochemical analysis. The plurality of additional photostimulable phosphor layer regions are formed in a dot shape on the support between at least some of the plurality of photostimulable phosphor regions. A method for reading data for biochemical analysis recorded on the stimulable phosphor sheet described in 1. The stimulable phosphor sheet according to any one of claims 17 to 20, wherein the at least one additional stimulable phosphor layer region is formed in a stripe shape on the support. To read data for biochemical analysis recorded in The at least one additional stimulable phosphor layer region is formed in a stripe shape on the support between at least a part of the plurality of photostimulable phosphor regions. 23. A method for reading data for biochemical analysis recorded on the stimulable phosphor sheet according to 23. The at least one additional photostimulable phosphor layer region is formed on the support so that the area thereof is smaller than the area of each of the plurality of photostimulable phosphor layer regions. 25. A method for reading data for biochemical analysis recorded on a stimulable phosphor sheet according to any one of claims 17 to 24. The method for reading data for biochemical analysis recorded on a stimulable phosphor sheet according to any one of claims 17 to 25, wherein the support is formed of a material that attenuates radiation. . The support has a property of attenuating radiation energy to 1/5 or less when radiation passes through the support by a distance equal to the distance between adjacent photostimulable phosphor layer regions. 27. The method for reading data for biochemical analysis recorded on the stimulable phosphor sheet according to claim 26, wherein the biochemical analysis data is formed of a material. 28. The data for biochemical analysis recorded on the stimulable phosphor sheet according to claim 27, wherein the support is formed of a material selected from the group consisting of a metal material, a ceramic material, and a plastic material. How to read.

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