JP2003028878A - Unit for biochemical analysis and method for exposing photostimulable phosphor layer using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a unit for biochemical analysis capable of preventing noise caused by the scattering of electron radiation (beta rays) emitted from a radioactive labelling substance from being created in data for biochemical analysis even in the case of highly densely forming a plurality of spot-shaped regions containing a specifically combining substance in a surface of a carrier, obtaining the unit for biochemical analysis by selective labelling with the radioactive labelling substance, bringing the unit into close contact with the photostimulable phosphor layer, exposing the photostimulable phosphor layer with the radioactive labelling substance, irradiating the photostimulable phosphor layer with exciting light, photoelectrically detecting the emitted stimulated light emitted from the photostimulable phosphor layer, creating the data for biological analysis, and analyzing substances derived from an organism. SOLUTION: The unit 1 for biochemical analysis is provided with a plurality of adsorptive regions 4 formed of adsorptive materials at intervals with one another and a plurality of separating parts 2 formed of materials with properties to attenuate radiation and/or light for separating the plurality of adsorptive regions. The plurality of separating parts are formed in such a way that the surfaces of the plurality of the separating parts protrude from the surfaces of the adsorptive regions.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a biochemical analysis unit and a method for exposing a photostimulable phosphor layer using the biochemical analysis unit, and more specifically, to a substance derived from a living body and a specific substance. That can be bound to each other, and a plurality of spot-like regions containing a specific binding substance whose base sequence, base length, composition, etc. are known are formed on the carrier surface at high density,
A biochemical analysis unit obtained by selectively labeling a plurality of spot-shaped regions with a radioactive labeling substance is brought into close contact with the stimulable phosphor layer, and the stimulable phosphor layer is exposed with the radioactive labeling substance. And irradiating the stimulable phosphor layer with excitation light,
The photostimulable light emitted from the stimulable phosphor layer is photoelectrically detected to generate biochemical analysis data, and an electron beam emitted from the radiolabeled substance ( Specific for biochemical analysis unit and exposure method of stimulable phosphor layer and biogenic substance that can prevent noise caused by β-ray scattering from being generated in biochemical analysis data And a plurality of spot-shaped regions containing a specific binding substance having a known base sequence, base length, composition, etc. are formed on the carrier surface at high density,
A chemiluminescent substrate is brought into contact with a biochemical analysis unit obtained by selectively labeling a plurality of spot-like regions with a labeling substance that causes chemiluminescence by contacting the chemiluminescent substrate with a plurality of spots. Chemiluminescence is selectively emitted from a plurality of spot-shaped regions,
A unit for biochemical analysis in which chemiluminescence is selectively emitted is brought into close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed by chemiluminescence, and excitation light is emitted to the stimulable phosphor layer. The photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected to generate data for biochemical analysis, and even when analyzing a substance derived from a living body, A biochemical analysis unit and a stimulable phosphor layer capable of preventing noise caused by scattering of chemiluminescence emitted from a plurality of spot-like regions of the unit from being generated in biochemical analysis data. The present invention relates to an exposure method.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

Further, when irradiated with light, the energy of the light is absorbed, stored and recorded, and then excited by using an electromagnetic wave in a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a light detecting material, and a fixed polymer such as a protein or gene sequence is brought into contact with a chemiluminescent substance to generate chemiluminescence. A macromolecule that is selectively labeled with a labeling substance that is selectively labeled with the labeling substance,
When the chemiluminescent substance is brought into contact with the stimulable phosphor layer formed on the stimulable phosphor sheet, chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substance and the labeling substance is contained in the stimulable phosphor layer. The photostimulable phosphor is accumulated and recorded, and thereafter, the photostimulable phosphor layer is scanned with an electromagnetic wave to excite the photostimulable phosphor and photoelectrically emit the photostimulable light emitted from the photostimulable phosphor. There is known a chemiluminescence analysis system configured to reproduce data on a display means such as a CRT or a recording material such as a photographic film by performing a digital detection, generating a digital signal, performing data processing. (For example, US Pat. No. 5,028,793, British Patent Application Publication GB 2,246,197A, etc.).

These systems, which use a stimulable phosphor sheet as a radiation detecting material, do not require a chemical treatment such as a developing treatment unlike the case of using a photographic film, and also obtain the obtained digital data. By performing the data processing on (1), there is an advantage that the analysis data can be reproduced or the quantitative analysis by the computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Furthermore, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0008] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0009]

However, in the macroarray analysis system using the radiolabeled substance, when the stimulable phosphor layer is exposed by the radiolabeled substance, the surface of the carrier such as a membrane filter is exposed. Since the radiation energy of the radiolabeled substance contained in the formed spot is very large, the electron beam emitted from the radiolabeled substance is scattered in the carrier such as the membrane filter, and the radiolabeled substance contained in the adjacent spots causes The electron beam emitted from the radiolabeled substance which is incident on the region of the stimulable phosphor layer to be exposed or scattered from the radiolabeled substance is mixed with the electron beam emitted from the radiolabeled substance contained in the adjacent spots. , Incident on the region of the photostimulable phosphor layer, resulting in noise in the biochemical analysis data generated by photoelectrically detecting the photostimulable light. Generated, by quantifying the radiation amount of each spot, when analyzing the substance derived from a living organism, there is a problem that quantification is deteriorated, and formed close to the spot,
In particular, when trying to increase the density, a remarkable deterioration in the quantification was observed.

In order to prevent the noise caused by the scattering of the electron beam emitted from the radio-labeled substance contained in the adjacent spots and solve this problem, the distance between the adjacent spots must be increased. You will need to
There is a problem that the density of the spots is lowered and the inspection efficiency is lowered.

Further, in the field of biochemical analysis, cells, viruses, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, c at different positions on the surface of a carrier such as a membrane filter.
A spot-like region is formed by forming a spot-like region that can specifically bind to a substance of biological origin such as DNA, DNA, and RNA and that contains a specific binding substance whose base sequence, base length, composition, etc. are known. To the specific binding substance contained in, a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is specifically bound by hybridization or the like,
By selectively labeling and contacting the chemiluminescent substrate, chemiluminescence in the visible light wavelength range generated by contact between the chemiluminescent substrate and the labeling substance exposes the stimulable phosphor layer to expose the stimulable fluorescent layer. The energy of chemiluminescence is accumulated in the body layer, the stimulable phosphor layer is irradiated with excitation light, and the stimulable light emitted from the stimulable phosphor layer is photoelectrically detected, which is derived from a living body. It is also required to analyze the substance, but in such a case, chemiluminescence is also scattered in the carrier such as the membrane filter and mixed with chemiluminescence emitted from adjacent spot-like regions, resulting in chemiluminescence. There is a problem that noise is generated in the biochemical analysis data generated by photoelectrically detecting luminescence.

Therefore, according to the present invention, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. A unit for biochemical analysis, which was formed on the surface at high density and selectively labeled multiple spot-like regions with a radioactive labeling substance, was placed in close contact with the stimulable phosphor layer to produce a stimulable substance. Exposing the phosphor layer with a radioactive labeling substance, irradiating the stimulable phosphor layer with excitation light, and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer for biochemical analysis. Even when generating data and analyzing substances of biological origin, it is necessary to prevent noise caused by scattering of electron beams (β rays) emitted from radiolabeled substances from being generated in the biochemical analysis data. Biochemical analysis unit and photostimulable phosphor layer It is an object to provide an exposure method.

Another object of the present invention is to provide a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like. , A biochemical analysis unit obtained by selectively labeling with a labeling substance that forms chemiluminescence by contacting a chemiluminescent substrate with a plurality of spot-shaped regions formed on a carrier surface at high density, A biochemistry in which a chemiluminescent substrate is contacted to selectively emit chemiluminescence from a plurality of spot-like regions of the biochemical analysis unit, and chemiluminescence is selectively emitted from the plurality of spot-like regions. The analysis unit is brought into close contact with the stimulable phosphor layer, the stimulable phosphor layer is exposed by chemiluminescence, and the stimulable phosphor layer is irradiated with excitation light to remove the stimulable phosphor layer. Photoelectrically detecting the emitted photostimulable light To produce biochemical analysis data,
Even when analyzing substances of biological origin, it is necessary to prevent the generation of noise in the biochemical analysis data due to the scattering of chemiluminescence emitted from multiple spot-like regions of the biochemical analysis unit. It is an object of the present invention to provide a biochemical analysis unit and a method of exposing a stimulable phosphor layer that can be used.

[0014]

The object of the present invention is to:
A plurality of absorptive regions formed of an absorptive material and separated from each other, and a plurality of separating portions formed of a material having a property of attenuating radiation and / or light and separating the plurality of absorptive regions. And a plurality of separation portions are formed so that the surfaces of the plurality of separation portions respectively project from the surface of the absorptive region. .

According to the present invention, a plurality of absorptive regions formed of an absorptive material can be specifically bound to a substance derived from a living body, and the base sequence, base length, composition, etc. are known. A specific binding substance is dropped, and a substance of biological origin labeled with a radioactive labeling substance is specifically bound to the specific binding substance contained in a plurality of absorptive regions by hybridization or the like, and After selectively labeling the adsorptive region of, the biochemical analysis unit is made to face the stimulable phosphor layer formed on the stimulable phosphor sheet,
When the stimulable phosphor layer is exposed to a radioactive labeling substance contained in a plurality of absorptive regions, a plurality of separating portions that separate the plurality of absorptive regions have a property of attenuating radiation and / or light. Since the biochemical analysis unit is formed of a material having a plurality of separation parts, the separation part is placed on the stimulable phosphor layer so as to contact the surface of the stimulable phosphor layer of the stimulable phosphor sheet. By holding them together, it is possible to effectively prevent the electron beams (β rays) emitted from the radiolabeled substances contained in the plurality of adsorptive regions from being scattered in the plurality of separation parts. Therefore, it becomes possible to selectively expose the portion of the stimulable phosphor layer facing the absorptive region with an electron beam (β-ray). The stimulated emission emitted from the phosphor layer It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting it, and it is possible to generate biochemical analysis data with excellent quantitativeness. .

Further, according to the present invention, since the plurality of separation portions are formed so that the surfaces of the plurality of separation portions of the biochemical analysis unit respectively project from the surface of the absorptive region, the collimation is performed. Due to the effect, it is possible to effectively prevent the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive material in the plurality of adsorptive regions from spreading, and therefore, it is possible to face the adsorptive region. It becomes possible to selectively expose the portion of the stimulable phosphor layer that is exposed to an electron beam (β-ray), so that the stimulable light emitted from the stimulable phosphor layer is excited by excitation light. It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting, and it is possible to generate biochemical analysis data with excellent quantitativeness. Become.

Furthermore, according to the present invention, a plurality of absorptive regions formed of an absorptive material can be specifically bound to a substance of biological origin, and the base sequence, the length of the base, the composition, etc. A known specific binding substance is dropped, and a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is used to selectively label the plurality of adsorptive regions to generate a biochemical analysis unit, A chemiluminescent substrate is brought into contact with the plurality of absorptive regions of the biochemical analysis unit to cause chemiluminescence to be emitted from the plurality of absorptive regions, and chemiluminescence to be emitted from the plurality of absorptive regions. In the biochemical analysis unit, the plurality of separation parts are in contact with the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet so that the stimulable phosphor sheet has the stimulable substance. Over the fluorescent layer Together, when exposing the stimulable phosphor layer of the stimulable phosphor sheet by chemiluminescence selectively emitted from the plurality of absorptive regions of the biochemical analysis unit, a plurality of Since the plurality of separating portions for separating the absorptive region are formed of the material having the property of attenuating the radiation and / or the light, the biochemical analysis unit is provided with the plurality of separating portions for the stimulable phosphor sheet. By overlapping and holding the photostimulable phosphor layer so as to contact the surface of the stimulable phosphor layer, chemiluminescence emitted from the plurality of adsorptive regions is scattered in the plurality of separation parts. Can be effectively prevented,
Therefore, the portion of the stimulable phosphor layer facing the absorptive region can be selectively exposed by chemiluminescence, so that it is excited by the excitation light and emitted from the stimulable phosphor layer. It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting stimulated light, and to generate biochemical analysis data with excellent quantification. Will be possible.

Further, according to the present invention, since the plurality of separation portions are formed so that the surfaces of the plurality of separation portions of the biochemical analysis unit respectively project from the surface of the absorptive region, the collimation is performed. Due to the effect, it is possible to effectively prevent the chemiluminescence emitted from a plurality of adsorptive regions from spreading, and therefore, the portion of the stimulable phosphor layer facing the absorptive region is selectively selected by the chemiluminescence. Since it can be exposed to light, noise is generated in the biochemical analysis data generated by photoelectrically detecting the stimulating light emitted from the stimulable phosphor layer when excited by the excitation light. It is possible to effectively prevent this, and it is possible to generate biochemical analysis data with excellent quantification.

The above object of the present invention is also directed to a plurality of absorptive regions formed of absorptive material and spaced apart from each other, and a plurality of absorptive regions formed of a material having a property of attenuating radiation. And a plurality of separation parts for separating the surfaces of the plurality of separation parts, respectively, so as to protrude from the surface of the absorptive region, the plurality of separation parts of the biochemical analysis unit is formed. A specific binding substance having a known structure or property is dropped into a plurality of absorptive regions, and the plurality of absorptive regions are selectively labeled with a radioactive labeling substance to generate a biochemical analysis unit, In the biochemical analysis unit, the plurality of separation portions are in contact with the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet, so that the stimulable phosphor of the stimulable phosphor sheet. Layered In addition, by the radioactive labeling substance selectively contained in the plurality of absorptive regions of the biochemical analysis unit, the stimulable phosphor layer of the stimulable phosphor sheet is exposed. It is achieved by the method of exposing the stimulable phosphor layer.

According to the present invention, a plurality of absorptive regions formed of an absorptive material and formed apart from each other,
A plurality of separation parts formed of a material having a property of attenuating radiation and separating a plurality of absorptive regions, and the surfaces of the plurality of separation parts are each projected so as to protrude from the surface of the absorptive region. A specific binding substance with a known structure or property is dropped onto a plurality of absorptive regions of the unit for biochemical analysis in which the separation part of is formed, and a plurality of absorptive regions are selectively labeled with a radioactive labeling substance. To generate a biochemical analysis unit, and the biochemical analysis unit is set so that the plurality of separated portions come into contact with the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet. The stimulable phosphor layer of the stimulable phosphor sheet is superposed on the stimulable phosphor layer of the body sheet by the radioactive labeling substance selectively contained in the plurality of absorptive regions of the biochemical analysis unit. Configured to expose Therefore, at the time of exposure, it is possible to effectively prevent the electron beam (β-ray) emitted from the radiolabeled substance contained in the plurality of absorptive regions from being scattered in the plurality of separation parts. It is also possible to effectively prevent the electron beam (β-ray) emitted from the radio-labeled substance contained in the plurality of absorptive regions from spreading due to the collimation effect. Since it is possible to selectively expose the opposing photostimulable phosphor layer portions with an electron beam (β-ray), the photostimulable phosphor layers excited by the excitation light and emitted from the photostimulable phosphor layer are excited. It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting light, and it is possible to generate biochemical analysis data with excellent quantitativeness. become.

[0021] In a preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are formed on a substrate formed of a material having a property of attenuating radiation, and are separated from each other. The holes are formed by filling an adsorptive material, and the plurality of separating portions are constituted by the substrate.

According to a preferred embodiment of the present invention, the adsorptive material is filled in the plurality of holes formed in the substrate,
In the formed multiple absorptive regions, a specific binding substance that can specifically bind to a substance derived from a living body and whose base sequence, base length, composition, etc. is known is dropped and A labeled substance derived from a living body is specifically bound to a specific binding substance contained in a plurality of absorptive regions, such as by hybridization, to selectively label a plurality of absorptive regions, The biochemical analysis unit is made to face the stimulable phosphor layer formed on the stimulable phosphor sheet, and the stimulable phosphor layer is exposed to the radioactive labeling substance contained in the plurality of absorptive regions. In this case, since the substrate of the biochemical analysis unit is formed of a material having a property of attenuating the radiation, the biochemical analysis unit is arranged so that the surface of the substrate is in contact with the surface of the stimulable phosphor layer. And stimulable firefly By superimposing and holding it on the body layer, it is effective that the electron beam (β-ray) emitted from the radiolabeled substance contained in the multiple absorptive regions is scattered in the substrate of the biochemical analysis unit. Therefore, it is possible to selectively expose the portion of the stimulable phosphor layer facing the absorptive region by the electron beam (β-ray), so that the excitation light is used. Excited,
It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer, and it is excellent in quantification. It becomes possible to generate data for biochemical analysis.

According to a preferred embodiment of the present invention, a plurality of absorptive regions are formed by filling a plurality of holes formed in the substrate with an absorptive material, and the surface of the substrate is absorptive. Since a plurality of absorptive regions are formed so as to project from the surface of the absorptive region, the electron beam (β-ray) emitted from the radiolabeled substance contained in the plurality of absorptive regions is generated by the collimation effect. It can be effectively prevented from spreading, and therefore, it becomes possible to selectively expose the portion of the stimulable phosphor layer facing the absorptive region by an electron beam (β-ray). It is possible to effectively prevent the generation of noise in the biochemical analysis data generated by photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer, which is excited by light. For biochemical analysis with excellent quantification It becomes possible to generate data.

The object of the present invention is also to provide a plurality of absorptive regions formed of an absorptive material and spaced apart from each other, and a plurality of absorptive regions formed of a material having a property of attenuating light. And a plurality of separation parts for separating the surfaces of the plurality of separation parts, respectively, so as to protrude from the surface of the absorptive region, the plurality of separation parts of the biochemical analysis unit is formed. A specific binding substance having a known structure or property is dropped onto a plurality of absorptive regions, and the plurality of absorptive regions are selectively labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate. Then, a chemiluminescent substrate is brought into contact with the obtained biochemical analysis unit to cause chemiluminescence to be emitted from the plurality of absorptive regions, and chemiluminescence is emitted from the plurality of absorptive regions. In the biochemical analysis unit, the plurality of separation parts are contacted with the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet so that the stimulable fluorescent sheet of the stimulable phosphor sheet. Exposing the stimulable phosphor layer of the stimulable phosphor sheet by chemiluminescence selectively emitted from the plurality of absorptive regions of the biochemical analysis unit in superposition with the body layer. And a method of exposing a stimulable phosphor layer.

According to the present invention, a plurality of absorptive regions formed of an absorptive material and formed apart from each other,
A plurality of separating portions formed of a material having a property of attenuating light and separating a plurality of absorptive regions, each of the plurality of separating portions having a surface protruding from the surface of the absorptive region. A specific binding substance having a known structure or property is dropped onto a plurality of absorptive regions of the biochemical analysis unit in which the separation part of the is formed, and the chemiluminescence is generated by contacting the chemiluminescent substrate with a labeling substance. ,
By selectively labeling a plurality of absorptive regions, contacting the obtained biochemical analysis unit with a chemiluminescent substrate, to release chemiluminescence from the plurality of absorptive regions, and from a plurality of absorptive regions, Place the biochemical analysis unit, which emits chemiluminescence, in the stimulable phosphor sheet so that the plurality of separation parts contact the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet. The stimulable phosphor layer of the stimulable phosphor sheet is exposed by the chemiluminescence selectively emitted from the plurality of absorptive regions of the biochemical analysis unit in superposition with the stimulable phosphor layer. Because it is configured, during exposure,
It is possible to effectively prevent the chemiluminescence emitted from the plurality of adsorptive regions from being scattered in the plurality of separation portions,
Further, it is possible to effectively prevent the chemiluminescence emitted from the plurality of adsorptive regions from spreading due to the collimation effect, and therefore, the portion of the stimulable phosphor layer facing the adsorptive regions is chemiluminescent. It becomes possible to selectively expose with
It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer, and it is excellent in quantification. It becomes possible to generate data for biochemical analysis.

[0026] In a preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are formed on a substrate formed of a material having a property of attenuating light, and are separated from each other. The holes are formed by filling an adsorptive material, and the plurality of separating portions are constituted by the substrate.

According to a preferred embodiment of the present invention, the adsorptive material is filled in the plurality of holes formed in the substrate,
A specific binding substance having a known structure or property is dropped onto the formed plurality of adsorptive regions, and a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate,
By selectively labeling a plurality of absorptive regions, contacting the obtained biochemical analysis unit with a chemiluminescent substrate, to release chemiluminescence from the plurality of absorptive regions, and from a plurality of absorptive regions, Place the biochemical analysis unit, which emits chemiluminescence, in the stimulable phosphor sheet so that the plurality of separation parts contact the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet. When exposing the stimulable phosphor layer of the stimulable phosphor sheet by chemiluminescence selectively emitted from a plurality of absorptive regions of the biochemical analysis unit in superposition with the stimulable phosphor layer Since the substrate of the biochemical analysis unit is made of a material having a property of attenuating radiation, chemiluminescence emitted from a plurality of absorptive regions is effectively prevented from being scattered in a plurality of separation parts. Can and want Since it becomes possible to selectively expose the portion of the stimulable phosphor layer facing the absorptive region by chemiluminescence, the luminescence emitted from the stimulable phosphor layer is excited by excitation light. It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting the exhaustion, and to generate biochemical analysis data with excellent quantification. It will be possible.

According to a preferred embodiment of the present invention, a plurality of absorptive regions are formed by filling a plurality of holes formed in the substrate with an absorptive material, and the surface of the substrate is absorptive. Since a plurality of absorptive regions are formed so as to project from the surface of the absorptive region, it is possible to effectively prevent the chemiluminescence emitted from the plurality of absorptive regions from spreading due to the collimation effect, Therefore, the portion of the stimulable phosphor layer facing the absorptive region can be selectively exposed by chemiluminescence, so that it is excited by the excitation light and emitted from the stimulable phosphor layer. It is possible to effectively prevent noise from being generated in the biochemical analysis data generated by photoelectrically detecting stimulated light, and to generate biochemical analysis data with excellent quantification. Will be possible.

[0029] In a preferred embodiment of the present invention, a substance derived from a living body labeled with a labeling substance which causes chemiluminescence when brought into contact with a chemiluminescent substrate, has a specific binding contained in the plurality of absorptive regions. It is configured to specifically bind to a substance and selectively label the plurality of absorptive regions to generate a biochemical analysis unit.

In another preferred embodiment of the present invention, a substance derived from a living body labeled with a hapten is selectively bound to a specific binding substance contained in the plurality of adsorptive regions, and further chemiluminescence An antibody against a hapten labeled with an enzyme having a property of causing chemiluminescence by contacting with a substrate is bound to the hapten by an antigen-antibody reaction, and a plurality of adsorptive regions are selectively labeled with an enzyme. , Is configured to generate the biochemical analysis unit.

In the present invention, examples of the hapten / antibody combination include digoxigenin / anti-digoxigenin antibody, theophylline / anti-theophylline antibody, fluorescein / anti-fluorescein antibody and the like.
It is also possible to use a combination of biotin / avidin or antigen / antibody instead of the hapten / antibody.

In a preferred embodiment of the present invention, the substance derived from the living body is bound to the specific binding substance by a reaction selected from the group consisting of hybridization, antigen-antibody reaction, and receptor ligand.

[0033] In a further preferred aspect of the present invention, the plurality of absorptive regions are formed in a plurality of through-holes formed in a substrate formed of a material that attenuates radiation and / or light so as to be spaced apart from each other. Formed by being filled with an adsorptive material, and the plurality of separation portions are constituted by the substrate.

[0034] In another preferred aspect of the present invention, the plurality of absorptive regions of the biochemical analysis unit are separated from each other on a substrate formed of a material having a property of attenuating radiation and / or light. The plurality of separation portions are formed by embedding an adsorptive material in the plurality of recesses formed as described above, and the plurality of separation portions are configured by the substrate.

[0035] In a further preferred aspect of the present invention, the surfaces of the plurality of separation portions of the biochemical analysis unit each have an adsorption amount of 0.5 to 100 times the maximum width of the absorptive region. The plurality of separation portions are formed so as to project from the surface of the flexible region.

In a further preferred aspect of the present invention, the surfaces of the plurality of separation sections of the biochemical analysis unit are each 1 to 10 times the maximum width of the absorptive area, and the absorptive area is Each of the plurality of separating portions is formed so as to project from the surface of the.

In a preferred embodiment of the present invention, each of the plurality of absorptive regions has a substantially circular shape,
It is formed in the biochemical analysis unit.

[0038] In a further preferred aspect of the present invention, the surfaces of the plurality of separation portions of the biochemical analysis unit each have an adsorbability of 0.5 to 100 times the diameter of the adsorbent region. The plurality of separation portions are formed so as to project from the surface of the region.

[0039] In a further preferred aspect of the present invention, the surfaces of the plurality of separation portions of the biochemical analysis unit are each 1 to 10 times the diameter of the absorptive region.
The plurality of separating portions are formed so as to project from the surface of the absorptive region by twice.

In a preferred aspect of the present invention, each of the plurality of absorptive regions is formed in the biochemical analysis unit in a substantially rectangular shape.

In a preferred embodiment of the present invention, a plurality of absorptive regions are regularly formed in the biochemical analysis unit.

[0042] In a preferred aspect of the present invention, the biochemical analysis unit is formed with 10 or more absorptive regions.

In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 50 or more absorptive regions.

[0044] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 100 or more absorptive regions.

[0045] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 500 or more absorptive regions.

[0046] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 1000 or more absorptive regions.

In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 5000 or more absorptive regions.

[0048] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 10,000 or more absorptive regions.

[0049] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 50,000 or more absorptive regions.

[0050] In a further preferred aspect of the present invention, the biochemical analysis unit is formed with 100,000 or more absorptive regions.

In a preferred embodiment of the present invention, a plurality of absorptive regions formed in the biochemical analysis unit are
Each has a size of less than 5 square millimeters.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 0.1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 0.05 mm 2.

[0056] In a further preferred aspect of the present invention, each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 0.01 mm 2.

In the present invention, the density of the absorptive region formed in the biochemical analysis unit is determined by the type of material forming the plurality of separation parts, the type of electron beam emitted from the radiolabeled substance, and the like. .

In a preferred embodiment of the present invention, the biochemical analysis unit has a plurality of absorptive regions of 10 / adhesive regions.
It is formed with a density of square centimeters.

In a further preferred aspect of the present invention, the biochemical analysis unit has a plurality of absorptive regions.
It is formed with a density of 0 pieces / square centimeter.

In a further preferred aspect of the present invention, the biochemical analysis unit has a plurality of absorptive regions.
It is formed with a density of 00 pieces / square centimeter.

In a further preferred aspect of the present invention, the biochemical analysis unit has a plurality of absorptive regions.
It is formed with a density of 00 pieces / square centimeter.

In a further preferred aspect of the present invention, the biochemical analysis unit comprises a plurality of absorptive regions.
It is formed with a density of 000 pieces / square centimeter.

In a further preferred aspect of the present invention, the biochemical analysis unit has a plurality of absorptive regions.
It is formed with a density of 000 pieces / square centimeter.

[0064] In a further preferred aspect of the present invention, the biochemical analysis unit is provided with a plurality of absorptive regions.
It is formed with a density of 0000 pieces / square centimeter.

[0065] In a preferred aspect of the present invention, the separation section of the biochemical analysis unit transmits radiation and / or light through the separation section by a distance equal to a distance between adjacent absorptive regions. In doing so, it has the property of attenuating the energy of radiation and / or light to ⅕ or less.

[0066] In a further preferred aspect of the present invention, the separation section of the biochemical analysis unit is arranged so that radiation and / or light penetrates through the separation section by a distance equal to a distance between adjacent absorptive regions. When transmitted, it has a property of attenuating the energy of radiation and / or light to 1/10 or less.

[0067] In a further preferred aspect of the present invention, the separation section of the biochemical analysis unit is arranged such that radiation and / or light penetrates through the separation section by a distance equal to a distance between adjacent absorptive regions. When transmitted, it has a property of attenuating the energy of radiation and / or light to 1/50 or less.

[0068] In a further preferred aspect of the present invention, the separation section of the biochemical analysis unit is arranged so that radiation and / or light pass through the separation section by a distance equal to a distance between adjacent absorptive regions. When transmitted, it has a property of attenuating the energy of radiation and / or light to 1/100 or less.

[0069] In a further preferred aspect of the present invention, the separation section of the biochemical analysis unit is arranged so that radiation and / or light penetrates through the separation section by a distance equal to a distance between adjacent absorptive regions. When transmitted, it has the property of attenuating the energy of radiation and / or light to 1/500 or less.

[0070] In a further preferred aspect of the present invention, the separation section of the biochemical analysis unit is arranged so that radiation and / or light are distributed in the separation section by a distance equal to a distance between adjacent absorptive regions. When transmitted, it has a property of attenuating the energy of radiation and / or light to 1/1000 or less.

In the present invention, the material used for forming the separation portion of the biochemical analysis unit is not particularly limited as long as it has a property of attenuating radiation and / or light. Either an inorganic compound material or an organic compound material can be used, but a metal material,
Ceramic or plastic materials are particularly preferred.

In the present invention, inorganic compound materials that can be preferably used to form the separation part of the biochemical analysis unit and can attenuate radiation and / or light include, for example, gold, silver, copper, Metals such as zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, silicon nitride, etc. Silicon materials; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material that can be used to form the separation part of the biochemical analysis unit and can attenuate radiation and / or light. Polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethylmethacrylate and butyl acrylate / methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoroethylene Polycarbonate; Polyester such as polyethylene naphthalate or polyethylene terephthalate; Nylon such as nylon 6, nylon 6,6, nylon 4,10; polyimide; polysulfone; polyphenyl Nsarufaido; phenolic resins such as novolak; silicon resins such as polydiphenylsiloxane epoxy resin; polyurethane; polystyrene;
Butadiene-styrene copolymer; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, and hydroxypropylmethylcellulose; chitin; chitosan; sumac; polyamide such as gelatin, collagen and keratin, and copolymerization of these polymer compounds Examples include coalescing. These may be composite materials, and may be filled with metal oxide particles, glass fibers, or the like, if desired, or may be used by blending with an organic compound material.

Generally, the larger the specific gravity is, the higher the radiation attenuating ability is. Therefore, the separation section of the biochemical analysis unit is
It is preferably formed of a compound material or composite material having a specific gravity of 1.0 g / cm ³ or more, and a specific gravity of 1.5 g / cm ^3.
It is particularly preferable that it is formed of a compound material or a composite material having a cm ³ or more and 23 g / cm ³ or less.

In general, the greater the scattering and / or absorption of light, the higher the light attenuating ability. Therefore, the separation section of the biochemical analysis unit has an absorbance of 0.3 or more per cm of thickness. It is preferable that the absorbance is 1 or more per cm of thickness, and it is further preferable. Here, for the absorbance, an integrating sphere is placed immediately after the plate body having a thickness of Tcm,
The transmitted light amount A at the wavelength of the probe light or the emission light used for measurement is measured by a spectrophotometer, and A /
It is obtained by calculating T. In order to improve the light attenuating ability, a light scatterer or a light absorber may be contained in the separation part of the biochemical analysis unit. As the light scatterer, fine particles of a material different from the material forming the separation part of the biochemical analysis unit are used, and as the light absorber, a pigment or a dye is used.

In a further preferred aspect of the present invention, the substrate of the biochemical analysis unit is formed of a flexible material.

According to a further preferred aspect of the present invention, since the substrate of the biochemical analysis unit is formed of a flexible material, the biochemical analysis unit is curved to form a hybridization reaction solution. It is possible to specifically bind a substance of biological origin to a specific binding substance by contacting a reaction solution such as It becomes possible to specifically bind a substance of biological origin to the binding substance.

In the present invention, a porous material or a fiber material is preferably used as the adsorptive material for forming the adsorptive region. The porous material and the fiber material may be used together to form the adsorptive region.

In the present invention, the porous material used for forming the adsorptive region may be either an organic material or an inorganic material, or an organic / inorganic composite.

In the present invention, the organic porous material used to form the adsorptive region is not particularly limited, but a carbon porous material such as activated carbon or a porous material capable of forming a membrane filter. Are preferably used. Specifically, nylon 6, nylon 6,
6, nylons such as nylon 4, 10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid such as alginic acid, calcium alginate, alginic acid / polylysine polyion complex; polyolefins such as polyethylene and polypropylene; Examples thereof include polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluoride such as polytetrafluoride, and copolymers or composites thereof.

In the present invention, the inorganic porous material used for forming the absorptive region is not particularly limited, but preferably, for example, platinum, gold, iron,
Examples thereof include metals such as silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fiber material used for forming the adsorptive region is not particularly limited, but preferably, for example, nylon 6, nylon 6,6, nylon 4,10, etc. Examples include nylons, cellulose derivatives such as nitrocellulose, cellulose acetate, and cellulose acetate butyrate.

In the present invention, the stimulable phosphor used for storing the energy of the radiation can store the energy of the radiation and is excited by an electromagnetic wave.
It is not particularly limited as long as it can release the energy of accumulated radiation in the form of light,
Those that can be excited by light in the visible light wavelength range are preferable. Specifically, for example, US Pat. No. 4,239,9
No. 68 alkaline earth metal fluorohalide-based phosphor (Ba1-xM ²⁺ x) FX: yA (here,
M ²⁺ is at least one alkaline earth metal element selected from the group consisting of Mg, Ca, Sr, Zn and Cd,
X is at least one halogen selected from the group consisting of Cl, Br and I, A is Eu, Tb, Ce, Tm, D
at least one trivalent metal element selected from the group consisting of y, Pr, Ho, Nd, Yb and Er, and x is 0 ≦ x ≦
0.6 and y are 0 ≦ y ≦ 0.2. ), Japanese Patent Laid-Open No. 2-2
Alkaline earth metal fluoride halide phosphor SrFX: Z (where X is Cl,
At least one halogen selected from the group consisting of Br and I, Z is Eu or Ce. ), JP-A-59
-56479 disclosed europium-activated composite halogen-based phosphor BaFX.xNaX ': aEu ²⁺
(Here, each of X and X ′ is at least one halogen selected from the group consisting of Cl, Br and I, x is 0 <x ≦ 2, and a is 0 <a ≦ 0.2. ), M which is a cerium-activated trivalent metal oxyhalogen-based phosphor disclosed in JP-A-58-69281.
OX: xCe (where M is Pr, Nd, Pm, Sm,
Eu, Tb, Dy, Ho, Er, Tm, Yb and Bi
At least one trivalent metal element selected from the group consisting of, X is one or both of Br and I, x
Is 0 <x <0.1. ), U.S. Pat. No. 4,53
Cerium-activated rare earth oxyhalogen-based phosphor disclosed in No. 9,137, LnOX: xCe (here, L
n is at least one rare earth element selected from the group consisting of Y, La, Gd and Lu, and X is Cl, Br and I
At least one halogen selected from the group consisting of, x
Is 0 <x ≦ 0.1. ) And US Pat. No. 4,9
Europium activated complex halide systems are disclosed in EP 62,047 phosphor ^{M II FX · aM I X '} · bM' II X '' 2
・ CM ^III X ^''' 3 ・ xA: yEu ²⁺ (here, M ^II
Is at least one alkaline earth metal element selected from the group consisting of Ba, Sr and Ca, and M ^I is Li, N
at least one alkali metal element selected from the group consisting of a, K, Rb and Cs, M ′ ^II is Be and Mg
At least one divalent metal element selected from the group consisting of, M ^III is at least one trivalent metal element selected from the group consisting of Al, Ga, In and Tl, A is at least one metal oxide, and X is Cl. , Br and I, at least one halogen selected from the group consisting of, X ', X
^{″ And} X ^{′ ″} are at least one halogen selected from the group consisting of F, Cl, Br and I, and a is 0
≦ a ≦ 2, b is 0 ≦ b ≦ 10 ⁻² , c is 0 ≦ c ≦ 1
0 ⁻² and a + b + c ≧ 10 ⁻² , and x is
When 0 <x ≦ 0.5, y is 0 <y ≦ 0.2. )
Can be preferably used.

In the present invention, the stimulable phosphor used for storing chemiluminescent energy is capable of storing light energy in the visible light wavelength region, and is excited by electromagnetic waves to absorb the accumulated light. The energy is not particularly limited as long as it can emit energy in the form of light, but those capable of being excited by light in the visible light wavelength range are preferable. Specifically, for example, a metal halophosphate-based phosphor, a rare earth element-activated sulfide-based phosphor, an aluminate-based phosphor, a silicate-based phosphor, a fluoride-based phosphor, and two or more thereof. Those selected from the group consisting of the mixture of are preferably used. Of these, rare earth element-activated sulfide-based phosphors are preferable, and US Pat. No. 5,029,253 and 4,983,834 are particularly preferable.
Rare earth element-activated alkaline earth metal sulfide-based phosphor disclosed in Japanese Patent Publication No. 2001-1980
Zn ₂ GeO ₄ : Mn, disclosed in Japanese Patent No. 31545,
Zinc germanate phosphors such as V and Zn ₂ GeO ₄ : Mn, alkaline earth aluminate phosphors such as Sr ₄ Al ₁₄ O ₂₅ : Ln (Ln is a rare earth) disclosed in JP 2001-123162 A, Y _0.8 Lu _1.2 Si
O ₅ : Ce, Zr, GdOCl: Ce disclosed in JP-B-6-31904 and the like are preferably used.

[0085]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

As shown in FIG. 1, in the present embodiment, the biochemical analysis unit 1 has a property of attenuating radiation and is formed of a flexible metal such as stainless steel and has a large number of elements. The substrate 2 in which the substantially circular through holes 3 are formed at a high density is provided, and a large number of through holes 3 are provided inside.
A large number of absorptive regions 4 are formed in a dot shape by being filled with an absorptive material such as nylon 6.

Although not shown exactly in FIG. 1, in the present embodiment, the substantially circular adsorptive regions 4 having a size of about 0.01 mm 2 are arranged in a matrix of 120 columns × 160 rows. Through holes 3 are formed in the substrate 2 so as to be formed regularly, so that a total of 1
The absorptive region 4 of 9200 is formed.

FIG. 2 is a schematic sectional view of the biochemical analysis unit 1.

As shown in FIG. 2, a large number of absorptive regions 4 are filled with nylon 6 in the through holes 3 formed in the substrate 2 so that the surface thereof is lower than the surface of the substrate 2. In this embodiment, the difference in height between the surface of each absorptive region 4 and the surface of the substrate 2, that is, the distance between the surface of each absorptive region 4 and the surface of the substrate 2 is ,
The adsorptive material is filled in the through holes 3 so as to have a diameter five times the diameter of the through holes 3 to form the adsorptive regions 4.

FIG. 3 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 3, for example, a solution containing a plurality of different cDNAs having known base sequences as the specific binding substances was prepared using the spotting device 5. The chemical analysis unit 1 is dropped in a large number of absorptive regions 4 and fixed in the absorptive regions 4.

As shown in FIG. 3, the spotting device 5 comprises an injector 6 for injecting a solution of a specific binding substance toward the biochemical analysis unit 1 and a CCD camera 7, and a CCD camera 7 The tip of the injector 6 and a solution of the specific binding substance, for example, cD
While observing the absorptive region 4 of the biochemical analysis unit 1 on which the NA solution should be dropped, the tip of the injector 6 and
When the center of the absorptive region 4 on which the solution of the specific binding substance should be dropped coincides, the specific binding substance is dropped from the injector 6, and a large number of biochemical analysis units 1 are configured. It is ensured that the solution of the specific binding substance can be accurately dropped into the adsorptive region 4.

FIG. 4 is a schematic cross sectional view of a hybridization reaction container.

As shown in FIG. 4, the hybridization reaction container 8 has a cylindrical shape, and a hybridization reaction solution 9 containing a substance derived from a living body labeled with a labeling substance is housed inside.

In this embodiment, a hybridization reaction solution 9 containing a substance of biological origin labeled with a radioactive labeling substance is prepared and housed in the hybridization reaction container 8.

During hybridization, the biochemical analysis unit 1 in which a specific binding substance such as cDNA is dropped onto the absorptive region 4 in a large number of through holes 3 is inserted into the hybridization reaction container 8. Board 2
Is formed of a metal such as stainless steel, the biochemical analysis unit 1 is curved as shown in FIG. Can be inserted inside.

As shown in FIG. 4, the hybridization reaction container 8 is driven by a driving means (not shown).
The hybridization reaction container 8 is configured so as to be rotatable around an axis, and the biochemical analysis unit 1 is curved so as to follow the inner wall of the hybridization reaction container 8.
Since the hybridization reaction container 8 is inserted into the inside of the adsorption reaction chamber 8 by rotating the hybridization reaction container 8, even if the hybridization reaction solution 9 is small
The specific binding substance such as cDNA dropped inside is constructed so that the substance of biological origin which is labeled with a radioactive labeling substance and is contained in the hybridization reaction solution 9 can be selectively hybridized. ing.

FIG. 5 shows a method for exposing the stimulable phosphor layer formed on the stimulable phosphor sheet with the radioactive labeling substance contained in the many absorptive regions 4 of the biochemical analysis unit 1. It is a schematic sectional drawing.

As shown in FIG. 5, upon exposure, the stimulable phosphor layer 12 formed uniformly on one surface of the support 11 of the stimulable phosphor sheet 10 was used as a biochemical analysis unit. The stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 1 so as to come into contact with the surface of the first substrate 2.

At this time, an electron beam (β-ray) is emitted from the radio-labeled substance contained in the many absorptive regions 4, but the stainless steel having the property that the substrate 2 of the biochemical analysis unit 1 attenuates the radiation. Since it is formed of a metal such as, the electron beam (β-ray) emitted from the radiolabel substance contained in the adsorptive region 4 is reliably prevented from being scattered in the substrate 2, and the adsorption The height difference between the surface of the absorptive region 4 and the surface of the substrate 2, that is, the distance between the surface of the absorptive region 4 and the surface of the substrate 2 is 5 times the diameter of the through hole 3. The material is filled in a large number of through holes 3,
Since the absorptive region 4 is formed, the collimation effect prevents the electron beam (β-ray) emitted from the radiolabeled substance contained in each absorptive region 4 from spreading, and therefore biochemical analysis is performed. Of the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 facing each absorptive region 4 of the unit 1 for use in the electron beam emitted from the radiolabel substance contained in each absorptive region 4 ( Only β rays can be used to selectively expose.

Thus, radiation data is recorded on the stimulable phosphor layer 12 formed on the stimulable phosphor sheet 10.

FIG. 6 shows an example of a scanner that reads the radiation data of the radiolabeled substance recorded in the stimulable phosphor layer 12 formed on the stimulable phosphor sheet 10 to generate biochemical analysis data. FIG. 7 is a schematic perspective view showing the same, and FIG. 7 is a schematic perspective view showing details of the vicinity of the photomultiplier.

The scanner shown in FIG. 6 has a stimulable phosphor layer 1 formed on a support 11 of a stimulable phosphor sheet 10.
The radiological data of the radiolabeled substance recorded in 2 and the fluorescence data of the fluorescent substance such as the electrophoretic data of the denatured DNA labeled with the fluorescent dye recorded on the gel support or the transfer support are readable. And a first laser excitation light source 21 that emits a laser light 24 having a wavelength of 640 nm and a laser light 24 having a wavelength of 532 nm.
And a third laser excitation light source 23 which emits a laser beam 24 having a wavelength of 473 nm.

In this embodiment, the first laser excitation light source 21 is composed of a semiconductor laser light source,
Laser excitation light source 22 and third laser excitation light source 23
Is composed of a second harmonic generation element.

The laser light 24 generated by the first laser excitation light source 21 is collimated by the collimator lens 25 and then reflected by the mirror 26. First
In the optical path of the laser light 24 emitted from the laser excitation light source 21 and reflected by the mirror 26, the first dichroic mirror 27 that transmits the laser light 4 of 640 nm and reflects the light of 532 nm wavelength and the 532 nm or longer A second dichroic mirror 28 that transmits light of a wavelength and reflects light of a wavelength of 473 nm is provided.
Laser light 24 generated by the laser excitation light source 21 of
Passes through the first dichroic mirror 27 and the second dichroic mirror 28 and enters the mirror 29.

On the other hand, the laser light 24 generated from the second laser excitation light source 22 is passed by the collimator lens 30.
After being made into parallel light, it is reflected by the first dichroic mirror 27, its direction is changed by 90 degrees, and
The light passes through the dichroic mirror 28 and enters the mirror 29.

The laser light 24 generated from the third laser excitation light source 23 is collimated by the collimator lens 31, and then the second dichroic mirror 2 is used.
After being reflected by 8 and changing its direction by 90 degrees,
It is incident on the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
A perforated mirror 34 formed by a concave mirror having a hole 33 formed in the center is arranged in the optical path of No. 4, and the laser light 24 reflected by the mirror 32 passes through the hole 33 of the perforated mirror 34. It passes through and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

The optical head 35 is provided with a mirror 36 and an aspherical lens 37. The laser light 24 incident on the optical head 35 is reflected by the mirror 36, and the aspherical lens 37 causes the glass plate of the stage 40 to be reflected. It is incident on the stimulable phosphor sheet 10 placed on 41, a gel support or a transfer support.

When the laser beam 24 is incident on the stimulable phosphor layer 12 of the stimulable phosphor sheet 10, it is contained in the stimulable phosphor layer 12 formed on the support 11 of the stimulable phosphor sheet 10. Exciting stimulable phosphor is excited to emit stimulable light 45, and laser light 24 is emitted to the gel support or the transfer support.
When is incident, the fluorescent substance contained in the gel support or the transfer support is excited, and fluorescence 45 is emitted.

The photostimulable light 45 emitted from the stimulable phosphor sheet 10 or the fluorescence 45 emitted from the gel support or the transfer support is collected on the mirror 36 by the aspherical lens 37 provided on the optical head 35. The light is reflected, is reflected by the mirror 36 to the same side as the optical path of the laser light 24, is made into parallel light, and is incident on the concave mirror 38.

The photostimulable light 45 or the fluorescence 45 incident on the concave mirror 38 is reflected by the concave mirror 38,
The light enters the perforated mirror 34.

The photostimulable light 45 or the fluorescence 45 incident on the perforated mirror 34 is reflected downward by the perforated mirror 34 formed by a concave mirror to enter the filter unit 48, as shown in FIG. Then, light of a predetermined wavelength is cut off, enters the photomultiplier 50, and is detected photoelectrically.

As shown in FIG. 7, the filter unit 48 includes four filter members 51a, 51b, 51c,
51d, the filter unit 48 is configured to be movable in the left-right direction in FIG. 7 by a motor (not shown).

FIG. 8 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 8, the filter member 51
a includes a filter 52 a, and the filter 52 a uses the first laser excitation light source 21 to excite a fluorescent substance such as a fluorescent dye contained in the gel support or the transfer support to read fluorescence 45. It is a filter member sometimes used, which cuts light with a wavelength of 640 nm.
It has a property of transmitting light having a wavelength longer than nm.

FIG. 9 is a sectional view taken along the line BB of FIG.

As shown in FIG. 9, the filter member 51
b includes a filter 52b, and the filter 52b uses the second laser excitation light source 22 to excite a fluorescent substance such as a fluorescent dye contained in the gel support or the transfer support to read the fluorescence 45. It is a filter member that is used at times and cuts light with a wavelength of 532 nm.
It has a property of transmitting light having a wavelength longer than nm.

FIG. 10 is a sectional view taken along the line CC of FIG.

As shown in FIG. 10, the filter member 5
1c includes a filter 52c, and the filter 52c includes a third filter 52c.
Is a filter member used when the fluorescent substance such as a fluorescent dye contained in the gel support or the transfer support is excited by using the laser excitation light source 23 of FIG. Cut the light of 47
It has a property of transmitting light having a wavelength longer than 3 nm.

FIG. 11 is a sectional view taken along the line DD of FIG.

As shown in FIG. 11, the filter member 5
1d includes a filter 52d, and the filter 52d includes a first
The stimulable phosphor sheet 1 using the laser excitation light source 21 of
0 is a filter used when exciting the stimulable phosphor layer 12 formed on the support 11 of 0 to read the stimulable light 45 emitted from the stimulable phosphor layer 12, It has a property of transmitting only light in the wavelength region of stimulated emission emitted from the body layer 12 and cutting light having a wavelength of 640 nm.

Therefore, depending on the laser excitation light source to be used, the filter members 51a, 51b, 51c, 51d.
Is selectively located in front of the photomultiplier 50, the photomultiplier 50 can photoelectrically detect only the light to be detected.

The analog data photoelectrically detected by the photomultiplier 50 and generated are converted into digital data by the A / D converter 53 and sent to the data processor 54.

Although not shown in FIG. 6, the optical head 35 is configured to be movable in the X and Y directions in FIG. 6 by the scanning mechanism, and the stimulable phosphor sheet 1 is formed.
The entire surface of the stimulable phosphor layer 12 formed on the support 11 of No. 0 or the entire surface of the gel support or the transfer support is configured to be scanned by the laser light 24.

FIG. 12 is a schematic plan view of the scanning mechanism of the optical head. In FIG. 12, for simplification, the optical system except the optical head 15 and the optical paths of the laser light 24 and the stimulated light 45 or the fluorescence 45 are omitted.

As shown in FIG. 12, the optical head 35
The scanning mechanism that scans the substrate includes a substrate 60, and a sub-scanning pulse motor 61 and a pair of rails 62, 62 are provided on the substrate 60.
And 6 are fixed on the substrate 60 and are movable on the substrate 60 in the sub-scanning direction indicated by the arrow Y in FIG.
3 and 3 are provided.

A threaded hole (not shown) is formed in the movable substrate 63, and a threaded rod 64 rotated by the sub-scanning pulse motor 61 is formed in this hole. Engaged.

A main scanning pulse motor 65 is provided on the movable substrate 63. The main scanning pulse motor 65 connects the endless belt 66 to the adsorbing regions 4 adjacent to each other formed in the biochemical analysis unit 1. It is configured such that it can be driven intermittently at a pitch equal to the distance. Optical head 3
5 is fixed to the endless belt 66, and is configured to be moved in the main scanning direction indicated by an arrow X in FIG. 12 when the endless belt 66 is driven by the main scanning pulse motor 65. There is. In FIG. 12, 67 is a linear encoder that detects the position of the optical head 35 in the main scanning direction, and 68 is a slit of the linear encoder 67.

Therefore, the endless belt 66 is driven in the main scanning direction by the main scanning pulse motor 65, and the movable substrate 63 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 61. 12, the optical head 35 is moved in the X direction and the Y direction in FIG. 12, and the entire surface of the stimulable phosphor layer 12 formed on the stimulable phosphor sheet 10 by the laser beam 24 or the biochemical analysis unit 1 is used. All the absorptive regions 4 formed in the are scanned.

FIG. 13 is a block diagram showing the control system, input system and drive system of the scanner shown in FIG.

As shown in FIG. 13, the control system of the scanner is a control unit 7 for controlling the entire scanner.
0, and the input system of the scanner is equipped with a keyboard 71 which is operated by the user and can input various instruction signals.

As shown in FIG. 13, the drive system of the scanner includes a main scanning pulse motor 65 for moving the optical head 35 in the main scanning direction, and a sub scanning pulse motor 61 for moving the optical head 35 in the sub scanning direction. A filter unit motor 72 for moving the filter unit 48 including the four filter members 51a, 51b, 51c, 51d is provided.

The control unit 70 includes a first laser pumping light source 21, a second laser pumping light source 22 or a third laser pumping light source 22.
In addition to selectively outputting a drive signal to the laser excitation light source 23, the drive signal can be output to the filter unit motor 72.

The scanner configured as described above uses the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 as described below. The layer 12 is exposed and the radiation data of the radiolabeled substance recorded on the stimulable phosphor sheet 10 is read to generate biochemical analysis data.

First, the user mounts the stimulable phosphor sheet 10 on the glass plate 41 of the stage 40 so that the stimulable phosphor layer 12 contacts the surface of the glass plate 41.

Next, the user operates the keyboard 7
An instruction signal to the effect that the radiation data recorded in the stimulable phosphor layer 12 formed on the support 11 of the stimulable phosphor sheet 10 should be read is input to 1.

The instruction signal input to the keyboard 71 is
Input to the control unit 70, the control unit 70 outputs a drive signal to the filter unit motor 72 according to the instruction signal, and the filter unit 4
8 to move the filter member 51d having a filter 52d having a property of transmitting only light in the wavelength range of stimulable light emitted from the stimulable phosphor and cutting light having a wavelength of 640 nm. It is located in the optical path of the light 45.

Then, the control unit 70 outputs a drive signal to the first laser excitation light source 21, activates the first laser excitation light source 21, and emits the laser light 24 having a wavelength of 640 nm.

The laser light 24 emitted from the first laser excitation light source 21 is made into parallel light by the collimator lens 25, then enters the mirror 26 and is reflected.

Laser light 2 reflected by the mirror 26
4 passes through the first dichroic mirror 27 and the second dichroic mirror 28, and enters the mirror 29.

The laser light 24 that has entered the mirror 29 is reflected by the mirror 29, and then enters the mirror 32 and is reflected.

Laser light 2 reflected by the mirror 32
4 passes through the hole 33 of the perforated mirror 34 and enters the concave mirror 38.

The laser light 24 incident on the concave mirror 38
Is reflected by the concave mirror 38, and the optical head 3
It is incident on 5.

Laser light 24 incident on the optical head 35
Is reflected by the mirror 36 and is condensed by the aspherical lens 37 on the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 placed on the stage 40 glass plate 41.

As a result, the stimulable phosphor contained in the stimulable phosphor layer 12 formed on the stimulable phosphor sheet 10 was
Excited light 45 is emitted from the stimulable phosphor that is excited by the laser light 24 and exposed by the radioactive labeling substance.

The photostimulable light 45 emitted from the photostimulable phosphor contained in the photostimulable phosphor layer 12 is collected by the aspherical lens 37 provided in the optical head 35, and is reflected by the mirror 36.
Is reflected to the same side as the optical path of the laser light 24, becomes parallel light, and is incident on the concave mirror 38.

The photostimulable light 45 incident on the concave mirror 38 is
The perforated mirror 3 is reflected by the concave mirror 38.
It is incident on 4.

Photostimulation 45 incident on the perforated mirror 34
Is reflected downward by the perforated mirror 34 formed by the concave mirror, and enters the filter 52d of the filter unit 48, as shown in FIG.

The filter 52d has a property of transmitting only the light in the wavelength region of the stimulable light emitted from the stimulable phosphor and cutting off the light of the wavelength of 640 nm, and therefore, the excitation light of 640 nm is used. The light having the wavelength of is cut off, and only the light in the wavelength range of the photostimulated light is transmitted through the filter 52d and is photoelectrically detected by the photomultiplier 50.

As described above, the optical head 35 includes the substrate 6
12, the main scanning pulse motor 65 moves the substrate 62 in the X direction in FIG. 12, and the sub scanning pulse motor 61 moves the substrate 62 in the Y direction in FIG. Therefore, the stimulable phosphor layer 1 formed on the support 11 of the stimulable phosphor sheet 10
The entire surface of 2 is scanned by the laser beam 24, and the photomultiplier 50 photoelectrically detects the photostimulable light 45 emitted from the photostimulable phosphor contained in the photostimulable phosphor layer 12, The radiation data of the radiolabeled substance recorded in the stimulable phosphor layer 12 can be read to generate analog data for biochemical analysis.

The analog data photoelectrically detected by the photomultiplier 50 and generated is converted into digital data by the A / D converter 53 and sent to the data processor 54.

On the other hand, by reading the fluorescence data of the fluorescent substance such as the electrophoresis data of the denatured DNA labeled with the fluorescent dye recorded on the gel support or the transfer support,
When generating the data for biochemical analysis, first, the user sets the gel support or the transfer support on which the fluorescence data is recorded on the glass plate 41 of the stage 40.

[0157] Next, the user operates the keyboard 7
When the type of the fluorescent substance is input to 1, the input fluorescent substance from the first laser excitation light source 21, the second laser excitation light source 22 and the third laser excitation light source 23 by the control unit 70. A laser excitation light source that emits a laser beam 24 having a wavelength that can efficiently excite light is selected, and the three filter members 51a, 51
The property of cutting the light of the wavelength of the laser light 24 used to excite the input fluorescent substance from b and 51c and transmitting the light having a wavelength longer than the wavelength of the laser light 24 used to excite the fluorescent substance. A filter member having is selected.

Next, the entire surface of the gel support or the transfer support is scanned by the laser beam 24, and the fluorescence 45 emitted from the fluorescent substance is photoelectrically detected by the photomultiplier 50 to generate analog data. Then, the data is digitized by the A / D converter and biochemical analysis data is generated.

In this embodiment, the biochemical analysis unit 1 is made of a flexible metal such as stainless steel having a property of attenuating radiation, and a large number of through holes 3 are formed at high density. The plurality of through-holes 3 have a height difference between the surface and the surface of the substrate 2, that is, the distance between the surface of the absorptive region 4 and the surface of the substrate 2 penetrates through the through-holes 3. Nylon 6 to be 5 times the diameter of hole 3
A large number of absorptive regions 4 are formed by filling absorptive material such as.

In the biochemical analysis, a plurality of c's are placed in the absorptive region 4 in the many through holes 3 of the biochemical analysis data 2.
Specific binding substances such as DNA are spotting devices 5
Furthermore, a substance derived from a living body labeled with a radiolabeled substance is selectively hybridized with a specific binding substance such as cDNA contained in a large number of adsorptive regions 4 of the biochemical analysis data 1 by the above method. After being soyed, the stimulable phosphor layer 12 formed on the surface of the support 11 of the stimulable phosphor sheet 10 is contacted with the surface of the substrate 2 of the biochemical analysis unit 1 so that the stimulable phosphor layer 12 is in contact. The body sheet 10 is superposed on the biochemical analysis unit 1 and the stimulable phosphor 12 of the stimulable phosphor sheet 10 is transferred by the radiolabeling substance.
Is exposed.

Therefore, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is made of a metal such as stainless steel having a property of attenuating radiation, a large number of through holes are formed during exposure. Electron beam (β-ray) emitted from the radiolabeled substance contained in the adsorptive region 4 in 3
However, it can be effectively prevented from being scattered in the substrate 2 of the biochemical analysis unit 1.

Further, according to this embodiment, the absorptive region 4
There are many adsorbent materials so that the difference in height between the surface of the substrate 2 and the surface of the substrate 2, that is, the distance between the surface of the adsorptive region 4 and the surface of the substrate 2 is 5 times the diameter of the through hole 3. Since a large number of absorptive regions 4 are formed by being filled in the through-holes 3 of the, the electrons emitted from the radiolabeled substance contained in the absorptive regions 4 in a large number of through-holes 3 due to the collimation effect. It is possible to effectively prevent the line (β line) from spreading.

Therefore, according to this embodiment, the portion of the stimulable phosphor layer 12 facing the through hole 3 is irradiated with an electron beam (β
Line) allows selective exposure,
The photostimulable phosphor layer 12 is excited by the laser light 24.
Photostimulated photoluminescence emitted from the device can be detected photoelectrically to prevent noise from being generated in the generated biochemical analysis data, generating highly quantitative biochemical analysis data. It becomes possible to do.

FIG. 14 is a schematic sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 14, the biochemical analysis unit 1 according to the present embodiment has a property of attenuating radiation and is formed of a flexible metal such as stainless steel. The substrate 2 in which the circular concave portions 15 are formed with high density is provided, and the large number of concave portions 15 are filled with an absorptive material such as nylon 6 to form an absorptive region 4
Are formed in a dot shape.

Although not shown in FIG. 14, in the present embodiment, the substantially circular absorptive regions 4 having a size of about 0.07 mm 2 are regularly arranged in a matrix of 120 columns × 160 rows. To be formed on the substrate 2
At this time, the recess 15 is formed, so that a total of 19
200 absorptive regions 4 are formed.

As shown in FIG. 14, in this embodiment, inside the large number of recesses 15 formed in the substrate 2, the difference in height between the surface and the surface of the substrate 2, that is,
The distance between the surface of the absorptive region 4 and the surface of the substrate 2 is equal to the recess 1
The adsorbent material is filled so that the diameter becomes 3 times the diameter of 5,
A large number of absorptive regions 4 are formed.

Also in this embodiment, as in the biochemical analysis unit 1 shown in FIGS. 1 and 2, a large number of recesses 1 are formed by the spotting device 5 shown in FIG.
After a specific binding substance such as cDNA is dropped into the absorptive region 4 formed in 5 as shown in FIG.
The biochemical analysis unit 1 is curved in a hybridization reaction container 8 containing a hybridization reaction solution 9 containing a substance derived from a living body labeled with a radioactive labeling substance, and the hybridization reaction container 8
The specific binding substance such as cDNA, which is inserted along the inner wall of the, and is dropped in the adsorptive region 4 formed in the large number of recesses 15, is labeled with a radioactive labeling substance and is contained in the hybridization reaction solution 9. The biologically derived substance thus selectively hybridized.

Next, as shown in FIG. 5, the stimulable phosphor layer 12 formed uniformly on one surface of the support 11 of the stimulable phosphor sheet 10 is used as the biochemical analysis unit 1. The stimulable phosphor sheet 10 is superposed on the biochemical analysis unit 1 so as to come into contact with the surface of the substrate 2, and the stimulability is increased by the radioactive labeling substance contained in the absorptive region 4 in the large number of recesses 15. The stimulable phosphor layer 12 formed on the support 11 of the phosphor sheet 10 is exposed.

Therefore, according to this embodiment, since the substrate 2 of the biochemical analysis unit 1 is formed of a metal such as stainless steel having a property of attenuating radiation, a large number of recesses 15 are formed during exposure. Beam (β-ray) emitted from the radiolabeled substance contained in the adsorptive region 4 inside
However, it can be effectively prevented from being scattered in the substrate 2 of the biochemical analysis unit 1.

Further, according to this embodiment, the absorptive region 4
The height difference between the surface of the adsorbent material and the surface of the substrate 2, that is, the distance between the surface of the adsorbent region 4 and the surface of the substrate 2 is three times the diameter of the concave portion 15. Since a large number of absorptive regions 4 are formed by filling the recesses 15, an electron beam (β generated from the radio-labeled substance contained in the absorptive regions 4 in a large number of the recesses 15 due to the collimation effect is formed. It is possible to effectively prevent the line) from spreading.

Therefore, according to the present embodiment, the concave portion 1
Since the portion of the stimulable phosphor layer 12 facing the opening of 5 and the absorptive region 4 can be selectively exposed by an electron beam (β-ray), it is excited by the laser beam 24, The photostimulable light emitted from the photostimulable phosphor layer 12 is photoelectrically detected to prevent noise from being generated in the generated biochemical analysis data, which is excellent in quantification. It becomes possible to generate data for biochemical analysis.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above-mentioned embodiment, a plurality of cDNAs having different known base sequences are used as the specific binding substance, but the specific binding substance usable in the present invention is not limited to the cDNA. Hormones, tumor markers, enzymes, antibodies, antigens,
Abzyme, other proteins, nucleic acids, cDNA, D
All specific binding substances, such as NA and RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. are known, should be used as the specific binding substance of the present invention. You can

Further, in the above-mentioned embodiment, the substance of biological origin labeled with the radiolabel substance is hybridized with the specific binding substance. However, the substance of biological origin is hybridized with the specific binding substance. It is not always necessary that the substance derived from the living body be specifically bound to the specific binding substance by a reaction such as an antigen-antibody reaction or a receptor ligand, instead of hybridization.

Further, in the above-mentioned embodiment, the substrate 2 of the biochemical analysis unit 1 has flexibility, but it is not always necessary to have flexibility.

In the above embodiment, 1920
A substantially circular adsorptive region 4 having a size of 0 and a size of about 0.01 mm 2 is regularly formed in the biochemical analysis unit 1 in a matrix of 120 columns × 160 rows. The number and size of the regions 4 can be arbitrarily selected according to the purpose, and preferably 10
Adsorbent area 4 with a size less than 5 mm2
Are formed in the biochemical analysis unit 1 at a density of 10 pieces / square centimeter or more.

Further, in the above embodiment, 192
The substantially circular adsorptive region 4 having a size of about 0.01 mm 2 of 00 is regularly formed in the biochemical analysis unit 1 in a matrix of 120 columns × 160 rows. It is not always necessary to regularly form the regions 4 in the biochemical analysis unit 1.

Further, in the above-mentioned embodiment, the absorptive region 4 has a substantially circular shape, but the shape of the absorptive region 4 is not limited to the substantially circular shape, and may be arbitrarily selected. be able to.

Further, in the embodiment shown in FIGS. 1 to 13, the difference in height between the surface of the absorptive region 4 and the surface of the substrate 2, that is, the surface of the absorptive region 4 and the surface of the substrate 2 is shown. The adsorptive material is filled in the through holes 3 so that the distance between and is 5 times the diameter of the through holes 3 formed in the substrate 2,
The absorptive region 4 is formed, and in the embodiment shown in FIG. 14, the difference in height between the surface of the absorptive region 4 and the surface of the substrate 2, that is, the surface of the absorptive region 4 and the surface of the substrate 2 The adsorbent material is filled in the concave portion 15 so that the distance between the concave portion 15 and the concave portion 15 formed in the substrate 2 is three times,
Although the absorptive region 4 is formed, the height difference between the surface of the absorptive region 4 and the surface of the substrate 2, that is, the absorptive region 4 is formed.
The absorptive material is filled in the through holes 3 so that the distance between the surface of the substrate 2 and the surface of the substrate 2 is 5 times the diameter of the through holes 3 formed in the substrate 2 to form the absorptive region 4. Alternatively, it is not always necessary to fill the concave portion with the absorptive material so as to have a diameter three times the diameter of the concave portion 15 formed in the substrate 2 to form the absorptive region 4. 4 is different from the height of the surface of the substrate 2, that is, the distance between the surface of the absorptive region 4 and the surface of the substrate 2 is equal to the diameter of the through hole 3 or the recess 15 formed in the substrate 2. It may be 5 to 100 times, preferably 1 to 10 times, and within this range, the difference in height between the surface of the absorptive region 4 and the surface of the substrate 2,
That is, the distance between the surface of the absorptive region 4 and the surface of the substrate 2 can be arbitrarily selected.

Further, in the above embodiment, the stimulable phosphor sheet 10 in which the stimulable phosphor layer 12 is uniformly formed on one surface of the support 11 is provided in the biochemical analysis unit 1. The stimulable phosphor layer 12 is exposed by the radioactive labeling substance contained in the adsorptive region 4 of the biochemical analysis unit 1 in a superposed manner. A large number of holes are formed in the support 11 in the same pattern as the pattern of the through holes 3 or the recesses 15, and a large number of stimulable phosphor layers are filled with stimulable phosphors. A region for forming a stimulable phosphor sheet 10
The biochemical analysis is performed so that the surfaces of the many stimulable phosphor layer regions formed on the support 11 face the corresponding absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1. Biochemical analysis unit 1
It is also possible to expose a large number of stimulable phosphor layer regions with the radioactive labeling substance contained in the absorptive region 4 of the above.

Further, in the above-mentioned embodiment, the hybridization reaction solution 9 containing the substance of biological origin labeled with the radioactive labeling substance was prepared and contained in the many absorptive regions 4 of the biochemical analysis unit 1. The specific binding substance is hybridized with a substance derived from a living body labeled with a radioactive labeling substance, and the unit for biochemical analysis 1
Radiation data is recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1, and the radiation data recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1 is used as the support 1 of the stimulable phosphor sheet 10.
The radiation data transferred to the stimulable phosphor layer 12 formed in No. 1 and transferred to the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 by the scanner shown in FIGS. Although read and biochemical analysis data is generated, a hybridization reaction solution 9 containing a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate is prepared. The specific binding substance contained in the large number of adsorptive regions 4 of the biochemical analysis unit 1 is hybridized with a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate. Then, chemiluminescence data is recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1, and the chemiluminescence data is recorded in a large number of absorptive regions 4 of the biochemical analysis unit 1. A chemoluminescence is emitted from a large number of absorptive regions 4 of the biochemical analysis unit 1 in contact with a photo substrate, and accumulated in the biochemical analysis unit where a large number of absorptive regions 4 emit chemiluminescence. Of the stimulable phosphor layer 12 formed on the support 11 of the bioluminescent phosphor sheet 10 and the chemiluminescence emitted from the large number of adsorptive regions 4 of the biochemical analysis unit 1 allows the stimulable phosphor to be stored. Sheet 1
0 of the stimulable phosphor layer 12 is exposed to accumulate energy of chemiluminescence, and the stimulable phosphor layer 1 of the stimulable phosphor sheet 10 is stored using the scanner shown in FIGS. 6 to 13.
2 is scanned by the laser beam 24, and the stimulable light 45 emitted from the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 is irradiated.
Can be detected photoelectrically to read chemiluminescence data to generate biochemical analysis data. Even when the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 is exposed by the chemiluminescence emitted from the adsorptive region 4 of the biochemical analysis unit 1, a large number of adsorptions of the biochemical analysis unit 1 are performed. The biodegradable region 4 is formed by filling nylon 6 into the through-hole 3 formed in the substrate 2 formed of stainless steel having a property of attenuating light energy, and thus the biochemical analysis is performed. It is possible to effectively prevent the chemiluminescence emitted from the adsorptive region 4 of the biological unit 1 from being scattered in the substrate 2 of the biochemical analysis unit 1, and due to the collimation effect, a large number of through holes can be formed. It becomes possible to effectively prevent the chemiluminescence emitted from the absorptive region 4 in 3 from spreading, and therefore the stimulable phosphor contained in the stimulable phosphor layer 12 of the stimulable phosphor sheet 10 is stimulated. Sex firefly The body can be excited by the laser light 24 and photoelectrically detect the emitted photostimulable light to prevent noise from being generated in the generated biochemical analysis data. It becomes possible to generate excellent biochemical analysis data.

In the above embodiment, the substrate 2 of the biochemical analysis unit 1 is made of metal such as stainless steel, but it is made of a material having a property of attenuating radiation. The substrate 2 of the biochemical analysis unit 1 may be formed of either an inorganic compound material or an organic compound material. Particularly preferably, a metal material,
It is formed of a ceramic material or a plastic material. Examples of the inorganic compound material include gold, silver,
Copper, zinc, aluminum, titanium, tantalum, chrome,
Metals such as iron, nickel, cobalt, lead, tin and selenium;
Alloys such as brass, stainless steel and bronze; silicon materials such as silicon, amorphous silicon, glass, quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; tungsten carbide, calcium carbonate, sulfuric acid. Inorganic salts such as calcium, hydroxyapatite and gallium arsenide can be mentioned. As the organic compound material, polymer compounds are preferably used, and examples thereof include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride. Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, Nylon 6,6, Nylon such as Nylon 4, 10; Polyimide; Polysulfone; Polyphenylene sulfide Silicone resins such as polydiphenylsiloxane; Phenolic resins such as novolacs; Epoxy resins; Polyurethanes; Polystyrene Butadiene-styrene copolymers; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, and hydroxypropylmethyl cellulose; chitin; chitosan; sumac; polyamides such as gelatin, collagen and keratin, and copolymers of these polymer compounds Examples thereof include polymers.

Further, in the above embodiment, the absorptive region 4 of the biochemical analysis unit 1 is formed of nylon 6, but it is used for forming the absorptive region 4 of the biochemical analysis unit 1. The adsorptive material to be obtained is not limited to nylon 6 and other adsorptive materials can be used. As the absorptive material for forming the absorptive region 4 of the biochemical analysis unit 1, a porous material or a fibrous material is preferably used, and the biochemical analysis unit 1 is used in combination with the porous material and the fibrous material. It is also possible to form the absorptive region 4 of. The porous material used to form the adsorptive region 4 of the biochemical analysis unit 1 may be an organic material, an inorganic material, or an organic / inorganic composite. The organic porous material used to form the adsorptive region 4 of the biochemical analysis unit 1 is not particularly limited, but is a carbon porous material such as activated carbon or a porous material capable of forming a membrane filter. Materials are preferably used. Specifically, nylons such as nylon 6, nylon 6,6 and nylon 4,10; cellulose derivatives such as nitrocellulose, cellulose acetate, cellulose butyrate acetate; collagen; alginic acid, calcium alginate, alginic acid / polylysine polyion complex, etc. Examples thereof include alginic acids; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride, polyfluorides such as polytetrafluoride, and copolymers or composites thereof. The inorganic porous material used to form the adsorptive region 4 of the biochemical analysis unit 1 is not particularly limited, but preferably, for example, platinum, gold, iron, silver, nickel, aluminum. Metals such as; alumina, silica,
Examples thereof include metal oxides such as titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and composites thereof. The fibrous material used to form the adsorptive region 4 of the biochemical analysis unit 1 is not particularly limited, but preferably, for example, nylon 6, nylon 6,6, nylon 4,10, etc. Examples thereof include nylons, cellulose derivatives such as nitrocellulose, cellulose acetate, and cellulose acetate butyrate.

[0185]

EFFECTS OF THE INVENTION According to the present invention, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. are formed. The biochemical analysis unit obtained by forming a plurality of spot-shaped regions on the carrier surface with high density and selectively labeling the multiple spot-shaped regions with a radioactive labeling substance is brought into close contact with the stimulable phosphor layer to produce a luminescent material. Exposing the exhaustive phosphor layer with a radioactive labeling substance,
Irradiating the stimulable phosphor layer with excitation light and photoelectrically detecting the stimulable light emitted from the stimulable phosphor layer to generate biochemical analysis data and analyze substances derived from living organisms. Even when
Exposure of biochemical analysis unit and photostimulable phosphor layer capable of preventing noise caused by scattering of electron beams (β-rays) emitted from radiolabeled substances in biochemical analysis data It becomes possible to provide a method.

Further, according to the present invention, a plurality of spot-like regions containing a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition and the like are formed. , A biochemical analysis unit obtained by selectively labeling with a labeling substance that forms chemiluminescence by contacting a chemiluminescent substrate with a plurality of spot-shaped regions formed on a carrier surface at high density, A chemiluminescent substrate is brought into contact to selectively emit chemiluminescence from a plurality of spot-shaped regions, and a biochemical analysis unit in which chemiluminescence is selectively emitted from a plurality of spot-shaped regions is illuminated. The photostimulable phosphor layer is brought into close contact with the photostimulable phosphor layer, and the photostimulable phosphor layer is exposed by chemiluminescence. Photoelectrically detected for biochemical analysis data Even when generating and analyzing substances of biological origin, noise due to scattering of chemiluminescence emitted from multiple spot-like regions of the biochemical analysis unit is generated in the biochemical analysis data. It becomes possible to provide a biochemical analysis unit and a method for exposing a stimulable phosphor layer that can be prevented.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a schematic cross-sectional view of a biochemical analysis unit.

FIG. 3 is a schematic front view of a spotting device.

FIG. 4 is a schematic cross-sectional view of a hybridization reaction container.

FIG. 5 is a schematic cross-sectional view showing a method for exposing a stimulable phosphor layer formed on a stimulable phosphor sheet with a radioactive labeling substance contained in a large number of adsorptive regions.

FIG. 6 is a schematic perspective view showing an example of a scanner.

FIG. 7 is a schematic perspective view showing details near the photomultiplier of the scanner.

8 is a schematic cross-sectional view taken along the line AA of FIG.

9 is a cross-sectional view taken along the line BB of FIG.

10 is a cross-sectional view taken along the line CC of FIG.

11 is a cross-sectional view taken along the line DD of FIG.

FIG. 12 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 13 is a block diagram showing a control system, an input system and a drive system of the scanner shown in FIG.

FIG. 14 is a schematic sectional view of a biochemical analysis unit according to another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical analysis unit 2 substrates 3 through holes 4 Adsorbable area 5 Spotting device 6 injectors 7 CCD camera 8 Hybridization reaction container 9 Hybridization reaction solution 10 Storage phosphor sheet 11 Support 12 Photostimulable phosphor layer 15 recess 21 First laser excitation light source 22 Second laser excitation light source 23 Third Laser Excitation Light Source 24 laser light 25 Collimator lens 26 mirror 27 First Dichroic Mirror 28 Second dichroic mirror 29 mirror 30 collimator lens 31 Collimator lens 32 mirror 33 holes for the perforated mirror 34 perforated mirror 35 Optical head 36 mirror 37 Aspherical lens 38 concave mirror 40 stages 41 glass plate 45 Fluorescence or stimulated emission 48 filter units 50 Photomultiplier 51a, 51b, 51c, 51d filter member 52a, 52b, 52c, 52d filters 53 A / D converter 54 Data processing device 60 substrates 61 Sub-scanning pulse motor 62 a pair of rails 63 Movable substrate 64 rod 65 Main scanning pulse motor 66 endless belt 67 Linear encoder 70 Control unit 71 keyboard 72 Filter unit motor

Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01T 1/00 G01T 1/00 B 1/29 1/29 D G21K 4/00 G21K 4/00 LF term (reference) 2G054 AA06 EA03 FB01 2G083 AA03 AA09 BB03 CC10 EE10 2G088 EE27 FF05 GG10 GG25 HH08 JJ30 KK32 KK35 LL09 LL11 LL12 4B029 AA07 AA08 BB16 BB17 BB20 CC04 FA12 GB06 GA09 GB04 GB03

Claims (34)

[Claims] 1. A plurality of absorptive regions formed of an absorptive material and spaced apart from each other, and radiation and / or
Or a plurality of separating portions formed of a material having a property of attenuating light and separating the plurality of absorptive regions, and the surfaces of the plurality of separating portions respectively project from the surface of the absorptive region. As described above, the unit for biochemical analysis, wherein the plurality of separation portions are formed. 2. The substrate having a plurality of absorptive regions of the biochemical analysis unit formed of a material having a property of attenuating radiation and / or light, and a plurality of holes formed apart from each other. The biochemical analysis unit according to claim 1, wherein the biochemical analysis unit is formed by being filled with an adsorptive material, and the plurality of separation portions are constituted by the substrate. 3. A substrate, in which the plurality of absorptive regions are formed of a material that attenuates radiation and / or light, is filled with an absorptive material in a plurality of through holes formed apart from each other. 3. The biochemical analysis unit according to claim 2, wherein the plurality of separation parts are formed by the substrate. 4. A plurality of recesses formed in the substrate, in which the plurality of absorptive regions of the biochemical analysis unit are spaced apart from each other, on a substrate formed of a material having a property of attenuating radiation and / or light. The biochemical analysis unit according to claim 2, wherein the biochemical analysis unit is formed by filling an adsorbent material with the substrate, and the plurality of separation portions are formed of the substrate. 5. The surfaces of the plurality of separation parts are respectively
2. The plurality of separating portions are formed so as to protrude from the surface of the absorptive region by 0.5 to 100 times the maximum width of the absorptive region.
5. The biochemical analysis unit according to any one of 4 to 4. 6. The surface of each of the plurality of separating portions is
The biochemistry according to claim 5, wherein the plurality of separation portions are formed so as to protrude from the surface of the absorptive region by 1 to 10 times the maximum width of the absorptive region. Analysis unit. 7. The biochemical analysis unit according to claim 1, wherein 10 or more absorptive regions are formed in the biochemical analysis unit. 8. The biochemical analysis unit comprises 1000
The biochemical analysis unit according to any one of claims 1 to 7, wherein at least one absorptive region is formed. 9. The biochemical analysis unit comprises 1000 units.
The biochemical analysis unit according to claim 8, wherein 0 or more absorptive regions are formed. 10. The plurality of absorptive regions formed in the biochemical analysis unit each have a size of less than 5 square millimeters. The unit for biochemical analysis according to the item. 11. The biochemical analysis according to claim 10, wherein each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 1 mm 2. Unit. 12. The raw material according to claim 11, wherein each of the plurality of absorptive regions formed in the biochemical analysis unit has a size of less than 0.1 mm 2. Chemical analysis unit. 13. The biochemical analysis unit, wherein the plurality of absorptive regions are formed at a density of 10 pieces / square centimeter.
The biochemical analysis unit according to any one of 2 above. 14. The biochemical analysis unit according to claim 13, wherein the plurality of absorptive regions are formed in the biochemical analysis unit at a density of 1000 pieces / square centimeter. 15. The biochemical analysis unit is formed with the plurality of absorptive regions at a density of 10000 pieces / square centimeter.
The biochemical analysis unit described in. 16. The radiation and / or light when the separation section of the biochemical analysis unit transmits radiation and / or light through the separation section by a distance equal to a distance between adjacent absorptive regions. Or the energy of light is 1 /
The biochemical analysis unit according to any one of claims 1 to 15, which has a property of being attenuated to 5 or less. 17. The separation portion of the biochemical analysis unit has a distance equal to a distance between adjacent absorptive regions,
When the radiation and / or the light passes through the separation part, the energy of the radiation and / or the light is reduced to 1/10.
The biochemical analysis unit according to claim 16, which has the property of being attenuated as follows. 18. The separation section of the biochemical analysis unit has a distance equal to the distance between adjacent absorptive regions,
When the radiation and / or the light passes through the separation part, the energy of the radiation and / or the light is reduced to 1/10.
The biochemical analysis unit according to claim 17, which has a property of being attenuated to 0 or less. 19. The separation section of the biochemical analysis unit is formed of a material selected from the group consisting of a metal material, a ceramic material, and a plastic material. The unit for biochemical analysis according to the item. 20. The biochemical analysis unit according to claim 19, wherein the separation portion of the biochemical analysis unit is formed of a metal material. 21. The biochemistry according to claim 1, wherein the plurality of absorptive regions of the biochemical analysis unit are formed of a porous material or a fibrous material. Analysis unit. 22. A plurality of absorptive regions formed of an absorptive material and separated from each other, and a plurality of separations formed of a material having a property of attenuating radiation to separate the plurality of absorptive regions. And a surface of each of the plurality of separation parts, so that each of the plurality of separation parts is formed so as to protrude from the surface of the absorptive region, in the plurality of absorptive regions of the biochemical analysis unit in which the plurality of separation parts are formed. , A specific binding substance having a known structure or characteristics is dropped, and the plurality of adsorptive regions are selectively labeled with a radiolabeling substance to generate a biochemical analysis unit, and the biochemical analysis unit is , The plurality of separating portions are in contact with the surface of the stimulable phosphor layer formed on the stimulable phosphor sheet,
The stimulable phosphor is superposed on the stimulable phosphor layer of the stimulable phosphor sheet by a radioactive labeling substance selectively contained in the plurality of absorptive regions of the biochemical analysis unit. A method for exposing a stimulable phosphor layer, which comprises exposing the stimulable phosphor layer of a sheet. 23. The absorptive material is provided in a plurality of holes formed in a substrate, in which the plurality of absorptive regions of the biochemical analysis unit are formed of a material having a property of attenuating radiation. 23. The method for exposing a stimulable phosphor layer according to claim 22, wherein the plurality of separating portions are formed by filling the substrate with the stimulable phosphor layer. 24. A plurality of absorptive regions formed of an absorptive material and separated from each other, and a plurality of separations formed of a material having a property of attenuating radiation to separate the plurality of absorptive regions. And a surface of each of the plurality of separation parts, so that each of the plurality of separation parts is formed so as to protrude from the surface of the absorptive region, in the plurality of absorptive regions of the biochemical analysis unit in which the plurality of separation parts are formed. , A specific binding substance having a known structure or property is dropped, and the plurality of absorptive regions are selectively labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate for biochemical analysis. A unit is generated, and a chemiluminescent substrate is brought into contact with the plurality of absorptive regions of the biochemical analysis unit so that chemiluminescence is emitted from the plurality of absorptive regions, and the plurality of adsorbents are adsorbed. From the active region, the biochemical analysis unit that has emitted chemiluminescence, the plurality of separation portions, so as to contact the surface of the stimulable phosphor layer formed in the stimulable phosphor sheet, Overlaid on the stimulable phosphor layer of the stimulable phosphor sheet, from the plurality of absorptive regions of the biochemical analysis unit, by chemiluminescence selectively released, the stimulable phosphor sheet of the. A method for exposing a stimulable phosphor layer, which comprises exposing the stimulable phosphor layer. 25. An adsorbent material is provided in a plurality of holes formed in a substrate, in which the plurality of absorptive regions of the biochemical analysis unit are formed of a material having a property of attenuating light. 25. The method of exposing a stimulable phosphor layer according to claim 24, characterized in that the plurality of separation portions are formed by filling the substrate with the substrate. 26. The surfaces of the plurality of separation parts of the biochemical analysis unit are each projected from the surface of the absorptive region by 0.5 to 100 times the maximum width of the absorptive region. 26. The method of exposing a stimulable phosphor layer according to claim 22, wherein the plurality of separation portions are formed on the substrate. 27. The surfaces of the plurality of separation portions of the biochemical analysis unit are each projected from the surface of the absorptive region by 1 to 10 times the maximum width of the absorptive region, 27. The method of exposing a stimulable phosphor layer according to claim 26, wherein the plurality of separation portions are formed. 28. The photostimulable phosphor layer according to claim 22, wherein at least 10 absorptive regions are formed in the biochemical analysis unit. Exposure method. 29. The absorptive region formed in the biochemical analysis unit has a size of less than 5 mm 2 respectively. The method for exposing a stimulable phosphor layer according to the item 1. 30. The biochemical analysis unit, wherein the plurality of absorptive regions are formed at a density of 10 pieces / square centimeter, according to any one of claims 22 to 29. Method for exposing photostimulable phosphor layer. 31. The energy of radiation when the separating section of the biochemical analysis unit transmits radiation and / or light through the separating section by a distance equal to the distance between adjacent absorptive regions. 22 has a property of attenuating to 1/5 or less.
0. The method for exposing a stimulable phosphor layer according to any one of 0. 32. The photostimulability according to claim 31, wherein the separation portion of the biochemical analysis unit is formed of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. Method of exposing phosphor layer. 33. The method of exposing a stimulable phosphor layer according to claim 32, wherein the separation portion of the biochemical analysis unit is formed of a metal material. 34. The adsorbent material is formed of a porous material or a fibrous material.
34. The method for exposing a stimulable phosphor layer according to any one of 2 to 33.