JP2002325568A - Novel microorganism belonging to bacillus cereus and method of deodorization by using the same - Google Patents
Novel microorganism belonging to bacillus cereus and method of deodorization by using the sameInfo
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- JP2002325568A JP2002325568A JP2002017851A JP2002017851A JP2002325568A JP 2002325568 A JP2002325568 A JP 2002325568A JP 2002017851 A JP2002017851 A JP 2002017851A JP 2002017851 A JP2002017851 A JP 2002017851A JP 2002325568 A JP2002325568 A JP 2002325568A
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Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、バチルス・セレウス種
に属する新規微生物およびそれを用いた脱臭方法に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel microorganism belonging to the species Bacillus cereus and a method for deodorizing the microorganism.
【０００２】[0002]
【従来の技術】バチルス・セレウス種に属する菌として
は、食品腐敗菌や食中毒菌の研究が進んでいるが、有用
微生物としての研究は全く行われていないといっても過
言ではない。2. Description of the Related Art As bacteria belonging to the genus Bacillus cereus, research on food spoilage bacteria and food poisoning bacteria has been advanced, but it is no exaggeration to say that no research on useful microorganisms has been conducted.
【０００３】[0003]
【発明が解決しようとする課題】そこで、本発明は、バ
チルス・セレウス種に属する有用新規微生物、およびそ
れを用いた脱臭方法を提供することを目的とするもので
ある。Accordingly, an object of the present invention is to provide a useful novel microorganism belonging to the species Bacillus cereus, and a method for deodorizing using the microorganism.
【０００４】[0004]
【課題を解決するための手段】上記の課題は、本発明の
下記（１）または（２）の構成により達成される。 （１） 下記の菌学的特徴を有するバチルス・セレウス
種に属する新規微生物。 １．形態 ：桿菌 ２．グラム染色性 ：＋ ３．胞子 ：＋ 形 ：楕円形 位置 ：端立〜亜端立 胞子嚢 ：非膨出 ４．運動性 ：＋ ５．酸素に対する態度 ：通性嫌気性 ６．カタラーゼ ：＋ ７．嫌気下での成育 ：＋ ８．Ｖ−Ｐ反応 ：＋ ９．Ｖ−ＰブロスのｐＨ ：４．８ １０．グリコースからの酸の生成 ：＋ １１．グリコースからのガスの生成 ：− １２．ゼラチンの液化 ：＋ １３．デンプンの分解 ：− １４．クエン酸塩の利用 ：＋ １５．プロピオン酸塩の利用 ：＋ １６．卵黄反応 ：＋ １７．硝酸塩の還元 ：＋ １８．ｐＨ６．８での成育 ：＋ （ニュートリエントブロス） １９．ｐＨ５．７での成育 ：＋ ２０．５％Ｎａｃｌ存在下での成育 ：＋ ２１．７％Ｎａｃｌ存在下での成育 ：＋ ２２．１０℃での成育 ：− ２３．３０℃での成育 ：＋ ２４．４０℃での成育 ：＋ ２５．５０℃での成育 ：− ２６．菌体内ＤＮＡのＧＣ含量 ：３５mol％ （ＨＰＬＣ法による） （２） 上記（１）のバチルス・セレウス種に属する新
規微生物を発臭源に散布することにより脱臭を行う脱臭
方法。The above object is achieved by the present invention which is defined below as (1) or (2). (1) A novel microorganism belonging to the species Bacillus cereus having the following mycological characteristics. 1. Form: Bacillus 2. Gram stainability: +3. 3. Spores: + Shape: Oval Position: Erect-subepithelial Spores: Non-bulging Motility: +5. 5. Attitude to oxygen: facultative anaerobic Catalase: +7. Growth under anaerobic: +8. VP reaction: +9. 10. pH of VP broth: 4.8 10. Generation of acid from glucose: +11. Production of gas from glucose: -12. Liquefaction of gelatin: +13. Decomposition of starch: -14. Use of citrate: +15. Use of propionate: +16. Egg yolk reaction: +17. Nitrate reduction: +18. 18. Growth at pH 6.8: + (nutrient broth) Growth at pH 5.7: + Growth in the presence of 20.5% NaCl: + Growth in the presence of 21.7% NaCl: + Growth at 22.10 ° C .: Growth at −23.30 ° C .: + 24. Growth at 40 ° C .: + 25.50 ° C. Growth: −26. GC content of intracellular DNA: 35 mol% (by HPLC method) (2) A deodorization method in which the novel microorganism belonging to the Bacillus cereus species of (1) is sprayed to an odor source to deodorize.
【０００５】[0005]
【発明の実施の形態】本発明によるバチルス・セレウス
種に属する新規微生物は、Bacillus cereusCCC-b（寄託
番号：FERM P-16176）として独立行政法人産業技術総合
研究所特許生物寄託センターに寄託されている。DESCRIPTION OF THE PREFERRED EMBODIMENTS A novel microorganism belonging to the genus Bacillus cereus according to the present invention has been deposited as Bacillus cereus CCC-b (deposit number: FERM P-16176) with the Patent Organism Depositary of the National Institute of Advanced Industrial Science and Technology. I have.
【０００６】以下、このBacillus cereus CCC-bの菌学
的性質を説明する。Hereinafter, the mycological properties of the Bacillus cereus CCC-b will be described.
【０００７】 １．形態 ：桿菌 ２．グラム染色性 ：＋ ３．胞子 ：＋ 形 ：楕円形 位置 ：端立〜亜端立 胞子嚢 ：非膨出 ４．運動性 ：＋ ５．酸素に対する態度 ：通性嫌気性 ６．カタラーゼ ：＋ ７．嫌気下での成育 ：＋ ８．Ｖ−Ｐ反応 ：＋ ９．Ｖ−ＰブロスのｐＨ ：４．８ １０．グリコースからの酸の生成 ：＋ １１．グリコースからのガスの生成 ：− １２．ゼラチンの液化 ：＋ １３．デンプンの分解 ：− １４．クエン酸塩の利用 ：＋ １５．プロピオン酸塩の利用 ：＋ １６．卵黄反応 ：＋ １７．硝酸塩の還元 ：＋ １８．ｐＨ６．８での成育 ：＋ （ニュートリエントブロス） １９．ｐＨ５．７での成育 ：＋ ２０．５％Ｎａｃｌ存在下での成育 ：＋ ２１．７％Ｎａｃｌ存在下での成育 ：＋ ２２．１０℃での成育 ：− ２３．３０℃での成育 ：＋ ２４．４０℃での成育 ：＋ ２５．５０℃での成育 ：− ２６．菌体内ＤＮＡのＧＣ含量 ：３５mol％ （ＨＰＬＣ法による）[0007] 1. Form: Bacillus 2. Gram stainability: +3. 3. Spores: + Shape: Oval Position: Erect-subepithelial Spores: Non-bulging Motility: +5. 5. Attitude to oxygen: facultative anaerobic Catalase: +7. Growth under anaerobic: +8. VP reaction: +9. 10. pH of VP broth: 4.8 10. Generation of acid from glucose: +11. Production of gas from glucose: -12. Liquefaction of gelatin: +13. Decomposition of starch: -14. Use of citrate: +15. Use of propionate: +16. Egg yolk reaction: +17. Nitrate reduction: +18. 18. Growth at pH 6.8: + (nutrient broth) Growth at pH 5.7: + Growth in the presence of 20.5% NaCl: + Growth in the presence of 21.7% NaCl: + Growth at 22.10 ° C .: Growth at −23.30 ° C .: + 24. Growth at 40 ° C .: + 25.50 ° C. Growth: −26. GC content of intracellular DNA: 35 mol% (by HPLC method)
【０００８】以上の菌学的性質の全体から、本菌は、バ
チルス属に属し、通性嫌気性であり、卵黄反応：＋から
バチルス・セレウス種に属する菌であると判定したが、
このBacillus cereus CCC-bは、デンプンの分解を行わ
ないという非典型性状を示したので新菌であると判定し
た。このBacillus cereus CCC-bは、一般栄養培地であ
るならいかなる培地でも良好に成育する。[0008] From the overall mycological properties described above, the bacterium belongs to the genus Bacillus, is facultatively anaerobic, and is determined to be a bacterium belonging to the genus Bacillus cereus from the yolk reaction: +
This Bacillus cereus CCC-b exhibited an atypical property of not degrading starch, and thus was determined to be a new bacterium. The Bacillus cereus CCC-b grows well on any general nutrient medium.
【０００９】培養は、好ましくは１５〜４５℃程度で良
好に増殖する。The culture preferably grows well at about 15 to 45 ° C.
【００１０】このBacillus cereus CCC-bは、水や液体
培地に分散させ分散液の状態で各種用途に用いることが
できるが、好ましくは微生物群を担体に固定し、取扱を
容易にした担体固定化微生物群として用いる。This Bacillus cereus CCC-b can be dispersed in water or a liquid medium and used in various forms in the form of a dispersion. Preferably, the microorganisms are immobilized on a carrier, and the carrier is immobilized to facilitate handling. Use as a group of microorganisms.
【００１１】微生物群を固定する担体としては、微生物
の保持容量が大きく、また微生物の活性化が容易である
ことが好ましい。It is preferable that the carrier for immobilizing the microorganisms has a large holding capacity for the microorganisms and easily activates the microorganisms.
【００１２】担体としては、岩石（例えば、真珠岩、珪
藻土）またはその粉砕物、砂利、砂、プラスチックス、
セラミックス（例えば、アルミナ、シリカ、天然ゼオラ
イト、合成ゼオライト）、タルク等も好ましく、特に、
多孔質セラミックス、多孔質プラスチックスのような連
続通気孔を有する多孔質材料が好ましい。例えば、真珠
岩の粉砕物を高温高圧で保持し熱処理した後、急激に減
圧して多孔質にした微細粉（商品名：パーライト）は、
菌類を着生させる性質があるため、好適である。このよ
うな多孔質材料において、通気孔の径は、２〜１０μｍ
程度であることが好ましい。また、このような担体の性
状としては、塊状物、粒体、粉体、微粉末または板状
物、針状物等いかなるものでもよいが、好ましくは平均
粒径が２ｍｍ以下、特に５０μｍ〜１ｍｍ程度の粉粒体
がよい。なお、このような担体固定化微生物群は、必要
に応じて、布やネット等の通水性や通気性のよい容器に
収納して使用しても良い。As the carrier, rock (eg, perlite, diatomaceous earth) or its crushed material, gravel, sand, plastics,
Ceramics (eg, alumina, silica, natural zeolites, synthetic zeolites), talc, and the like are also preferable.
A porous material having continuous air holes such as porous ceramics and porous plastics is preferable. For example, a fine powder (trade name: pearlite), which is obtained by holding a pearlite pulverized material at high temperature and pressure and heat-treating it and then rapidly reducing the pressure to make it porous,
It is suitable because it has a property of growing fungi. In such a porous material, the diameter of the vent is 2 to 10 μm.
It is preferred that it is about. In addition, the properties of such a carrier may be any such as a lump, a granule, a powder, a fine powder or a plate, and a needle, but preferably have an average particle diameter of 2 mm or less, particularly 50 μm to 1 mm. Good granularity is good. In addition, such a carrier-immobilized microorganism group may be stored in a water-permeable or air-permeable container such as a cloth or a net, and used, if necessary.
【００１３】上記担体としては、これらの他、リボン状
やシート状の織布や不織布を用いることができる。[0013] In addition to the above, a ribbon-shaped or sheet-shaped woven or non-woven fabric can be used as the carrier.
【００１４】このような担体に微生物群を担持させるに
は、担体と本発明の微生物群分散液とを混合した後、乾
燥すればよいが、担体で直接微生物群を培養してもよ
い。To support the microorganisms on such a carrier, the carrier and the dispersion of the microorganisms of the present invention may be mixed and then dried, but the microorganisms may be directly cultured on the carrier.
【００１５】担体の微生物群の担持量は、担持させると
きの条件によっても異なるが、好ましくは、５個〜２０
０億個／ｃｍ３、好ましくは１０個〜１００億個／ｃｍ
３である。The amount of microorganisms to be carried on the carrier varies depending on the conditions at which the microorganisms are carried.
100 million / cm 3 , preferably 10 to 10 billion / cm 3
3 .
【００１６】このBacillus cereus CCC-bを用いること
により・養豚所、公衆便所等に用いて、脱臭効果が確認
された。また、このBacillus cereus CCC-bは、排水中
の有機物を有効に分解しえ、更には、リン等の金属の回
収機能が確認された。By using this Bacillus cereus CCC-b, its deodorizing effect was confirmed in pig farms and public toilets. In addition, this Bacillus cereus CCC-b was able to effectively decompose organic matter in wastewater, and furthermore, was confirmed to have a function of recovering metals such as phosphorus.
【００１７】なお、上記Bacillus cereus CCC-bは、マ
ウスによる経口投与試験によりその安全性が確認され
た。The safety of Bacillus cereus CCC-b was confirmed by an oral administration test using mice.
【００１８】[0018]
【実施例】次に、本発明の実施例について説明する。ま
ず、次のような組成の培地を作成した。 培地 ペプトン ５ｇ 肉エキス ５ｇ Ｎａｃｌ ２．５ｇ 水 ５００ｍｌ（ｐＨ７．０） 以上の組成の培地を、１０００ｍｌ容器に入れ、これに
Bacillus cereus CCC-bを接種し、よく撹拌した後、静
置して、３０℃で１日間培養した。得られた培養液を遠
心分離機にかけ、菌体を分離し、これをよく洗浄し、担
体に付着させてパウダーとした。担体としては珪藻土を
焼いて、粉砕したものを用いた。Next, an embodiment of the present invention will be described. First, a medium having the following composition was prepared. Medium Peptone 5 g Meat extract 5 g NaCl 2.5 g Water 500 ml (pH 7.0) A medium having the above composition is put into a 1000 ml container, and
Bacillus cereus CCC-b was inoculated, stirred well, allowed to stand, and cultured at 30 ° C. for 1 day. The obtained culture was centrifuged to separate the cells, which were thoroughly washed and adhered to a carrier to obtain a powder. As the carrier, diatomaceous earth was burned and pulverized.
【００１９】このパウダー状のBacillus cereus CCC-b
を、１本のままの腐った鰯に振りかけ２時間経過後にそ
の臭いをかいだところ、明かに臭気が減少していた。参考実験 上記パウダー状のBacillus cereus CCC-bを、計量対象
へ接種してのＣＯＤ、全窒素量および全リン量の経時変
化を計量した。試験方法は、ＣＯＤはＪＩＳ０１０２−
１７、全窒素量はＪＩＳ ０１０２−４５．２、そして
全リン量はＪＩＳ ０１０２−４６．２に従って行っ
た。計量対象としては、海水での牛乳１００倍液に、１
リットル当たり０．２ｇの澱粉を加えたものを用いた。
そのままのものを比較例とし、これにBacillus cereus
CCC-bを接種したものを実施例とした。試験は、２０℃
に保って行った。また、各量の測定にあたっては、計量
対象液を２時間静置し、その上澄み液を試験液とした。
試験の結果を表１に示した。なお、試験は、それぞれサ
ンプルを２つずつ作製し、その平均を測定値とした。This powdery Bacillus cereus CCC-b
Sprinkle on the rotten sardine as it is, and after 2 hours,
When the odor was smelled, the odor was clearly reduced.Reference experiment Bacillus cereus CCC-b in powder form
Change of COD, total nitrogen and total phosphorus after inoculation
Was measured. The test method is JIS0102-COD.
17, total nitrogen amount is JIS 0102-45.2, and
The total phosphorus amount was determined according to JIS 0102-46.2.
Was. As a measurement target, 100 times milk in seawater
What added 0.2 g of starch per liter was used.
Bacillus cereus was used as a comparative example.
The example inoculated with CCC-b was used as an example. Test at 20 ° C
I kept it. Also, when measuring each quantity,
The target solution was allowed to stand for 2 hours, and the supernatant was used as a test solution.
Table 1 shows the results of the test. The tests were
Two samples were prepared, and the average was used as the measured value.
【００２０】[0020]
【表１】 [Table 1]
【００２１】表１から分かるように、Bacillus cereus
CCC-bは、水の浄化機能を有し、金属を除去する作用が
あることが判明した。このように、Bacillus cereus CC
C-bは、上記の実験で確認された脱臭機能、有機物分解
機能、金属除去機能を備えた極めて有用な新規微生物で
ある。As can be seen from Table 1, Bacillus cereus
It turned out that CCC-b has a water purification function and has an action of removing metals. Thus, Bacillus cereus CC
Cb is a very useful novel microorganism having a deodorizing function, an organic matter decomposing function, and a metal removing function confirmed in the above experiment.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ テーマコート゛(参考） // Ｃ０２Ｆ 3/34 ＺＡＢ Ｃ１２Ｒ 1:085 (Ｃ１２Ｎ 1/20 Ｂ０９Ｂ 3/00 Ａ Ｃ１２Ｒ 1:085） Ｆターム(参考） 4B065 AA16X AC20 BA22 BC42 BD14 CA56 4C080 AA07 BB02 CC14 HH05 JJ04 KK06 LL10 MM33 4D004 AA03 CA18 CA48 CC07 4D040 DD01 ──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. 7 Identification symbol FI Theme coat ゛ (Reference) // C02F 3/34 ZAB C12R 1: 085 (C12N 1/20 B09B 3/00 A C12R 1: 085) F Term (reference) 4B065 AA16X AC20 BA22 BC42 BD14 CA56 4C080 AA07 BB02 CC14 HH05 JJ04 KK06 LL10 MM33 4D004 AA03 CA18 CA48 CC07 4D040 DD01
Claims (2)
レウス種に属する新規微生物。 １．形態 ：桿菌 ２．グラム染色性 ：＋ ３．胞子 ：＋ 形 ：楕円形 位置 ：端立〜亜端立 胞子嚢 ：非膨出 ４．運動性 ：＋ ５．酸素に対する態度 ：通性嫌気性 ６．カタラーゼ ：＋ ７．嫌気下での成育 ：＋ ８．Ｖ−Ｐ反応 ：＋ ９．Ｖ−ＰブロスのｐＨ ：４．８ １０．グリコースからの酸の生成 ：＋ １１．グリコースからのガスの生成 ：− １２．ゼラチンの液化 ：＋ １３．デンプンの分解 ：− １４．クエン酸塩の利用 ：＋ １５．プロピオン酸塩の利用 ：＋ １６．卵黄反応 ：＋ １７．硝酸塩の還元 ：＋ １８．ｐＨ６．８での成育 ：＋ （ニュートリエントブロス） １９．ｐＨ５．７での成育 ：＋ ２０．５％Ｎａｃｌ存在下での成育 ：＋ ２１．７％Ｎａｃｌ存在下での成育 ：＋ ２２．１０℃での成育 ：− ２３．３０℃での成育 ：＋ ２４．４０℃での成育 ：＋ ２５．５０℃での成育 ：− ２６．菌体内ＤＮＡのＧＣ含量 ：３５mol％ （ＨＰＬＣ法による）1. A novel microorganism belonging to the species Bacillus cereus having the following mycological characteristics: 1. Form: Bacillus 2. Gram stainability: +3. 3. Spores: + Shape: Oval Position: Erect-subepithelial Spores: Non-bulging Motility: +5. 5. Attitude to oxygen: facultative anaerobic Catalase: +7. Growth under anaerobic: +8. VP reaction: +9. 10. pH of VP broth: 4.8 10. Generation of acid from glucose: +11. Production of gas from glucose: -12. Liquefaction of gelatin: +13. Decomposition of starch: -14. Use of citrate: +15. Use of propionate: +16. Egg yolk reaction: +17. Nitrate reduction: +18. 18. Growth at pH 6.8: + (nutrient broth) Growth at pH 5.7: + Growth in the presence of 20.5% NaCl: + Growth in the presence of 21.7% NaCl: + Growth at 22.10 ° C .: Growth at −23.30 ° C .: + 24. Growth at 40 ° C .: + 25.50 ° C. Growth: −26. GC content of intracellular DNA: 35 mol% (by HPLC method)
る新規微生物を発臭源に散布することにより脱臭を行う
脱臭方法。2. A method for deodorizing a novel microorganism belonging to the Bacillus cereus species according to claim 1 by spraying the microorganism on an odor source.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002017851A JP3748822B2 (en) | 2002-01-28 | 2002-01-28 | Novel microorganism belonging to Bacillus cereus species and deodorizing method using the same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002017851A JP3748822B2 (en) | 2002-01-28 | 2002-01-28 | Novel microorganism belonging to Bacillus cereus species and deodorizing method using the same |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12015897A Division JP3650687B2 (en) | 1997-04-23 | 1997-04-23 | Wastewater treatment method |
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| Publication Number | Publication Date |
|---|---|
| JP2002325568A true JP2002325568A (en) | 2002-11-12 |
| JP3748822B2 JP3748822B2 (en) | 2006-02-22 |
Family
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| JP2002017851A Expired - Lifetime JP3748822B2 (en) | 2002-01-28 | 2002-01-28 | Novel microorganism belonging to Bacillus cereus species and deodorizing method using the same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106424087A (en) * | 2016-10-18 | 2017-02-22 | 桂林洁宇环保科技有限责任公司 | Application of anaerobic spore-bearing bacilli to household refuse anaerobic treatment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106424087A (en) * | 2016-10-18 | 2017-02-22 | 桂林洁宇环保科技有限责任公司 | Application of anaerobic spore-bearing bacilli to household refuse anaerobic treatment |
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| JP3748822B2 (en) | 2006-02-22 |
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