JP3650687B2 - Wastewater treatment method - Google Patents
Wastewater treatment method Download PDFInfo
- Publication number
- JP3650687B2 JP3650687B2 JP12015897A JP12015897A JP3650687B2 JP 3650687 B2 JP3650687 B2 JP 3650687B2 JP 12015897 A JP12015897 A JP 12015897A JP 12015897 A JP12015897 A JP 12015897A JP 3650687 B2 JP3650687 B2 JP 3650687B2
- Authority
- JP
- Japan
- Prior art keywords
- ccc
- bacillus cereus
- carrier
- wastewater treatment
- treatment method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Description
【0001】
【産業上の利用分野】
本発明は、排水処理方法に関し、更に詳細には、微生物を用いた排水処理方法に関する。
【0002】
【従来の技術】
排水浄化菌としては、各種のバチルス属微生物が知られている。しかしながら、これまでに排水処理に用いられていたバチルス属微生物としては、排水中の有機物の除去および脱臭を行なうものは知られているが、同時にリン成分の除去をも行なえる微生物については何ら報告がない。
【0003】
【発明が解決しようとする課題】
そこで、本発明は、排水からの有機物の除去および脱臭とともに、リン除去もできる微生物を用いた排水処理方法を提供することを目的とするものである。
【0004】
【課題を解決するための手段】
本発明者らは、上記のような要素を全て満足することのできる微生物を求めて鋭意探索したところ、バチルス・セレウス(Bacillus cereus) CCC−b(寄託番号FERM P−16176)により排水の有機物の除去、脱臭、リン除去の全てを行なえることを知見した。
【0005】
本発明は、この知見に基づくものであり、バチルス・セレウスCCC c-bを排水に接触させることにより、排水の有機物の除去、脱臭、リン除去を行なうことを特徴とするものである。
【0006】
上記バチルス・セレウスCCC -b は、 Bacillus cereus CCC − b (寄託番号 FERM P −16176)として独立行政法人産業技術総合研究所特許生物寄託センターに寄託されている。
【0007】
以下、このBacillus
cereus CCC − bの菌学的性質を説明する。
【0008】
【0009】
このバチルス・セレウス CCC -bは、デンプンの分解を行なわないという非典型性状を示した。また、このバチルス・セレウス CCC -b、一般栄養培地であるならいかなる培地でも良好に成育する。
【0010】
培養は、好ましくは15〜45℃程度で良好に増殖する。
【0011】
バチルス・セレウスは、水や液体培地に分散させ分散液の状態で各種用途に用いることができるが、好ましくは微生物群を担体に固定し、取扱を容易にした担体固定化微生物群として用いる。
【0012】
微生物群を固定する担体としては、微生物の保持容量が大きく、また微生物の活性化が容易であることが好ましい。
【0013】
担体としては、岩石(例えば、真珠岩、珪藻土)またはその粉砕物、砂利、砂、プラスチックス、セラミックス(例えば、アルミナ、シリカ、天然ゼオライト、合成ゼオライト)、タルク等も好ましく、特に、多孔質セラミックス、多孔質プラスチックスのような連続通気孔を有する多孔質材料が好ましい。例えば、真珠岩の粉砕物を高温高圧で保持し熱処理した後、急激に減圧して多孔質にした微細粉(商品名:パーライト)は、菌類を着生させる性質があるため、好適である。このような多孔質材料において、通気孔の径は、2〜10μm程度であることが好ましい。また、このような担体の性状としては、塊状物、粒体、粉体、微粉末またはいた状物、針状物等いかなるものでもよいが、好ましくは平均粒径が2mm以下、特に50μm〜1mm程度の粉粒体がよい。なお、このような担体固定化微生物群は、必要に応じて、布やネット等の通水性や通気性のよい容器に収納して使用してもよい。
【0014】
上記担体としては、これらの他、リボン状やシート状の織布や不織布を用いることができる。
【0015】
このような担体に微生物群を担持させるには、担体と本発明の微生物群分散液とを混合した後、乾燥すればよいが、担体で直接微生物群を培養してもよい。
【0016】
担体の微生物群の担持量は、担持させるときの条件によっても異なるが、好ましくは、5個〜200億個/cm3 、好ましくは10個〜100億個/cm3 である。
【0017】
バチルス・セレウス CCC -bは、排水中の有機物を有効に分解しうる。また、バチルス・セレウス CCC
-bを用いることにより、養豚場、公衆便所等に用いて、脱臭効果が確認された。さらに、バチルス・セレウス CCC -bを用いることにより、リン等の金属の回収機能が確認された。
【0018】
なお、上記バチルス・セレウス CCC -bは、マウスによる経口投与試験によりその安全性が確認された。
【0019】
【実施例】
次に、本発明の実施例について説明する。
まず、次のような組成の培地を作成した。
【0020】
培地
ペプトン 5g
肉エキス 5g
NaCl 2.5g
水 500ml(pH7.0)
【0021】
以上の組成の培地を、1000ml容器に入れ、これにバチルス・セレウス CCC -bを接種し、よく撹拌した後、静置して、30℃で1日間培養した。
【0022】
得られた培養液を遠心分離機にかけ、菌体を分離し、これをよく洗浄し、担体に付着させてパウダーとした。担体としては珪藻土を焼いて、粉砕したものを用いた。
【0023】
このパウダー状のバチルス・セレウス CCC -bを、計量対象へ接種してのCOD、全窒素量および全リン量の経時変化を計量した。試験方法は、CODはJIS0102−17、全窒素量はJIS0102−45.2、そして全リン量はJIS0102−46.2に従っておこなった。計量対象としては、海水での牛乳100倍液に、1リットル当たり0.2gの澱粉を加えたものを用いた。そのままのものを比較例とし、これにバチルス・セレウス CCC -bを接種したものを実施例とした。試験は、20℃に保って行なった。また、各量の測定にあたっては、計量対象液を2時間静置し、その上澄み液を試験液とした。
【0024】
試験の結果を表1に示した。なお、試験は、それぞれサンプルを2つずつ作製し、その平均を測定値とした。
【0025】
【表1】
【0026】
表1分かるように、バチルス・セレウスCCC -bは、水の浄化機能を有し、リンを除去する作用があることが判明した。さらに、1本のままの腐った鰯にバチルス・セレウスCCC
-bのパウダーを振りかけ2時間経過後にその臭いをかいだところ、明らかに臭気が減少していた。
【0027】
このように、バチルス・セレウスCCC -bは、上記の実験で確認された有機物分解機能、リン除去機能に加えて脱臭作用も備えている。[0001]
[Industrial application fields]
The present invention relates to a wastewater treatment method, and more particularly to a wastewater treatment method using microorganisms.
[0002]
[Prior art]
Various bacteria of the genus Bacillus are known as drainage purification bacteria. However, Bacillus microorganisms that have been used for wastewater treatment so far are known to remove and deodorize organic matter in wastewater, but at the same time, no reports have been made on microorganisms that can also remove phosphorus components. There is no.
[0003]
[Problems to be solved by the invention]
Therefore, an object of the present invention is to provide a wastewater treatment method using microorganisms that can remove phosphorus and remove phosphorus and also remove phosphorus .
[0004]
[Means for Solving the Problems]
The inventors of the present invention have eagerly searched for microorganisms that can satisfy all of the above-mentioned elements. As a result, Bacillus cereus CCC-b (deposit number FERM P-16176) It was found that all removal, deodorization and phosphorus removal can be performed.
[0005]
The present invention is based on this finding, and is characterized in that organic matter in the wastewater is removed, deodorized, and phosphorus is removed by bringing Bacillus cereus CCC cb into contact with the wastewater.
[0006]
The Bacillus cereus CCC -b is, Bacillus cereus CCC - b has been deposited with the National Institute of Advanced Industrial Science and Technology Patent Organism Depositary Center as the (accession number FERM P -16176).
[0007]
Hereinafter, this Bacillus
Explain the mycological properties of cereus CCC - b .
[0008]
[0009]
This Bacillus cereus CCC-b showed the atypical property of not degrading starch. Moreover, this Bacillus cereus CCC-b grows well in any medium as long as it is a general nutrient medium.
[0010]
The culture preferably grows well at about 15 to 45 ° C.
[0011]
Bacillus cereus can be dispersed in water or a liquid medium and used for various purposes in the form of a dispersion. Preferably, the microorganism group is immobilized on a carrier and used as a carrier-immobilized microorganism group that facilitates handling.
[0012]
As a carrier for immobilizing a microorganism group, it is preferable that the retention capacity of the microorganism is large and the activation of the microorganism is easy.
[0013]
As the carrier, rocks (for example, pearlite, diatomaceous earth) or a pulverized product thereof, gravel, sand, plastics, ceramics (for example, alumina, silica, natural zeolite, synthetic zeolite), talc and the like are also preferable. A porous material having continuous ventilation holes such as porous plastics is preferred. For example, a fine powder (trade name: pearlite) obtained by holding a pearlite pulverized product at a high temperature and high pressure and heat-treating and then rapidly reducing the pressure to make it porous is preferable because it has the property of causing fungi to grow. In such a porous material, the diameter of the vent is preferably about 2 to 10 μm. The carrier may have any properties such as lump, granule, powder, fine powder or crumb, needle, etc., but preferably has an average particle size of 2 mm or less, particularly 50 μm to 1 mm. Good granular material. Such a carrier-immobilized microorganism group may be housed and used in a container having good water permeability and air permeability such as a cloth or a net as necessary.
[0014]
In addition to these, a ribbon-like or sheet-like woven fabric or non-woven fabric can be used as the carrier.
[0015]
In order to support the microorganism group on such a carrier, the carrier and the microorganism group dispersion of the present invention are mixed and then dried, but the microorganism group may be directly cultured on the carrier.
[0016]
The loading amount of the microorganism group on the carrier varies depending on the loading condition, but is preferably 5 to 20 billion / cm 3 , preferably 10 to 10 billion / cm 3 .
[0017]
Bacillus cereus CCC-b can effectively decompose organic matter in waste water. Also, Bacillus cereus CCC
By using -b , the deodorizing effect was confirmed in pig farms and public toilets. Furthermore, the recovery function of metals such as phosphorus was confirmed by using Bacillus cereus CCC-b .
[0018]
The safety of Bacillus cereus CCC-b was confirmed by an oral administration test using mice.
[0019]
【Example】
Next, examples of the present invention will be described.
First, a medium having the following composition was prepared.
[0020]
Medium Peptone 5g
5g meat extract
NaCl 2.5g
500 ml of water (pH 7.0)
[0021]
The medium having the above composition was placed in a 1000 ml container, inoculated with Bacillus cereus CCC-b , stirred well, allowed to stand, and cultured at 30 ° C. for 1 day.
[0022]
Multiplying the obtained culture to a centrifuge, the cells were separated, which was washed well and the powder is adhered to the carrier. The carrier baked diatomaceous earth, was used as the grinding.
[0023]
This powdery Bacillus cereus CCC-b was inoculated into the object to be weighed, and the changes over time of COD, total nitrogen amount and total phosphorus amount were measured. The test method was carried out according to JIS0102-17 for COD, JIS0102-45.2 for total nitrogen, and JIS0102-46.2 for total phosphorus. As an object to be measured, a solution obtained by adding 0.2 g of starch per liter to 100 times of milk in seawater was used. The one as it was was used as a comparative example, and the one inoculated with Bacillus cereus CCC-b was used as an example. The test was carried out at 20 ° C. Moreover, in measuring each amount, the liquid to be measured was allowed to stand for 2 hours, and the supernatant was used as a test liquid.
[0024]
The test results are shown in Table 1. In the test, two samples were prepared for each, and the average was taken as the measured value.
[0025]
[Table 1]
[0026]
As can be seen from Table 1, Bacillus cereus CCC-b has a water purification function and has been found to have an action of removing phosphorus . In addition, Bacillus Cereus CCC on a single rotten persimmon
When sprinkled with -b powder and smelled after 2 hours, the odor was clearly reduced.
[0027]
As described above, Bacillus cereus CCC-b has a deodorizing action in addition to the organic substance decomposing function and the phosphorus removing function confirmed in the above experiment.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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JP12015897A JP3650687B2 (en) | 1997-04-23 | 1997-04-23 | Wastewater treatment method |
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Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12015897A JP3650687B2 (en) | 1997-04-23 | 1997-04-23 | Wastewater treatment method |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2002017851A Division JP3748822B2 (en) | 2002-01-28 | 2002-01-28 | Novel microorganism belonging to Bacillus cereus species and deodorizing method using the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH10296295A JPH10296295A (en) | 1998-11-10 |
JP3650687B2 true JP3650687B2 (en) | 2005-05-25 |
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ID=14779399
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Application Number | Title | Priority Date | Filing Date |
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JP12015897A Expired - Lifetime JP3650687B2 (en) | 1997-04-23 | 1997-04-23 | Wastewater treatment method |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100459852B1 (en) * | 2002-02-02 | 2004-12-04 | 최진영 | The microbial seeds for the sewage/wastewater treatment and the method for it's development |
KR100512790B1 (en) * | 2003-07-31 | 2005-09-06 | 한국생산기술연구원 | A Mixed Strains for Wastewater Treatment, and A Method for Biological Treatment of Textile Wastewater |
CN117736938B (en) * | 2024-02-07 | 2024-05-17 | 南京万瑞环境科技有限公司 | Microorganism composite flora and application thereof |
-
1997
- 1997-04-23 JP JP12015897A patent/JP3650687B2/en not_active Expired - Lifetime
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JPH10296295A (en) | 1998-11-10 |
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