JP3452347B2 - Water purification agent and water purification method - Google Patents

Abstract

An effective water purifier containing microorganisms other than Bacillus strains and a method of water purification, the water purifier containing Lactobacillus paracasei subspecies paracasei, Enterococcus malodoratus and Candida lipolytica.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a water purification agent and a water purification method, and more particularly to a water purification agent and a water purification method using microorganisms.

[0002]

2. Description of the Related Art Water,
In particular, as a purifying bacterium for purifying waste water, various Bacillus microorganisms are known, but the present invention aims to provide an effective water purifying agent and a water purifying method using microorganisms other than Bacillus. To do.

[0003]

[Means for Solving the Problems] The inventors of the present invention have conducted an intensive search for a microorganism capable of achieving the above-mentioned object, and found that Lactobacillus paracasei subs
p.paracasei), Enterococcus malodoratus (Ent
erococcus malodoratus) and Candida lipoly
When I combined Tika (Candida lipolytica),
We have found that water can be effectively and efficiently purified.

The present invention is based on this finding,
The water purifying agent of the present invention is Lactobacillus paracasei Lactobacillus paracasei.
i subsp.paracasei), Enterococcus malodoratus and Candida
Contains lipandica (Candida lipolytica).

The method for purifying water according to the present invention is characterized in that Lactobacillus paracasei subsp.
racasei), Enterococcus malodoratus (Enteroc
occus malodoratus) and Kyanjida lipolytica (as Candida lipolytica) was present at the same time, it is characterized in that purifying water.

[0006]

In the present invention, by the interaction of the above bacteria and yeasts other than the genus Bacillus, and the interaction between the above bacteria and yeasts and microorganisms existing in nature,
Water can be purified effectively and efficiently.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The specific constitution of the present invention will be described in detail below. The water purifying agent of the present invention is Lactobacillus paracasei Subspecies Paracasei (Lact
obacillus paracasei subsp.paracasei), Enterococcus Marodoratasu (Enterococcus malodoratus) and Kyanjida lipolytica (Candida lipolytic
Contains a).

[0008] Lactobacillus is a Gram-positive bacillus that mainly fermentes sugar to produce lactic acid. Lactobacillus paracasei subspecies paracasei is known to be isolated from dairy products, sewage, silage, clinical materials and the like. .

As the Lactobacillus paracasei subspecies paracasei to be used, the present applicant has proposed DD
As D-a (deposit number: FERM BP-6463 )
National Institute of Advanced Industrial Science and Technology, Patent Microorganism Depositary Center
It is preferable to use those deposited with the over.

Hereinafter, this Lactobacillus paracasei
The mycological properties of DDD-a, which is a subspecies paracasei, will be described.

Test item Test result Morphology Bacillus Gram stainability + spores-motility-attitude toward oxygen facultative anaerobic catalase-produced lactate L (+) gas production from glucose-gas production from gluconate + at 15 ° C Growth + Growth at 45 ° C-Fermentation of sugar Amygdalin + Arabinose-Esculin + Fructose + Galactose + Glucose + Gluconate + Lactose + Maltose + Mannitol + Mannose + Meletitose + Rhamnose + Ribose + Ribose-Rhamnose-Ribnose-Ribnose-Rhamnose-Ribnose- Clucose + trehalose + xylose-GC content of intracellular DNA (mol%) ^{* 1} 46 * ^{1 According to} HPLC method.

[0012] Enterococcus is known as enterococcus, and Enterococcus malodoratus is known to be isolated from cheese.

As the Enterococcus malodoratus to be used, the present applicant has proposed DDD-b (deposit number: FERM B
National Institute of Advanced Industrial Science and Technology as P-6464 )
It is preferable to use the one deposited at the Patent Microorganism Depositary Center .

The mycological properties of DDD-b, which is Enterococcus malodoratus, will be described below.

Test item Test result Morphology Streptococcus gram stainability + spores-motility-attitude toward oxygen facultative anaerobic catalase-gas production from glucose-lactic acid L (+) growth at 15 ° C + 45 ° C Growth in the presence of 6.5% NaCl + growth at pH 9.6 + 40% growth in the presence of bile + hemolytic α-hemolytic arginine dihydrolase-hippuric acid-hydrolysis of esculin + 0.1% methylene blue milk growth -VP reaction-Acid formation xylose-Rhamnose + sucrose + lactose + melibiose + raffinose + meletitose + ^{* 1} glycerol-adonitol- ^{* 2} sorbitol + mannitol + L-arabinose-yellow pigment formation-GC content of intracellular DNA ( mol%) ^{* 2} 40 * 1 Atypical properties * 2 By HPLC method.

The genus Candida is a yeast belonging to imperfect fungi, and since Candida lipolytica has a lipase, it is isolated as a causative bacterium of spoilage such as butter and margarine. There is a separation example.

[0017] As used Kyanjida lipolytica, the applicant, DDD-c (accession number: FERM BP-64
65 ) as an incorporated administrative agency AIST
It is preferable to use the one deposited at the deposit center .

The mycological properties of this Candida lipolytica , DDD-c, will be described below.

Test item Test result Vegetative cell morphology Oval-oval-cylindrical growth type Multipolar sprouting liquid culture Precipitation and film formation are observed (25 ° C, 3 days) Pseudohyphae are formed (25 ° C, 3 days) ) Fungal filaments are formed (25 ° C., 3 days) ^* Ascospore Fermentable glucose-galactose-sucrose-maltose-lactose with no formation in Adams, Gorodkova, malt, YM, V-8 and potato dextrose media. -Raffinose-Assimilating galactose-Sucrose-Maltose-Cellobiose-Trehalose-Lactose-Melibiose-Raffinose-Meletitose-Starch-D-xylose-L-arabinose-D-ribose + L-rhamnose-Glycerol + Erythritol D + Ribitol. − N'nitoru + lactate + succinate + Citrate + Inositol - nitrate assimilation - 37 Growth at ° C. - Growth in vitamin deficiency medium - color degradation weak DBB urea - Lipase +

The above-mentioned three kinds of bacteria grow well in any medium as long as it is a general nutrient medium, but Lactobacillus paracasei subspecies paracasei and Enterococcus malodoratus are particularly preferably MRS medium, and Candida lipolytica is preferable. ,
Particularly, YM medium is preferable.

The culture of any of the bacteria is preferably 15 to
It grows well at about 45 ° C.

In the water purifying agent of the present invention, it is preferable that the above-mentioned three kinds of bacteria are preferably a carrier-immobilized microorganism group in which a microorganism group is immobilized on a carrier to facilitate handling.

The above 3 contained in the carrier-immobilized microorganism group
The amount ratio of each of the species of bacteria is not particularly limited. That is, when the above-mentioned various bacteria are allowed to coexist in water, the bacteria are stabilized at a substantially constant rate depending on storage or culture conditions.
However, if the storage or culturing conditions are appropriately set so that the quantitative ratios shown below are obtained, the water purification function can be exhibited extremely well.

Lactobacillus paracasei Subspecies paracasei: 20-60% Enterococcus malodoratus: 20-60% Candida lipolytica : 10-30%

As the carrier for fixing the microbial group, it is preferable that the microbial holding capacity is large and the microbial activation is easy.

As the carrier, rock (for example, pearlite, diatomaceous earth) or a crushed product thereof, gravel, sand, plastics,
Ceramics (eg, alumina, silica, natural zeolite, synthetic zeolite), talc and the like are also preferable, and particularly,
Porous materials having continuous vents such as porous ceramics and porous plastics are preferred. For example, fine powder (trade name: pearlite) made by crushing pearlite crushed product at high temperature and high pressure, heat-treating, and then rapidly depressurizing it
It is suitable because it has the property of growing fungi. In such a porous material, the diameter of the ventilation hole is 2 to 10 μm.
It is preferably about the same. The carrier may be in the form of lumps, granules, powders, fine powders or lumps, needles, etc., but preferably has an average particle size of 2 mm or less, particularly 50 μm to 1 mm. A granular material of a certain degree is good. If necessary, such carrier-immobilized microorganisms may be stored in a container having good water permeability or air permeability such as cloth or net before use.

In addition to these, a ribbon-shaped or sheet-shaped woven or non-woven fabric can be used as the carrier.

In order to support the microorganism group on such a carrier, the carrier and the dispersion liquid in which the microorganism group of the present invention is dispersed may be mixed and dried, but the microorganism group may be directly cultured on the carrier. Good.

The amount of the microbial group carried on the carrier varies depending on the conditions under which it is carried, but is preferably 5 to 20.
0.0 billion / cm ³ , preferably 10 to 10 billion / cm
^{Is 3} .

The above-mentioned three kinds of bacteria can effectively decompose organic substances in wastewater by interaction.

The safety of each of the above three types of bacteria was confirmed by an oral administration test in mice.

The water purification process of the present invention, in the water to be treated, Lactobacillus para casei subsp. Paracasei, Enterococcus Marodoratasu and key
The presence of Gandhi lipolytica at the same time purifies the water. That is, the water purification agent of the present invention is brought into contact with water to be treated. As water to be treated, general domestic wastewater, industrial wastewater, water from rivers, swamps, ponds and the like can be preferably mentioned.

When purifying water, the water purifying agent of the present invention is
1 for 1 liter of water to be treated, calculated as the above carrier
It is preferable to use about 50 grams, especially about 5 to 20 grams.

It is desirable to perform air ring during the purification process of water.

The water purification method of the present invention does not require any other conditions.

[0036]

EXAMPLES Next, examples of the present invention will be described. First, a medium having the following composition was prepared. Medium Peptone 5 g Meat extract 5 g NaCl 2.5 g Water 500 ml (pH 7.0)

The culture medium having the above composition was placed in a 1000 ml container, and the bacteria DDD-
a, DDD-b and DDD-c were inoculated, stirred well, allowed to stand and cultured at 30 ° C. for 1 day. The amount ratio of DDD-a, DDD-b, and DDD-c in the culture solution was 40:
It was about 40: 20%.

The obtained culture broth was subjected to a centrifuge to separate the bacterial cells, which were thoroughly washed and adhered to a carrier to obtain a powdery water purifying agent. The carrier used was diatomaceous earth baked and crushed. The amount of microorganisms carried on the carrier was about 5 billion / cm ³ .

10 g of this powdery water purifying agent,
The changes over time in biochemical oxygen demand (BDO), chemical oxygen demand (COD), suspended solids (SS), and transparency were measured after inoculating 20 liters of the subject to be weighed. As for the test method, BDO is JIS K0102-21, JIS
K 0102-32.3 and COD are JIS K010
2-17, SS was carried out in accordance with Announcement No. 59, Appendix 6, and transparency was conducted in accordance with the clean water test method [general properties]. Further, as the object of measurement, the water of the pond in Gyosen Park (Edogawa Ward, Tokyo) was collected and used. The sampling date was July 4, 1998, and 20 liters of each sampled water was put into two containers, and 10 g of the above water purifying agent was put into one of the water and aired for treatment (Example). The water in the other container was left as it was (comparison), and the BDO and the like after 5 days were measured. When measuring each amount, the liquid to be measured was left for 2 hours, and the supernatant was used as a test liquid. The test results are shown in Table 1.

[0040]

[Table 1]

As can be seen from Table 1, the present water purifying agent effectively purifies water.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ identification code FI C12R 1: 225) C12R 1: 225 (58) Fields investigated (Int.Cl. ⁷ , DB name) C02F 3/34 C12N 1 / 20

Claims (2)

(57) [Claims] 1. Lactobacillus paracasei subs
p.paracasei), Enterococcus malodoratus (Ent
erococcus malodoratus) and Candida lipoly
Water purification agent containing Tika (Candida lipolytica). 2. Lactobacillus in the water to be treated
Paracasei Subspecies Paracasei (Lactobac
illus paracasei subsp.paracasei), Enterococcus malodoratus and
Scan di Dali poly Tikka and (Candida lipolytica) was present at the same time, purification method for purifying water.