JP2002316936A - Antibacterial agent and anti-inflammatory agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an antibacterial agent and an anti-inflammatory agent which are characterized by containing one or more extracts selected from Huamansamana (nomenclature: Jacaranda copaia), Hercampuri (nomenclature: Gentiana prostrata, Gentiana nitida or Gentiana alborosea) and Canchalagua (nomenclature: Sisyrinchium vaginatum). SOLUTION: The antibacterial agent and an anti-inflammatory agent are characterized by containing one or more extracts selected from Huamansamana (nomenclature: Jacaranda copaia), Hercampuri (nomenclature: Gentiana prostrata, Gentiana nitida or Gentiana alborosea) and Canchalagua (nomenclature: Sisyrinchium vaginatum). The extracts of Huamansamana (nomenclature: Jacaranda copaia), Hercampuri (nomenclature: Gentiana prostrata, Gentiana nitida or Gentiana alborosea) and Canchalagua (nomenclature: Sisyrinchium vaginatum) have an excellent antibacterial property against Staphylococcus aureus and further have an excellent anti-inflammatory property against skin inflammation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a method for preparing Staphylococcus aureus, Acne,
The present invention relates to an antibacterial agent excellent in antibacterial properties against hemolytic streptococci and the like, and an antiinflammatory agent excellent in skin inflammation due to atopic dermatitis and acne.

[0002]

2. Description of the Related Art Staphylococcus aureus is known to be closely related to various skin diseases. For example, in atopic dermatitis, the skin damaged by scratching
It is thought that this bacteria causes inflammation by secondary contamination. As an antibacterial agent against Staphylococcus aureus, an antibiotic is used in the clinical field, but there are many problems such as expression of resistant bacteria (MRSA). Acne is closely related to acne, and hemolytic streptococci cause infection.

[0003]

In view of such circumstances, an object of the present invention is to provide an antibacterial agent derived from a natural product, which exhibits excellent antibacterial properties against Staphylococcus aureus, acne bacteria, hemolytic streptococci, and the like. .

[0004]

Under these circumstances, the present inventors have conducted intensive studies, and as a result, the extract of Uwamansamana, Ercampuri, and Kansaragua has been found to be superior to Staphylococcus aureus, Acne, Hemolytic streptococci and the like. It has been found that the present invention has excellent antibacterial properties and an excellent effect of improving skin inflammation, and has completed the present invention.

[0005] Huamsamana used in the present invention (Huam
ansamana (scientific name: Jacaranda copaia) is a tall tree of the family Noctuidae. It is mainly distributed in Central America and northern South America and is widely used as a crude drug. Other names for Uwamansamana include Calova de Matt and Kobaja.

[0006] Hercampuri (Hercam) used in the present invention
puri, scientific name: Gentiana prostrata, Gentiana nitida
Or Gentiana alborosea) is an annual of the Gentian family. It is widely distributed mainly in tropical America and is widely used as a crude drug.

[0007] Cancaragua (Canc) used in the present invention
halagua (scientific name: Sisyrinchium vaginatum) is a perennial plant of the Iridaceae family. It is widely distributed mainly in South America and is widely used as a crude drug.

[0008] The extract of Uwamansamana used in the present invention is extracted from a part or the whole of a plant such as leaves, stems, flowers, fruits, seeds, roots, bark, and xylem of Uwamansana. Preferably, those obtained by extracting from leaves and stems are good.

The Hercampuri extract used in the present invention is extracted from a part or the whole of a plant such as Hercampuri leaves, stems, flowers, fruits, seeds and roots. Preferably, those obtained by extracting from the whole plant above the ground are good.

The extract of Kansaragua used in the present invention is extracted from a part or the whole of a plant such as leaves, stems, flowers, fruits, seeds and roots of Kansaragua. Preferably, those obtained by extracting from the whole plant above the ground are good.

[0011] The extraction method is not particularly limited, and may be, for example, a heat-extracted one or a room-temperature-extracted one.

Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.) and liquid polyhydric alcohols (glycerin, propylene glycol, 1-butanol). , 3-butylene glycol, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, propyl) Ether, tetrahydrofuran, etc.). Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, propylene glycol and 1,3-butylene glycol. These solvents may be used alone or in combination of two or more.

The extract of Uwamansa mana, Ercampuri and Kansharagua may be used as it is as an extracted solution, or may be used after being subjected to treatment such as concentration, dilution, and filtration as required. Further, the extracted solution may be subjected to a treatment such as concentration and drying, spray drying, and freeze drying to be used as a dried product.

In the antibacterial agent of the present invention, the above extract may be used as it is, as long as the effect of the extract of Uwamansamana, Ercampuri and Kansharagua is not impaired.
Oils and fats, waxes, which are components used in normal antibacterial agents,
Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, ultraviolet absorbers, thickeners, pigments, antioxidants,
Components such as a whitening agent and a chelating agent can be blended.

The antibacterial agent of the present invention can be used in any of cosmetics, quasi-drugs and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels, aerosols, essences, Packs, detergents, bath preparations, foundations, powders, lipsticks, ointments, cataplasms and the like applied to the skin can be mentioned.

[0016]

EXAMPLES In order to explain the present invention in detail, examples of the production of the extract used in the present invention and experimental examples will be given below, but the present invention is not limited thereto.

Production Example 1 Hot water extract of Uwamansamana Purified water (400 g) was added to 20 g of dried leaves and stems of Uwamansamana, extracted at 95-100 ° C for 2 hours, filtered, concentrated, and freeze-dried. As a result, 5.0 g of hot water extract of Uwamansa mana was obtained.

Production Example 2 50% ethanol extract of awamansamana 1.0 kg of purified water and 1.0 kg of ethanol were added to 100 g of dried leaves of awamansamana, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness. Thus, 44 g of ethanol extract of Uwamansamana was obtained.

Production Example 3 Hot water extract of Hercampuri Erycampuri whole plant dry matter of 20g, purified water 400g was added, 9
After extraction at 5-100 ° C for 2 hours, the mixture was filtered, and the filtrate was concentrated and lyophilized to obtain 5.2 g of a hot water extract of Ercampuri.

Production Example 4 50% Ethanol Extract of Hercampli A 1.0 kg of purified water and 1.0 kg of ethanol were added to 100 g of dried leaves and stems of Hercampuri, and extracted at room temperature for 7 days.
After filtration, the filtrate was concentrated to dryness to obtain 23 g of an ethanol extract of Ercampli.

Production Example 5 Hot Water Extract of Kansaragua A 400 g of purified water was added to 20 g of dried whole plant of Kansaragua, extracted at 95-100 ° C for 2 hours, filtered, concentrated, and freeze-dried. Hot water extract of Kansaragua
3.0 g was obtained.

Production Example 6 50% ethanol extract of Kansaragua A 100 kg of dried leaves and stems of Kansaragua are filled with 1.0 kg of purified water.
Then, 1.0 kg of ethanol and 1.0 kg of ethanol were added, and the mixture was extracted at normal temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 12 g of an ethanol extract of Kansaragua.

Next, experimental examples will be described in order to explain the effects of the present invention in detail.

Experimental Example 1 Antibacterial activity test (Method) Staphylococcus aur
Using the eus ATCC 6538, the in vitro antibacterial activity of the following extracts was measured according to the standard plate dilution method of the Japanese Society of Chemotherapy.

(1) Hot water extract of Uwamansamana (Production Example 1) (2) 50% ethanol extract of Uwamansamana (Production Example 2) (3) Hot water extract of Ercampuri (Production Example 3) (4) Ercampuri 50% Ethanol Extract of Kansaragua (5) Hot Water Extract of Kansaragua (Production 5) (6) 50% Ethanol Extract of Kansaragua (Production 6)

In the agar plate dilution method, each extract is added to a medium at a concentration of 0.5, 0.25, 0.125 and 0.0625%, and a minimum growth inhibitory concentration (hereinafter referred to as MIC, unit:
%). In addition, a soybean casein digest agar medium (manufactured by Eiken Chemical Co., Ltd.) was used as a medium, and the cells were cultured at 37 ° C. for 3 days.

(Results) The results are shown in Table 1.

[0028]

[Table 1]

From the above Table 1, it is clear that the extract of the present invention has excellent antibacterial properties against Staphylococcus aureus.

EXPERIMENTAL EXAMPLE 2 Use Test Using 1.0% aqueous solutions of Production Examples 1, 3 and 5, 10 adults (5 males) having atopic dermatitis-like inflammation on their skin
And five women) for one month.
After use, it was determined by a questionnaire survey about the improvement of skin inflammation. As a result, the anti-inflammatory agent characterized by containing extracts of Uwamansamana, Hercampuri and Kansharagua showed excellent skin inflammation improving effect (Table 2). There was no skin trouble during the test, and there was no problem in safety.

[0031]

[Table 2]

[0032]

As described above, the extracts of Uwamansa mana, Hercampuri and Kansharagua of the present invention have excellent antibacterial properties against Staphylococcus aureus and the like. In addition, it exhibited an excellent improvement effect on atopic dermatitis-like skin inflammation.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Hajio Kojima 2-7 Tomicho, Nishi-ku, Nagoya City Japan Menard Cosmetics Co., Ltd. (72) Inventor Kazutoshi Osumi 2-7 Tomicho, Nishi-ku Nagoya City Japan Menard Cosmetics Co., Ltd. (72) Inventor Tomonori Katata 2-7 Torimicho, Nishi-ku, Nagoya City Menard Co., Ltd. F-term (reference) 4C088 AB12 AB67 AB72 AC03 AC04 AC05 AC06 BA09 BA10 CA05 CA06 CA11 MA07 ZA89 ZA90 ZB11 ZB13

Claims (4)

[Claims] 1. An antibacterial agent comprising one or more extracts selected from Uwamansamana, Hercampuri and Kansharagua. 2. The antibacterial agent according to claim 1, which is effective against Staphylococcus aureus. 3. The antimicrobial agent according to claim 1, wherein the antimicrobial agent is an extract with one or more solvents selected from water, lower alcohols, and liquid polyhydric alcohols. 4. An anti-inflammatory agent comprising one or more extracts selected from Uwamansamana, Ercampuri and Kansharagua.