JP2002275043A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin care preparation that includes active ingredients originating from plants and has excellent actions of increasing skin elasticity as well as of whitening the skin. SOLUTION: The objective preparation characteristically includes an extract from leaves of a plant in Platanaceae.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin. More specifically, the present invention relates to an external preparation for skin containing a plant-derived active ingredient and having excellent skin elasticity-enhancing action and whitening action.

[0002]

2. Description of the Related Art Collagen is a fibrous protein that forms connective tissues such as human skin, tendons and bones. Decrease in skin collagen with aging contributes to skin deterioration, and collagen is an important raw material to be mixed with various active ingredients in skin external preparations. As raw materials for collagen, bones and skins of cattle and pigs are used. Regarding animal raw materials, not a few people feel uneasy about infection with animal-derived diseases in recent years. In practice, it is unlikely that a skin external preparation will cause infection, but in the case of cosmetics and other products that appeal to sensitivity, one of the options is to meet the needs of users. In the case of animal-derived materials,
There are some problems in their securing and handling. A plant belonging to the genus Suzukenoki, which is known as Platanus, is often used as a street tree. As plants of the genus Suzume, sycamore (scientific name: Platanus orientalis or Platanus vulgaris), American sycamore (scientific name: Platanus occidentalis), maple bass sycamore (scientific name: Platanus x acerifolia, Platanus x hispan)
ica or Platanus x hybrida), a variant of maple bass perennial (Platanus occidentalis x Platanus)
orientalis or Platanus orientalis var.acerifol
ia) are known. American sycamore is a deciduous tree that is 10 to 30 meters in height, and rarely 50 meters in height.
Leaf pieces are close to triangles. Native to North America, but also distributed in Japan. With respect to the usefulness of plants of the genus Suzuka, Japanese Patent Application Laid-Open No. Hei 8-317739 proposes a maple leaf moss with increased guard cells and an improved ability to absorb air pollutants. The usefulness of the plants of the genus Periwinkle as street trees, furniture, and utensils has been reported, and a mass propagation method by tissue culture has been proposed. However, there are no reports or proposals on the use of plants of the genus Suzume in cosmetics, pharmaceuticals, and the like.

[0003]

DISCLOSURE OF THE INVENTION The object of the present invention is to provide an external preparation for skin containing a plant-derived active ingredient and having excellent skin elasticity and whitening effects.

[0004]

The present inventors have conducted intensive studies to solve the above-mentioned problems. As a result, an external preparation for skin containing an extract of a leaf of a plant belonging to the genus Suzumecaceae is excellent. It has been found that it has a skin elasticity enhancing effect and a whitening effect, and based on this finding, the present invention has been completed. That is, the present invention provides (1) an external preparation for skin characterized by containing an extract of the leaves of a plant belonging to the genus Suzumecaceae, (2) an extract from which a dissolved portion is extracted in the presence of cellulase. 2. The skin external preparation according to item 1, which is an extracted extract, and (3) the skin external preparation according to item 1, wherein the plant belonging to the genus Suzukaenoki is a sycamore.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The skin preparation for external use of the present invention contains an extract of a leaf of a plant belonging to the genus Suzukaenoki of the family Suzukake.
The extract of the leaves of the plant of the genus Suzukake, which is contained in the external preparation for skin of the present invention, is composed of constituent amino acids similar to collagen, has an excellent whitening effect, is not allergic and does not have irritation. . The external preparation for skin of the present invention prevents wrinkles, improves skin firmness, exhibits an excellent whitening effect, and has a good feeling on use on the skin. There are no particular restrictions on the plants of the genus Suzukaenoki used in the present invention, and examples thereof include Suzukakenoki, U.S. sycamore, Momijibasuzumoke and its variants.
Among these, American sycamore can be particularly preferably used. There is no particular limitation on the leaf extract of the plant belonging to the genus Suzukake, which is used in the present invention, but an extract from which the dissolved portion is extracted in the presence of cellulase can be particularly preferably used. Cellulase has the enzyme number EC
3.2.1.4. Is an enzyme that hydrolyzes cellulose,
It is an enzyme that mainly produces cellobiose and is present in bacteria, filamentous fungi, wood rot fungi, digestive juice of mai-mai, plant tissues, and the like, but commercially available cellulase can also be used. In many cases, microorganisms secrete enzymes that are induced by the presence of cellulose and have several different properties. Cellulase activity was measured using cellulose powder, carboxymethylcellulose, and cellooligosaccharides as substrates, reducing turbidity,
It can be measured by a decrease in viscosity and an increase in reducing power. The optimum pH for cellulase degradation activity is said to be in the range of 3-5.

There is no particular limitation on the method for obtaining a leaf extract of a plant belonging to the genus Suzukake, which is used in the present invention.
For example, a cellulase is allowed to act on a dried leaf of a plant belonging to the genus Suzumecaceae, and the portion soluble in water can be collected. The action time of the cellulase, considering the concentration of the cellulase, the degree of leaf shredding, the action temperature, etc.
It can be selected as appropriate. It is preferable that the extract of the leaves of the plant of the genus Suzukake, which is used in the present invention, is a purified product. There is no particular limitation on the method of obtaining a purified product of the extract, but it is preferable to purify from the leaf stage of the plant before extraction, rather than purifying after extraction. The method for purifying plant leaves is not particularly limited. For example, plant leaves are preferably extracted using an aqueous solution of a water-soluble organic solvent in an amount of preferably 40% by weight or more, more preferably 55% by weight or more to remove a soluble portion. Then, insolubles can be collected by a method such as centrifugation, filtration, or decantation, and the process can proceed to the next step. The water-soluble organic solvent used is not particularly limited, and examples thereof include methanol, ethanol, and acetone. Alternatively, the leaves of the plant may be extracted with water to remove the soluble portion, and the insolubles may be collected by a method such as centrifugation, filtration, decantation, etc., and then proceed to the next step. One of these purification methods can be used alone, or two or more can be used in combination in any order. Further, the leaves thus purified are immersed in water, and a portion soluble in water in the presence of cellulase is extracted and collected, and then insoluble substances are removed by a method such as centrifugation, filtration, and decantation. Can be. The concentration of the obtained extract is appropriately adjusted by dilution, concentration under reduced pressure, freeze-drying and the like, and can be used as a raw material for external preparation for skin.

[0007] The external preparation for skin of the present invention can appropriately contain necessary components depending on its use and dosage form. Such ingredients include, for example, animal and vegetable oils and fats such as lanolin, mink oil, horse oil, almond oil, castor oil, jojoba oil, Medford oil, olive oil, cholesterol, lanolin alcohol, phytosterols and other sterols derived from animals and plants. And their derivatives, solid paraffin,
Minerals such as ceresin, whale wax, beeswax and carnauba wax, waxes derived from animals and plants, hydrocarbon oils such as liquid paraffin and squalane, higher alcohols such as lauryl alcohol, cetanol, cetostearyl alcohol, oleyl alcohol, lauric acid, stearic acid , Higher fatty acids such as oleic acid, polyoxyethylene oxypropylene glycol, isopropyl myristate,
Synthetic oils such as isopropyl palmitate, cetostearyl isooctanoate and alkyl isostearates, polyoxyethylene alkyl ether sulfates,
Surfactants such as alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, N-coconut fatty acid acyl-L-glutamate, polyoxyethylene higher alkyl ether, polyoxyethylene higher fatty acid ester, poly Surfactants such as oxyethylene hydrogenated castor oil, polyhydric alcohols such as glycerin, sorbitol, propylene glycol and 1,3-butylene glycol, lower alcohols such as ethanol and isopropyl alcohol, hyaluronic acid salts and pyrrolidone carboxylic acid salts , Humectants such as hydrolyzed collagen solution,
Cationic rinse agents such as cationized dextran, carrageenan, xanthan gum, polyvinyl alcohol,
Thickeners such as carboxyvinyl polymers, plant extracts such as arnica extract, aloe extract, seaweed extract, chamomile extract, licorice extract, kina extract, garlic extract, melissa extract, ultraviolet absorbers, antioxidants, chelating agents, Perfumes, pigments, antibacterial agents and the like can be mentioned. The dosage form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include lotions, creams, gels, emulsions, packs, and ointments.

[0008]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, which should not be construed as limiting the present invention. Example 1 (Skin external preparation raw material) 100 kg of dried leaves of American sycamore was cut into small pieces, and
After adding 100 L of a weight% ethanol aqueous solution and stirring, insoluble materials were collected using a filter cloth. After 100 L of purified water was added to the collected insolubles and stirred, the insolubles were collected using a filter cloth. Furthermore, purified water 10
The operation of adding 0 L, stirring, and collecting insolubles was repeated four times. Next, 100 L of purified water and 1 L of 10% by weight cellulase aqueous solution were added to the insoluble matter, and the mixture was stirred at 35 ° C. for 4 days, and then centrifuged to separate a supernatant. The separated supernatant was adjusted to pH 4.0 by adding 5 mol / L hydrochloric acid and stirred at 15 ° C. for 4 days. This liquid was centrifuged to remove the precipitate, and the obtained supernatant was dried at 5% by weight.
It concentrated under reduced pressure so that it might become. An aqueous solution of sodium hydroxide was added to the concentrated solution under reduced pressure to adjust the pH to 5.4, and then filtered through a 0.2-micron filter to remove insolubles. Got. The extract was decomposed by adding 6 mol / L hydrochloric acid to the obtained filtrate, and amino acids were analyzed using an amino acid analyzer. The results are 11.1% by weight of hydroxyproline, 11.7% by weight of glycine, and 8.6% by weight of proline. Three kinds of amino acids which are specifically high in collagen are also contained in a lot of extracts of leaves of the American sycamore. This extract was determined to be a substance similar to vegetable collagen. Example 2 (Evaluation of Effectiveness) The filtrate obtained in Example 1 was freeze-dried, and an extract of the leaf of Suzumekanoki was isolated. A normal human melanocyte [Kurabo Co., Ltd., Melanopack] was added with a leaf extract of American sycamore to a concentration of 0.05% by weight or 0.10% by weight, and together with a control sample to which no extract was added, The cells were subcultured for 6 days according to a conventional method, and the effect of melanocytes on melanin production was examined by visually judging the color tone of the cell pellet. Extract with concentration 0.05
The sample added to be wt% was clearly whiter than the control sample. 0.10% by weight extract
The sample added so as to be almost completely whitened. In addition, the number of cells was hardly decreased as compared with the control sample. Example 3 (Evaluation of safety) The filtrate containing the extract of the leaves of the American sycamore leaf obtained in Example 1 was subjected to an allergy test, a primary skin irritation test and a cumulative irritation test using guinea pigs. went. No allergy or irritation was observed.

Example 4 (Preparation of skin external preparation) 15.0 parts by weight of squalane, 5.0 parts by weight of cetyl 2-ethylhexanoate, 5.0 parts by weight of olive oil, 3.0 parts by weight of stearic acid, stearyl alcohol 3 2.0 parts by weight, beeswax 3.0 parts by weight, polyoxyethylene (20 mol) oxypropylene (4 mol) cetyl ether 1.0 part by weight, polyoxyethylene (50 mol) hydrogenated castor oil 1.0 part by weight, A mixture of 1.0 part by weight of emulsified glyceryl monostearate, 0.2 part by weight of methylpolysiloxane (300 cSt) and 0.2 part by weight of methyl parahydroxybenzoate was added.
The components were prepared. 5.0 parts by weight of a filtrate containing 10.0 parts by weight of glycerin, 5.0 parts by weight of 1,3-butylene glycol, 5% by weight of the extract of leaves of the sycamore leaf obtained in Example 1, and purified water 42 .6 parts by weight, B
The components were prepared. The A component and the B component were each heated to 70 ° C., and the A component was gradually added to the B component with stirring, and then cooled to 30 ° C. with slow stirring to obtain a skin external preparation cream. Comparative Example 1 (Preparation of external preparation for skin) The same procedure as in Example 4 was carried out except that purified water was used instead of the filtrate containing 5% by weight of the extract of the leaves of the American sycamore leaf obtained in Example 1. Thus, a skin external preparation cream was prepared. Example 5 (Evaluation of skin external preparation) Sensory evaluation of the skin external preparation cream prepared in Example 4 and the skin external preparation cream prepared in Comparative Example 1 was performed using 22 women aged 20 to 64 as panelists. Was. The face was divided into left and right sides, and the cream prepared in Example 4 was applied to one side, and the cream prepared in Comparative Example 1 was applied to the other side twice a day for three months. Three months later, improvement of skin firmness,
The three items of whitening effect and feeling of use on the skin were evaluated on a seven-point scale according to the following evaluation criteria. +++: The cream of Example 4 is much better. ++: The cream of Example 4 is much better. +: The cream of Example 4 is slightly better. ±: There is no difference between the cream of Example 4 and the cream of Comparative Example 1. -: The cream of Comparative Example 1 is slightly better. -: The cream of Comparative Example 1 is considerably better. ---: The cream of Comparative Example 1 is much better. The evaluation results are summarized in Table 1.

[0010]

[Table 1]

As can be seen from Table 1, the number of panelists who claim that the cream for external use of Example 4 is better than the cream of Comparative Example 1 is overwhelming, improving skin firmness, whitening effect and improving skin. The effect of the extract of the leaf of Suzuka moss on the feeling of use of citrus was confirmed.

[0012]

EFFECT OF THE INVENTION A plant of the genus Suzukenoki of the family Suzukake,
In particular, the extract of the leaves of American sycamore has similar constituent amino acids to collagen, has a whitening effect, has no safety problems, and a skin external preparation containing this improves skin firmness and has a whitening effect. There is also a good feeling of use. By extracting collagen-like substances from plants, people with discomfort in animal-derived materials can also be used,
In addition, by using plants as raw materials, it is possible to diversify the raw materials while sufficiently securing the supply stability of the raw materials.

Claims (3)

[Claims] 1. An external preparation for skin, comprising an extract of a leaf of a plant belonging to the genus Suzukake of the family Suzukake. 2. The external preparation for skin according to claim 1, wherein the extract is an extract obtained by extracting a dissolved portion in the presence of cellulase. 3. A plant belonging to the genus Suzukenoki of the family Suzukake,
2. The external preparation for skin according to claim 1, which is a Japanese sycamore.