JP2002191387A - Clear supernatant culture liquid of lactobacillus, method for manufacturing the same, and skin care preparation using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a clear supernatant culture liquid for lactobacillus and a method for manufacturing the same reduced in content of a flavor component of the liquid and a skin care preparation using the same. SOLUTION: The clear supernatant culture liquid of lactobacillus and a method for manufacturing the same can reduce the amount of diacetyl which is understood as a flavor component having a smell of fermentation by excluding oxygen in the culture medium of the lactobacillus in a medium using milk as principal ingredient, and a composition of a favorite skin care preparation using the clear supernatant liquid is prepared.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for producing a culture supernatant of a lactic acid bacterium in which a specific odor component is reduced, and more particularly, to a method for reducing a odor component which is perceived as a characteristic fermentation odor generated in a fermentation process of a lactic acid bacterium. The present invention relates to a lactic acid bacteria culture supernatant, a method for producing the same, and a highly palatable skin external preparation composition obtained by blending the same.

[0002]

2. Description of the Related Art The culture supernatant of lactic acid bacteria consists of whey of a culture obtained by inoculating a lactic acid bacterium into a medium containing milk as a main component.
Moisturizing effect as an external preparation for skin (Journal of the Japan Cosmetic Science Society, 6 (4),
p238,1982), antioxidant effect (Abstract of the 7th Annual Meeting of the Japanese Cosmetic Society, 59,1982), photoprotection (8th Annual Meeting of the Japanese Cosmetic Society, 210,1983), skin flora Numerous effects have been reported, such as a control effect of lipase and a skin pH control effect (Summary of the 9th Annual Meeting of the Japanese Society of Cosmetic Science, 132, 1984).

[0003] However, the culture supernatant of lactic acid bacteria contains a trace amount of aroma components produced by lactic acid fermentation. Therefore, when the culture supernatant of lactic acid bacteria is added to cosmetics or the like, the peculiar fermentation smell is associated with deterioration of the quality such as spoilage of the product, and as a result, there is a problem that the palatability is reduced.

[0004] In order to solve this problem, methods for removing aroma components from the culture supernatant of lactic acid bacteria have been studied.
A method is known in which whey is heated under reduced pressure to partially evaporate aroma components in the whey until the aroma components in the whey are substantially removed (JP-A-58-192811, etc.). However, this method has a problem that the removal of the fragrance component is incomplete and that the whey is thermally denatured.

[0005]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a lactic acid bacteria culture supernatant in which the flavor components of the lactic acid bacteria culture supernatant are reduced, a method for producing the same, and a skin external preparation composition using the lactic acid bacteria culture supernatant. Is to do.

[0006]

Means for Solving the Problems In order to solve such problems, the present inventors have conducted intensive studies and as a result, when lactic acid bacteria are cultured in a medium containing milk as a main component, oxygen is eliminated by removing oxygen. It has been found that the amount of diacetyl, which is an aroma component felt as a fermentation odor, can be significantly reduced. Furthermore, the present inventors have found that it is possible to further reduce the amount of other aroma components by further treating the lactic acid bacteria culture thus produced with a carbonate, thereby completing the present invention.

That is, the present invention provides a method of inoculating a medium containing milk as a main component with lactic acid bacteria, culturing the medium under replacement of an inert gas, and separating a supernatant from the obtained culture of lactic acid bacteria. An object of the present invention is to provide a lactic acid bacterium culture supernatant obtained with reduced aroma components.

The present invention also relates to a method for producing a lactic acid bacterium culture supernatant, which comprises inoculating a lactic acid bacterium into a medium containing milk as a main component, culturing the lactic acid bacterium, and obtaining a supernatant from the obtained lactic acid bacterium culture. It is intended to provide a method for producing a culture supernatant of a lactic acid bacterium with reduced aroma components, wherein the culture is performed in the presence of an inert gas.

Furthermore, the present invention provides a composition for external use on the skin, characterized by containing the culture supernatant of a lactic acid bacterium having a reduced flavor component.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The culture supernatant of the present invention with reduced aroma components (hereinafter sometimes referred to as "odorless culture supernatant")
Can be obtained by culturing lactic acid bacteria after inoculating the medium with lactic acid bacteria under replacement of an inert gas. The inert gas used in the production of the odorless culture supernatant of the present invention includes reactive gases such as nitrogen gas, argon gas, carbon dioxide gas, helium gas, neon gas, krypton gas, xenon gas, radon gas, hydrogen gas, and propane gas. And nitrogen gas, argon gas, carbon dioxide gas, and helium gas are particularly preferable in terms of cost, safety, and the like.

The inert gas may be in an amount sufficient to substantially deoxygenate the lactic acid bacteria fermentation medium. For example, when nitrogen gas is used, the cooling step after the lactic acid bacteria culture medium is sterilized at a high temperature. In step 2, a sufficient amount of nitrogen gas is injected to compensate for the reduced pressure due to the liquefaction of water vapor, and the state of positive pressure is constantly maintained. In addition, it is preferable to keep in an anaerobic state by continuously injecting nitrogen gas or by closing during culture.

The production of the odorless culture supernatant of the present invention can be carried out according to a conventional method for culturing lactic acid bacteria, except for the above. That is, lactic acid bacteria used for lactic acid fermentation can be used for lactic acid bacteria culture without any particular limitation as long as they are commonly used in foods and drinks such as lactic acid bacteria beverages, and examples thereof include Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus genus such as Lactobacillus casei, Lactococcus genus such as Lactococcus lactis,
Streptococcus lactis, Streptococcus
Examples include Streptococcus, such as Thermophilus, Leuconostoc, and Bifidobacterium. Particularly preferred is Streptococcus thermophilus.

The milk used in the culture medium for lactic acid bacteria culture is not particularly limited. For example, animal milk such as human milk, cow milk and goat milk, skim milk, milk powder and skim milk powder of these animal milks can be used. And the like, and any of them can be suitably used, but the ease of processing after lactic acid fermentation, the availability,
From the viewpoint of economy and the like, skim milk or reduced skim milk is preferred.

Further, as long as the influence on the flavor of the product is within an acceptable range or within the range that does not affect the effect of the product of the present invention, yeast extract and peptone which are usually added to a medium for fermenting lactic acid bacteria are used. Growth-promoting substances such as L-
A reducing agent such as ascorbic acid and L-cysteine may be added.

Furthermore, the culture conditions after inoculation of the lactic acid bacteria are not particularly limited, and any conditions generally used for culturing lactic acid bacteria can be suitably used. Specifically, for example, after heating and sterilizing the above-described culture medium at a temperature of about 100 ° C. to 115 ° C. for about 15 to 100 minutes, deoxygenation is performed by sufficiently injecting an inert gas such as nitrogen gas through a filter. do it. afterwards,
The medium may be inoculated with lactic acid bacteria and cultured at 37 ° C. for 2 to 3 days. During the culture, the pressure may be reduced.

The amount of lactic acid bacteria inoculated into the starter medium is as follows:
The content is preferably 0.1 to 5% by mass, particularly preferably 0.3 to 1% by mass. The culture temperature may be any temperature at which lactic acid bacteria can grow. Specifically, 30 to 40 ° C. is good, especially 35 to 38 ° C.
Is preferred. Further, the lactic acid bacteria may be cultured until the lactic acid bacteria reach the stationary growth phase, and it is preferable to perform the culture until the pH reaches 5.0 to 3.5, particularly 4.2 to 3.8.

From the thus obtained culture, an odorless culture supernatant can be obtained by treating the culture according to a conventional method. That is, by subjecting the lactic acid bacteria culture medium to treatments such as filtration and centrifugation, it is possible to fractionate the culture supernatant having a reduced odor component such as diacetyl.

[0018] By the above procedure, odor components such as diacetyl in the culture supernatant are remarkably reduced.
In order to further reduce other aroma components and make them more preferable as cosmetic ingredients, a sparingly soluble or insoluble carbonate, or talc is added to the culture after the fermentation of lactic acid bacteria, and the mixture is stirred. It is preferable to separate and acquire the culture supernatant in the same manner.

The carbonates used for such a carbonate addition treatment include magnesium carbonate, calcium carbonate, and the like.
Zinc carbonate, nickel carbonate, barium carbonate, praseodymium carbonate, beryllium carbonate, magnesium calcium carbonate,
Radium carbonate, yttrium carbonate, cadmium carbonate, silver carbonate, chromium carbonate, cobalt carbonate, dysprosium carbonate, mercury carbonate, cerium carbonate, iron carbonate, copper carbonate, strontium carbonate, manganese carbonate, manganese carbonate, lead carbonate, etc. Calcium is preferred. The amount of carbonate added in this treatment was 0.005 relative to the culture.
% To 10%, more preferably 0.1% to 2%.
%, More preferably 0.3% to 1.2%, especially 0.4% to
1% is preferred.

The odorless culture supernatant obtained as described above may be used as it is for various products, but it is subjected to a commonly used treatment such as a heat treatment, an evaporation treatment and a reduced pressure treatment as long as the effect is not impaired. You may use it afterwards.

The odorless culture supernatant of the present invention can be prepared by a conventional method.
Cosmetic products, pharmaceuticals, quasi-drugs can be incorporated into skin external preparation compositions, such as lotions, emulsions, moisturizing creams, cleansing creams, massage creams, facial cleansers, packs, basic cosmetics such as serums, shampoos, Hair products such as rinses, treatments, hair tonics, hair liquids, hair creams and hair milks, bath cosmetics such as bath salts, makeup cosmetics such as foundations, lipsticks, mascaras, eye shadows, special cosmetics such as sunscreens, after-shave lotions, It can be in various forms such as body soap. In this case, the amount of the odorless cultured fine spirit varies depending on the form of the external preparation for skin and the like, but is usually preferably 0.1 to 80% by mass, and particularly preferably 1 to 80% by mass.
50% by mass is preferred.

The composition for external use on skin of the present invention using the above-mentioned odorless culture supernatant is produced by a commonly used production method. For example, the odorless culture supernatant of the present invention may be dissolved or dispersed in purified water, a lotion base, a cream base, an emulsion base or the like so as to exert a desired effect. At this time, in order to effectively exert the effect of the odorless culture sperm itself and the effect of reducing the fragrance component, the pH of the external preparation composition for skin is 4.0 to 10.0, particularly 4.5 to 8.0. It is desirable to adjust to. For adjusting the pH, a commonly used pH adjuster may be used, and for example, sodium hydroxide, potassium hydroxide, citric acid, malic acid and the like can be used.

In addition to the above-mentioned essential components, the skin external preparation composition of the present invention contains raw materials usually used in skin external preparations,
For example, surfactants, oils, alcohols, humectants, thickeners, preservatives, antioxidants, chelating agents, pH adjusters, fragrances, pigments, ultraviolet absorbers / scatterers, powders, vitamins, amino acids, Water-soluble polymer, foaming agent, pigment, plant extract,
Animal-derived components, seaweed extracts, various drugs, additives, water and the like can be blended.

Examples of the surfactant include sorbitan monolaurate, sorbitan monopalmitate, sorbitan sesquioleate, sorbitan trioleate, polyoxyethylene sorbitan monolaurate, polyoxethylene sorbitan monostearate, polyethylene glycol monooleate, and polyethylene glycol. Alkylate,
Polyoxyethylene alkyl ether, polyglycol diether, lauroyl diethanol amide, fatty acid isopropanol amide, maltitol hydroxy fatty acid ether, alkylated polysaccharide, alkyl glucoside,
Nonionic surfactants such as sugar esters, lipophilic glycerin monostearate, self-emulsifying glycerin monostearate, polyglycerin monostearate, polyglycerin alkylate, sorbitan monooleate, polyethylene glycol monostearate, polyoxy Nonionic surfactants such as ethylene sorbitan monooleate, polyoxyethylene cetyl ether, polyoxyethylenated sterol, polyoxyethylenated lanolin, polyoxyethylenated beeswax, polyoxyethylene hydrogenated castor oil, sodium stearate, potassium palmitate , Sodium cetyl sulfate, sodium lauryl phosphate, sodium polyoxyethylene lauryl sulfate, triethanolamine palmitate, sodium polyoxyethylene lauryl phosphate Anionic surfactants such as sodium chloride, sodium N-acylglutamate, cationic surfactants such as stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, benzalkonium chloride, laurylamine oxide, alkylaminoethylglycine hydrochloride solution, lecithin, etc. An amphoteric surfactant and the like can be exemplified.

The oils include macadamia nut oil, castor oil, olive oil, cacao oil, camellia oil, coconut oil, wood wax,
Vegetable oils such as jojoba oil, grape seed oil, avocado oil, animal oils such as mink oil, egg yolk oil, beeswax, spermaceti, lanolin, carnauba wax, candelilla wax, etc., liquid paraffin, squalane, micro Hydrocarbons such as crystallin wax, ceresin wax, paraffin wax, petrolatum, lauric acid, myristic acid, stearic acid, oleic acid, isostearic acid, behenic acid, palmitic acid, capric acid, lanolin fatty acid, linoleic acid, linolenic acid, etc. Natural and synthetic fatty acids, cetanol, stearyl alcohol, hexyldecanol, octyldodecanol, lauryl alcohol,
Examples include natural and synthetic higher alcohols such as caprylic alcohol, myristyl alcohol, cetyl alcohol, cholesterol, and phytosterol; esters such as isopropyl myristate, isopropyl palmitate, octyldodecyl myristate, octyldodecyl oleate, and cholesterol oleate. be able to.

Examples of the humectant include pentylene glycol such as glycerin, erythritol, xylitol, maltitol, propylene glycol, 1,3-butylene glycol, sorbitol, polyglycerin, polyethylene glycol, dipropylene glycol and 1,2-pentanediol. , Polyhydric alcohols such as isopropylene glycol, amino acids, natural moisturizing ingredients such as sodium lactate and sodium pyrrolidone carboxylate, xyloglucan, quince seed, carrageenan, pectin, mannan, curdlan, galactan, dermatan sulfate, glycogen, keratan sulfate , Chondroitin, mucoitin sulfate,
Water-soluble polymer substances such as keratosulfate, locust bean gum, succinoglucan, caronic acid, heparan sulfate, sodium hyaluronate, hyaluronic acid, collagen, mucopolysaccharides, and chondroitin sulfate can be exemplified.

As preservatives, benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 3,4,4'- Examples include trichlorocarbanilide, benzalkonium chloride, hinokitiol, resorcin, hexachlorophen, ethanol and the like.

As antioxidants, dibutylhydroxytoluene, butylhydroxyanisole, propyl gallate, ascorbic acid and the like, and as chelating agents, edetate, pyrophosphate, hexametaphosphate, citric acid, tartaric acid, gluconic acid Examples of the pH adjuster include sodium hydroxide, triethanolamine, citric acid, sodium citrate, boric acid, borax, potassium hydrogen phosphate, and the like.

Examples of the ultraviolet absorbing / scattering agent include a para-aminobenzoic acid-based ultraviolet absorber, an anthranilic acid-based ultraviolet absorber, a salicylic acid-based ultraviolet absorber, a cinnamic acid-based ultraviolet absorber, a benzophenone-based ultraviolet absorber, and a sugar-based ultraviolet absorber. , 3- (4'-methylbenzylidene) -d-camphor, 3-benzylidene-d, 1-camphor, urocanic acid, urocanic acid ethyl ester, 2-phenyl-5
-Methylbenzoxazole, 2- (2'-hydroxy-
5′-t-octylphenyl) benzotriazole, 2
-(2'-hydroxy-5'-methylphenyl) benzotriazole, dibenzarazine, dianisoylmethane,
4-methoxy-4'-t-butyldibenzoylmethane,
5- (3,3-dimethyl-2-norbornylidene) -3
-Pentan-2-one, 2-hydroxy-4-methoxybenzophenone, octyldimethylparaaminobenzoate, ethylhexylparamethoxycinnamate, titanium oxide, kaolin, talc and the like.

Examples of vitamins include vitamin A such as β-carotene and its derivatives, retinol and its derivatives or esters, vitamin B6 hydrochloride, vitamin B
6 tripalmitate, vitamin B6 dioctanoate,
Vitamin Bs such as vitamin B2 and its derivatives, vitamin B12, vitamin B15 and its derivatives, ascorbic acid, ascorbic acid sulfate and salts thereof,
Vitamin Cs such as ascorbic acid phosphate and salts thereof, ascorbic acid dipalmitate, ascorbic acid glucoside, acylascorbic acid glucoside, ascorbic acid tetraisopalmitate, vitamin D, α-
Vitamin Es such as tocopherol, β-tocopherol, γ-tocopherol, vitamin E acetate, vitamin F, pantothenic acid, pantethine, vitamin H, vitamin K, vitamin P, vitamin U, carnitine, ferulic acid, γ-oryzanol, α- Lipoic acid, orotic acid and their derivatives can be exemplified.

The amino acids include glycine, alanine, valine, leucine, isoleucine, serine, threonine, phenylalanine, tyrosine, asparagine, glutamine, taurine, tryptophan, cystine, cysteine, methionine, proline, hydroxyproline,
Examples include aspartic acid, glutamic acid, arginine, histidine, lysine, and derivatives thereof.

As the thickener, sodium alginate,
Natural polymer substances such as xanthan gum, aluminum silicate, quince seed extract, gum arabic, hydroxyethylene guar gum, carboxymethylene guar gum, guar gum, dextran, tragacanth gum, starch, chitin, chitosan, carboxymethyl chitin, agar, cellulose, hydroxypropyl Semi-synthetic polymers such as cellulose, methylhydroxypropylcellulose, methylcellulose, carboxymethylcellulose, hydroxyethylcellulose, soluble starch, cationized cellulose, carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, vinyl alcohol / vinyl acetate copolymer, acrylic acid Synthetic polymer substances such as alkyl methacrylate copolymers can be exemplified.

As plant extracts, Rye, Clara,
Kouhone, orange, sage, yarrow, mallow, assembly, thyme, touki, spruce, birch, horsetail, loofah, marronnier, yukinoshita, arnica, lily,
Examples include extracts from mugworts, peonies, aloe, gardenia, sawara, bokuryo, sanshishi, ougon, licorice, kawagomugi, kujin, yokuinin, nindou, peonies, sakuhaku, hawthorn, buttonpi, cepharanthin, and the like.

The seaweed extract includes brown algae, red algae, green algae,
There are extracts from blue-green algae, and specifically, extracts from kelp, makombu, wakame, hijiki, tengusa, coral, palmaria, tsunomata, nori, aosa, anaoaosa, ascophyllum, hibamata, mozuku, okinawamozuku, himantaria, etc. Can be exemplified.

Various drugs include nicotinamide, benzyl nicotinate, γ-oryzanol, allantoin,
Glycyrrhizic acid and its salts, glycyrrhetinic acid and its derivatives, hinokitiol, bisabolol, eucalptone, thymol, inositol, saikosaponin, carrot saponin, saponins such as hetimasaponin, muclodisaponin, pantothenyl ethyl ether, ethinyl estradiol, tranexamic acid , Arbutin, placenta extract and the like.

[0036]

The present invention will be described in more detail with reference to the following Test Examples and Examples, which should not be construed as limiting the present invention.

Test Example 1 Production of lactic acid culture supernatant: a medium obtained by heat-sterilizing an 8% aqueous solution of skim milk powder (hereinafter referred to as "aerobic medium") and a medium in which nitrogen gas was injected until the medium was saturated. (Hereinafter referred to as “nitrogen-substituted medium”). Each of the two types of culture media thus obtained was applied to Streptococcus thermophilus YIT2001 (FERM P-118).
91) of the starter was inoculated at 1%. Thereafter, the medium into which nitrogen gas had been injected was again injected with nitrogen gas to expel air, and then sealed. Then, both cultures were stirred and cultured at 37 ° C. for 24 hours and then statically cultured for 48 hours. After completion of the culture, the mixture was filtered with a filter.

After filtration, each medium was further divided into three equal parts, and one was used as it was as a culture supernatant. One of the remaining is heated under reduced pressure at 65 ° C., and is subjected to a step of concentrating until the total amount of the medium is reduced by 10% (hereinafter, referred to as “dealing treatment”). Used as Qing. The other is to add magnesium carbonate to a final concentration of 0.5%, stir, filter and filter (hereinafter referred to as “charcoal treatment”), and then use it as a culture supernatant. Using.

The culture supernatant of the nitrogen-substituted medium thus obtained (hereinafter referred to as "nitrogen-substituted SE") and the culture supernatant of the aerobic medium (hereinafter referred to as "aerobic culture SE"). .), And each of the dealt-treated culture supernatant and the charcoal-mag treated culture supernatant was taken into a flask, and 50 mL
After the temperature was kept at a constant temperature of ° C., the upper atmosphere was sampled and odor components were quantified by gas chromatography. Table 1 shows the results.

[0040]

[Table 1]

From the results shown in Table 1, the amount of diacetyl was reduced by about 95% by the nitrogen substitution treatment, and a reduction effect was recognized. In addition, the combined use of the nitrogen replacement treatment and the treatment with the coal mag treatment alone were more effective than the dealt treatment and the charcoal mag treatment alone, and the combined use with the charcoal mag treatment was particularly effective.

Example 1 Toner (1): Streptococcus thermophilus was used as a lactic acid bacteria starter, and nitrogen-substituted SE was obtained in the same manner as in Test Example 1 except for the above. Using this nitrogen-substituted SE, a lotion having the following composition was prepared from the following formulation. This lotion was prepared by first mixing other components except 1,3-butylene glycol and sodium citrate, and then adjusting the pH to 6.0 with sodium hydroxide.
Thereafter, the above components were mixed and produced.

(Prescription) (%) Brusin denatured alcohol 10 Nitrogen-substituted SE 10 or 20 1,3-butylene glycol 2 Sodium citrate Appropriate amount Purified water residue

Comparative Example 1 Lotion (2): A lotion was prepared in the same manner as in Example 1 except that the nitrogen-substituted SE in Example 1 was changed to aerobic culture SE.

Test Example 2 Feeling of Use Test: The lotion obtained in Example 1 and Comparative Example 2 was applied to the skin for 12 professional panels,
The usability test was conducted by giving a score based on the measurement method and judgment criteria described below. Table 2 shows the results.

<Measurement Method> Moisturizing property: The lotion obtained as described above was applied to the inside of the forearm of a person (5 persons) having dry skin. The measurement was performed using a meter (Skicon 200 type; manufactured by IBS). The same experiment was performed three times, and the average value of the water content of the skin was determined.

PH adjusting action: 1,1
The amount (m) of sodium hydroxide solution (0.1 mol / L) required to adjust 10 ml of the solution excluding 3-butylene glycol and sodium citrate to pH 6.0.
L) was determined.

<Judgment Criteria> Rating: Evaluation +3: No fermentation odor. +2: Neither can be said. +1: There is a fermentation smell.

[0049]

[Table 2]

From the results shown in Table 2, it is found that the lotion using the aerobic culture SE has a blending amount of 10% or more capable of maintaining the moisturizing property and the pH adjusting effect, and 10 to 12 panelists (80 to 100 of the total) %) Felt unpleasant odor, whereas most lots of panelists did not feel unpleasant odor in the lotion using nitrogen-substituted SE even if the blending amount was 20%.

Example 2 Cream: A cream having the following composition was produced by a conventional method. The culture supernatant used had been subjected to a dealate treatment with the nitrogen-substituted medium prepared in Example 1.

(Formulation) (%) Squalane 10 Cetyl 2-ethylhexanoate 10 Purified jojoba oil 2 Cholesteryl hydroxystearate 3 Batyl stearate 2 Behenyl alcohol 2 Stearyl glycyrrhetinate 0.1 Glyceryl monostearate 3 POE (20) Sorbitan mono Stearate 2 Lactic acid bacteria culture supernatant 3 Sodium hyaluronate 0.02 1,3-butylene glycol 5 Methylparaben 0.2 Disodium edetate 0.1 Sodium hydroxide Appropriate amount Extract powder of liquorice 0.05 Hawthorn extract 2.0 Hawthorn extract 2 2.0 Mulberry Extract 2.0 Touki Extract 2.0 Purified Water Residue

Example 3 Lotion: A lotion having the following composition was produced by a conventional method. The culture supernatant used had been dealtated with the nitrogen-substituted medium prepared in Example 1.

(Prescription) (%) Culture supernatant of lactic acid bacteria 30 ethanol 5 POE (40) hydrogenated castor oil 0.1 dipotassium glycyrrhetinate 0.1 disodium edetate 0.1 1,3-butylene glycol 0.5 methyl paraben 0 .15 Sodium hyaluronate 0.01 1,2-Pentanediol 2.0 Sodium hydroxide Appropriate amount Peony extract 0.5 Clara extract 0.5 Yokuinin extract 0.5 Purified water residue

Example 4 Cleansing Cream: A cleansing cream having the following composition was produced by a conventional method. The culture supernatant used had been dealtated with the nitrogen-substituted medium prepared in Example 1.

(Formulation) (%) Liquid paraffin 40 Octyldodecyl myristate 10 Isostearic acid 1 Vaseline 7 Stearic acid 1 Behenyl alcohol 2 Beeswax 2 Stearyl glycyrrhetinate 0.1 POE (40) Monostearate 0.1 POE (20) Sorbitan Monostearate 2 Methyl paraben 0.05 1,3-butylene glycol 8 1,2-Pentanediol 2 Acrylic acid / alkyl methacrylate copolymer 0.5 Disodium edetate 0.05 Culture supernatant of lactic acid bacteria 5 Sodium hyaluronate 0 0.001 Licorice extract powder 0.05 Clara extract 1.5 Honeysuckle extract 1.5 Purified water Residue

Example 5 Emulsion: An emulsion having the following composition was prepared by a conventional method.
The culture supernatant used had been dealtated with the nitrogen-substituted medium prepared in Example 1.

(Formulation) (%) Squalane 5 Jojoba oil 2 Octyldodecyl myristate 2 Cholesteryl hydroxystearate 0.2 Batyl stearate 0.5 Behenyl alcohol 0.3 Stearic acid 0.7 Behenic acid 0.4 Glyceryl monostearate 2.5 Stearyl glycyrrhetinate 0.1 POE (20) Sorbitan monostearate 1.2 Lactic acid bacteria culture supernatant 15 Disodium edetate 0.1 Sodium hydroxide Appropriate amount 1,3-butylene glycol 8 Glycerin 2 Sodium hyaluronate 0.0 001 Methyl paraben 0.15 Licorice extract powder 0.1 Mulberry extract 1.0 Ougon extract 1.0 Touki extract 1.0 Purified water Residue

When the same use feeling test as in Test Example 2 was performed on the skin external preparation compositions obtained from Examples 2 to 5, any of the skin external preparation compositions had no fermentation smell and had high palatability. It turned out to be.

[0060]

According to the present invention, it is possible to provide a culture supernatant of a lactic acid bacterium in which a characteristic aroma component produced by lactic acid bacterium fermentation is reduced.

As described above, the culture supernatant of lactic acid bacteria obtained by the present invention has reduced peculiar odor components felt as a fermentation odor. Lotion, milk lotion, moisturizing cream, cleansing cream, etc. which have effects such as moisturizing action, antioxidant action, photoprotective action, control action of skin flora, control action of skin pH etc. which the lactic acid bacteria culture supernatant itself has few problems. Massage cream, facial cream, pack,
It is advantageously used as various skin external preparation compositions such as a serum. that's all

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61P 17/16 A61P 17/16 31/04 31/04 39/06 39/06 C12N 1/20 C12N 1 / 20 E // (C12P 1/04 (C12P 1/04 Z C12R 1:46) C12R 1:46) (72) Inventor Takashi Kinoshita 1-1-1 Higashishimbashi, Minato-ku, Tokyo Yakult Honsha F-term (reference) 4B064 AH19 CA02 CC09 CC12 CE03 DA01 DA20 4B065 AA30X AA49X AC14 BB24 BC01 BC14 BC26 BC50 BD14 CA50 CA60 4C083 AA032 AA082 AA112 AA122 AC012 AC022 AC102 AC112 AC122 AC172 AC532 AD402 AC402 AC402 AC332 CC02 CC04 CC05 CC23 DD23 DD31 EE12 EE17 4C087 AA01 AA02 BC56 BC57 BC58 BC61 CA10 MA63 NA09 ZA89 ZB35

Claims (6)

[Claims] 1. After inoculating a lactic acid bacterium into a medium containing milk as a main component, the medium is cultured under replacement of an inert gas, and a fragrance component obtained by separating a supernatant from the obtained lactic acid bacterium culture is obtained. Reduced lactic acid bacteria culture supernatant. 2. A method for producing a lactic acid bacterium culture supernatant in which a lactic acid bacterium is cultured by inoculating a lactic acid bacterium into a medium containing milk as a main component and a supernatant is obtained from the obtained lactic acid bacterium culture. A method for producing a culture supernatant of a lactic acid bacterium having reduced aroma components, which is performed under gas replacement. 3. The method according to claim 2, wherein the lactic acid bacteria culture obtained by culturing under inert gas replacement is further treated with a hardly soluble or insoluble carbonate or talc. A method for producing a culture supernatant of a lactic acid bacterium having reduced aroma components. 4. The method for producing a culture supernatant of a lactic acid bacterium having a reduced odor component according to claim 2, wherein the odor component is diacetyl. 5. The method according to claim 5, wherein the lactic acid bacteria are Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus acidophilus.
The flavor component according to any one of claims 2 to 4, which is one or more of lactic acid bacteria selected from casei, Lactococcus lactis, Streptococcus lactis, Streptococcus thermophilus. Method for producing lactic acid bacteria culture supernatant 6. A composition for external use on the skin, comprising the culture supernatant of a lactic acid bacterium having a low odor component according to claim 1.