JP2001524307A - ヒト椎間円板細胞の調製方法 - Google Patents

ヒト椎間円板細胞の調製方法

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Publication number
JP2001524307A
JP2001524307A JP2000522219A JP2000522219A JP2001524307A JP 2001524307 A JP2001524307 A JP 2001524307A JP 2000522219 A JP2000522219 A JP 2000522219A JP 2000522219 A JP2000522219 A JP 2000522219A JP 2001524307 A JP2001524307 A JP 2001524307A
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Prior art keywords
cells
cell
growth factor
disc
insulin
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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JP2000522219A
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English (en)
Japanese (ja)
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JP2001524307A5 (https=
Inventor
ヘインリー，エドワード・ナサニエル，ジュニア
グルーバー，エリザベス・ヘレン
Original Assignee
シャーロット‐メクレンバーグ・ホスピタル・オーソリティ
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Publication of JP2001524307A publication Critical patent/JP2001524307A/ja
Publication of JP2001524307A5 publication Critical patent/JP2001524307A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2000522219A 1997-11-26 1998-11-24 ヒト椎間円板細胞の調製方法 Pending JP2001524307A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/979,674 US6080579A (en) 1997-11-26 1997-11-26 Method for producing human intervertebral disc cells
US08/979,674 1997-11-26
PCT/US1998/025137 WO1999027077A1 (en) 1997-11-26 1998-11-24 Method for producing human intervertebral disc cells

Publications (2)

Publication Number Publication Date
JP2001524307A true JP2001524307A (ja) 2001-12-04
JP2001524307A5 JP2001524307A5 (https=) 2005-06-23

Family

ID=25527059

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000522219A Pending JP2001524307A (ja) 1997-11-26 1998-11-24 ヒト椎間円板細胞の調製方法

Country Status (5)

Country Link
US (2) US6080579A (https=)
JP (1) JP2001524307A (https=)
AU (1) AU1604599A (https=)
CA (1) CA2311763C (https=)
WO (1) WO1999027077A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010532994A (ja) * 2007-07-12 2010-10-21 ディスクジェニクス ヒト円板組織

Families Citing this family (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0896825B1 (en) * 1997-08-14 2002-07-17 Sulzer Innotec Ag Composition and device for in vivo cartilage repair comprising nanocapsules with osteoinductive and/or chondroinductive factors
US7101545B1 (en) * 1997-11-26 2006-09-05 Charlotte-Mecklenburg Hospital Authority Method for using human intervertebral disc cells
US6080579A (en) * 1997-11-26 2000-06-27 Charlotte-Mecklenburg Hospital Authority Method for producing human intervertebral disc cells
MXPA01003538A (es) * 1998-10-06 2005-09-08 Rush Presbyterian St Luke Metodo para el tratamiento de quimionucleolisis.
US6179840B1 (en) 1999-07-23 2001-01-30 Ethicon, Inc. Graft fixation device and method
US20020095157A1 (en) 1999-07-23 2002-07-18 Bowman Steven M. Graft fixation device combination
US20030004574A1 (en) * 1999-10-08 2003-01-02 Ferree Bret A. Disc and annulus augmentation using biologic tissue
US6648919B2 (en) * 1999-10-14 2003-11-18 Bret A. Ferree Transplantation of engineered meniscus tissue to the intervertebral disc
JP3261456B2 (ja) * 1999-10-29 2002-03-04 独立行政法人産業技術総合研究所 細胞培養担体及び該担体を用いた細胞の培養方法
US6723335B1 (en) 2000-04-07 2004-04-20 Jeffrey William Moehlenbruck Methods and compositions for treating intervertebral disc degeneration
CA2413353C (en) * 2000-06-29 2013-01-29 Rita Kandel Intervertebral disc
US6852330B2 (en) 2000-12-21 2005-02-08 Depuy Mitek, Inc. Reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
CA2365376C (en) * 2000-12-21 2006-03-28 Ethicon, Inc. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
US6599323B2 (en) * 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
US20020127265A1 (en) * 2000-12-21 2002-09-12 Bowman Steven M. Use of reinforced foam implants with enhanced integrity for soft tissue repair and regeneration
US7008433B2 (en) 2001-02-15 2006-03-07 Depuy Acromed, Inc. Vertebroplasty injection device
JP4955388B2 (ja) * 2001-08-31 2012-06-20 ユニヴァーシティー オブ サザン カリフォルニア 脊椎椎間板疾患を治療するための架橋試薬
AU2002335747B2 (en) * 2001-09-15 2009-01-29 Rush University Medical Center Stratified cartilage tissue and methods to engineer same
US20030165473A1 (en) * 2001-11-09 2003-09-04 Rush-Presbyterian-St. Luke's Medical Center Engineered intervertebral disc tissue
US7232802B2 (en) 2001-12-21 2007-06-19 Zimmer Orthobiologics, Inc. Compositions and methods for promoting myocardial and peripheral angiogenesis
US20030138873A1 (en) * 2002-01-22 2003-07-24 Koichi Masuda Tissue engineered cartilage for drug discovery
WO2003066120A1 (en) * 2002-02-04 2003-08-14 Ferree Bret A Treating degenerative disc disease through transplantation of allograft disc
US7622562B2 (en) 2002-06-26 2009-11-24 Zimmer Orthobiologics, Inc. Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
US20040054414A1 (en) 2002-09-18 2004-03-18 Trieu Hai H. Collagen-based materials and methods for augmenting intervertebral discs
US7744651B2 (en) * 2002-09-18 2010-06-29 Warsaw Orthopedic, Inc Compositions and methods for treating intervertebral discs with collagen-based materials
US20040078090A1 (en) 2002-10-18 2004-04-22 Francois Binette Biocompatible scaffolds with tissue fragments
US7824701B2 (en) * 2002-10-18 2010-11-02 Ethicon, Inc. Biocompatible scaffold for ligament or tendon repair
JP2006515765A (ja) * 2002-11-15 2006-06-08 エスディージーアイ・ホールディングス・インコーポレーテッド 滑膜性関節を治療するためのコラーゲンベース材料および方法
US20040186471A1 (en) * 2002-12-07 2004-09-23 Sdgi Holdings, Inc. Method and apparatus for intervertebral disc expansion
JP2006518256A (ja) * 2003-01-31 2006-08-10 ジンマー オーソバイオロジクス，インコーポレイティド 髄核組織（ｎｕｃｌｅｕｓｐｕｌｐｏｓｕｓｔｉｓｓｕｅ）を含むヒドロゲル組成物
US20060015182A1 (en) * 2003-02-25 2006-01-19 Tsou Paul M Patch material for intervertebral disc annulus defect repair
US8197837B2 (en) 2003-03-07 2012-06-12 Depuy Mitek, Inc. Method of preparation of bioabsorbable porous reinforced tissue implants and implants thereof
AU2003214708A1 (en) 2003-03-14 2004-09-30 Roque Humberto Ferreyro Irigoyen Hydraulic device for the injection of bone cement in percutaneous vertebroplasty
US8066713B2 (en) 2003-03-31 2011-11-29 Depuy Spine, Inc. Remotely-activated vertebroplasty injection device
WO2006011152A2 (en) 2004-06-17 2006-02-02 Disc-O-Tech Medical Technologies, Ltd. Methods for treating bone and other tissue
US8415407B2 (en) 2004-03-21 2013-04-09 Depuy Spine, Inc. Methods, materials, and apparatus for treating bone and other tissue
US8226715B2 (en) 2003-06-30 2012-07-24 Depuy Mitek, Inc. Scaffold for connective tissue repair
US10583220B2 (en) 2003-08-11 2020-03-10 DePuy Synthes Products, Inc. Method and apparatus for resurfacing an articular surface
WO2005030034A2 (en) 2003-09-26 2005-04-07 Depuy Spine, Inc. Device for delivering viscous material
US7316822B2 (en) 2003-11-26 2008-01-08 Ethicon, Inc. Conformable tissue repair implant capable of injection delivery
US7901461B2 (en) 2003-12-05 2011-03-08 Ethicon, Inc. Viable tissue repair implants and methods of use
US7824711B2 (en) 2003-12-11 2010-11-02 Isto Technologies, Inc. Particulate cartilage system
US8148150B2 (en) * 2003-12-12 2012-04-03 Co.Don Ag Method for the production of intervertebral disk cell transplants and their use as transplantation material
US11395865B2 (en) * 2004-02-09 2022-07-26 DePuy Synthes Products, Inc. Scaffolds with viable tissue
US8657881B2 (en) * 2004-04-20 2014-02-25 Depuy Mitek, Llc Meniscal repair scaffold
US8221780B2 (en) 2004-04-20 2012-07-17 Depuy Mitek, Inc. Nonwoven tissue scaffold
US8137686B2 (en) 2004-04-20 2012-03-20 Depuy Mitek, Inc. Nonwoven tissue scaffold
EP1614402A1 (en) * 2004-07-09 2006-01-11 Paul M. Tsou Patch material for inervertebral disc annulus defect repair
US8697139B2 (en) 2004-09-21 2014-04-15 Frank M. Phillips Method of intervertebral disc treatment using articular chondrocyte cells
US9381024B2 (en) 2005-07-31 2016-07-05 DePuy Synthes Products, Inc. Marked tools
US9918767B2 (en) 2005-08-01 2018-03-20 DePuy Synthes Products, Inc. Temperature control system
JP5292533B2 (ja) 2005-08-26 2013-09-18 ジンマー・インコーポレイテッド インプラントおよび関節疾患の治療、置換および治療方法
US8360629B2 (en) 2005-11-22 2013-01-29 Depuy Spine, Inc. Mixing apparatus having central and planetary mixing elements
US9598673B2 (en) * 2006-05-19 2017-03-21 Creative Medical Health Treatment of disc degenerative disease
US20080004703A1 (en) * 2006-06-30 2008-01-03 Warsaw Orthopedic, Inc. Method of treating a patient using a collagen material
US8399619B2 (en) * 2006-06-30 2013-03-19 Warsaw Orthopedic, Inc. Injectable collagen material
US20080004431A1 (en) * 2006-06-30 2008-01-03 Warsaw Orthopedic Inc Method of manufacturing an injectable collagen material
US8118779B2 (en) * 2006-06-30 2012-02-21 Warsaw Orthopedic, Inc. Collagen delivery device
US8048618B2 (en) * 2006-07-26 2011-11-01 The University Of Hong Kong Method for preparing and preserving a natural allograft intervertebral disk for humans
CA2663447A1 (en) 2006-09-14 2008-03-20 Depuy Spine, Inc. Polymeric bone cement and methods of use thereof
CA2665995C (en) 2006-10-19 2011-11-29 Oren Globerman Fluid delivery system
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US9827023B2 (en) 2007-01-29 2017-11-28 Life Spine, Inc. Craniospinal fusion method and apparatus
US8556939B2 (en) * 2008-01-08 2013-10-15 Fraser Cummins Henderson Mathematical relationship of strain, neurological dysfunction and abnormal behavior resulting from neurological dysfunction of the brainstem
US20090036894A1 (en) * 2007-01-29 2009-02-05 Polaris Biotechnology, Inc. Method of treating a neurological condition through correction and stabilization of the clivo-axial angle
US8182511B2 (en) * 2007-01-29 2012-05-22 Polaris Biotechnology, Inc. Craniospinal fusion method and apparatus
US8403965B2 (en) * 2007-01-29 2013-03-26 Polaris Biotechnology, Inc. Vertebra attachment method and system
US8043342B2 (en) 2007-01-29 2011-10-25 Polaris Biotechnology, Inc. Craniospinal fusion method and apparatus
AU2008240191B2 (en) 2007-04-12 2013-09-19 Zimmer, Inc. Compositions and methods for tissue repair
WO2009089395A2 (en) * 2008-01-08 2009-07-16 Polaris Biotechnology, Inc. Osteointegration apparatus
WO2010027789A2 (en) 2008-08-25 2010-03-11 University Of South Florida Volume exclusion agent to enhance formation of extracelluar matrix
CA2744445C (en) * 2008-11-25 2023-10-24 Tissuegene, Inc. Primed cell therapy
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4485097A (en) * 1982-05-26 1984-11-27 Massachusetts Institute Of Technology Bone-equivalent and method for preparation thereof
US5266476A (en) * 1985-06-18 1993-11-30 Yeda Research & Development Co., Ltd. Fibrous matrix for in vitro cell cultivation
US5032508A (en) * 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5496722A (en) * 1988-06-30 1996-03-05 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Method for producing non-neoplastic, three dimensional, mammalian tissue and cell aggregates under microgravity culture conditions and the products produced therefrom
US5155034A (en) * 1988-06-30 1992-10-13 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Three-dimensional cell to tissue assembly process
US5422340A (en) * 1989-09-01 1995-06-06 Ammann; Arthur J. TGF-βformulation for inducing bone growth
FR2654625B1 (fr) * 1989-11-22 1992-02-21 Transphyto Sa Procede de fabrication d'un materiau pour osteoplastie a partir d'un tissu osseux naturel et materiau obtenu par ce procede.
US5256418A (en) * 1990-04-06 1993-10-26 Organogenesis, Inc. Collagen constructs
US5964807A (en) * 1996-08-08 1999-10-12 Trustees Of The University Of Pennsylvania Compositions and methods for intervertebral disc reformation
WO1999004720A1 (en) * 1997-07-11 1999-02-04 Reprogenesis Inc. Repair of intervertebral disks
US7101545B1 (en) * 1997-11-26 2006-09-05 Charlotte-Mecklenburg Hospital Authority Method for using human intervertebral disc cells
US6080579A (en) 1997-11-26 2000-06-27 Charlotte-Mecklenburg Hospital Authority Method for producing human intervertebral disc cells
US6340369B1 (en) 1999-08-13 2002-01-22 Bret A. Ferree Treating degenerative disc disease with harvested disc cells and analogues of the extracellular matrix

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010532994A (ja) * 2007-07-12 2010-10-21 ディスクジェニクス ヒト円板組織
US9487753B2 (en) 2007-07-12 2016-11-08 Discgenics Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
US11168305B2 (en) 2007-07-12 2021-11-09 Discgenics, Inc. Methods for the treatment of degenerative disc disease

Also Published As

Publication number Publication date
CA2311763C (en) 2014-01-21
CA2311763A1 (en) 1999-06-03
US20050232903A1 (en) 2005-10-20
WO1999027077A1 (en) 1999-06-03
US7445776B2 (en) 2008-11-04
AU1604599A (en) 1999-06-15
US6080579A (en) 2000-06-27

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