JP2001502543A - 組換えヘモグロビン調製物においてプロトポルフィリンixのレベルを減少させる方法 - Google Patents
組換えヘモグロビン調製物においてプロトポルフィリンixのレベルを減少させる方法Info
- Publication number
- JP2001502543A JP2001502543A JP10519561A JP51956198A JP2001502543A JP 2001502543 A JP2001502543 A JP 2001502543A JP 10519561 A JP10519561 A JP 10519561A JP 51956198 A JP51956198 A JP 51956198A JP 2001502543 A JP2001502543 A JP 2001502543A
- Authority
- JP
- Japan
- Prior art keywords
- hemoglobin
- pix
- gene
- hemh
- protoporphyrin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.組換えヘモグロビンの産生において、プロトポルフィリンIX(PIX)含有ヘモ グロビンを減少させる方法であって、宿主細胞においてhemII遺伝子を過剰発現 させる工程を包含する、方法。 2.前記hemH遺伝子の過剰発現が、hemII遺伝子の発現を増加させるのに十分で あるように、プロモーターを前記宿主細胞に挿入することによって達成される、 請求項1に記載の方法。 3.前記プロモーターが、Ptac16、Ptac17、Plac、PlacUV5、またはPlac(down )である、請求項2に記載の方法。 4.前記プロモーターがPtacプロモーターである、請求項3に記載の方法。 5.前記hemH遺伝子が、天然に存在するhemH遺伝子の変異体である、請求項1〜 4に記載の方法。 6.前記hemH遺伝子の過剰発現が、該hemH遺伝子の複数のコピーを前記宿主細胞 に挿入することによって達成される、請求項1〜5に記載の方法。 7.前記hemH遺伝子の1コピーを、前記宿主細胞の染色体に組み込んで該hemH遺 伝子の発現を増加させる、請求項1に記載の方法。 8.前記宿主細胞が細菌細胞である、請求項1および7に記載の方法。 9.前記細菌細胞がE.coliである、請求項8に記載の方法。 10.前記PIXが、hemH遺伝子の過剰発現を伴わない組換えヘモグロビンの産生 と比較して、少なくとも50%減少される、請求項1に記載の方法。 11.該溶液中に約10%未満のPIX含有ヘモグロビンを有する、実質的にPIXを含 まないヘモグロビン溶液。 12.前記溶液が、前記溶液中に約6%未満のPIX含有ヘモグロビンを含む、請 求項11に記載のヘモグロビン溶液。 13.前記溶液が、前記溶液中に約1%未満のPIX含有ヘモグロビンを含む、請 求項11に記載のヘモグロビン溶液。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2851696P | 1996-10-18 | 1996-10-18 | |
US60/028,516 | 1996-10-18 | ||
PCT/US1997/018950 WO1998017809A1 (en) | 1996-10-18 | 1997-10-17 | Methods of reducing the levels of protoporphyrin ix in recombinant hemoglobin preparations |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001502543A true JP2001502543A (ja) | 2001-02-27 |
JP2001502543A5 JP2001502543A5 (ja) | 2005-06-16 |
Family
ID=21843890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10519561A Ceased JP2001502543A (ja) | 1996-10-18 | 1997-10-17 | 組換えヘモグロビン調製物においてプロトポルフィリンixのレベルを減少させる方法 |
Country Status (6)
Country | Link |
---|---|
US (1) | US6136565A (ja) |
EP (1) | EP0954586A1 (ja) |
JP (1) | JP2001502543A (ja) |
AU (1) | AU4990697A (ja) |
CA (1) | CA2268278A1 (ja) |
WO (1) | WO1998017809A1 (ja) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0954586A1 (en) * | 1996-10-18 | 1999-11-10 | Baxter Biotech Technology S.A.R.L. | Methods of reducing the levels of protoporphyrin ix in recombinant hemoglobin preparations |
GB0000908D0 (en) * | 2000-01-14 | 2000-03-08 | Scient Generics Ltd | Parallel free-space optical communications |
US20050009029A1 (en) * | 2001-12-12 | 2005-01-13 | Frye Christopher Carl | Expression system |
DE10224750A1 (de) | 2002-06-04 | 2003-12-24 | Fresenius Medical Care De Gmbh | Vorrichtung zur Behandlung einer medizinischen Flüssigkeit |
CA2500741C (en) | 2002-10-04 | 2017-02-28 | Genencor International, Inc. | Glucose transport mutants for production of biomaterial |
US7935074B2 (en) | 2005-02-28 | 2011-05-03 | Fresenius Medical Care Holdings, Inc. | Cassette system for peritoneal dialysis machine |
US8197231B2 (en) | 2005-07-13 | 2012-06-12 | Purity Solutions Llc | Diaphragm pump and related methods |
MX2011003574A (es) * | 2008-10-03 | 2011-08-12 | Charlotte Mecklenburg Hospital | Tratamiento de la infeccion hepatitis c con metaloporfirinas. |
US8192401B2 (en) | 2009-03-20 | 2012-06-05 | Fresenius Medical Care Holdings, Inc. | Medical fluid pump systems and related components and methods |
EP2453946B1 (en) | 2009-07-15 | 2013-02-13 | Fresenius Medical Care Holdings, Inc. | Medical fluid cassettes and related systems |
US8720913B2 (en) | 2009-08-11 | 2014-05-13 | Fresenius Medical Care Holdings, Inc. | Portable peritoneal dialysis carts and related systems |
DE102010053973A1 (de) | 2010-12-09 | 2012-06-14 | Fresenius Medical Care Deutschland Gmbh | Medizinisches Gerät mit einer Heizung |
US9694125B2 (en) | 2010-12-20 | 2017-07-04 | Fresenius Medical Care Holdings, Inc. | Medical fluid cassettes and related systems and methods |
US9624915B2 (en) | 2011-03-09 | 2017-04-18 | Fresenius Medical Care Holdings, Inc. | Medical fluid delivery sets and related systems and methods |
EP3006059B1 (en) | 2011-04-21 | 2017-09-27 | Fresenius Medical Care Holdings, Inc. | Medical fluid pumping systems and related devices and methods |
US9186449B2 (en) | 2011-11-01 | 2015-11-17 | Fresenius Medical Care Holdings, Inc. | Dialysis machine support assemblies and related systems and methods |
US9610392B2 (en) | 2012-06-08 | 2017-04-04 | Fresenius Medical Care Holdings, Inc. | Medical fluid cassettes and related systems and methods |
US9500188B2 (en) | 2012-06-11 | 2016-11-22 | Fresenius Medical Care Holdings, Inc. | Medical fluid cassettes and related systems and methods |
US9561323B2 (en) | 2013-03-14 | 2017-02-07 | Fresenius Medical Care Holdings, Inc. | Medical fluid cassette leak detection methods and devices |
US10117985B2 (en) | 2013-08-21 | 2018-11-06 | Fresenius Medical Care Holdings, Inc. | Determining a volume of medical fluid pumped into or out of a medical fluid cassette |
WO2017100376A2 (en) | 2015-12-07 | 2017-06-15 | Zymergen, Inc. | Promoters from corynebacterium glutamicum |
US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
US10544411B2 (en) | 2016-06-30 | 2020-01-28 | Zymergen Inc. | Methods for generating a glucose permease library and uses thereof |
JP2019519242A (ja) | 2016-06-30 | 2019-07-11 | ザイマージェン インコーポレイテッド | 細菌ヘモグロビンライブラリーを生成するための方法およびその使用 |
WO2022098745A1 (en) | 2020-11-03 | 2022-05-12 | Indi Molecular, Inc. | Compositions, delivery systems, and methods useful in tumor therapy |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2050601C (en) * | 1989-05-10 | 2000-09-19 | Stephen J. Hoffman | Production in bacteria and yeast of hemoglobin and analogues thereof |
US5545727A (en) * | 1989-05-10 | 1996-08-13 | Somatogen, Inc. | DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin |
US5665829A (en) * | 1990-02-08 | 1997-09-09 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Gt. Britain & Northern Ireland | Process for the polymerization of olefinic-containing monomers employing anionic initiators |
US5840851A (en) * | 1993-07-23 | 1998-11-24 | Plomer; J. Jeffrey | Purification of hemoglobin |
US5665869A (en) * | 1993-11-15 | 1997-09-09 | Somatogen, Inc. | Method for the rapid removal of protoporphyrin from protoporphyrin IX-containing solutions of hemoglobin |
EP0954586A1 (en) * | 1996-10-18 | 1999-11-10 | Baxter Biotech Technology S.A.R.L. | Methods of reducing the levels of protoporphyrin ix in recombinant hemoglobin preparations |
-
1997
- 1997-10-17 EP EP97912813A patent/EP0954586A1/en not_active Withdrawn
- 1997-10-17 AU AU49906/97A patent/AU4990697A/en not_active Abandoned
- 1997-10-17 JP JP10519561A patent/JP2001502543A/ja not_active Ceased
- 1997-10-17 US US09/269,584 patent/US6136565A/en not_active Expired - Lifetime
- 1997-10-17 CA CA002268278A patent/CA2268278A1/en not_active Abandoned
- 1997-10-17 WO PCT/US1997/018950 patent/WO1998017809A1/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0954586A1 (en) | 1999-11-10 |
US6136565A (en) | 2000-10-24 |
AU4990697A (en) | 1998-05-15 |
CA2268278A1 (en) | 1998-04-30 |
WO1998017809A1 (en) | 1998-04-30 |
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Legal Events
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