JP2001302454A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To improve the unhealthy condition of the turnover of skin corneum and improve the aging of the skin. SOLUTION: This cosmetic characterized by containing the fine particles of amorphous hydroxyapatite.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a cosmetic containing amorphous hydroxyapatite fine particles.

[0002]

2. Description of the Related Art Conventionally, hydroxyapatite has been
It has been known that it is excellent in the ability to adsorb and remove sebum and waste products, has no biological irritation, and is highly safe, so that it is blended as a component of cosmetics. For example,
No. 3 discloses a cosmetic product which has a good feeling in use and can reduce skin disorders by blending spherical hydroxyapatite as a pigment with the cosmetic product. Japanese Patent Publication No. Hei 6-92288 discloses that a compound powder of hydroxyapatite is added to cosmetics to provide a good feeling in use, to prevent rough skin, and to suppress skin inflammation of acne patients. Agents and cosmetics are disclosed.

[0003] Japanese Patent Application Laid-Open No. 5-25458 discloses Me
(0.5m + 1.5n) (X) m · (PO ₄ ) n [where Me is Ca, Mg, Ba, Zn or Ce, X is O
H, Cl or F, n and m are coefficients], and Me
/ P is a poorly soluble basic phosphate having a composition of 1.4 to 1.8, and having a mean particle size in the range of 0.1 to 5 μm. It is disclosed that it can be blended as a component of cosmetics or the like as an ultraviolet absorber for improving stability. Also, Japanese Patent Application Laid-Open No. 9-40408 discloses a method for producing a large hydroxyapatite plate-like crystal in order to improve covering power for cosmetics.

[0004]

The division of normal skin epidermal cells is performed in basal cells, and the cells resulting from the division undergo differentiation processes, from spiny cells to granule cells, and to the skin surface layer. It is known to be a keratinocyte.
Normally, the process of proliferation and differentiation (hereinafter referred to as “turnover”) of these epidermal cells involves calcium ion, cyclic A
MP, protein kinase, retinoid, vitamin D3,
In addition to physiologically active substances such as glucocorticoids and prostaglandins, epidermal growth factor (EGF) and tran
Various types of cell growth factors, such as sforming growth factor β (TGF-β), are thought to be involved.

[0005] In the case of keratosis in which a certain kind of stimulus is applied to the skin and abnormal growth changes occur in the epidermis, the cells are in a dead state, and the enucleated keratinocytes are unable to lose their nuclei without failing. It is said to form a complete stratum corneum and is observed in winter when the skin is rough. None of the above-mentioned prior arts related to hydroxyapatite cosmetics have been satisfactory in improving the modulation of epidermal keratin turnover and improving the prevention of skin aging.

[0006]

Means for Solving the Problems Accordingly, the present inventors have:
Cosmetics that act on the epidermal keratinocytes in the skin to promote the metabolism of the cells, restore damage to the skin cells due to external stimuli, and maintain a youthful skin condition With the aim of providing an excellent hair cosmetic product that acts on the hair papilla to activate the cells to prevent hair loss and promote hair growth, as a result of intensive research on the particle form of hydroxyapatite, it was found that amorphous hydroxy The inventors have found that apatite has the above-mentioned effects, and have completed the present invention.

Here, the amorphous hydroxyapatite refers to hydroxyapatite having low crystallinity which does not show a diffraction pattern which is a distinctive characteristic of hydroxyapatite. Hydroxyapatite is Ca
_{Although represented by} a stoichiometric composition of ₁₀ (PO ₄ ) ₆ (OH) ₂ , even if the Ca / P molar ratio does not become 1.67, it shows the properties of hydroxyapatite and has non-stoichiometric characteristics. As described above, the amorphous hydroxyapatite used in the present invention refers to an amorphous or crystalline material obtained by reacting a Ca salt and a phosphate at a Ca / P molar ratio of 1.4 to 1.8. Means lower.

In general, there are various methods for synthesizing hydroxyapatite, such as dry synthesis and wet synthesis, but any one obtained by any of these synthesis methods can be used, and the production process is not limited. For example, in the case of wet synthesis, hydroxyapatite can be obtained by dropping phosphoric acid into a calcium hydroxide suspension so as to have a Ca / P ratio of 1.66. Alternatively, a calcium salt aqueous solution (calcium chloride, calcium acetate, calcium nitrate, calcium lactate, etc.) and a phosphate aqueous solution (ammonium phosphate,
Sodium phosphate, potassium phosphate, etc.) are mixed and stirred at a Ca / P ratio of 1.66 to obtain a precipitate. The precipitate is washed and filtered to obtain hydroxyapatite. Alternatively, hydroxyapatite can be obtained by aging calcium phosphate dihydrate in an alkaline solution, followed by filtration and washing to remove the alkaline component in the solution.

The hydroxyapatite powder synthesized as described above is pulverized so that the average particle size becomes 100 nm or less. The method of atomization treatment is not particularly limited as long as the hydroxyapatite fine particles can have an average particle diameter of 100 nm or less. For example, a sand mill type pulverizer can be used. Can be used an ultra high pressure liquid dispersion type disperser.

When the above-mentioned pulverizer or disperser is used for the pulverization, if necessary, citric acid, sodium citrate, potassium citrate, lactic acid, water glass, silicone oil, lecithin, pectin, gelatin Use dispersants such as, stearic acid, calcium stearate, magnesium stearate, casein, albumin, glucose, polypeptide, microcrystalline cellulose, sucrose fatty acid esters, polyglycerin fatty acid esters, propylene glycol fatty acid esters, saponins and the like. Can be. As another pulverizing method, there is a method in which a voltage is applied to a hydroxyapatite plate in vacuum to release hydroxyapatite fine particles.

As described above, the amorphous hydroxyapatite microparticles of the present invention act on epidermal keratinocytes in the skin to promote metabolism, recover skin cell damage caused by external stimuli, and restore the skin of youthful skin. The state can be maintained. The cosmetic of the present invention contains the above-mentioned amorphous hydroxyapatite fine particles, and the amount of the fine particles varies depending on the type of cosmetic. 10.0% by weight is blended. When the amount is less than the above range, the effect of blending the amorphous hydroxyapatite fine particles is extremely low, and when the amount exceeds the above range, the fine particles are easily condensed, resulting in cosmetics. This is because it becomes difficult to stably mix the compounds.

Since apatite fine particles are easily aggregated, they cannot be sufficiently dispersed even when incorporated into a cosmetic base, and aggregation is likely to occur during storage of the product. In particular, when the blending amount of apatite increases, aggregation of the fine particles easily occurs. In order to suppress such agglomeration of the apatite fine particles, it is preferable to mix an organic acid together with the finely divided apatite.
When apatite fine particles are blended together with an organic acid, the surface of the apatite fine particles is dissolved in cosmetics, and the particles can be present in a state having a smaller particle size than before the blending.

As the organic acid used in the present invention, carboxylic acid is used, and α-hydroxy acid (fruit acid) is particularly preferable. The above-mentioned α-hydroxy acid is a general term for compounds having a carboxyl group —COOH and a hydroxyl group —OH, for example, glycolic acid, glucuronic acid,
Examples include pyruvic acid, methyl pyruvate, ethyl pyruvate, salicylic acid, citric acid, lactic acid, tartaric acid, butyric acid, 2-hydroxybutyric acid, and 3-hydroxybutyric acid. One or more of these are used. The compounding amount of the organic acid is in the range of 1 to 500% by weight based on the amount of apatite, and the pH is preferably adjusted to be in the range of 4.5 to 7.0 and used.

If the pH of the cosmetic falls below 4.5, the dissolution of the incorporated apatite proceeds, and the apatite fine particles disappear, which may impair the ultraviolet protection effect. If the pH is higher than 7.0, the apatite particles blended tend to agglomerate, and the effect of the cosmetic of the present invention may be impaired.

The cosmetic of the present invention contains the above-mentioned fine particles of amorphous hydroxyapatite as an essential component, and other commonly used fats and oils, surfactants, water-soluble polymers, preservatives, Various cosmetic ingredients such as drugs, pigments, fragrances, and humectants are appropriately blended. Examples of the fats and oils include liquid paraffin, petrolatum, paraffin wax, squalene, beeswax, carbana wax, olive oil, lanolin, shellac and the like.

As the above-mentioned surfactant, for example, an anionic surfactant (sodium N-acyl-glutamate,
N-lauroyl-L-glutamic acid-sodium, sodium lauryl sulfate, etc.), nonionic surfactants (such as sorbitan monolaurate, sucrose fatty acid ester, glycerin fatty acid ester, polyoxyethylene cetyl ether), and cationic surfactant (Cetyltrimethylammonium chloride, stearyltrimethylammonium chloride, etc.) and amphoteric surfactants (lauryldimethylaminoacetate betaine, coconut oil fatty acid amidopropyl betaine, etc.).

Examples of the water-soluble polymer include carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, tragacanth gum, carrageenan, dextrin, gelatin, and gum arabic.

Examples of the preservative include sodium benzoate, paraben and the like. Examples of the drug include vitamins, hinokitiol, natural antibacterial agents such as chitosan,
Crude drugs and the like can be mentioned.

The above-mentioned dyes include iron oxide, yellow iron oxide,
Dyes such as titanium oxide, sericite, mica, talc, and chromium oxide are exemplified.

Examples of the flavor include essential oils such as lavender, lemon, lime, jasmine and rose, and animal flavors such as musk.

Examples of the humectant include sorbitol, xylitol, glycerin, maltitol, propylene glycol, 1,3-butylene glycol, polyethylene glycol and the like. The amounts of these commonly used cosmetic ingredients are appropriately adjusted according to the application.

The form of the cosmetic of the present invention is arbitrary and not limited, and examples thereof include liquids, emulsions, creams, gels,
Any material that can be applied to the outer skin such as an aerosol or powder may be used. The types of cosmetics include creams, emulsions, lotions, essences, facial cleansers, basic cosmetics such as packs,
Examples include makeup cosmetics such as foundations and liquid foundations, and toiletry products such as body shampoos, soaps, and deodorants.

Further, the cosmetic of the present invention acts on the hair root and around the hair, and is also effective in protecting the hair. Therefore, the cosmetic is used as a form of a hair product such as a shampoo, a rinse, a treatment, a conditioner, and a hairdressing agent. Can be. Furthermore, when the cosmetic containing the above-mentioned amorphous hydroxyapatite microparticles is directly applied to the skin, it acts on the epidermal keratinocytes in the skin as described above, and has an effect of promoting the metabolism of the cells. The crystalline hydroxyapatite fine particles can also be used as a component of a bath agent.

[0024]

(Example 1) Production of amorphous hydroxyapatite An amorphous hydroxyapatite to be mixed with the cosmetic of the present invention was produced according to the following formulation. First, a 30% by weight aqueous phosphoric acid solution was added dropwise to the stirred calcium hydroxide suspension until the pH of the suspension reached pH 10. The gel-like substance thus formed was aged at room temperature for one day. Thereafter, the gel-like substance was filtered with a glass filter, and the remaining substance was further dried in air at 100 ° C. or higher, to obtain amorphous hydroxyapatite having a Ca / P ratio of 1.62. The average particle size of such amorphous hydroxyapatite is 3.2 μm and the maximum particle size is 21 μm.
Met. Further, the average particle diameter of the amorphous hydroxyapatite powder is 5 using a sand mill (trade name “Dynomill”) manufactured by Shinmaru Enterprises Co., Ltd.
The atomization treatment was performed until the thickness became 0 nm.

FIG. 1 shows a powder X-ray diffraction pattern of the thus obtained amorphous hydroxyapatite fine particles. As can be seen from this diffraction pattern, the amorphous hydroxyapatite has a broad peak unique to the hydroxyapatite crystal, indicating that it is amorphous.

Example 2 Epidermal Cell Proliferation Test An experiment was conducted using a Hartley female guinea pig (body weight: about 300 to 4).
00g) and used in experiments after acclimatizing to the environment for about one week. The experiment was performed in a room controlled at room temperature 24 ° C. and lighting 12L: 12D. To induce division of guinea pig ear skin epidermal cells, the outer ear is gently applied with adhesive tape.
Times stripped. Thereafter, a test sample was applied and processed. The experiment was performed once daily for four consecutive days.

As the test sample, the above-mentioned synthesized amorphous hydroxyapatite (hereinafter, powdered HAP) and apatite obtained by pulverizing the same (hereinafter, referred to as finely divided HAP) were used. Each test sample was dispersed 1% in physiological saline containing 1% DMSO. On the last day, dividing epidermal cells were arrested in metaphase by intraperitoneally administering 2 mg / kg of colcemide dissolved in physiological saline to guinea pigs. Four hours after administration of colcemide, guinea pigs were killed with ether, and ears treated with test samples were collected and fixed with 10% buffered formalin solution. The fixed ears are dehydrated and
After embedding in paraffin and sectioning at a thickness of 4 μm perpendicular to the skin surface, hematoxylin and eosin staining was performed.

Separately, the test sample was applied and treated without stripping to examine whether hydroxyapatite increases cell division without mechanically stimulating and inducing epidermal cells. Experiments were also conducted. At this time, the application of the test sample was performed for 10 days. In each experiment,
A control group to which no test sample was applied or treated was provided, and 10 test specimens in each group were used for the experiment. Observation around the dermis-epidermal boundary of all tissue specimens was made through a CCD camera attached to a microscope. The number of newly dividing cells was obtained by counting metaphase images per 2 mm of the dermis-epidermal boundary projected on the screen. The data between each group is
By performing a test, statistically significant differences were examined.

[Results and Discussion] FIG. 2 shows the number of metaphase images counted from the tissue treated with powdered HAP and micronized HAP after stripping for 4 consecutive days. That is, FIG. 2 shows the number of metaphase images of epidermal cells in guinea pig ear skin obtained by applying and treating a test sample for 4 consecutive days after 15 times of stripping. Means the mean ± S.D. D.
Indicated by *: Significant difference from control group, P <0.05. *
*: Significant difference between SP-1 and TSP-1, P <0.
05. The control group does not apply and treat the test sample,
This is data obtained by performing only stripping. Without stripping, the number of metaphase images is
It was 3.3 ± 1.6 (hereinafter, average ± SD) (see FIG. 3), but the number of epidermal cells newly dividing by stripping was 23.9 ± 5.1 within 4 hours. Increase. In this state, when powder HAP is further applied and treated, 37.
7 ± 4.9 and 60.5 ± 9.
6 and a division image about 2.5 times or more that of the control group were observed. A statistical difference was also observed between powdered HAP and micronized HAP.

FIG. 3 shows data when powdered HAP and micronized HAP were applied and treated without inducing new division of epidermal cells by stripping. That is, FIG. 3 shows the number of metaphase images of epidermal cells in guinea pig ear skin obtained by directly applying and treating a test sample for 10 days without stripping.
Mean ± S. D. Indicated by *: Significant difference from control group
P <0.05. **: significant difference between SP-1 and TSP-1, P <0.05. The test sample was treated for 10 days, unlike the data shown in FIG.
9.6 ± 2.2 for AP, 11.8 ± for micronized HAP
Even with the application and treatment of 1.5 and the test sample alone, the division image of epidermal cells was significantly increased. Also, it was found that the effect was significantly greater when micronized HAP, which was fine particles, was used rather than powdered HAP.

Example 3 Cell Activity Effect Test [Experiment] The cell activity effect of hydroxyapatite fine particles was examined by the MTT method. Normal human epidermal keratinocytes 10,
Medium containing 2,000 cells / ml (Morinaga Institute of Science, H
100ul of FK Cells Kit) is seeded on a 96-well plate. After 24 hours, 0.1% by weight of hydroxyapatite fine particles are added. After 72 hours from the addition, the MTT reagent is added and the cells are cultured for 4 hours. The cultured cells are lysed with DSMO and the absorbance is measured.
Cell activity. The cell activity was measured using the same formulation as above and adding 0.1% by weight of hydroxyapatite microparticles and 0.01% by weight of lactic acid.

For comparison, the cell activity was measured when various components such as hydroxyapatite having an average diameter of 3 μm, highly crystallized hydroxyapatite, lactic acid and collagen were respectively added. Highly crystallized hydroxyapatite is obtained by firing hydroxyapatite at 1000 ° C. The cell activity ratio was determined assuming that the absorbance of cells without drug added was 1.

[Experimental results] Table 1 shows the experimental results. As can be seen from this table, all of the samples to which the fine particles of hydroxyapatite are added have a cell activity ratio of more than 1.0, and thus have an effect of increasing the cell activity.

[0034]

[Table 1]

Example 4 Cell Activity Effect The cell activity effect of the hydroxyapatite fine particles was measured by MTT.
Investigated by the method. Normal human hair nanny 10,000 cells
/ Ml suspended medium (Toyobo / HDPC Tot)
al Kit) was seeded in a 96-well plate.
After 24 hours, 0.1% of hydroxyapatite fine particles
Was added. 72 hours after the addition, the MTT reagent is added and the cells are cultured for 4 hours. The cultured cells were dissolved in DSMO, the absorbance was measured, and the cell proliferation was examined by the absorbance, and the cell activity was determined. The cell activity was measured using the same formulation as above and adding 0.1% by weight of hydroxyapatite fine particles and 0.1% by weight of pentadecanoic acid.

For comparison, hydroxyapatite having an average diameter of 3 μm, highly crystallized hydroxyapatite and pentadecanoic acid were used. Highly crystallized hydroxyapatite is obtained by firing hydroxyapatite at 1000 ° C. Cell proliferation was determined with the absorbance of cells without drug added as 1.

[Experimental results] Table 2 shows the results of the experiment. As can be seen from this table, all of the samples to which the fine particles of hydroxyapatite are added have a cell activity ratio of more than 1.0, and thus have an effect of increasing the cell activity.

[0038]

[Table 2]

(Example 5) Various prescription examples Next, prescription examples in which the above-mentioned amorphous apatite fine particles are blended are shown.

Formulation Example 1 (hair tonic: hair tonic type) (Ingredients) (1) Fine powder of amorphous apatite 1.0 (2) Menthol 0.2 (3) Ethanol 60.0 (4) Purified water Remaining (Production method) ) After dissolving menthol in ethanol, add purified water. Next, amorphous apatite fine particles were added thereto and uniformly stirred to obtain a product.

Formulation Example 2 (Shampoo) (Components) (1) Fine particles of amorphous apatite 1.0 (2) Lactic acid 0.1 (3) Triethanolamine alkyl sulfate 15.0 (4) Coconut fatty acid monoethanolamide 2.0 (5) Ethylene glycol monostearate 5.0 (6) Fragrance 0.5 (7) Purified water residue (Production method) While heating the above components to 86 ° C., uniformly mix and stir, and A shampoo was prepared.

Formulation Example 3 (Rinse) (Components) (1) Fine particles of amorphous apatite 0.2 (2) Stearyl dimethylbenzylammonium chloride 1.4 (3) Stearyl alcohol 0.6 (4) Glycerin monostearate 1 5.5 (5) Propylene glycol 5.0 (6) Purified water residue (Preparation method) The above components were uniformly mixed and stirred while heating to 86 ° C. to prepare a rinse of the present invention.

Formulation Example 4 (Treatment) (Components) (1) Fine particles of amorphous apatite 0.1 (2) Stearyltrimethylammonium chloride 6.0 (3) Polyvinylpyrrolidone 4.0 (4) Propylene glycol 5.0 ( 5) Dye 0.1 (6) Fragrance 0.1 (7) Purified water residue (Preparation method) The above components were uniformly mixed and stirred while heating to 86 ° C. to prepare a treatment of the present invention.

Formulation Example 5 (packing agent) (Component) (1) Fine particles of amorphous apatite 1.0 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) ) Ethanol 10.0 (6) Methylparaben 0.1 (7) Fragrance 0.1 (8) Purified water The remaining (production method) (3), (4) and (6) were added to and dissolved in (8). Next, (2) was added and dissolved while stirring at a temperature of 75 ° C.
Disperse (1). (5) and (7) were added thereto and stirred to obtain a pack.

Formulation Example 6 (creamy foundation) (Components) (1) Amorphous apatite fine particles 0.5 (2) Stearic acid 5.0 (3) Lipophilic glyceryl monostearate 2.5 (4) Cetostearyl Alcohol 1.0 (5) Monostearyl alcohol 1.0 (6) Liquid paraffin 7.0 (7) Isopropyl myristate 8.0 (8) Propylene glycol monolaurate 3.0 (9) Methyl paraben 1.5 (10) Triethanolamine 1.2 (11) Sorbit 3.0 (12) Fragrance 0.1 (13) Purified water Remaining (Production method) The components (2) to (7) were stirred and dissolved while heating to 65 ° C. . On the other hand, the components (8) to (12) were stirred and dissolved while heating to 65 ° C. Mix and stir these melts, (13)
The component and the component (1) were added and mixed and stirred uniformly.

Formulation Example 7 (Lotion) (Components) (1) Fine particles of amorphous apatite 0.1 (2) Glycerin 5.0 (3) Polyethylene glycol 0.2 (4) Surfactant 2.0 (5) ) Ethanol 10.0 (6) Fragrance 0.2 (7) Ethylparaben 0.1 (8) Purified water residue (Preparation method)
It was a lotion.

Formulation Example 8 (Emulsion) (Components) (1) Fine particles of amorphous apatite 0.05 (2) Stearic acid 6.0 (3) Beeswax 2.0 (4) Lanolin 4.0 (5) Monostearin Glycerin acid 3.0 (6) Surfactant 0.5 (7) Fragrance 0.5 (8) Ethylparaben 0.1 (9) Purified water Remaining (Preparation method) The above components can be uniformly stirred at room temperature. Solubilized,
It was a lotion.

Formulation Example 9 (cream) (ingredients) (1) cetostearyl alcohol 3.5 (2) squalane 20.0 (3) beeswax 3.0 (4) lanolin 5.0 (5) ethylparaben 0.3 (6) POE (20 mol added) Sorbitan monooleate 2.0 (7) Stearic acid monoglyceride 2.0 (8) Fragrance 0.1 (9) Fine particles of amorphous apatite 3.0 (10) Citric acid 0 .04 (11) 1,3-butylene glycol 5.0 (12) Glycerin 5.0 (13) Purified water Remaining (Production method) The components of (1) to (8) are uniformly mixed while heating to 75 ° C. Stir and dissolve (oil phase). After dissolving the components of (11) and (12) in (13), add (9) and (10) and disperse
Heat to ° C (aqueous phase). The oil phase was added to the aqueous phase, emulsified by a homomixer treatment, and then cooled to obtain a cream.

Formulation Example 10 (facial cleansing cream) (Ingredients) (1) Potassium N-lauroyl-L-glutamate 10.0 (2) Potassium stearate 5.0 (3) Potassium myristylate 5.0 (4) Amorphous Apatite fine particles 6.0 (5) 1,3-butylene glycol 20.5 (6) Glycerin 5.0 (7) Purified water Remaining (Production method) The above components (1) to (7) are heated to 80 ° C. While stirring and mixing uniformly, a face wash cream was prepared.

Formulation Example 11 (Body Shampoo) (Components) (1) Sodium N-acyl-L-glutamate 10.0 (2) Sodium N-lauroylglycine 10.0 (3) Potassium myristate 5.0 (4) ) Potassium laurate 5.0 (5) Propylene glycol 3.0 (6) Methylparaben 0.3 (7) Fine particles of amorphous apatite 3.0 (8) Purified water Remaining (Preparation method) Heat the above components to 80 ° C While stirring, the mixture was mixed and stirred to prepare a body shampoo.

Formulation Example 12 (bath agent) (Components) (1) Sodium sulfate 29.0 (2) Sodium bicarbonate 28.5 (3) Magnesium sulfate 29.0 (4) Calcium silicate 1.0 (5) Fragrance 1.5 (6) Liquid silicon 1.0 (7) Amorphous apatite fine particles 10.0 (8) Salicylic acid 1.0 (Production method) The above components were uniformly mixed and stirred to prepare a bath agent.

Formulation Example 13 (Soap bar) (Ingredients) (1) Soap base (tallow: coconut oil fatty acid = 8: 2) 41.0 (2) Sodium N-acyl-L-glutamate 5.0 (3) Concentrated glycerin 8.0 (4) tartaric acid 1.0 (5) amorphous apatite fine particles 8.0 (6) edetate 0.1 (7) purified water remaining (production method) The above components were charged into a heating kneader, The mixture was uniformly mixed and stirred while being heated to 80 ° C., and pelletized by a hot roll and a pelletizer to obtain a solid soap.

[Comparative Examples 1 to 13 and Comparative Examples 1 to 13] In place of the amorphous fine particles of apatite used in the above-mentioned Formulation Examples 1 to 13, highly crystallized hydroxyapatite was blended. Comparative Examples 1 to 13 were prepared in the same manner as in Examples 1 to 13. Further, as a control example, purified water was blended in place of the amorphous apatite fine particles used in the above-mentioned formulation examples 1 to 13, and the same method as in formulation examples 1 to 13 was used. Was prepared.

(Example 6) Monitor test 1 Fifty healthy adults aged 22 to 58, randomly selected as subjects, were treated as prescription examples 1-4 and comparative examples 1-4.
After using each of the control examples 1 to 4 for one month, the following judgments were made on (A) stiffness, (B) gloss, and (C) pores of hair. The stain on the pores was observed with a microscope. The contents of the evaluation criteria and the average score of 50 persons are shown below.

[0055]

[Table 3]

[0056]

[Table 4]

From the results in Table 4, it can be seen that Formulation Examples 1 to 4 of the present invention.
The composition of No. 4 enhances the stiffness of the hair, improves the gloss of the hair,
It was found that it was highly effective in removing pore dirt.

(Example 7) Monitor test 2 Prescription examples 5 to 10 and comparative examples 5-1 were randomly selected from 50 healthy adults aged 22 to 58 years old as subjects.
0 and each of Control Examples 5 to 10 were used for one month, and then the following evaluation was performed. (A) Improvement effect on skin dullness and spots: The condition of the skin was visually observed. (B) Effect on fine wrinkles: Skin condition was visually observed. (C) Reduction effect on the variation in the area between the stratum corneum, 30 cells on the left cheek of the subject's face were peeled off using a double-sided paper tape by a tape stripping method, and the area of each cell was measured. The standard deviation was calculated and evaluated for the obtained cell area. The contents of the evaluation criteria and the average score of 50 persons are shown below.

[0059]

[Table 5]

[0060]

[Table 6]

From the results in Table 6, it can be seen from Formulation Examples 5 to 5 according to the present invention.
It can be seen that the composition No. 10 is highly effective in reducing dullness, spots and fine lines on the skin. Thus, it can be seen that the composition of the present invention has a high effect of improving epidermal keratin turnover modulation and an effect of preventing skin aging. In addition, from the results in Table 6, it can be seen that in the case of the compositions of Formulation Examples 5 to 10 of the present invention, the intercellular area is significantly higher than the comparative example and the control example. Since the decrease in the intercellular area is considered to be due to an increase in the number of epidermal cells, it is considered that the composition of the present invention has a high effect of improving the activity of epidermal cells.

Example 8 Monitor Test 3 Prescription Examples 11 to 13 and Comparative Example 11 were randomly selected from 50 healthy adults aged 22 to 58 years old as subjects.
After the use of 13 and control examples 11 to 13 for one month, the following evaluation was performed. In addition, about 40 ° C about bath agent
Was added to and dissolved in 200 L of warm water. (A) Improvement effect on skin dullness and spots: The condition of the skin was visually observed. (B) The effect of reducing the variation in the area between the keratins, the keratinocytes of 30 cells in the left flank of the subject were stripped off using a double-sided paper tape by a tape stripping method, and the area of each cell was measured. The standard deviation was calculated and evaluated for the obtained cell area. The contents of the evaluation criteria and the average score of 50 persons are shown below.

[0063]

[Table 7]

[0064]

[Table 8]

From the results in Table 8, it is found that Formulation Example 11 according to the present invention
It can be seen that compositions Nos. 13 to 13 are high in improving dullness and spots on the skin. Thus, it can be seen that the composition of the present invention has a high effect of improving epidermal keratin turnover modulation and an effect of preventing skin aging. Also, from the results in Table 8,
In the case of the compositions of Formulation Examples 11 to 13 of the present invention, it can be seen that the intercellular area is significantly higher than the comparative example and the control example.
Since the decrease in the intercellular area is considered to be due to an increase in the number of epidermal cells, the composition of the present invention is considered to have a high effect of improving the activity effect of the epidermal cells.

[Brief description of the drawings]

FIG. 1 is a powder X-ray diffraction pattern diagram of amorphous HAP (hydroxyapatite) fine particles.

FIG. 2 shows the number of metaphase images counted from tissue treated with powdered HAP and micronized HAP after 4 consecutive days of stripping.

FIG. 3 is a diagram showing the number of metaphase images without stripping.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A61K 7/00 A61K 7/00 U 7/021 7/021 7/06 7/06 7/075 7/075 7/08 7/08 7/50 7/50 // C11D 3/12 C11D 3/12 3/20 3/20 (72) Inventor Kaichiro Kikukawa 3-11-6 Tsukiji, Chuo-ku, Tokyo Co., Ltd. F-term in Sangi (reference) 4C083 AA082 AB291 AB292 AB312 AB352 AB362 AB382 AC022 AC072 AC102 AC122 AC132 AC231 AC242 AC301 AC302 AC352 AC392 AC402 AC422 AC442 AC472 AC482 AC532 AC542 AC642 AC662 AC692 AC782 AD042 AD072 AD112 AD152 AD512 AD532 CC12 CC0512 CC23 CC25 CC33 CC37 CC38 CC39 DD21 DD22 DD23 DD27 DD31 DD39 EE06 EE07 EE10 EE12 EE28 EE29 FF01 FF05 4H003 AB27 AB46 AC13 BA12 DA02 EA24 EB08 EB09 ED02 FA01 FA02

Claims (6)

[Claims] 1. A cosmetic comprising amorphous hydroxyapatite fine particles. 2. A cosmetic composition comprising amorphous hydroxyapatite fine particles and an organic acid. 3. The cosmetic according to claim 1, wherein the average particle diameter of the amorphous hydroxyapatite fine particles to be blended is 100 nm or less. 4. The cosmetic according to claim 1, wherein the amount of the amorphous hydroxyapatite fine particles is 0.01 to 10% of the total cosmetic composition. 5. The method according to claim 1, which is for hair.
Or the cosmetic according to 2. 6. The organic acid is an α-hydroxy acid,
The cosmetic according to claim 1 or 2, wherein the / P molar ratio is from 1.4 to 1.8.