JP2001186814A - Method for preparing new rooted cutting - Google Patents

Method for preparing new rooted cutting

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Publication number
JP2001186814A
JP2001186814A JP37491399A JP37491399A JP2001186814A JP 2001186814 A JP2001186814 A JP 2001186814A JP 37491399 A JP37491399 A JP 37491399A JP 37491399 A JP37491399 A JP 37491399A JP 2001186814 A JP2001186814 A JP 2001186814A
Authority
JP
Japan
Prior art keywords
cuttings
rooting
liquid medium
cutting
carbon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP37491399A
Other languages
Japanese (ja)
Other versions
JP3861542B2 (en
Inventor
Akira Murakami
章 村上
Toshiaki Tanabe
稔明 田辺
Kunimutsu Murakami
邦睦 村上
Original Assignee
Nippon Paper Industries Co Ltd
日本製紙株式会社
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Filing date
Publication date
Application filed by Nippon Paper Industries Co Ltd, 日本製紙株式会社 filed Critical Nippon Paper Industries Co Ltd
Priority to JP37491399A priority Critical patent/JP3861542B2/en
Publication of JP2001186814A publication Critical patent/JP2001186814A/en
Application granted granted Critical
Publication of JP3861542B2 publication Critical patent/JP3861542B2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • Y02P60/216

Abstract

PROBLEM TO BE SOLVED: To provide a method for preparing new rooted cutting, applicable even to a plant whose growth by cuttage regarded as impossible or difficult, having high productivity. SOLUTION: A rooting bed wetted with a liquid medium containing nitrogen, phosphorus and potassium as essential elements but not a carbon source is prepared in a culture container, a cutting ear is inserted into the rooting bed, cultured and rooting is carried out from the cutting ear while controlling the concentration of carbon dioxide gas in the culture container.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【0001】[0001]

【発明の属する技術分野】本発明は、優良形質を備えた
クローン苗を、挿し木法により、商業的に大量生産する
技術に関する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a technique for commercial mass production of cloned seedlings having excellent traits by a cutting method.

【0002】[0002]

【従来の技術】挿木は、人為的に切断された植物組織
(挿し穂)の切断部位を発根床に挿し入れて、発根床内
で発根させ、独立した一個の植物体を作り出す伝統的な
栄養繁殖方法であり、草本植物から木本植物に到るま
で、親植物と同一の遺伝的性質を備えた個体、即ち、ク
ローン苗を大量に作出・増殖する方法として普及してい
る。
2. Description of the Related Art Cutting is a tradition in which a cut portion of an artificially cut plant tissue (cutting) is inserted into a rooting bed and roots are formed in the rooting bed to produce an independent plant. This is a popular vegetative propagation method, and is widely used as a method for producing and growing a large number of individuals having the same genetic properties as the parent plant, that is, clone seedlings, from herbaceous plants to woody plants.

【0003】この方法は、簡便で、一度に大量の苗を得
るのに適しており、優良形質を備えた個体を低コストで
大量生産できることから、商業的に有利な方法である
が、全ての植物がこの方法で繁殖できるわけではない。
この方法を用いても、挿し穂が全く発根しなかったり、
その発根率が極めて低く、繁殖することができない種や
品種は数多くある。
This method is simple and suitable for obtaining a large number of seedlings at one time, and is capable of mass-producing individuals with excellent traits at low cost. Plants cannot be propagated this way.
Even if this method is used, the cuttings do not root at all,
There are many species and varieties whose rooting rate is extremely low and cannot be propagated.

【0004】また、挿し木は、戸外又は温室・フレーム
等で行なわれる。一方、この挿し木法においては、挿し
穂が発根し、健全な苗を形成するまでの間、その環境を
比較的高湿度に保たなければならない。湿度が低いと、
その葉からの蒸散作用等によって、挿し穂が萎れて弱っ
てしまうからである。しかし、戸外はもちろん、温室や
フレーム内で挿し木を行なう場合でも、高湿度の環境を
維持するには、なかなか手間がかかるのである。
Cutting is performed outdoors or in a greenhouse or frame. On the other hand, in this cutting method, the environment must be maintained at a relatively high humidity until the cuttings start rooting and form healthy seedlings. When the humidity is low,
This is because the cuttings are withered and weakened due to the transpiration from the leaves. However, even when cutting in a greenhouse or frame, as well as outdoors, it takes a lot of time to maintain a high humidity environment.

【0005】このような事情から、優良形質を持ちなが
ら、その個体を商業的に見合うレベルで増殖できない植
物は、いまだに少なくなかった。
[0005] Under such circumstances, there are still many plants that have excellent traits but cannot reproduce the individual at a commercially acceptable level.

【0006】[0006]

【発明が解決しようとする課題】本願発明は、従来、挿
し木による増殖が不可能又は困難とされていた植物にも
適用できる、挿し木苗の作出法を提供することを目的と
する。
SUMMARY OF THE INVENTION An object of the present invention is to provide a method for producing a cutting seedling which can be applied to a plant which has heretofore been impossible or difficult to propagate by cutting.

【0007】また、本願発明は、挿し穂から苗が形成さ
れるまでの間の環境を、高湿度に維持することができ、
苗の生産性を向上させることができる、挿し木苗の作出
法を提供することをも目的とする。
[0007] Further, the present invention can maintain the environment from cutting to the formation of a seedling at high humidity,
It is another object of the present invention to provide a method for producing cuttings that can improve the productivity of seedlings.

【0008】さらに、本願発明は、多くの植物に適用で
きる、商業的に有利なクローン苗の大量増殖方法を提供
することをも目的とする。
Another object of the present invention is to provide a commercially advantageous method for mass-producing cloned seedlings that can be applied to many plants.

【0009】[0009]

【課題を解決するための手段】本願発明者らは鋭意研究
の結果、培養容器内で、植物の生育に必要な栄養素を与
えて挿し穂を培養することにより、上記目的が達成され
ることを見出し、本願発明を完成した。
Means for Solving the Problems As a result of intensive studies, the inventors of the present invention have found that the above object can be achieved by cultivating cuttings by providing nutrients necessary for plant growth in a culture vessel. Heading, the present invention has been completed.

【0010】即ち、本願発明は、窒素、リン、カリウム
を必須元素として含み、かつ、炭素原を含まない液体培
地で湿潤させた発根床を培養容器内に用意し、これに挿
し穂を挿し付けて、培養容器内の炭酸ガス濃度を制御し
つつ、挿し穂からの発根を行なわせることを特徴とする
挿し木苗の作出法を提供するものである。
That is, the present invention provides a rooting bed which contains nitrogen, phosphorus and potassium as essential elements and is moistened with a liquid medium containing no carbon source, in a culture vessel, into which cuttings are inserted. In addition, the present invention provides a method for producing a cutting seedling, wherein rooting is performed from cuttings while controlling the concentration of carbon dioxide in a culture vessel.

【0011】[0011]

【発明の実施の形態】以下、本願発明について更に詳細
に説明する。
BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail.

【0012】本願発明を適用できる植物の種類に特に制
限はない。ユーカリ、アカシア、ヤマモモ、クヌギ、ブ
ドウ、リンゴ、サクラ、バラ、ツバキ、ウメ等の木本植
物の他、キクやカーネーション等の草本植物にも本願発
明を適用することができる。また、従来、挿し木法によ
る繁殖が困難であるとされてきた植物にも、そうでない
ものにも、本願発明を適用することができる。
There is no particular limitation on the types of plants to which the present invention can be applied. The present invention can be applied to herbaceous plants such as chrysanthemums and carnations in addition to woody plants such as eucalyptus, acacia, bayberry, oak, grape, apple, cherry, rose, camellia, and plum. In addition, the present invention can be applied to plants that have conventionally been difficult to reproduce by the cutting method and those that do not.

【0013】挿し穂としては、緑枝(当年枝)や熟枝
(前年以前に伸びた枝)の他、芽や葉も用いることがで
きる。木本植物の場合は緑枝や熟枝、草本植物の場合は
葉や芽を用いるのが普通である。なお、従来の挿し木苗
の作出法においては、挿し穂として枝を用いる場合に
は、その枝についた葉の蒸散作用を抑制するために、葉
の一部を切除する必要があったが、本願発明において、
挿し穂は高湿度下に置かれるので、かかる処理を施さな
くとも、その蒸散作用は抑制される。従って、このよう
な前処理は必要とされない。
As cuttings, in addition to green branches (current branches) and mature branches (branches that have grown before the previous year), shoots and leaves can also be used. In the case of woody plants, it is common to use green branches and mature branches, and in the case of herbaceous plants, leaves and buds are used. In the conventional method for producing cuttings, when using a branch as a cutting, it was necessary to remove a part of the leaf in order to suppress the transpiration of the leaf attached to the branch. In the invention,
Since the cuttings are placed under high humidity, the transpiration is suppressed even without such treatment. Therefore, such pre-processing is not required.

【0014】本願発明の挿し木苗の作出法は、液体培地
で湿潤させた発根床を用い、培養容器内で行なうこと
で、挿し穂及びこれから形成される苗を取り巻く環境の
湿度維持を容易とする。例えば、この培養容器内で作出
される挿し木苗の高さより、開口部が高い位置にある広
口フラスコ等を利用すれば、挿し穂から健全な苗が形成
されるまでの間、培養環境は、比較的高湿度に自然と保
たれる。また、かかる容器ばかりではなく、容器開口部
の大きさや位置を工夫することで、種々の形態の容器に
おいて、培養容器内の環境を高湿度に保つようにするこ
とができる。従って、本願発明においては、作業性等を
考慮し、種々の形態の容器を、その培養容器として選択
することができる。なお、培養容器内の環境を高湿度に
保つという観点から、最も好ましいのは密閉容器の使用
であるが、この場合でも、培養容器内には炭酸ガスが供
給されるようにしなければならない。例えば、容器開口
部を炭酸ガス透過性の膜で蔽う等の方法を、このような
目的のため、採用することができる。
The method for producing cuttings of the present invention uses a rooting bed moistened with a liquid medium and is carried out in a culture vessel, so that it is easy to maintain the humidity of the environment surrounding the cuttings and the seedlings formed therefrom. I do. For example, if a wide-mouthed flask or the like with an opening higher than the height of the cuttings produced in this culture vessel is used, the culture environment can be compared until healthy seedlings are formed from the cuttings. Naturally kept at high humidity. By devising not only such a container but also the size and position of the container opening, the environment in the culture container can be maintained at a high humidity in various types of containers. Therefore, in the present invention, various types of containers can be selected as the culture container in consideration of workability and the like. From the viewpoint of maintaining the environment in the culture vessel at a high humidity, the use of a closed vessel is the most preferable, but even in this case, carbon dioxide must be supplied into the culture vessel. For example, a method of covering the container opening with a carbon dioxide gas permeable membrane can be employed for such a purpose.

【0015】発根床としては、液体培地により実質的に
均一に湿潤されるものであって、かつ、これに挿し付け
られる挿し穂を、その挿し付けた状態で保持できるよう
なものを用いる。例えば、砂、赤玉土等の自然土壌、バ
ーミキュライト、パーライト、ガラスビーズ等の人工土
壌、又は発泡フェノール樹脂、ロックウール等の多孔性
成形品等を培養容器内に入れ、これを発根床として使用
することができる。
The rooting bed is one that is substantially uniformly wetted by the liquid medium and that can hold the cuttings inserted thereinto in the inserted state. For example, sand, natural soil such as akadama, artificial soil such as vermiculite, perlite, glass beads, or a porous molded product such as foamed phenolic resin and rock wool are placed in a culture vessel and used as a rooting bed. can do.

【0016】本願発明において液体培地は、窒素、リ
ン、カリウムを必須元素とする。これら各々の元素の濃
度は、その適用する植物の種類に応じて調整する。この
ような液体培地としては、市販の家庭園芸用複合肥料や
公知の植物組織培養用液体培地をそのまま、又は適宜希
釈して用いることができる。例えば、家庭園芸用複合肥
料としては、窒素、リン、カリウムを主要成分とする
「ハイポネックス液5−10−5(登録商標)」
((株)ハイポネックスジャパン製)液を250〜50
0倍に希釈した溶液が、植物組織培養用液体培地として
は、ムラシゲ・スクーグ培地(Murashige and Skoog(19
62)、以下、MS培地と略記する。)を4〜16倍に希
釈した溶液が、本発明において、汎用性の高い液体培地
として使用できる。
In the present invention, the liquid medium contains nitrogen, phosphorus and potassium as essential elements. The concentration of each of these elements is adjusted according to the type of plant to which the element is applied. As such a liquid medium, a commercially available complex fertilizer for home gardening or a known liquid medium for plant tissue culture can be used as it is or appropriately diluted. For example, as a compound fertilizer for home gardening, "Hyponex liquid 5-10-5 (registered trademark)" containing nitrogen, phosphorus, and potassium as main components is used.
(Manufactured by Hyponex Japan Co., Ltd.)
A 0-fold diluted solution is used as a liquid medium for plant tissue culture as Murashige and Skoog (19).
62), hereinafter abbreviated as MS medium. ) Can be used as a highly versatile liquid medium in the present invention.

【0017】なお、上記MS培地を始め、公知の植物組
織培養用培地は、窒素、リン、カリウムの他、多量元素
として水素、炭素、酸素、硫黄、カルシウム、マグネシ
ウムを、微量元素として鉄、マンガン、銅、亜鉛、モリ
ブデン、ホウ素、塩素を、無機塩類、又は、チアミン、
ピリドキシン、ニコチン酸等のビタミン類として含んで
いる。従って、本願発明の液体培地としては、窒素、リ
ン、カリウムの他、これらの元素を無機塩類又はビタミ
ン類等として含有しているものも、使用することができ
る。
In addition to the above-mentioned MS medium, known mediums for culturing plant tissues include nitrogen, phosphorus, potassium, hydrogen, carbon, oxygen, sulfur, calcium, and magnesium as major elements and iron, manganese as trace elements. , Copper, zinc, molybdenum, boron, chlorine, inorganic salts, or thiamine,
Contains as vitamins such as pyridoxine and nicotinic acid. Therefore, as the liquid medium of the present invention, those containing these elements as inorganic salts or vitamins in addition to nitrogen, phosphorus and potassium can also be used.

【0018】また、本願発明において使用する液体培地
には、更に、植物生長調整物質を添加することもでき
る。例えば、植物組織からの不定根発生を促進する、イ
ンドール酢酸、インドール酪酸(IBA)、ナフタレン
酢酸等のオーキシン類を単独で又は2種以上組合せて、
本願発明の液体培地に0.1〜10mg/l添加するこ
とにより、挿し穂からの発根、即ち挿し木苗の形成を促
進することができる。
The liquid medium used in the present invention may further contain a plant growth regulator. For example, auxins, such as indoleacetic acid, indolebutyric acid (IBA), and naphthaleneacetic acid, which promote adventitious root development from plant tissues, alone or in combination of two or more,
By adding 0.1 to 10 mg / l to the liquid medium of the present invention, rooting from cuttings, that is, formation of cuttings, can be promoted.

【0019】一方、本願発明の液体培地には、ショ糖等
の炭素源は含まれない。炭素源は、多くの生物に共通す
るエネルギー源であるが、本願発明では、通常の挿し木
苗の作出法と同様、無菌条件下での操作を特に必須の条
件とはしていないため、炭素原を含有する培地を用いる
と、挿し穂に付着した雑菌や、培養環境中の雑菌も培地
中の炭素源を栄養源として繁殖し、挿し穂や、これから
形成される苗の枯死をもたらすからである。
On the other hand, the liquid medium of the present invention does not contain a carbon source such as sucrose. The carbon source is an energy source common to many organisms. However, in the present invention, as in the ordinary method for producing cuttings and seedlings, operation under aseptic conditions is not a particularly essential condition. When a medium containing is used, various bacteria attached to the cuttings and various bacteria in the culture environment also propagate using the carbon source in the medium as a nutrient source, causing the cuttings and seedlings formed therefrom to die. .

【0020】もっとも、挿し穂から形成される苗はもち
ろん、挿し穂自体も、自ら光合成を行なう能力を有して
いるので、植物の生育に適当な強度の光を与えることに
より、炭酸ガスを同化してエネルギー源とすることがで
きる。そのため、本願発明の挿し穂においても、栄養素
として炭素源を付与しなくとも、大気中の炭酸ガスを利
用して発根等を行なうことができる筈である。しかし、
本願発明において、窒素、リン、カリウム等の栄養素を
液体培地により与えられた挿し穂は、活発に光合成を行
なうため、この炭酸ガスの濃度を人工的に制御する必要
が生ずる。即ち、挿し穂の活発な光合成により、培養容
器内の炭酸ガス濃度は低下するので、これを、人為的に
補う必要があるのである。さもなければ、たとえ、炭酸
ガス以外の栄養素を、この挿し穂に十分供給したとして
も、やがてその光合成能は低下し、挿し穂からの発根、
つまりは苗の形成が阻害されることとなる。
However, not only the seedlings formed from the cuttings but also the cuttings themselves have the ability to perform photosynthesis by themselves. Therefore, by giving light of an appropriate intensity to the growth of plants, carbon dioxide can be removed. Into an energy source. Therefore, even in the cuttings of the present invention, rooting and the like should be able to be performed using carbon dioxide gas in the atmosphere without providing a carbon source as a nutrient. But,
In the present invention, cuttings to which nutrients such as nitrogen, phosphorus and potassium are given in a liquid medium actively perform photosynthesis, so that it is necessary to artificially control the concentration of this carbon dioxide gas. That is, the active photosynthesis of the cuttings lowers the concentration of carbon dioxide in the culture vessel, and it is necessary to artificially compensate for this. Otherwise, even if nutrients other than carbon dioxide are sufficiently supplied to the cuttings, their photosynthetic ability will eventually decrease and rooting from the cuttings,
That is, seedling formation is inhibited.

【0021】培養容器内の挿し穂に活発に光合成を行な
わせ、その発根率を向上させるため必要とされる炭酸ガ
ス濃度は、挿し穂とする植物の種類によって異なる。し
かし、一般的には、培養容器内の炭酸ガス濃度を300
〜1500ppmに制御するのが好ましい。培養容器内
の炭酸ガス濃度が300ppmより低いと、挿し穂の光
合成能も発根率も、大幅な向上を期待できない。また、
培養容器内の炭酸ガス濃度を1500ppmより高めて
も、挿し穂の光合成能や発根率は、その炭酸ガス濃度に
見合った向上を示さなくなる。炭酸ガス濃度の制御は、
各培養容器ごとに行なってもよいが、培養容器が置かれ
る環境自体の炭酸ガス濃度を制御することで、培養容器
内を所定の炭酸ガス濃度に制御する方が、簡便で、コス
ト的にも有利である。このとき、培養容器は、開口部を
そのまま開放したものや、前記したように、その開口部
を炭酸ガス透過性の膜で覆ったものを使用することがで
きる。
[0021] The carbon dioxide concentration required for vigorously photosynthesizing the cuttings in the culture vessel and improving the rooting rate varies depending on the type of plant used as the cuttings. However, generally, the concentration of carbon dioxide in the culture vessel is set to 300.
It is preferable to control to 5001500 ppm. If the concentration of carbon dioxide in the culture vessel is lower than 300 ppm, neither photosynthetic ability nor rooting rate of cuttings can be expected to be significantly improved. Also,
Even if the concentration of carbon dioxide in the culture vessel is increased to more than 1500 ppm, the photosynthetic ability and rooting rate of the cuttings will not show an improvement corresponding to the carbon dioxide concentration. Control of carbon dioxide concentration
Although it may be carried out for each culture vessel, it is simpler and more cost effective to control the inside of the culture vessel to a predetermined carbon dioxide concentration by controlling the carbon dioxide concentration of the environment in which the culture vessel is placed. It is advantageous. At this time, the culture vessel may be one having the opening portion opened as it is or one having the opening portion covered with a carbon dioxide permeable membrane as described above.

【0022】本願発明の挿し木苗の作出法においては、
他の条件、即ち、挿し穂を培養するにあたっての温度や
光強度の条件に特に制限はない。その挿し穂の由来する
植物が、光合成をするのに適した条件を適宜採用すれば
よい。一般的には、温度20〜30℃、光強度40〜1
00μmol/m2/sec程度の条件が、この目的の
ために採用される。また、本願発明においては、光を照
射して培養を行なう明期と、暗黒下で培養を行なう暗期
とを設定し、この明期・暗期を交互に繰返して培養を行
なってもよい。この場合、光合成は明期においてのみ行
なわれるので、培養容器内の炭酸ガス制御も、明期にお
いてのみ行えばよい。
In the method for producing cuttings of the present invention,
There is no particular limitation on other conditions, that is, the conditions of temperature and light intensity for culturing the cuttings. Conditions suitable for the plant from which the cuttings are derived to perform photosynthesis may be appropriately adopted. Generally, a temperature of 20 to 30 ° C. and a light intensity of 40 to 1
Conditions on the order of 00 μmol / m 2 / sec are employed for this purpose. In the present invention, a light period in which the culture is performed by irradiating light and a dark period in which the culture is performed in the dark may be set, and the culture may be performed by alternately repeating the light period and the dark period. In this case, since photosynthesis is performed only during the light period, the control of carbon dioxide in the culture vessel may be performed only during the light period.

【0023】なお、前記したように、本願発明において
は、特に無菌下で作業を行なう必要はない。しかし、よ
り健全な苗の作出のため万全を期すには、培養容器、液
体培地、発根床については、挿し穂の挿し付け前に、予
め感熱滅菌やオートクレーブ滅菌等の処置を行なってお
くことが好ましい。
As described above, in the present invention, it is not necessary to perform the operation under aseptic conditions. However, in order to ensure a healthy seedling, the culture container, liquid medium, and rooting bed must be subjected to heat sterilization, autoclave sterilization, etc. before inserting cuttings. Is preferred.

【0024】本願発明において作出された挿し木苗は、
発根後、直ちに培養容器から取出して育苗容器に移植
し、育成することができる。育苗容器に移植する際の用
土や、苗を育成する際の温度・光強度等の条件は、その
植物に適するように適宜設定すればよい。かかる育成過
程を経ることによって、植林等、所定の目的に使用可能
な苗とすることができる。
The cuttings produced in the present invention are:
Immediately after rooting, it can be taken out of the culture container, transplanted into a seedling raising container, and grown. Conditions such as soil used for transplanting to the seedling raising container and temperature and light intensity for growing seedlings may be appropriately set so as to be suitable for the plant. Through such a breeding process, a seedling that can be used for a predetermined purpose such as tree planting can be obtained.

【0025】[0025]

【作用】従来、挿し木によって苗を作出・増殖する場合
には、挿し穂の発根前において養分を与えることは禁忌
とされていた。これは、発根前の挿し穂は養分を吸収す
る正常な能力がないため、この段階における養分の付与
は、挿し穂の腐敗を招くだけと考えられていたからであ
る(例えば、『接木、挿し木、取り木の実際』(株)泰
光堂、昭和61年7月1日発行、第34頁)。
In the past, it has been contraindicated to provide nutrients before rooting of cuttings when producing and growing seedlings with cuttings. This is because the pre-root cuttings do not have the normal ability to absorb nutrients, and the application of nutrients at this stage was thought to only cause rot of the cuttings (eg, "grafting, cuttings, The Reality of Toriki ”(Taikodo Co., Ltd., issued on July 1, 1986, p. 34).

【0026】しかし、本願発明者らは、発根前の挿し穂
であっても、窒素、リン、カリウム等の元素を利用する
ことができ、雑菌の繁殖さえ防げれば、これらの元素を
養分として付与することにより、その発根率を飛躍的に
上昇させることを見出した。ここで、雑菌繁殖の原因と
なるのが炭素源である。そこで、本願発明においては、
窒素、リン、カリウムを必須元素として含み、かつ、炭
素源を含まない液体培地を用いて、挿し穂を培養する。
However, the present inventors can utilize elements such as nitrogen, phosphorus, and potassium even in cuttings before rooting, and if these can prevent the propagation of various bacteria, they can nourish these elements. It has been found that the rooting rate is drastically increased by giving the root. Here, it is the carbon source that causes the propagation of various bacteria. Therefore, in the present invention,
The cuttings are cultured using a liquid medium containing nitrogen, phosphorus, and potassium as essential elements and no carbon source.

【0027】一方、植物において、炭素源に代わるエネ
ルギー源として必要とされるのが、大気中に存在する炭
酸ガスである。本願発明の場合、人工的に、培養環境中
のその濃度を制御することにより、上記培地で培養する
挿し穂を発根させて、苗を作出する。大気中に存在する
炭酸ガスの利用だけでは、挿し穂からの発根に不十分だ
からである。
On the other hand, in plants, carbon dioxide present in the atmosphere is required as an energy source instead of a carbon source. In the case of the present invention, the seedlings are produced by artificially controlling the concentration in the culture environment so that the cuttings cultivated in the above medium are rooted. This is because the use of carbon dioxide in the atmosphere alone is not sufficient for rooting from cuttings.

【0028】また、挿し木法により苗を作出しようとす
る場合、挿し穂は、健全な苗が形成されるまでの間、高
湿度下に置かれることが望ましい。しかし、従来、挿し
木は戸外又は温室・フレーム等で行なわれていた。その
ため、挿し穂及びこれから形成される苗の培養環境を高
湿度に維持するには、かなりの手間をかけなければなら
なかった。しかし、本願発明は、液体培地で湿潤させた
発根床を用い、培養容器内で、挿し木苗を作出する。こ
れにより、挿し穂等の培養環境中の湿度を、労せずして
高いレベルに保つことができ、健全な挿し木苗を容易に
作出することができるのである。
When seedlings are to be produced by the cutting method, it is desirable that cuttings be kept at high humidity until healthy seedlings are formed. However, cutting has conventionally been performed outdoors or in a greenhouse or frame. Therefore, in order to maintain the culture environment of the cuttings and the seedlings formed from the cuttings at a high humidity, considerable effort must be taken. However, in the present invention, cuttings are produced in a culture vessel using a rooting bed moistened with a liquid medium. As a result, the humidity in the culture environment such as cuttings can be kept at a high level without any effort, and healthy cuttings can be easily produced.

【0029】[0029]

【実施例】以下に、本発明を実施例に基づいて説明す
る。
DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below based on embodiments.

【0030】[実施例1]40年生のソメイヨシノの当
年枝より、挿し穂となる枝を採取した。この枝を、芽を
一つだけ残して3cm長さにカッティングし、更に、そ
こについていた葉の3分の1にあたる数の葉を切除し
て、挿し穂を調整した。
Example 1 From the current year branch of a 40-year-old Yoshino cherry tree, a branch serving as a cutting was collected. The branches were cut to a length of 3 cm, leaving only one bud, and one third of the leaves were cut off to adjust the cuttings.

【0031】一方、培養容器としては、10cm四方の
ポリカーボネート製容器の上部2ヶ所に直径1cmの穴
を開け、この開口部を炭酸ガス透過性の膜(日本ミリポ
ア(株)製『ミリシール』)で蔽ったものを用意した。
発根床としては、IBA2mg/lをそれぞれ添加し
た、8倍希釈MS培地100ml又は500倍希釈「ハ
イポネックス液5−10−5(登録商標)」100ml
により湿潤させた、発泡フェノール樹脂成形品(日本曹
達(株)製『オアシス』)を使用した。
On the other hand, as a culture vessel, holes having a diameter of 1 cm were formed in two upper portions of a 10 cm square polycarbonate container, and the opening was formed with a carbon dioxide permeable membrane ("Milliseal" manufactured by Nippon Millipore Co., Ltd.). A covered one was prepared.
As a rooting bed, 100 ml of 8-fold diluted MS medium or 100 ml of 500-fold diluted "Hyponex solution 5-10-5 (registered trademark)" added with 2 mg / l of IBA, respectively.
A foamed phenolic resin molded product (“Oasis” manufactured by Nippon Soda Co., Ltd.) moistened by the above method was used.

【0032】挿し木苗の作出は、上記のようにして調整
した挿し穂を、この発根床に、培養容器あたり25本と
なるように挿し付け、各培養容器内の炭酸ガス濃度が、
明期のみ350、500、1000又は1500ppm
となるように制御し、温度22〜24℃、光強度50μ
mol/m2/sec、明期16時間、暗期8時間で培
養することにより行った。なお、培養容器内の炭酸ガス
濃度の制御は、この培養容器が置かれた環境中の炭酸ガ
ス濃度を制御することにより行った。
For the production of cuttings, cuttings prepared as described above were inserted into the rooting bed at a rate of 25 per culture vessel, and the concentration of carbon dioxide in each culture vessel was reduced.
350, 500, 1000 or 1500 ppm only during the light period
At a temperature of 22 to 24 ° C. and a light intensity of 50 μm.
It was performed by culturing at mol / m 2 / sec for 16 hours in the light period and 8 hours in the dark period. The concentration of carbon dioxide in the culture vessel was controlled by controlling the concentration of carbon dioxide in the environment where the culture vessel was placed.

【0033】3週間後、各試験区あたり100本の挿し
穂について、発根の有無を調査した結果を表1に示す。
Three weeks later, Table 1 shows the results of investigation on the presence or absence of rooting for 100 cuttings per test plot.

【0034】[比較例1]発根床を湿潤させる液体培地
として、IBA2mg/lを添加した水を用いた以外
は、実施例1と同様にして、ソメイヨシノより採取・調
整した挿し穂の培養を行い、挿し木苗の作出を試みた。
なお、本実験は、培養容器内の炭酸ガス濃度を1000
ppm(明期のみ)に制御した場合のみについて行っ
た。
[Comparative Example 1] Culture of cuttings collected and adjusted from Yoshino cherry was carried out in the same manner as in Example 1 except that water containing 2 mg / l of IBA was used as a liquid medium for moistening the rooting bed. We tried to produce cuttings.
In this experiment, the concentration of carbon dioxide in the culture vessel was set to 1000.
This was performed only when the control was performed at ppm (only during the light period).

【0035】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表1に示す。
Three weeks after the cuttings were inserted into the rooting bed, 100 cuttings were checked for rooting, and the results are shown in Table 1.

【0036】[比較例2]培養容器内の炭酸ガス濃度を
全く制御しないで行った以外は、実施例1と同様にし
て、ソメイヨシノより採取・調整した挿し穂の培養を行
い、挿し木苗の作出を試みた。なお、本実験は、発根床
を湿潤させる液体培地として、IBA2mg/l添加の
8倍希釈MS培地を用いた場合のみについて行った。
[Comparative Example 2] In the same manner as in Example 1, except that the carbon dioxide concentration in the culture vessel was not controlled at all, the cuttings collected and adjusted from Yoshino cherry were cultured to produce cuttings. Tried. Note that this experiment was performed only when an 8-fold diluted MS medium containing 2 mg / l of IBA was used as a liquid medium for moistening the rooting bed.

【0037】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表1に示す。なお、このとき、培養容器内の炭酸
ガス濃度は、培養開始直後の明期終了時点までに、既に
100ppm以下となっていた。
Three weeks after the cuttings were inserted into the rooting bed, 100 cuttings were examined for the presence or absence of rooting. Table 1 shows the results. At this time, the concentration of carbon dioxide in the culture vessel had already been 100 ppm or less by the end of the light period immediately after the start of the culture.

【0038】[比較例3]従来法により、挿し木苗の作
出を試みた。
Comparative Example 3 Production of cuttings was attempted by a conventional method.

【0039】実施例1と同様にして採取・調整したソメ
イヨシノの挿し穂の基部に、IBA1重量%を混合した
タルク粉末を塗布し、これを水道水で湿潤させた、実施
例1と同様の発泡フェノール樹脂成形品に挿し付けた。
寒冷紗によって70%遮光された温室内にこのフェノー
ル樹脂成形品をそのまま置いて、挿し穂の培養を行っ
た。
A talc powder mixed with 1% by weight of IBA was applied to the base of a cutting of Yoshino cherry which was collected and adjusted in the same manner as in Example 1, and this was wetted with tap water. It was inserted into a phenolic resin molded product.
The phenolic resin molded product was left as it was in a greenhouse shaded by cold gauze 70%, and the cuttings were cultured.

【0040】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表1に示す。
Three weeks after the cuttings were inserted into the rooting bed, the results of an investigation on the presence or absence of rooting for 100 cuttings are shown in Table 1.

【0041】[0041]

【表1】 表1より明らかなように、培養容器内の炭酸ガス濃度を
350ppm以上に制御し、植物組織培養用培地又は植
物用肥料を液体培地として付与した試験区においては、
液体培地の代わりに水を付与したもの、炭酸ガス濃度の
制御を行わなかったもの又は従来の挿し木法によるもの
と比べて、いずれも、極めて高い挿し穂からの発根率を
示した。
[Table 1] As is clear from Table 1, in the test plot in which the concentration of carbon dioxide in the culture vessel was controlled to 350 ppm or more, and a medium for plant tissue culture or a fertilizer for plant was applied as a liquid medium,
In all cases, the rooting rate from the cuttings was extremely high as compared with those obtained by adding water instead of the liquid medium, those without controlling the concentration of carbon dioxide, and those obtained by the conventional cutting method.

【0042】また、最も高い発根率は、液体培地として
植物組織培養用培地を用いた場合には培養容器内の炭酸
ガス濃度を500ppmに制御した試験区で、一方、液
体培地として植物用肥料を用いた場合には培養容器内の
炭酸ガス濃度を1000ppmに制御した試験区で達成
された。
The highest rooting rate is obtained in a test plot in which the concentration of carbon dioxide in the culture vessel is controlled to 500 ppm when a medium for plant tissue culture is used as the liquid medium, while the fertilizer for plants is used as the liquid medium. Was achieved in a test plot in which the concentration of carbon dioxide in the culture vessel was controlled at 1000 ppm.

【0043】[実施例2]1年生ユーカリプタス・グラ
ブラス(Eucalyptus globulus、以下、E.グロブラス
と略記する。)の頂芽を切除することにより腋芽から枝
を伸長させ、3週間後、この枝の頂端から2節目までを
切取って挿し穂とした。
Example 2 Branches are extended from axillary buds by excision of apical buds of an annual Eucalyptus globulus (hereinafter abbreviated as E. globulus), and after 3 weeks, apical ends of the branches From the first to second joints were cut into cuttings.

【0044】この挿し穂を、実施例1と同様にして培養
し、挿し木苗の作出を行った。但し、このとき、培養容
器内の炭酸ガス濃度を350ppm又は500ppmに
制御した試験区においては、植物組織培養用培地とし
て、8倍希釈MS培地の他に、4倍希釈したMS培地も
使用して挿し木苗の作出を行った(いずれも、IBA2
mg/lを添加。)。
The cuttings were cultured in the same manner as in Example 1 to produce cuttings. However, at this time, in the test plot in which the carbon dioxide concentration in the culture vessel was controlled to 350 ppm or 500 ppm, in addition to the 8-fold diluted MS medium, a 4-fold diluted MS medium was also used as a plant tissue culture medium. Cutting seedlings were produced (all were IBA2
Add mg / l. ).

【0045】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表2に示す。
Three weeks after the cuttings were inserted into the rooting bed, the results of an investigation on the presence or absence of rooting of 100 cuttings are shown in Table 2.

【0046】[比較例4]発根床を湿潤させる液体培地
として、IBA2mg/lを添加した水を用いた以外
は、実施例2と同様にして、E.グロブラスより採取し
た挿し穂の培養を行い、挿し木苗の作出を試みた。な
お、本実験は、培養容器内の炭酸ガス濃度を500pp
m(明期のみ)に制御した場合のみについて行った。
Comparative Example 4 E. coli was used in the same manner as in Example 2 except that water containing 2 mg / l of IBA was used as a liquid medium for moistening the rooting bed. The cuttings collected from Globras were cultured to try to produce cuttings. In this experiment, the concentration of carbon dioxide in the culture vessel was set to 500 pp.
m (only during the light period).

【0047】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表2に示す。
Three weeks after the cuttings were inserted into the rooting bed, Table 2 shows the results of investigation on the presence or absence of rooting for 100 cuttings.

【0048】[比較例5]培養容器内の炭酸ガス濃度を
全く制御しないで行った以外は、実施例2と同様にし
て、E.グロブラスより採取した挿し穂の培養を行い、
挿し木苗の作出を試みた。なお、本実験は、発根床を湿
潤させる液体培地として、IBA2mg/l添加の8倍
希釈MS培地を用いた場合のみについて行った。
Comparative Example 5 E. coli was prepared in the same manner as in Example 2 except that the concentration of carbon dioxide in the culture vessel was not controlled at all. Culture the cuttings collected from Globras,
We tried to create cuttings. Note that this experiment was performed only when an 8-fold diluted MS medium containing 2 mg / l of IBA was used as a liquid medium for moistening the rooting bed.

【0049】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表2に示す。
Three weeks after the cuttings were inserted into the rooting bed, the results of investigation on the presence or absence of rooting of 100 cuttings are shown in Table 2.

【0050】[比較例6]従来法により、挿し木苗の作
出を試みた。
[Comparative Example 6] Production of cuttings was attempted by a conventional method.

【0051】実施例2と同様にして採取したE.グロブ
ラスの挿し穂の基部に、IBA1重量%を混合したタル
ク粉末を塗布し、これを水道水で湿潤させた、実施例2
と同様の発泡フェノール樹脂成形品に挿し付けた。寒冷
紗によって70%遮光された温室内にこのフェノール樹
脂成形品をそのまま置いて、挿し穂の培養を行った。
E. coli collected in the same manner as in Example 2. Example 2 A talc powder mixed with 1% by weight of IBA was applied to the base of a Globras cutting and wetted with tap water.
And inserted into the same foamed phenolic resin molded product as in The phenolic resin molded product was left as it was in a greenhouse shaded by cold gauze 70%, and the cuttings were cultured.

【0052】発根床への挿し穂の挿し付けから3週間
後、100本の挿し穂について、発根の有無を調査した
結果を表2に示す。
Three weeks after the cuttings were inserted into the rooting bed, the results of investigation on the presence or absence of rooting of 100 cuttings are shown in Table 2.

【0053】[0053]

【表2】 表2より明らかなように、培養容器内の炭酸ガス濃度を
350ppm以上に制御し、植物組織培養用培地又は植
物用肥料を液体培地として付与した試験区においては、
液体培地の代わりに水を付与したもの、炭酸ガス濃度の
制御を行わなかったもの又は従来の挿し木法によるもの
と比べて、いずれも、極めて高い挿し穂からの発根率を
示した。即ち、液体培地として植物組織培養用培地又は
植物用肥料のいずれを用いた場合でも、培養容器内の炭
酸ガス濃度を350ppm以上に制御した場合には、ほ
ぼ90%以上の挿し穂から発根が観察された。
[Table 2] As is clear from Table 2, in the test plot in which the concentration of carbon dioxide in the culture vessel was controlled to 350 ppm or more, and a plant tissue culture medium or a plant fertilizer was applied as a liquid medium,
In all cases, the rooting rate from the cuttings was extremely high as compared with those obtained by adding water instead of the liquid medium, those without controlling the concentration of carbon dioxide, and those obtained by the conventional cutting method. That is, no matter whether a plant tissue culture medium or a plant fertilizer is used as the liquid medium, rooting from cuttings of almost 90% or more is obtained when the concentration of carbon dioxide in the culture vessel is controlled to 350 ppm or more. Was observed.

【0054】[0054]

【発明の効果】本願発明によれば、従来の挿し木法によ
っては、挿し穂からの発根が困難であった植物において
も、その発根率が大幅に向上する。
According to the present invention, the rooting rate of a plant that has been difficult to root from cuttings by the conventional cutting method is greatly improved.

【0055】また、本願発明によれば、挿し穂から苗が
形成されるまでの間の環境を、容易に高湿度に維持する
ことができる。
Further, according to the present invention, the environment from cutting to the formation of seedlings can be easily maintained at high humidity.

【0056】従って、本願発明においては、従来法によ
る挿し木苗の作出が困難であった植物からの挿し木苗の
作出が可能となる。また、従来法により、比較的容易に
挿し木苗が作出できた植物にあっては、その生産性を向
上させることができる。しかも、そのための操作は、従
来の挿し木苗の作出法による操作の利点を引継いでお
り、簡便である。
Therefore, according to the present invention, it is possible to produce a cutting seedling from a plant which has been difficult to produce a cutting seedling by the conventional method. In addition, the productivity of a plant from which cuttings and seedlings can be produced relatively easily by the conventional method can be improved. In addition, the operation for this is simple and easy because it inherits the advantages of the conventional cutting method.

【0057】加えて、本願発明においては、培養容器内
で挿し木苗の形成を行うため、湿度のみならず、温度、
光等の条件の制御が、従来法による挿し木苗の作出法と
比べて容易である。
In addition, in the present invention, since cuttings are formed in a culture vessel, not only humidity but also temperature,
Control of conditions such as light is easier than the conventional method for producing cuttings.

【0058】従って、本願発明によれば、挿し木苗の周
年生産を容易に行うことができる。
Therefore, according to the invention of the present application, it is possible to easily carry out year-round production of cuttings.

【0059】即ち、本願発明は、商業的に有利なクロー
ン苗の大量増殖を可能とするものである。
That is, the present invention makes it possible to mass-produce cloned seedlings which are commercially advantageous.

───────────────────────────────────────────────────── フロントページの続き (72)発明者 村上 邦睦 山口県岩国市飯田町2丁目8番1号 日本 製紙株式会社岩国技術研究所内 Fターム(参考) 2B022 AA01 AB20 DA15 EA01 2B030 AA03 AB03 CD03 CD06 CD09 CD14 CD28  ────────────────────────────────────────────────── ─── Continuing from the front page (72) Inventor Kunimitsu Murakami 2-8-1 Iida-cho, Iwakuni-shi, Yamaguchi Japan F-term in Iwakuni Technical Research Laboratory (Reference) 2B022 AA01 AB20 DA15 EA01 2B030 AA03 AB03 CD03 CD06 CD09 CD14 CD28

Claims (7)

【特許請求の範囲】[Claims] 【請求項1】 窒素、リン、カリウムを必須元素として
含み、かつ、炭素原を含まない液体培地で湿潤させた発
根床を培養容器内に用意し、これに挿し穂を挿し付けて
培養し、培養容器内の炭酸ガス濃度を制御しつつ、挿し
穂からの発根を行なわせることを特徴とする挿し木苗の
作出法。
1. A rooting bed containing nitrogen, phosphorus, and potassium as essential elements and moistened with a liquid medium containing no carbon source is prepared in a culture vessel, into which cuttings are inserted and cultured. A method for producing a cutting seedling, wherein rooting is performed from a cutting while controlling the concentration of carbon dioxide in a culture vessel.
【請求項2】 発根床を湿潤させる液体培地として、公
知の植物用肥料をそのまま又は希釈して用いることを特
徴とする、請求項1記載の挿し木苗の作出法。
2. The method for producing cuttings according to claim 1, wherein a known plant fertilizer is used as it is or diluted as a liquid medium for moistening the rooting bed.
【請求項3】 発根床を湿潤させる液体培地として、公
知の植物組織培養用培地をそのまま又は希釈して用い
る、請求項1記載の挿し木苗の作出法。
3. The method for producing cuttings according to claim 1, wherein a known culture medium for plant tissue culture is used as it is or diluted as a liquid medium for moistening the rooting bed.
【請求項4】 発根床を湿潤させる液体培地として、植
物生長調整物質を添加したものを用いる、請求項1、2
又は3に記載の挿し木苗の作出法。
4. A liquid medium to which a plant growth regulator is added is used as a liquid medium for moistening a rooting bed.
Or the method for producing cuttings according to 3 above.
【請求項5】 植物生長調整物質として、オーキシンを
用いる、請求項4記載の挿し木苗の作出法。
5. The method for producing cuttings according to claim 4, wherein auxin is used as a plant growth regulator.
【請求項6】 培養容器内の炭酸ガス濃度を300〜1
500ppmに制御して行う、請求項1、2、3又は4
に記載の挿し木苗の作出法。
6. The concentration of carbon dioxide in the culture vessel is 300 to 1
5. The method according to claim 1, wherein the control is performed at 500 ppm.
Method for producing cuttings described in 1.
【請求項7】 挿し穂として、木本植物由来のものを用
いる、請求項1、2、3、5又は6に記載の挿し木苗の
作出法。
7. The method for producing cuttings according to claim 1, 2, 3, 5, or 6, wherein the cuttings are derived from a woody plant.
JP37491399A 1999-12-28 1999-12-28 How to make new cuttings Expired - Fee Related JP3861542B2 (en)

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JP37491399A JP3861542B2 (en) 1999-12-28 1999-12-28 How to make new cuttings
AU72547/00A AU781465B2 (en) 1999-12-28 2000-12-28 Method for producing rooted cutting

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