JP2001172126A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic giving moderate moistness and wetness without using an adjuvant. SOLUTION: The objective cosmetic contains a hyaluronic acid (salt) having an average molecular weight falling within a specific range or a hyaluronic acid (salt) having an aqueous solution viscosity falling within a specific range.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a cosmetic comprising one or more selected from hyaluronic acid and salts thereof (hereinafter referred to as "hyaluronic acid (salt)").
More specifically, the present invention relates to a cosmetic containing hyaluronic acid (salt) having an average molecular weight within a specific range, and hyaluronic acid (salt) having a specific range of viscosity in an aqueous solution.

[0002]

2. Description of the Related Art Since cosmetics are usually applied directly to the skin, there are some differences in the purpose of use,
It is desired to maintain a moist feeling and a moist feeling after application. N-acetyl-D-glucosamine and D
-Hyaluronic acid (salt), which is a kind of linear acidic mucopolysaccharide composed of glucuronic acid, is used as a kind of cosmetic raw material for obtaining a moist feeling and a moist feeling because of its high water retention. ing.

However, since it is difficult to achieve a moist feeling and a moist feeling with hyaluronic acid (salt) alone, it has been used in combination with an auxiliary agent such as a polyol to obtain a higher effect.

[0004]

However, some of the above-mentioned adjuvants have an unpleasant odor of themselves, and others cause dermatitis or the like in some people. In addition, among the above-mentioned adjuvants, hyaluronic acid (salt)
In some cases, hyaluronic acid (salt) can be decomposed or denatured when used in combination with, and the development of hyaluronic acid (salt) that can maintain a sufficient moist feeling and moist feeling without using an auxiliary agent is expected. Was rare.

[0005]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies in view of the above-mentioned problems of the prior art, and as a result, have found that the viscosity of an aqueous solution of hyaluronic acid (salt) having a specific average molecular weight in a specific range or an aqueous solution thereof. If the cosmetic contains hyaluronic acid (salt) within a specific range, a moderate moist feeling and moist feeling can be obtained without the use of an auxiliary agent, and when the aforementioned auxiliary agent is used. Even so, it was found that the addition amount was relatively small, and the present invention was completed based on this finding.

The present invention comprises the following (1) to (6). (1) The average molecular weight determined by the following formula is 60 to 10.
A cosmetic comprising at least one selected from hyaluronic acid and salts thereof in the range of 10,000. [Η]: Intrinsic viscosity (dl / g) M: Average molecular weight (× 10 ⁴ ) (2) Contains at least one selected from hyaluronic acid having an average molecular weight in the range of 700 to 950,000 and salts thereof. The cosmetic according to claim 1, wherein the cosmetic comprises: (3) The cosmetic according to the above item 1, comprising at least one selected from hyaluronic acid having an average molecular weight in the range of 800,000 to 900,000 and salts thereof. (4) A cosmetic comprising one or more selected from hyaluronic acid and a salt thereof having a viscosity in an aqueous solution of 50 to 100 CS. (5) The content of at least one selected from hyaluronic acid and salts thereof according to any one of the above items 1 to 4, wherein the content is 0.05 to 0.2% by weight relative to the cosmetic. Cosmetics. (6) The composition according to any one of (1) to (4) above, wherein the content of at least one selected from hyaluronic acid and salts thereof is 0.1 to 0.15% by weight based on the weight of the cosmetic. Cosmetics.

Hereinafter, the present invention will be described in detail. In general, hyaluronic acid (salt) is widely distributed in animal tissues, especially in mesenchymal tissues, and contains vitreous, umbilical cord, synovial fluid, membrane fluid, skin,
It is abundantly contained in chicken cockscomb and the like, and can be obtained by extracting from these tissues. Moreover, it can also be obtained by a fermentation method using a hyaluronic acid-producing bacterium of the genus Streptococcus. In the present invention, hyaluronic acid obtained by any method can be used.

[0008] In the case of fermentation, Lancefield
group A and C belonging to the genus Streptococcus (S
Examples of hyaluronic acid-producing bacteria of the genus Streptococcus) include, for example, Streptococcus zooepidemicus.
s), Streptococcus pyogenes (Strept
ococcus py-ogenes), Streptococcus equi
i), Streptococcus equisimilis (Stre
ptococcus equisimilis, Streptococcus dicegalactia (Streptto)
cocc-s dysgalactiae).

The hyaluronic acid salt can be obtained by an alcohol precipitation method in the presence of various salts, and specific examples include a sodium salt and the like.

A first aspect of the present invention is a cosmetic comprising one or more selected from hyaluronic acid and salts thereof having an average molecular weight in the range of 600 to 1,000,000 as determined by the following formula. The average molecular weight of the hyaluronic acid (salt) used in the present invention is preferably from 600 to 1,000,000, more preferably 70 to 1,000,000.
９５950,000, more preferably 800,000 to 900,000.

The average molecular weight is a value obtained by measuring the intrinsic viscosity by a pharmacopoeial viscosity measurement method or the first method of measuring the standard viscosity of a raw material for cosmetics, and using the numerical value to calculate the value from the following equation. [Η]: Intrinsic viscosity (dl / g) M: Average molecular weight (× 10 ⁴ )

The average molecular weight is determined by using sodium hyaluronate having a known molecular weight as a standard substance, which is obtained by the above-mentioned pharmacopoeial viscosity measurement method or cosmetic raw material standard viscosity measurement method.
If a calibration curve prepared using the standard substance is used, it can also be determined by high performance liquid chromatography (hereinafter referred to as “HPCL”).

At that time, HPLC was carried out using Shodex as a column.
It is preferable to use Ionpak KS806 or Ionpak KS-G, and it is preferable to use a 0.2 mol / L aqueous sodium chloride solution as an eluent, flow at a flow rate of 1.0 mL / min, and perform detection at 206 nm.

By using this HPLC, hyaluronic acid (salt) contained in cosmetics can also be analyzed. Even when the peak is not clear due to contamination with impurities, the elution position can be determined by coloring the separated solution by the carbazole sulfate method. And the molecular weight can be measured.

According to a second aspect of the present invention, an aqueous solution having a viscosity of 50
A cosmetic comprising at least one selected from hyaluronic acid and salts thereof in the range of 100 to 100 CS. The viscosity of the aqueous solution of hyaluronic acid (salt) used in the cosmetic of the present invention is determined by a viscosity measuring method of "sodium hyaluronate solution" of the standard of the compounding component for each cosmetic variety. In the present invention, the viscosity is 50 to 100 CS, preferably 60 to 90 CS.

In both the first and second inventions, the amount of hyaluronic acid (salt) added to the cosmetic is 0.0% of the cosmetic.
The proportion is preferably from 5 to 0.20% by weight, more preferably from 0.10 to 0.15% by weight.

The cosmetics referred to in the present invention are rubbing and scattering on the human body in order to clean, beautify, increase attractiveness, change appearance, or keep skin or hair healthy.
Cloth and other materials intended to be used in a similar manner, including both medicinal and non-medical uses. Specifically, foundations, lipsticks, creams and the like can be mentioned.

With the cosmetic of the present invention, it is possible to maintain a sufficient moist feeling and moist feeling without using the above-mentioned auxiliary agents such as polyols, but the effect of the present invention is not impaired. If so, these adjuvants may be added to the cosmetic of the present invention.

Various compounds may be added to the cosmetic of the present invention depending on the purpose of use as long as the effects of the present invention are not impaired. For example, an ascorbic acid derivative may be added for the purpose of whitening effect, and an α-hydroxy acid may be added for the purpose of preventing aging. Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the examples.

[0020]

EXAMPLES Preparation of Cosmetics 100 g of lotion having the composition shown in Table 1 was prepared. Specifically, add the required amount of distilled water to paraben and hyaluronic acid (salt)
(Examples 1 to 6, Examples 11 to 13 and Comparative Examples 1 to 3 are powdered hyaluronic acid (salt) (manufactured by Chisso Corporation,
Product name: Sodium hyaluronate (CHA)
For Examples 7 to 10 and Comparative Examples 4 to 6, a 1% by weight aqueous solution of hyaluronic acid (salt) (manufactured by Chisso Corporation, trade name:
Sodium hyaluronate (CHA) 1% by weight aqueous solution), D
-Examples 1 to 13 and Comparative Examples were prepared by dissolving in the order of sorbitol (manufactured by Wako Pure Chemical Industries, Ltd.) and finally adding a loofah extract (Maruzen Pharmaceutical Co., Ltd., trade name: loofah extract). 1 to 6 lotions were obtained.

[0021]

[Table 1] HA-1: sodium hyaluronate having an average molecular weight of 300,000 ^{* 1} HA-2: sodium hyaluronate having an average molecular weight of 600,000 ^{* 1} HA-3: hyaluronic having an average molecular weight of 700,000 ^{* 1} Sodium hyaluronate HA-4: sodium hyaluronate having an average molecular weight of 800,000 ^{* 1} HA-5: sodium hyaluronate having an average molecular weight of 900,000 ^{* 1} HA-6: sodium hyaluronate having an average molecular weight of 950,000 ^{* 1} HA-7: average molecular weight 1,000,000 ^{* 1} sodium hyaluronate HA-8: Sodium hyaluronate HA-9 with an average molecular weight of 1.2 million ^{* 1} Viscosity of 1% by weight aqueous solution ^{* 2} Sodium hyaluronate HA-10 with 10CS ^* 1: 1% by weight aqueous solution viscosity ^{* 2} : 50 CS sodium hyaluronate HA-11: 1% by weight aqueous solution viscosity ^{* 2} : 60 CS sodium hyaluronate HA-12: 1 weight % The viscosity of the aqueous solution ^{* 2} sodium hyaluronate 90CS HA-13: 1% by weight Viscosity of the aqueous solution ^{* 2} sodium hyaluronate 100 cs HA-14: 1% by weight sodium hyaluronate viscosity ^{* 2} of 130CS aqueous solution 1: Average molecular weight measurement method Pharmacopoeia viscosity measurement method or cosmetic raw material standard viscosity measurement method 1
The limiting viscosity was measured by a method, and the average molecular weight was determined from the following equation using the numerical value. [Η]: Intrinsic viscosity (dl / g) M: Average molecular weight (× 10 ⁴ ) * 2: Method for measuring viscosity of 1% by weight aqueous solution According to the method for measuring the viscosity of “sodium hyaluronate solution” in the standard of the compounding component for each cosmetic type. It was measured. * 3: “Ratio%” means “% by weight”.

2. Evaluation of Cosmetic Sensory evaluation was performed on the lotion obtained by preparing the cosmetic at the time of use and after use by 10 female panelists. Table 2 shows the evaluation results.

[0023]

[Table 2]

[0024]

According to the present invention, if the cosmetic of the present invention contains hyaluronic acid (salt) having an average molecular weight in a specific range or hyaluronic acid (salt) in an aqueous solution having a specific range, an auxiliary agent may be used. A moderate moist feeling and moist feeling can be obtained without addition, and even when using an auxiliary agent such as a polyol, a moderate moist feeling and moist feeling can be obtained with a relatively small amount of addition. Can be.

Claims (6)

[Claims] An average molecular weight of 6 determined by the following formula:
A cosmetic comprising at least one selected from hyaluronic acid and salts thereof in the range of 0 to 1,000,000. [Η]: Intrinsic viscosity (dl / g) M: Average molecular weight (× 10 ⁴ ) 2. The cosmetic according to claim 1, comprising at least one selected from hyaluronic acid having an average molecular weight in the range of 700 to 950,000 and salts thereof. 3. The cosmetic according to claim 1, comprising at least one selected from hyaluronic acid having an average molecular weight in the range of 800,000 to 900,000 and salts thereof. 4. An aqueous solution having a viscosity of 50 to 100 CS.
Selected from hyaluronic acid and salts thereof in the range of
Cosmetics containing more than one species. 5. The content of at least one selected from hyaluronic acid and salts thereof is 0.05 to 0.2 relative to the cosmetic.
The cosmetic according to any one of claims 1 to 4, which is a percentage by weight. 6. The content of at least one selected from hyaluronic acid and salts thereof is 0.1 to 0.15 relative to the cosmetic.
The cosmetic according to any one of claims 1 to 4, which is a percentage by weight.