JP2001139945A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a natural antioxidant usable as a material for medicines, foods, cosmetics, and the like. SOLUTION: This antioxidant is obtained by including a solvent-extract from a plant belonging to Lardizabalaceae. The antioxidant extracted from Akebia quinata Decne is originated from a natural product and has excellent antioxidant property. So it can be applied not only to prevent oxidation of foods, or the like, but also to obtain medicines, foods, cosmetics aiming at the prevention of arteriosclerosis caused by lipid oxidation and the prevention of skin aging, or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a natural product-derived antioxidant which can be used as a pharmaceutical, food or cosmetic material.

[0002]

2. Description of the Related Art Conventionally, free radicals and active oxygen have been considered to have toxic toxicity to living tissues. In particular, recent research has shown that free radicals and active oxygen can damage living bodies, causing various diseases and carcinogenesis. Or aging. At the same time, plants such as vegetables and fruits contain a relatively large amount of "antioxidant components", and antioxidants ingested as food help to eliminate free radicals and active oxygen in the human body It has come to be considered. Therefore, the mechanism of action of a substance having an antioxidant ability extracted from a natural product has been clarified, and development has been pursued for application to medicines, foods, cosmetics, and the like.

[0003] In the Akebiaceae plant to which the present invention is applied, the application of the extract to an antioxidant is not known. The stalk of akebi is called herbal medicine name "Mokutsu (Kidori)", and is said to be effective for inflammation and diuresis, and its water extract has been used. Examples of the use of the organic solvent extract of akebi include those in which a saponin component is used as a drug absorption aid for oral administration for assisting absorption of an orally administered pharmacologically active substance (Japanese Patent Application Laid-Open No. 57-145816). An extract containing hederagenin as an essential component, which provides a cosmetic composition having an excellent whitening effect, an anti-stain effect, and an anti-inflammatory effect (JP-A-64-42411), an extract containing hederagenin as an essential component, There are those which provide a toothpaste composition having an excellent anti-inflammatory effect (JP-A-2-262510) and those which provide an anticancer agent containing a triterpene glycoside as an active ingredient (JP-A-60-8224). Thus, the extract of akebi is only used for anti-inflammatory action, diuretic action, whitening action and the like.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to provide an antioxidant derived from a natural product which can be used as a material for pharmaceuticals, foods, cosmetics and the like.

[0005]

The present inventors have conducted intensive studies on the antioxidant activity of extracts of vegetables and herbal medicines which have been used for the human body for a long time and are considered to have few problems in safety. Surprisingly, the present inventors have found that the organic solvent extract of akebi has a high antioxidant activity, and completed the present invention. That is, the present invention is an antioxidant characterized by containing an extract of a plant of the family Akebiaceae. At this time, the Akebiaceae plant is Akebia (Akebia quinata D
ecne) or a congener thereof (Akebiaceae: Akebiaceae) is preferable, and the site of the Akebiaceae plant is preferably a fruit, and the solvent used for extraction is particularly preferably dimethyl ether.

[0006] Examples of the Akebiaceae plants used as the extraction raw material of the present invention include Akebia and Stauntonia. Species belonging to the genus Akebia include Akebia (Akebia quina
ta Decne), Akebia trifoliata Koizdu
mi), Akebia pentaphylla Makino, and other species belonging to the genus Mbe.
hylla Decne). The site to be used may be any site such as fruit (peel, pulp, seed, etc.), stem, root, leaf, etc. Preferably, the akebi (Akebi quinataDecne)
) Or its congener plants, such as honeysuckle and sycamore, or the fruit containing seeds.

[0007] The type of the extraction solvent is not particularly limited, but alcoholic solvents such as methanol, ethanol, propanol, isopropanol, butanol and isobutanol; ether solvents such as dimethyl ether, diethyl ether and methyl ethyl ether; Ester solvents such as butyl acetate and ethyl propionate, ketone solvents such as acetone, chloroform,
Examples thereof include polar solvents such as a chlorine-based solvent such as dichloromethane, and these can be used alone or as a mixture of two or more kinds, or mixed with water. Preferably, ethanol which is highly safe for the human body and is generally used for food production,
Alternatively, dimethyl ether which does not cause the solvent to remain is selected. Particularly, dimethyl ether is preferred because an extract having high antioxidant activity can be obtained.

[0008] The extraction operation is performed by a conventional method. That is, 5 parts to 20 parts of the extraction solvent is used for 1 part by weight (dry weight) of the pulverized raw material.
It is performed by adding a double volume. Raw materials may be raw or dried. The extraction is generally performed at around room temperature of 10 to 40.
C. for 30 minutes to 2 hours. After extraction, the extraction residue is separated by a separation operation such as filtration or centrifugation to obtain an extraction filtrate. Also, a Soxhlet extractor can be used. The extract filtrate is concentrated to obtain an extract. In order to increase the antioxidant activity, purification can be performed by a general method such as solvent fractionation or column fractionation. The target antioxidant can be obtained by evaporating the solvent from the obtained purified product to dryness (freeze drying, spray drying, etc.). Freeze-drying, which can be dried preferably at low temperature and under conditions of low contact with oxygen, is desirable. In the process of evaporative drying,
Excipients such as crystalline cellulose, starch, and lactose can also be mixed.

The thus-obtained akebi extract has excellent antioxidant activity derived from natural products, and thus prevents diseases considered to be caused by free radicals or active oxygen, such as colon cancer. Can be expected to prevent carcinogenesis, arteriosclerotic diseases such as myocardial infarction and ischemic heart disease, neurological diseases such as Parkinson's disease and Alzheimer's disease, as well as eye diseases and kidney diseases. In addition, effects such as prevention of skin aging caused by lipid oxidation can be expected. That is, the antioxidant of the present invention can be used in medicines, foods, cosmetics, and the like for the purpose of preventing not only oxidation of foods and the like but also arteriosclerosis and skin aging caused by lipid oxidation. The antioxidant of the present invention can be used in combination with other plant extracts such as vitamin E, vitamin C, licorice, and other antioxidants such as BHT.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to experimental examples, examples, and application examples, but these examples do not limit the present invention in any way.

[Experimental example 1] Akebia berries were mixed with water, hexane, ethanol,
Extraction was performed with dimethyl ether, and the antioxidant activity of each extract was compared. The raw material for the extraction is dried akebi (9% moisture)
Obtained by pulverizing to about 5 mm or less. For dimethyl ether extraction, 200 g of the raw material was placed in a 5 L pressure vessel equipped with a stirrer, and after 3,000 ml of dimethyl ether was injected, 30 g of dimethyl ether was added.
After performing stirring extraction at 30 ° C. for 30 minutes, the mixture was filtered with a sintered metal and received in another pressure vessel. Dimethyl ether was evaporated from the filtered extract at 30 ° C. This concentrated liquid was freeze-dried at room temperature for 24 hours to obtain 10.3 g of an extract. For water extraction, hexane extraction and ethanol extraction, 200 g of each raw material was placed in a 5 L pressure vessel equipped with a stirrer, 3,000 mL of a solvent was added, and the mixture was stirred and extracted at 30 ° C. for 30 minutes, and then filtered through a membrane filter (pore size 0.2 μm). Then, the extraction filtrate was recovered. The extract filtrate was concentrated in vacuo using an evaporator, and then freeze-dried at room temperature for 24 hours to obtain 59.9 g, 1.4 g and 15.0 g of the extract, respectively.

The antioxidant activity is measured by the following method according to "Reference 2.5-1996 Stability test for autoxidation, oven test (gravimetric method)" of the standard fat and oil analysis test method (edited by the Japan Oil Chemists' Society). went. 50 mg of the extract was added to 5 g of the linoleic acid reagent, and the mixture was stirred and mixed at 60 ° C. for 30 minutes under vacuum to make it uniform. 1 g of this liquid is placed in a Petri dish (3.5 c
m) was accurately measured and the weight increase in an oven at 55 ° C. was measured over time. The weight was measured once a day, and the time when the rate of weight increase reached 0.5% was defined as the oxidation time of the sample. Separately, a linoleic acid solution to which no composition was added was treated in the same manner, and the blank oxidation time was set. The antioxidant activity of the sample
The time was obtained by subtracting the oxidation time of the blank from the oxidation time of the sample. Table 1 shows the measurement results of the antioxidant activity. Extraction with water and hexane showed almost no antioxidant activity, and an extract having high antioxidant activity was obtained with polar solvents of ethanol and dimethyl ether. In particular, an extract with dimethyl ether showed high antioxidant activity.

[0012]

[Table 1]

In the following examples, a dried product of Akebi (9% moisture) was ground to about 5 mm or less.

Example 1 200 g of raw materials and 150 ml of water were placed in a 5 L pressure vessel equipped with a stirrer, and dimethyl ether 3000 m
After pressurized injection, the mixture was stirred and extracted at 30 ° C. for 30 minutes, filtered off with a membrane filter (pore size 0.2 μm), and received in another pressure vessel. The extract separated by filtration is 30 ° C.
Under, the dimethyl ether was evaporated. This concentrated liquid was freeze-dried at room temperature for 24 hours to obtain 15.0 g of a clay-like extract. The antioxidant activity of this extract shown in Experimental Example 1 was 180 hours.

[0014]

Example 2 200 g of raw materials and 150 ml of water were put into a 5 L pressure vessel equipped with a stirrer, and dimethyl ether 3000 m
After pressurized injection, the mixture was stirred and extracted at 30 ° C. for 30 minutes, filtered off with a membrane filter (pore size 0.2 μm), and received in another pressure vessel. The extract separated by filtration is 30 ° C.
Under, the dimethyl ether was evaporated and concentrated to 500 ml. After leaving this concentrated liquid to separate into two phases, the aqueous layer was removed. The obtained dimethyl ether phase was concentrated under reduced pressure, and freeze-dried at room temperature for 24 hours to obtain 6.3 g of a powdery extract. The antioxidant activity of this extract shown in Experimental Example 1 was 315 h.

[0015]

Example 3 200 g of raw materials and 150 ml of water were placed in a 5 L pressure vessel equipped with a stirrer, and dimethyl ether 3000 m
After pressurized injection, the mixture was stirred and extracted at 30 ° C. for 30 minutes, filtered off with a membrane filter (pore size 0.2 μm), and received in another pressure vessel. The extract separated by filtration is 30 ° C.
Under, the dimethyl ether was evaporated and concentrated to 500 ml. After leaving this concentrated liquid to separate into two phases, the aqueous layer was removed. 100 m of water in the remaining dimethyl ether layer
After press-fitting and mixing, the mixture was allowed to stand and separated into two phases.
The aqueous layer was removed again, and the obtained dimethyl ether phase was concentrated under reduced pressure and freeze-dried at room temperature for 24 hours to obtain 5.4 g of a powdery extract. The antioxidant activity of this extract shown in Experimental Example 1 was 497 h.

[0016]

[Application Example 1] Health food An extract obtained in the same manner as in Example 3 was dissolved in ethanol so that the solid content concentration was 10% by weight. Next, 30 parts by weight of this solution was added to crystalline cellulose (Avicel <
(Registered trademark> FD-101, manufactured by Asahi Kasei Kogyo Co., Ltd.) and mixed well, followed by drying. This powder 33
Parts by weight (containing 3 parts by weight of extract and 30 parts by weight of crystalline cellulose), 35 parts by weight of erythritol (manufactured by Niken Kagaku Co., Ltd.), 30 parts by weight of maltitol (manufactured by Towa Chemical Industry Co., Ltd.), sucrose fatty acid Esters (DK ester F-20W,
After 2 parts by weight of Daiichi Kogyo Seiyaku Co., Ltd.) were mixed well, a rotary tableting machine (Kikusui Seisakusho, CLEANPR)
8mmφ in ESS CORRECT 12HUK), 1
Using a 2R punch, turntable rotation speed 12 rpm
To give a tablet weighing 200 mg.

[0017]

[Application Example 2] Cosmetic (oil phase) stearic acid 16.0% Extract obtained in the same manner as in Example 3 0.2% Powdered egg yolk oil (phospholipid 30%) 4.0% POE (20 ) Sorbitan stearate 1.0% (aqueous phase) Propylene glycol 10.0% Water 68.0% Methyl paraben 0.1% Oil phase components were kept at 70 ° C, well dissolved and mixed, and kept at 70 ° C therein. The aqueous phase was dropped and mixed, and simultaneously emulsified uniformly with a homomixer. After emulsification, stirring was continued while cooling, and when the temperature reached 30 ° C, a colloid mill treatment was performed to obtain a cream.

[0018]

The antioxidant extracted from the akebi of the present invention has excellent antioxidant activity derived from natural products.
It can be used for medicines, foods, cosmetics, etc. for the purpose of preventing oxidation of foods and the like, as well as for preventing arteriosclerosis and skin aging caused by lipid oxidation.

Claims (4)

[Claims] 1. An antioxidant comprising an extract of a plant of the family Akebiaceae. 2. The plant of the family Akebia is Akebia quina.
The antioxidant according to claim 1, which is a plant of the same genus (Acebiaceae). 3. The antioxidant according to claim 1, wherein the site of the Akebiaceae plant is a fruit. 4. The antioxidant according to claim 1, wherein the solvent used for the extraction is dimethyl ether.