JPH053453B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a composition for inhibiting the production of lipid peroxide in vivo, and more specifically, the present invention relates to a composition for inhibiting the production of lipid peroxide in vivo, and more specifically, it contains an extract of herbal medicine having antioxidant ability, and is used to prevent various diseases caused by lipid peroxide in vivo. The present invention relates to an in-vivo lipid peroxide production inhibitor composition that is aimed at: In recent years, there has been remarkable progress in research on lipid peroxides in vivo, and the physiological tendencies and pathological effects of lipid peroxides have been studied, such as in organs such as the brain, heart, blood vessels, lungs, liver, skin, eyes, and blood cells including platelets. It has been revealed that lipid peroxides are involved in some of the functions and lesions of the human body. Lipid peroxides can cause cerebrovascular disorders such as cerebral infarction, retinopathy of prematurity, iron disease of the eyeball or cornea, ischemic heart disease such as myocardial infarction or angina pectoris, radiation pneumonitis, emphysema, and the liver. Carbon tetrachloride liver damage, post-halothane anesthesia liver damage,
Severe hepatitis, decompensated liver cirrhosis, fatty liver, alcoholic hepatitis, poorly controlled diabetes, diabetic angiopathy, type A familial hypercholesterolemia, gout, female melasma, melasma, armpit odor, skin cancer. It is known to be a cause of various diseases such as preeclampsia, preeclampsia, and terminal cancer. Several drugs are known to improve these in vivo lipid peroxidation abnormalities, such as tocopherols, ascorbic acid, glutathione, carotenoids, superoxide desmutase, catalase, and glutathione peroxidase, but these drugs do not necessarily It wasn't satisfying. As a result of various studies on in-vivo lipid peroxide production inhibitors under such circumstances, the present inventors have discovered that when a specific herbal medicine extract is administered to the in-vivo, the in-vivo production of lipid peroxide can be extremely suppressed. We have completed an inhibitor of lipid peroxide production in vivo. That is, the present invention provides an in vivo lipid peroxide production inhibitor composition containing one or more selected from rosemary extract, salvia extract, and allspice extract. Rosemary extract, salvia extract, and allspice extract (hereinafter simply referred to as crude drug extracts), which have antioxidant ability, are used as food additives and have extremely low toxicity. The crude drug extract is prepared by a conventional method using water, a hydrophilic organic solvent, a water-containing hydrophilic organic solvent, other organic solvents, liquid edible oil, etc. as an extraction solvent. Solvents used for extracting crude drug extracts include ethanol, acetone, methanol,
Examples include ethylene chloride, ethyl ether, propylene glycol, glycerin, n-hexane, petroleum ether, petroleum benzene, water, liquid edible fats and oils, etc. From the standpoint of safety and operability, ethanol, acetone, n-hexane, water , liquid edible fats and oils are particularly preferred. These solvents can be used alone or in combination. For example, when a mixed solution of a polar solvent and water is used, the concentration of the polar solvent is preferably 40% by weight (hereinafter expressed as %) or more, particularly 60% or more. Further, when a mixture of a polar solvent and a non-polar solvent is mixed with water and used, the concentration of the mixture of a polar solvent and a non-polar solvent may be 5% or more. This extraction step can be carried out at room temperature for 1 hour or more, or with heating if necessary, using a solvent in an amount equal to or more than the same weight as the dried crude drug as the raw material. Generally, the extract is obtained by distilling off the solvent from this extract, but if the solvent used for the extraction process is ethanol, glycerin, propylene glycol, or a mixture of these and water,
The extract can also be used as is. The in vivo lipid peroxide production inhibitor of the present invention includes crude drug extracts alone or drugs that are already known to improve in vivo lipid peroxide abnormalities.
For example, tocopherols, ascorbic acid, glutathione, carotenoids, superoxide desmutase, catalase, glutathione peroxidase, etc. can be mixed with excipients, additives, bases, etc. necessary for formulation, and formulated using conventional methods. can. In order to enhance the results, the in vivo lipid peroxide production inhibitor of the present invention is preferably administered as an oral preparation such as a tablet, pill, granule, drink, or capsule. If desired, it can also be administered as parenteral preparations such as injections, suppositories, and ointments. That is, the dosage form of the inhibitor of the present invention is:
It may be selected as appropriate depending on the type of disease. The dosage of the in vivo lipid peroxide production inhibitor of the present invention varies depending on the type of disease and administration method, but 1
1 mg/Kg to 100 mg/Kg per day is appropriate. Next, the present invention will be explained with reference to Examples. Example 1 Acetone extracts of rosemary, salvia, and allspice were produced, and their ability to inhibit lipid peroxide production was tested by the following method. [Manufacture of rosemary acetone extract] Add 3 kg of acetone to 1 kg of rosemary powder,
After extraction for 3 hours at room temperature, the extract is filtered. The filtrate was concentrated using a rotary evaporator to completely remove the solvent, yielding 170 g of a rosemary extract with antioxidant ability. [Manufacture of salvia acetone extract] Add 3 kg of acetone to 1 kg of salvia powder and perform the same operation as in the case of rosemary above to obtain 145 g of a salvia extract having antioxidant ability. [Manufacture of allspice acetone extract] Adding 3 kg of acetone to 1 kg of allspice powder and performing the same procedure as for rosemary above produces an allspice extract with antioxidant properties.
Get 120g. [Test method] SD male rats (11 weeks old, average weight approximately 300g)
The rats were fed a diet containing 0.1% of the rosemary, salvia, and allspice extracts for one week (Table 1), and then fed with a mixture of carbon tetrachloride (a substance derived from lipid peroxide) and liquid paraffin (1%). :
1, V/V) was orally administered at 2.5 ml/Kg. Note that the same amount of liquid paraffin was used as a control. Three hours after administration, the animals were sacrificed, blood was collected, and the liver was perfused with ice-cold physiological saline through the portal vein. Lipid peroxide, GOT, and GPT in the obtained serum were determined by Wako Pure Chemical Industries.
Co., Ltd.'s measurement kit, and the Yagi method (Ohokawa, H., et al., Anal.
Biochem., 95 , 351-358 (1979)). The results are shown in Table 2.

【table】

[Table] Isu extract.

[Table] As shown in Table 2, an increase in lipid peroxide was observed in the liver tissue due to carbon tetrachloride administration, but this increase was suppressed by preadministration of rosemary, salvia, or allspice extract. Also GOT,
The increase in GPT was also significantly suppressed, indicating that rosemary extract is effective in preventing liver damage thought to be caused by an increase in lipid peroxide. Example 2 The acetone extract of rosemary used in Example 1 was thoroughly dried, mixed with 5 times the amount of purified medium-chain fatty acid triglyceride (Example 3) and an equal amount of natural vitamin E, and prepared using a conventional method. Fill 100mg into soft gelatin capsules. Example 3 Add 2.5 kg of ethanol and 2.5 kg of water to 1 kg of Saalvia, extract at 45°C for 5 hours, filter the extract,
Obtain 4.5 kg of Salvia ethanol-water extract.
This extract is used to produce a beverage that suppresses lipid peroxide production in vivo. Composition: Ring vinegar 10.0 parts by weight Citric acid 5.0 Ascorbic acid 0.5 Caffeine 0.5 Granulated sugar 130.0 Honey 20.0 Salvia extract 100.0 Mineral water balance 1000 After thoroughly stirring and dissolving ~, filter, pack in a bolt, and use as a sterilized product. Example 4 Add 3 kg of n-hexane to 1 kg of allspice, extract at room temperature for 12 hours, and then filter the extract. The solvent is completely removed from this liquid using a rotary evaporator to obtain 190 g of an allspice extract having antioxidant ability. Using this extracted powder,
Manufacture tablets that inhibit in vivo lipid peroxide production. Allspice extract powder 84.2%, corn tarch 4.0, crystalline cellulose 8.3, carboxymethyl cellulose calcium 3.5 were mixed well and processed by dry granule compression method.
Compress into 400mg tablets and take 3 tablets a day. Comparative Example A feed was prepared in the same manner as in Example 1 except that ascorbic acid and tocopherol were added in place of the rosemary acetone extract in Example 1. Next, an experiment was conducted according to the method of Example 1, and serum lipid peroxide and liver peroxide lipid were measured. As shown in Table 3, no significant difference was found between them and the control.

【table】

Claims (1)

[Scope of Claims] 1. An in vivo lipid peroxide production inhibitor composition containing one or more selected from rosemary extract, salvia extract, and allspice extract. 2. The in vivo lipid peroxide production inhibitor composition according to claim 1, wherein the dosage form is an oral preparation.