JP2001046051A - Method and apparatus for producing d-cysteinolic acid - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for efficiently supplying a large amount of D-cysteinolic acid, and further to provide an apparatus therefor. SOLUTION: This method for producing D-cysteinolic acid comprises (1) a step for culturing sea lettuce containing the D-cysteinolic acid in the first culturing vessel opened at the upper part while exchanging a culture solution in the culturing vessel with seawater in the exterior of the culturing vessel, and bubbling the solution in the culturing vessel with carbon dioxide gas, (2) a step for further culturing the cultured and collected sea lettuce in the second culturing vessel while adding an sulfur compound and/or a peroxide to increase the content of the D-cysteinolic acid in the sea lettuce, (3) a step for drying the cultured, collected and washed sea lettuce under a shaded condition, and (4) a step for extracting the objective D-cysteinolic acid from the dried sea lettuce with an organic solvent or hot water, optionally purifying the extracted solution by an ion-exchange resin, and crystallizing the D-cysteinolic acid in the coexistence of ethanol or methanol. The apparatus therefor is also provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a technique for producing D-cysteinolic acid used as an additive for feeds of medicines, pharmaceuticals, health foods, livestock and fish.

[0002]

2. Description of the Related Art D-cysteinolic acid (C ₃ H ₉ NO ₄ S: molecular weight 155) has been known to be contained in trace amounts in sardines.

[0003]

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D-cysteinolic acid is expected to be used as a raw material for pharmaceuticals because it is a sulfur-containing amino acid having an antithrombotic effect. As a therapeutic and prophylactic agent for thrombosis, a patent for a therapeutic and prophylactic agent for thrombosis of D-cysteinolic acid extracted from sardine has been filed (Japanese Patent Application No. 61-47880). Further, a patent has been filed for a cholesterol-lowering agent containing cysteinolic acid or a bile acid conjugate thereof for the purpose of lowering blood cholesterol (Japanese Patent Application No. 3-49).
113 etc.).

[0005] D-cysteinolic acid was obtained from BW in 1957.
Since Ickberg isolated and identified red seaweed, Ito of Hiroshima University in 1963 revealed that it was present in green algae and brown algae in seaweed. After that, 19
In 1965, D-cysteinolic acid derived from the starfish Starfish was isolated. In 1979, Yoneda of Hokkaido University derivatized D-cysteinolic acid to separate it from taurine.

The above-mentioned conventional method has the following problems.

(1) D-cysteinolic acid has been purified from sardines as a raw material. However, since its content is very small, the maximum level of recovery is from 100 kg to 12 g of sardines. Dozens of grams were recovered from ton-order sardines. The yield is up to about 0.01%, the recovery is low, and large amounts of fresh sardines are required for extraction, making it very difficult to produce D-cysteinolic acid from natural materials on an industrial scale. Had become.

(2) Also, as described above, D-
Although it was clear that cysteinolic acid was present, culturing seaweed, which is a raw material for D-cysteinolic acid, would mean that production throughout the year would mean that the seaweed would mature during the culturing process and the shape of the seaweed would collapse. It was difficult to do. In addition, since little is known about the abundance, the current situation is that active production of D-cysteinolic acid from seaweed has not been achieved.

[0009]

SUMMARY OF THE INVENTION In view of the above problems, an object of the present invention is to provide a method for efficiently supplying D-cysteinolic acid in a large amount, and an apparatus therefor.

[0010]

In order to achieve the above object, the following means are employed.

(1) In a first culture tank having an open upper part,
While supplying seawater containing nitrogen and phosphorus into the culture tank and bubbling carbon dioxide gas into the culture tank, D-
A method for producing D-cysteinolic acid, comprising a step of culturing Aosa containing cysteinolic acid and a step of extracting D-cysteinolic acid from the Aosa with an organic solvent or hot water.

(2) After culturing in the culturing step described in (1), recovering the Ulva; and adding a sulfur compound and / or a peroxide to the collected Ulva, in the second culture tank. A step of culturing so as to increase the content of D-cysteinolic acid in the Ulva, and a step of extracting D-cysteinolic acid from the Ulva with an organic solvent or hot water. A method for producing cysteinolic acid.

(3) After collecting the Ulva produced by the culture method according to (1) or (2), drying the Ulva under a light-shielded environment; Extracting with a solvent or hot water.

(4) a step of further purifying D-cysteinolic acid obtained by the production method according to any one of (1) to (3) with an ion exchange resin and crystallizing the same in the presence of ethanol or methanol; A method for producing D-cysteinolic acid, comprising:

(5) The method according to any one of (1) to (4), wherein the Aosa is a sterile Aosa.

(6) A first culturing apparatus for cultivating Aosa containing D-cysteinolic acid, comprising a first culturing tank having an open upper part, and nitrogen and phosphorus contained in the culturing tank. Supply means for supplying seawater to be supplied and a stirring means, and a first culturing apparatus comprising an aeration means for bubbling carbon dioxide gas in the culturing tank; and after culturing in the first culturing apparatus, A second culturing apparatus for culturing the collected Ulva, comprising: a second culturing tank having no perforated wall; and an adding means for adding a sulfur compound and / or a peroxide. A culturing device; a drying device for drying the blue seaweed after collecting the blue seaweed produced in the first culturing device or the second culturing device, wherein the drying device has a structure for preventing direct sunlight, Drying room with an opening and closing part for A drying device comprising a heat and dehumidifying means; and extracting D-cysteinolic acid from the blue seaweed treated by the device according to any one of the first culture device, the second culture device, and the drying device. An extractor having a means for stirring with an organic solvent or hot water.

(7) The production apparatus according to (6), further comprising: a purification means using an ion exchange resin for purifying D-cysteinolic acid from the extract obtained by the extraction apparatus; An apparatus for producing D-cysteinolic acid, comprising: means for crystallization in the coexistence.

[0018]

BEST MODE FOR CARRYING OUT THE INVENTION The present inventors have conducted intensive analysis and evaluation of a material containing a large amount of D-cysteinolic acid mainly on seaweed, and have found the following.

(1) It was confirmed that D-cysteinolic acid was present in seaweed green algae Aosa (genus Ulva), and in particular, sterile Aosa (Ulva lactuc) which can be cultured and produced all year round.
It was found that the limited individuals in a) contained D-cysteinolic acid in a large amount of 0.1 to 0.6% by dry weight.

(2) In addition, a peroxide such as hydrogen peroxide is added together with a sulfur compound, which is a component of D-cysteinolic acid, in the process of culturing the selected sterile blue seaweed. It has been found that the content of D-cysteinolic acid is improved.

(3) In the step of extracting D-cysteinolic acid from sterile blue seaweed, a condition for drying blue seaweed under light-shielding without decomposing and losing D-cysteinolic acid was found.

The present invention has been made by finding these (1), (2) and (3). That is, an object of the present invention is to provide a method for simply and efficiently producing D-cysteinolic acid. In addition, the mutant strain called sterile Aosa can be cultured for the entire year without maturation and generational change in a specific season as compared with the conventional one, so that the D-cis Mass production of tenolic acid can be expected.

The characteristics of the sterile Aosa that can be used for the production of D-cysteinolic acid of the present invention are shown below.

This sterile Aosa was collected from the coast of Yokohama City. The present inventor has designated this strain as sterile Aosa MHI-AT.
The strain was named RC-1 and submitted for deposit at the National Institute of Bioscience and Human-Technology, National Institute of Advanced Industrial Science and Technology.

Shape: Cloth-like and floating. Size: 1 cm large to 100 cm square. Mobility: None. Nitrogen source: Able to grow with various types of ammonia nitrogen, urea nitrogen, nitrate nitrogen. Nitrate nitrogen is preferred for increasing the D-cysteinolic acid content. Photosynthetic pigments: chlorophyll a, chlorophyll b, carotenoid (detected by thin layer chromatography) Proliferation: culture using a solar simulator with aeration containing 0.1% CO ₂ at 25 ° C in the presence of 1.5 ppm nitrogen (12 h light) / 12h dark), about 0.3g (dry weight /
Liters / day).

Hereinafter, a method for producing D-cysteinolic acid from Aosa containing D-cysteinolic acid will be described together with the apparatus used. The method and apparatus for producing D-cysteinolic acid of the present invention can be used for all seaweeds containing D-cysteinolic acid. However, in terms of production efficiency, it is preferable to use sterile Aosa. Fertility Aosa MHI-
More preferably, the ATRC-1 strain is used.

First, Aosa containing D-cysteinolic acid is first cultured in a culture tank having an open top for the purpose of mass production of D-cysteinolic acid. The first culture is preferably performed for 7 to 14 days.

The culture tank used in the first culture may itself have a structure capable of supplying seawater containing nitrogen and phosphorus into the culture tank, or may further include a means for supplying the seawater. You may.

The structure in which the culture tank can supply the seawater by itself is, for example, a structure like a living cage in which the culture tank is suspended on the seawater, and the culture tank is capable of supplying the seawater. Means include those having perforated walls.
The perforated wall allows exchange of the seawater outside the culture tank with the culture solution in the culture tank.

Further, the structure further provided with the seawater supply means means that the culture tank has no perforated wall,
Separately, a means for supplying the seawater is provided. In this case, the nutrient source of Aosa is ensured by replenishing and exchanging the seawater containing nitrogen and phosphorus into the culture tank using a supply means. The exchange of seawater can be performed at a flow rate of about 2 to 5 L / seawater volume L · hr when the dissolved nitrogen in the seawater is about 1 ppm.

In the present invention, as the seawater containing nitrogen and phosphorus, either eutrophicized seawater or seawater to which nitrogen and phosphorus are added can be used.

The culture tank further comprises means for stirring the culture solution in the culture tank, and ventilation means for bubbling carbon dioxide gas into the culture tank. The stirring means is not particularly limited as long as it can stir the seawater outside the culture tank and the culture solution in the culture tank into a uniform state. As an example, a jet streamer can be used, for example. Bubbling of carbon dioxide gas can be performed by supplying a gas obtained by adding 0.1 (v / v)% CO ₂ to air at a rate of about 3 to 8 L / seawater capacity L · hr. By the stirring means and the aeration means, nitrogen, phosphorus, and carbon dioxide, which are nutrient sources of Aosa, are efficiently supplemented from seawater outside the culture tank.

As described above, Aosa cultivated in large quantities using components in seawater as a nutrient source is preferably recovered from seawater using, for example, a net-like or monkey-like basket, and then D-
It is transferred to a second culture step to improve the cysteinolic acid content. The recovery step is not particularly limited, as long as the Ulva individual can be isolated from seawater.

In the second culturing step, D
-In order to increase cysteinolic acid content,
Aosa is cultured with sulfur compounds and / or peroxides that are the source of D-cysteinolic acid. The second culture is preferably performed for 1 to 3 days.

The culture vessel used in the second culture can be any one as long as it does not have a perforated wall, that is, has a complete partition from the outside of the culture vessel. This can prevent the added sulfur compound and / or peroxide from diffusing out of the culture tank, and maintain the substance at a high concentration in the culture tank.

The culturing tank is further provided with a means for adding a sulfur compound and / or a peroxide. The addition means is not particularly limited as long as a sulfur compound and / or a peroxide can be added to the culture tank at a desired concentration. For example, most substances to be added can be introduced and dissolved as an aqueous solution or as a granular solid. In addition, some peroxides can be dissolved and introduced as a gaseous substance by bubbling.

The sulfur compound is preferably a sulfate,
For example, magnesium sulfate, potassium sulfate, ammonium sulfate and the like can be mentioned, and the added sulfate ion concentration is preferably 1.1 to 1.5 times that of seawater. Examples of the peroxide include hydrogen peroxide and ozone. The concentration at the time of injection is preferably 0.2 to 10 ppm for hydrogen peroxide and 0.01 to 0.1 ppm for ozone.

After the first or second cultivation step, Aosa is washed before the next drying step.

After washing, Aosa is preferably dried in a light-shielded environment in order to prevent decomposition of D-cysteinolic acid produced by Aosa. The light-shielded environment is not particularly limited as long as it is an environment that can block direct sunlight, and may be an indoor space or an open space that blocks direct sunlight. The drying process is completed in approximately 24-72 hours.

The drying apparatus used has a drying chamber having a structure for protecting direct sunlight and having an opening / closing section for ventilation, and a heating / dehumidifying means. The opening / closing unit for ventilation is not particularly limited as long as the opening / closing unit can be opened to promote ventilation. For example, the opening / closing unit can be located on the side surface of the drying device. Drying can also be promoted using a fan. The heating temperature is preferably set to 70 ° C. or less and controlled to 40 to 60 ° C. in order to prevent the decomposition of D-cysteinolic acid. Simultaneously with the heating, dehumidification may be performed to increase the drying efficiency.

With the structure of the drying device that blocks direct sunlight, Aosa can be efficiently dried by a structure that promotes ventilation and a heating / dehumidifying means while suppressing the decomposition of D-cysteinolic acid.

Next, the step of extracting D-cysteinolic acid from Aosa after the first or second culture step or dried Aosa is performed by stirring Aosa in an organic solvent or hot water. The extraction is completed in about 1-2 hours. At this time, in order to increase the extraction efficiency, it is preferable to grind Aosa to several millimeters. The hot water is used at a temperature of 50 to 70 ° C. in order to prevent the decomposition of D-cysteinolic acid.
% Ethanol or methanol solutions can be used.

After the above extraction, after decolorization with diethyl ether,
Activated carbon is added to adsorb the dye, and then a clear solution can be obtained by filtration. By concentrating this solution, D-cysteinolic acid is partially purified.

In order to obtain D-cysteinolic acid having a higher purity, in addition to the above-mentioned procedure, purification by an ion exchange resin is performed, and then crystallization is performed in the presence of 70 to 80 (v / v)% ethanol or methanol. It is preferred to perform the operation. In the purification using an ion exchange resin, a strongly acidic cation exchanger and a weakly basic anion exchanger can be used. In the crystallization step, crystals of D-cysteinolic acid can be obtained by storing at 75 ° C (v / v)% ethanol or methanol at ice temperature.

By the above steps, the content of D-cysteinolic acid in blue seaweed is increased, and then the extraction efficiency is increased, so that the yield of D-cysteinolic acid can be finally improved.

FIG. 1 shows an example of the process for producing D-cysteinolic acid according to the present invention using the above sterile Aosa MHI-ATRC-1 strain.

The Aosa culture production means 1 has a structure like a fish cage in which a culture part having an open top is floated on the sea, and the bottom of the culture part is a perforated wall. The culturing unit is provided with a means for stirring the seawater in the culturing unit and an aeration unit in the vicinity of the bottom of the culturing unit immediately below the perforated wall. The agitation means and the aeration means promote the exchange of seawater inside and outside the culturing section, thereby enabling nitrogen and phosphorus in the seawater to be effectively used as nutrient sources for Aosa. The ventilation line, which is installed at almost the same water level as or below the perforated partition wall that forms the bottom of the culture section, has a number of discharge holes, gently agitates the seawater in the culture tank, and produces carbonic acid, a nutrient source of Aosa. It has the effect of supplementing the gas and promoting the exchange of seawater in the culture section with seawater rich in external nutrients. An electric or internal combustion engine type compressor or the like can be used as the ventilation means. In an internal combustion engine, part or all of the carbon dioxide in the exhaust gas is supplied to the ventilation line to increase the concentration of carbon dioxide in the ventilation line, thereby having the effect of promoting the growth of Ulva. Further, carbon dioxide gas can be drawn in from another carbon dioxide gas generation source and used.

The Ulva collecting means 2 is for collecting the grown Ulva. As a method of recovery, the seawater containing Aosa is pumped up, and the seawater and Aosa are separated by a net-shaped basket having an appropriate pore size so that Aosa does not leak, or the Aosa is picked up in a colander-shaped basket and harvested. , The collection method can be selected according to the size of Aosa.

The D-cysteinolic acid content improving means 3 is composed of an independent Aosa culture tank having a partition completely from seawater at sea, and the Aosa produced by the Aosa culture production means 1 is collected by the recovery means 2. The mixture is transferred to the means 3 and cultured in high density of Aosa in the presence of the following additives. Examples of the compound to be added to improve the content of D-cysteinolic acid include, for example, peroxides such as hydrogen peroxide and ozone, and the concentration at the time of injection is 0.2 to 10 ppm of hydrogen peroxide, Is preferably 0.01 to 0.1 ppm.
As the sulfur compound to be added, a sulfate is desirable,
For example, magnesium sulfate, potassium sulfate, ammonium sulfate and the like can be mentioned, and the added sulfate ion concentration is preferably 1.1 to 1.5 times that of seawater. By adding these peroxides and sulfur compounds, the content of D-cysteinolic acid can be increased, and the amount used can be reduced because of the independent structure that separates from other culture tanks.

The sea urchin washing means 4 is installed on land and removes salt of sea urchin collected from seawater by washing with fresh water. It has the function of increasing.

Aosa drying means 5 is provided for the purpose of preserving Aosa and preventing the decomposition of D-cysteinolic acid contained in Aosa, and is provided with a heating / dehumidifying device.
Aosa can promote drying. Heating temperature is 40 ~
It is desirable to control the temperature to 70 ° C. or less, since D-cysteinolic acid is decomposed at a higher temperature. In addition, an opening / closing unit is provided at a position on the side surface of the structural unit. When the weather is fine, the opening / closing unit is opened to promote ventilation and promote drying of Aosa. However, it has a structure in which direct sunlight does not enter, and suppresses decomposition of D-cysteinolic acid due to direct sunlight. In addition, the sample after dried Aosa can be cut and formed and used as feed for livestock and health food.

The D-cystenoic acid extraction means 6 is used to convert the blue-grained Aosa to hot water of 50 to 70 ° C. or 50 to 70 ° C.
Is stirred for 1 hour with a 60-75 (v / v)% ethanol solution or the same methanol solution, and D-cysteinolic acid is extracted from Aosa into the solution by this operation.
Next, after decoloring with diethyl ether, the aqueous layer is charged with activated carbon and filtered to obtain a transparent liquid. D-cysteinolic acid is contained in the supernatant thus obtained, and partially purified D-cysteinolic acid can be recovered only by drying under reduced pressure. In this state, most of the components other than the mixed peptides and some dyes can be separated. If higher purity D-cysteinolic acid is required, it is purified using a strongly acidic cation exchanger and a weakly basic anion exchanger, and then 75 (v / v)
Crystals of D-cystenoic acid can be obtained by storage at% ice or methanol at ice temperature. As described above, D-cysteinolic acid can be purified and isolated from sterile blue seaweed produced by the above-described means 1 to 6.

[0053]

The method of the present invention will be described more specifically with reference to the following examples.

(Example 1) In order to reproduce Aosa production in the ocean on land, Aosa MHI-ATRC was used using seawater with a dissolved nitrogen concentration of about 1 ppm obtained from the vicinity of Hakkeijima, Kanazawa-ku, Yokohama-shi.
The strain was cultured in a solar simulator (manufactured by Wacom Inc. using a xenon lamp as a light source). The culture was performed by the following operation.

A gas obtained by adding 0.1 (v / v)% CO ₂ to air is supplied to a culture tank (light receiving area 200 cm ² × depth 5 cm) at a rate of 0.15 L / water volume L · min. Was replaced at a flow rate of 2 L / hr to supply dissolved nitrogen to the reactor. Also,
After preparing Aosa with a leaf punch to a size of 0.6 cm in diameter,
3,000 individuals of this Aosa were added to the culture tank, and after 4 days of preliminary culture, they were cultured for 10 days. The growth rate was determined by this culture. As a result, it was confirmed that the cells grew at 15 g (dry weight) / m ² · day. On the other hand, when the alga bodies were collected and the contents of nitrogen and phosphorus were measured, they were found to be 3% and 0.2%, respectively, based on the dry weight, indicating that nitrogen and phosphorus were absorbed from seawater.

Next, SO ₄ ²⁻ as a sulfur component was added to Aosa that had grown on day 10 of culture at a final concentration of 0.34 g / L (Mg
Final concentration with the addition of SO ₄ · 7H ₂ O in addition) 1 mg /
L (1 ppm) of hydrogen peroxide was added and cultured for 2 days.
As a result, as shown in FIG. 2, when both substances were added, the D-cysteinolic acid content increased the most as compared with those using only seawater for culture (no addition) and those added alone. However, it was found to increase by about 30%.

In the drying process of Aosa, the content of D-cysteinolic acid was measured for Aosa dried under direct sunlight and Aosa air-dried in a room protected from direct sunlight. As a result, as shown in FIG. 3, by blocking the direct sunlight and drying indoors, the decomposition of D-cysteinolic acid by sunlight can be suppressed, and the content loss of D-cysteinolic acid can be reduced. 30% can be improved.

(Example 2) Next, Aosa obtained by the above-described culture was washed with pure water, dehydrated, dehydrated, spread on newsprint, and blown in a room protected from direct sunlight. And dried. 20 g dry weight
I got Aosa.

After crushing this Aosa to a size of several mm, 60 (v / v)
% Ethanol aqueous solution and stirred at 70 ° C. for 1 hour.
The solution was concentrated under reduced pressure to about half the volume, degreased and decolorized with diethyl ether to obtain a translucent aqueous solution. Next, about 20 g of granular activated carbon was added to 1 L of the solution to adsorb excess dye, and the activated carbon was filtered with a Buchner funnel to obtain a transparent aqueous solution.

The filtrate was concentrated by an evaporator and applied to a strongly acidic cation exchanger Dowex 50W × 8 (H ⁺ type).
I got through part. This fraction was concentrated, weakly basic anion exchanger Dowex 2 × 8 ^- was applied to (OH type), after pass through, to obtain a fraction eluted with 5% acetic acid. Next, this fraction was concentrated, and further, a weakly basic anion exchanger Dowex2 ×
8 ^- using (OH type), to obtain a fraction eluted in the same 5% acetic acid. The excess acetic acid in the fraction thus obtained is removed by an evaporator, and the syrup-like liquid is dissolved in water. Then, ethanol having a final concentration of 75 (v / v)% is added, and the mixture is stored and allowed to stand under cooling. As a result, white needle-like crystals of D-cysteinolic acid were obtained.

The amount of D-cysteinolic acid obtained was 80 mg, and the yield from the raw material Aosa was 0.4%. The properties of D-cysteinolic acid thus obtained were as follows. Easily soluble in water, insoluble in ethanol. Elemental analysis: C: 23.22, H: 5.85, N: 9.03
4, a peak of a deprotonated molecular weight of 154 containing a sulfite group (molecular weight: 80) was observed from the LC-MASS analysis as shown in FIG. This confirmed that it was D-cysteinolic acid having a molecular weight of 155.

[0062]

According to the present invention, D-cysteinolic acid can be produced by culturing sterile blue seaweed on the sea using nitrogen or phosphorus in seawater as a nutrient source. In addition, sterile Aosa can be produced all year round, and has good storage stability in a dry state. Therefore, D-cysteinolic acid having a desired purity can be stably supplied as a raw material without being affected by the season. be able to. In addition, since the sterile Aosa has a D-cysteinolic acid content which is one order of magnitude higher than that of sardines, the present invention can produce D-cysteinolic acid at a 40-fold higher efficiency and economically than the conventional method. . On the other hand, since nitrogen and phosphorus in the sea area can be fixed by Aosa culture in this system, it is possible to provide a secondary effect of sea area environment purification such as purification of sea water.

[Brief description of the drawings]

FIG. 1 is a diagram showing a process for producing D-cysteinolic acid.

Fig. 2 D- by adding sulfate ion and hydrogen peroxide
The figure which shows a cysteinolic acid content improvement.

FIG. 3 is a diagram showing an improvement in D-cysteinol content by drying by indirect irradiation.

FIG. 4 is a diagram showing identification of D-cysteinolic acid by a mass spectrum.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Aosa culture | cultivation process 2 ... Aosa collection | recovery process 3 ... Sulfur compound and peroxide addition culture | cultivation process 4 ... Aosa washing | cleaning process 5 ... Aosa drying process 6 ... D-cysteinolic acid extraction process

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (reference) (C12P 13/00 C12R 1:89) F term (reference) 2B026 AA05 AB08 AC01 AF04 4B064 AE61 BA02 CA08 CC03 CD11 CE08 CE11 CE15 CE16 DA01 DA16 4B065 AA83X BB11 BB12 BD16 CA16 CA44 4C206 AA04 JA08 KA18 ZA54

Claims (7)

[Claims] In a first culture tank having an open top, D-cysteinol is supplied while supplying seawater containing nitrogen and phosphorus into the culture tank and bubbling carbon dioxide gas into the culture tank. A method for producing D-cysteinolic acid, comprising a step of culturing Aosa containing an acid, and a step of extracting D-cysteinolic acid from the Aosa with an organic solvent or hot water. 2. After culturing in the culturing step according to claim 1,
Recovering the Ulva; increasing the D-cysteinolic acid content in the Ulva by culturing in a second culture tank while adding a sulfur compound and / or peroxide to the collected Ulva; A method for producing D-cysteinolic acid, comprising the steps of: culturing so as to cause D-cysteinolic acid to be extracted from Aosa with an organic solvent or hot water. 3. A step of recovering the Ulva produced by the culture method according to claim 1 or 2, and then drying the Ulva under a light-shielded environment, and converting D-cysteinolic acid from the Ulsa into an organic solvent or heat. Extracting with water. 4. A method comprising the steps of: purifying D-cysteinolic acid obtained by the production method according to any one of claims 1 to 3 with an ion exchange resin, and crystallizing in the presence of ethanol or methanol. A method for producing D-cysteinolic acid. 5. The method according to claim 1, wherein said Aosa is a sterile Aosa.
-Method for producing cysteinolic acid. 6. A first culturing apparatus for cultivating Aosa containing D-cysteinolic acid, comprising a first culturing tank having an open top, and nitrogen and phosphorus in the culturing tank. A first culturing apparatus including a supply unit and a stirring unit for supplying seawater, and an aeration unit for bubbling carbon dioxide gas in the culturing tank; and collecting after culturing in the first culturing unit. A second culturing apparatus for culturing the above-mentioned Aosa, comprising a second culturing tank having no perforated wall, and an adding means for adding a sulfur compound and / or a peroxide. A culturing device; a drying device for drying the blue seaweed after collecting the blue seaweed produced in the first culturing device or the second culturing device, and having a structure for protecting direct sunlight; Drying room with opening and closing part of Drying apparatus and comprising a humidity means;
An apparatus for extracting D-cysteinolic acid from the seaweed treated by the apparatus according to any one of the first culture apparatus, the second culture apparatus, or the drying apparatus, wherein the organic solvent or heat is used. An apparatus for producing D-cysteinolic acid, comprising: an extraction device having means for stirring with water. 7. The production apparatus according to claim 6, further comprising a purification means using an ion exchange resin for purifying D-cysteinolic acid from the extract obtained by the extraction apparatus, and coexistence of ethanol or methanol. An apparatus for producing D-cysteinolic acid, comprising: