JP2000516094A - 試験試料から阻害物を除去する方法 - Google Patents
試験試料から阻害物を除去する方法Info
- Publication number
- JP2000516094A JP2000516094A JP10510051A JP51005198A JP2000516094A JP 2000516094 A JP2000516094 A JP 2000516094A JP 10510051 A JP10510051 A JP 10510051A JP 51005198 A JP51005198 A JP 51005198A JP 2000516094 A JP2000516094 A JP 2000516094A
- Authority
- JP
- Japan
- Prior art keywords
- test sample
- buffer
- amplification
- target sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.a)液体および標的配列を含む試験試料を提供する段階と、 b)前記試験試料を酸性化して前記標的配列を第二の液体中に置く段階と、お よび c)前記第二の液体を、核酸増幅に適する緩衝液と置き換える段階と を含む、核酸増幅検定における阻害を軽減する方法。 2.段階b)の前に、前記標的配列を塩基性リン酸塩含有溶液と接触させる、請 求の範囲第1項に記載の方法。 3.前記試験試料が臨床試験試料である請求の範囲第2項に記載の方法。 4.前記試験試料かさらにカルシウムイオンを含む、請求の範囲第1項に記載の 方法。 5.前記酸性化が、 a)前記試験試料を遠心分離して、上清およびペレットを形成させる段階と、 b)前記上清を除去する段階と、および c)前記ペレットを酸性緩衝液中に懸濁させる段階と を含む、請求の範囲第1項に記載の方法。 6.前記酸性緩衝液が、100mMと2Mの間の酢酸ナトリウムを含み、3.0 と4.5の間のpHを有する請求の範囲第5項に記載の方法。 7.a)前記試料を塩基性溶液で処理し、それによって塩基性の試験試料を形成 させる段階と、および b)前記試験試料中に含有されるいずれもの標的配列を、核酸増幅に適した緩 衝液に置く段階と を含む核酸増幅用の試験試料を調製する方法において、 改良点が、段階a)の後でありかつ段階b)の前に、前記塩基性の試験試料を 酸性化する段階を含む方法。 8.前記試験試料が臨床試験試料である請求の範囲第7項に記載の方法。 9.前記方法が、段階b)の前に前記塩基性溶液をリン酸塩含有溶液で処理する 段階をさらに含む、請求の範囲第8項に記載の方法。 10.前記酸性化が、 a)前記塩基性試験試料を遠心分離して、上清およびペレットを形成させる段 階と、 b)前記上清を除去する段階と、および c)前記ペレットを酸性緩衝液に懸濁させる段階と を含む、請求の範囲第7項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US69857396A | 1996-08-15 | 1996-08-15 | |
US08/698,573 | 1996-08-15 | ||
PCT/US1997/014380 WO1998006877A2 (en) | 1996-08-15 | 1997-08-13 | Method for removing amplification inhibitors from test samples |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2000516094A true JP2000516094A (ja) | 2000-12-05 |
JP2000516094A5 JP2000516094A5 (ja) | 2005-04-07 |
Family
ID=24805824
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10510051A Ceased JP2000516094A (ja) | 1996-08-15 | 1997-08-13 | 試験試料から阻害物を除去する方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US20040170991A1 (ja) |
EP (1) | EP0920533A2 (ja) |
JP (1) | JP2000516094A (ja) |
CA (1) | CA2262527A1 (ja) |
WO (1) | WO1998006877A2 (ja) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101935367B1 (ko) | 2012-07-26 | 2019-01-04 | 삼성전자주식회사 | 이온성 액체를 사용하여 생물학적 시료로부터 핵산 증폭의 증폭 효율 및 감도를 증가시키는 방법 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
US4874703A (en) * | 1985-08-26 | 1989-10-17 | Eli Lilly And Company | Expression vectors for use in E. coli |
US6015689A (en) * | 1993-05-24 | 2000-01-18 | Takara Shuzo Co., Ltd. | Regulation of aureobasidin sensitivity |
DE69433425T2 (de) * | 1993-08-30 | 2004-10-07 | Promega Corp | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
-
1997
- 1997-08-13 JP JP10510051A patent/JP2000516094A/ja not_active Ceased
- 1997-08-13 CA CA002262527A patent/CA2262527A1/en not_active Abandoned
- 1997-08-13 WO PCT/US1997/014380 patent/WO1998006877A2/en not_active Application Discontinuation
- 1997-08-13 EP EP97938331A patent/EP0920533A2/en not_active Withdrawn
-
2003
- 2003-06-17 US US10/463,637 patent/US20040170991A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1998006877A3 (en) | 1998-05-14 |
CA2262527A1 (en) | 1998-02-19 |
WO1998006877A2 (en) | 1998-02-19 |
EP0920533A2 (en) | 1999-06-09 |
US20040170991A1 (en) | 2004-09-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20040723 |
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A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20040723 |
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A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20070515 |
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A313 | Final decision of rejection without a dissenting response from the applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A313 Effective date: 20071003 |
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A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20071113 |