JP2000510230A - 大豆ペルオキシダーゼ電気化学センサ - Google Patents
大豆ペルオキシダーゼ電気化学センサInfo
- Publication number
- JP2000510230A JP2000510230A JP09515039A JP51503997A JP2000510230A JP 2000510230 A JP2000510230 A JP 2000510230A JP 09515039 A JP09515039 A JP 09515039A JP 51503997 A JP51503997 A JP 51503997A JP 2000510230 A JP2000510230 A JP 2000510230A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- hydrogen peroxide
- electrode
- peroxidase
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Inert Electrodes (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.電気化学過酸化水素センサであって、 非腐食性電極と、 前記非腐食性電極で固定化された耐熱性ペルオキシダーゼと、 から成り、 浸出可能な成分を有さず、37℃以上で動作可能であり、継続動作での1時間 当たりの感度の損失が2%以下であることを特徴とするセンサ。 2.前記耐熱性ペルオキシダーゼは大豆から得られることを特徴とする請求項1 に記載の過酸化水素センサ。 3.前記ペルオキシダーゼは、レドックスヒドロゲルを介して前記電極で固定化 されることを特徴とする請求項1に記載の過酸化水素センサ。 4.前記ペルオキシダーゼは、レドックスポリマーと架橋することを特徴とする 請求項1に記載の過酸化水素センサ。 5.前記ペルオキシダーゼは、核酸或はオリゴヌクレオチドと共有結合すること を特徴とする請求項1に記載の過酸化水素センサ。 6.前記ペルオキシダーゼは、再結合型タンパク質であることを特徴とする請求 項1に記載の過酸化水素センサ。 7.前記センサは、更に過酸化水素の生成を触媒する酵素からなることを特徴と する請求項1に記載の過酸化水素センサ。 8.前記酵素は、グルコースオキシダーゼと、ラクタートオキシダーゼと、コレ ステロールオキシダーゼとからなるグループから選択されることを特徴とする請 求項7に記載の過酸化水素センサ。 9.前記センサは更に、前記電極の少なくとも一部を被覆し、過酸化水素を透過 させる生体適合性ポリマーコーティングからなることを特徴とする請求項1に記 載の過酸化水素センサ。 10.前記過酸化水素センサは、少なくとも50℃の温度で動作することができ 、継続動作の1時間当たりの感度の損失が5%以下であることを特徴とする請求 項1に記載の過酸化水素センサ。 11.過酸化水素の電気化学分析方法であって、 過酸化水素の存在下において、非腐食性電極で浸出不可能なレドックス化合物 と相互固定化される耐熱性ペルオキシダーゼからなる電極によって電気信号を発 生させるステップと、 前記発生した電気信号と過酸化水素の存在とを過酸化水素のサンプル内にて関 係づけるステップと、 からなる方法。 12.分析物の測定を継続して行う方法であって、 非腐食性電極で浸出不可能なレドックス化合物と相互固定化される耐熱性ペル オキシダーゼと、前記電極で固定化されており、特定の分析物の存在下において 過酸化水素を発生させる反応を触媒する第2酵素と、からなるセンサを、身体流 体若しくは組織の上又は中に配置するステップと、 前記電極によって発生した電気信号と前記身体流体或は組織内にある前記分析 物の量とを関係づけるステップと、 からなる方法。 13.生体内でグルコース濃度を観測する方法であって、 非腐食性電極で浸出不可能なレドックス化合物と相互固定化される耐熱性ペル オキシダーゼと、前記電極で固定化されるグルコースオキシダーゼと、からなる センサを被験者の身体流体或は組織内に配置するステップと、 前記電極によって発生した電気信号と、前記身体流体或は組織内にあるグルコ ースの量とを関係づけるステップと、 からなる方法。 14.37℃以上で動作するバイオリアクター内の分析物の濃度を観測する方法 であって、 非腐食性電極で浸出不可能なレドックス化合物と相互固定化される耐熱性ペル オキシダーゼからなるセンサを、前記バイオリアクター内に前記バイオリアクタ ーの内容物と接触させて、配置するステップと、 前記電極によって発生する電気信号と、前記バイオリアクター内の前記分析物 とを関係づけるステップと、 からなる方法。 15.核酸プローブとサンプルの核酸とのハイブリッド形成を検出する方法であ って、 非腐食性電極でサンプルの核酸を固定化するステップと、 前記サンプル核酸電極を耐熱性ペルオキシダーゼと結合した核酸プローブとと もにハイブリッド形成条件下に置くステップと、 添加された過酸化水素の存在下で前記電極によって発生した電気信号と、前記 サンプルの核酸と前記核酸プローブとのハイブリッド形成とを関係づけるステッ プと、 からなる方法。 16.前記方法は更に、前記電極と前記耐熱性ペルオキシダーゼ核酸プローブと の電気的な接触を分断するステップを含むことを特徴とする請求項15に記載の 方法。 17.前記分断するステップは、前記サンプル核酸電極と耐熱性ペルオキシダー ゼ核酸プローブとのハイブリッド形成体を変性するステップであることを特徴と する請求項16に記載の方法。 18.前記変性ステップは、前記サンプル核酸電極と前記耐熱性ペルオキシダー ゼ核酸プローブとが置かれている条件を変化させることによって起こるステップ であることを特徴とする請求項17に記載の方法。 19.サンプルの核酸の配列の組成物を分析する方法であって、 非腐食性電極でサンプルの核酸を固定化するステップと、 前記サンプル核酸電極を、ハイブリッド形成条件の下に、耐熱性ペルオキシダ ーゼと結合した核酸プローブとともに置くステップと、 核酸プローブと、前記サンプルの核酸及び前記サンプルの核酸配列の組成物と のハイブリッド形成、並びに添加された過酸化水素の存在下において、前記電極 によって発生する電気信号を関係づけるステップと、 からなる方法。 20.サンプルの核酸の配列の組成物を分析する方法であって、 非腐食性電極にサンプルの核酸を固定化するステップと、 前記サンプル核酸電極を、変化するハイブリッド形成条件下に、耐熱性ペルオ キシダーゼと結合した核酸プローブとともに置くステップと、 核酸プローブと、前記サンプルの核酸及び前記サンプルの核酸配列の組成物と のハイブリッド形成の変化、並びに前記変化するハイブリッド形成条件下の添加 された過酸化水素の存在下において、電極によって生成される電気信号の変化を 関係づけるステップと、 からなる方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/540,789 US5665222A (en) | 1995-10-11 | 1995-10-11 | Soybean peroxidase electrochemical sensor |
US08/540,789 | 1995-10-11 | ||
PCT/US1996/014534 WO1997013870A1 (en) | 1995-10-11 | 1996-09-11 | Soybean peroxidase electrochemical sensor |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2000510230A true JP2000510230A (ja) | 2000-08-08 |
Family
ID=24156947
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP09515039A Ceased JP2000510230A (ja) | 1995-10-11 | 1996-09-11 | 大豆ペルオキシダーゼ電気化学センサ |
Country Status (9)
Country | Link |
---|---|
US (1) | US5665222A (ja) |
EP (1) | EP0856064B1 (ja) |
JP (1) | JP2000510230A (ja) |
AT (1) | ATE219147T1 (ja) |
AU (1) | AU7017896A (ja) |
DE (1) | DE69621814T2 (ja) |
DK (1) | DK0856064T3 (ja) |
ES (1) | ES2178715T3 (ja) |
WO (1) | WO1997013870A1 (ja) |
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