JP2000327525A - Skin preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin preparation for external use, having excellent activities for preventing the skin from being aged, and capable of imparting tenseness and gloss to the skin by including an extract of Myrciaria dubia, and a cell- activating agent. SOLUTION: This skin preparation for external use contains an extract of Myrciaria dubia, and a cell-activating agent. The content of the cell-activating agent is 0.0001-5 wt.% expressed in terms of dried solid, and the content of the included extract of the Myrciaria dubia is 0.0001-5 wt.% expressed in terms of the dried solid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin, and more particularly, to an excellent preparation for preventing skin aging and imparting a shine to the skin by containing a cam cam extract and a cell activator. It relates to a skin external preparation.

[0002]

2. Description of the Related Art Conventionally, skin external preparations such as emulsions, creams, lotions, packs, dispersions, detergents, ointments, dispersions, and external preparations have been used to prevent aging of the skin and give the skin a luster. For that purpose, medicinal ingredients such as cell activators have been added. For example, for the purpose of skin aging prevention, cell activation,
Pharmaceutical ingredients such as carrot extract, bovine blood deproteinizing extract, and bifidobacterium fermentation metabolite are incorporated.

[0003]

However, external preparations for skin containing these cell activators do not have sufficient effects of the medicinal components or, if they are added in a sufficient amount to obtain the medicinal effects, the stability will be degraded. Improvements such as chipping or impaired usability have been desired.

[0004]

Means for Solving the Problems The present inventors have made intensive studies to improve the effect of a cell activator added to an external preparation for the purpose of preventing aging and giving the skin a shine. As a result, it has been found that when the camcam extract is combined with the cell activator, the action originally possessed by the cell activator is sufficiently exhibited, and the present invention has been completed. That is, the present invention provides a skin external preparation containing a camcam extract and a cell activator.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The cam cam extract, which is the component (A) of the present invention, is obtained from the fruit of a cam cam (scientific name Myrcilia Dubia), a plant belonging to the family Myrtaceae.
Extract using an extraction solvent. The preparation method is not particularly limited, but, for example, extraction is performed at low temperature or at room temperature to heating using various suitable solvents.

As the extraction solvent, for example, one or more of water; lower monohydric alcohols such as methyl alcohol and ethyl alcohol; and liquid polyhydric alcohols such as glycerin, propylene glycol and 1,3-butylene glycol are used. be able to. As an example of a preferred extraction method, extraction is performed at a low temperature for 2 to 3 days using ethyl alcohol having a water content of 20 to 90% (v / v), followed by filtration, and the obtained filtrate is left for about 5 days. And then aging and filtering again.

The content of the cam cam extract, which is the component (A) of the present invention, is 0.0001 to 5% by weight as dry solids.
(Hereinafter simply referred to as “%”), more preferably 0.001 to 3%. When the content of the camu cam extract is in this range, the effects of the present invention are better exhibited.

The cell activator which is the component (B) of the present invention is preferably as follows.

Preferred cell activators include grape seed oil, deoxyribonucleic acid and its derivatives, adenylic acid derivatives such as adenosine triphosphate and adenosine monophosphate, ribonucleic acid and its derivatives, cyclic AMP, cyclic GMP, Flavin adenine dinucleotide,
Vitamin A such as carotenoids such as guanine, adenine, cytosine, thymine, xanthine and derivatives thereof, retinol and derivatives thereof (retinol palmitate, retinol acetate, etc.), retinal and derivatives thereof, dehydroretinal and carotene; thiamines (thiamine hydrochloride salt,
Thiamine sulfate), riboflavins (riboflavin, riboflavin acetate, etc.), pyridoxines (pyridoxine hydrochloride, pyridoxine dioctanoate, etc.), cyanocobalamins, folic acids, nicotinic acids (nicotinamide, benzyl nicotinate, etc.), cholines, etc. Vitamin Cs such as vitamin Bs, ascorbic acid and its derivatives, α- and γ-linolenic acid and their derivatives, eicosapentaenoic acid and its derivatives, estradiol and its derivatives, glycolic acid, succinic acid, lactic acid, salicylic acid, etc. And one or more of these can be appropriately selected and added. In addition,
These “derivatives” include salts that can be formed.

Among these cell activators, particularly preferred are adenosine triphosphate and its derivatives, estradiol and its derivatives, retinal and its derivatives, retinol palmitate, lactic acid, succinic acid and their derivatives.

Other preferred cell activators include placental extracts such as pigs and cows, calf blood extracts, serum deproteinized extracts, spleen extracts, egg components such as birds, cockscomb extracts, and shells. Extracts, shellfish extracts, royal jelly, silk protein and its degradation products or their derivatives, hemoglobin or its degradation products, lactoferrin or its degradation products, mollusc extracts such as squid, fish meat extracts, yeast,
Fermentation metabolites such as lactic acid bacteria, bifidobacteria, asparagus extract, apricot extract, ginkgo extract, barley extract, oak extract, orange extract, cucumber extract, kiwi extract, shiitake extract, and horsetail extract , Assembly extract, soybean extract, syrup extract, calendula extract, capsicum extract, garlic extract,
Carrot extract, liquorice extract, beech bud extract,
Peach extract, lettuce extract, lemon extract, eucalyptus extract, lily bulb extract, rosemary extract, hinokitiol, cepharanthin, ganoderma extract and the like, and one or more of these may be appropriately selected. Can be added.

Among these cell activators, particularly preferred are placenta extract, shellfish extract, carrot extract, lactic acid bacteria fermentation metabolite, ginkgo extract, barley extract, oak extract, soybean extract, Beech bud extract.

The content of the cell activator (B) in the present invention is preferably 0.0001 to 5%, more preferably 0.001 to 3%. The content of the extract is 0.0001 to 5%, preferably 0.001 to 3%, as a dry solid content. When the extract is used as it is, as long as it is in this range as a dry solid content, when combined with the camcam extract, it does not affect the formulation and the camcam extract in the formulation, and has good stability over time and is excellent. An external preparation for skin that exhibits a cell activating effect and has an excellent feeling upon use can be obtained.

The external preparation for skin of the present invention can be prepared according to a conventional method from the essential components (A) and (B) into various forms known as ordinary external preparations for skin.

The form of the external preparation for skin is not particularly limited, and examples thereof include skin care cosmetics such as milky lotions, creams, lotions, packs, cleaning agents, makeup cosmetics such as lipsticks and foundations, cosmetics for the scalp, and the like. Ointments, dispersions, pharmaceuticals such as topical liquids and the like can be used, and there is no particular limitation on the form of the preparation. Solid, paste, mousse,
Gel, powder, solution, solubilizing, emulsifying, powder dispersing and multi-layered can be used.

In addition to the components (A) and (B), the external preparation for skin of the present invention usually contains cosmetics, quasi-drugs, and external medicines as long as the effects of the present invention are not impaired. Ingredients used in the formulation, i.e., water, oils, surfactants, metal soaps, gelling agents, powders, alcohols, water-soluble polymers, film-forming agents, resins, UV protection agents, clathrate compounds, antibacterial Agents, fragrances, deodorants, salts, pH regulators, cooling agents, animal / microbial extracts, plant extracts, blood circulation enhancers, astringents, antiseborrheic agents, active oxygen scavengers, whitening agents, anti-inflammatory Agents, keratolytic agents, enzymes, hormones, vitamins and the like.

[0017]

EXAMPLES Next, the present invention will be described in more detail with reference to Reference Examples, Test Examples, Examples and Comparative Examples, but the present invention is not limited thereto.

REFERENCE EXAMPLE 1 Production of cam cam extract A 30 g portion of dried cam cam (Myrciaria Dubia) fruit was dried at a water content of 80% (v / v).
After adding 100 ml of ethyl alcohol and performing extraction at room temperature for 3 days, the resultant was filtered to obtain a cam cam extract (dry solid content: 3.0%).

REFERENCE EXAMPLE 2 Production of carrot extract Carrot root (250 g) was finely cut, 1 liter of absolute ethanol was added thereto, and the mixture was stirred and extracted for a day and night using a blender to obtain a carrot extract (dry solid content: 1.1%). ).

Reference Example 3 Production of placenta extract 1 kg of placenta of a cow of 3-4 months of pregnancy was frozen, thawed, and impurities were washed and removed, and 5 liters of purified water was added. Filtration gave a placenta extract (3.5% dry solids).

Reference Example 4 Production of Fermentation Product of Lactic Acid Bacteria 1% of a milk protein aqueous solution was inoculated with 1% of a lactic acid bacterium and cultured at 40 ° C. for 3 days. 80 of the obtained fermented milk
The solution was flash sterilized at ℃ and filtered to obtain a lactic acid bacteria fermentation product (dry solid content: 9.5%).

REFERENCE EXAMPLE 5 Production of oak extract
Then, the mixture was extracted at room temperature for 3 days with occasional stirring, and filtered to obtain an oak extract (dry solid content: 1.0%).

Test Example 1 Fibroblast Proliferation Test An appropriate amount of medium was taken in a petri dish, and human neonatal fibroblasts were inoculated and allowed to stand at 37 ° C. in a carbon dioxide concentration of 5%.
On the next day, each of the freeze-dried cell activators shown in Table 1 was used at a concentration of 1%.
0 mg / ml, and the camcam extract was dissolved in PBS (phosphate buffer) to 100 mg / ml, and 1/100 of the medium.
Add 10 volumes to a Petri dish and mix. As a control, PBS
Is added. The number of cells (A) and the number of control cells (B) for each sample were measured, and the cell growth rate was calculated according to Equation 1. Table 1 shows the above results.

[0024]

(Equation 1)

[0025]

[Table 1]

As is evident from the results in Table 1, a very high cell proliferation promoting effect was obtained by combining the cam cam extract with the carrot extract and the placenta extract as cell activators.

Examples 1 to 3 and Comparative Examples 1 to 4: Emulsions Emulsions having the compositions shown in Table 2 were prepared, and the effects of preventing skin aging were examined. Table 2 also shows the results.

(Composition and results)

[Table 2]

(Production method) A part of the component (14) and (6) and (11) are mixed by heating and maintained at 70 ° C. B. The components (1) to (5) are mixed by heating and kept at 70 ° C. C. Add B to A, emulsify uniformly, cool to 30 ° C., add components (10), (12) and (13) and mix uniformly. D. The components (7) to (9) were mixed with the residue of the component (14), added to C, and uniformly mixed to obtain an emulsion.

(Test method) 26 to 5 per test emulsion
A panel of 15 0-year-old women, twice daily, morning and night,
After washing the face for 12 weeks, an appropriate amount of the test emulsion was applied to the face. The skin aging prevention effect of the application was evaluated according to the following criteria.

Skin aging prevention effect: [Evaluation] [Contents] Effective Skin peeling and gloss were improved. Slightly effective Skin glue, gloss slightly improved. Ineffective No change from before use. The results obtained by the above evaluation method are also shown in Table 2.

As is evident from the results in Table 2, the emulsion containing the camcam extract and the cell activator according to the present invention is applied to the skin to improve the skin shine and gloss and to age the skin. It was found to prevent

Embodiment 4 FIG. Lotion (Formulation) (%) (1) Glycerin 5.0 (2) 1,3-butylene glycol 6.5 (3) Polyoxyethylene (20EO) Sorbitan monolaurate 1.2 (4) Ethyl Alcohol 8.0 (5) Kamcum extract * 1 1.0 (6) Barley extract * 2 1.0 (7) Oat extract * 3 1.0 (8) Preservatives appropriate amount (9) Flavors appropriate amount (10 ) Alkaline simple hot spring water * 4 50.0 (11) Remaining purified water * 1 Manufactured in Reference Example 1 * 2 Manufactured by Sansho Pharmaceutical * 3 Manufactured in Reference Example 5 * 4 Manufactured by Maruzen Pharmaceutical

(Production method) A. Components (3), (4), (8) and (9) are mixed and dissolved. B. Components (1), (2), (5), (6), (7),
(10) and (11) are mixed and dissolved. C. A and B were mixed and made uniform to obtain a lotion.

Example 4 was a lotion having excellent stability over time and, when applied to the skin, improved skin abrasion and luster and had an effect of preventing skin aging.

Embodiment 5 Ointment (Prescription) (%) (1) Stearic acid 18.0 (2) Cetanol 4.0 (3) Triethanolamine 2.0 (4) Glycerin 5.0 (5) Camcam extract * 1 10.0 ( 6) Ginkgo extract * 2 1.0 (7) Placenta extract * 3 1.0 (8) Lactic acid * 4 1.0 (9) Purified water balance * 1 Produced in Reference Example 1 * 2 Tokiwa plant Manufactured by Chemical Research Laboratory * 3 Manufactured in Reference Example 3 * 4 Manufactured by Daiichi Pharmaceutical

(Production method) A. A part of components (3), (4) and (9) are mixed by heating and kept at 75 ° C. B. Heat mix components (1) and (2) and maintain at 75 ° C. C. Add A slowly to B. D. C was dissolved in the remainder of (9) while cooling (5)-
(8) was added to obtain an ointment.

Example 5 was an ointment having an excellent cell activating effect.

Embodiment 6 Pack (Formulation) (%) (1) Polyvinyl alcohol 20.0 (2) Ethanol 20.0 (3) Glycerin 5.0 (4) Kaolin 6.0 (5) Camcam extract * 1 2.0 (6) Soybean extract * 2 3.0 (7) Shellfish extract * 3 1.0 (8) Suitable amount of preservative (9) Suitable amount of flavor (10) Remaining amount of purified water * 1 Produced in Reference Example 1 * 2 Maruzen * 3 Maruzen Pharmaceutical

(Production method) A. Mix components (1), (3), (4) and (10), heat to 70 ° C. and stir. B. Mix components (2), (8) and (9). C. B is added to A, mixed and cooled (5),
(6) and (7) were uniformly dispersed to obtain a pack.

Example 6 was a pack having an excellent cell activating effect and imparting elasticity to the skin.

Embodiment 7 Detergent (Prescription) (%) (1) Stearic acid 10.0 (2) Palmitic acid 8.0 (3) Myristic acid 12.0 (4) Lauric acid 4.0 (5) Oleyl alcohol 1.5 (6) ) Purified lanolin 1.0 (7) Perfume proper amount (8) Preservative proper amount (9) Glycerin 18.0 (10) Potassium hydroxide 6.0 (11) Camcam extract * 1 0.5 (12) Beech bud Extract * 2 5.0 (13) Remaining amount of purified water * 1 Manufactured in Reference Example 1 * 2 Manufactured by GATTEFOSSE

(Production method) Mix components (9), (10) and (13) and mix
Heat to ° C. B. Mix components (1)-(6) and (8) and heat to 70 ° C. C. B is added to A, kept at 70 ° C. for a while,
After cooling to 0 ° C., components (7), (11) and (12) were added, and the mixture was cooled to obtain a detergent.

Example 7 was a cleaning agent for imparting a shine and shine to the washed skin.

Embodiment 8 FIG. Lipstick (Prescription) (%) (1) Camcam extract * 1 0.02 (2) γ-linolenic acid * 2 0.02 (3) Lactic acid bacteria fermentation metabolite * 3 0.01 (4) Red 202 0.2 (5) Candelilla wax 9.0 (6) Solid paraffin 8.0 (7) Beeswax 5.0 (8) Carnauba wax 5.0 (9) Lanolin 11.0 (10) Isopropyl myristate 10.0 (11) Retinol palmitate * 4 1.0 (12) Refined castor oil Remaining (13) Perfume suitable amount * 1 Manufactured in Reference Example 1 * 2 Wako Pure Chemical Industries * 3 Manufactured in Reference Example 4 * 4 Nippon Roche Company

(Production method) After heating and dissolving the components (5) to (10) and (12),
Knead with a roller. B. In A, components (1) to (3) and (4), (11),
After adding (13), knead again with a roller. C. B was cooled and molded to obtain a lipstick.

Example 8 was a lipstick that gave a good gloss and a lip to the lips.

[0048]

As described above, by combining the cam cam extract with the cell activator, the original effect of the cell activator can be sufficiently exhibited. That is, since it has a stable and excellent anti-aging effect on the skin, it maintains the abrasion and luster of the skin and exerts a high inhibitory effect on wrinkle formation. Therefore, since the skin external preparation of the present invention can sufficiently exhibit the inherent effects of the cell activator, it is extremely useful in beauty and medicine.

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Claims (6)

[Claims] 1. An external preparation for skin comprising the following components (A) and (B): (A) a camcam extract; and (B) a cell activator. 2. The cell activator is grape seed oil, deoxyribonucleic acid and its derivatives, adenylic acid derivatives, ribonucleic acids and its derivatives, cyclic AMP, cyclic G
MP, flavin adenine dinucleotide, guanine, adenine, cytosine, thymine, xanthine and its derivatives, vitamin A and its derivatives, vitamin B and its derivatives, vitamin C and its derivatives, α- and γ-linolenic acid and their derivatives, 2. An organic acid selected from eicosapentaenoic acid and its derivative, estradiol and its derivative, glycolic acid, succinic acid, lactic acid, and salicylic acid, and a derivative thereof.
The topical skin preparation according to the above. 3. The cell activator content is from 0.0001 to 0.0001.
The external preparation for skin according to claim 2, which is 5% by weight. 4. The cell activator is a placenta extract, a calf blood extract, a serum deproteinized extract, a spleen extract, an egg component, a cockscomb extract, a shell extract, a shell meat extract, a royal jelly, a silk protein. And its decomposition products or derivatives thereof, hemoglobin or its decomposition products, lactoferrin or its decomposition products, mollusc extracts, fish meat extracts, fermentation metabolites, asparagus extracts, apricot extracts, ginkgo extracts, barley extracts , Oak extract, orange extract, cucumber extract, kiwi extract, shiitake extract, horsetail extract, assembly extract, soybean extract, syrup extract,
Calendula extract, Capsicum extract, Garlic extract, Carrot extract, Bulberry extract, Beech bud extract, Peach extract, Lettuce extract, Lemon extract, Eucalyptus extract, Lily bulb extract, Rosemary The external preparation for skin according to claim 1, which is selected from an extract, hinokitiol, cepharanthin, and a reishi extract. 5. The external preparation for skin according to claim 4, wherein the content of the cell activator is 0.0001 to 5% by weight as a dry solid content. 6. The skin external preparation according to any one of claims 1 to 5, wherein the content of the camcam extract is 0.0001 to 5% by weight as a dry solid content.