JP2000154133A - Cosmetic composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a cosmetic composition derived from plants, high in safety, having excellent moisture-keeping effect and bleaching effect, and useful for suppressing or treating the spots and freckles, and dyspigmentation such as darkness by adding Oenanthe storonifra, DC and/or its extract. SOLUTION: Oenanthe storonifra, DC and/or its extract is added in an amount preferably of 0.001-20 wt.%, more preferably of 0.01-5 wt.% based on the total composition. In the above-mentioned Oenanthe storonifra, the whole plant can be used, but roots are especially preferable. Examples of the extraction solvent used in the extraction include water. The composition has an anti- tyrosinase activity, and it is useful as a medicinal cosmetic aiming the prevention of inflammation caused by sun-burning and the recovery of the sun-burnt skin or the like, or a medicine for the treatment of a dermatitis caused by sun burning, or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition, which has a moisturizing effect to moisturize the skin and exhibits an excellent whitening effect by having an anti-tyrosinase activity. , Used to control or treat pigmentation such as spots, freckles, and black and white
An object of the present invention is to provide a cosmetic composition capable of providing fair and beautiful skin.

[0002]

2. Description of the Related Art Various components are blended in cosmetics according to the purpose. For example, moisturizers have been incorporated into many cosmetics in order to moisturize dry skin and maintain freshness. That is, if the moisturizing property of the skin decreases due to aging or other causes, the skin becomes dry and loses the flexibility and elasticity of the skin, and as a result, the skin may become rough or wrinkles may occur. Moisturizers are included to prevent and maintain healthy skin. As the humectant, conventionally, glycerin, propylene glycol,
1,3-butylene glycol, polyhydric alcohol, pyrrolidone carboxylic acid, saccharides, amino acids, collagen and the like have been used.

[0003] A whitening agent is also one of the components blended in many cosmetics. Whitening agents are formulated to improve, alleviate or prevent pigmentation such as spots, freckles, and black and white, and suppress the activity of tyrosinase, causing melanin pigment, which causes pigmentation, to spread on the skin. It has the function of suppressing deposition. Recently, since many people prefer fair skin, many cosmetics, such as milky lotions for whitening and lotions for whitening, have been created which claim the effect of whitening agents. As this whitening agent, ascorbic acid,
Kojic acid, arbutin, glutathione, sulfur preparations and the like have been used.

[0004]

However, moisturizing agents and whitening agents conventionally used have the following problems. That is, the moisturizing agents conventionally used have the problem that the skin damage due to the compounding is increased and the safety to the skin is low. For example, glycerin has not only a moisturizing ability but also a hygroscopic ability, and thus has a disadvantage that water in the stratum corneum is deprived, causing skin inflammation and darkening of the entire skin. on the other hand,
Conventionally used whitening agents are not satisfactory in their whitening effect, and glutathione and sulfur preparations have a peculiar off-odor, and may cause precipitation when mixed with water-soluble cosmetics. However, it cannot be used as a general additive for cosmetics.

[0005] Therefore, it is desired to create a moisturizer having a high moisturizing property and excellent safety and a whitening agent having a high whitening effect and which can be widely used as an additive for cosmetics. After continuing intensive research on moisturizers and whitening agents that are highly safe and derived from
the storonifra, DC) has both a moisturizing effect and a whitening effect, and the present invention has been completed.

[0006]

Means for Solving the Problems The invention according to claim 1 relates to a cosmetic composition characterized by containing an Apiaceae sperm (Oenanthe storonifra, DC) and / or an extract thereof.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The cosmetic composition according to the present invention comprises:
Includes Apiaceae Seri (Oenanthe storonifra, DC). The Apiaceae Seri (Oenanthe storonifra, DC) is a perennial plant that is native to Japan and has a height of 20 to 40 cm. Seri (Oen
anthe storonifra, DC) has been used as an edible plant since ancient times. In particular, it is known that its leaves are rich in essential oil components, vitamin A, vitamin C, calcium and the like. In addition, the following formula 1
And that foods containing this 6,7-hydroxy-coumarin are effective in improving allergic symptoms such as hay fever and asthma. The inventors have learned experimentally.

Embedded image

[0008] In the present invention, in the present invention,
oronifra, DC) can be used in all parts of the above-ground part and underground part. The whole plant may be used, or each part such as a leaf part or a root part may be used singly or in an appropriate mixture. In particular, when the root is used, the above-mentioned 6,7-hydroxy-coumarin contained in the root has an antiallergic activity. An improvement effect can also be exhibited, which is preferable.

The form of use of the above-mentioned seri (Oenanthe storonifra, DC) is not particularly limited, and ground or dried powder may be used as it is, or an extract thereof may be used. It is also possible to use a mixture of these.

[0010] The extraction solvent used for obtaining the extract is not particularly limited, but may be a hydrophilic organic solvent such as water or alcohol, or a mixed solution thereof, such as seri (Oenanthe storonifra, D
Any solvent can be suitably used as long as the active ingredient of C) can be suitably extracted. In addition, as a hydrophilic organic solvent, monohydric alcohols, such as methanol and ethanol, polyhydric alcohols, acetone, etc. can be illustrated. Examples of the extraction method include a cold immersion method and a hot immersion method, but are not particularly limited.

The extract obtained by the above method may be used as it is, but it can be concentrated and used as a soft extract, or dried by freeze-drying or spray-drying and used as a dry extract.

Seri (Oenanthe storonif) in a cosmetic composition
ra, DC) is not particularly limited, but as a dry solid content of the plant (in the case of an extract, the dry solid content of the plant used for extraction), 0.001 to 20% by weight of the total composition, more preferably The content is desirably 0.01 to 5% by weight.

[0013] The cosmetic composition having the above constitution has a moisturizing effect and an anti-tyrosinase activity, as is clear from the test examples described later. Therefore, the moisturizing effect gives the skin moisturizing and fresh skin can be maintained, and its anti-tyrosinase activity exerts an excellent whitening effect, so that pigmentation diseases such as spots, freckles, and black and white It can be used to suppress or treat skin and make fair and beautiful skin.

The dosage form of the cosmetic composition according to the present invention is not particularly limited, and in addition to ordinary cosmetics, its anti-tyrosinase activity is used to make it possible to use the sunburn to cause spots such as spots, freckles, and dark spots due to sunburn. It can be used as a medicated cosmetic (quasi-drug) having a specific purpose of use such as prevention of inflammation and recovery of skin after sunburn, and a drug for treating skin inflammation caused by sunburn. Specifically, lotion, cream,
It can be used as cosmetics such as emulsions, foundations, oily cosmetics, packs, skin cleansers and the like, and medicated cosmetics (quasi-drugs), ointments, liniments, lotions and the like.

In the present invention, the above-mentioned seric (Oen)
anthe storonifra, DC) and / or its extracts, other than the components that are blended into ordinary cosmetic compositions, such as oils, surfactants, ultraviolet absorbers, medicinal ingredients, alcohols, preservatives, pigments, fragrances, etc. Can be appropriately compounded within a range that does not impair the effects of the present invention.

[0016]

EXAMPLES Hereinafter, the present invention will be described based on Examples and Comparative Examples.
This will be described in detail. However, the present invention is not limited to these examples. (Example 1) Whole grass 10 of seri (Oenanthe storonifra, DC)
0 g was immersed in warm water at 60 ° C. for about 5 hours and filtered. After repeating this several times, the obtained hot water extracts were combined, concentrated under reduced pressure, and filtered again to obtain 1,000 cc of a crude extract.
The sample of Example 1 was used.

(Example 2) Seri (Oenanthe storonifra, D)
Using 100 g of the root of C), hot water extraction was performed in the same manner as in Example 1,
The obtained crude extract was used as a sample of Example 2.

Examples 3 and 4 Oenanthe storonif
ra.DC) was dispersed in 1000 cc of water to obtain a sample of Example 3. Similarly, Seri (Oenan
100 g of dry powder of the storonifra, DC) was dispersed in water to obtain a sample of Example 4.

(Comparative Examples 1 and 2) The standard product of glycerin was Comparative Example 1 and the standard product of arbutin was Comparative Example 2.

[0020]

Test Examples The following tests were performed using the samples of Examples 1 to 4 and Comparative Examples 1 and 2. (Test Example 1; Moisturizing Effect Test) Using the samples of Examples 1 to 4 and Comparative Example 1 as humectants, five kinds of lotions were prepared according to the following composition. Composition Blended amount (% by weight) Humectant 20.0 Ethanol 7.0 1,3-butylene glycol 6.0 Citric acid 0.2 Sodium citrate 0.8 Methyl parahydroxybenzoate 0.2 Ethyl paraoxybenzoate 0. 02 Appropriate amount of fragrance purified water Total remaining 100.0

Forty women with dry skin were panelists and divided into four groups of ten. Each of the four groups was a group using the lotion of Examples 1 to 4, and the left hand was applied twice a day in the morning and evening to the lotion for three weeks. In addition, the lotion of Comparative Example 1 was similarly applied to the right hand of all the panelists. After three weeks, the degree of improvement of dry skin by using the lotion of Examples 1 to 4 was evaluated by comparing the left hand and the right hand with the following four grades. ◎: Dry skin is considerably improved compared to right hand ○: Dry skin is slightly improved compared to right hand △: Dry skin is hardly improved ×: Degree of dryness is worse

The results are shown in Table 1. The numbers in the table are
◎, ○, △, × are the corresponding numbers of persons.

[Table 1]

(Test Example 2; Moisturizing Effect Test)
Using the samples of Comparative Example 4 and Comparative Example 1 as moisturizers, five kinds of creams were prepared according to the following composition. Composition Blended amount (% by weight) Humectant 20.0 Ethanol 7.0 1,3-butylene glycol 6.0 Citric acid 0.2 Sodium citrate 0.8 Methyl parahydroxybenzoate 0.2 Ethyl paraoxybenzoate 0. 02 Appropriate amount of fragrance purified water Total remaining 100.0

Women with dry skin and rough skin 25
Names were panelists and divided into five groups of five.
Five groups were used for Examples 1 to 4 and Comparative Example 1, respectively.
And used the cream on their face twice a morning and evening for three weeks. Three weeks later, the degree of improvement in skin roughness and moist feeling were evaluated on the following four scales. Skin roughening improvement effect ◎: considerably improved compared to before use ○: slightly improved compared to before use △: almost unchanged from before use ×: moist feeling worse than before use ◎ used Very moist compared to before ○: Slightly moist compared to before use △: Almost the same as before use ×: Deteriorated compared to before use

The results are shown in Table 2. The numbers in the table are
◎, ○, △, × are the corresponding numbers of persons.

[Table 2]

(Test Example 3; Measurement of anti-tyrosinase activity)
B16 melanoma 4 cultured in 10% FBS-DMEM
A5 (RIKEN Cell Bank) in a 96-well plate, 5 × 10 ⁴ cells
s / well (total 195 μl), 37
Culturing was performed for 24 hours at 5 ° C. and 5% CO ₂ . Here, the sample solutions of Examples 1 to 4 and Comparative Example 2 were diluted with a 50% ethanol / PBS solution so as to have final concentrations of 5, 10, 50, 100, and 500 ppm, respectively.
5 μl each of which was sterilized by filtration through a 2 μm membrane filter was added, and the cells were further cultured under the same conditions for 24 hours. After the culture, the culture was removed and 1% Triton X-10 was removed.
After solubilizing the cell membrane with 50 μl of 0 / PBS solution, 100 μl of 10 mM L-DOPA was added as a substrate,
The reaction was performed at 37 ° C. for 3 hours, and the absorbance at a wavelength of 475 nm was measured using a microplate reader. Further, as a control, the absorbance of the reaction system in the case where the sample solution was not added was measured, and the tyrosinase biosynthesis inhibition rate (%) was calculated based on the following equation 1 (Equation 1).

(Equation 1) A: Absorbance without sample solution added B: Absorbance with sample solution added

The results are shown in Table 3.

[Table 3]

From the results of Tables 1 to 3, it is found that
oronifra, DC) or a cosmetic composition containing the extract thereof has a high moisturizing effect and also has an anti-tyrosinase activity. Therefore, the cosmetic composition according to the present invention can provide moisture to the skin with excellent moisturizing properties, exerts an excellent whitening effect due to anti-tyrosinase activity, and is effectively used as a cosmetic for whitening. It can also be used for suppressing or treating pigmentation diseases such as spots, freckles, and black and white.

(Test Example 4; Antiallergic test) Using the samples of Examples 2 and 4 as a humectant, a lotion was prepared with the same composition as in Test Example 1, and using this lotion, Oenanthe storonifra, DC) was tested for antiallergic properties of roots. Women with allergic dermatitis 20
The names were panelists and divided into two groups of ten. Each of the two groups was a group using the lotion of Example 2 or 4, and the face was applied with the lotion twice in the morning and evening for three weeks. Three weeks later, the degree of improvement in symptoms was evaluated on the following four scales. ◎: considerably improved compared to before use ○: slightly improved compared to before use △: almost unchanged from before use ×: worse than before use

The results are shown in Table 4.

[Table 4] From the results in Table 4, it can be seen that the cosmetic composition containing the root of Seri (Oenanthe storonifra, DC) or an extract thereof also has antiallergic properties and is also effective in improving allergic dermatitis and the like. Can be used.

Hereinafter, the formulation examples of the present invention are shown. (Formulation Example 1: Emulsion) Composition Blended amount (% by weight) Extract of Example 1 15.0 Squalane 5.0 Vaseline 2.0 Sorbitan sesquioleate 0.8 Polyoxyethylene oleyl ether (20E.O) 1 2.2 Propylene glycol 5.0 Ethanol 3.0 Carboxyvinyl polymer (1% aqueous solution) 18.0 Potassium hydroxide 0.1 Preservative 0.3 Perfume Fine purified water Remainder 100.0

[0032]

[0033]

As described above in detail, the invention according to claim 1 relates to a cosmetic composition characterized by containing an Apiaceae sperm (Oenanthe storonifra, DC) and / or an extract thereof. Therefore, it gives moisture to the skin by the moisturizing effect, and it can be a fresh skin, and it exhibits a whitening effect by anti-tyrosinase activity, and can be effectively used as a cosmetic for whitening, It has an excellent effect that it can be used for suppressing or treating pigmentation diseases such as spots, freckles, and black and white.

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Claims (1)

[Claims] [Claim 1] A cosmetic composition characterized by containing an Apiaceae seri (Oenanthe storonifra, DC) and / or an extract thereof.