JP2000125900A5 - - Google Patents

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Publication number
JP2000125900A5
JP2000125900A5 JP1998301316A JP30131698A JP2000125900A5 JP 2000125900 A5 JP2000125900 A5 JP 2000125900A5 JP 1998301316 A JP1998301316 A JP 1998301316A JP 30131698 A JP30131698 A JP 30131698A JP 2000125900 A5 JP2000125900 A5 JP 2000125900A5
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JP
Japan
Prior art keywords
nucleic acid
target nucleic
acid molecule
amplification
molecules
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JP1998301316A
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English (en)
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JP2000125900A (ja
JP4262336B2 (ja
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Priority to JP30131698A priority Critical patent/JP4262336B2/ja
Priority claimed from JP30131698A external-priority patent/JP4262336B2/ja
Priority to DE1999150823 priority patent/DE19950823A1/de
Publication of JP2000125900A publication Critical patent/JP2000125900A/ja
Publication of JP2000125900A5 publication Critical patent/JP2000125900A5/ja
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Publication of JP4262336B2 publication Critical patent/JP4262336B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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【特許請求の範囲】
【請求項１】 試料中に存在する標的核酸分子を定量的に分析する方法において、
標的核酸分子の離れた２つの塩基配列領域に対してそれぞれ相補的である塩基配列を有する第１および第２のプライマ分子と標的核酸分子を含む生物学的試料とを含んだ混合液を核酸増幅が達成されるようにハイブリダイゼーション反応が可能な状態に維持する増幅工程、ここで、いずれか一方のプライマは追跡可能な標識分子で標識されており、少なくとも標識されたプライマの混合数は既知である、
少なくとも１回の増幅工程を実行した上記混合液の少なくとも一部を用いて、標識分子に関する測定データを得る測定工程、
上記想定データに基づいて標的核酸分子の分子数あるいは分子サイズを決定する決定工程、
とを有することを特徴とする標的核酸の定量分析方法。
JP30131698A 1998-10-22 1998-10-22 標的核酸の定量分析方法 Expired - Fee Related JP4262336B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP30131698A JP4262336B2 (ja) 1998-10-22 1998-10-22 標的核酸の定量分析方法
DE1999150823 DE19950823A1 (de) 1998-10-22 1999-10-21 Quantitatives Verfahren zum Analysieren einer Targetnukleinsäure

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP30131698A JP4262336B2 (ja) 1998-10-22 1998-10-22 標的核酸の定量分析方法

Publications (3)

Publication Number Publication Date
JP2000125900A JP2000125900A (ja) 2000-05-09
JP2000125900A5 true JP2000125900A5 (ja) 2005-12-08
JP4262336B2 JP4262336B2 (ja) 2009-05-13

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Family Applications (1)

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JP30131698A Expired - Fee Related JP4262336B2 (ja) 1998-10-22 1998-10-22 標的核酸の定量分析方法

Country Status (2)

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JP (1) JP4262336B2 (ja)
DE (1) DE19950823A1 (ja)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3910000B2 (ja) * 2000-03-27 2007-04-25 オリンパス株式会社 １塩基置換検出方法
DE60126711T2 (de) * 2000-03-27 2007-10-25 Olympus Co. Verfahren zum messen von polymorphismen
JP2001269198A (ja) * 2000-03-27 2001-10-02 Olympus Optical Co Ltd 多型遺伝子の型を決定する方法
JP4749622B2 (ja) * 2001-08-10 2011-08-17 オリンパス株式会社 Ｄｎａエンドヌクレアーゼ活性の検出法
JP2003177131A (ja) * 2001-12-11 2003-06-27 Olympus Optical Co Ltd 生物学的な結合親和性を検出する方法
JP2010178716A (ja) * 2009-02-09 2010-08-19 Olympus Corp 標的核酸分子の定量方法及び標的核酸分子定量キット
JP6921638B2 (ja) 2017-06-16 2021-08-18 株式会社東芝 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム

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