JP2000000087A - New culture medium for microorganism - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a new culture medium effective for promoting the growth of mushroom and useful microorganisms for e.g. garbage treatment by compounding a liquid extract from plant(s) belonging to Myrtaceae, Ginkgoaceae, leguminous family or Gramineae, and a liquid extract from edible fruits (dried product(s)). SOLUTION: This new culture medium is obtained by compounding a liquid extract from plant(s) belonging to Myrtaceae, Ginkgoaceae, leguminous family or Gramineae, and a liquid extract from edible fruits (dried product(s)). This culture medium is useful for culturing effective microorganisms usable for promoting the growth of mushroom's mycelia and useful microorganisms such as soil fungi used in e.g. garbage treatment. Furthermore, the mycelia of Agaricus blazei Murill can be cultured in this medium diluted with water or an aqueous medium to afford the mycelial extract of Agaricus blazei Murill having anticancer activity or carcinostatic activity as well as such activities as disease prevention, senility suppression and immunopotentiation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a microorganism culture medium. More specifically, the present invention relates to a microorganism culture medium that optimizes the growth environment of microorganisms such as soil fungi and fungi such as useful mushrooms.

[0002]

2. Description of the Related Art At present, in the field of waste treatment, methods for treating waste based on the action of various useful microorganisms have been proposed. One of them is a treatment of composting or decomposing by the action of microorganisms in garbage.

[0003] Composting of garbage (composting) is to put bark, sawdust, leaf fall, cultivated soil, etc. in a plastic container generally called a composter to propagate soil bacteria, and put garbage into this. On top of that, organic materials are added to help composting, and garbage is fermented to compost.

[0004] However, in such a composting method, there is a problem that the composting does not proceed well under the conditions of the weather or the like and that it takes time to compost. In addition, a method called EM method for fermenting garbage using anaerobic or microaerobic bacteria using an effective microorganism group (EffectiveMicroglobe) has also been proposed. In this method, care is also taken on the propagation conditions of microorganisms. Must also
It took a long time to compost.

As another method of treating garbage with microorganisms, there is a treatment method using a garbage disposal apparatus or a garbage ultra-reduction apparatus. This method is to reduce the volume and weight of garbage by decomposing the garbage into carbon dioxide, water, etc. by the action of aerobic microorganisms while stirring the garbage mainly in an atmosphere where there is a lot of oxygen. Microorganisms that regulate and decompose garbage water and materials such as sawdust to propagate these microorganisms. It is said that the garbage processed by this processor can be used as compost.

However, even in this method, the breeding environment of the microorganisms is not constant, and it is necessary to pay attention to the breeding conditions of the microorganisms, and there is a need for a base material with an enhanced action of decomposing the garbage of the microorganisms.

[0007] As an example of effective use of microorganisms, Japanese Patent Publication No. 42355/1992 discloses that rhizobia and Azotobacter or photosynthetic bacteria and sulfur bacteria are added to a solution of plant ash with sucrose or maltose and inoculated into a sterilized medium. Cultivate at about ℃ for an appropriate time, and separately prepare and mix culture solutions of nitrifying bacteria, yeast, thermophiles, Bacillus subtilis, and Pseudomonas bacteria to promote the maturation of compost, improve the soil, improve the fertilizer effect, and maintain It discloses that it has the ability to detoxify pesticides and suppress diseased microorganisms.

However, there has been a demand to further enhance the ability of the microorganisms contained therein.

On the other hand, in addition to the microorganisms used for composting and / or decomposing garbage, useful fungi include mushrooms. Mushrooms are used for food, and many mushrooms have been known to have many medicinal effects since ancient times, and have been used as health foods. In recent years, Agaricus mushrooms, Mesimakobu mushrooms, Kikobu mushrooms, Kaigara mushrooms, Matsutake mushrooms, Shiitake mushrooms, Kawara mushrooms, Hiratake mushrooms, Tsugarusarunokoshikatake mushrooms, Nameko mushrooms, Enoki mushrooms, Mannen mushrooms (Lingzhi)
It has been found that various mushrooms such as and the like have an anticancer effect and an anticancer effect in addition to the effects of preventing diseases, preventing aging, and improving immunity. Particularly recently, mushrooms called agaricus mushrooms have attracted attention.

Agaricus mushroom is a basidiomycete mushroom whose name is Agaricus blazeiMurill, and its origin is in the Piedate Mountains on the outskirts of Sao Paulo in Brazil. It has been found that this mushroom is rich in high-molecular polysaccharides such as β-glucan, phosphoric acid, and unsaturated lipids, and it is not clear how these components act. It has been found that, in addition to the anticancer effect, it has an outstanding effect in preventing disease, preventing aging, improving immunity, and the like.

Although it was very difficult to culture this mushroom, as a result of the study by the present inventors, the following steps were performed: (a) using a water-based culture solution, Agaricus blazei. Culture solution 1 on a medium capable of culturing myrils of muril
Culturing the mycelium of Agaricus brazei muril at 30 to 150 g per liter at 0 to 25 ° C. for at least one week, (b) bringing the liquid temperature to at least 30 ° C. over 1 to 4 days at a pressure of 1 to 10 atm. Raising and separating the mycelium of Agaricus blazei muril from the medium, (c)
While the aeration is continued under the same conditions as in step (b), the temperature of the solution is raised to 50 ° C. or more at a stretch and kept for 1 to 4 hours, and then cooled to room temperature and kept at this temperature for 1 to 15 hours. Performing the first filtration, (d) 15 to 30 ° C. for 1 to 4 hours
And then incubated at room temperature for 1 to 5 days while continuing aeration under the same conditions as in step (b) to allow the mycelium of Agaricus blazei muril to grow. After breeding, aeration is stopped and maintained for 12 to 36 hours, and a second filtration is performed while aeration is performed under the same conditions as in step (b). (E) Same as step (b) After incubating at room temperature for 2 to 7 hours while continuing aeration under the conditions of the above, the liquid temperature of the culture system is raised to at least 60 ° C. and kept at this temperature for 30 minutes to 4 hours to kill the germs that have proliferated,
Allowing to cool to room temperature and incubating at this temperature for 1 to 6 hours and performing a final filtration; (f) boiling the culture system for at least one day, and then allowing to cool to room temperature and cooling at this temperature for 1 hour; ~ 6
(G) Incubate at room temperature while performing aeration under the above conditions, and during this incubation, Agaricus blazei
Agaricus brazei muril hyphae, comprising the steps of adjusting the concentration of muril mycelium, (h) stopping aeration and incubating, and (i) keeping the aeration pressure as low as possible and incubating at room temperature. We found a method for producing body extract and filed a patent application.

In this production method, there is a need to further promote the cultivation of the mycelium of Agaricus blazei muril.

[0013]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a novel microorganism culture medium for culturing useful microorganisms used for treating garbage and the like and effective microorganisms which can be used to promote the growth of mushrooms. It is to provide.

[0014]

The present inventors have conducted intensive studies to solve the above-mentioned problems, and as a result, a mixed solution comprising an extract of a specific plant and an extract of a specific fruit solves the above-mentioned problems. This led to the present invention.

[0015] Accordingly, the present invention provides a method comprising the steps of: A) an extract of one or more plants selected from the group consisting of Myrtaceae, Ginkgo, Legume, and Poaceae; and B) a group consisting of edible fruits. The present invention relates to a novel microorganism culture medium comprising an extract of one or more selected fruits or a dried product thereof.

The present invention also relates to a microorganism growth promoting solution comprising the above-mentioned microorganism culture medium and water or an aqueous medium.
The growth promoting solution of the microorganism preferably comprises water activated by introducing a ceramic body whose aqueous medium has a catalytic action on the microorganism or by passing water through the ceramic body.

The present invention further relates to an active culture solution of the mycelium of mushrooms comprising the microorganism culture medium and water or an aqueous medium described above and, if necessary, yeast and saccharides. The culture active solution of the fungal mycelium of the mushroom is prepared by adding a ceramic body whose aqueous medium has a catalytic action to the mycelium of the mushroom,
Alternatively, it preferably comprises water activated by passing water through the ceramic body.

The present invention further relates to a method for treating a food waste material, which comprises immersing a food waste material comprising plant-derived cellulose in a solution for promoting the growth of the microorganism. The present invention further relates to a material for treating garbage obtained by the above method.

The present invention also relates to a method for producing a mushroom culture medium, comprising immersing a raw material of a mushroom culture medium selected from the group consisting of plant-derived cellulose in a culture active solution of the mycelium of the mushroom, and allowing to stand. .

The present invention further relates to a mushroom culture medium treated by the above method. The present invention also relates to a method for cultivating mushrooms, which comprises inoculating a fungal or edible mushroom mycelium into the mushroom culture medium and allowing the fungi to stand.

The present invention further relates to (a) using a water-based culture solution on a medium capable of culturing the mycelium of Agaricus blazei muril on a medium capable of culturing 30 to 1 per liter of culture solution.
50 g of Agaricus brazei muril mycelium
Culturing at 25 ° C. for at least one week, (b) 1-10
Liquid temperature at least 30 ° C over 1 to 4 days at atmospheric pressure
And separating the mycelium of Agaricus blazei muril from the above medium, (c) raising the liquid temperature to 50 ° C. or more at a stretch while continuing aeration under the same conditions as in step (b) for 1 to 4 hours. After the temperature is kept, it is allowed to cool to room temperature, kept at this temperature for 1 to 15 hours, and then performs the first filtration, (d) left at a temperature of 15 to 30 ° C. for 1 to 4 hours to form a culture system. After breeding the existing germs, aerating is continued under the same conditions as in step (b) and incubated at room temperature for 1 to 5 days to propagate the mycelium of Agaricus blazei muril, and then aeration is stopped. And performing a second filtration while aerating under the same conditions as in step (b). (E) Room temperature while continuing aeration under the same conditions as in step (b). After 2 to 7 hours incubation, raise the temperature of the culture to at least 60 ° C. And held at temperature for 30 minutes to 4 hours to kill bred the bacteria, allowed to cool to room temperature and 1-6 hours incubation at this temperature, and performing a final filtration,
(f) allowing the culture to boil for at least one day, then allowing it to cool to room temperature and incubating at this temperature for 1 to 6 hours;
(g) Incubating at room temperature while performing aeration under the above conditions, adjusting the concentration of mycelium of Agaricus blazei muril during this incubation, and (h) stopping aeration and incubating ,
And (i) a method for producing a mycelium extract of Agaricus blazei muril comprising lowering the aeration pressure as much as possible and incubating at room temperature, wherein in step (a) a culture solution of the mycelium is contained as a culture solution. The present invention relates to a method for producing a mycelium extract of Agaricus blazei muril, which comprises using a culture solution.

[0022]

The embodiments of the present invention will be described below. (Microbial culture medium) The biologically active solution according to the present invention comprises: A) an extract of one or more plants selected from the group consisting of Myrtaceae, Ginkgo, Legume, and Poaceae;
The plant belonging to A is composed of an extract of one or more fruits selected from the group consisting of edible fruits or a dried product thereof, and the plants belonging to A include pomegranate, clove, bunjiro,
Examples include plants of the family Myrtaceae such as Berberi tree, Myrtle, Eucalyptus and the like, ginkgo, sasa, bamboos, pampas grass, legumes, and the like, and one or more of these can be used in combination. These plants are whole plants,
A stem portion, a leaf portion and the like are extracted and used by a general extraction method.

The fruits belonging to B in the present invention include apricot, plum, peach, strawberry, grape, tomato,
Examples include bananas, apples, and pears, and these can be used alone or in combination of two or more. The fruit used in the present invention may be a dried product. These fruits are pulverized as necessary and extracted by a usual method.

When the extract of the component A and the extract of the component B are mixed as described above, a microorganism culture medium according to the present invention is produced. The mixing ratio of the component A and the component B is determined according to the present invention. There is no particular limitation as long as the purpose is achieved. Generally, the mixing ratio of the A component and the B component is 1:99 to 99: 1, preferably 30:70 to 70: 3 by volume ratio.
0, more preferably 40:60 to 60:40.

It has been found that the thus-produced culture medium of the microorganism of the present invention can be used for various purposes.

(Solution for Promoting the Growth of Microorganisms) The microorganism culture medium according to the present invention is prepared by adding water or an aqueous medium to
Additives that are diluted 0000-fold, preferably 1000-15000-fold, and are effective for microorganisms that grow as needed, for example, sugars such as sucrose, maltose, and fructose, yeasts such as brewer's yeast, sake yeast, baker's yeast, and wheat yeast. And the like can be used as a growth promoting solution of the microorganism according to the present invention.

At this time, the activation of the microorganisms is enhanced by introducing a ceramic body having a catalytic action on the microorganisms as an aqueous medium or by using water activated by passing water through the ceramic body. Is preferred.

(Application Example 1 of Microorganism Growth Acceleration Solution: Material for Garbage Disposal) The microorganism growth accelerating solution according to the present invention can be used for treating a material for treating garbage.

[Composting of garbage and reduction of garbage]
Bark, sawdust, defoliation, garbage disposal materials such as culture soil, immersed in the growth promoting solution of the microorganism according to the present invention, at room temperature for 24-150 hours, preferably 40-150 hours,
Leave under aeration. The material for garbage disposal is treated in this way, the treated material is put into the soil, soil bacteria are propagated, and the garbage is put into this for composting. As a result, the propagation of microorganisms is promoted, and it is relatively less affected by surrounding conditions such as weather. Similarly, when the material thus treated is used as a material for a garbage disposal device, compared to a conventional untreated material, the growth of microorganisms is promoted, and the material is relatively less affected by surrounding conditions. .

(Culture of soil fungi) JP-B-4-42355
As described in the official gazette, it is said that soil bacteria have the ability to promote the maturation of compost, improve the soil, increase the fertilizer effect, detoxify pesticide residues, and suppress diseased microorganisms. Therefore, rhizobia and Azotobacter or photosynthetic bacteria and sulfur bacteria were added to a solution of plant ash with sucrose or maltose and a growth promoting solution of the microorganism according to the present invention, inoculated into a sterilized medium, and cultured at about 25 ° C. for an appropriate time. Separately, a mixed culture of microorganisms prepared and mixed with a culture solution of nitrifying bacteria, yeast, thermophilic bacteria, Bacillus subtilis, and Pseudomonas in a growth promoting solution of the microorganism according to the present invention promotes maturation of compost, improves soil, and improves fertilizer effect. It has been found that the ability to enhance the activity, detoxify residual agricultural chemicals, and suppress diseased microorganisms has been further enhanced, and the ability to remove heavy metals such as Cd and Hg has been found.

As microorganisms intervening in this culture solution, for example, Rhizoium Japonicum, Rhizoiummelioti, Rhizoium
trifolii, Rhizoium leguminosarum, Rhizoium, Rhiz
Azotobacilli, such as oiumplaseobi and Rhizoium lupini
tor indicum, Azotobactor vinlandi, Azotobactor
Azotobacters such as chroococcum, Rhodopseudomonas
capsulatus, Clostridium Butyricum, Closteidium
Photosynthetic bacteria such as pasteurianum, Clostedium aceticum, Th
iobacillus thioparus, Thiobacillus thiooxidans, Th
Nitrosomonas, sulfur bacteria such as iobacillus denitrificans
europaea, Nitrosomonas oligocarbogenes, Nitrobao
Nitrifying bacteria such as ter winogradskyi, Nitrobaoter agilis, Ce
fibrinolytic bacteria such as llulomonas Flavigena, Streptomyces
actinomycetes such as griseus, Aspergillus Oryzae, Penicill
Filamentous fungi such as ium patulum and Mucor mucedo, Sacharomyc
yeasts such as es ellipsoideus, Bacillus subtilis such as Bacillus subtilis, Pseudomonas viscosissima, Pseudomonas albofl
ava, Pseudomonas fluorescens var, Pseudomonas ru
Pseudomonas such as hlamdii, other Bacilluspolymy
xa, Bacillus circulans, Bacillus cereus, Bacillus
posteurii, Bacillus freudenre, Bacillus megater
aerobic microorganisms belonging to the genus Bacillus such as ium,
It is believed that the synergistic effect of these microorganisms has such enhanced properties.

(Culture Activating Solution of Mycelium of Mushrooms) The microorganism culture medium according to the present invention can be used as a growth promoting solution for microorganisms as described above, or in water or an aqueous medium.
It can be diluted 0 to 20000 times, preferably 1000 to 15000 times, and can be used as a culture active solution of mycelia of mushrooms by adding yeast and saccharides as necessary.

As an aqueous medium that can be used at this time, a ceramic body having a catalytic action on the mycelium of mushrooms to be cultivated in the same manner as the above-mentioned growth promoting solution is added, or water is passed through the ceramic body It is preferable to use water activated by the activation, since the activation of microorganisms can be enhanced.

A mushroom culture medium material selected from the group consisting of plant-derived cellulose is immersed in such a mushroom mycelium culture active solution for 24 hours to 150 hours, preferably 4 hours.
When left for 0 to 100 hours, it is possible to produce a culture medium for growing mushrooms in which the growth rate of mycelium of mushrooms is increased.

For example, a medium such as sawdust is placed in a container and filled with a culture active solution of the mycelium of the mushroom according to the present invention until the volume becomes about 60% by volume. Can be produced. The mushrooms that can be cultivated in such a medium are not particularly limited as long as they are mushrooms that can usually be cultivated with sawdust or the like, and include, for example, oyster mushrooms, maitake, shimejitake, tamogitake, and enoki mushroom, and the like. Use increases productivity by 10-30%, generally about 15-25%.

(Cultivation of mycelium extract of Agaricus blazei muril) The culture active solution of mycelium of mushrooms of the present invention can also be used for cultivation of a mycelium extract of Agaricus brazei muril. Agaricus brazeimuril was said to be difficult to grow, but according to the present inventors, (a) a culture medium capable of culturing the mycelium of Agaricus blazeimuril using a water-based culture solution Culture solution 1 on
Incubate 30-150 g per liter of Agaricus brazei muril mycelium at 0-25 ° C. for at least one week, and (b) raise the liquid temperature to at least 30 ° C. over 1 to 4 days at a pressure of 1 to 10 atm. Agaricus brazei muril was separated from the culture medium, and (c) the solution temperature was increased to 50 ° C. or more at a stretch while maintaining aeration under the same conditions as in step (b), and then maintained for 1 to 4 hours. The mixture was allowed to cool to room temperature, kept at this temperature for 1 to 15 hours, then subjected to the first filtration, and (d) left at a temperature of 15 to 30 ° C for 1 to 4 hours to remove various bacteria existing in the culture system. After breeding, the agaricus brazei muril mycelium was propagated by incubating at room temperature for 1 to 5 days while continuing aeration under the same conditions as in step (b). Hold for 36 hours and under the same conditions as in step (b) A second filtration was carried out with care, and after (e) incubation at room temperature for 2-7 hours while continuing aeration under the same conditions as in step (b), the liquid temperature of the culture system was reduced to at least 60 ° C. Raise and hold at this temperature for 30 minutes to 4 hours to kill the propagated germs, allow to cool to room temperature, incubate at this temperature for 1 to 6 hours, perform final filtration, and (f) After boiling for one day, allow to cool to room temperature and incubate at this temperature for 1 to 6 hours. (G) Incubate at room temperature with aeration under the above conditions.・ Adjust the concentration of mycelium of Brazei Murrill,
(h) stop aeration and incubate; and (i) reduce the aeration pressure as much as possible and incubate at room temperature.
It has been found that it is possible to produce a myril extract of muril.

In this method, first, the mycelium of Agaricus blazei muril is grown to grow the mycelium of Agaricus blazei muril. At this time, the culture medium of the mushroom mycelium of the present invention is used as a culture medium. It has been found that the use of an active solution increases the growth rate.

[0038]

As described above, A) 1 selected from the group consisting of Myrtaceae, Ginkgo, Legume and Gramineae
A novel microbial culture comprising an extract of one or more plants and an extract of one or more fruits selected from the group consisting of edible fruits or a dried product thereof, comprising: It promotes the growth of useful microorganisms and also promotes the growth of mycelium of mushrooms.

When the above-mentioned microorganism culture medium is diluted with water or an aqueous medium, a solution for promoting the growth of microorganisms is obtained, and the obtained solution can be suitably used for treating materials for treating garbage. The material treated with this solution has a higher microbial propagation environment than conventional untreated materials.

Further, the above-mentioned microorganism culture medium is diluted with water or an aqueous medium, and if necessary, yeast, saccharides and the like can be added to use as a solution for promoting the growth of mycelia of mushrooms. Cultivation can be performed efficiently, and can also be used as a culture medium when producing a mycelium extract of Agaricus blazei muril,
It is possible to increase the growth rate of mycelium.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C12N 1/38 C12N 1/38 F term (Reference) 2B011 AA07 BA12 BA13 GA04 GA08 GA09 GA12 4B018 LE05 MG02 MJ01 MJ02 MP04 MS01 MS04 MS06 MS11 4B065 AA01X AA57X AC20 BB01 BB14 BB26 BB29 BB40 BC01 BC03 BC05 BC41 BC50 CA55 CA60

Claims (11)

[Claims] 1. A) Myrtaceae, Ginkgoaceae, Legumes,
It comprises an extract of one or more plants selected from the group consisting of Poaceae and B) an extract of one or more fruits selected from the group consisting of edible fruits or an extract thereof. New microorganism culture medium. 2. A solution for promoting the growth of microorganisms, comprising the microorganism culture medium according to claim 1 and water or an aqueous medium. 3. The microorganism according to claim 2, wherein the aqueous medium comprises water activated by charging a ceramic body having a catalytic action on the microorganisms or by passing water through the ceramic body. Growth promoting solution. 4. A culture active solution of a mycelium of a mushroom comprising the microorganism culture medium according to claim 1 and water or an aqueous medium, and if necessary, yeast and saccharides. 5. The method according to claim 4, wherein the aqueous medium comprises water activated by introducing a ceramic body having a catalytic action on mycelium of mushrooms or by passing water through the ceramic body. An active culture solution of mycelium of mushrooms. 6. A method for treating garbage, comprising immersing the garbage disposal material comprising plant-derived cellulose in the solution for promoting the growth of microorganisms according to claim 2 or 3. 7. A garbage disposal material obtained by the method according to claim 6. 8. A mushroom culture medium characterized in that a mushroom culture medium material selected from the group consisting of plant-derived cellulose is immersed in the culture active solution of the mushroom mycelium according to claim 4 or 5, and the mixture is allowed to stand. Manufacturing method. 9. A mushroom culture medium obtained by the method according to claim 8. 10. A method for cultivating mushrooms, comprising inoculating the mushroom culture medium according to claim 9 with a mycelium of useful mushrooms or edible mushrooms and allowing to stand. 11. A mycelium of Agaricus blazei muril on a medium capable of cultivating a mycelium of Agaricus blazei mullil using a water-based culture medium in an amount of 30 to 150 g per liter of culture liquid. Culturing the body at 0-25 ° C. for at least one week, (b) raising the temperature of the liquid to at least 30 ° C. over 1 to 4 days at a pressure of 1 to 10 atm, and transferring the mycelium of Agaricus blazei muril to the above medium. (C) While the aeration is continued under the same conditions as in step (b), the liquid temperature is raised to 50 ° C. or more at a stretch and kept for 1 to 4 hours, and then allowed to cool to room temperature. 1 to 15
Holding time and then performing the first filtration, (d) 1 to
After standing at a temperature of 15 to 30 ° C. for 4 hours to propagate various bacteria present in the culture system, the agaricus was incubated at room temperature for 1 to 5 days while continuing aeration under the same conditions as in step (b).
Stopping the aeration after propagating the mycelium of Brazei muril and holding for 12 to 36 hours, and performing a second filtration with aeration under the same conditions as in step (b), (e) While maintaining aeration under the same conditions as in step (b),
After incubating for 7 hours, the temperature of the culture system is raised to at least 60 ° C. and kept at this temperature for 30 minutes to 4 hours to kill the germs that have grown, and then left to cool to room temperature and left at this temperature for 1 to 6 hours. Incubating and performing final filtration, (f) boiling the culture system for at least one day, allowing it to cool to room temperature, and incubating at this temperature for 1 to 6 hours, (g) the above conditions Incubating at room temperature while performing aeration under, adjusting the concentration of mycelium of Agaricus blazei muril during this incubation,
In the method for producing a mycelium extract of Agaricus blazei muril, which comprises the steps of (h) stopping the aeration and incubating, and (i) incubating at room temperature with the aeration pressure reduced as much as possible, step (a) A method for producing a mycelium extract of Agaricus blazei muril, comprising using a culture solution containing the mycelium culture active solution according to claim 4 or 5 as the culture solution.