JP2000041623A - Production of mycelium essence of pleurotus eryngii - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for producing a mycelium essence of Pleurotus eryngii which the mycelium essence of the Pleurotus eryngii containing a pharmacodynamically effective ingredient or a nutritionally valuable ingredient can be obtained from a solid culture medium containing bagasse containing the mycelium of the Pleurotus eryngii as a base material in a short time without regulating the pH and a solid residue consisting essentially of the bagasse fibers which are by-products can effectively be utilized for a fertilizer, a feed or a food. SOLUTION: A fungus Pleurotus eryngii is inoculated onto a solid culture medium containing bagasse as a base material and cultured under consitions of 14-25 deg.C temperature and >70% humidity to proliferate the mycelium. The resultant solid culture medium containing the mycelium is untied and water and one or more kinds of enzymes selected from cellulase, protease, glucosidase hemicellulase chitinase, amylase and pectinase are added to the untied solid culture medium while keeping the solid culture medium at 30-50 deg.C temperature. The resultant solid culture medium is then pulverized and milled in the presence of the enzymes to extract an essence, which is subsequently heated at a temperature up to 95 deg.C to thereby inactivate the enzymes. Sterilization is carried out at the same time.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for producing an eryngii mycelium extract, and more particularly, to various effective methods from a solid medium containing a mycelium obtained by growing eryngii bacteria on a solid medium based on Bacass. The present invention relates to a method for producing an eryngium mycelium extract containing a component.

[0002]

2. Description of the Related Art Pleurotuseryngii, a kind of mushroom belonging to the genus Pleurotus
Is a phagocytic fungus distributed in regions such as Central-Southern Europe and Central Asia. With the spread and diversification of food culture, this eringi has come to the table in Japan, but it is generally difficult to grow it.
Various cultivation methods have been researched and developed. At present, cultivation methods cultivated in Kagawa Prefecture, Nagano Prefecture, and the like have come to be marketed on a ragged market.

[0003] The following are examples of cultivation methods for eryngii. For example, Japanese Patent Application Laid-Open No. 9-140285 discloses that a first pre-lotus eryngii containing less than 20% by weight of trehalose in a dry state is mixed with a first pre-lotus eryngii in a dry state.
A method for producing a new strain of Prelotus eryngii by crossing a second Prelotus eryngii containing trehalose by weight or more, according to this method, is disclosed. It states that it can be manufactured in a short time.

Japanese Patent Application Laid-Open No. 7-184473 discloses that
After filling a container such as a cultivation bottle with a medium material mixed with sawdust and a nutrient source and adjusting the water content, after heat-sterilizing the medium material, a seed of Pleurotus mushroom eryngii is inoculated on a culture medium, and the room temperature is 20 to An artificial cultivation method of eryngii, which is cultured at 23 ° C and a humidity of 65 to 70% and then grown at a room temperature of 15 to 19 ° C and a humidity of 80 to 90%, is disclosed. It states that it can be produced easily and reliably.

By the way, the present inventors have analyzed and studied the active ingredient of this eryngii, and found that this eryngii (bearing body) contains various amino acids such as arginine and lysine in a larger amount than Shiitake mushrooms. Contains a high concentration of nutritionally valuable active ingredients such as proteins and minerals, and efficiently extracts the medicinal or nutritionally valuable active ingredients from this eryngii, If it can be used as an extract,
Its significance is significant, and it is expected that the field of use will be further expanded.

[0006] Therefore, the present inventors have conducted extensive studies on a method for extracting a medicinal ingredient or a nutritionally valuable active ingredient from eryngii in an efficient manner and using the same as an eryngii extract. Eryngii (solid fruit body)
In addition to finding that the medicinal and nutrient components are contained in a greater amount than the solid medium containing the Eryngium mycelium, which is obtained by culturing this myelin mycelium in a specific manner, an extremely large amount of medicinal and nutrient components is obtained. And that if the solid medium containing the eryngium mycelium is treated by a specific method, an eryngium mycelium extract containing a large amount of excellent medicinal and nutrient components can be obtained efficiently. And found that the present invention was completed.

[0007] Each of the above publications relates to a cultivation method of eryngii, and it is not clear how to obtain an eryngium mycelium extract containing a remarkably large amount of medicinal and nutrient components. There is no description or suggestion.

The medicinal properties of basidiomycetes such as shiitake mushrooms, which are different mushrooms from eryngii, have been widely studied, and a medicinal or nutritionally valuable active ingredient is extracted from the mycelium of shiitake mushrooms. Various methods have been proposed.

For example, Japanese Patent Publication No. 51-19013 discloses that a solid medium comprising sawdust and rice bran or the like is inoculated with a Shiitake mushroom inoculum, and the mycelium is grown by a conventional method. The medium was crushed and water was added to adjust the pH to 5.0.
The mixture was sealed in a container and heated to 30 to 55 ° C. to promote the metabolism of mycelial enzymes and metabolites. After the enzyme reaction was sufficiently performed, the suspension was filtered. A method of extracting a medicinal component from a solid culture mycelium of shiitake mushrooms is disclosed.

Japanese Patent Publication No. 53-23392 discloses a solid medium comprising a peanut epidermis or bacass medium and, if necessary, rice bran, inoculated with Shiitake fungi, After growing the mycelium, the medium containing the mycelium was pulverized, water adjusted to pH was added thereto, and the medium was sealed in a container.
A method for producing a health drink, comprising heating the suspension to about 55 ° C. to promote the metabolism of the mycelium and sufficiently performing the enzymatic reaction, and then filtering the resulting suspension. I have.

Japanese Patent Publication No. 23826/1985 discloses that a solid medium containing mycelium obtained by inoculating Shiitake fungi on a solid medium based on Bacus and then growing the mycelium is disclosed in The bundle is unbundled so that the mesh passing amount is 30% by weight or less. One or more of water and an enzyme selected from cellulase or protease are added to the unbundled solid medium at 30 to 50 ° C. The solid medium is crushed and crushed in the presence of the enzyme so that at least 70% by weight of the Bacass fiber passes through the 12 mesh,
A method of producing a health drink, which comprises deactivating and sterilizing the enzyme by heating to a temperature of up to 200 ° C. and filtering the resulting suspension.

Further, Japanese Patent Application No. 59-5355 (Japanese Patent Publication No. 4-35149) and Japanese Patent Application No. 59-5356 (Japanese Patent Application No. 4-6171) disclose health drinks using Reishi bacillus. A manufacturing method is described.

[0013] However, these publications do not disclose or suggest any method for producing an eryngii mycelium extract.

[0014]

SUMMARY OF THE INVENTION The object of the present invention is to solve the problems associated with the prior art described above, and to provide a medicinal component or nutrient from a bacass-based solid medium containing myring mycelium. Eryngii mycelium extract containing a valuable component can be obtained in a short time without adjusting the pH, and a solid residue fertilizer, feed or food mainly composed of bacass fiber as a by-product It is an object of the present invention to provide a method for producing an eryngii mycelium extract that can be effectively used for Escherichia coli.

Another object of the present invention is to provide a method for producing a solid medium based on bacas containing eryngii mycelium, which contains a medicinal ingredient or a nutritionally valuable ingredient at a high concentration. .

[0016]

SUMMARY OF THE INVENTION That is, the method for producing an eryngium mycelium extract according to the present invention comprises the steps of:
Eryngii fungi are inoculated, cultured under conditions of a temperature of 14 ° C. to 25 ° C. and a humidity of more than 70%, and a solid medium containing mycelium obtained by growing mycelium is unbundled. Water and one or more of enzymes selected from cellulase, protease, glucosidase, hemicellulase, chitinase, amylase, pectinase are added to the solid medium while maintaining the solid medium at a temperature of 30 to 50 ° C., and The solid medium is ground and crushed in the presence of an enzyme to extract an extract, and then heated to a temperature of up to 95 ° C. to inactivate and sterilize the enzyme.

In a preferred embodiment of the present invention, the solid medium is desirably crushed and crushed in the presence of an enzyme as described above, so that at least 70% by weight or more of the Bacass fiber passes through 12 mesh.

The eryngii mycelium extract obtained by such a method has a urinary action and a fatigue recovery effect when ingested, and has a moisturizing effect when applied to the skin. Even when used, there is little risk of side effects and the safety is excellent.

[0019]

DETAILED DESCRIPTION OF THE INVENTION Hereinafter, the method for producing the eryngium mycelium extract according to the present invention will be specifically described.

In the present invention, first, water, preferably pure water, is appropriately mixed into a solid medium consisting of Bacas, and then Eryngii bacteria are inoculated. In addition, besides rice bran,
If necessary, minerals such as phosphorus and iron, peanut epidermis, brown rice and the like may be added. In addition, the sugarcane squeezed bacas contains sugars and proteins that are nutrient sources of mycelium, and can be used as a solid medium as it is. Rice bran is usually used in an amount of about 10 to 30 parts by weight with respect to 100 parts by weight of Bacchus.

As the Eryngii fungi, those known in the art can be used, and new bacteria described in JP-A-7-184473 and new strains described in JP-A-9-140285 can also be used. it can.

Next, the culture medium inoculated with the erythromycetes is placed in a culture room in which the temperature and humidity are controlled and the illuminance is also controlled, and the mycelium is grown. When the eryngii mycelium is propagated in this way, after inoculating the eryngii inoculum as described above, under conditions of a temperature of 14 to 25 ° C. and a humidity of more than 70%, preferably the above temperature and humidity of 75 to 99% Under the conditions described above, it is particularly preferable to maintain (cultivate) the mycelium for usually 2 to 6 months, preferably 3 to 4 months in a culture chamber adjusted to the above-mentioned conditions of temperature and humidity of 80 to 95% to grow mycelium. It is desirable to make it.

[0023] The medium components are decomposed during the cultivation by the action of Eryngii fungi, and finally become water and carbon dioxide, and the water content in the medium becomes about 80%. Therefore, the humidity is set to 7
If it is set to 0% or less, the evaporation of water to the outside of the culture medium increases, and as a result, the culture medium shrinks, and the intended object cannot be obtained.

In the present invention, the mycelium spreads in the solid medium, and immediately before and immediately after the occurrence of fruiting bodies, the fibrous material of the bacass base material is unbundled, and the passing through 12 mesh becomes 30% by weight or less. It is desirable to do so. It is preferable that the unwinding of the bacass base medium be performed immediately before and after the occurrence of the fruiting body as described above, but it may be performed at a time after the fruiting body has grown considerably.

The solid medium thus unbundled is mixed with water and one or more enzymes selected from the group consisting of cellulase, protease, glucosidase, hemicellulase, chitinase, amylase and pectinase. Add while keeping. As the enzyme to be added, cellulase is preferable.

The amount of the enzyme to be added is preferably 0.5 to 5 g, preferably 1 to 3 g, per kg of the solid medium. The water is preferably pure water containing no ions such as metal ions, and 1 kg of pure water, preferably 3 to 5 kg of pure water is added to 1 kg of the unbundled medium to form a mixture containing bacass.

Next, the eryngium mycelium extract is extracted from the Bacchus-containing mixture. To extract the eryngium mycelium extract, the medium-containing mixture is circulated using, for example, a gear pump with a transmission. Crushing and crushing the solid medium to reduce
It is desirable that not less than 12 wt% pass through 12 mesh. If the amount passed through the 12 mesh is less than 70% by weight, the extraction of the active ingredient in the solid medium becomes insufficient, the portion where the fibrin is not sufficiently softened increases, and the obtained solid residue is used as feed, food or In some cases, it cannot be used effectively as fertilizer.

The pulverization and crushing of the mixture containing Bacass may be carried out while maintaining the temperature of the mixture at 30 to 50 ° C., or may be carried out while gradually increasing the temperature from the above temperature. It is preferable to perform while. When the water temperature is 30 ° C. or higher, preferably 35 ° C. or higher,
When air at room temperature is blown into the mixture containing bacass, the air bubbles are rapidly heated and destroyed, impacting the bacass fibers, and extracting the active ingredient more efficiently.

Next, the thus-treated mixture containing bacass is further heated to a temperature of up to 95 ° C., preferably about 75 to 90 ° C., and maintained at this temperature for several tens of minutes. Inactivate the enzymes in the mixture,
When the mixture is sterilized, an eryngii mycelium extract is obtained. When the enzyme in the mixture is inactivated by heating and sterilized, deterioration of the eryngium mycelium extract can be prevented.

If necessary, the obtained eryngii mycelium extract may be treated with 50 to 120 mesh, preferably 60 to 1 mesh.
You may filter using the filter cloth of about 00 mesh. The eryngium mycelium extract thus obtained has a higher oligosaccharide content than the mycelium extracts of other mushrooms.
Such an extract can be used after being concentrated or freeze-dried and used as a powder. Further, it can be used after being diluted with, for example, purified water, alcohol, or the like. Dermatitis treatment, athlete's foot treatment, comedone treatment, immune activator, AIDS treatment, anticancer agent, etc., cosmetics (skin moisturizing cosmetics, etc.), health drinks, soft drinks, confectionery, noodles, etc. It can be used by adding to foods and drinks, and can also be used by adding to various seasonings such as miso, soy sauce, and chemical seasonings.

[0031]

According to the present invention, an Eryngium mycelium extract containing a high concentration of a medicinal ingredient or a nutritionally valuable ingredient can be obtained from a bacas-based solid medium containing Eryngium mycelium in a short time. Can be obtained at The solid residue mainly composed of bacass fiber, a by-product, can be effectively used as fertilizer, feed or food.

[0032]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples, but the present invention is not limited to these examples.

[0033]

Example 1 A solid medium consisting of 70 parts by weight of Bacas and 30 parts by weight of rice bran was appropriately mixed with pure water, then inoculated with eryngium spp., And placed in a culture chamber adjusted to a temperature of 14 to 25 ° C. and a humidity of 85%. And allowed the mycelium to grow for 4 months. (More specifically, as for the temperature conditions, the initial culture is initially performed at 22 to 23 ° C. for one month from the initial stage of culture, the bacteria are activated in the medium, and then the temperature is gradually lowered to 14 ° C. over one month. The mycelium is cultivated for 2 months while maintaining the temperature at 14 ° C. to suppress the decomposition of the useful components of the medium.) After the mycelium has spread on the solid medium in this manner, The fibrous material was unbundled so that the amount passed through 12 meshes was 24% by weight or less. To 1.0 kg of the unbundled medium, 3.5 liters of pure water and 2.0 g of purified cellulase were added while maintaining the solid medium at 40 ° C., to obtain a baccus-containing mixture.

Then, while circulating the medium-containing mixture with a gear pump equipped with a transmission, a crushing and crushing action is applied to the solid medium for about 200 minutes in the gear portion of the pump, so that about 80% by weight of the Bacas fiber passes through 12 mesh. I made it. The pulverization and crushing of the Bacass-containing mixture were performed while gradually increasing the temperature of the mixture.

Thereafter, the mixture containing Bacass was further heated to 90 ° C. and left for 30 minutes. The enzyme was inactivated by heating to 90 ° C. and sterilized.

The resulting culture medium-containing mixture was filtered using a 60-mesh filter cloth to obtain an Eryngii mycelium extract containing fine suspended matter. This eryngii mycelium extract contained a large amount of medicinal components and nutrients.

The lyophilized powder of the Eryngii mycelium extract was analyzed. 100 g of a powder obtained by freeze-drying the extract contained 28.6 g of total sugars, of which 9.3 g of oligosaccharides (trehalose and the like) was contained.

Table 1 also shows the analysis results of the component compositions.
When this extract was taken orally several times a day for several days, the above-mentioned effects, that is, diuretic effect and fatigue recovery effect were observed. When applied to the skin several times a day as a skin cosmetic, a moist feeling was obtained.

As the solid residue, a sufficiently finely pulverized product was obtained, which was dried and provided as feed for livestock such as cattle, horses and pigs.

[0040]

Comparative Example 1 A lyophilized powder of mycelium of Shiitake mushroom was obtained in the same manner as in Example 1 except that Shiitake mushroom was used in place of Eryngii inoculum, and the powder was analyzed.

Table 1 shows the results.

[0042]

[Table 1]

The carbohydrate composition of each example is as follows. Ingredient composition of 100 g of saccharide of Example 1: 15.6 g of oligosaccharide in 48.0 g of total saccharide.

Composition of 100 g of saccharide of Comparative Example 1: 1.3 g of oligosaccharide in 27.5 g of total saccharide.

The amino acid composition of the protein in each Example and Comparative Example was analyzed by an automatic amino acid analysis method. Table 2 shows the results. However, the amount of each amino acid is indicated by the number (g) in 100 g of freeze-dried powder of Eryngii mycelium extract.
In addition, tryptophan was measured by the high performance liquid chromatography method.

[0046]

[Table 2]

[0047]

Example 2 The same procedure as in Example 1 was carried out except that 1.5 g of purified cellulase and 0.5 g of purified protease were added instead of 2.0 g of purified cellulase as enzymes to be added. Eryngii mycelium extract was obtained.

The extract was freeze-dried in the same manner as in Example 1 to obtain a powder, which was subjected to the same test as described above. As a result, the same result as in Example 1 was obtained.

Claims (2)

[Claims] 1. A mycelium obtained by inoculating a bacillus eryngii on a solid medium based on Bacass, culturing it at a temperature of 14 to 25 ° C. and a humidity of more than 70%, and growing the mycelium. Is unbound, and one or more of water and an enzyme selected from the group consisting of cellulase, protease, glucosidase, hemicellulase, chitinase, amylase and pectinase are added to the unbound solid medium. While maintaining the temperature at 30-50 ° C, the solid medium is crushed and crushed in the presence of the enzyme to extract the extract, and then the enzyme is inactivated by heating to a temperature of up to 95 ° C and A method for producing an eryngium mycelium extract, which comprises sterilizing. 2. The cultivation of the Eryngii fungus is carried out at a temperature of 14-25.
The method according to claim 1, which is carried out at a temperature of 75 ° C and a humidity of 99%.