IT202000002779A1 - Natural system to combat the Xylella Fastidiosa bacterium - Google Patents

Natural system to combat the Xylella Fastidiosa bacterium Download PDF

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IT202000002779A1
IT202000002779A1 IT102020000002779A IT202000002779A IT202000002779A1 IT 202000002779 A1 IT202000002779 A1 IT 202000002779A1 IT 102020000002779 A IT102020000002779 A IT 102020000002779A IT 202000002779 A IT202000002779 A IT 202000002779A IT 202000002779 A1 IT202000002779 A1 IT 202000002779A1
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present
composition according
mixture
essential oils
solution
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IT102020000002779A
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Italian (it)
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Roberto Taschini
Giancarlo Landi
Aldo Fittante
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Giancarlo Landi
Roberto Taschini
Aldo Fittante
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Priority to IT102020000002779A priority Critical patent/IT202000002779A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/42Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint

Description

DESCRIZIONE (versione corretta) DESCRIPTION (corrected version)

La presente invenzione ? relativa ad una composizione da utilizzare per combattere il batterio della Xylella Fastidiosa ed un metodo per la lotta al batterio. The present invention? concerning a composition to be used to fight the Xylella Fastidiosa bacterium and a method for fighting the bacterium.

Il batterio della Xylella Fastidiosa, con le sue varie sottospecie batteriche, ? un batterio gram-negativo che non forma spore, che attacca i vasi xylematici, corrispondenti ai vasi sanguigni umani, di molte piante produttive tra le quali possono essere citate, a titolo di esempio non limitativo, olivi, viti, agrumi, alberi del caff?, oltre a varie piante ornamentali e di altro tipo. The Xylella Fastidiosa bacterium, with its various bacterial subspecies,? a gram-negative bacterium that does not form spores, which attacks the xylematic vessels, corresponding to human blood vessels, of many productive plants among which can be cited, by way of non-limiting example, olive trees, vines, citrus fruits, coffee trees? , as well as various ornamental and other plants.

L?effetto dell?attacco da parte del batterio della Xylella Fastidiosa, ? che le piante colpite, dopo un certo tempo, variabile in base al tipo di pianta, si seccano, dando luogo pertanto a gravi perdite dei relativi prodotti agricoli, con conseguenti seri problemi economici per le aziende agricole direttamente colpite e per le attivit? ad esse collegate. The effect of the attack by the Xylella Fastidiosa bacterium,? that the affected plants, after a certain time, which varies according to the type of plant, dry up, thus giving rise to serious losses of the relative agricultural products, with consequent serious economic problems for the farms directly affected and for the activities? connected to them.

Per contrastare il batterio Xylella Fastidiosa, sono note miscele di natura chimica, cio? di sintesi, come il fertilizzante CE denominato Dentamet, costituito dal 2% di Rame e dal 4% di Zinco, complessati con acido citrico, oggetto di un brevetto Israeliano. To counteract the Xylella Fastidiosa bacterium, mixtures of a chemical nature are known, that is? synthetic, such as the CE fertilizer called Dentamet, consisting of 2% Copper and 4% Zinc, complexed with citric acid, the subject of an Israeli patent.

Il brevetto US10499651 B2 descrive un metodo per ridurre i sintomi di Xylella Fastidiosa basato su nuovi batteriofagi virulenti comprendenti XFAMIJA e XFAMIJO. US10499651 B2 describes a method for reducing the symptoms of Xylella Fastidiosa based on new virulent bacteriophages including XFAMIJA and XFAMIJO.

Il documento US2005/257285 A1 descrive un metodo per trattare la malattia di Pierce?s sulle viti, basato su proteine anti-microbiche comprendenti un polipeptide, fuso con un altro polipeptide di lisi batterica, per arrivare a rompere la membrana del batterio Xylella Fastidiosa. Document US2005 / 257285 A1 describes a method for treating Pierce's disease on vines, based on anti-microbial proteins comprising a polypeptide, fused with another bacterial lysis polypeptide, to break the membrane of the bacterium Xylella Fastidiosa.

Il documento WO2008/094684 A1 descrive peptidi ibridi, atti a conferire resistenza a fitopatogeni in piante transgeniche di Vitis Vinifera che, esprimendo un peptide di detta invenzione mostrano attivit? anti-microbica contro Xylella Fastidiosa. Document WO2008 / 094684 A1 describes hybrid peptides, suitable for conferring resistance to phytopathogens in transgenic plants of Vitis Vinifera which, expressing a peptide of said invention, show activity? anti-microbial against Xylella Fastidiosa.

Inoltre, in US10499651 B 2, dal titolo "Method and treatment for control of plant disease" ? descritto l?uso di agenti batteriofagi, contro il batterio Xylella, inseriti all'interno del batterio Xanthomonas". Also, in US10499651 B 2, entitled "Method and treatment for control of plant disease"? described the use of bacteriophage agents, against the Xylella bacterium, inserted inside the Xanthomonas bacterium ".

Il documento WO2014/139897 A1 descrive l?insetticida chimico Dithiinatetracarboximide per opporsi all?infezione da Xylella Fastidiosa. Document WO2014 / 139897 A1 describes the chemical insecticide Dithiinatetracarboximide to oppose Xylella Fastidiosa infection.

Sono note inoltre applicazioni del prodotto chimico N-acetilcisteina per tentare di distruggere il biofilm creato dal batterio Xylella Fastidiosa, con scarsi risultati. Applications of the chemical N-acetylcysteine are also known to attempt to destroy the biofilm created by the bacterium Xylella Fastidiosa, with poor results.

? noto un lavoro scientifico del Prof. del Dipartimento di Scienze Biologiche - Del UDR INSTM di Lecce che descrive l?inoculazione di cristalli del prodotto chimico carbonato di calcio nelle radici della pianta colpita da Xylella Fastidiosa per tentare di distruggere la membrana di detta Xylella Fastidiosa. ? I note a scientific work by Prof. of the Department of Biological Sciences - Del UDR INSTM of Lecce which describes the inoculation of crystals of the chemical calcium carbonate in the roots of the plant affected by Xylella Fastidiosa to try to destroy the membrane of said Xylella Fastidiosa.

Un inconveniente relativo all?impiego delle miscele di natura chimica ? rappresentato dal possibile danno causato alle piante trattate, danno che, tra l?altro non appare accompagnato dalla certezza di un?efficace e definitiva azione di contrasto contro la Xylella Fastidiosa. A drawback related to the use of mixtures of a chemical nature? represented by the possible damage caused to the treated plants, damage which, among other things, does not appear to be accompanied by the certainty of an effective and definitive action against Xylella Fastidiosa.

Un altro inconveniente dei tentativi di soluzione del problema ? legato alle applicazioni legate all?ingegneria genetica ed ? relativo alla loro complessit? tecnica ed applicativa. Another drawback of the attempts to solve the problem? linked to applications related to genetic engineering and? relative to their complexity? technical and applicative.

Inoltre, la Legislazione vigente in molti Stati, in particolare in Europa, impone che, per contrastare il batterio della Xylella Fastidiosa vengano utilizzati prodotti di derivazione naturale. In addition, the legislation in force in many states, especially in Europe, requires that products of natural derivation are used to combat the Xylella Fastidiosa bacterium.

Tra gli scopi della presente invenzione vi ? quello di risolvere gli inconvenienti summenzionati con una composizione che comprende una miscela completamente naturale, essendo detta miscela costituita da una serie di oli essenziali, denominati come segue con i rispettivi termini scientifici ufficiali: Allium Sativum, Thymus Vulgaris, Santalum Album, Origanum Vulgare, Pelargonium Graveolens. Among the objects of the present invention there is? that of solving the aforementioned drawbacks with a composition that includes a completely natural blend, being said blend consisting of a series of essential oils, named as follows with their respective official scientific terms: Allium Sativum, Thymus Vulgaris, Santalum Album, Origanum Vulgare, Pelargonium Graveolens.

Preferibilmente, di detti oli essenziali vengono utilizzate le tinture madre, essendo dette tinture madre preparate diluendo le parti secche di detti oli essenziali con una soluzione idroalcolica, essendo detta soluzione idroalcolica composta da acqua ed alcol etilico a differente gradazione, e detta diluizione avendo luogo nella proporzione di una parte di detto olio essenziale per dieci parti di detta soluzione idroalcolica, essendo dette parti calcolate come rispettive percentuali in peso, come sar? meglio descritto nel seguito. Preferably, mother tinctures are used of said essential oils, since said mother tinctures are prepared by diluting the dry parts of said essential oils with a hydroalcoholic solution, said hydroalcoholic solution being composed of water and ethyl alcohol of different strength, and said dilution taking place in the proportion of a part of said essential oil to ten parts of said hydroalcoholic solution, said parts being calculated as respective percentages by weight, how will it be? better described below.

La detta soluzione idroalcolica di detti oli essenziali viene preferibilmente somministrata alle piante colpite dal batterio di Xylella Fastidiosa contenuta all?interno di un?apposita siringa, in modo che, per il tramite della siringa, venga iniettata la soluzione idroalcolica dei detti oli essenziali nei pressi della base del tronco delle piante colpite, in modo che detta soluzione idroalcolica entri nei vasi xylematici delle dette piante colpite per contrastare il batterio di Xylella Fastidiosa, con le sue varie sottospecie batteriche. The said hydroalcoholic solution of said essential oils is preferably administered to the plants affected by the Xylella Fastidiosa bacterium contained inside a special syringe, so that, through the syringe, the hydroalcoholic solution of said essential oils is injected near of the base of the trunk of the affected plants, so that said hydroalcoholic solution enters the xylematic vessels of the said affected plants to counteract the Xylella Fastidiosa bacterium, with its various bacterial subspecies.

Verranno di seguito descritti i summenzionati oli essenziali. The aforementioned essential oils will be described below.

L?Allium Sativum si esplicita in lingua italiana anche come olio essenziale di aglio, essendo detto olio essenziale presente nella miscela che costituisce la composizione oggetto dell?invenzione con una quantit? percentuale in peso compresa fra l?1% ed il 3% in peso di detta miscela, l?Allium Sativum contenendo come principio attivo l?allicina. L? Allium Sativum is also explicit in Italian as essential oil of garlic, being said essential oil present in the mixture that constitutes the composition object of the invention with a quantity? percentage by weight between 1% and 3% by weight of said mixture, Allium Sativum containing allicin as the active ingredient.

Il Thymus Vulgaris si esplicita in lingua italiana anche come timolo, essendo detto timolo l?olio essenziale di ti timo, essendo detto timolo presente in detta miscela con la quantit? percentuale compresa fra il 2% ed il 5% in peso di detta miscela, essendo detto timolo ricavato dalle foglie e dalle sommit? fiorite della pianta di timo. Thymus Vulgaris is also expressed in Italian as thymol, thymol being the essential oil of thymus, being said thymol present in said mixture with the quantity? percentage comprised between 2% and 5% by weight of said mixture, being said thymol obtained from the leaves and from the tops? flowers of the thyme plant.

Il Santalum Album si esplicita in lingua italiana anche come olio essenziale di Sandalo Citrino, essendo detto olio essenziale estratto dalla corteccia della pianta di sandalo citrino. Questo olio essenziale ? presente in detta miscela con la quantit? percentuale in peso compresa fra l?1% ed il 3% in peso di detta miscela. The Santalum Album is also expressed in Italian as essential oil of Citrine Sandalwood, being said essential oil extracted from the bark of the citrine sandalwood plant. This essential oil? present in said mixture with the quantity? percentage by weight comprised between 1% and 3% by weight of said mixture.

L?Origanum Vulgare si esplicita in lingua italiana anche come olio essenziale di origano, essendo il principio attivo di detto olio essenziale il carvacrolo, questo olio essenziale di origano essendo presente in detta miscela con la quantit? percentuale in peso compresa fra il 2% ed il 5% in peso di detta miscela. L? Origanum Vulgare is also explicit in Italian as essential oil of oregano, being the active principle of said essential oil the carvacrol, this essential oil of oregano being present in said mixture with the quantity? percentage by weight comprised between 2% and 5% by weight of said mixture.

Il Pelargonium Graveolens si esplicita in lingua italiana anche come olio essenziale di geranio, essendo detto olio essenziale presente in detta miscela con la quantit? percentuale in peso compresa fra il 2% ed il 6% in peso di detta miscela. The Pelargonium Graveolens is also explicit in the Italian language as essential oil of geranium, being said essential oil present in said mixture with the quantity? percentage by weight comprised between 2% and 6% by weight of said mixture.

La miscela oggetto della presente domanda di brevetto, oltre agli oli essenziali menzionati in precedenza, pu? contenere almeno uno degli oli essenziali compresi nell?insieme composto da: The mixture object of the present patent application, in addition to the essential oils mentioned above, can? contain at least one of the essential oils included in the set consisting of:

- Juniperus Communis, che si esplicita in lingua italiana anche come olio essenziale di ginepro; - Juniperus Communis, which is also expressed in Italian as essential oil of juniper;

- Cinnamomum Verum, oppure Cinnamomum Cassia, che si esplicita in lingua italiana anche come olio essenziale di cannella; - Cinnamomum Verum, or Cinnamomum Cassia, which is also expressed in Italian as essential oil of cinnamon;

- Eugenia Caryophyllus, che si esplicita in lingua italiana anche come olio essenziale di chiodi di garofano; - Eugenia Caryophyllus, which is also expressed in Italian as essential oil of cloves;

- Salvia Sclarea L, che si esplicita in lingua italiana anche come olio essenziale di salvia; - Salvia Sclarea L, which is also expressed in Italian as an essential oil of sage;

- Idrossi-tirosolo o 3,4 Diidrofeniletanolo, ovvero un alcol naturale proveniente dalle acque di vegetazione olearie, essendo dette acque relative al processo di lavorazione dell?olio d?oliva. - Hydroxy-tyrosol or 3,4 Dihydrophenylethanol, which is a natural alcohol coming from the waters of olive oil vegetation, being said waters relating to the olive oil processing process.

Di detto secondo gruppo di oli essenziali, ? possibile di utilizzarne uno o pi?, a scelta, per inserirlo nella prima miscela di oli essenziali, essendo detta possibilit? di utilizzo una opzione da attuare in relazione a particolari esigenze di trattamento di piante colpite dal batterio Xylella Fastidiosa con le sue varie sottospecie batteriche. Of said second group of essential oils,? It is possible to use one or more, of your choice, to insert it in the first blend of essential oils, being said possibility? of use an option to be implemented in relation to particular treatment needs of plants affected by the bacterium Xylella Fastidiosa with its various bacterial subspecies.

La presente invenzione ? pertanto relativa ad una composizione comprendente una miscela di oli essenziali come descritto. The present invention? therefore relating to a composition comprising a blend of essential oils as described.

Inoltre, l?invenzione ? relativa anche a un metodo per la lotta ai batteri che si caratterizza dal fatto di utilizzare una composizione secondo una o pi? delle rivendicazioni; in particolare, il metodo pu? essere utilizzato per la lotta al batterio Xylella Fastidiosa con le sue sottospecie batteriche. Furthermore, the invention? also related to a method for the fight against bacteria which is characterized by the fact of using a composition according to one or more? claims; in particular, the method can? be used to fight the Xylella Fastidiosa bacterium with its bacterial subspecies.

L?acqua ? presente in detta soluzione con una percentuale in peso variabile dal 20% al 70%, l?alcol etilico essendo presente in detta soluzione con una percentuale in peso variabile dal 30% all?80%. The water ? present in said solution with a weight percentage ranging from 20% to 70%, ethyl alcohol being present in said solution with a weight percentage ranging from 30% to 80%.

L?Alcol etilico ? presente in detta soluzione con una gradazione alcolica variabile da 40 a 80 gradi alcolici. L? Ethyl alcohol? present in said solution with an alcohol content ranging from 40 to 80 degrees alcohol.

In una sua preferibile forma di attuazione il metodo prevede di iniettare la soluzione idroalcolica dei detti oli essenziali in corrispondenza della base del tronco delle piante colpite. In one of its preferable embodiments, the method provides for injecting the hydroalcoholic solution of said essential oils at the base of the trunk of the affected plants.

Claims (10)

RIVENDICAZIONI 1) Una composizione per la lotta ai batteri caratterizzata dal fatto di essere costituita da una miscela di oli essenziali contenente: Allium Sativum, Thymus Vulgaris, Santalum Album, Origanum Vulgare, Pelargonium Graveolens, detti oli essenziali essendo diluiti con la proporzione di 1 a 10 rispetto ad una soluzione di acqua ed alcol. CLAIMS 1) A composition for the fight against bacteria characterized by the fact of being made up of a mixture of essential oils containing: Allium Sativum, Thymus Vulgaris, Santalum Album, Origanum Vulgare, Pelargonium Graveolens, said essential oils being diluted with the proportion of 1 to 10 compared to a solution of water and alcohol. 2) Composizione secondo la rivendicazione 1 caratterizzata dal fatto che detti oli essenziali sono ottenuti per macerazione delle piante corrispondenti essendo detti oli essenziali diluiti con una soluzione di acqua ed Alcol etilico. 2) Composition according to claim 1 characterized in that said essential oils are obtained by maceration of the corresponding plants, said essential oils being diluted with a solution of water and ethyl alcohol. 3) Composizione secondo la rivendicazione 2 caratterizzata dal fatto che l?acqua ? presente in detta soluzione con una percentuale in peso variabile dal 20% al 70%, l?alcol etilico essendo presente in detta soluzione con una percentuale in peso variabile dal 30% all?80%. 3) Composition according to claim 2 characterized in that water? present in said solution with a weight percentage ranging from 20% to 70%, ethyl alcohol being present in said solution with a weight percentage ranging from 30% to 80%. 4) Composizione secondo la rivendicazione 2 caratterizzata dal fatto che l?Alcol etilico ? presente in detta soluzione con una gradazione alcolica variabile da 40 a 80 gradi alcolici. 4) Composition according to claim 2 characterized in that ethyl alcohol? present in said solution with an alcohol content ranging from 40 to 80 degrees alcohol. 5) Composizione secondo una o pi? delle rivendicazioni precedenti caratterizzata dal fatto di essere utilizzata per la lotta al batterio Xylella Fastidiosa con le sue sottospecie batteriche. 5) Composition according to one or more? of the preceding claims characterized in that it is used to fight the Xylella Fastidiosa bacterium with its bacterial subspecies. 6) Composizione secondo una o pi? delle rivendicazioni precedenti caratterizzata dal fatto che la miscela comprende altres? almeno uno degli oli essenziali del gruppo comprendente: Juniperus Communis, Cinnamomum Verum o Cinnamomum Cassia, Eugenia Caryophyllus, Salvia Sclarea L. e Idrossi-tirosolo o 3,4 Diidrofeniletanolo. 6) Composition according to one or more? of the preceding claims characterized in that the mixture also comprises? at least one of the essential oils of the group comprising: Juniperus Communis, Cinnamomum Verum or Cinnamomum Cassia, Eugenia Caryophyllus, Salvia Sclarea L. and Hydroxy-tyrosol or 3,4 Dihydrophenylethanol. 7) Composizione secondo una o pi? delle rivendicazioni precedenti caratterizzata dal fatto che detto Allium Sativum ? presente in detta miscela con una percentuale in peso compresa fra 2% e 5%, detto Thymus Vulgaris ? presente in detta miscela con una percentuale in peso compresa fra 2% e 7%, detto Santalum Album ? presente in detta miscela con una percentuale in peso compresa fra 1% e 4%, detto Origanum Vulgare ? presente in detta miscela con una percentuale in peso compresa fra 2% e 5%, detto Pelargonium graveolens ? presente in detta miscela con una percentuale in peso compresa fra 2% e 5%. 7) Composition according to one or more? of the previous claims characterized by the fact that said Allium Sativum? present in said mixture with a percentage by weight between 2% and 5%, called Thymus Vulgaris? present in said blend with a weight percentage between 2% and 7%, called Santalum Album? present in said mixture with a percentage by weight between 1% and 4%, called Origanum Vulgare? present in said mixture with a percentage by weight between 2% and 5%, called Pelargonium graveolens? present in said mixture with a weight percentage between 2% and 5%. 8) Metodo per la lotta ai batteri caratterizzato dal fatto di utilizzare una composizione secondo una o pi? delle rivendicazioni precedenti. 8) Method for the fight against bacteria characterized by the fact of using a composition according to one or more? of the previous claims. 9) Metodo per la lotta al batterio Xylella Fastidiosa con le sue sottospecie batteriche caratterizzato dal fatto di utilizzare una composizione secondo una o pi? delle rivendicazioni da 1 a 8. 9) Method for the fight against the Xylella Fastidiosa bacterium with its bacterial subspecies characterized by the fact of using a composition according to one or more? of claims 1 to 8. 10) Metodo secondo la rivendicazione 9, caratterizzato dal fatto di iniettare la soluzione idroalcolica dei detti oli essenziali in corrispondenza della base del tronco delle piante colpite. 10) Method according to claim 9, characterized by injecting the hydroalcoholic solution of said essential oils at the base of the trunk of the affected plants.
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