IT1252628B - Formulazioni per matrici poliacrilamidiche in metodiche elettrocinetiche - Google Patents
Formulazioni per matrici poliacrilamidiche in metodiche elettrocineticheInfo
- Publication number
- IT1252628B IT1252628B ITMI913271A ITMI913271A IT1252628B IT 1252628 B IT1252628 B IT 1252628B IT MI913271 A ITMI913271 A IT MI913271A IT MI913271 A ITMI913271 A IT MI913271A IT 1252628 B IT1252628 B IT 1252628B
- Authority
- IT
- Italy
- Prior art keywords
- matrices
- monomers
- porosity
- polymerizing
- gel
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/26—Synthetic macromolecular compounds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
- C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
- C08F20/52—Amides or imides
- C08F20/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
- C08F20/58—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-acryloylmorpholine
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Dispersion Chemistry (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Polymerisation Methods In General (AREA)
Abstract
Matrici da impiegarsi in metodiche elettrocinetiche, a base di monomeri di acrilamide mono- e disostituite sull'azoto amidico (per esempio, dimetil acrilamide, acriloil-morfolina, trisacril) presentano i vantaggi a) di una spiccata resistenza all'idrolisi a pH alcalini (tipici dell'elettroforesi zonale); b) di una buona idrofilicità; c) di una più elevata porosità, dovuta all'aumentato Mr dei monomeri. E' inoltre possibile aumentare la porosità del gel polimerizzandolo in presenza di agenti di aggregazione laterale delle catene (per esempio, glicol polietilenico ad Mr 6.000-20.000 Da), con ottenimento di matrici che permettono una migliore risoluzione di proteine ad Mr elevato e di frammenti di acidi nucleici; polimerizzando la matrice in presenza di un gradiente di agente aggregante, si ottiene un gradiente di porosità anche con quantità costanti di monomeri nel gel. Infine, la fotopolimerizzazione (per esempio con riboflavina e luce) anziché con catalizzatori red/ox evita la formazione di N-ossidi di amine presenti nella soluzione di partenza.
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI913271A IT1252628B (it) | 1991-12-06 | 1991-12-06 | Formulazioni per matrici poliacrilamidiche in metodiche elettrocinetiche |
DE69225494T DE69225494T2 (de) | 1991-12-06 | 1992-08-05 | Hydrophile n-substitutierte polyacrylamid-gele und ihre verwendung in elektrokinetischen und chromatographischen methodologien |
PCT/EP1992/001772 WO1993011174A1 (en) | 1991-12-06 | 1992-08-05 | New formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies |
CA002125136A CA2125136C (en) | 1991-12-06 | 1992-08-05 | New formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies |
EP92916513A EP0615525B1 (en) | 1991-12-06 | 1992-08-05 | Hydrophilic poly-(n-substituted)acrylamide gels and their use in electrokinetic and chromatographic methodologies |
JP50973893A JP3313712B2 (ja) | 1991-12-06 | 1992-08-05 | 界面動電法およびクロマトグラフィー法におけるポリアクリルアミドマトリックスのための新規な配合物 |
AU23961/92A AU2396192A (en) | 1991-12-06 | 1992-08-05 | New formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies |
US08/244,608 US5470916A (en) | 1991-12-06 | 1992-08-05 | Formulations for polyacrylamide matrices in electrokinetic and chromatographic methodologies |
DE0615525T DE615525T1 (de) | 1991-12-06 | 1992-08-05 | Zusammensetzungen für polyacrylamid-matrizen in elektrokinetischen und chromatographischen methodologien. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITMI913271A IT1252628B (it) | 1991-12-06 | 1991-12-06 | Formulazioni per matrici poliacrilamidiche in metodiche elettrocinetiche |
Publications (3)
Publication Number | Publication Date |
---|---|
ITMI913271A0 ITMI913271A0 (it) | 1991-12-06 |
ITMI913271A1 ITMI913271A1 (it) | 1993-06-06 |
IT1252628B true IT1252628B (it) | 1995-06-19 |
Family
ID=11361272
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ITMI913271A IT1252628B (it) | 1991-12-06 | 1991-12-06 | Formulazioni per matrici poliacrilamidiche in metodiche elettrocinetiche |
Country Status (8)
Country | Link |
---|---|
US (1) | US5470916A (it) |
EP (1) | EP0615525B1 (it) |
JP (1) | JP3313712B2 (it) |
AU (1) | AU2396192A (it) |
CA (1) | CA2125136C (it) |
DE (2) | DE615525T1 (it) |
IT (1) | IT1252628B (it) |
WO (1) | WO1993011174A1 (it) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5627022A (en) * | 1994-11-01 | 1997-05-06 | Visible Genetics Inc. | Microgels for use in medical diagnosis and holders useful in fabricating same |
US5932285A (en) * | 1995-02-17 | 1999-08-03 | Medlogic Global Corporation | Encapsulated materials |
US5785832A (en) * | 1995-07-26 | 1998-07-28 | Chiari; Marcella | Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography |
IT1276957B1 (it) * | 1995-10-18 | 1997-11-03 | Pier Giorgio Righetti | Acrilamido derivati e formulazioni per matrici poliacrilamidiche in metodiche elettrocinetiche e cromatografiche |
US5840877A (en) * | 1996-09-05 | 1998-11-24 | Guest Elchrom Scientific | Electrophoresis gels of enhanced selectivity |
US6800278B1 (en) | 1996-10-28 | 2004-10-05 | Ballard Medical Products, Inc. | Inherently antimicrobial quaternary amine hydrogel wound dressings |
US6511803B1 (en) | 1997-10-10 | 2003-01-28 | President And Fellows Of Harvard College | Replica amplification of nucleic acid arrays |
WO1999038840A1 (en) * | 1998-01-30 | 1999-08-05 | Molecular Dynamics, Inc. | Compounds for molecular separations |
US6117293A (en) * | 1998-07-31 | 2000-09-12 | Biowhittaker Molecular Applications, Inc. | Method for producing hydrophilic monomers and uses thereof |
US6464850B1 (en) | 1998-07-31 | 2002-10-15 | Biowhittaker Molecular Applications, Inc. | Method for producing hydrophilic monomers and uses thereof |
US6410668B1 (en) | 1999-08-21 | 2002-06-25 | Marcella Chiari | Robust polymer coating |
US6410643B1 (en) | 2000-03-09 | 2002-06-25 | Surmodics, Inc. | Solid phase synthesis method and reagent |
DE10033583A1 (de) * | 2000-07-11 | 2002-01-24 | Bayer Ag | Superparamagnetische Perlpolymerisate |
SE0003358D0 (sv) * | 2000-09-19 | 2000-09-19 | Amersham Pharm Biotech Ab | Charged carrier material and its use |
AUPR522501A0 (en) * | 2001-05-25 | 2001-06-14 | Proteome Systems Ltd | Increased solubilisation of hydrophobic proteins |
ATE464559T1 (de) | 2002-10-13 | 2010-04-15 | Picosep As | Zweidimensionales mikrofluidisches system zur trennung von biomolekülen |
US20040140215A1 (en) * | 2003-01-17 | 2004-07-22 | Bio-Rad Laboratories, Inc. | Pre-cast electrophoresis slab gels with extended storage life |
WO2005029055A1 (en) * | 2003-09-19 | 2005-03-31 | Invitrogen Corporation | Composite compositions for electrophoresis |
DE502004005900D1 (de) | 2004-01-28 | 2008-02-21 | Mettler Toledo Gmbh | Polymer-Elektrolyt, Halbzelle für elektrochemische Messungen sowie deren Verwendung |
WO2005089911A1 (en) * | 2004-03-17 | 2005-09-29 | Ciphergen Biosystems, Inc. | Materials, methods, and systems for separating and identifying proteins from mixtures |
EP1907089B1 (en) * | 2005-07-28 | 2011-11-23 | Bio-Rad Laboratories, Inc. | Separation of proteins based on isoelectric point using solid-phase buffers |
US20070048249A1 (en) | 2005-08-24 | 2007-03-01 | Purdue Research Foundation | Hydrophilized bactericidal polymers |
GB0708921D0 (en) * | 2007-05-09 | 2007-06-20 | 3Lfants Ltd | Photo-initated, non aqueos, temperature independant gels, and method of preparation thereof |
CN103508913B (zh) * | 2012-06-27 | 2015-05-13 | 中国石油化工股份有限公司 | 单体和三元无规共聚物及其制备方法和三元无规共聚物的应用 |
JP6277806B2 (ja) | 2013-06-05 | 2018-02-14 | 株式会社リコー | インク |
US11285458B2 (en) * | 2013-07-08 | 2022-03-29 | National University Corporation Kyoto Institute Of Technology | Separating agent |
US20180361353A1 (en) * | 2015-12-25 | 2018-12-20 | Shinwa Chemical Industries Ltd. | Separating Agent for Chromatography, Chromatography Column, and Method for Separation by Chromatography |
CN111344562B (zh) | 2017-05-22 | 2023-09-12 | 因特根克斯股份有限公司 | 用于分子分析的组合物、方法、试剂盒和装置 |
WO2018217816A1 (en) | 2017-05-22 | 2018-11-29 | Integenx Inc. | Compositions, methods, kits and devices for molecular analysis |
WO2018222622A1 (en) | 2017-05-27 | 2018-12-06 | Poly Group LLC | Dispersible antimicrobial complex and coatings therefrom |
US20180362678A1 (en) | 2017-06-16 | 2018-12-20 | Poly Group LLC | Polymeric antimicrobial surfactant |
CN109633045A (zh) * | 2018-12-20 | 2019-04-16 | 广州广电计量检测股份有限公司 | 一种液相色谱串联质谱同时测定水产品中6种色素残留量的方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3037056A (en) * | 1959-03-30 | 1962-05-29 | California Research Corp | Amido polyglycols |
US3776956A (en) * | 1970-04-25 | 1973-12-04 | Lion Fat Oil Co Ltd | Resinous composition |
JPS50147844A (it) * | 1974-05-17 | 1975-11-27 | ||
JPS6147741A (ja) * | 1984-08-13 | 1986-03-08 | Nippon Synthetic Chem Ind Co Ltd:The | 硬化性樹脂組成物 |
JPS62159933A (ja) * | 1986-01-09 | 1987-07-15 | Nec Corp | フレ−ム同期検出回路 |
JP2578405B2 (ja) * | 1986-04-16 | 1997-02-05 | キヤノン株式会社 | デ−タ伝送システム |
US5055517A (en) * | 1988-04-29 | 1991-10-08 | At Biochem | Electrophoretic media |
EP0367886B1 (en) * | 1988-10-19 | 1995-04-19 | Guest Elchrom Scientific AG | Hydrophilic monomers, polymers thereof and their applications |
-
1991
- 1991-12-06 IT ITMI913271A patent/IT1252628B/it active IP Right Grant
-
1992
- 1992-08-05 JP JP50973893A patent/JP3313712B2/ja not_active Expired - Fee Related
- 1992-08-05 US US08/244,608 patent/US5470916A/en not_active Expired - Lifetime
- 1992-08-05 CA CA002125136A patent/CA2125136C/en not_active Expired - Lifetime
- 1992-08-05 AU AU23961/92A patent/AU2396192A/en not_active Abandoned
- 1992-08-05 DE DE0615525T patent/DE615525T1/de active Pending
- 1992-08-05 WO PCT/EP1992/001772 patent/WO1993011174A1/en active IP Right Grant
- 1992-08-05 EP EP92916513A patent/EP0615525B1/en not_active Expired - Lifetime
- 1992-08-05 DE DE69225494T patent/DE69225494T2/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP3313712B2 (ja) | 2002-08-12 |
EP0615525B1 (en) | 1998-05-13 |
JPH07501563A (ja) | 1995-02-16 |
EP0615525A1 (en) | 1994-09-21 |
DE615525T1 (de) | 1995-06-14 |
WO1993011174A1 (en) | 1993-06-10 |
DE69225494D1 (de) | 1998-06-18 |
AU2396192A (en) | 1993-06-28 |
CA2125136C (en) | 2003-12-30 |
CA2125136A1 (en) | 1993-06-10 |
ITMI913271A0 (it) | 1991-12-06 |
DE69225494T2 (de) | 1999-02-04 |
ITMI913271A1 (it) | 1993-06-06 |
US5470916A (en) | 1995-11-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
0001 | Granted | ||
TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19971223 |