IN2014DN09784A - - Google Patents

Info

Publication number
IN2014DN09784A
IN2014DN09784A IN9784DEN2014A IN2014DN09784A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A IN 9784DEN2014 A IN9784DEN2014 A IN 9784DEN2014A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A
Authority
IN
India
Prior art keywords
nucleic acid
target nucleic
sample
sample volumes
fractions
Prior art date
Application number
Other languages
English (en)
Inventor
Caifu Chen
Casey R Mcfarland
Jr David N Keys
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/032598 external-priority patent/WO2013158313A1/en
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of IN2014DN09784A publication Critical patent/IN2014DN09784A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38Temperature-responsive control
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IN9784DEN2014 2012-04-19 2014-11-19 IN2014DN09784A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261635584P 2012-04-19 2012-04-19
PCT/US2013/032598 WO2013158313A1 (en) 2012-04-19 2013-03-15 Nucleic acid amplification
PCT/US2013/037352 WO2013158982A1 (en) 2012-04-19 2013-04-19 Method of performing digital pcr

Publications (1)

Publication Number Publication Date
IN2014DN09784A true IN2014DN09784A (pt) 2015-07-31

Family

ID=49384101

Family Applications (1)

Application Number Title Priority Date Filing Date
IN9784DEN2014 IN2014DN09784A (pt) 2012-04-19 2014-11-19

Country Status (8)

Country Link
US (2) US10030262B2 (pt)
EP (1) EP2839029B1 (pt)
KR (1) KR20150011359A (pt)
CN (1) CN104487592B (pt)
AU (1) AU2013249123A1 (pt)
IN (1) IN2014DN09784A (pt)
RU (1) RU2014146286A (pt)
WO (1) WO2013158982A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017059049A1 (en) * 2015-09-29 2017-04-06 Life Technologies Corporation Systems and methods for performing digital pcr
CN108779423B (zh) * 2016-03-28 2021-12-21 富士胶片株式会社 Pcr用容器
WO2017196676A1 (en) * 2016-05-10 2017-11-16 Life Technologies Corporation Metal chelation post incorporation detection methods
EP3299471B1 (en) * 2016-09-23 2019-10-23 Roche Diagniostics GmbH Methods for determining the amount of a nucleic acid of interest in an unprocessed sample
BR112019012414A2 (pt) * 2016-12-19 2020-02-27 Quantum-Si Incorporated Carregamento de moléculas em poços de amostra para análise
CN106754312A (zh) * 2017-01-03 2017-05-31 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
CN106497774A (zh) * 2017-01-03 2017-03-15 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
US20180318839A1 (en) 2017-05-03 2018-11-08 Lifeos Genomics Corporation Incubation Device Having Rotary Mechanism
BR112019021889A2 (pt) 2017-05-05 2020-05-26 Quantum-Si Incorporated Substratos tendo reatividade de superfície modificada e propriedades anti-incrustantes em reações biológicas
JP6921638B2 (ja) 2017-06-16 2021-08-18 株式会社東芝 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム
CN109504745A (zh) * 2017-09-12 2019-03-22 莱弗斯基因股份有限公司 分析样品中多重目标核酸检测方法
CN107385103B (zh) * 2017-09-18 2023-04-07 星源智(珠海)生物科技有限公司 一种非纯化核酸扩增方法及装置
CN110819523B (zh) * 2018-08-13 2023-08-29 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN110819522B (zh) * 2018-08-13 2023-09-22 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN111349555B (zh) * 2018-12-21 2023-07-18 成都万众壹芯生物科技有限公司 一种基于微孔阵列芯片的数字pcr扩增装置及其使用方法
CN112239719A (zh) * 2019-07-19 2021-01-19 成都万众壹芯生物科技有限公司 基于微孔阵列芯片的数字pcr扩增装置和利用其进行扩增的方法
CA3157505A1 (en) 2019-10-11 2021-04-15 Quantum-Si Incorporated Surface modification in the vapor phase
US20220323958A1 (en) * 2019-12-04 2022-10-13 Hewlett-Packard Development Company, L.P. Sorting a droplet including a biologic sample
KR102395365B1 (ko) * 2020-02-10 2022-05-10 한국과학기술연구원 소수성 플레이트 상에 배열된 복수의 하이드로젤 미세입자를 포함하는 pcr용 반응 칩
CN111500408B (zh) * 2020-04-24 2022-03-22 浙江大学 在全封闭条件下进行核酸分析的试剂盒、装置及分析方法
WO2022109622A1 (en) * 2020-11-23 2022-05-27 Spangler Frank Leo Transparent heating elements for polymerase chain reaction devices
WO2022259182A1 (en) 2021-06-09 2022-12-15 Ecole Polytechnique Federale De Lausanne (Epfl) Device to carry out semiconductor-based diagnostics
KR102537348B1 (ko) * 2022-08-04 2023-05-30 한국기계연구원 디지털 pcr 분석용 기판 및 이의 제작방법

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
EP3540073B1 (en) 2004-06-01 2021-08-25 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
ES2923759T3 (es) * 2006-12-14 2022-09-30 Life Technologies Corp Aparato para medir analitos utilizando matrices de FET
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
DE602007008953D1 (de) * 2007-03-02 2010-10-14 Dna Electronics Ltd Qpcr unter verwendung eines ionen-sensitiven feldeffekt-transistors zur ph-messung
US20090053719A1 (en) * 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US20090286286A1 (en) 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
WO2009067632A1 (en) 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
EP2539464B1 (en) 2010-02-23 2016-11-16 Illumina, Inc. Amplification methods to minimise sequence specific bias
US20110262903A1 (en) * 2010-02-26 2011-10-27 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US9618475B2 (en) * 2010-09-15 2017-04-11 Life Technologies Corporation Methods and apparatus for measuring analytes
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen

Also Published As

Publication number Publication date
EP2839029B1 (en) 2017-11-15
US20180327813A1 (en) 2018-11-15
CN104487592B (zh) 2018-08-28
US20150099644A1 (en) 2015-04-09
EP2839029A1 (en) 2015-02-25
AU2013249123A1 (en) 2014-12-11
CN104487592A (zh) 2015-04-01
KR20150011359A (ko) 2015-01-30
RU2014146286A (ru) 2016-06-10
WO2013158982A1 (en) 2013-10-24
US10030262B2 (en) 2018-07-24

Similar Documents

Publication Publication Date Title
IN2014DN09784A (pt)
MX2015011575A (es) Sistemas y metodos para la deteccion de celulas usando particulas manejadas de transduccion.
IN2014KN02647A (pt)
SG10201908167YA (en) Proximity assays for detecting nucleic acids and proteins in a single cell
IL204288A (en) Methods and systems for determining the number of copies
IN2015DN01126A (pt)
WO2014028378A3 (en) Methods and systems for detecting biological components
EP3543362A3 (en) Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
SG10201804101XA (en) Multiple amplification cycle detection
NZ728582A (en) Instrument for analyzing biological samples and reagents
WO2012129363A3 (en) Single cell nucleic acid detection and analysis
WO2013050881A3 (en) Direct nucleic acid analysis
GB2479471A (en) Method for determining copy number variations
MX338296B (es) Composiciones y metodos para cuantificar una secuencia de acido nucleico en una muestra.
EA030186B9 (ru) Способ проведения количественных анализов
WO2012167142A3 (en) Enzyme quantification
GB201317477D0 (en) Methods, compositions and kits for determing the presence/absnece of a variant nucleic acid sequence
WO2014152281A3 (en) Ribosomal ribonucleic acid hybridization for organism identification
NZ628813A (en) Methods for predicting anti-cancer response
IN2014CN03508A (pt)
GB2515944A (en) Peptide arrays
MX2015001961A (es) Metodos y sistemas para ensayos.
IN2014KN01352A (pt)
WO2013177525A3 (en) Methods of nucleic acid fractionation and detection
EP2584045A4 (en) METHOD FOR DETECTING COLORECTAL TUMOR