IN2014DN09784A - - Google Patents

Info

Publication number
IN2014DN09784A
IN2014DN09784A IN9784DEN2014A IN2014DN09784A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A IN 9784DEN2014 A IN9784DEN2014 A IN 9784DEN2014A IN 2014DN09784 A IN2014DN09784 A IN 2014DN09784A
Authority
IN
India
Prior art keywords
nucleic acid
target nucleic
sample
sample volumes
fractions
Prior art date
Application number
Other languages
English (en)
Inventor
Caifu Chen
Casey R Mcfarland
Jr David N Keys
Original Assignee
Life Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2013/032598 external-priority patent/WO2013158313A1/fr
Application filed by Life Technologies Corp filed Critical Life Technologies Corp
Publication of IN2014DN09784A publication Critical patent/IN2014DN09784A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IN9784DEN2014 2012-04-19 2014-11-19 IN2014DN09784A (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261635584P 2012-04-19 2012-04-19
PCT/US2013/032598 WO2013158313A1 (fr) 2012-04-19 2013-03-15 Amplification d'acides nucléiques
PCT/US2013/037352 WO2013158982A1 (fr) 2012-04-19 2013-04-19 Procédé pour réaliser une pcr numérique

Publications (1)

Publication Number Publication Date
IN2014DN09784A true IN2014DN09784A (fr) 2015-07-31

Family

ID=49384101

Family Applications (1)

Application Number Title Priority Date Filing Date
IN9784DEN2014 IN2014DN09784A (fr) 2012-04-19 2014-11-19

Country Status (8)

Country Link
US (2) US10030262B2 (fr)
EP (1) EP2839029B1 (fr)
KR (1) KR20150011359A (fr)
CN (1) CN104487592B (fr)
AU (1) AU2013249123A1 (fr)
IN (1) IN2014DN09784A (fr)
RU (1) RU2014146286A (fr)
WO (1) WO2013158982A1 (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108368544B (zh) * 2015-09-29 2023-06-23 生命技术公司 用于执行数字pcr的系统和方法
CN108779423B (zh) * 2016-03-28 2021-12-21 富士胶片株式会社 Pcr用容器
WO2017196676A1 (fr) * 2016-05-10 2017-11-16 Life Technologies Corporation Procédés de détection de post-incorporation de métaux de chélation
EP3299471B1 (fr) * 2016-09-23 2019-10-23 Roche Diagniostics GmbH Procédés pour déterminer la quantité d'acide nucléique utile dans un échantillon non traité
MX2019007247A (es) * 2016-12-19 2019-08-16 Quantum Si Inc Carga de moleculas en pocillos para muestra para su analisis.
CN106754312A (zh) * 2017-01-03 2017-05-31 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
CN106497774A (zh) * 2017-01-03 2017-03-15 京东方科技集团股份有限公司 基因测序芯片、基因测序设备及基因测序方法
US20180318839A1 (en) 2017-05-03 2018-11-08 Lifeos Genomics Corporation Incubation Device Having Rotary Mechanism
AU2018261007A1 (en) 2017-05-05 2019-11-07 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
JP6921638B2 (ja) 2017-06-16 2021-08-18 株式会社東芝 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム
CN109504745A (zh) * 2017-09-12 2019-03-22 莱弗斯基因股份有限公司 分析样品中多重目标核酸检测方法
CN107385103B (zh) * 2017-09-18 2023-04-07 星源智（珠海）生物科技有限公司 一种非纯化核酸扩增方法及装置
CN110819523B (zh) * 2018-08-13 2023-08-29 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN110819522B (zh) * 2018-08-13 2023-09-22 上海新微技术研发中心有限公司 一种数字pcr系统及数字pcr液滴形成方法
CN111349555B (zh) * 2018-12-21 2023-07-18 成都万众壹芯生物科技有限公司 一种基于微孔阵列芯片的数字pcr扩增装置及其使用方法
CN112239719A (zh) * 2019-07-19 2021-01-19 成都万众壹芯生物科技有限公司 基于微孔阵列芯片的数字pcr扩增装置和利用其进行扩增的方法
BR112022006682A2 (pt) 2019-10-11 2022-07-12 Quantum Si Inc Modificação de superfície na fase de vapor
US20220323958A1 (en) * 2019-12-04 2022-10-13 Hewlett-Packard Development Company, L.P. Sorting a droplet including a biologic sample
KR102395365B1 (ko) * 2020-02-10 2022-05-10 한국과학기술연구원 소수성 플레이트 상에 배열된 복수의 하이드로젤 미세입자를 포함하는 pcr용 반응 칩
CN111500408B (zh) * 2020-04-24 2022-03-22 浙江大学 在全封闭条件下进行核酸分析的试剂盒、装置及分析方法
US20220161264A1 (en) * 2020-11-23 2022-05-26 Frank Leo Spangler Transparent heating elements for polymerase chain reaction devices
WO2022259182A1 (fr) 2021-06-09 2022-12-15 Ecole Polytechnique Federale De Lausanne (Epfl) Dispositif de réalisation de diagnostics à base de semi-conducteurs
KR102537348B1 (ko) * 2022-08-04 2023-05-30 한국기계연구원 디지털 pcr 분석용 기판 및 이의 제작방법

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
AU2005250233B2 (en) 2004-06-01 2011-03-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
WO2007107710A1 (fr) 2006-03-17 2007-09-27 Solexa Limited Procédés isothermiques pour créer des réseaux moléculaires clonales simples
EP2674751B1 (fr) 2006-12-14 2017-02-01 Life Technologies Corporation Appareil de mesure d'analytes à l'aide de matrices de FET à grande échelle
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
CN101743319B (zh) * 2007-03-02 2013-03-06 Dna电子有限公司 使用固态pH传感器的qPCR
US20090053719A1 (en) * 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US20090286286A1 (en) 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
US20090171078A1 (en) 2007-11-20 2009-07-02 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorotiolate compounds
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8759037B2 (en) 2010-02-23 2014-06-24 Illumina Cambridge Limited Amplification methods to minimise sequence specific bias
US20110318748A1 (en) 2010-02-26 2011-12-29 Life Technologies Corporation Modified Proteins and Methods of Making and Using Same
US9618475B2 (en) * 2010-09-15 2017-04-11 Life Technologies Corporation Methods and apparatus for measuring analytes
EP3564392B1 (fr) 2010-12-17 2021-11-24 Life Technologies Corporation Procédés pour l'amplification d'acides nucléiques
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen

Also Published As

Publication number Publication date
US20150099644A1 (en) 2015-04-09
US20180327813A1 (en) 2018-11-15
CN104487592A (zh) 2015-04-01
AU2013249123A1 (en) 2014-12-11
US10030262B2 (en) 2018-07-24
WO2013158982A1 (fr) 2013-10-24
KR20150011359A (ko) 2015-01-30
EP2839029B1 (fr) 2017-11-15
RU2014146286A (ru) 2016-06-10
CN104487592B (zh) 2018-08-28
EP2839029A1 (fr) 2015-02-25

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