IL43343A - Production of plasminogen activator - Google Patents
Production of plasminogen activatorInfo
- Publication number
- IL43343A IL43343A IL43343A IL4334373A IL43343A IL 43343 A IL43343 A IL 43343A IL 43343 A IL43343 A IL 43343A IL 4334373 A IL4334373 A IL 4334373A IL 43343 A IL43343 A IL 43343A
- Authority
- IL
- Israel
- Prior art keywords
- plasminogen activator
- mammalian cells
- medium
- atcc
- buffer
- Prior art date
Links
- 102000001938 Plasminogen Activators Human genes 0.000 title claims 24
- 108010001014 Plasminogen Activators Proteins 0.000 title claims 24
- 229940127126 plasminogen activator Drugs 0.000 title claims 24
- 238000000034 method Methods 0.000 claims 19
- 210000004962 mammalian cell Anatomy 0.000 claims 10
- 210000003292 kidney cell Anatomy 0.000 claims 7
- 239000002609 medium Substances 0.000 claims 7
- 150000003839 salts Chemical class 0.000 claims 6
- 229930013930 alkaloid Natural products 0.000 claims 5
- 150000003797 alkaloid derivatives Chemical class 0.000 claims 5
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 4
- 239000000872 buffer Substances 0.000 claims 4
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 4
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 4
- 239000003104 tissue culture media Substances 0.000 claims 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims 3
- 235000015097 nutrients Nutrition 0.000 claims 3
- 239000008363 phosphate buffer Substances 0.000 claims 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- 241000282560 Macaca mulatta Species 0.000 claims 2
- JXLYSJRDGCGARV-WWYNWVTFSA-N Vinblastine Natural products O=C(O[C@H]1[C@](O)(C(=O)OC)[C@@H]2N(C)c3c(cc(c(OC)c3)[C@]3(C(=O)OC)c4[nH]c5c(c4CCN4C[C@](O)(CC)C[C@H](C3)C4)cccc5)[C@@]32[C@H]2[C@@]1(CC)C=CCN2CC3)C JXLYSJRDGCGARV-WWYNWVTFSA-N 0.000 claims 2
- 239000003463 adsorbent Substances 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- -1 colchamide Chemical compound 0.000 claims 2
- 229960001338 colchicine Drugs 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000001103 potassium chloride Substances 0.000 claims 2
- 235000011164 potassium chloride Nutrition 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 2
- 229960003048 vinblastine Drugs 0.000 claims 2
- JXLYSJRDGCGARV-XQKSVPLYSA-N vincaleukoblastine Chemical compound C([C@@H](C[C@]1(C(=O)OC)C=2C(=CC3=C([C@]45[C@H]([C@@]([C@H](OC(C)=O)[C@]6(CC)C=CCN([C@H]56)CC4)(O)C(=O)OC)N3C)C=2)OC)C[C@@](C2)(O)CC)N2CCC2=C1NC1=CC=CC=C21 JXLYSJRDGCGARV-XQKSVPLYSA-N 0.000 claims 2
- 229960004528 vincristine Drugs 0.000 claims 2
- OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 claims 2
- OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 claims 2
- IPDJRYUVROTJTO-UHFFFAOYSA-N 1,2,3,10-tetramethoxy-6,7-dihydro-5h-benzo[a]heptalen-9-one Chemical compound C1CCC2=CC(=O)C(OC)=CC=C2C2=C1C=C(OC)C(OC)=C2OC IPDJRYUVROTJTO-UHFFFAOYSA-N 0.000 claims 1
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- HFPMXDMZJUJZBX-AWEZNQCLSA-N Deacetylcolchicine Chemical compound C1([C@@H](N)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC HFPMXDMZJUJZBX-AWEZNQCLSA-N 0.000 claims 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 claims 1
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 1
- 229920005654 Sephadex Polymers 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical group N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000006285 cell suspension Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 239000012153 distilled water Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 239000002244 precipitate Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30005472A | 1972-10-24 | 1972-10-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL43343A0 IL43343A0 (en) | 1973-11-28 |
| IL43343A true IL43343A (en) | 1976-03-31 |
Family
ID=23157506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL43343A IL43343A (en) | 1972-10-24 | 1973-09-30 | Production of plasminogen activator |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JPS4975794A (en)van) |
| BE (1) | BE806323A (en)van) |
| CH (1) | CH589142A5 (en)van) |
| DE (1) | DE2353318A1 (en)van) |
| FR (1) | FR2203876B1 (en)van) |
| GB (1) | GB1443189A (en)van) |
| IL (1) | IL43343A (en)van) |
| NL (1) | NL7314579A (en)van) |
| SE (2) | SE392618B (en)van) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5571496A (en) * | 1978-11-24 | 1980-05-29 | Asahi Chem Ind Co Ltd | Preparation of living substance |
| FI831484A7 (fi) | 1982-05-05 | 1983-11-06 | Genentech Inc | Ihmiskudoksen plasminogeenien aktivaattori. |
| SU1662352A3 (ru) * | 1982-05-05 | 1991-07-07 | Генентек, Инк (Фирма) | Штамм бактерий ЕSснеRIснIа coLI - продуцент активатора плазминогена тканевого типа |
| US4853330A (en) * | 1983-04-07 | 1989-08-01 | Genentech, Inc. | Human tissue plasminogen activator |
| US5185259A (en) * | 1982-05-05 | 1993-02-09 | Genentech, Inc. | Truncated human tissue plasminogen activator |
| IL68561A (en) * | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
| IL69664A0 (en) * | 1982-09-15 | 1983-12-30 | Collaborative Res Inc | Medium supplement and method for growing cells in vitro |
| NO164991C (no) * | 1984-09-21 | 1990-12-05 | Boehringer Ingelheim Int | Fremgangsmaate for fremstilling av blodkoaguleringshemmende proteiner. |
| JP2507339B2 (ja) * | 1986-08-11 | 1996-06-12 | 三井東圧化学株式会社 | 粗ｔＰＡの精製方法 |
-
1973
- 1973-09-30 IL IL43343A patent/IL43343A/en unknown
- 1973-10-19 FR FR7337349A patent/FR2203876B1/fr not_active Expired
- 1973-10-22 CH CH1484773A patent/CH589142A5/xx not_active IP Right Cessation
- 1973-10-22 BE BE1005443A patent/BE806323A/xx unknown
- 1973-10-23 GB GB4919673A patent/GB1443189A/en not_active Expired
- 1973-10-23 NL NL7314579A patent/NL7314579A/xx not_active Application Discontinuation
- 1973-10-23 SE SE7314381A patent/SE392618B/xx unknown
- 1973-10-24 JP JP48119815A patent/JPS4975794A/ja active Pending
- 1973-10-24 DE DE19732353318 patent/DE2353318A1/de active Pending
-
1976
- 1976-09-07 SE SE7609880A patent/SE7609880L/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL43343A0 (en) | 1973-11-28 |
| CH589142A5 (en)van) | 1977-06-30 |
| FR2203876A1 (en)van) | 1974-05-17 |
| BE806323A (fr) | 1974-04-22 |
| FR2203876B1 (en)van) | 1977-05-27 |
| DE2353318A1 (de) | 1974-05-02 |
| NL7314579A (en)van) | 1974-04-26 |
| SE392618B (sv) | 1977-04-04 |
| JPS4975794A (en)van) | 1974-07-22 |
| GB1443189A (en) | 1976-07-21 |
| SE7609880L (sv) | 1976-09-07 |
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