IL297108A - A method for purifying single-stranded RNA - Google Patents
A method for purifying single-stranded RNAInfo
- Publication number
- IL297108A IL297108A IL297108A IL29710822A IL297108A IL 297108 A IL297108 A IL 297108A IL 297108 A IL297108 A IL 297108A IL 29710822 A IL29710822 A IL 29710822A IL 297108 A IL297108 A IL 297108A
- Authority
- IL
- Israel
- Prior art keywords
- pyrophosphate
- ssrna
- solid phase
- stranded rna
- sample
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20170191.9A EP3896160B1 (en) | 2020-04-17 | 2020-04-17 | A method of single-stranded rna purification |
| PCT/EP2021/059906 WO2021209597A1 (en) | 2020-04-17 | 2021-04-16 | A method of single-stranded rna purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL297108A true IL297108A (en) | 2022-12-01 |
Family
ID=70333782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL297108A IL297108A (en) | 2020-04-17 | 2021-04-16 | A method for purifying single-stranded RNA |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US12522816B2 (https=) |
| EP (1) | EP3896160B1 (https=) |
| JP (1) | JP7746285B2 (https=) |
| KR (1) | KR20230025770A (https=) |
| CN (1) | CN115768887A (https=) |
| CA (1) | CA3177259A1 (https=) |
| DK (1) | DK3896160T3 (https=) |
| ES (1) | ES3034521T3 (https=) |
| IL (1) | IL297108A (https=) |
| SI (1) | SI3896160T1 (https=) |
| WO (1) | WO2021209597A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20250257385A1 (en) * | 2022-10-31 | 2025-08-14 | Genemind Biosciences Co., Ltd. | Surface treatment method, sequencing method, and kit |
| CN116218838A (zh) * | 2023-01-28 | 2023-06-06 | 翌圣生物科技(上海)股份有限公司 | 从阴离子交换树脂洗脱长链核酸rna的方法 |
| CN119345744A (zh) * | 2024-05-10 | 2025-01-24 | 上海渔霁生物技术有限公司 | 一种弱阴离子改性的整体柱 |
| KR20250173969A (ko) * | 2024-06-04 | 2025-12-11 | 주식회사 녹십자 | 단일가닥 rna 정제 방법 |
| CN120290548B (zh) * | 2025-06-12 | 2025-09-23 | 硅羿科技（上海）有限公司 | 一种dsRNA标准品的纯化和制备方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| EP2250182B1 (en) * | 2008-02-07 | 2015-12-02 | GE Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| US10590161B2 (en) | 2013-03-15 | 2020-03-17 | Modernatx, Inc. | Ion exchange purification of mRNA |
| BR112016000160B1 (pt) | 2013-07-05 | 2021-06-08 | Bioneer Corporation | estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma |
| SI3445850T1 (sl) * | 2016-04-22 | 2021-12-31 | BioNTech SE | Postopki za zagotavljanje enoverižne RNA |
| WO2018096179A1 (en) * | 2016-11-28 | 2018-05-31 | Curevac Ag | Method for purifying rna |
-
2020
- 2020-04-17 EP EP20170191.9A patent/EP3896160B1/en active Active
- 2020-04-17 SI SI202030635T patent/SI3896160T1/sl unknown
- 2020-04-17 ES ES20170191T patent/ES3034521T3/es active Active
- 2020-04-17 DK DK20170191.9T patent/DK3896160T3/da active
-
2021
- 2021-04-16 CN CN202180028161.XA patent/CN115768887A/zh active Pending
- 2021-04-16 IL IL297108A patent/IL297108A/en unknown
- 2021-04-16 CA CA3177259A patent/CA3177259A1/en active Pending
- 2021-04-16 WO PCT/EP2021/059906 patent/WO2021209597A1/en not_active Ceased
- 2021-04-16 JP JP2022563099A patent/JP7746285B2/ja active Active
- 2021-04-16 US US17/918,503 patent/US12522816B2/en active Active
- 2021-04-16 KR KR1020227035731A patent/KR20230025770A/ko active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20230142600A1 (en) | 2023-05-11 |
| SI3896160T1 (sl) | 2025-08-29 |
| EP3896160A1 (en) | 2021-10-20 |
| US12522816B2 (en) | 2026-01-13 |
| KR20230025770A (ko) | 2023-02-23 |
| WO2021209597A1 (en) | 2021-10-21 |
| ES3034521T3 (en) | 2025-08-19 |
| DK3896160T3 (da) | 2025-07-07 |
| JP7746285B2 (ja) | 2025-09-30 |
| EP3896160B1 (en) | 2025-06-04 |
| JP2023521504A (ja) | 2023-05-24 |
| CN115768887A (zh) | 2023-03-07 |
| CA3177259A1 (en) | 2021-10-21 |
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