JP7746285B2 - 一本鎖ｒｎａの精製方法 - Google Patents

一本鎖ｒｎａの精製方法

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Publication number
JP7746285B2
JP7746285B2 JP2022563099A JP2022563099A JP7746285B2 JP 7746285 B2 JP7746285 B2 JP 7746285B2 JP 2022563099 A JP2022563099 A JP 2022563099A JP 2022563099 A JP2022563099 A JP 2022563099A JP 7746285 B2 JP7746285 B2 JP 7746285B2
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Prior art keywords
pyrophosphate
solid phase
ssrna
stranded rna
sample
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JP2022563099A
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Japanese (ja)
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JP2023521504A (ja
JP2023521504A5 (https=
Inventor
ペルシッチ、スペラ
チェルニゴイ、ウルフ
ドレンツ、ダルコ
スタンリー ギャニオン、ピーター
Original Assignee
ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
JP2022563099A 2020-04-17 2021-04-16 一本鎖ｒｎａの精製方法 Active JP7746285B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170191.9A EP3896160B1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
EP20170191.9 2020-04-17
PCT/EP2021/059906 WO2021209597A1 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (3)

Publication Number Publication Date
JP2023521504A JP2023521504A (ja) 2023-05-24
JP2023521504A5 JP2023521504A5 (https=) 2024-04-23
JP7746285B2 true JP7746285B2 (ja) 2025-09-30

Family

ID=70333782

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022563099A Active JP7746285B2 (ja) 2020-04-17 2021-04-16 一本鎖ｒｎａの精製方法

Country Status (11)

Country Link
US (1) US12522816B2 (https=)
EP (1) EP3896160B1 (https=)
JP (1) JP7746285B2 (https=)
KR (1) KR20230025770A (https=)
CN (1) CN115768887A (https=)
CA (1) CA3177259A1 (https=)
DK (1) DK3896160T3 (https=)
ES (1) ES3034521T3 (https=)
IL (1) IL297108A (https=)
SI (1) SI3896160T1 (https=)
WO (1) WO2021209597A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250257385A1 (en) * 2022-10-31 2025-08-14 Genemind Biosciences Co., Ltd. Surface treatment method, sequencing method, and kit
CN116218838A (zh) * 2023-01-28 2023-06-06 翌圣生物科技(上海)股份有限公司 从阴离子交换树脂洗脱长链核酸rna的方法
CN119345744A (zh) * 2024-05-10 2025-01-24 上海渔霁生物技术有限公司 一种弱阴离子改性的整体柱
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
JP2016530875A (ja) 2013-07-05 2016-10-06 バイオニア コーポレーションＢｉｏｎｅｅｒ Ｃｏｒｐｏｒａｔｉｏｎ 改善された高効率ナノ粒子型オリゴヌクレオチド構造体およびその製造方法
WO2017182524A1 (en) 2016-04-22 2017-10-26 Biontech Rna Pharmaceuticals Gmbh Methods for providing single-stranded rna
WO2018096179A1 (en) 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
JP2016530875A (ja) 2013-07-05 2016-10-06 バイオニア コーポレーションＢｉｏｎｅｅｒ Ｃｏｒｐｏｒａｔｉｏｎ 改善された高効率ナノ粒子型オリゴヌクレオチド構造体およびその製造方法
WO2017182524A1 (en) 2016-04-22 2017-10-26 Biontech Rna Pharmaceuticals Gmbh Methods for providing single-stranded rna
WO2018096179A1 (en) 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Chemistry of Natural Compounds，1992年，Vol.28，p.127-128
Journal of Chromatography A，2013年，Vol.1278，p.54-60
Microbes Environ.，2015年，Vol.30, No.2，p.199-203
Molecular Therapy: Nucleic Acids，2019年，Vol.15，p.26-35

Also Published As

Publication number Publication date
US20230142600A1 (en) 2023-05-11
SI3896160T1 (sl) 2025-08-29
EP3896160A1 (en) 2021-10-20
US12522816B2 (en) 2026-01-13
IL297108A (en) 2022-12-01
KR20230025770A (ko) 2023-02-23
WO2021209597A1 (en) 2021-10-21
ES3034521T3 (en) 2025-08-19
DK3896160T3 (da) 2025-07-07
EP3896160B1 (en) 2025-06-04
JP2023521504A (ja) 2023-05-24
CN115768887A (zh) 2023-03-07
CA3177259A1 (en) 2021-10-21

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