CA3177259A1 - A method of single-stranded rna purification - Google Patents

A method of single-stranded rna purification

Info

Publication number
CA3177259A1
CA3177259A1 CA3177259A CA3177259A CA3177259A1 CA 3177259 A1 CA3177259 A1 CA 3177259A1 CA 3177259 A CA3177259 A CA 3177259A CA 3177259 A CA3177259 A CA 3177259A CA 3177259 A1 CA3177259 A1 CA 3177259A1
Authority
CA
Canada
Prior art keywords
stranded rna
rna purification
solid phase
solid
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3177259A
Other languages
English (en)
French (fr)
Inventor
Spela Persic Debenjak
Urh Cernigoj
Darko DOLENC
Peter Stanley GAGNON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Bia Separations doo
Original Assignee
Sartorius Bia Separations doo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Bia Separations doo filed Critical Sartorius Bia Separations doo
Publication of CA3177259A1 publication Critical patent/CA3177259A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CA3177259A 2020-04-17 2021-04-16 A method of single-stranded rna purification Pending CA3177259A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170191.9A EP3896160B1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
EP20170191.9 2020-04-17
PCT/EP2021/059906 WO2021209597A1 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (1)

Publication Number Publication Date
CA3177259A1 true CA3177259A1 (en) 2021-10-21

Family

ID=70333782

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3177259A Pending CA3177259A1 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Country Status (11)

Country Link
US (1) US12522816B2 (https=)
EP (1) EP3896160B1 (https=)
JP (1) JP7746285B2 (https=)
KR (1) KR20230025770A (https=)
CN (1) CN115768887A (https=)
CA (1) CA3177259A1 (https=)
DK (1) DK3896160T3 (https=)
ES (1) ES3034521T3 (https=)
IL (1) IL297108A (https=)
SI (1) SI3896160T1 (https=)
WO (1) WO2021209597A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250257385A1 (en) * 2022-10-31 2025-08-14 Genemind Biosciences Co., Ltd. Surface treatment method, sequencing method, and kit
CN116218838A (zh) * 2023-01-28 2023-06-06 翌圣生物科技(上海)股份有限公司 从阴离子交换树脂洗脱长链核酸rna的方法
CN119345744A (zh) * 2024-05-10 2025-01-24 上海渔霁生物技术有限公司 一种弱阴离子改性的整体柱
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법
CN120290548B (zh) * 2025-06-12 2025-09-23 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Also Published As

Publication number Publication date
US20230142600A1 (en) 2023-05-11
SI3896160T1 (sl) 2025-08-29
EP3896160A1 (en) 2021-10-20
US12522816B2 (en) 2026-01-13
IL297108A (en) 2022-12-01
KR20230025770A (ko) 2023-02-23
WO2021209597A1 (en) 2021-10-21
ES3034521T3 (en) 2025-08-19
DK3896160T3 (da) 2025-07-07
JP7746285B2 (ja) 2025-09-30
EP3896160B1 (en) 2025-06-04
JP2023521504A (ja) 2023-05-24
CN115768887A (zh) 2023-03-07

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