CN115768887A - 一种单链rna纯化方法 - Google Patents

一种单链rna纯化方法 Download PDF

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Publication number
CN115768887A
CN115768887A CN202180028161.XA CN202180028161A CN115768887A CN 115768887 A CN115768887 A CN 115768887A CN 202180028161 A CN202180028161 A CN 202180028161A CN 115768887 A CN115768887 A CN 115768887A
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China
Prior art keywords
pyrophosphate
stranded rna
ssrna
solid phase
sample
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Pending
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CN202180028161.XA
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English (en)
Chinese (zh)
Inventor
S·佩尔西奇
U·塞尼戈吉
D·多伦茨
P·S·加农
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Sedolis Biya Separation Co ltd
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Sedolis Biya Separation Co ltd
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Publication of CN115768887A publication Critical patent/CN115768887A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN202180028161.XA 2020-04-17 2021-04-16 一种单链rna纯化方法 Pending CN115768887A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20170191.9A EP3896160B1 (en) 2020-04-17 2020-04-17 A method of single-stranded rna purification
EP20170191.9 2020-04-17
PCT/EP2021/059906 WO2021209597A1 (en) 2020-04-17 2021-04-16 A method of single-stranded rna purification

Publications (1)

Publication Number Publication Date
CN115768887A true CN115768887A (zh) 2023-03-07

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ID=70333782

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180028161.XA Pending CN115768887A (zh) 2020-04-17 2021-04-16 一种单链rna纯化方法

Country Status (11)

Country Link
US (1) US12522816B2 (https=)
EP (1) EP3896160B1 (https=)
JP (1) JP7746285B2 (https=)
KR (1) KR20230025770A (https=)
CN (1) CN115768887A (https=)
CA (1) CA3177259A1 (https=)
DK (1) DK3896160T3 (https=)
ES (1) ES3034521T3 (https=)
IL (1) IL297108A (https=)
SI (1) SI3896160T1 (https=)
WO (1) WO2021209597A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120290548A (zh) * 2025-06-12 2025-07-11 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法
WO2025232847A1 (zh) * 2024-05-10 2025-11-13 上海渔霁生物技术有限公司 一种弱阴离子改性的整体柱

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250257385A1 (en) * 2022-10-31 2025-08-14 Genemind Biosciences Co., Ltd. Surface treatment method, sequencing method, and kit
CN116218838A (zh) * 2023-01-28 2023-06-06 翌圣生物科技(上海)股份有限公司 从阴离子交换树脂洗脱长链核酸rna的方法
KR20250173969A (ko) * 2024-06-04 2025-12-11 주식회사 녹십자 단일가닥 rna 정제 방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
EP2250182B1 (en) * 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
US10590161B2 (en) 2013-03-15 2020-03-17 Modernatx, Inc. Ion exchange purification of mRNA
BR112016000160B1 (pt) 2013-07-05 2021-06-08 Bioneer Corporation estrutura de oligonucleotídeo tipo nanopartícula melhorada tendo alta eficiência e método para a preparação da mesma
SI3445850T1 (sl) * 2016-04-22 2021-12-31 BioNTech SE Postopki za zagotavljanje enoverižne RNA
WO2018096179A1 (en) * 2016-11-28 2018-05-31 Curevac Ag Method for purifying rna

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025232847A1 (zh) * 2024-05-10 2025-11-13 上海渔霁生物技术有限公司 一种弱阴离子改性的整体柱
CN120290548A (zh) * 2025-06-12 2025-07-11 硅羿科技（上海）有限公司 一种dsRNA标准品的纯化和制备方法

Also Published As

Publication number Publication date
US20230142600A1 (en) 2023-05-11
SI3896160T1 (sl) 2025-08-29
EP3896160A1 (en) 2021-10-20
US12522816B2 (en) 2026-01-13
IL297108A (en) 2022-12-01
KR20230025770A (ko) 2023-02-23
WO2021209597A1 (en) 2021-10-21
ES3034521T3 (en) 2025-08-19
DK3896160T3 (da) 2025-07-07
JP7746285B2 (ja) 2025-09-30
EP3896160B1 (en) 2025-06-04
JP2023521504A (ja) 2023-05-24
CA3177259A1 (en) 2021-10-21

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Legal Events

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CB03 Change of inventor or designer information

Inventor after: S. Persychid Benzac

Inventor after: U. Senigogi

Inventor after: D. Dorenz

Inventor after: GAGNON PETER S

Inventor before: S. Persich

Inventor before: U. Senigogi

Inventor before: D. Dorenz

Inventor before: GAGNON PETER S

CB03 Change of inventor or designer information