IL296604A - Methods and compositions for preparing nucleic acid libraries - Google Patents
Methods and compositions for preparing nucleic acid librariesInfo
- Publication number
- IL296604A IL296604A IL296604A IL29660422A IL296604A IL 296604 A IL296604 A IL 296604A IL 296604 A IL296604 A IL 296604A IL 29660422 A IL29660422 A IL 29660422A IL 296604 A IL296604 A IL 296604A
- Authority
- IL
- Israel
- Prior art keywords
- extension
- amplification
- nucleic acids
- target nucleic
- sites
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/143—Concentration of primer or probe
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063001684P | 2020-03-30 | 2020-03-30 | |
| PCT/US2021/024695 WO2021202403A1 (en) | 2020-03-30 | 2021-03-29 | Methods and compositions for preparing nucleic acid libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL296604A true IL296604A (en) | 2022-11-01 |
Family
ID=75581670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL296604A IL296604A (en) | 2020-03-30 | 2021-03-29 | Methods and compositions for preparing nucleic acid libraries |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20230105642A1 (https=) |
| EP (2) | EP4421187A3 (https=) |
| JP (1) | JP2023520203A (https=) |
| KR (1) | KR20220160661A (https=) |
| CN (1) | CN115698319A (https=) |
| AU (1) | AU2021249046A1 (https=) |
| BR (1) | BR112022019541A2 (https=) |
| CA (1) | CA3176615A1 (https=) |
| ES (1) | ES2985378T3 (https=) |
| IL (1) | IL296604A (https=) |
| MX (1) | MX2022012184A (https=) |
| WO (1) | WO2021202403A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023077029A2 (en) * | 2021-10-27 | 2023-05-04 | Mission Bio, Inc. | Single cell viral integration site detection |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
| AU3539089A (en) | 1988-04-08 | 1989-11-03 | Salk Institute For Biological Studies, The | Ligase-based amplification method |
| ATE144556T1 (de) | 1988-06-24 | 1996-11-15 | Amgen Inc | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| ATE138106T1 (de) | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| CA2035010C (en) | 1990-01-26 | 1996-12-10 | Keith C. Backman | Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| WO1995021271A1 (en) | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| WO1995025180A1 (en) | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| WO1997014028A2 (en) | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| DE69812329T2 (de) | 1997-11-18 | 2004-02-12 | Bio-Rad Laboratories, Inc., Hercules | Multiplex-zufluss-immunotest mit magnetischen teilchen als festphase |
| EP1204869B1 (en) | 1999-08-17 | 2008-10-22 | Luminex Corporation | Method for analyzing a number of samples from a variety of sources for a single analyte |
| WO2001013119A1 (en) | 1999-08-17 | 2001-02-22 | Luminex Corporation | Encapsulation of fluorescent particles |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| CA2498764C (en) | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| EP1704402B1 (en) | 2004-01-14 | 2016-05-11 | Luminex Corporation | Methods and systems for dynamic range expansion |
| CA2579150C (en) | 2004-09-17 | 2014-11-25 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| WO2006079016A1 (en) | 2005-01-20 | 2006-07-27 | Luminex Corporation | Magnetic microspheres for use in fluorescence-based applications |
| EP4071458B1 (en) | 2005-09-21 | 2025-11-05 | Luminex Corporation | Methods and systems for image data processing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| US20070207513A1 (en) | 2006-03-03 | 2007-09-06 | Luminex Corporation | Methods, Products, and Kits for Identifying an Analyte in a Sample |
| CN102851369B (zh) | 2006-12-13 | 2015-01-21 | 卢米耐克斯公司 | 用于实时pcr多重分析的系统和方法 |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| NZ630698A (en) * | 2012-09-25 | 2017-03-31 | Agriculture Victoria Serv Pty | Method of producing a normalised nucleic acid library using solid state capture material |
| KR102001554B1 (ko) | 2014-01-16 | 2019-07-18 | 일루미나, 인코포레이티드 | 고형 지지체 상에서의 앰플리콘 제조 방법 및 시퀀싱 |
| CN106574266A (zh) * | 2014-08-14 | 2017-04-19 | 雅培分子公司 | 用于下一代测序的文库生成 |
| DK3234187T3 (da) | 2014-12-15 | 2021-05-25 | Illumina Inc | Fremgangsmåde til enkeltmolekyleanbringelse på et substrat |
| EP3440220B1 (en) * | 2016-04-07 | 2020-03-25 | Illumina, Inc. | Methods and systems for construction of normalized nucleic acid libraries |
| KR102866805B1 (ko) * | 2017-02-21 | 2025-09-30 | 일루미나, 인코포레이티드 | 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화 |
| CN110079592B (zh) * | 2018-01-26 | 2021-02-12 | 厦门艾德生物医药科技股份有限公司 | 用于检测基因突变和已知、未知基因融合类型的高通量测序靶向捕获目标区域的探针和方法 |
| SG11202012762WA (en) | 2018-12-05 | 2021-01-28 | Illumina Cambridge Ltd | Methods and compositions for cluster generation by bridge amplification |
-
2021
- 2021-03-29 ES ES21720109T patent/ES2985378T3/es active Active
- 2021-03-29 JP JP2022559571A patent/JP2023520203A/ja not_active Ceased
- 2021-03-29 KR KR1020227037767A patent/KR20220160661A/ko active Pending
- 2021-03-29 WO PCT/US2021/024695 patent/WO2021202403A1/en not_active Ceased
- 2021-03-29 CA CA3176615A patent/CA3176615A1/en active Pending
- 2021-03-29 IL IL296604A patent/IL296604A/en unknown
- 2021-03-29 EP EP24172605.8A patent/EP4421187A3/en not_active Withdrawn
- 2021-03-29 MX MX2022012184A patent/MX2022012184A/es unknown
- 2021-03-29 BR BR112022019541A patent/BR112022019541A2/pt unknown
- 2021-03-29 AU AU2021249046A patent/AU2021249046A1/en active Pending
- 2021-03-29 US US17/907,166 patent/US20230105642A1/en active Pending
- 2021-03-29 EP EP21720109.4A patent/EP4127220B1/en active Active
- 2021-03-29 CN CN202180034237.XA patent/CN115698319A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| BR112022019541A2 (pt) | 2022-11-16 |
| ES2985378T3 (es) | 2024-11-05 |
| MX2022012184A (es) | 2022-10-27 |
| CA3176615A1 (en) | 2021-10-07 |
| EP4127220A1 (en) | 2023-02-08 |
| EP4421187A3 (en) | 2025-01-08 |
| JP2023520203A (ja) | 2023-05-16 |
| US20230105642A1 (en) | 2023-04-06 |
| AU2021249046A1 (en) | 2022-10-20 |
| EP4127220B1 (en) | 2024-06-05 |
| EP4421187A2 (en) | 2024-08-28 |
| CN115698319A (zh) | 2023-02-03 |
| WO2021202403A1 (en) | 2021-10-07 |
| KR20220160661A (ko) | 2022-12-06 |
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