IL267593B2 - Coagulogen-free amebocyte lysate from Limulus manuliferus - Google Patents

Coagulogen-free amebocyte lysate from Limulus manuliferus

Info

Publication number
IL267593B2
IL267593B2 IL267593A IL26759319A IL267593B2 IL 267593 B2 IL267593 B2 IL 267593B2 IL 267593 A IL267593 A IL 267593A IL 26759319 A IL26759319 A IL 26759319A IL 267593 B2 IL267593 B2 IL 267593B2
Authority
IL
Israel
Prior art keywords
lal
clarified
coagulogen
composition
sample
Prior art date
Application number
IL267593A
Other languages
English (en)
Hebrew (he)
Other versions
IL267593A (en
IL267593B1 (en
Inventor
Candice Stumbaugh
Leben Tadesse
Karen Lin
Original Assignee
Lonza Walkersville Inc
Candice Stumbaugh
Leben Tadesse
Karen Lin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Walkersville Inc, Candice Stumbaugh, Leben Tadesse, Karen Lin filed Critical Lonza Walkersville Inc
Publication of IL267593A publication Critical patent/IL267593A/en
Publication of IL267593B1 publication Critical patent/IL267593B1/en
Publication of IL267593B2 publication Critical patent/IL267593B2/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50Lipopolysaccharides; LPS
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
IL267593A 2017-01-11 2018-01-11 Coagulogen-free amebocyte lysate from Limulus manuliferus IL267593B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762445136P 2017-01-11 2017-01-11
PCT/US2018/013310 WO2018132562A1 (en) 2017-01-11 2018-01-11 Coagulogen-free clarified limulus amebocyte lysate

Publications (3)

Publication Number Publication Date
IL267593A IL267593A (en) 2019-08-29
IL267593B1 IL267593B1 (en) 2024-12-01
IL267593B2 true IL267593B2 (en) 2025-04-01

Family

ID=61249697

Family Applications (1)

Application Number Title Priority Date Filing Date
IL267593A IL267593B2 (en) 2017-01-11 2018-01-11 Coagulogen-free amebocyte lysate from Limulus manuliferus

Country Status (7)

Country Link
US (2) US11352656B2 (enExample)
EP (1) EP3552021B1 (enExample)
JP (2) JP7507560B2 (enExample)
KR (1) KR102545029B1 (enExample)
CN (1) CN110192110B (enExample)
IL (1) IL267593B2 (enExample)
WO (1) WO2018132562A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL264464B2 (en) 2016-08-03 2024-02-01 Lonza Walkersville Inc A method for detecting endotoxin using Limulus extract of amoeba, which is mainly free of coagulogen
US11352656B2 (en) 2017-01-11 2022-06-07 Lonza Walkersville, Inc. Coagulogen-free clarified limulus amebocyte lysate and chromogenic assay of endotoxin
WO2025090132A2 (en) * 2023-05-12 2025-05-01 Life Technolohgies Corporation Methods and kits for detecting endotoxin

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0141685B1 (ko) * 1988-09-01 1998-07-01 야마따니 와따루 참게 아메보사이트 라이세이트 g 인자 활성화 저해제
ATE148230T1 (de) * 1989-10-30 1997-02-15 Biowhittaker Inc Kinetische bestimmungsmethode für endotoxin unter verwendung von limulus-amoebocyten-lysat und chromogenem substrat
JP3122454B2 (ja) * 1990-09-27 2001-01-09 生化学工業株式会社 カブトガニ・アメボサイト・ライセートの調製法
US5208148A (en) 1990-12-07 1993-05-04 Molecular Probes, Inc. Lipophilic fluorescent glycosidase substrates
ATE180018T1 (de) 1991-08-23 1999-05-15 Molecular Probes Inc Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen
US5242805A (en) 1991-08-23 1993-09-07 Molecular Probes, Inc. Long wavelength lipophilic fluorogenic glycosidase substrates
US5576424A (en) 1991-08-23 1996-11-19 Molecular Probes, Inc. Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells
JP3242733B2 (ja) * 1993-02-26 2001-12-25 生化学工業株式会社 エンドトキシン特異的測定剤
US5830912A (en) 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5773236A (en) 1997-04-25 1998-06-30 Molecule Probes, Inc. Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules
US20100203071A1 (en) * 2007-03-21 2010-08-12 Norman Blais Chimeric antigens
CN101855539B (zh) 2007-11-12 2012-08-22 国立大学法人广岛大学 内毒素浓度测定方法及浓度测定用试剂盒
US7846678B2 (en) 2008-08-18 2010-12-07 BioDtech, Inc. Enhancing endotoxin detection
MY155541A (en) 2010-10-29 2015-10-30 Universiti Malaysia Terengganu Process for the preparation of amoebocyte lysate from the haemolymph of the horseshoe crab
CN102305788A (zh) * 2010-11-22 2012-01-04 天津市一瑞生物工程有限公司 革兰阴性菌脂多糖(内毒素)比色检测试剂盒的制备及其使用方法
IL264464B2 (en) 2016-08-03 2024-02-01 Lonza Walkersville Inc A method for detecting endotoxin using Limulus extract of amoeba, which is mainly free of coagulogen
US11352656B2 (en) 2017-01-11 2022-06-07 Lonza Walkersville, Inc. Coagulogen-free clarified limulus amebocyte lysate and chromogenic assay of endotoxin
US20190001330A1 (en) 2017-06-28 2019-01-03 Lonza Walkersville, Inc. Cartridge for endotoxin detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NAKAMURA S ET AL., " FRACTIONATION OF LIMULUS AMEBOCYTE LYSATE - CHARACTERIZATION OF ACTIVATION OF THE PROCLOTTING ENZYME BY AN ENDOTOXIN-MEDIATED ACTIVATOR", 5 October 1982 (1982-10-05) *

Also Published As

Publication number Publication date
US12359241B2 (en) 2025-07-15
JP2020505007A (ja) 2020-02-20
KR20190104387A (ko) 2019-09-09
EP3552021B1 (en) 2022-10-26
KR102545029B1 (ko) 2023-06-19
JP2023002669A (ja) 2023-01-10
IL267593A (en) 2019-08-29
IL267593B1 (en) 2024-12-01
JP7507560B2 (ja) 2024-06-28
CN110192110A (zh) 2019-08-30
JP7724762B2 (ja) 2025-08-18
US11352656B2 (en) 2022-06-07
WO2018132562A1 (en) 2018-07-19
EP3552021A1 (en) 2019-10-16
US20220267824A1 (en) 2022-08-25
CN110192110B (zh) 2023-01-10
US20180208964A1 (en) 2018-07-26

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