IL130886A - Nucleic acid sequencing - Google Patents
Nucleic acid sequencingInfo
- Publication number
- IL130886A IL130886A IL13088698A IL13088698A IL130886A IL 130886 A IL130886 A IL 130886A IL 13088698 A IL13088698 A IL 13088698A IL 13088698 A IL13088698 A IL 13088698A IL 130886 A IL130886 A IL 130886A
- Authority
- IL
- Israel
- Prior art keywords
- label
- dna
- sequence
- stranded
- base sequence
- Prior art date
Links
- 238000012163 sequencing technique Methods 0.000 title claims abstract description 75
- 150000007523 nucleic acids Chemical class 0.000 title description 44
- 102000039446 nucleic acids Human genes 0.000 title description 33
- 108020004707 nucleic acids Proteins 0.000 title description 33
- 239000000523 sample Substances 0.000 claims abstract description 81
- 238000000034 method Methods 0.000 claims abstract description 75
- 108020004414 DNA Proteins 0.000 claims abstract description 71
- 102000003960 Ligases Human genes 0.000 claims abstract description 43
- 108090000364 Ligases Proteins 0.000 claims abstract description 43
- 238000006243 chemical reaction Methods 0.000 claims abstract description 36
- 238000009396 hybridization Methods 0.000 claims abstract description 19
- 102000053602 DNA Human genes 0.000 claims abstract description 16
- 108020004682 Single-Stranded DNA Proteins 0.000 claims abstract description 15
- 230000000295 complement effect Effects 0.000 claims abstract description 11
- 230000003213 activating effect Effects 0.000 claims abstract description 3
- 239000012634 fragment Substances 0.000 claims description 39
- 238000004949 mass spectrometry Methods 0.000 claims description 7
- 238000003491 array Methods 0.000 claims description 4
- 230000000865 phosphorylative effect Effects 0.000 claims description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 40
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 25
- 108091008146 restriction endonucleases Proteins 0.000 description 15
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 230000000903 blocking effect Effects 0.000 description 8
- 238000013459 approach Methods 0.000 description 7
- 238000004422 calculation algorithm Methods 0.000 description 7
- 238000003776 cleavage reaction Methods 0.000 description 7
- 238000002372 labelling Methods 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 230000007017 scission Effects 0.000 description 7
- 238000000329 molecular dynamics simulation Methods 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000007850 fluorescent dye Substances 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 230000027455 binding Effects 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 229960002685 biotin Drugs 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 4
- 230000026731 phosphorylation Effects 0.000 description 4
- 238000006366 phosphorylation reaction Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 238000012804 iterative process Methods 0.000 description 3
- 230000009871 nonspecific binding Effects 0.000 description 3
- 235000021317 phosphate Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- FMKJUUQOYOHLTF-OWOJBTEDSA-N (e)-4-azaniumylbut-2-enoate Chemical compound NC\C=C\C(O)=O FMKJUUQOYOHLTF-OWOJBTEDSA-N 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000011987 methylation Effects 0.000 description 2
- 238000007069 methylation reaction Methods 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 238000002515 oligonucleotide synthesis Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000010647 peptide synthesis reaction Methods 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000010791 quenching Methods 0.000 description 2
- 230000000171 quenching effect Effects 0.000 description 2
- 125000006853 reporter group Chemical group 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 description 1
- 206010011416 Croup infectious Diseases 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000448280 Elates Species 0.000 description 1
- 241001524679 Escherichia virus M13 Species 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 108010064978 Type II Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000012082 adaptor molecule Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol group Chemical group [C@@H]1(CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)[C@H](C)CCCC(C)C HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000027326 copulation Effects 0.000 description 1
- 201000010549 croup Diseases 0.000 description 1
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 125000003976 glyceryl group Chemical group [H]C([*])([H])C(O[H])([H])C(O[H])([H])[H] 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 229920000140 heteropolymer Polymers 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- -1 quaternary ammonium ions Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9700760.3A GB9700760D0 (en) | 1996-10-04 | 1997-01-15 | Nucleic acid sequencing |
| PCT/GB1998/000130 WO1998031831A1 (en) | 1997-01-15 | 1998-01-15 | Nucleic acid sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL130886A0 IL130886A0 (en) | 2001-01-28 |
| IL130886A true IL130886A (en) | 2004-02-19 |
Family
ID=10806027
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL13088698A IL130886A (en) | 1997-01-15 | 1998-01-15 | Nucleic acid sequencing |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7094531B1 (ja) |
| EP (1) | EP0960215A1 (ja) |
| JP (1) | JP2001508308A (ja) |
| AU (1) | AU728805B2 (ja) |
| CA (1) | CA2277520C (ja) |
| IL (1) | IL130886A (ja) |
| NZ (1) | NZ336770A (ja) |
| WO (1) | WO1998031831A1 (ja) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000060124A2 (en) * | 1999-04-06 | 2000-10-12 | Yale University | Fixed address analysis of sequence tags |
| DE19957319A1 (de) * | 1999-11-29 | 2001-05-31 | Febit Ferrarius Biotech Gmbh | Dynamische Bestimmung von Analyten |
| US20020049682A1 (en) * | 2000-09-01 | 2002-04-25 | Nobuko Yamamoto | Authentication certificate, authentication certificate issuance system, and authentication system |
| GB0320059D0 (en) * | 2003-08-27 | 2003-10-01 | Solexa Ltd | A method of sequencing |
| GB0422551D0 (en) * | 2004-10-11 | 2004-11-10 | Univ Liverpool | Labelling and sequencing of nucleic acids |
| EP1844162B1 (en) | 2005-02-01 | 2014-10-15 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
| US20090176675A1 (en) * | 2006-01-09 | 2009-07-09 | Marjorie Mossman Peffly | Personal Care Compositions Containing Cationically Modified Starch and an Anionic Surfactant System |
| US20070160555A1 (en) * | 2006-01-09 | 2007-07-12 | Staudigel James A | Personal care compositions containing cationically modified starch and an anionic surfactant system |
| DE202006020497U1 (de) * | 2006-03-13 | 2008-12-24 | Schubert, Adrian | Vorrichtung zur Erkennung und Identifizierung von Zielstrukturen |
| JP2009538123A (ja) | 2006-04-19 | 2009-11-05 | アプライド バイオシステムズ， エルエルシー | ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー |
| US7811810B2 (en) | 2007-10-25 | 2010-10-12 | Industrial Technology Research Institute | Bioassay system including optical detection apparatuses, and method for detecting biomolecules |
| US7767441B2 (en) * | 2007-10-25 | 2010-08-03 | Industrial Technology Research Institute | Bioassay system including optical detection apparatuses, and method for detecting biomolecules |
| US10174368B2 (en) * | 2009-09-10 | 2019-01-08 | Centrillion Technology Holdings Corporation | Methods and systems for sequencing long nucleic acids |
| US10072287B2 (en) * | 2009-09-10 | 2018-09-11 | Centrillion Technology Holdings Corporation | Methods of targeted sequencing |
| WO2011143556A1 (en) * | 2010-05-13 | 2011-11-17 | Gen9, Inc. | Methods for nucleotide sequencing and high fidelity polynucleotide synthesis |
| EP2405017A1 (en) * | 2010-07-06 | 2012-01-11 | Alacris Theranostics GmbH | Method for nucleic acid sequencing |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0377015B1 (de) * | 1988-05-24 | 1994-11-30 | Gesellschaft für Biotechnologische Forschung mbH (GBF) | Oligonucleotidbank und verfahren zur dna-sequenzierung |
| DE3841565C2 (de) | 1988-12-09 | 1998-07-09 | Europ Lab Molekularbiolog | Verfahren zur Sequenzierung von Nukleinsäuren |
| US5503980A (en) * | 1992-11-06 | 1996-04-02 | Trustees Of Boston University | Positional sequencing by hybridization |
| FR2703693B1 (fr) * | 1993-04-06 | 1995-07-13 | Pasteur Institut | Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic. |
| GB9315847D0 (en) * | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
| WO1995009248A1 (en) | 1993-09-27 | 1995-04-06 | Arch Development Corp. | Methods and compositions for efficient nucleic acid sequencing |
| GB9401200D0 (en) | 1994-01-21 | 1994-03-16 | Medical Res Council | Sequencing of nucleic acids |
| US5750341A (en) * | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
-
1998
- 1998-01-15 US US09/341,641 patent/US7094531B1/en not_active Expired - Fee Related
- 1998-01-15 IL IL13088698A patent/IL130886A/en not_active IP Right Cessation
- 1998-01-15 EP EP98900614A patent/EP0960215A1/en not_active Withdrawn
- 1998-01-15 NZ NZ336770A patent/NZ336770A/xx unknown
- 1998-01-15 AU AU55702/98A patent/AU728805B2/en not_active Ceased
- 1998-01-15 CA CA002277520A patent/CA2277520C/en not_active Expired - Fee Related
- 1998-01-15 WO PCT/GB1998/000130 patent/WO1998031831A1/en not_active Application Discontinuation
- 1998-01-15 JP JP53394498A patent/JP2001508308A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US7094531B1 (en) | 2006-08-22 |
| NZ336770A (en) | 2001-01-26 |
| AU5570298A (en) | 1998-08-07 |
| WO1998031831A1 (en) | 1998-07-23 |
| CA2277520A1 (en) | 1998-07-23 |
| EP0960215A1 (en) | 1999-12-01 |
| JP2001508308A (ja) | 2001-06-26 |
| IL130886A0 (en) | 2001-01-28 |
| CA2277520C (en) | 2006-10-10 |
| AU728805B2 (en) | 2001-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1251136B1 (en) | Method and reagents for analyzing the nucleotide sequence of nucleic acids | |
| AU728805B2 (en) | Nucleic acid sequencing | |
| CA2264581C (en) | Characterising dna | |
| EP1015643B1 (en) | Method and reagents for analyzing the nucleotide sequence of nucleic acids | |
| WO1998015652A1 (en) | Nucleic acid sequencing by adaptator ligation | |
| EP0675966B1 (en) | Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids | |
| US5780231A (en) | DNA extension and analysis with rolling primers | |
| JP4480380B2 (ja) | 分子タグ化システム | |
| US6274373B1 (en) | Gene sequencer and methods | |
| US20050260570A1 (en) | Sequencing by proxy | |
| UA48119C2 (uk) | Спосіб визначення послідовності молекули нуклеїнової кислоти (варіанти) та набір для використання при визначенні послідовності нуклеїнової кислоти | |
| KR20000070230A (ko) | 핵산 종을 감지하고 이를 정량화하는 방법 및 그 조성물 | |
| AU2005225525A1 (en) | Methods and means for nucleic acid sequencing | |
| AU733924B2 (en) | Characterising DNA | |
| JP2022533916A (ja) | 自家発光に基づく単一チャネルシーケンシング法 | |
| JP3419850B2 (ja) | 核酸分別方法 | |
| CA2312945A1 (en) | Method for identifying nucleic acids by electro-spray mass spectrometry | |
| US20220162596A1 (en) | A library of polynucleotides | |
| JPH08238100A (ja) | 所定の配列を有する核酸を特異的に検出する方法、並びに該方法に使用されるプローブ及びキット | |
| US20040248104A1 (en) | Methods and reagents for profiling quantities of nucleic acids | |
| KR20010022917A (ko) | 핵산 종을 감지하고 이를 정량화하는 방법 및 그 조성물 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| MM9K | Patent not in force due to non-payment of renewal fees |