IL100715A - Production of enzymatically active glucocerebrosidase from recombinant cells in production medium suitable for cell viability and secretion and stabilization of said enzyme - Google Patents

Production of enzymatically active glucocerebrosidase from recombinant cells in production medium suitable for cell viability and secretion and stabilization of said enzyme

Info

Publication number
IL100715A
IL100715A IL10071592A IL10071592A IL100715A IL 100715 A IL100715 A IL 100715A IL 10071592 A IL10071592 A IL 10071592A IL 10071592 A IL10071592 A IL 10071592A IL 100715 A IL100715 A IL 100715A
Authority
IL
Israel
Prior art keywords
medium
production
glucocerebrosidase
cell culture
production medium
Prior art date
Application number
IL10071592A
Other languages
English (en)
Other versions
IL100715A0 (en
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Priority to IL157458A priority Critical patent/IL157458A/en
Publication of IL100715A0 publication Critical patent/IL100715A0/xx
Publication of IL100715A publication Critical patent/IL100715A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
IL10071592A 1991-01-21 1992-01-20 Production of enzymatically active glucocerebrosidase from recombinant cells in production medium suitable for cell viability and secretion and stabilization of said enzyme IL100715A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IL157458A IL157458A (en) 1991-01-21 1992-01-20 Composition for stabilizing the secreted glucocerebrosidase from viable, slow growing or non-growing cells in a productive state

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US64415991A 1991-01-21 1991-01-21

Publications (2)

Publication Number Publication Date
IL100715A0 IL100715A0 (en) 1992-09-06
IL100715A true IL100715A (en) 2004-07-25

Family

ID=24583696

Family Applications (2)

Application Number Title Priority Date Filing Date
IL10071592A IL100715A (en) 1991-01-21 1992-01-20 Production of enzymatically active glucocerebrosidase from recombinant cells in production medium suitable for cell viability and secretion and stabilization of said enzyme
IL157458A IL157458A (en) 1991-01-21 1992-01-20 Composition for stabilizing the secreted glucocerebrosidase from viable, slow growing or non-growing cells in a productive state

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL157458A IL157458A (en) 1991-01-21 1992-01-20 Composition for stabilizing the secreted glucocerebrosidase from viable, slow growing or non-growing cells in a productive state

Country Status (5)

Country Link
EP (1) EP0568647B1 (fr)
CA (1) CA2099876C (fr)
DE (2) DE69213714T2 (fr)
IL (2) IL100715A (fr)
WO (1) WO1992013067A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
CA2150956A1 (fr) * 1992-12-10 1994-06-23 Robert G. L. Shorr Conjugues utiles contre les glycolipides et certains polymeres
USH1532H (en) * 1993-11-03 1996-05-07 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US6083725A (en) 1996-09-13 2000-07-04 Transkaryotic Therapies, Inc. Tranfected human cells expressing human α-galactosidase A protein
US7138262B1 (en) 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
JP5570677B2 (ja) 2002-04-25 2014-08-13 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド α−ガラクトシダーゼＡ欠損症の治療
AU2013224728B2 (en) * 2006-02-07 2016-10-20 Takeda Pharmaceutical Company Limited Stabilized Compositions of Proteins Having a Free Thiol Moiety
PL1986612T3 (pl) 2006-02-07 2013-02-28 Shire Human Genetic Therapies Stabilizowana kompozycja glukocerebrozydazy
US8962273B2 (en) 2007-05-11 2015-02-24 Genzyme Corporation Methods of producing a secreted protein
WO2010080720A2 (fr) 2009-01-12 2010-07-15 Nektar Therapeutics Conjugués d'une fraction enzymatique lysosomale et polymère soluble dans l'eau
RU2568831C2 (ru) 2009-07-28 2015-11-20 Шайр Хьюман Дженетик Терапиз Композиции и способы для лечения болезни гоше
WO2013130963A1 (fr) 2012-03-02 2013-09-06 Shire Human Genetic Therapies, Inc. Composition et méthode de traitement de la maladie de gaucher de type iii

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3910822A (en) * 1974-03-14 1975-10-07 Us Health Isolation of glucocerebrosidase from human placental tissue
DE3787805T2 (de) * 1986-08-04 1994-02-10 Garvan Inst Med Res Serumfreies gewebekulturmedium, das ein polymerzellenschutzmittel enthält.
DE68926569T2 (de) * 1988-12-23 1996-09-26 Genzyme Corp Cho-zellen befähigt enzymatisch aktive rekombinante-glukocerebrosidase zu produzieren

Also Published As

Publication number Publication date
IL100715A0 (en) 1992-09-06
CA2099876C (fr) 2002-03-26
EP0568647A1 (fr) 1993-11-10
CA2099876A1 (fr) 1992-07-22
DE122011000008I1 (de) 2012-09-20
IL157458A0 (en) 2004-03-28
DE69213714D1 (de) 1996-10-17
IL157458A (en) 2011-01-31
EP0568647B1 (fr) 1996-09-11
DE69213714T2 (de) 1997-01-23
WO1992013067A1 (fr) 1992-08-06

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