IE37325B1 - Process for monitoring cell and vaccine production - Google Patents
Process for monitoring cell and vaccine productionInfo
- Publication number
- IE37325B1 IE37325B1 IE302/73A IE30273A IE37325B1 IE 37325 B1 IE37325 B1 IE 37325B1 IE 302/73 A IE302/73 A IE 302/73A IE 30273 A IE30273 A IE 30273A IE 37325 B1 IE37325 B1 IE 37325B1
- Authority
- IE
- Ireland
- Prior art keywords
- virus
- level
- assays
- medium
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1414076 Preparaing viral vaccines MERCK & CO INC 1 March 1973 [6 March 1972 14 June 1972] 9978/73 Heading A5B [Also in Division C6] In processes for the controlled manufacture of a virus for human and animal vaccines by replicating virus on sheets of tissue cells in a liquid nutrient medium and harvesting the virus produced, real-time assays are performed to monitor glucose utilisation until the glucose concentration in the medium has decreased to a level in the range 20-60 mg/100 cc, the cell sheet is infected with the desired virus when the foregoing glucose concentration is reached, and then real-time assays are performed to monitor the level of isocitric dehydrogenase in the medium and the virus is harvested when the level of isocitric dehyrogenase has reached the maximum rate of change, whereby the virus is harvested at its maximum titre. According to a modification in processes for the controlled manufacture of tissue cells real-time assays are performed to monitor glucose utilization until the glucose concentration in the medium has decreased to a level in the range 5-15 mg/100 cc and the cells are then harvested, whereby the cell harvest is maximised. Examples 1 to 4 are directed to the propugation of mumps, measles or rubella virus in chick or duck embryo cells. In certain of these the use of glutamic-oxalacetic transaminase assays is disclosed in connection with achieving maximum titre of virus (c.f. the isocitric dehydrogenase assays given above). Examples 5 to 7 are directed to the culturing of WI-33, chick embryo and duck embryo cells, respectively.
[GB1414076A]
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US23221172A | 1972-03-06 | 1972-03-06 | |
US26282972A | 1972-06-14 | 1972-06-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
IE37325L IE37325L (en) | 1973-09-06 |
IE37325B1 true IE37325B1 (en) | 1977-06-22 |
Family
ID=26925770
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE302/73A IE37325B1 (en) | 1972-03-06 | 1973-02-27 | Process for monitoring cell and vaccine production |
Country Status (4)
Country | Link |
---|---|
JP (1) | JPS5915887B2 (en) |
GB (1) | GB1414076A (en) |
IE (1) | IE37325B1 (en) |
NL (1) | NL7302283A (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5278048A (en) * | 1988-10-21 | 1994-01-11 | Molecular Devices Corporation | Methods for detecting the effect of cell affecting agents on living cells |
EP0394406B1 (en) * | 1988-10-21 | 1995-04-26 | Molecular Devices Corporation | Methods and apparatus for detecting the effect of cell affecting agents on living cells |
-
1973
- 1973-02-19 NL NL7302283A patent/NL7302283A/xx not_active Application Discontinuation
- 1973-02-27 IE IE302/73A patent/IE37325B1/en unknown
- 1973-03-01 GB GB997873A patent/GB1414076A/en not_active Expired
- 1973-03-06 JP JP48025770A patent/JPS5915887B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
GB1414076A (en) | 1975-11-19 |
IE37325L (en) | 1973-09-06 |
JPS5915887B2 (en) | 1984-04-12 |
JPS48103719A (en) | 1973-12-26 |
NL7302283A (en) | 1973-09-10 |
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