GB1412244A - Enzyme preparations and microbiological production thereof - Google Patents

Enzyme preparations and microbiological production thereof

Info

Publication number
GB1412244A
GB1412244A GB4409671A GB4409671A GB1412244A GB 1412244 A GB1412244 A GB 1412244A GB 4409671 A GB4409671 A GB 4409671A GB 4409671 A GB4409671 A GB 4409671A GB 1412244 A GB1412244 A GB 1412244A
Authority
GB
United Kingdom
Prior art keywords
cholesterol
sept
micro
organism
erythropolis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4409671A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Priority to GB4409671A priority Critical patent/GB1412244A/en
Publication of GB1412244A publication Critical patent/GB1412244A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Abstract

1412244 Cholesterol dehydrogenase ex Nocar. dia erythropolis BOEHRINGER MANNHEIM GmbH 21 Sept 1972 [22 Sept 1971] 44096/71 Heading C3H [Also in Division G1] A cell free enzyme preparation, free from cholesterol and having cholesterol dehydrogenase activity is obtained by cultivating the micro-organism Nocardia erythropolis in presence of cholesterol as sole carbon source. The cultivation is preferably carried out by inoculating the micro-organism into a salt-cholesterol medium, culturing, harvesting by centrifugation and then suspending the cells as a paste in a phosphate buffer and disrupting them by sonication, deep freezing or expressing them through a small hole. The enzyme is used to assay cholesterol in serum by virtue of its conversion to cholestenone (estimated) quantitatively by U.V. spectroscopy) (see Division G1).
GB4409671A 1971-09-22 1971-09-22 Enzyme preparations and microbiological production thereof Expired GB1412244A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB4409671A GB1412244A (en) 1971-09-22 1971-09-22 Enzyme preparations and microbiological production thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4409671A GB1412244A (en) 1971-09-22 1971-09-22 Enzyme preparations and microbiological production thereof

Publications (1)

Publication Number Publication Date
GB1412244A true GB1412244A (en) 1975-10-29

Family

ID=10431761

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4409671A Expired GB1412244A (en) 1971-09-22 1971-09-22 Enzyme preparations and microbiological production thereof

Country Status (1)

Country Link
GB (1) GB1412244A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4892816A (en) * 1984-10-31 1990-01-09 Amano Pharmaceutical Co., Ltd. Method for the determination of cholesterol
EP0709456A2 (en) * 1994-10-26 1996-05-01 Roche Diagnostics GmbH Microbial cholesterol-dehydrogenase, process for the preparation and use
WO1998020150A1 (en) * 1996-11-07 1998-05-14 Kyowa Hakko Kogyo Co., Ltd. Process for preparing cholesterol-lowering compound
US5856156A (en) * 1994-10-26 1999-01-05 Boehringer Mannheim Gmbh Microbial cholesterol dehydrogenase, process for its production and use

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4892816A (en) * 1984-10-31 1990-01-09 Amano Pharmaceutical Co., Ltd. Method for the determination of cholesterol
EP0709456A2 (en) * 1994-10-26 1996-05-01 Roche Diagnostics GmbH Microbial cholesterol-dehydrogenase, process for the preparation and use
US5856156A (en) * 1994-10-26 1999-01-05 Boehringer Mannheim Gmbh Microbial cholesterol dehydrogenase, process for its production and use
EP0709456A3 (en) * 1994-10-26 1999-08-11 Roche Diagnostics GmbH Microbial cholesterol-dehydrogenase, process for the preparation and use
WO1998020150A1 (en) * 1996-11-07 1998-05-14 Kyowa Hakko Kogyo Co., Ltd. Process for preparing cholesterol-lowering compound
US6485931B2 (en) 1996-11-07 2002-11-26 Kyowa Hakko Kogyo Co., Ltd. Cholesterol dehydrogenase, coprostan-3-one dehydrogenase and 4-cholesten-3-one dehydrogenase, compositions containing the dehydrogenases, and method for reducing amount of cholesterol using the compositions

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee