SU611597A3 - Method of cultivating streptococcus hemolyticus - Google Patents

Method of cultivating streptococcus hemolyticus

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Publication number
SU611597A3
SU611597A3 SU671192686A SU1192686A SU611597A3 SU 611597 A3 SU611597 A3 SU 611597A3 SU 671192686 A SU671192686 A SU 671192686A SU 1192686 A SU1192686 A SU 1192686A SU 611597 A3 SU611597 A3 SU 611597A3
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SU
USSR - Soviet Union
Prior art keywords
streptococcus hemolyticus
medium
cultivating
cultivating streptococcus
extract
Prior art date
Application number
SU671192686A
Other languages
Russian (ru)
Inventor
Огава Харуки
Сузуки Сигео
Савада Микио
Ямамото Акихиро
Original Assignee
Чугаи Сейяку Кабусики Каиша И Кийова Хакко Когио Ко,Лтд (Фирма)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Чугаи Сейяку Кабусики Каиша И Кийова Хакко Когио Ко,Лтд (Фирма) filed Critical Чугаи Сейяку Кабусики Каиша И Кийова Хакко Когио Ко,Лтд (Фирма)
Application granted granted Critical
Publication of SU611597A3 publication Critical patent/SU611597A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

(54) СПОСОБ КУЛЬТИВИРОВАНИЯ STREPTOCOCCUS HEMOLYTiCUS(54) METHOD FOR CULTIVATING STREPTOCOCCUS HEMOLYTiCUS

Изобретение относитс  к микробиологии и касаетс  получени  клеток гемолитического стрептококка с высокой противоопухолевой активностьюThis invention relates to microbiology and relates to the production of hemolytic streptococcus cells with high antitumor activity.

Известен способ культивировани  Streptococcus hemoryticus на питательной среде, содержащей экстракт бычьего сердца, пептон, хлористый натрий, нукленнат натри , фосфат натри , бикарбонат натри , ацид натри , хлористый литий рибофлавин , уксуснокислый таллий, дефибршшрованную кровь и мальтозу с последующим отделением клеток от среды 1. Однако известный способ ие обеспечивает высокой противоопухолевой активности культуры.A method is known culturing Streptococcus hemoryticus on a nutrient medium containing the extract of bovine heart, peptone, sodium chloride, nuklennat, sodium phosphate, sodium bicarbonate, Acid sodium chloride, lithium riboflavin acetate thallium defibrshshrovannuyu blood and maltose, followed by separating the cells from the medium 1. However, the known method does not provide a high antitumor activity of the culture.

Целью изобретени   вл етс  повышение противоопухолевой активности культуры.The aim of the invention is to increase the antitumor activity of the culture.

Эта цель достигаетс  тем, что штамм Streptococcus hemolyticus АТСС 21060 выращивают на среде, содержащей 3,0-6,0% дрожжевого экстракта , при рН 7,0-7,4.This goal is achieved by the fact that the strain Streptococcus hemolyticus ATCC 21060 is grown on medium containing 3.0-6.0% yeast extract, at pH 7.0-7.4.

Среду, которую используют дл  выращивани  культуры, получают путем растворени , например экстракта дрожжей или автолизата дрожжей в воде, нагревани  раствора после нейтрализации и последующей фильтрации дл  отделени  нерастворимой фракции, рНГ среды 7,0-7,4, концентраци  дрожжевого экстракта 3,0-6,0%. Могут быть использованы также пептоны; или экстракты (рыбные или м сные из гов дины, конского или китового м са). В качестве неоргаиических солей можно использовать , например хлористый натрий, первичный фосфат натри  или натри . Хорошие результаты получают при добавке рибонуклеиновой кислоты шш рибонуклеазы. Посев бактерий производ т путем добавки к среде бактериальных клеток, подвергнутых предварительному выращиванию при температуре 30-40 С, преимущественно при 37°С. В качестве среды дл  предварительного выращивани  используют дрожжевой экстракт. В том случае , когда дл  получени  культуры используют щ ожжевой зкстракт, противоопухолева  активность бактериальных клеток, котора  увеличиваетс  во врем  выращивани , несколько снижаетс  по мере удлинени  этого времени. Если дл  предварительного выращивани  используют м сную воду, то допустимое врем  дл  выращивани  бактерий увеличиваетс , лучще использовать дл  этого температуру 37° С и врем  12-30 ч.The medium used to grow the culture is obtained by dissolving, for example, a yeast extract or yeast autolysate in water, heating the solution after neutralization and subsequent filtration to separate the insoluble fraction, the pH of the medium 7.0-7.4, the concentration of the yeast extract 3.0- 6.0%. Peptones can also be used; or extracts (fish or meat from beef, horse or whale meat). As inorganic salts, for example, sodium chloride, primary sodium or sodium phosphate can be used. Good results are obtained with the addition of ribonucleic acid shsh ribonuclease. Sowing of bacteria is performed by adding to the medium bacterial cells subjected to preliminary cultivation at a temperature of 30-40 ° C, mainly at 37 ° C. Yeast extract is used as a pre-growth medium. In the case where the Shcheln extract is used to obtain the culture, the antitumor activity of the bacterial cells, which increases during the growth, decreases slightly as this time lengthens. If meat is used for pre-cultivation, the permissible time for bacterial growth is increased, it is better to use a temperature of 37 ° C and a time of 12-30 hours for this.

Можно использовать и другие среды, например состо цдае из м сного экстракта, пептона и хлористого натри . Пбсле завершени  выращивани Other media can be used, for example, consisting of meat extract, peptone and sodium chloride. Completion of cultivation

SU671192686A 1966-10-31 1967-10-25 Method of cultivating streptococcus hemolyticus SU611597A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7134766 1966-10-31

Publications (1)

Publication Number Publication Date
SU611597A3 true SU611597A3 (en) 1978-06-15

Family

ID=13457858

Family Applications (1)

Application Number Title Priority Date Filing Date
SU671192686A SU611597A3 (en) 1966-10-31 1967-10-25 Method of cultivating streptococcus hemolyticus

Country Status (7)

Country Link
CH (1) CH486884A (en)
DK (1) DK115653B (en)
FR (1) FR1551107A (en)
GB (1) GB1157947A (en)
NL (1) NL142991B (en)
SE (1) SE349324B (en)
SU (1) SU611597A3 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2079785B (en) 1980-04-14 1984-10-03 Kanebo Ltd Process for cultivation of hemolytic streptococcus pyogenes

Also Published As

Publication number Publication date
DK115653B (en) 1969-10-27
SE349324B (en) 1972-09-25
GB1157947A (en) 1969-07-09
DE1617416A1 (en) 1972-02-17
FR1551107A (en) 1968-12-27
NL142991B (en) 1974-08-15
NL6714739A (en) 1968-05-01
CH486884A (en) 1970-03-15

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