GB1157947A - Process for obtaining Cells of Hemolytic Streptococci having High Anti-Tumor Activity - Google Patents

Process for obtaining Cells of Hemolytic Streptococci having High Anti-Tumor Activity

Info

Publication number
GB1157947A
GB1157947A GB49275/67A GB4927567A GB1157947A GB 1157947 A GB1157947 A GB 1157947A GB 49275/67 A GB49275/67 A GB 49275/67A GB 4927567 A GB4927567 A GB 4927567A GB 1157947 A GB1157947 A GB 1157947A
Authority
GB
United Kingdom
Prior art keywords
medium
extract
hemolytic streptococci
yeast extract
decilitre
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB49275/67A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chugai Pharmaceutical Co Ltd
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of GB1157947A publication Critical patent/GB1157947A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Abstract

1,157,947. Streptococcus hemolyticus of high anti-tumour activity. CHUGAI SEIYAKU K.K. Oct. 30, 1967 [Oct. 31, 1966], No.49275/67. Heading C6F. A process for obtaining cells of hemolytic Streptococci having high anti-tumour activity involves cultivating hemolytic Streptococci having anti-tumour activity, particularly Streptococcus hemolyticus ATCC No. 21060, in a culture medium containing a yeast extract, and separating bacterial cells from the cultivated broth. The yeast extracted may be obtained by dissolving a commerically available yeast extract or auto-lysate in water, neutralizing and heating the solution, and then taking away the insoluble fraction by filtering. The culture medium of pH 7À0-7À4 typically contains between 3 and 6 grams per decilitre of yeast extract and such other constituents as peptone, fish meat extract, beef extract, horse or whale flesh extract, ribonucleic acid and/or its ribonuclease core, sodium chloride, sodium hydrogen phosphate and sodium hydrogen carbonate. The hemolytic Streptococci may be preliminarily cultivated in a yeast extract medium or beef infusion medium at 37‹ C. for 24 hours. The main cultivation is then carried out at 37‹ C. for periods ranging from 10 to 30 hours (according to circumstances). The separated cells are suspended in a medium containing penicillin and heated at 30-38‹ C. for 10-30 minutes and then at 40-50‹ C. for 20-40 minutes to eliminate their virulence and hemolysin-producing activity. A suitable suspension medium (Bernheimer's Basal Medium) is composed of (a) 6 ml. of a solution consisting of 675 mg. of maltose and an aqueous solution containing 20 grams 1 decilitre of potassium dihydrogen phosphate, adjusted to pH 6À9-7À0 with sodium hydroxide, (b) 12 ml. of an aqueous solution containing 2 grams 1 decilitre of magnesium sulphate heptahydrate and (c) 66 ml. of distilled water.
GB49275/67A 1966-10-31 1967-10-30 Process for obtaining Cells of Hemolytic Streptococci having High Anti-Tumor Activity Expired GB1157947A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7134766 1966-10-31

Publications (1)

Publication Number Publication Date
GB1157947A true GB1157947A (en) 1969-07-09

Family

ID=13457858

Family Applications (1)

Application Number Title Priority Date Filing Date
GB49275/67A Expired GB1157947A (en) 1966-10-31 1967-10-30 Process for obtaining Cells of Hemolytic Streptococci having High Anti-Tumor Activity

Country Status (7)

Country Link
CH (1) CH486884A (en)
DK (1) DK115653B (en)
FR (1) FR1551107A (en)
GB (1) GB1157947A (en)
NL (1) NL142991B (en)
SE (1) SE349324B (en)
SU (1) SU611597A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4306024A (en) 1980-04-14 1981-12-15 Kanebo Ltd. Process for cultivation of hemolytic Streptococcus pyogenes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4306024A (en) 1980-04-14 1981-12-15 Kanebo Ltd. Process for cultivation of hemolytic Streptococcus pyogenes

Also Published As

Publication number Publication date
CH486884A (en) 1970-03-15
FR1551107A (en) 1968-12-27
SE349324B (en) 1972-09-25
DK115653B (en) 1969-10-27
SU611597A3 (en) 1978-06-15
DE1617416A1 (en) 1972-02-17
NL142991B (en) 1974-08-15
NL6714739A (en) 1968-05-01

Similar Documents

Publication Publication Date Title
US3891504A (en) Process for the manufacture of zeaxanthin
Gunz Reduction of tetrazolium salts by some biological agents
Barnett Nutritional requirements for axenic growth of some haustorial mycoparasites
US3627644A (en) Process for the cultivation of hemolytic streptococci
DE1617397C3 (en) Production of a tumor static agent by growing Streptococcus haemolyticus
GB1157947A (en) Process for obtaining Cells of Hemolytic Streptococci having High Anti-Tumor Activity
Enebo Symbiosis in thermophilic cellulose fermentation
SU575038A3 (en) Method of preparing antibiotic
DE2512606A1 (en) PROCESS FOR PRODUCING A CHOLESTEROL OXIDASE ENZYME
US3674866A (en) Moenomycin and process for producing same
US4306024A (en) Process for cultivation of hemolytic Streptococcus pyogenes
US4477437A (en) Substances having carcinostatic and immunostimulating activity
US3814795A (en) Antibiotic s 15-1 and process for producing the same
US3907989A (en) Novel antibiotic No. 1998 and process for producing the same
US4591558A (en) Novel substances having antitumor and immunostimulating activity, process for preparing the same and antitumor agent containing the same
DE1792038C3 (en) Process for the preparation of a mixture of approximately equal parts ergotamine and ergocryptine
Baijal A physiological study of Saksenaea vasiformis Saksena
GB593953A (en) Improvements in or relating to the production of riboflavin in an aqueous medium
Irani et al. Occurrence of sialic acid in some gram-positive and gram-negative pathogenic bacteria
Braude et al. Effect of Double stimulation on macrophage function
Gustafson et al. Chemical characteristics of Leptotrichia buccalis endotoxin
US3122574A (en) Variotin and its production and stabilization
US2922746A (en) Microbiological product and method of making the same
GB1134678A (en) Process for promoting bacterial sporulation
US3699002A (en) Dextran hydrolizing enzymes

Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years