SU1193168A1 - Method of obtaining man's epiderma cell culture - Google Patents

Method of obtaining man's epiderma cell culture Download PDF

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Publication number
SU1193168A1
SU1193168A1 SU833659090A SU3659090A SU1193168A1 SU 1193168 A1 SU1193168 A1 SU 1193168A1 SU 833659090 A SU833659090 A SU 833659090A SU 3659090 A SU3659090 A SU 3659090A SU 1193168 A1 SU1193168 A1 SU 1193168A1
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SU
USSR - Soviet Union
Prior art keywords
human
units
substrate
skin
cell culture
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SU833659090A
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Russian (ru)
Inventor
Борис Карпович Гаврилюк
Борис Борисович Егоров
Михаил Ильич Кузин
Генрих Романович Иваницкий
Владимир Константинович Сологуб
Сергей Сергеевич Морозов
Владимир Георгиевич Ивков
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Институт биологической физики АН СССР
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Priority to SU833659090A priority Critical patent/SU1193168A1/en
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Publication of SU1193168A1 publication Critical patent/SU1193168A1/en

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Abstract

СПОСОБ ПОЛУЧЕНИЯ КУЛЬТУРЫ КЛЕТОК ЭПИДЕРМИСА ЧЕЛОВЕКА, включающий получение из лоскутов кожи исходной суспензии клеток с последующим культивированием ее на подложке в питательной среде 199 или в среде Игла, содержащей витамины и гидрокрртизон , отличающийс  тем, что, с целью ускорени  роста культуры путем повышени  ее физиологической и пролиферативной активности, исходную суспензию клеток получают из регенерирующих участков кожи или лоскутов кожи, предварительно вьщержанных на раневой ожоговой поверхности в течение 1-2 сут или в среде Игла, содержащей 10-20% змбриональной сыворотки человека, 0,1-0,2ед/мл инсулина и 0,1-0,2 ед/мл соматотропного гормона, и культивируют на подложке из человеческого коллагена в питательной среде, дополнительно содержащей 10-20% плацентарной сыворотки и 1-2% плацентарного экстракта.A METHOD FOR OBTAINING A HUMAN EPIDERMIS CELL CULTURE, including obtaining an initial cell suspension from skin grafts and then cultivating it on a substrate in a nutrient medium 199 or in the Eagle's medium containing vitamins and hydrophysylase, which is designed to increase its physiological activity proliferative activity, the initial cell suspension is obtained from regenerating areas of skin or skin grafts, previously held on the wound burn surface for 1-2 days or in Needle food containing 10–20% human zmbryonic serum, 0.1–0.2 units / ml of insulin and 0.1–0.2 units / ml of somatotropic hormone, and cultured on a substrate of human collagen in a nutrient medium, additionally containing 10 -20% placental serum and 1-2% placental extract.

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Claims (1)

СПОСОБ ПОЛУЧЕНИЯ КУЛЬТУРЫ КЛЕТОК ЭПИДЕРМИСА ЧЕЛОВЕКА, включающий получение из лоскутов кожи исходной суспензии клеток с последующим культивированием ее на подложке в питательной среде 199 или в среде METHOD FOR PREPARING A HUMAN EPIDERMIS CELL CULTURE, comprising obtaining from the skin flaps an initial cell suspension with subsequent cultivation on a substrate in nutrient medium 199 or in medium Игла, содержащей витамины и гидрокрртизон, отличающийся тем, что, с целью ускорения роста культуры путем повышения ее физиологической и пролиферативной активности, исходную суспензию клеток получают из регенерирующих участков кожи или лоскутов кожи, предварительно выдер жанных на раневой ожоговой поверхности в течение 1-2 сут или в среде Игла, содержащей 10-20% эмбриональной сыворотки человека, 0,1-0,2ед/мл инсулина и 0,1-0,2 ед/мл соматотропного гормона, и культивируют на подложке из человеческого коллагена в питательной среде, дополнительно содержащей 10-20% плацентарной сыворотки и 1-2% плацентарного экстракта.A needle containing vitamins and hydrocortisone, characterized in that, in order to accelerate the growth of the culture by increasing its physiological and proliferative activity, the initial suspension of cells is obtained from regenerating areas of the skin or skin flaps that have previously been kept on the wound burn surface for 1-2 days or in Needle medium containing 10-20% of human fetal serum, 0.1-0.2 units / ml insulin and 0.1-0.2 units / ml growth hormone, and cultured on a substrate of human collagen in a nutrient medium, additionally with ERZHAN placental 10-20% whey and 1-2% of placental extract. >> 1 1193168 21 1193168 2
SU833659090A 1983-11-03 1983-11-03 Method of obtaining man's epiderma cell culture SU1193168A1 (en)

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SU833659090A SU1193168A1 (en) 1983-11-03 1983-11-03 Method of obtaining man's epiderma cell culture

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SU833659090A SU1193168A1 (en) 1983-11-03 1983-11-03 Method of obtaining man's epiderma cell culture

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SU1193168A1 true SU1193168A1 (en) 1985-11-23

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SU833659090A SU1193168A1 (en) 1983-11-03 1983-11-03 Method of obtaining man's epiderma cell culture

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1063226C (en) * 1997-12-02 2001-03-14 中国科学院新疆化学研究所 Detecting and screening method for biological active additive component of cosmetics

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Авторское свидетельство СССР № 1097671, кл. С 12 N 5/00, 1983. ( *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1063226C (en) * 1997-12-02 2001-03-14 中国科学院新疆化学研究所 Detecting and screening method for biological active additive component of cosmetics

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