HUT51672A - Process for producing recombinant expression dna vectors and dna molecules encoding deacetoxycefalosporin c synthetase enzyme - Google Patents

Process for producing recombinant expression dna vectors and dna molecules encoding deacetoxycefalosporin c synthetase enzyme

Info

Publication number
HUT51672A
HUT51672A HU892208A HU220889A HUT51672A HU T51672 A HUT51672 A HU T51672A HU 892208 A HU892208 A HU 892208A HU 220889 A HU220889 A HU 220889A HU T51672 A HUT51672 A HU T51672A
Authority
HU
Hungary
Prior art keywords
deacetoxycefalosporin
recombinant expression
molecules encoding
producing recombinant
synthetase enzyme
Prior art date
Application number
HU892208A
Other languages
English (en)
Other versions
HU209581B (en
Inventor
Thomas D Ingolia
James R Miller
Paul L Skatrud
Steven Kovacevic
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of HUT51672A publication Critical patent/HUT51672A/hu
Publication of HU209581B publication Critical patent/HU209581B/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
HU892208A 1988-05-09 1989-05-08 Process for high-level expression of desacetoxycephalosporin c synthetase ensime in host cell containing recombinant dna HU209581B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/192,273 US5070020A (en) 1988-05-09 1988-05-09 Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase

Publications (2)

Publication Number Publication Date
HUT51672A true HUT51672A (en) 1990-05-28
HU209581B HU209581B (en) 1994-08-29

Family

ID=22708994

Family Applications (1)

Application Number Title Priority Date Filing Date
HU892208A HU209581B (en) 1988-05-09 1989-05-08 Process for high-level expression of desacetoxycephalosporin c synthetase ensime in host cell containing recombinant dna

Country Status (7)

Country Link
US (1) US5070020A (hu)
EP (1) EP0341892A1 (hu)
JP (1) JPH0227984A (hu)
AU (1) AU622662B2 (hu)
DK (1) DK217889A (hu)
HU (1) HU209581B (hu)
IL (1) IL90129A0 (hu)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
US6709859B1 (en) * 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
CA2045951A1 (en) * 1990-07-06 1992-01-07 Steven Kovacevic Recombinant dna expression vectors and dna compounds that encode deacetylcephalosporin c synthase activity
US5318896A (en) * 1991-09-11 1994-06-07 Merck & Co., Inc. Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
HU219370B (en) * 1991-10-15 2001-03-28 Gist Brocades Bv Novel bioprocesses for preparing 7-aca and 7-adac
US5554507A (en) * 1992-07-30 1996-09-10 American Cyanamid Company Bacillus subtilis siderophore genes
HU219265B (en) * 1993-07-30 2001-03-28 Gist Brocades Bv Process for the efficient production of 7-adca via 3-(carboxyethylthio)propionyl-7-adca, recombinant dna vectors and host cells
JPH09512170A (ja) * 1994-04-22 1997-12-09 メルク エンド カンパニー インコーポレーテッド セファマイシン生合成後期酵素コーディングｄｎａ
WO1996000787A1 (en) * 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
CN1084791C (zh) * 1995-06-02 2002-05-15 吉斯特·布罗卡迪斯股份有限公司 通过扩环酶活性作用于青霉素g生产7－adca的方法
US5919680A (en) * 1995-11-27 1999-07-06 Isis Innovation Limited Process for the production of SSC's via expandase activity on penicillin G
AU4114697A (en) * 1996-07-16 1998-02-09 Gist-Brocades B.V. Improved process for the production of adipoyl cephalosporins
US6071724A (en) * 1996-12-13 2000-06-06 Eli Lilly And Company Streptococcus pneumoniaegene sequence era
BR9910532A (pt) 1998-05-19 2001-10-16 Dsm Nv Produção melhorada in vivo de cefalosporina
US6720479B1 (en) * 1998-05-29 2004-04-13 Iowa State University Research Foundation, Inc. Plant retroelements and methods related thereto
US7094953B2 (en) 1998-05-29 2006-08-22 Iowa State University Research Foundation, Inc. Plant retroelements and methods related thereto
US6284483B1 (en) * 1999-10-06 2001-09-04 Board Of Trustees Operating Michigan State University Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate
CA2451654A1 (en) * 2001-06-22 2003-01-03 Ceres, Inc. Chimeric histone acetyltransferase polypeptides
KR101021695B1 (ko) 2001-07-12 2011-03-15 유니버시티 오브 매사추세츠 유전자 불활성화를 매개하는 소형 간섭 ｒｎａ의 생체내 제조
EP1900815B1 (en) 2001-07-12 2016-09-07 University of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US6699699B2 (en) 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
ES2400562T3 (es) 2003-05-28 2013-04-10 Dsm Sinochem Pharmaceuticals Netherlands B.V. Compuesto de cefem
ES2357116T5 (es) 2003-06-02 2021-09-03 Univ Massachusetts Métodos y composiciones para mejorar la eficacia y especificad de FNAi
ES2712695T3 (es) 2003-06-02 2019-05-14 Univ Massachusetts Métodos y composiciones para controlar la eficacia de la silenciación del ARN
WO2007107810A1 (en) * 2006-03-20 2007-09-27 Council Of Scientific And Industrial Research A method for detecting the presence of water born pathogens and indicator microorganism
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
CN105132387A (zh) * 2008-12-22 2015-12-09 绿光生物科学公司 用于制造化合物的组合物和方法
KR20130071433A (ko) 2010-05-07 2013-06-28 그린라이트 바이오사이언시스, 아이엔씨. 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법
US8916358B2 (en) 2010-08-31 2014-12-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
JP2014526250A (ja) 2011-09-09 2014-10-06 グリーンライト バイオサイエンシーズ インコーポレーテッド カルバペネムの無細胞調製
AU2014306074B2 (en) 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
KR20230048162A (ko) 2015-03-30 2023-04-10 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
WO2017176963A1 (en) 2016-04-06 2017-10-12 Greenlight Biosciences, Inc. Cell-free production of ribonucleic acid
MX2020003841A (es) 2017-10-11 2020-11-06 Greenlight Biosciences Inc Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510246A (en) * 1982-08-23 1985-04-09 Queen's University At Kingston Isolation of cyclase, epimerase and a ring expansion enzyme for producing unnatural cephalosporins
US4536476A (en) * 1982-08-23 1985-08-20 Queen's University At Kingston Stable epimerase reagent, cyclase reagent and ring expansion reagent for cell-free production of cephalosporins
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.

Also Published As

Publication number Publication date
HU209581B (en) 1994-08-29
DK217889A (da) 1989-11-13
JPH0227984A (ja) 1990-01-30
AU622662B2 (en) 1992-04-16
IL90129A0 (en) 1989-12-15
US5070020A (en) 1991-12-03
DK217889D0 (da) 1989-05-03
EP0341892A1 (en) 1989-11-15
AU3408289A (en) 1989-11-09

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Legal Events

Date Code Title Description
HMM4 Cancellation of final prot. due to non-payment of fee