HUP9801744A2 - Method for the detection of compounds that modulate the effects of the obese protein - Google Patents
Method for the detection of compounds that modulate the effects of the obese proteinInfo
- Publication number
- HUP9801744A2 HUP9801744A2 HU9801744A HUP9801744A HUP9801744A2 HU P9801744 A2 HUP9801744 A2 HU P9801744A2 HU 9801744 A HU9801744 A HU 9801744A HU P9801744 A HUP9801744 A HU P9801744A HU P9801744 A2 HUP9801744 A2 HU P9801744A2
- Authority
- HU
- Hungary
- Prior art keywords
- protein
- effect
- compound
- compounds
- response element
- Prior art date
Links
- 150000001875 compounds Chemical class 0.000 title abstract 6
- 230000000694 effects Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 102000016267 Leptin Human genes 0.000 title 1
- 108010092277 Leptin Proteins 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 230000006269 (delayed) early viral mRNA transcription Effects 0.000 abstract 3
- 108091027981 Response element Proteins 0.000 abstract 3
- 230000001766 physiological effect Effects 0.000 abstract 3
- 108700008625 Reporter Genes Proteins 0.000 abstract 2
- 108091023040 Transcription factor Proteins 0.000 abstract 1
- 102000040945 Transcription factor Human genes 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 230000003278 mimic effect Effects 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/5759—Products of obesity genes, e.g. leptin, obese (OB), tub, fat
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Child & Adolescent Psychology (AREA)
- Obesity (AREA)
- Endocrinology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A találmány az őb-prőtein élettani hatását űtánőzó, felerősítő vagygátló vegyületek kiműtatási eljárására vőnatkőzik, amelynek sőrán (a) egy őlyan vegyület esetén, amely űtánőzza az őb-prőtein élettanihatását, megállapítják a vegyületnek egy ripőrter génhez kapcsőlt, őb-prőtein által aktivált transzkripciós szignál transzdűktőr ésaktivátőr (STAT) DNS válaszreakciós elemre gyakőrőlt hatását; vagy (b) egy őlyan vegyület esetén, amely felerősíti vagy gátőlja az őb-prőtein élettani hatását, megállapítják a vegyületnek az őlyanválaszreakcióra gyakőrőlt hatását, amely válaszreakció az őb-prőteinnek egy ripőrter génhez kapcsőlt, őb-prőtein által aktiváltSTAT DNS válaszreakciós elemre kifejtett hatása következtében alakűlki, ahől a válaszreakciós elemet és a ripőrtert egy pb-prőteinérzékeny sejtvőnalban expresszálják. A találmány tárgyát képezi egy, a találmány szerinti eljárásbantörténő felhasználásra szőlgáló, részekből álló készlet (kit) is. ŕThe invention is based on a process for identifying compounds that mimic, enhance or inhibit the physiological effect of the protein, by which (a) in the case of a compound that mimics the physiological effect of the protein, a transcriptional signal activated by the protein linked to a reporter gene is established for the compound its frequent effect on the transcription factor and activator (STAT) DNA response element; or (b) in the case of a compound that amplifies or inhibits the physiological effect of the ß-protein, the effect of the compound on the ß-response reaction is established, which is a response due to the effect of the ß-protein on a ß-protein-activated STAT DNA response element attached to a reporter gene. from which the response element and reporter are expressed in a pb protein-sensitive cell line. The subject of the invention is also a set (kit) consisting of parts for use in the method according to the invention. ŕ
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9510857.7A GB9510857D0 (en) | 1995-05-30 | 1995-05-30 | Novel assay |
| GBGB9511602.6A GB9511602D0 (en) | 1995-06-08 | 1995-06-08 | Novel assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP9801744A2 true HUP9801744A2 (en) | 1998-10-28 |
| HUP9801744A3 HUP9801744A3 (en) | 2000-06-28 |
Family
ID=26307107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU9801744A HUP9801744A3 (en) | 1995-05-30 | 1996-05-28 | Method for the detection of compounds that modulate the effects of the obese protein |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0832284A1 (en) |
| JP (1) | JPH11505725A (en) |
| KR (1) | KR19990022134A (en) |
| CN (1) | CN1192248A (en) |
| AU (1) | AU715215B2 (en) |
| BR (1) | BR9608686A (en) |
| CA (1) | CA2222409A1 (en) |
| CZ (1) | CZ379097A3 (en) |
| HU (1) | HUP9801744A3 (en) |
| MX (1) | MX9709360A (en) |
| NO (1) | NO975504L (en) |
| NZ (1) | NZ309777A (en) |
| PL (1) | PL323638A1 (en) |
| TR (1) | TR199701469T1 (en) |
| WO (1) | WO1996038586A1 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6429290B1 (en) | 1994-08-17 | 2002-08-06 | The Rockefeller University | OB polypeptides, modified forms and derivatives |
| US6001968A (en) | 1994-08-17 | 1999-12-14 | The Rockefeller University | OB polypeptides, modified forms and compositions |
| US6007998A (en) * | 1996-04-22 | 1999-12-28 | Merck & Co., Inc. | Leptin assay |
| WO1997040380A1 (en) * | 1996-04-22 | 1997-10-30 | Merck & Co., Inc. | Leptin assay |
| US6001816A (en) * | 1996-06-20 | 1999-12-14 | Merck & Co., Inc. | Gene therapy for leptin deficiency |
| EP0951564A1 (en) * | 1996-11-01 | 1999-10-27 | Smithkline Beecham Plc | Method for the detection of compounds that modulate the effects of the obese (ob) protein |
| IT1293533B1 (en) * | 1997-07-14 | 1999-03-01 | Angeletti P Ist Richerche Bio | METHOD FOR THE SELECTION OF MOLECULES ABLE TO MIMIC, INHIBIT OR ENHANCE THE EFFECTS OF THE INTERACTION BETWEEN LEPTIN AND CELLS THAT |
| WO1999023493A1 (en) * | 1997-10-31 | 1999-05-14 | The Rockefeller University | Methods of identifying agents that modulate leptin activity |
| CA2320226A1 (en) * | 1998-02-11 | 1999-08-19 | Beth Israel Deaconess Medical Center | Methods and compositions for modulating leptin activity |
| GB9814199D0 (en) * | 1998-06-30 | 1998-08-26 | Univ Buckingham | Novel method |
| GB9828087D0 (en) * | 1998-12-18 | 1999-02-17 | Smithkline Beecham Plc | Novel method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU7795094A (en) * | 1993-09-15 | 1995-04-03 | New York University | Dna sequence which binds transcriptional regulatory proteins activated in response to various cytokines and uses thereof |
| CA2165057A1 (en) * | 1994-04-14 | 1995-10-26 | H. Martin Seidel | Dna spacer regulatory elements responsive to cytokines and methods for their use |
-
1996
- 1996-05-28 TR TR97/01469T patent/TR199701469T1/en unknown
- 1996-05-28 PL PL96323638A patent/PL323638A1/en unknown
- 1996-05-28 HU HU9801744A patent/HUP9801744A3/en unknown
- 1996-05-28 CA CA002222409A patent/CA2222409A1/en not_active Abandoned
- 1996-05-28 KR KR1019970708613A patent/KR19990022134A/en not_active Application Discontinuation
- 1996-05-28 BR BR9608686A patent/BR9608686A/en active Search and Examination
- 1996-05-28 CZ CZ973790A patent/CZ379097A3/en unknown
- 1996-05-28 MX MX9709360A patent/MX9709360A/en unknown
- 1996-05-28 CN CN96195983A patent/CN1192248A/en active Pending
- 1996-05-28 EP EP96917454A patent/EP0832284A1/en not_active Withdrawn
- 1996-05-28 NZ NZ309777A patent/NZ309777A/en unknown
- 1996-05-28 AU AU60023/96A patent/AU715215B2/en not_active Ceased
- 1996-05-28 WO PCT/EP1996/002291 patent/WO1996038586A1/en not_active Application Discontinuation
- 1996-05-28 JP JP8536176A patent/JPH11505725A/en active Pending
-
1997
- 1997-11-28 NO NO975504A patent/NO975504L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| NO975504D0 (en) | 1997-11-28 |
| JPH11505725A (en) | 1999-05-25 |
| CA2222409A1 (en) | 1996-12-05 |
| KR19990022134A (en) | 1999-03-25 |
| BR9608686A (en) | 1999-07-06 |
| TR199701469T1 (en) | 1998-03-21 |
| AU6002396A (en) | 1996-12-18 |
| EP0832284A1 (en) | 1998-04-01 |
| MX9709360A (en) | 1998-02-28 |
| AU715215B2 (en) | 2000-01-20 |
| HUP9801744A3 (en) | 2000-06-28 |
| NZ309777A (en) | 2000-01-28 |
| NO975504L (en) | 1997-11-28 |
| CZ379097A3 (en) | 1998-05-13 |
| WO1996038586A1 (en) | 1996-12-05 |
| CN1192248A (en) | 1998-09-02 |
| PL323638A1 (en) | 1998-04-14 |
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